##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936391_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 583747 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.065696269102883 31.0 31.0 33.0 30.0 34.0 2 31.167283086679674 31.0 31.0 34.0 28.0 34.0 3 31.178061728796894 31.0 31.0 34.0 28.0 34.0 4 35.068877441768436 35.0 35.0 37.0 32.0 37.0 5 34.90457852460055 35.0 35.0 37.0 32.0 37.0 6 35.05216472204568 36.0 35.0 37.0 32.0 37.0 7 34.93707890575883 36.0 35.0 37.0 32.0 37.0 8 34.925489981104825 36.0 35.0 37.0 32.0 37.0 9 36.30786453720533 38.0 35.0 39.0 32.0 39.0 10-11 36.1706629755699 38.0 35.0 39.0 31.5 39.0 12-13 35.960229345932404 37.0 35.0 39.0 30.0 39.0 14-15 36.6328803402844 38.0 35.5 40.0 31.0 41.0 16-17 36.581375150536104 38.0 35.5 40.0 30.5 41.0 18-19 36.84982877856332 38.5 36.0 40.0 31.0 41.0 20-21 36.88514630482041 39.0 36.0 40.0 31.0 41.0 22-23 36.69955220326614 38.5 35.0 40.0 30.5 41.0 24-25 36.469302625966385 38.0 35.0 40.0 30.0 41.0 26-27 36.04476082960598 38.0 34.5 40.0 29.5 41.0 28-29 35.82039479432014 38.0 34.0 40.0 29.0 41.0 30-31 35.49968308188308 38.0 34.0 40.0 28.0 41.0 32-33 35.05314288553089 37.0 33.5 40.0 27.0 41.0 34-35 34.6973157892032 37.0 33.0 40.0 26.5 41.0 36-37 34.30387736468025 37.0 33.0 40.0 25.0 41.0 38-39 33.871234456022904 36.0 33.0 39.5 24.0 41.0 40-41 33.423803462801516 36.0 32.5 39.0 22.5 40.0 42-43 32.937448929073724 35.5 31.5 39.0 20.5 40.0 44-45 32.39098102431362 35.0 31.0 39.0 18.5 40.0 46-47 31.62699165905778 35.0 30.0 39.0 12.0 40.0 48-49 30.9126864891811 35.0 29.5 38.5 7.5 40.0 50-51 30.41205093987635 35.0 28.0 38.0 4.0 40.0 52-53 29.971901354525162 34.5 27.5 38.0 2.0 40.0 54-55 29.476433283597174 34.0 27.0 38.0 2.0 40.0 56-57 29.005228292393795 34.0 26.0 38.0 2.0 40.0 58-59 28.47427567079574 33.0 25.5 37.0 2.0 39.5 60-61 27.901732257296395 33.0 24.5 37.0 2.0 39.0 62-63 27.365273825818377 33.0 23.0 36.0 2.0 39.0 64-65 26.845248883506038 32.0 22.0 36.0 2.0 39.0 66-67 26.206650312549787 31.5 21.0 35.0 2.0 38.0 68-69 25.65448302089775 31.0 20.0 35.0 2.0 38.0 70-71 24.99378069608923 31.0 18.0 35.0 2.0 37.0 72-73 24.239997807269248 30.0 14.0 34.0 2.0 36.0 74-75 23.542412209398936 29.0 9.0 34.0 2.0 36.0 76-77 22.628245626958254 28.5 8.0 32.5 2.0 35.0 78-79 23.251807718069642 29.5 7.0 34.0 2.0 35.0 80-81 22.978613166320343 29.0 4.0 34.0 2.0 35.0 82-83 22.375852038640026 29.0 2.0 34.0 2.0 35.0 84-85 21.715418665963163 29.0 2.0 33.5 2.0 35.0 86-87 20.9766028776165 27.5 2.0 33.0 2.0 35.0 88-89 20.104982124105135 26.5 2.0 33.0 2.0 35.0 90-91 19.164670653553678 25.0 2.0 32.5 2.0 34.0 92-93 18.236746398696695 24.5 2.0 32.0 2.0 34.0 94-95 17.13243579838526 19.5 2.0 31.5 2.0 34.0 96-97 15.915065944664383 9.0 2.0 31.0 2.0 34.0 98-99 14.518701595040318 2.0 2.0 31.0 2.0 34.0 100-101 11.668467675208609 2.0 2.0 27.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39563658637541366 1101 2 0.5535280916548722 1101 3 0.4247108597600686 1101 4 0.5024970044027839 1101 5 0.4987038419388057 1101 6 0.4006977500780948 1101 7 0.46615686178561333 1101 8 0.39545476966122806 1101 9 0.6276979948277273 1101 10-11 0.4427354729620703 1101 12-13 0.5152789773587259 1101 14-15 0.46297141660254226 1101 16-17 0.6584360189741787 1101 18-19 0.7293432676866018 1101 20-21 0.4205348001885767 1101 22-23 0.5701772170395714 1101 24-25 0.6272641561014822 1101 26-27 0.42978350153823186 1101 28-29 0.6814250749470716 1101 30-31 0.9372040895317326 1101 32-33 1.1071993133002636 1101 34-35 0.8371330086156519 1101 36-37 0.6950438492744198 1101 38-39 0.6997241113310935 1101 40-41 0.7463460945564506 1101 42-43 0.8801963231814085 1101 44-45 1.036194664591541 1101 46-47 1.1049657796116215 1101 48-49 1.008018216080071 1101 50-51 1.0847637762346274 1101 52-53 1.329769970767675 1101 54-55 1.4803189910239247 1101 56-57 1.393449069103859 1101 58-59 1.2814839097990998 1101 60-61 1.1509836570818202 1101 62-63 1.435164382184979 1101 64-65 1.2787365456449997 1101 66-67 1.7242762627678445 1101 68-69 1.403197621838327 1101 70-71 1.163776712371721 1101 72-73 1.529234803574532 1101 74-75 1.525073447387438 1101 76-77 1.2714279986140937 1101 78-79 1.548183556326368 1101 80-81 1.3984551237772251 1101 82-83 1.194102246243176 1101 84-85 1.50592671995269 1101 86-87 1.8480239682033144 1101 88-89 1.5477330291229876 1101 90-91 1.4924251011431053 1101 92-93 1.5161136905072503 1101 94-95 1.536857537562728 1101 96-97 1.2771325737287373 1101 98-99 0.6490115446285838 1101 100-101 0.7537800852184038 1102 1 0.31962957374530987 1102 2 0.5597527660747446 1102 3 0.4245125546545019 1102 4 0.42390851557543385 1102 5 0.36288239374996323 1102 6 0.497544347917092 1102 7 0.28928625674445385 1102 8 0.1333024397517235 1102 9 0.5158714644124629 1102 10-11 0.6654426359881711 1102 12-13 0.4450991514811449 1102 14-15 0.5832083648800079 1102 16-17 0.5801958802659044 1102 18-19 0.3979271761924892 1102 20-21 0.5344523087744832 1102 22-23 0.5897953831911735 1102 24-25 0.5696652974965346 1102 26-27 0.5708810939738598 1102 28-29 0.6550899814365607 1102 30-31 1.0410097926461717 1102 32-33 0.7451222691343844 1102 34-35 0.9090510752637257 1102 36-37 1.0355925050992312 1102 38-39 0.9639261789689328 1102 40-41 1.1405836640729277 1102 42-43 1.343383524955982 1102 44-45 1.5236680996044996 1102 46-47 1.3914806814505916 1102 48-49 1.5485426541132306 1102 50-51 1.766339301523793 1102 52-53 1.8881480104953816 1102 54-55 1.5793214987937993 1102 56-57 1.6103729181884034 1102 58-59 1.668371028566309 1102 60-61 1.5625036042940543 1102 62-63 1.5669653259768914 1102 64-65 1.7010623661981832 1102 66-67 1.4104294771532153 1102 68-69 1.3656767164439039 1102 70-71 1.1269402228283667 1102 72-73 0.9364499505747723 1102 74-75 0.9107689806088111 1102 76-77 0.8287460712288599 1102 78-79 1.2564504223510902 1102 80-81 1.1606583461474465 1102 82-83 1.3110838319469593 1102 84-85 0.7644832342754064 1102 86-87 0.4513505802220088 1102 88-89 0.932909108262514 1102 90-91 1.0595706834142788 1102 92-93 1.255837361994658 1102 94-95 1.6419697729877427 1102 96-97 1.4804602386948904 1102 98-99 0.9817280262397325 1102 100-101 0.6428432954095324 1103 1 0.33123372523204253 1103 2 0.33335066313823347 1103 3 0.43046715604832286 1103 4 0.16526161855726684 1103 5 0.1827696467891471 1103 6 0.27863604808130304 1103 7 0.22247304924333378 1103 8 0.28773858292711907 1103 9 0.1273162919626074 1103 10-11 0.29419607435212214 1103 12-13 0.20623352396447103 1103 14-15 0.42945030716778376 1103 16-17 0.30678275713324865 1103 18-19 0.2641098862333848 1103 20-21 0.11100362537590058 1103 22-23 0.10696541355684985 1103 24-25 0.2725165684274131 1103 26-27 -0.0031435262808443554 1103 28-29 0.11762009435783227 1103 30-31 0.4864971251730026 1103 32-33 0.6501815885387856 1103 34-35 0.4737437404359284 1103 36-37 0.7950490954275438 1103 38-39 0.10885169415699636 1103 40-41 0.199230212461039 1103 42-43 0.4466198577861178 1103 44-45 0.465332703543325 1103 46-47 0.7329411624903521 1103 48-49 0.20659234784172753 1103 50-51 0.4355469002645407 1103 52-53 0.6026043044881355 1103 54-55 0.6356313542169154 1103 56-57 0.43455267881900994 1103 58-59 0.049105557235904485 1103 60-61 0.05285526743374547 1103 62-63 0.4063810917253825 1103 64-65 0.5529773911077989 1103 66-67 0.020343818747917908 1103 68-69 0.14803830159505438 1103 70-71 0.6951279043338943 1103 72-73 0.8109631758560276 1103 74-75 0.32431221247917463 1103 76-77 0.578441019927947 1103 78-79 0.3566039938917207 1103 80-81 0.724248468760635 1103 82-83 0.5390033472117253 1103 84-85 0.9120522368796742 1103 86-87 1.1455532109844277 1103 88-89 1.2639577526145338 1103 90-91 1.3273865509627427 1103 92-93 0.9260254827740582 1103 94-95 1.3554211408377768 1103 96-97 1.094242630785173 1103 98-99 1.607542513213172 1103 100-101 0.7120550777109784 1104 1 0.16169605640881457 1104 2 0.1681970759491307 1104 3 0.17560948534878307 1104 4 0.24135159506725046 1104 5 0.17478087581140755 1104 6 -0.04186377330796631 1104 7 0.20274468188976158 1104 8 0.13900455415455326 1104 9 0.15567390312523344 1104 10-11 0.24135706576797844 1104 12-13 0.18914282218666045 1104 14-15 0.2779072186815057 1104 16-17 0.1485661221594654 1104 18-19 0.09606515055922671 1104 20-21 0.26365175501481986 1104 22-23 0.15843777940936832 1104 24-25 0.2597585300423404 1104 26-27 0.3318219603522081 1104 28-29 0.24086059822052164 1104 30-31 0.03250562632224785 1104 32-33 0.33442126610130885 1104 34-35 0.36292660535208654 1104 36-37 0.00627072067696588 1104 38-39 0.15407629264367984 1104 40-41 0.2610710215856997 1104 42-43 0.042539233983625024 1104 44-45 0.1249628891180734 1104 46-47 0.13994270323344438 1104 48-49 0.1979973828602546 1104 50-51 0.2630594481195878 1104 52-53 0.28981541515823395 1104 54-55 -0.027695532291300395 1104 56-57 0.15612109755473114 1104 58-59 0.47510207883697575 1104 60-61 0.20659400503473435 1104 62-63 0.1993784684929949 1104 64-65 0.4775702849264256 1104 66-67 0.6536794373364252 1104 68-69 0.2709775126046914 1104 70-71 0.5798671507048461 1104 72-73 0.7269033719309235 1104 74-75 0.754942690326029 1104 76-77 0.6033427840246652 1104 78-79 0.8945263000269108 1104 80-81 0.9733094092994996 1104 82-83 0.9071823115581346 1104 84-85 0.3908363488235018 1104 86-87 0.5718230611318269 1104 88-89 0.7814092841564673 1104 90-91 0.7793059848930106 1104 92-93 0.5200405798913863 1104 94-95 0.5396292457959397 1104 96-97 0.487126707006853 1104 98-99 -0.09254210136651508 1104 100-101 0.2895125990269971 1105 1 0.012757101044595487 1105 2 0.24689137341036727 1105 3 0.1712214988446057 1105 4 0.37875825816944086 1105 5 0.3298150762944516 1105 6 0.2425164696906137 1105 7 0.24277860581904065 1105 8 0.2721449144119319 1105 9 0.304994502693809 1105 10-11 0.37312295378213634 1105 12-13 0.08925897477059408 1105 14-15 0.13696435469064738 1105 16-17 0.3219063386088834 1105 18-19 0.024741932948280976 1105 20-21 -0.14370826268462622 1105 22-23 -0.2574622187469373 1105 24-25 -0.05518551391127602 1105 26-27 0.009673690656974543 1105 28-29 0.06651649158138184 1105 30-31 -0.08430239004972861 1105 32-33 -0.07847535809899142 1105 34-35 -0.015477310649309572 1105 36-37 0.28911424655317575 1105 38-39 0.31142248265455663 1105 40-41 0.25090306126194406 1105 42-43 0.1803137136016133 1105 44-45 0.37865856934816833 1105 46-47 -0.2291892866631251 1105 48-49 0.1688403187550911 1105 50-51 0.5291401280731272 1105 52-53 0.47500872624435075 1105 54-55 0.6358002917467509 1105 56-57 0.6014501043579585 1105 58-59 0.22775699504118307 1105 60-61 0.23488545583581555 1105 62-63 0.6509714182155193 1105 64-65 0.5728679489689021 1105 66-67 -0.031211508293104373 1105 68-69 -0.09123049270514016 1105 70-71 0.2742512654562219 1105 72-73 0.3768065781109762 1105 74-75 0.3646478840058549 1105 76-77 0.3085926964493062 1105 78-79 0.03163612874794097 1105 80-81 0.2533975377679454 1105 82-83 0.49375746965471023 1105 84-85 0.38067576326698216 1105 86-87 0.5524006897754212 1105 88-89 0.6673658836512963 1105 90-91 0.5310021813568113 1105 92-93 0.2345421378109691 1105 94-95 0.04120468044628112 1105 96-97 -0.04566335180691716 1105 98-99 -0.36693506583857527 1105 100-101 -0.221591034889558 1106 1 0.04251770936312127 1106 2 0.17502228416866572 1106 3 0.07462418507528668 1106 4 0.27149626470351507 1106 5 0.22547813202513822 1106 6 0.09090492233767833 1106 7 0.13224193284756325 1106 8 0.0433071008432222 1106 9 0.21286787156333986 1106 10-11 0.007574913049253951 1106 12-13 0.019923272357573296 1106 14-15 0.1875633115313704 1106 16-17 0.06778050990862994 1106 18-19 -0.0045372172399069655 1106 20-21 -0.3698846826995279 1106 22-23 -0.06780210526038388 1106 24-25 -0.05309824007240849 1106 26-27 0.11726620895047546 1106 28-29 0.45628264048950484 1106 30-31 0.33091608112965076 1106 32-33 -0.08096098122426554 1106 34-35 0.0735685616114381 1106 36-37 0.20982936472057645 1106 38-39 0.16903506170348237 1106 40-41 0.017911716044999082 1106 42-43 0.14037848118343277 1106 44-45 0.11296246113472108 1106 46-47 0.2913615243645573 1106 48-49 0.17279610867121775 1106 50-51 -0.22093247275605066 1106 52-53 -0.003933212197786418 1106 54-55 -0.12434280830865418 1106 56-57 -0.31294396530062585 1106 58-59 -0.5948056231739827 1106 60-61 -0.2044164298751916 1106 62-63 -0.19068330752529405 1106 64-65 -0.03618900447103712 1106 66-67 -0.47036628179762374 1106 68-69 -0.7817751899787915 1106 70-71 -0.9364497146549695 1106 72-73 -0.4190398902407928 1106 74-75 -0.3550021035755826 1106 76-77 -0.6795513970911351 1106 78-79 -0.6428790696624809 1106 80-81 -0.2708626024662877 1106 82-83 -0.8237804069620935 1106 84-85 -0.39972774449939763 1106 86-87 -0.3575446685478809 1106 88-89 -0.3050668172906015 1106 90-91 -0.06730984936480411 1106 92-93 -0.3269994834801828 1106 94-95 -0.9808726585422747 1106 96-97 -0.0601166484577238 1106 98-99 -0.09200336793444563 1106 100-101 0.1560240278719487 1107 1 0.040902470987834505 1107 2 0.18856088142296556 1107 3 0.14123694239541962 1107 4 0.3090857817860453 1107 5 -0.17249945067159445 1107 6 0.09011670234670532 1107 7 -0.17990135166105148 1107 8 0.08320410131901212 1107 9 -0.07991093062815935 1107 10-11 -0.013925522922164646 1107 12-13 0.0617734656348361 1107 14-15 0.21857650094307957 1107 16-17 0.2238337739766223 1107 18-19 0.06718161284558732 1107 20-21 -0.007197301774859 1107 22-23 -0.0641570983073052 1107 24-25 -0.019670361180565976 1107 26-27 0.1124735863628672 1107 28-29 0.3795843429221577 1107 30-31 0.2622960174594482 1107 32-33 -0.24704873502191305 1107 34-35 0.3228216925396836 1107 36-37 0.26018967927345216 1107 38-39 0.36601042063967526 1107 40-41 0.4882523863587167 1107 42-43 0.4109390771267556 1107 44-45 0.3712035461828336 1107 46-47 0.2511623048248701 1107 48-49 0.5409887774084972 1107 50-51 0.27328962966762305 1107 52-53 0.16637437738055283 1107 54-55 0.44326032905195234 1107 56-57 -0.10039309345988912 1107 58-59 0.005655669302601751 1107 60-61 0.07523524223345746 1107 62-63 -0.031191280595127324 1107 64-65 -0.04083970661985603 1107 66-67 0.20839016843527247 1107 68-69 0.16153831533352658 1107 70-71 -0.07121695492525149 1107 72-73 -0.19062558057651913 1107 74-75 -0.190142625230191 1107 76-77 -0.3409700705536416 1107 78-79 -0.30269067283251516 1107 80-81 -0.23461265096428008 1107 82-83 -0.20961102951294563 1107 84-85 -0.24538773580038864 1107 86-87 0.6735011931950616 1107 88-89 0.01329339924527062 1107 90-91 -0.1472855901588659 1107 92-93 -0.16121088571537712 1107 94-95 -0.40929472788521437 1107 96-97 -0.3235168988094639 1107 98-99 0.12742502963064872 1107 100-101 0.03426548048379452 1108 1 -0.11385338659468758 1108 2 0.12608782694285736 1108 3 -0.2214060022536799 1108 4 0.021962858992161216 1108 5 -0.006316558355493385 1108 6 0.08384399874528725 1108 7 0.04439208878561374 1108 8 0.014870262868342365 1108 9 0.07395492443589546 1108 10-11 -0.20170431567749603 1108 12-13 0.02552240382127735 1108 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58-59 -2.933743192326098 2316 60-61 -2.589468127042977 2316 62-63 -2.466293435846186 2316 64-65 -2.3552891897245196 2316 66-67 -2.555635874367134 2316 68-69 -2.2294908915892684 2316 70-71 -2.6385646565989873 2316 72-73 -2.4563449395640014 2316 74-75 -2.1917246854241306 2316 76-77 -1.5590073368604358 2316 78-79 -1.628192326096542 2316 80-81 -1.629044066095986 2316 82-83 -1.8639787172129303 2316 84-85 -1.750906032960689 2316 86-87 -1.5524063219246251 2316 88-89 -2.0450336666672264 2316 90-91 -2.08482202649596 2316 92-93 -2.1785423440144065 2316 94-95 -1.7389239784203543 2316 96-97 -1.2419618070677725 2316 98-99 -0.7333047206664052 2316 100-101 -0.655481612397157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 16.0 4 32.0 5 101.0 6 272.0 7 534.0 8 1086.0 9 1826.0 10 2701.0 11 3758.0 12 5130.0 13 6684.0 14 8494.0 15 10459.0 16 12895.0 17 14757.0 18 12972.0 19 10354.0 20 10325.0 21 11015.0 22 11912.0 23 12764.0 24 14124.0 25 16290.0 26 18561.0 27 21707.0 28 24650.0 29 28031.0 30 31384.0 31 35433.0 32 40057.0 33 45736.0 34 51095.0 35 50895.0 36 40842.0 37 22777.0 38 4052.0 39 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.908188946968956 21.80812332848476 15.90485833774502 18.37882938680126 2 18.274936955210787 25.067327723260785 35.259511539937506 21.39822378159092 3 21.980134242904963 28.363867483956295 30.213562028239664 19.442436244899078 4 14.618565672623616 19.85048240324526 34.7750041113913 30.75594781273983 5 12.64313271340565 40.71050702044117 31.76261383804675 14.883746428106434 6 29.18884379705592 37.81586886099629 16.019953849869893 16.975333492077905 7 26.02771406105727 37.121218610117054 19.640357894772905 17.210709434052767 8 27.48399961626489 33.05398330752258 19.016644510532156 20.445372565680376 9 27.638343323391812 17.277861813422597 19.732521109316192 35.3512737538694 10-11 25.317132250786727 28.128367254992316 24.547278187296897 22.007222306924064 12-13 28.123656310011015 26.235852175685697 26.16253274106762 19.477958773235667 14-15 24.580681633247394 26.50446684768178 27.27800666386864 21.636844855202185 16-17 20.282563111586217 30.238398706246745 27.998057314365592 21.480980867801442 18-19 20.22667761909697 26.945890825740726 32.95159716755762 19.875834387604684 20-21 21.747859715542383 25.755191715525257 32.82705987657229 19.669888692360075 22-23 21.0797703447313 25.83202068713281 32.91011735442475 20.178091613711135 24-25 21.194510278880095 26.562431744655846 33.0307471320978 19.21231084436625 26-27 21.0744552006263 26.240391813576775 33.44094273717894 19.24421024861798 28-29 20.212575514500756 26.457420228507118 34.04865647786578 19.28134777912635 30-31 20.02797461556317 27.17083787656736 33.27925707095668 19.521930436912786 32-33 19.78620160948388 27.591384678638576 32.950906030639295 19.67150768123825 34-35 20.758967383623375 26.669641732927555 32.728412710785015 19.842978172664054 36-37 21.455644322015825 26.625997980270377 32.41289166523196 19.505466032481845 38-39 21.144853708949466 26.426013312969566 33.11607604033808 19.313056937742896 40-41 20.51419536203184 26.882622094845882 33.02106905902728 19.582113484094993 42-43 20.43020976002097 27.78975517290705 31.991294046906276 19.788741020165705 44-45 20.486653309840396 27.77249081968027 31.070638994794606 20.670216875684723 46-47 20.831113478955782 28.2786892266684 30.082552886781432 20.807644407594385 48-49 21.527012836172208 28.059019325351482 29.452102901800625 20.961864936675685 50-51 21.497425232380056 28.715768267488034 28.98592186274073 20.800884637391174 52-53 21.44167648832365 28.786677036509158 29.14479634908453 20.626850126082665 54-55 20.53367389241211 28.607219578378267 29.30598239645325 21.55312413275637 56-57 21.34562060396424 29.181925102815093 27.974185081725256 21.498269211495415 58-59 21.813944245630324 28.165241657658648 28.3843464287978 21.63646766791323 60-61 21.36339380317593 28.365721059831138 28.990919673241862 21.279965463751072 62-63 22.220880314383308 28.07137008219328 29.115317278405335 20.592432325018073 64-65 22.06024817747079 28.283443443134775 29.04937253259396 20.60693584680048 66-67 21.519456814360467 28.697719795491665 28.896951818570376 20.88587157157749 68-69 21.413661402046632 28.62989398524513 28.921034125667354 21.03541048704088 70-71 21.47116717232542 28.955059471293943 28.283084387050796 21.290688969329842 72-73 21.593010556971244 28.440095076982374 28.073920212424248 21.892974153622134 74-75 21.3585579936767 29.24597555825665 27.837324582479962 21.558141865586684 76-77 21.51032217945226 28.528136525834142 28.694476850257733 21.267064444455865 78-79 21.140628586895815 28.353222938316524 28.982203857315643 21.523944617472015 80-81 20.730651257966954 29.645758082158224 28.298324852095842 21.325265807778983 82-83 20.94116659500148 29.164189792250912 28.084586617511775 21.810056995235836 84-85 20.855385948968415 28.548583136398015 27.930496037167458 22.66553487746612 86-87 21.500159744545773 28.474872140122947 27.811825207774977 22.213142907556303 88-89 21.45256105301297 28.111924171005825 28.134365880378837 22.301148895602367 90-91 21.220941437880057 28.28348153674705 28.2446782539722 22.250898771400696 92-93 20.785633830564514 28.81139905693104 28.031247189423237 22.37171992308121 94-95 20.59478025507713 28.69377896170417 27.519207873300843 23.19223290991786 96-97 20.79526026730804 28.36798881705399 27.12969339404467 23.707057521593295 98-99 21.145796305734123 28.167899373439486 27.040004796635504 23.64629952419089 100-101 21.420085500467245 28.396399421317213 26.73547018832887 23.44804488988667 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3517.0 1 2417.0 2 1811.0 3 2251.5 4 1861.0 5 1701.0 6 2533.5 7 4262.0 8 5558.5 9 5777.0 10 4695.0 11 3273.5 12 2836.0 13 2657.5 14 2497.0 15 2333.5 16 2163.5 17 2080.0 18 2004.0 19 1951.0 20 1961.0 21 2010.0 22 2093.0 23 2195.0 24 2385.0 25 2655.5 26 3003.5 27 3425.0 28 3885.5 29 4527.5 30 5385.5 31 6361.5 32 7774.0 33 9053.0 34 9975.5 35 10729.0 36 11573.0 37 12469.5 38 13109.5 39 13408.5 40 13539.5 41 14117.5 42 14619.0 43 15424.0 44 16390.5 45 17057.5 46 17647.5 47 20082.5 48 28066.5 49 29126.0 50 22886.0 51 19296.0 52 16476.5 53 15685.5 54 15458.0 55 14836.0 56 14063.5 57 13086.0 58 12408.0 59 11744.5 60 10434.5 61 8986.0 62 7723.0 63 6303.5 64 4984.5 65 4088.0 66 3254.0 67 2637.0 68 1982.0 69 1362.5 70 1060.5 71 902.0 72 735.5 73 630.0 74 588.0 75 504.5 76 364.5 77 245.5 78 177.0 79 122.0 80 90.5 81 61.0 82 36.0 83 22.0 84 14.5 85 9.0 86 6.0 87 3.5 88 3.0 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011991496316041026 2 0.005995748158020513 3 0.004967905616645567 4 5.139212706874725E-4 5 0.003940063075270623 6 0.0 7 0.0 8 0.0018843779925207325 9 0.0 10-11 0.0 12-13 0.0 14-15 3.42614180458315E-4 16-17 0.003254834714353992 18-19 0.0017987244474061538 20-21 7.708819060312087E-4 22-23 4.282677255728938E-4 24-25 2.5696063534373626E-4 26-27 0.0 28-29 2.5696063534373626E-4 30-31 5.995748158020512E-4 32-33 0.003340488259468571 34-35 0.0011134960864895237 36-37 9.421889962603662E-4 38-39 0.0052248662519893034 40-41 0.0 42-43 0.0014561102669478389 44-45 0.004368330800843516 46-47 0.0 48-49 0.001027842541374945 50-51 8.565354511457876E-4 52-53 5.139212706874725E-4 54-55 1.713070902291575E-4 56-57 0.009164929327259926 58-59 0.00137045672183326 60-61 0.0021413386278644688 62-63 1.713070902291575E-4 64-65 0.0019700315376353114 66-67 9.421889962603662E-4 68-69 0.001284803176718681 70-71 0.0034261418045831503 72-73 0.001627417357176996 74-75 0.005567480432447619 76-77 6.8522836091663E-4 78-79 0.0042826772557289375 80-81 0.001284803176718681 82-83 0.002398299263208205 84-85 0.0017987244474061538 86-87 4.282677255728938E-4 88-89 0.0021413386278644688 90-91 0.005738787522676776 92-93 0.0024839528083227835 94-95 3.42614180458315E-4 96-97 1.713070902291575E-4 98-99 7.708819060312087E-4 100-101 0.001284803176718681 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 583747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.78625229292304 #Duplication Level Percentage of deduplicated Percentage of total 1 74.05594454830633 32.4263227178286 2 12.792300551479816 11.202537987079873 3 4.951998315169182 6.504883427463828 4 2.448271767265906 4.288025811325821 5 1.4000024184780733 3.065042955309167 6 0.8763439159589003 2.3023089479726733 7 0.6055952566612766 1.8561722686897697 8 0.3908441345232983 1.3690879905157034 9 0.31762281814359816 1.2516761564302323 >10 1.9354224001519784 15.956714316462438 >50 0.14795045302119814 4.495902294414486 >100 0.06593017525859328 5.3619528758297355 >500 0.005494181271549439 1.6829562384911048 >1k 0.004709298232756663 3.665937144370965 >5k 0.001569766077585554 4.570478867815642 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8112 1.3896431159389255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7494 1.2837753341773064 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5547 0.9502404295011366 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5445 0.9327671062977626 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3584 0.6139646113813004 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2671 0.45756123800207965 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2077 0.3558048264059601 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2016 0.3453550939019815 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1951 0.33422013303708625 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1702 0.2915646675700261 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1490 0.25524756444144464 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1426 0.24428391066677857 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1232 0.21105033516232202 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1078 0.18466904326703176 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1071 0.18346989363542768 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1036 0.17747414547740717 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 957 0.16394088534930373 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 854 0.1462962550557005 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 851 0.14578233378501304 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 808 0.13841612890515925 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 781 0.133790837468972 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 773 0.13242038074713874 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 742 0.12710986095003488 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 647 0.1108356873782649 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 619 0.1060390888518485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.5696063534373626E-4 0.0 22-23 0.0 0.0 0.0 5.139212706874725E-4 0.0 24-25 0.0 0.0 0.0 0.0014561102669478389 0.0 26-27 0.0 0.0 0.0 0.00205568508274989 0.0 28-29 0.0 0.0 0.0 0.003340488259468571 0.0 30-31 0.0 0.0 0.0 0.009250582872374504 0.0 32-33 0.0 0.0 0.0 0.01627417357176996 0.0 34-35 0.0 0.0 0.0 0.026124331259946516 0.0 36-37 0.0 0.0 0.0 0.041285008745226955 0.0 38-39 0.0 0.0 0.0 0.06201316666295501 0.0 40-41 0.0 0.0 0.0 0.08865141919358902 0.0 42-43 0.0 0.0 0.0 0.11948669543483735 0.0 44-45 0.0 0.0 0.0 0.15263461739417933 0.0 46-47 0.0 0.0 0.0 0.1963179254026145 0.0 48-49 0.0 0.0 0.0 0.23520463488463322 0.0 50-51 0.0 0.0 0.0 0.2794018641637559 0.0 52-53 0.0 0.0 0.0 0.3246269359842534 0.0 54-55 0.0 0.0 0.0 0.36899547235360525 0.0 56-57 0.0 0.0 0.0 0.42946687520449783 0.0 58-59 0.0 0.0 0.0 0.501415853100744 0.0 60-61 0.0 0.0 0.0 0.5892963903883017 0.0 62-63 0.0 0.0 0.0 0.6690398408899746 0.0 64-65 0.0 0.0 0.0 0.7479267559405016 0.0 66-67 0.0 0.0 0.0 0.8382055924912677 0.0 68-69 0.0 0.0 0.0 0.9438164136175432 0.0 70-71 0.0 0.0 0.0 1.06647229022162 0.0 72-73 0.0 0.0 0.0 1.1975222142469255 0.0 74-75 0.0 0.0 0.0 1.3370518392385742 0.0 76-77 0.0 0.0 0.0 1.480435873760379 0.0 78-79 0.0 0.0 0.0 1.6344409478763917 0.0 80-81 0.0 0.0 0.0 1.8183391092373922 0.0 82-83 0.0 0.0 0.0 2.0102030502940487 0.0 84-85 0.0 0.0 0.0 2.2185981255578184 0.0 86-87 0.0 0.0 0.0 2.42981976781037 0.0 88-89 0.0 0.0 0.0 2.6484932684878895 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTAAGG 175 3.6379788E-12 32.57263 3 GTAAGGT 230 0.0 28.914114 4 GTAAGCG 50 4.95482E-5 28.498611 94-95 TAAGGTG 220 7.6397555E-11 25.907831 5 ACTTGAC 150 1.0590647E-6 25.3321 8 GAGTGCA 295 0.0 24.151365 9 AGGTAAG 140 1.777026E-5 23.75088 2 GGGATAG 100 0.0016531429 23.748844 7 ATGGGAG 805 0.0 23.601336 5 ATCCCGA 125 2.2547998E-4 22.79889 6 TCCCGAT 125 2.2547998E-4 22.79889 7 GCAATAG 105 0.002193371 22.621822 1 AGGTGTG 295 5.456968E-12 22.541275 7 GAGTACT 6280 0.0 22.42526 12-13 TGTAGGA 300 7.2759576E-12 22.167486 2 GTGTAGG 215 4.40632E-8 22.095734 1 CGTATAG 65 2.942516E-4 21.922009 14-15 AGTACTT 6385 0.0 21.907703 12-13 TGGGAGT 285 8.367351E-11 21.665611 6 CCCTACA 155 3.901072E-5 21.452406 2 >>END_MODULE