##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936391_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 583747 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21344006907102 33.0 31.0 34.0 31.0 34.0 2 32.300058073103585 33.0 31.0 34.0 31.0 34.0 3 32.30093516540556 34.0 31.0 34.0 31.0 34.0 4 35.92886301771144 37.0 35.0 37.0 35.0 37.0 5 35.96105847224911 37.0 35.0 37.0 35.0 37.0 6 36.01478380188678 37.0 36.0 37.0 35.0 37.0 7 35.981310396455996 37.0 36.0 37.0 35.0 37.0 8 35.96173856139732 37.0 36.0 37.0 35.0 37.0 9 37.598337978610594 39.0 38.0 39.0 35.0 39.0 10-11 37.522033517945275 39.0 37.0 39.0 35.0 39.0 12-13 37.43455897846156 39.0 37.0 39.0 35.0 39.0 14-15 38.61256331938323 40.0 38.0 41.0 35.0 41.0 16-17 38.566181068168234 40.0 38.0 41.0 35.0 41.0 18-19 38.61108322612365 40.0 38.0 41.0 35.0 41.0 20-21 38.53514193648961 40.0 38.0 41.0 35.0 41.0 22-23 38.593776927333245 40.0 38.0 41.0 35.0 41.0 24-25 38.58722186152563 40.0 38.0 41.0 35.0 41.0 26-27 38.458694434403945 40.0 38.0 41.0 35.0 41.0 28-29 38.28204941524325 40.0 38.0 41.0 34.5 41.0 30-31 37.98965819096287 40.0 37.0 41.0 34.0 41.0 32-33 37.68232299266634 40.0 36.0 41.0 34.0 41.0 34-35 37.447230563925814 39.5 35.0 41.0 33.0 41.0 36-37 37.22977420012437 39.0 35.0 41.0 33.0 41.0 38-39 36.97194589436862 39.0 35.0 41.0 33.0 41.0 40-41 36.69692863517928 39.0 35.0 40.5 32.5 41.0 42-43 36.40825049207962 39.0 35.0 40.0 31.5 41.0 44-45 36.04534498678366 38.0 35.0 40.0 30.5 41.0 46-47 35.59446643837141 38.5 35.0 40.0 29.5 41.0 48-49 35.27084079232955 38.5 35.0 40.0 27.5 41.0 50-51 34.24458455461013 37.5 34.0 39.5 25.5 40.5 52-53 34.33039741531863 38.0 34.5 39.5 26.0 40.5 54-55 34.71175697690951 38.0 35.0 40.0 26.5 41.0 56-57 34.58416317342959 38.0 35.0 40.0 26.0 41.0 58-59 34.34082230829452 37.5 35.0 40.0 26.0 41.0 60-61 34.02417913925039 37.0 34.0 40.0 25.0 41.0 62-63 33.62994413675788 36.0 34.0 40.0 23.0 41.0 64-65 33.21229402463739 36.0 33.5 39.0 22.0 41.0 66-67 32.74779227987467 35.0 33.0 39.0 19.5 41.0 68-69 32.36793593800054 35.0 33.0 38.5 17.5 40.0 70-71 31.953459289726545 35.0 33.0 37.5 13.0 40.0 72-73 31.548794255045422 35.0 32.5 37.0 7.5 39.0 74-75 31.138673089540504 35.0 32.0 36.5 4.0 39.0 76-77 30.675608611264813 35.0 31.5 36.0 2.0 38.5 78-79 30.231931812925804 35.0 31.0 35.5 2.0 37.0 80-81 29.826906176819755 34.5 31.0 35.0 2.0 37.0 82-83 29.449900384927034 34.0 30.0 35.0 2.0 36.5 84-85 29.142670540491 34.0 30.0 35.0 2.0 36.0 86-87 28.807480809323216 34.0 29.0 35.0 2.0 36.0 88-89 28.394163053514625 34.0 29.0 35.0 2.0 35.0 90-91 28.01039748384146 34.0 29.0 35.0 2.0 35.0 92-93 27.615014723844403 34.0 27.0 35.0 2.0 35.0 94-95 27.168398295837065 34.0 27.0 35.0 2.0 35.0 96-97 26.63704909832513 33.5 25.0 35.0 2.0 35.0 98-99 25.97915706633182 33.0 24.5 35.0 2.0 35.0 100-101 23.300557433271607 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3047062445819151 1101 2 0.3313624115770395 1101 3 0.3119498089555748 1101 4 0.4039942227399749 1101 5 0.3455013784510328 1101 6 0.3613058618842331 1101 7 0.36765580655894325 1101 8 0.3475780520731817 1101 9 0.25176406949652375 1101 10-11 0.599347574582417 1101 12-13 0.6845425893391379 1101 14-15 0.706090675482713 1101 16-17 0.6730845266103316 1101 18-19 0.6694332920715169 1101 20-21 0.6800609171781318 1101 22-23 0.6131840249053795 1101 24-25 0.6188009840761879 1101 26-27 0.5490438828071333 1101 28-29 0.5573422693530503 1101 30-31 0.5934473409339631 1101 32-33 0.5175417392633079 1101 34-35 0.4763120942869463 1101 36-37 0.3710861790595814 1101 38-39 0.5114163237416065 1101 40-41 0.6733368412903573 1101 42-43 0.6885310861011646 1101 44-45 0.6244822850509664 1101 46-47 0.9095792756219865 1101 48-49 1.2896154018332524 1101 50-51 1.0447095262874697 1101 52-53 0.9749576929577799 1101 54-55 1.00856121910072 1101 56-57 0.8016948446151133 1101 58-59 1.2594568373732855 1101 60-61 1.2174180197199647 1101 62-63 1.0495537157746853 1101 64-65 1.1119599786765448 1101 66-67 1.242705092112928 1101 68-69 1.0715391656446798 1101 70-71 1.2442546391032216 1101 72-73 1.130195366031021 1101 74-75 1.2035587955580773 1101 76-77 1.2844317642141831 1101 78-79 1.2824175388022496 1101 80-81 1.254385929969395 1101 82-83 1.1700330011039775 1101 84-85 0.9657038883824249 1101 86-87 1.118827044815614 1101 88-89 1.144022636057489 1101 90-91 0.9385488230615309 1101 92-93 1.0466358052522082 1101 94-95 1.1040790383755272 1101 96-97 0.6969998273170965 1101 98-99 0.497866561098288 1101 100-101 0.9364250182428044 1102 1 0.17815424389399226 1102 2 0.19258569084151844 1102 3 0.3092489987124978 1102 4 0.1892455876231267 1102 5 0.19700068789298797 1102 6 0.08580566796640454 1102 7 -0.020509765919811684 1102 8 0.09163635728403818 1102 9 0.13540460025224377 1102 10-11 0.2082609503495334 1102 12-13 0.1174496544411241 1102 14-15 0.2294871681147157 1102 16-17 0.07513057902254872 1102 18-19 0.12527179800926547 1102 20-21 -0.008722033496546544 1102 22-23 0.018961021162148484 1102 24-25 -0.09205262288308802 1102 26-27 -0.2601260611585161 1102 28-29 -0.3425777066397515 1102 30-31 0.037961396905132005 1102 32-33 0.033373681828528845 1102 34-35 0.07405808475075304 1102 36-37 -0.19919718665188668 1102 38-39 -0.15011020439227707 1102 40-41 -0.052075519259908276 1102 42-43 -0.07487337383331294 1102 44-45 -0.07558650751662555 1102 46-47 -4.6742259800680586E-4 1102 48-49 -0.04161203657370294 1102 50-51 0.20956863314233232 1102 52-53 0.1937092833471752 1102 54-55 0.0637619898582642 1102 56-57 0.08737528558950913 1102 58-59 0.3361570404166585 1102 60-61 0.19331956211249945 1102 62-63 0.1819346581275667 1102 64-65 0.1332979590180159 1102 66-67 0.43807861645093027 1102 68-69 0.2788276679848565 1102 70-71 0.41510677193604906 1102 72-73 0.11730114688808868 1102 74-75 0.050262806761434575 1102 76-77 0.2745647690279043 1102 78-79 0.2769167657633176 1102 80-81 0.5557605528825853 1102 82-83 0.44351943299845686 1102 84-85 0.2638371880214372 1102 86-87 0.2866107132494804 1102 88-89 0.24077095879992783 1102 90-91 0.17954743843561616 1102 92-93 0.16598827766059898 1102 94-95 0.03606038716612758 1102 96-97 0.23767167799510602 1102 98-99 0.25865269167430327 1102 100-101 0.140030836830487 1103 1 0.10738757153303169 1103 2 -8.185770876565357E-4 1103 3 0.09654457076213419 1103 4 0.09665467361970315 1103 5 0.14696704509237435 1103 6 0.1516496839057737 1103 7 0.17843249048364385 1103 8 0.18800816426849565 1103 9 0.05249165666407407 1103 10-11 0.2655086206291699 1103 12-13 -0.0641395408161074 1103 14-15 0.0612208705310735 1103 16-17 0.11440811295296527 1103 18-19 0.2854185015377553 1103 20-21 0.21884536540945732 1103 22-23 0.06879071537872505 1103 24-25 0.2828074893060659 1103 26-27 0.133362226614679 1103 28-29 -0.018859009261099402 1103 30-31 0.08091174849855065 1103 32-33 0.01272432348214636 1103 34-35 0.31791173682493223 1103 36-37 0.3575501994942485 1103 38-39 0.39735229172922004 1103 40-41 0.13167605393322646 1103 42-43 0.14672286375109422 1103 44-45 0.2464535361318596 1103 46-47 0.4331272670689472 1103 48-49 0.3444521741104154 1103 50-51 0.15215797831049116 1103 52-53 0.0321244808286707 1103 54-55 0.22510885236206235 1103 56-57 0.2483360403038617 1103 58-59 0.1792630553310488 1103 60-61 0.23289778946810458 1103 62-63 0.4119869177732909 1103 64-65 0.37359558449868757 1103 66-67 0.420113709552588 1103 68-69 0.37870233547893406 1103 70-71 0.29224518155544743 1103 72-73 0.07245511935328608 1103 74-75 -0.09330653750028617 1103 76-77 0.07582210333275441 1103 78-79 0.19697850814662132 1103 80-81 0.28630786721093315 1103 82-83 0.036609759560231936 1103 84-85 0.17756345067234847 1103 86-87 0.15957427792210055 1103 88-89 0.0742216642528497 1103 90-91 0.08392605765140715 1103 92-93 0.06791166969161111 1103 94-95 0.37808652047528923 1103 96-97 0.5611506922087557 1103 98-99 0.29465048833233354 1103 100-101 0.5767143888129702 1104 1 0.27794999688637745 1104 2 0.2840908005170846 1104 3 0.2930037482867647 1104 4 0.3252457416397192 1104 5 0.3112582491452116 1104 6 0.38612227708104285 1104 7 0.44240111272736016 1104 8 0.3295219894609076 1104 9 0.4664842740871009 1104 10-11 0.47213688298127465 1104 12-13 0.4604419868649501 1104 14-15 0.2721348884065762 1104 16-17 0.26957303019666057 1104 18-19 0.27590702895539465 1104 20-21 0.22282441563334743 1104 22-23 0.1788004938653316 1104 24-25 0.4441570287068686 1104 26-27 0.39919273044127834 1104 28-29 0.33858256337934023 1104 30-31 0.4549000720473799 1104 32-33 0.4896312185781113 1104 34-35 0.531269474277984 1104 36-37 0.6146405043593219 1104 38-39 0.5971004325538587 1104 40-41 0.5459726704325334 1104 42-43 0.6917941095880309 1104 44-45 0.8509913312455026 1104 46-47 0.7968292361169702 1104 48-49 0.985662902300966 1104 50-51 0.7999754994821799 1104 52-53 0.9489261579654098 1104 54-55 0.9013173614495571 1104 56-57 0.7814764499899738 1104 58-59 0.8773171004131157 1104 60-61 0.9358825732769489 1104 62-63 1.1342778981116766 1104 64-65 1.0442238612839887 1104 66-67 1.296996789884119 1104 68-69 1.1132046971097367 1104 70-71 0.9891003697462146 1104 72-73 0.9975847474460444 1104 74-75 0.9466481422813402 1104 76-77 0.9888071036394592 1104 78-79 1.0210514462751448 1104 80-81 1.06141891257084 1104 82-83 1.06705386883392 1104 84-85 0.7860523317031713 1104 86-87 0.903966983387587 1104 88-89 0.789609971203511 1104 90-91 0.42492912831270147 1104 92-93 0.663659651751157 1104 94-95 0.6401003474718543 1104 96-97 0.6844326914943117 1104 98-99 0.37654801989591746 1104 100-101 0.4941132392093408 1105 1 0.12229447226386725 1105 2 0.0357123274626403 1105 3 0.10362525679540369 1105 4 0.0037280145429861022 1105 5 0.020069252297929552 1105 6 0.2504671211968983 1105 7 0.29444115771343604 1105 8 0.2274220244910552 1105 9 0.29429267763435973 1105 10-11 0.29057564475785824 1105 12-13 0.2373331537374952 1105 14-15 0.07644728354660657 1105 16-17 0.09390607468494494 1105 18-19 0.09428955813182682 1105 20-21 0.14204305904491576 1105 22-23 -0.0013936468821000858 1105 24-25 0.14199622190733407 1105 26-27 -0.011755111517459227 1105 28-29 -0.004354253302381039 1105 30-31 -0.039876749059203576 1105 32-33 0.0583723458112857 1105 34-35 -0.05154178699158507 1105 36-37 0.12343583613166231 1105 38-39 0.1334737063974032 1105 40-41 0.10206791555135908 1105 42-43 -0.14172144195237735 1105 44-45 0.39245596001816097 1105 46-47 0.6797889145771165 1105 48-49 0.4096179899685026 1105 50-51 0.3684843215474771 1105 52-53 0.5672561419252915 1105 54-55 0.6208927592260594 1105 56-57 0.613163981102268 1105 58-59 0.6130377073564617 1105 60-61 0.7496024058697373 1105 62-63 0.5793819745142628 1105 64-65 0.5801841481624237 1105 66-67 0.8038516360280354 1105 68-69 0.8126128720392174 1105 70-71 0.6421148058530726 1105 72-73 0.7968574110276059 1105 74-75 0.8427579525654529 1105 76-77 0.6030210456369147 1105 78-79 0.7931333430652785 1105 80-81 0.7479608842148622 1105 82-83 0.740682623051363 1105 84-85 0.5080272943684072 1105 86-87 0.49625942978224913 1105 88-89 0.534512163353071 1105 90-91 0.5349966074328911 1105 92-93 0.5874331357755658 1105 94-95 0.8120227649205525 1105 96-97 0.9837883212072498 1105 98-99 1.0115901482537417 1105 100-101 1.1477360936130019 1106 1 0.11301685051419241 1106 2 0.12700213850943243 1106 3 0.12634740577994563 1106 4 0.16148579525027174 1106 5 -0.030632874142149547 1106 6 0.1196557114034249 1106 7 0.2722176617924603 1106 8 0.30850950849021075 1106 9 0.29298395216102335 1106 10-11 0.20354635605492888 1106 12-13 0.20418132247710474 1106 14-15 0.05497463304318018 1106 16-17 0.2256765796428155 1106 18-19 0.22059111904965079 1106 20-21 -0.14947792832635542 1106 22-23 -0.047257310167097444 1106 24-25 0.07504297302023133 1106 26-27 -0.17470385151096224 1106 28-29 -0.008959629584836648 1106 30-31 0.05340194259743214 1106 32-33 -0.011955886358521184 1106 34-35 -0.15897381924163767 1106 36-37 0.02585554534623924 1106 38-39 -0.16216737802972148 1106 40-41 -0.323066300455217 1106 42-43 -0.4294509465472487 1106 44-45 -0.3958170955214726 1106 46-47 -0.23229052601438838 1106 48-49 0.02746824120558955 1106 50-51 -0.30598980007801657 1106 52-53 -0.5360643907355254 1106 54-55 -0.3830897783639742 1106 56-57 -0.31369259404913663 1106 58-59 -0.5018292229658456 1106 60-61 -0.43227508978376505 1106 62-63 -0.48587279707970765 1106 64-65 -0.5220215796608798 1106 66-67 -0.3013183072286836 1106 68-69 -0.384513663563844 1106 70-71 -0.3666861341366108 1106 72-73 -0.36930773675329576 1106 74-75 -0.21120356899508508 1106 76-77 -0.15588975087059964 1106 78-79 -0.26731735524006695 1106 80-81 -0.243530489660305 1106 82-83 -0.2383882773899728 1106 84-85 0.022570386781623597 1106 86-87 -0.11242496677112257 1106 88-89 0.005170920350455077 1106 90-91 -0.06293904530587824 1106 92-93 -0.009688623457158485 1106 94-95 -0.06491118743037916 1106 96-97 -0.14393659807209502 1106 98-99 -0.2675781901425438 1106 100-101 -0.5577844352267256 1107 1 0.07120239259921846 1107 2 0.04814338303936694 1107 3 -0.005428274161893398 1107 4 0.02741079999796625 1107 5 0.02168982230534766 1107 6 -0.05555987493218595 1107 7 0.2438825825314268 1107 8 0.1602485954210735 1107 9 0.3387680983206991 1107 10-11 0.14255221869635903 1107 12-13 0.1438334846707292 1107 14-15 -0.07949447220461536 1107 16-17 -0.14175849941400998 1107 18-19 -0.1331869255521667 1107 20-21 -0.11338725443910391 1107 22-23 -0.16988780039500995 1107 24-25 0.15182193040530478 1107 26-27 0.22304502486714028 1107 28-29 0.03920959675478031 1107 30-31 -0.10892747209817344 1107 32-33 -0.21541874506498715 1107 34-35 0.0631837389916825 1107 36-37 0.0630846457748575 1107 38-39 -0.09727400214067927 1107 40-41 0.02665424901172031 1107 42-43 0.07021722508867612 1107 44-45 0.12947435188321776 1107 46-47 0.22425731546763217 1107 48-49 0.0930773453506859 1107 50-51 0.023163516878319967 1107 52-53 -0.10937395494789115 1107 54-55 0.21468625688947185 1107 56-57 0.13426548734988586 1107 58-59 0.15911263866274794 1107 60-61 0.23095281402072487 1107 62-63 0.17758891809516797 1107 64-65 0.2869381945733096 1107 66-67 0.15072381557435932 1107 68-69 0.24245101560407534 1107 70-71 0.22054899025308217 1107 72-73 0.27128028352805345 1107 74-75 0.29529209885799546 1107 76-77 0.42253507520028677 1107 78-79 0.38732997757459486 1107 80-81 0.4320730806086921 1107 82-83 0.39388565560316025 1107 84-85 0.3792501428009878 1107 86-87 0.38250163279474236 1107 88-89 0.12576449492811292 1107 90-91 0.044644815379299985 1107 92-93 -0.11736539608427776 1107 94-95 -0.011076374653047338 1107 96-97 -0.12176433728971148 1107 98-99 0.1743681823097809 1107 100-101 -0.3556805412974491 1108 1 0.16067859303546328 1108 2 0.13876356752813734 1108 3 0.14083520926954662 1108 4 0.1868343686306062 1108 5 0.16499516786963397 1108 6 0.2045108130376292 1108 7 0.2885982726141947 1108 8 0.19368665167264965 1108 9 0.20545127022990783 1108 10-11 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-0.7482105776663062 2316 82-83 -0.6680216281040678 2316 84-85 -0.2558524574740346 2316 86-87 -0.3551812164266863 2316 88-89 -0.867546794884845 2316 90-91 -0.7207118490770803 2316 92-93 -1.4835644909055326 2316 94-95 -1.1933359393306056 2316 96-97 -1.5811136232487613 2316 98-99 -2.5514547782566446 2316 100-101 -3.5184191297704395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1037.0 3 341.0 4 262.0 5 279.0 6 308.0 7 348.0 8 372.0 9 483.0 10 648.0 11 965.0 12 1620.0 13 2393.0 14 2652.0 15 3753.0 16 5664.0 17 9036.0 18 6802.0 19 3834.0 20 3635.0 21 3629.0 22 3884.0 23 4199.0 24 4608.0 25 4755.0 26 5523.0 27 6431.0 28 7577.0 29 9216.0 30 11543.0 31 15005.0 32 20270.0 33 28359.0 34 43473.0 35 73588.0 36 93443.0 37 114464.0 38 79494.0 39 9854.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.78233724936565 18.266346165377982 14.199315022726921 20.752001562529447 2 15.701311183868993 21.182652555977597 39.362928773230124 23.753107486923284 3 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20.902324584895553 22-23 21.060986098404246 25.525378893012196 32.02532578117236 21.38830922741119 24-25 21.112374054255923 25.909340478146277 32.38935233287108 20.58893313472672 26-27 21.02499224827449 25.58626171538405 32.7197335804749 20.669012455866557 28-29 20.079520819396777 25.95176159514447 33.50161414244025 20.4671034430185 30-31 20.023743976208056 26.673941757019975 32.84609072391718 20.456223542854794 32-33 19.631131462951256 27.06349328714768 32.14305904603319 21.162316203867878 34-35 20.558906512581647 26.58009377350119 31.82414641959616 21.036853294321 36-37 20.968195071143956 26.39384252740062 31.972124862525824 20.6658375389296 38-39 21.086397165890645 26.080606976321896 32.19970672175912 20.633289136028342 40-41 20.151352277350693 26.816248741943085 32.72481209447739 20.307586886228826 42-43 20.00354624205415 27.536398234416883 31.555815194533647 20.904240328995325 44-45 19.824662837858096 27.736762986470687 31.085385857391874 21.353188318279347 46-47 20.26242825365038 27.770815970452624 30.17376728186583 21.792988494031164 48-49 20.75077366895962 27.916322980344997 29.728245833579585 21.604657517115793 50-51 21.022886417619087 28.305014043295664 29.494775414339326 21.177324124745923 52-53 20.637215684729334 28.191688923075343 29.667293101055805 21.50380229113952 54-55 19.95086655936572 28.025762506285158 30.0098592381747 22.013511696174426 56-57 20.73139812760709 28.77121003177757 28.66791150245398 21.829480338161357 58-59 20.774536290702514 27.807653366271666 29.184176578718795 22.23363376430702 60-61 20.65321295804567 27.592294382676926 29.930275983759614 21.82421667551779 62-63 21.445757333070663 27.583994655177584 29.732801706231772 21.237446305519985 64-65 21.00794270086091 27.875942087577847 30.00521633217329 21.110898879387953 66-67 20.62796788113312 28.17374274922491 29.66429050560744 21.53399886403453 68-69 20.46958558870431 28.30632315049583 29.9424435896623 21.281647671137563 70-71 20.618981635015754 28.230625331526916 29.210016890198286 21.940376143259048 72-73 20.598348732937794 28.06143165779469 28.9579096514658 22.382309957801713 74-75 20.39779639815624 28.45627923713566 29.13569458009904 22.01022978460906 76-77 20.752004537249398 27.73556471877364 29.665009462598107 21.847421281378857 78-79 20.429989579334173 27.766446827181486 30.074418636538148 21.729144956946197 80-81 20.29213710437803 28.815045549456393 29.41462862707047 21.478188719095115 82-83 20.294243914477487 28.302703277060402 29.085399973093196 22.31765283536891 84-85 20.317996076449273 27.697098457136494 28.97902014032262 23.00588532609161 86-87 20.804454915399443 27.667680445491538 29.246605268794173 22.281259370314842 88-89 21.122579583948024 27.09692287394903 29.173070563662872 22.60742697844007 90-91 20.451166732215828 27.481207098502825 29.66420835893178 22.40341781034957 92-93 20.435926916572285 28.05903630353988 29.272781169519902 22.232255610367936 94-95 20.225206124852768 27.824649320055684 28.960959417496518 22.98918513759503 96-97 20.362316171273932 27.376398820206578 28.614873518297852 23.646411490221645 98-99 20.623486402690155 27.371838049158388 28.418266173745977 23.586409374405488 100-101 21.093484161887563 27.17288171620907 28.729565419371205 23.00406870253216 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2012.0 1 1512.0 2 1965.0 3 2507.5 4 1686.5 5 1458.5 6 2502.5 7 4701.5 8 6805.0 9 7047.5 10 4963.5 11 3011.0 12 2619.0 13 2622.5 14 2504.5 15 2253.5 16 1947.0 17 1766.5 18 1734.5 19 1659.5 20 1604.0 21 1691.5 22 1828.5 23 1960.5 24 2085.0 25 2250.5 26 2644.5 27 3141.0 28 3608.5 29 4357.0 30 5280.0 31 6181.0 32 7728.0 33 9099.5 34 10184.0 35 11017.5 36 11673.5 37 12497.5 38 12855.5 39 12951.0 40 12955.5 41 13586.5 42 14087.5 43 14747.5 44 16005.0 45 16879.5 46 17148.0 47 19119.5 48 31728.0 49 32008.5 50 20747.0 51 18024.5 52 15786.0 53 15841.5 54 16223.0 55 15758.0 56 14928.0 57 13485.5 58 12781.0 59 12189.0 60 10729.0 61 9344.0 62 8125.0 63 6497.5 64 4981.0 65 4235.5 66 3378.5 67 2610.0 68 1966.5 69 1323.0 70 1050.0 71 870.0 72 764.0 73 759.0 74 642.0 75 507.0 76 339.0 77 206.0 78 158.0 79 101.5 80 63.0 81 54.5 82 37.5 83 21.0 84 13.0 85 7.5 86 5.0 87 4.5 88 5.0 89 4.5 90 2.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013361953037874285 2 0.013019338857415968 3 0.018158551564290696 4 0.01918639410566564 5 0.03134919751193582 6 0.04539637891072674 7 0.007194897789624615 8 0.024154299722311205 9 0.03563187476766476 10-11 0.04171327647079985 12-13 0.029550473064529667 14-15 0.023640378451623735 16-17 0.026895213165977726 18-19 0.024753874538113257 20-21 0.05301954442592425 22-23 0.012505417586728497 24-25 0.002312645718093626 26-27 0.001027842541374945 28-29 8.565354511457875E-5 30-31 0.0034261418045831503 32-33 0.00274091344366652 34-35 0.0 36-37 1.713070902291575E-4 38-39 1.713070902291575E-4 40-41 0.001627417357176996 42-43 0.0052248662519893034 44-45 0.0024839528083227835 46-47 0.0011134960864895237 48-49 4.282677255728938E-4 50-51 0.0028265669887810986 52-53 0.0034261418045831503 54-55 0.0052248662519893034 56-57 3.42614180458315E-4 58-59 0.005139212706874724 60-61 0.0029122205338956778 62-63 7.708819060312087E-4 64-65 5.995748158020512E-4 66-67 0.017558976748488643 68-69 0.03982889847827912 70-71 0.046681182087445414 72-73 0.012676724676957655 74-75 0.026895213165977726 76-77 0.022869496545592526 78-79 0.04985036325668483 80-81 0.07280551334739194 82-83 0.04308373319263311 84-85 0.01576025230108249 86-87 0.020899465007957214 88-89 0.020214236647040584 90-91 0.03691667794438344 92-93 0.02398299263208205 94-95 0.010192771868634872 96-97 0.014646756214592967 98-99 0.04899382780553904 100-101 0.06706672582471516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 583747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.001308805094325 #Duplication Level Percentage of deduplicated Percentage of total 1 71.32127841343306 29.24265760603277 2 14.125072837429297 11.58292946603777 3 5.481592653288678 6.742574193636763 4 2.6620518518920577 4.365904401383979 5 1.558120584136926 3.194249161288602 6 1.0054709926092396 2.4735375997521682 7 0.6539832554148157 1.8769918586036611 8 0.4703369008220707 1.5427542810429393 9 0.3343750596197151 1.2338833568570784 >10 2.1237186307698455 16.611194654348573 >50 0.17097066478887155 4.827121094019384 >100 0.07919964615011757 5.760931490360839 >500 0.006285686202390285 1.703194567967402 >1k 0.006285686202390285 4.903578429313997 >5k 8.380914936520379E-4 2.1851335131049554 >10k+ 4.1904574682601894E-4 1.7533643262491012 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10205 1.7481888557885523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7556 1.294396373771514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5162 0.884287199762911 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3786 0.6485686436075903 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3326 0.5697673821021778 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2681 0.4592743089043712 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2673 0.45790385218253793 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2393 0.40993786691837386 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1914 0.32788177069860747 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1525 0.26124331259946515 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1515 0.2595302416971736 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1507 0.25815978497534037 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1442 0.2470248241104451 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 0.2196156896737799 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1209 0.20711027208705138 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1155 0.19785968921467692 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1118 0.19152132687619808 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1014 0.1737053894923657 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 900 0.15417638120624175 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 861 0.14749540468730463 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 815 0.13961527853676337 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 798 0.13670305800286767 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 736 0.12608201840865993 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 728 0.12471156168682665 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 644 0.11032176610757743 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 620 0.10621039594207765 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.5696063534373626E-4 0.0 22-23 0.0 0.0 0.0 5.139212706874725E-4 0.0 24-25 0.0 0.0 0.0 0.0014561102669478389 0.0 26-27 0.0 0.0 0.0 0.00205568508274989 0.0 28-29 0.0 0.0 0.0 0.003340488259468571 0.0 30-31 0.0 0.0 0.0 0.009507543507718241 0.0 32-33 0.0 0.0 0.0 0.017901590928946956 0.0 34-35 0.0 0.0 0.0 0.02852263052315472 0.0 36-37 0.0 0.0 0.0 0.044111575734008056 0.0 38-39 0.0 0.0 0.0 0.06552496201265275 0.0 40-41 0.0 0.0 0.0 0.09242017517863046 0.0 42-43 0.0 0.0 0.0 0.12376937269056629 0.0 44-45 0.0 0.0 0.0 0.15803079073639778 0.0 46-47 0.0 0.0 0.0 0.2023993271057496 0.0 48-49 0.0 0.0 0.0 0.24334172167051824 0.0 50-51 0.0 0.0 0.0 0.2910507462993386 0.0 52-53 0.0 0.0 0.0 0.33824584965747145 0.0 54-55 0.0 0.0 0.0 0.3856979136509481 0.0 56-57 0.0 0.0 0.0 0.4489102299455072 0.0 58-59 0.0 0.0 0.0 0.5253131921877114 0.0 60-61 0.0 0.0 0.0 0.6188468634528315 0.0 62-63 0.0 0.0 0.0 0.7041577943869519 0.0 64-65 0.0 0.0 0.0 0.7886978434150411 0.0 66-67 0.0 0.0 0.0 0.8871994202968068 0.0 68-69 0.0 0.0 0.0 1.0009473282089671 0.0 70-71 0.0 0.0 0.0 1.1343098979523663 0.0 72-73 0.0 0.0 0.0 1.275895208026765 0.0 74-75 0.0 0.0 0.0 1.4260458726126215 0.0 76-77 0.0 0.0 0.0 1.5809931357248945 0.0 78-79 0.0 0.0 0.0 1.751272383412677 0.0 80-81 0.0 0.0 0.0 1.9532434427928538 0.0 82-83 0.0 0.0 0.0 2.1663494630379256 0.0 84-85 0.0 0.0 0.0 2.402753247554163 0.0 86-87 0.0 0.0 0.0 2.643354055781015 0.0 88-89 0.0 0.0 0.0 2.895261131962991 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 155 3.6379788E-11 33.716675 2 TAAGGTG 190 1.2732926E-11 30.008795 5 GTAAGGT 205 3.092282E-11 27.81303 4 GGTAAGG 190 3.929017E-10 27.508062 3 GTACATG 6105 0.0 27.077143 1 ATAGGTA 90 8.926952E-4 26.396626 8 TAGATAG 90 8.926952E-4 26.396626 5 ATGGGAC 835 0.0 26.175339 5 TACATGG 6135 0.0 26.174948 2 AGGTGTG 265 1.8189894E-12 25.101698 7 CCCTATA 95 0.0012243494 25.005186 2 ACATGGG 6180 0.0 24.525799 3 AAGGTGT 255 1.8189894E-11 24.222786 6 GAGTACT 5170 0.0 24.166386 12-13 AGTACTT 5235 0.0 23.9117 12-13 TAAGGTA 140 1.7735043E-5 23.756966 4 GTGTAAG 140 1.7770377E-5 23.750856 1 CATGGGG 1705 0.0 23.408623 4 GATGTGC 225 2.7957867E-9 23.229033 8 GTATCAA 10960 0.0 22.927378 1 >>END_MODULE