FastQCFastQC Report
Thu 26 May 2016
SRR936390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585068
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86121.4719656518558528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78691.3449718665180799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59091.0099680720873472No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55190.9433091538077625No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34570.5908714884423691No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25610.4377268967026055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.3896982914806484No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22020.37636650782473147No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20200.345259012627592No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.30765654590577507No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.2722760431266109No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.2394593448966616No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13180.22527295972433972No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT9660.16510901296943262No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG9540.16305796933006078No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.1627161287234988No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC9320.1592977226578791No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA8730.14921342476430088No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7970.13622348171494597No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT7850.13417243807557414No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA7480.12784838685417765No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG7400.12648102442792974No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG7230.12357537927215297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG2300.035.105355
GTAAGGT2550.029.8036274
ATAGCCC700.00838866527.142593
AAGGTGT2650.026.884236
GTGTAAG908.454189E-426.6437241
CCGCCTA458.948158E-426.38862874-75
AGGTAAG2059.567884E-1025.4831942
GGTAAGG2651.8189894E-1225.0940913
AGGTGTG3250.024.8437847
ACAGTAC1151.2815311E-424.7802478
GAGTACT67900.023.21914712-13
AGTACTT67750.022.67477412-13
ATATAGG1503.0341624E-522.1626592
TAGATCT2806.548362E-1122.0533544
AGGACCG1302.9410145E-421.9209865
GTACTTT72200.021.83628814-15
CATGGGT4150.021.7467734
AGGCTTA1754.0469677E-621.7122156
ATAGATC2858.367351E-1121.666453
GTATATG1100.002875860821.5906943
Produced by FastQC (version 0.11.5)