##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936390_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 585068 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.668303513437753 31.0 31.0 33.0 28.0 34.0 2 30.829202759337377 31.0 31.0 34.0 27.0 34.0 3 30.992370117661537 31.0 31.0 34.0 27.0 34.0 4 34.94596012771165 35.0 35.0 37.0 32.0 37.0 5 34.69755481414126 35.0 35.0 37.0 32.0 37.0 6 34.91189229286169 35.0 35.0 37.0 32.0 37.0 7 34.81942099038061 35.0 35.0 37.0 32.0 37.0 8 34.823818769784026 35.0 35.0 37.0 32.0 37.0 9 36.23637936103154 38.0 35.0 39.0 32.0 39.0 10-11 36.08779321378028 37.5 35.0 39.0 31.0 39.0 12-13 35.89763753956805 37.0 35.0 39.0 30.0 39.0 14-15 36.59484880389972 38.0 35.0 40.0 30.5 41.0 16-17 36.48425909466934 38.0 35.0 40.0 30.5 41.0 18-19 36.65244552769934 38.5 35.0 40.0 30.5 41.0 20-21 36.77679004833627 38.5 35.0 40.0 31.0 41.0 22-23 36.630356642304825 38.0 35.0 40.0 30.0 41.0 24-25 36.40377101465129 38.0 35.0 40.0 30.0 41.0 26-27 35.97298604606644 38.0 34.5 40.0 29.5 41.0 28-29 35.68660993252067 38.0 34.0 40.0 28.5 41.0 30-31 35.450911688897705 38.0 34.0 40.0 27.5 41.0 32-33 35.04099010713284 37.0 33.5 40.0 27.0 41.0 34-35 34.69349972994592 37.0 33.0 40.0 26.0 41.0 36-37 34.282426999938465 37.0 33.0 40.0 24.5 41.0 38-39 33.91722586092557 36.5 33.0 40.0 24.0 41.0 40-41 33.46648936533873 36.0 32.5 39.0 22.5 40.5 42-43 33.012130213923854 36.0 31.5 39.0 21.0 40.0 44-45 32.48076890207634 35.0 31.0 39.0 18.5 40.0 46-47 31.738329561691973 35.0 30.5 39.0 12.5 40.0 48-49 30.987922771370165 35.0 29.5 39.0 7.5 40.0 50-51 30.648130644643015 35.0 28.5 38.5 6.0 40.0 52-53 30.207198137652377 35.0 28.0 38.0 2.0 40.0 54-55 29.79932332651931 34.0 27.0 38.0 2.0 40.0 56-57 29.45273540853371 34.0 27.0 38.0 2.0 40.0 58-59 28.97410044644383 34.0 26.0 38.0 2.0 40.0 60-61 28.457143443155324 33.0 26.0 37.0 2.0 39.5 62-63 27.966920939104515 33.0 25.0 36.5 2.0 39.0 64-65 27.355645839457978 33.0 23.0 36.0 2.0 39.0 66-67 26.804680823425652 32.0 22.0 35.5 2.0 38.5 68-69 26.267638975298595 31.5 21.5 35.0 2.0 38.0 70-71 25.65973015102518 31.0 20.0 35.0 2.0 37.5 72-73 25.119666431936118 31.0 18.5 34.5 2.0 37.0 74-75 24.664703931850656 30.5 18.0 34.0 2.0 36.0 76-77 23.656331400794436 29.5 16.0 33.5 2.0 35.0 78-79 24.34089712648786 30.5 16.5 34.0 2.0 35.0 80-81 24.120589401573834 31.0 12.0 34.0 2.0 35.0 82-83 23.659469497562675 30.5 7.5 34.0 2.0 35.0 84-85 22.99995470611963 30.0 3.5 34.0 2.0 35.0 86-87 22.37830474406394 29.0 2.0 34.0 2.0 35.0 88-89 21.674590816793945 29.0 2.0 34.0 2.0 35.0 90-91 20.910643720046217 28.0 2.0 33.0 2.0 35.0 92-93 20.14241250589675 27.0 2.0 33.0 2.0 35.0 94-95 19.25522246986675 25.5 2.0 33.0 2.0 34.0 96-97 18.17821261802047 24.5 2.0 32.5 2.0 34.0 98-99 16.896917965091237 19.0 2.0 32.0 2.0 34.0 100-101 14.373542049813015 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.33941789290133073 1101 2 0.3462226511441031 1101 3 0.2872846921275496 1101 4 0.08338681836139017 1101 5 0.5510908840727566 1101 6 0.20368914962570273 1101 7 0.2601032133249106 1101 8 0.29923876315890396 1101 9 0.4106263009797786 1101 10-11 0.313298842922471 1101 12-13 0.2627571311966008 1101 14-15 0.24698094558016237 1101 16-17 0.44196088129191935 1101 18-19 0.33452942126667295 1101 20-21 0.15889558785512747 1101 22-23 0.06100054189605686 1101 24-25 -0.030670659907279685 1101 26-27 0.13030822974121747 1101 28-29 0.03106825533738089 1101 30-31 -0.1356920305180509 1101 32-33 0.26971400494349496 1101 34-35 0.5834109886490921 1101 36-37 0.4397184215287169 1101 38-39 0.5634721399908216 1101 40-41 1.072100412591702 1101 42-43 0.9004173869790222 1101 44-45 1.0778027430196744 1101 46-47 1.3274963700401265 1101 48-49 1.1996059168097695 1101 50-51 1.3784636762754268 1101 52-53 1.361120930163434 1101 54-55 1.1947138447024876 1101 56-57 1.177490275211177 1101 58-59 1.1665641213996132 1101 60-61 1.1133153481758278 1101 62-63 0.9307801415419235 1101 64-65 1.120965048096334 1101 66-67 0.9386070828037383 1101 68-69 0.6798960065985824 1101 70-71 0.9094362139422749 1101 72-73 0.8352169108639025 1101 74-75 0.8919766709107506 1101 76-77 0.9444419782370446 1101 78-79 0.8309044321354655 1101 80-81 1.0206878776359538 1101 82-83 1.009157217033728 1101 84-85 0.9156580848469353 1101 86-87 0.9034174245634929 1101 88-89 1.0446129150232117 1101 90-91 0.883466815723299 1101 92-93 0.9305333618221745 1101 94-95 0.6757569840627475 1101 96-97 0.5421405920064579 1101 98-99 0.26440147359629407 1101 100-101 0.1628109444081094 1102 1 0.5651764043661593 1102 2 0.5952315560587387 1102 3 0.3583148333710504 1102 4 0.32719820151177714 1102 5 0.21236347008387213 1102 6 0.3893715609251842 1102 7 0.23043610174453732 1102 8 0.07505006662325542 1102 9 0.12935650397996312 1102 10-11 0.45980560627486255 1102 12-13 0.4759043312284774 1102 14-15 0.47065765932799764 1102 16-17 0.7441343179514917 1102 18-19 0.3553464023611568 1102 20-21 0.2574552627360944 1102 22-23 0.4212202761431243 1102 24-25 0.49917972955822876 1102 26-27 0.06692197159830471 1102 28-29 0.13051543345559224 1102 30-31 -0.032369122774561276 1102 32-33 0.394280214079501 1102 34-35 0.688429216830059 1102 36-37 0.5802616810860641 1102 38-39 0.7655170829157072 1102 40-41 0.9590548122570226 1102 42-43 0.3739656069921651 1102 44-45 0.3169206185899327 1102 46-47 0.5475702788394621 1102 48-49 0.5522285835029983 1102 50-51 0.36069468609673194 1102 52-53 0.011491470235547752 1102 54-55 0.0010080455511420894 1102 56-57 0.08957565879983775 1102 58-59 0.09921547506617046 1102 60-61 0.1869970423009768 1102 62-63 0.15146066029802085 1102 64-65 0.12167026952391069 1102 66-67 0.4198669190489319 1102 68-69 0.39265374112454765 1102 70-71 0.19269915794291137 1102 72-73 0.18036303513427754 1102 74-75 -0.4838137149688855 1102 76-77 -0.2280663389930666 1102 78-79 0.018762272245432143 1102 80-81 -0.2891772537463986 1102 82-83 0.19412345007554066 1102 84-85 0.5475554093882451 1102 86-87 0.4107067047993631 1102 88-89 0.33596498785625073 1102 90-91 0.42674629457661695 1102 92-93 0.28532348872218805 1102 94-95 0.2777461467617144 1102 96-97 0.3476847480317211 1102 98-99 0.062477055802403925 1102 100-101 0.056509771031215905 1103 1 0.4751469621762965 1103 2 0.4986691306588824 1103 3 0.24464410845659046 1103 4 0.16112037864235873 1103 5 -0.10027279951110302 1103 6 0.1499389198811798 1103 7 0.2275873709416416 1103 8 0.22994781469359538 1103 9 0.22479694322560562 1103 10-11 0.319899502988477 1103 12-13 0.21818769241252767 1103 14-15 0.4119105438668669 1103 16-17 0.3715951280095737 1103 18-19 0.28310760232933063 1103 20-21 0.3392050836957523 1103 22-23 0.3765636357357067 1103 24-25 0.5409836604884362 1103 26-27 0.29556046925088 1103 28-29 0.24698515574412738 1103 30-31 0.41697240662784196 1103 32-33 0.21577898069046597 1103 34-35 0.10297922876608823 1103 36-37 -0.14865930244222625 1103 38-39 -0.3339139666585851 1103 40-41 -0.2943131355427724 1103 42-43 0.06318538896081805 1103 44-45 0.33711239069146615 1103 46-47 0.44202201752013437 1103 48-49 0.5145127404219139 1103 50-51 0.09968967296710574 1103 52-53 0.2588436624850914 1103 54-55 0.4971944862291089 1103 56-57 0.5927824702902491 1103 58-59 0.11439013886819183 1103 60-61 0.19397165835890462 1103 62-63 0.1387289025197127 1103 64-65 0.41781095544736147 1103 66-67 0.1164293444487896 1103 68-69 0.140610691868563 1103 70-71 0.15242900595755415 1103 72-73 0.15680530457010278 1103 74-75 0.4881107697927938 1103 76-77 0.2989707422514627 1103 78-79 0.16629947285019142 1103 80-81 -0.14527457211992 1103 82-83 0.14890581659102153 1103 84-85 0.19844862176988087 1103 86-87 0.33829284309808116 1103 88-89 0.29030970019809743 1103 90-91 0.26116381665826083 1103 92-93 0.8565696095147395 1103 94-95 0.7983616476768098 1103 96-97 0.7137207948209294 1103 98-99 0.28000112678195066 1103 100-101 0.1383411548141389 1104 1 0.3632208007295681 1104 2 0.5028500885238394 1104 3 0.0682093556004375 1104 4 0.15272090939868832 1104 5 0.10495298055883495 1104 6 0.08796519065035824 1104 7 0.14435209400534177 1104 8 -0.03349425692512398 1104 9 0.023647805704634095 1104 10-11 0.06411417556356014 1104 12-13 0.13300067591401898 1104 14-15 0.1116712445319834 1104 16-17 -0.10520514571381057 1104 18-19 0.04235724139833508 1104 20-21 -0.10074058888768178 1104 22-23 0.01955281197852088 1104 24-25 -0.03353294233725279 1104 26-27 0.13110945989588174 1104 28-29 0.04277436369879695 1104 30-31 0.036992393442382365 1104 32-33 0.07854696673724959 1104 34-35 -0.12209775593711925 1104 36-37 -0.10807647190871705 1104 38-39 -0.0960833914221979 1104 40-41 0.14318425657432954 1104 42-43 0.37280680781601916 1104 44-45 0.6291514630362229 1104 46-47 0.4330728065611815 1104 48-49 0.5613179795543424 1104 50-51 0.5625993192130032 1104 52-53 0.4429069943997348 1104 54-55 0.6397820485053529 1104 56-57 0.7265209643108932 1104 58-59 0.5198512542910017 1104 60-61 0.4860774925058955 1104 62-63 0.23678947216479074 1104 64-65 0.18151590166238307 1104 66-67 0.3877284456680279 1104 68-69 0.06234812604559892 1104 70-71 0.1743300065500648 1104 72-73 0.12393554850103428 1104 74-75 -0.04212711085379439 1104 76-77 -0.2190601601177491 1104 78-79 0.14066711340464622 1104 80-81 0.23419220971085863 1104 82-83 0.5324064716912034 1104 84-85 0.6195413872940847 1104 86-87 0.7907824324295447 1104 88-89 0.5452041748244412 1104 90-91 0.33725662390859057 1104 92-93 0.4438126808914511 1104 94-95 0.33805675487122855 1104 96-97 0.5541590443264717 1104 98-99 1.4463266823653278 1104 100-101 0.7724687449634349 1105 1 0.5939441606901248 1105 2 0.6563615313455529 1105 3 0.3045988140023397 1105 4 0.2785192519226598 1105 5 0.24009648730273625 1105 6 0.22555930077368913 1105 7 0.3059338815761663 1105 8 0.321133127039964 1105 9 0.3527134789252102 1105 10-11 0.1898471967592812 1105 12-13 0.25253027806498807 1105 14-15 0.3666372458341982 1105 16-17 0.40688772857212996 1105 18-19 0.1711055793919556 1105 20-21 0.3080329083530202 1105 22-23 0.47681791332139767 1105 24-25 0.2505168535862694 1105 26-27 0.2408766841819343 1105 28-29 0.37598267341460456 1105 30-31 0.17997340600725664 1105 32-33 0.1928466483484499 1105 34-35 -0.0094932681511537 1105 36-37 0.10438114824565758 1105 38-39 -0.12112674581990746 1105 40-41 -0.09120236561764727 1105 42-43 -0.01960896454546912 1105 44-45 -0.06705800349924118 1105 46-47 0.43733238930539287 1105 48-49 0.12510636373646733 1105 50-51 0.3459843125860509 1105 52-53 0.23198231831977267 1105 54-55 0.3196243080333119 1105 56-57 0.25785939832687177 1105 58-59 0.18102214963569807 1105 60-61 0.4654865241277406 1105 62-63 0.7419551249461094 1105 64-65 0.3870115325083816 1105 66-67 0.14775263563087648 1105 68-69 -0.06563321312591697 1105 70-71 -0.01600370115173888 1105 72-73 0.28727469051592536 1105 74-75 0.024105555288894465 1105 76-77 -0.0748707029801281 1105 78-79 0.3448015320553637 1105 80-81 0.34666289413087625 1105 82-83 0.44688610302605625 1105 84-85 0.40097306223829676 1105 86-87 0.18323086545516531 1105 88-89 -0.13615733237038796 1105 90-91 0.13174518951543845 1105 92-93 0.41441070818734005 1105 94-95 0.7273623891518639 1105 96-97 0.5537344556928083 1105 98-99 0.7618803227650908 1105 100-101 0.5186564026038187 1106 1 0.34684154312772364 1106 2 0.31679168606957475 1106 3 0.09479287320770169 1106 4 -0.11498031336446246 1106 5 -0.28451543649167377 1106 6 0.05681051417128913 1106 7 -0.16348005793543763 1106 8 0.00899872901178611 1106 9 -0.03081934400736941 1106 10-11 -0.17615572383598277 1106 12-13 -0.046843397748936866 1106 14-15 -0.007271751456229936 1106 16-17 0.18365844496207018 1106 18-19 -0.03735507276176975 1106 20-21 -0.13242514396446126 1106 22-23 0.2570378442738033 1106 24-25 0.3078367009725085 1106 26-27 -0.07524820925010545 1106 28-29 0.29224557763427583 1106 30-31 0.02425017883390268 1106 32-33 0.2989535201883413 1106 34-35 -0.2782513398829991 1106 36-37 0.18337860749659995 1106 38-39 0.2836352594304117 1106 40-41 0.29627874758496375 1106 42-43 -0.06397922174355841 1106 44-45 -0.31099629115254857 1106 46-47 0.13946543676928513 1106 48-49 -0.15627420839632578 1106 50-51 -0.026075044714172435 1106 52-53 0.528697070702254 1106 54-55 0.020386743775997473 1106 56-57 -0.06412673306615346 1106 58-59 0.12753241814476013 1106 60-61 -0.05864426114098009 1106 62-63 -0.4946035950565886 1106 64-65 0.19615147629114205 1106 66-67 0.11265590146413373 1106 68-69 0.16175116590994776 1106 70-71 0.004545978511291793 1106 72-73 0.22985882645022215 1106 74-75 0.44308435987068506 1106 76-77 0.3428187347630782 1106 78-79 0.3246169267999761 1106 80-81 0.36024978183990086 1106 82-83 0.8084142243067269 1106 84-85 0.8791392913784115 1106 86-87 0.6450658180588 1106 88-89 0.7390610791971959 1106 90-91 0.3624701048941432 1106 92-93 0.7002914027150204 1106 94-95 0.6714268701553294 1106 96-97 0.649622409503511 1106 98-99 0.9923878817045626 1106 100-101 0.6651229887993626 1107 1 0.7545259142421088 1107 2 0.756720609605054 1107 3 0.6193609910923392 1107 4 0.4376744719511052 1107 5 0.40199634932653794 1107 6 0.46000190033871036 1107 7 0.3938793544050654 1107 8 0.582528918872363 1107 9 0.5841265549864474 1107 10-11 0.6594424736789861 1107 12-13 0.7821101203596825 1107 14-15 0.7384480079772686 1107 16-17 0.8808317004581454 1107 18-19 0.5784032231667666 1107 20-21 0.6242779574738293 1107 22-23 0.9029198626337518 1107 24-25 0.8131068091745206 1107 26-27 0.8703245030313127 1107 28-29 0.8063440264742141 1107 30-31 0.7341869948552144 1107 32-33 0.9108405297739637 1107 34-35 0.7670841569026905 1107 36-37 0.9152347085204156 1107 38-39 1.0747643699163945 1107 40-41 1.2384916418785679 1107 42-43 0.9352447573038773 1107 44-45 0.7442940994152707 1107 46-47 1.0569603000396874 1107 48-49 1.389851639274287 1107 50-51 1.227035652911031 1107 52-53 0.5419599742551924 1107 54-55 0.9620363614974927 1107 56-57 1.0188204269617174 1107 58-59 0.6551043103131207 1107 60-61 0.657625282682158 1107 62-63 0.5631228840128912 1107 64-65 0.6261684958415792 1107 66-67 0.5036833796605897 1107 68-69 0.8129870674611084 1107 70-71 0.879778564336565 1107 72-73 0.5712154521700263 1107 74-75 0.769374715164826 1107 76-77 0.744398554690946 1107 78-79 0.9046022000387204 1107 80-81 0.8016877574834247 1107 82-83 0.30884081537014296 1107 84-85 0.743876855201723 1107 86-87 1.0463314817771767 1107 88-89 1.1782646876946252 1107 90-91 1.1312445208942847 1107 92-93 1.1804411394851861 1107 94-95 1.4195011477062778 1107 96-97 1.2101939312982353 1107 98-99 1.3663329782221645 1107 100-101 1.2793325497302348 1108 1 0.40970897193988876 1108 2 0.45955992107081656 1108 3 0.018463427199389315 1108 4 0.045931963305847034 1108 5 0.11699449246410865 1108 6 -0.10390069396337509 1108 7 0.06736577037118252 1108 8 0.013091909916020938 1108 9 -0.040054134608851655 1108 10-11 -0.2755697526749614 1108 12-13 0.11212521641011364 1108 14-15 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-2.6010248303626824 2316 58-59 -2.4792023371966287 2316 60-61 -2.594891293703075 2316 62-63 -2.552574930492227 2316 64-65 -2.8693181761200144 2316 66-67 -2.4371837683087456 2316 68-69 -2.3077595168665113 2316 70-71 -2.0441820093000658 2316 72-73 -2.5804997463450157 2316 74-75 -2.730710063117659 2316 76-77 -2.9758398061432736 2316 78-79 -2.5477300951324864 2316 80-81 -3.0151576872455976 2316 82-83 -3.2404510628703953 2316 84-85 -3.452474109039688 2316 86-87 -2.9173611032465203 2316 88-89 -3.2939793488225035 2316 90-91 -3.0408888576541315 2316 92-93 -3.218737403577716 2316 94-95 -3.5979854885591926 2316 96-97 -3.407219527923413 2316 98-99 -4.021882394311454 2316 100-101 -4.199225237824313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 13.0 4 40.0 5 101.0 6 281.0 7 587.0 8 1133.0 9 1938.0 10 2836.0 11 3824.0 12 5095.0 13 6527.0 14 8115.0 15 10030.0 16 12030.0 17 13781.0 18 11742.0 19 9228.0 20 9098.0 21 9681.0 22 10387.0 23 11051.0 24 12800.0 25 14503.0 26 16872.0 27 19790.0 28 22745.0 29 25794.0 30 29459.0 31 33751.0 32 38364.0 33 44583.0 34 52133.0 35 56309.0 36 49458.0 37 33000.0 38 7871.0 39 112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64570027962854 22.10774329409328 16.134049090344185 18.112507335933994 2 18.1685475076151 25.36639647739743 35.314740300364775 21.150315714622696 3 21.660883685155117 28.635330313648407 30.326809674386805 19.376976326809675 4 14.717928030795107 20.154590891376138 34.652529451581806 30.47495162624695 5 12.589134938161035 40.70142274060451 31.729645101082266 14.979797220152188 6 28.943985998208753 38.153684699898136 15.94703521641929 16.955294085473827 7 25.831527275461998 37.249345375238434 19.718391708314247 17.20073564098532 8 27.5861951089446 33.095298324297346 18.911647876827992 20.40685868993006 9 27.603700183915276 17.084977061868003 19.788839282662053 35.522483471554665 10-11 25.286142197267836 28.241100385852913 24.641857563443534 21.830899853435714 12-13 27.990237007246183 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19.38431219051949 38-39 20.95927311013421 26.294037616140347 33.650362009202354 19.096327264523097 40-41 20.34028093517433 26.8648960936405 33.41147533698205 19.383347634203123 42-43 20.32435620208374 27.610533243305614 32.394338509927266 19.67077204468338 44-45 20.467050106400254 27.622702139115123 31.41772995701259 20.492517797472033 46-47 20.755578041301607 28.12847185426502 30.51386006953109 20.602090034902286 48-49 21.51348930547555 27.816019650643824 29.97321454516862 20.697276498712007 50-51 21.43151370956439 28.523790478339194 29.43026940550297 20.614426406593445 52-53 21.460680686976975 28.655889350763005 29.40197177706066 20.481458185199365 54-55 20.500436317700824 28.48534938321514 29.611826816487596 21.402387482596435 56-57 21.304240439418816 28.89510303044806 28.511970436487417 21.28868609364571 58-59 21.803463900468838 28.019632073810858 28.79561688228447 21.381287143435834 60-61 21.376248328084337 28.274070243960807 29.27820249302389 21.071478934930965 62-63 22.15175323071506 27.858117745793727 29.58566682933124 20.404462194159972 64-65 21.857496596011288 28.16588045140352 29.460806478220032 20.515816474365163 66-67 21.285445688402703 28.64498760789676 29.315186736176397 20.75437996752414 68-69 21.36317604378982 28.42261790306247 29.347639374943917 20.866566678203796 70-71 21.453385669711647 28.822183650778594 28.727578211863197 20.996852467646562 72-73 21.48143463665762 28.375761449951113 28.540186781707426 21.60261713168384 74-75 21.260349224363665 29.172591903847074 28.29183958104015 21.27521929074911 76-77 21.383026999587226 28.576421915874235 29.01637340840198 21.024177676136564 78-79 21.058029578880433 28.361811381646874 29.336748647890982 21.243410391581712 80-81 20.71389071465141 29.59060088907275 28.64144179872699 21.054066597548854 82-83 20.91359755211583 29.01443602082069 28.485371669843335 21.586594757220148 84-85 20.860736125533634 28.561380117858715 28.28002341790273 22.297860338704922 86-87 21.465457118828333 28.418476885944223 28.2035240302763 21.912541964951146 88-89 21.429853624511484 28.05666964136125 28.591174388905415 21.92230234522185 90-91 21.13948590302044 28.227637328446285 28.681694783126023 21.95118198540725 92-93 20.731540561282483 28.767479185646426 28.514937962885412 21.986042290185676 94-95 20.589874244260482 28.62562668476282 27.995395052844074 22.78910401813262 96-97 20.771233361423974 28.240409154699652 27.776486254104622 23.211871229771745 98-99 21.17128820848732 28.079242548041584 27.47515444074843 23.27431480272267 100-101 21.370506963645454 28.299879060345894 27.38911372173623 22.94050025427242 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3806.0 1 2620.5 2 2038.0 3 2551.0 4 2080.5 5 1817.5 6 2686.5 7 4768.5 8 6246.0 9 6313.0 10 5077.5 11 3428.0 12 2877.5 13 2708.5 14 2517.0 15 2362.5 16 2225.0 17 2046.5 18 1955.5 19 1944.0 20 1938.0 21 1983.0 22 2081.0 23 2279.5 24 2416.5 25 2614.5 26 2987.0 27 3456.0 28 3907.5 29 4589.5 30 5475.5 31 6436.5 32 7885.0 33 9219.0 34 10229.0 35 10937.5 36 11625.0 37 12392.0 38 12926.5 39 13233.0 40 13511.5 41 14219.0 42 14636.5 43 15279.0 44 16299.0 45 17095.5 46 17636.5 47 19855.0 48 28345.5 49 29000.0 50 21993.0 51 18605.0 52 15904.0 53 15358.5 54 15419.5 55 14943.5 56 13974.0 57 13030.5 58 12443.0 59 11852.5 60 10574.0 61 9031.0 62 7739.5 63 6226.5 64 4848.0 65 3978.5 66 3230.0 67 2632.0 68 1928.0 69 1315.0 70 1051.0 71 865.5 72 724.0 73 641.0 74 534.5 75 447.5 76 343.5 77 239.5 78 167.5 79 120.5 80 88.5 81 62.5 82 43.0 83 20.5 84 16.5 85 12.5 86 5.0 87 2.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9790314971934886 2 0.007862333950925362 3 0.0030765654590577504 4 0.006836812131239447 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0027347248524957784 10-11 8.546015164049308E-5 12-13 2.563804549214792E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.836812131239446E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004016627127103174 34-35 2.563804549214792E-4 36-37 0.0025638045492147924 38-39 0.0 40-41 0.0037602466721816954 42-43 0.010767979106702127 44-45 0.00222196394265282 46-47 0.0010255218196859167 48-49 0.00837509486076832 50-51 3.418406065619723E-4 52-53 0.0020510436393718335 54-55 0.00999883774193769 56-57 0.0038457068238221884 58-59 8.546015164049308E-4 60-61 0.006067670766475008 62-63 0.00333294591397923 64-65 0.015810128053491218 66-67 0.0030765654590577504 68-69 8.546015164049308E-5 70-71 0.00111098197132641 72-73 0.0 74-75 0.0 76-77 5.982210614834515E-4 78-79 0.009571536983735224 80-81 0.01307540320099544 82-83 0.012477182139511989 84-85 0.007776873799284869 86-87 0.009571536983735224 88-89 0.022903320639652145 90-91 0.02051043639371834 92-93 0.0025638045492147924 94-95 0.007691413647644377 96-97 0.009742457287016209 98-99 0.009229696377173252 100-101 0.011195279864904591 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 585068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.17461268237488 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29586721736082 32.81991158228067 2 12.784127893157715 11.294677963243746 3 4.817499196327887 6.3843348328631 4 2.473341717961457 4.370356496884282 5 1.382678358937035 3.053964048517262 6 0.8759658514607622 2.321727132675954 7 0.5684829564580375 1.7578760092645633 8 0.4095310730603107 1.4472701227069273 9 0.32167815554707285 1.2789007136705415 >10 1.8491485543505388 15.454162093810414 >50 0.14687696417775428 4.513194223841275 >100 0.06350383320679764 5.264245909651376 >500 0.006233505100053756 2.029595004986226 >1k 0.0035063466187802376 3.2065983323075864 >5k 0.001558376275013439 4.803185533296073 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8612 1.4719656518558528 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7869 1.3449718665180799 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5909 1.0099680720873472 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5519 0.9433091538077625 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3457 0.5908714884423691 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2561 0.4377268967026055 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2280 0.3896982914806484 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2202 0.37636650782473147 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2020 0.345259012627592 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1800 0.30765654590577507 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1593 0.2722760431266109 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1401 0.2394593448966616 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1318 0.22527295972433972 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 966 0.16510901296943262 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 954 0.16305796933006078 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.1627161287234988 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 932 0.1592977226578791 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 873 0.14921342476430088 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 797 0.13622348171494597 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 785 0.13417243807557414 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 748 0.12784838685417765 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 740 0.12648102442792974 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 723 0.12357537927215297 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.546015164049308E-5 0.0 22-23 0.0 0.0 0.0 5.127609098429585E-4 0.0 24-25 0.0 0.0 0.0 0.00111098197132641 0.0 26-27 0.0 0.0 0.0 0.0021365037910123265 0.0 28-29 0.0 0.0 0.0 0.0038457068238221884 0.0 30-31 0.0 0.0 0.0 0.007435033192722897 0.0 32-33 0.0 0.0 0.0 0.012477182139511989 0.0 34-35 0.0 0.0 0.0 0.020168595787156365 0.0 36-37 0.0 0.0 0.0 0.032645777926668354 0.0 38-39 0.0 0.0 0.0 0.05170339174249831 0.0 40-41 0.0 0.0 0.0 0.07503401314035292 0.0 42-43 0.0 0.0 0.0 0.0936643261979804 0.0 44-45 0.0 0.0 0.0 0.11750770850567797 0.0 46-47 0.0 0.0 0.0 0.1568193782603048 0.0 48-49 0.0 0.0 0.0 0.19408000437555978 0.0 50-51 0.0 0.0 0.0 0.23014418836784784 0.0 52-53 0.0 0.0 0.0 0.2683448761511482 0.0 54-55 0.0 0.0 0.0 0.30851114742218 0.0 56-57 0.0 0.0 0.0 0.36303472416881455 0.0 58-59 0.0 0.0 0.0 0.42183130849747374 0.0 60-61 0.0 0.0 0.0 0.49310507496564504 0.0 62-63 0.0 0.0 0.0 0.560533134609994 1.7092030328098615E-4 64-65 0.0 0.0 0.0 0.6285594153158265 1.7092030328098615E-4 66-67 0.0 0.0 0.0 0.7051317111857083 1.7092030328098615E-4 68-69 0.0 0.0 0.0 0.796232232834474 1.7092030328098615E-4 70-71 1.7092030328098615E-4 0.0 0.0 0.9046811652662596 1.7092030328098615E-4 72-73 1.7092030328098615E-4 0.0 0.0 1.0168048842185866 1.7092030328098615E-4 74-75 1.7092030328098615E-4 0.0 0.0 1.1398675025808966 1.7092030328098615E-4 76-77 1.7092030328098615E-4 0.0 0.0 1.2716470564105369 1.7092030328098615E-4 78-79 1.7092030328098615E-4 0.0 0.0 1.4158183322280489 1.7092030328098615E-4 80-81 1.7092030328098615E-4 0.0 0.0 1.573577772156399 1.7092030328098615E-4 82-83 1.7092030328098615E-4 0.0 0.0 1.7465491190767568 1.7092030328098615E-4 84-85 1.7092030328098615E-4 0.0 0.0 1.9432783881531719 1.7092030328098615E-4 86-87 1.7092030328098615E-4 0.0 0.0 2.1475281505739505 1.7092030328098615E-4 88-89 1.7092030328098615E-4 0.0 0.0 2.358101964216125 1.7092030328098615E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 230 0.0 35.10535 5 GTAAGGT 255 0.0 29.803627 4 ATAGCCC 70 0.008388665 27.14259 3 AAGGTGT 265 0.0 26.88423 6 GTGTAAG 90 8.454189E-4 26.643724 1 CCGCCTA 45 8.948158E-4 26.388628 74-75 AGGTAAG 205 9.567884E-10 25.483194 2 GGTAAGG 265 1.8189894E-12 25.094091 3 AGGTGTG 325 0.0 24.843784 7 ACAGTAC 115 1.2815311E-4 24.780247 8 GAGTACT 6790 0.0 23.219147 12-13 AGTACTT 6775 0.0 22.674774 12-13 ATATAGG 150 3.0341624E-5 22.162659 2 TAGATCT 280 6.548362E-11 22.053354 4 AGGACCG 130 2.9410145E-4 21.920986 5 GTACTTT 7220 0.0 21.836288 14-15 CATGGGT 415 0.0 21.746773 4 AGGCTTA 175 4.0469677E-6 21.712215 6 ATAGATC 285 8.367351E-11 21.66645 3 GTATATG 110 0.0028758608 21.590694 3 >>END_MODULE