FastQCFastQC Report
Thu 26 May 2016
SRR936390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585068
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100551.7186036494903154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76711.3111296464684448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52130.8910075410037808No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41860.715472389534208No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29980.5124190692363965No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.4590919346127288No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23640.4040555969562512No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23090.394654980275797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.32150109047153497No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16380.2799674567742553No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15690.26817395584786724No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG14030.23980118550322355No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13720.23450265610151297No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT13080.2235637566915299No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12330.21074473394545593No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.18903785542877066No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC10600.1811755214778453No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9110.15570839628897837No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8230.1406674096002516No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8170.13964188778056566No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG8130.13895820656744173No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8000.13673624262478892No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.13280507564932623No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG6990.11947329199340931No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG6480.11075635652607901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG353.4046352E-667.89168
ACGCATA407.5181943E-659.405157
ATGGGCG702.0429087E-433.9545145
CTAGCGG300.003954394331.6610828-29
AACGCAT804.4753944E-429.7051166
TCGTAGC650.0058272829.2456137
ACAGTAC856.3748274E-427.9625423
CCAAGAC2700.026.4090673
GAGTACT48000.025.34619912-13
CGGTTAC1051.2678356E-924.87656428-29
AATAGCG1351.3333392E-524.6484645
ATGGGAG9750.024.37765
CAAGACT2551.8189894E-1124.2342034
TAAGGTG1401.7670563E-523.768165
GTACAAT1000.001645340623.7681581
ATGTGCA2602.3646862E-1123.762069
GTATCAA100500.023.508011
GTACATG61250.023.0502621
CTATGTC3100.023.0014463
GTACTTT52850.022.88335614-15
Produced by FastQC (version 0.11.5)