FastQCFastQC Report
Thu 26 May 2016
SRR936391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583747
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81121.3896431159389255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74941.2837753341773064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55470.9502404295011366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.9327671062977626No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35840.6139646113813004No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA26710.45756123800207965No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20770.3558048264059601No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20160.3453550939019815No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.33422013303708625No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.2915646675700261No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14900.25524756444144464No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14260.24428391066677857No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12320.21105033516232202No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC10780.18466904326703176No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA10710.18346989363542768No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT10360.17747414547740717No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG9570.16394088534930373No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.1462962550557005No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT8510.14578233378501304No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8080.13841612890515925No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA7810.133790837468972No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG7730.13242038074713874No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG7420.12710986095003488No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6470.1108356873782649No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG6190.1060390888518485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG1753.6379788E-1232.572633
GTAAGGT2300.028.9141144
GTAAGCG504.95482E-528.49861194-95
TAAGGTG2207.6397555E-1125.9078315
ACTTGAC1501.0590647E-625.33218
GAGTGCA2950.024.1513659
AGGTAAG1401.777026E-523.750882
GGGATAG1000.001653142923.7488447
ATGGGAG8050.023.6013365
ATCCCGA1252.2547998E-422.798896
TCCCGAT1252.2547998E-422.798897
GCAATAG1050.00219337122.6218221
AGGTGTG2955.456968E-1222.5412757
GAGTACT62800.022.4252612-13
TGTAGGA3007.2759576E-1222.1674862
GTGTAGG2154.40632E-822.0957341
CGTATAG652.942516E-421.92200914-15
AGTACTT63850.021.90770312-13
TGGGAGT2858.367351E-1121.6656116
CCCTACA1553.901072E-521.4524062
Produced by FastQC (version 0.11.5)