FastQCFastQC Report
Thu 26 May 2016
SRR936389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590360
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101581.7206450301510943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77181.3073378955213768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51800.8774307202384986No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42440.7188833931838201No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30600.518327799986449No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.4490480384849922No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24380.412968358289857No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22710.38468053391151164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.33708245816112203No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16530.27999864489464055No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15500.2625516633918287No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.23172301646453014No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT13420.22731892404634463No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13220.22393116064774038No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12750.21596991666102042No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.19327190189037197No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC10070.17057388711972354No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9230.15634528084558574No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8560.14499627346026153No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG8350.14143912189172708No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8230.13940646385256453No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7930.13432481875465818No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.12992072633647267No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG6510.11027169862456805No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG6030.10214106646791789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG1054.6362402E-836.2035831
GTATAGT1551.3478711E-930.6562631
CTTGACA3350.028.368489
TAGATAG856.38991E-427.9512965
ACAGCGT908.9234766E-426.3984498
AAGACTT3800.026.2595085
CAAGACT4200.026.0235314
GTGTAGG2409.094947E-1225.7384851
GAGTACT52500.025.61186412-13
ATGGGTA2059.513315E-1025.4970365
ACTTGAC3350.024.1132098
TACATGG63750.023.926312
GTACATG63400.023.9084991
TAAGGGT1601.8497958E-623.7606184
CGGTTAC1800.023.74853928-29
GTACTTT56850.023.48297514-15
ATGGGAG10750.022.9850675
GCTTATG1050.002190349422.627241
TATAGTC1050.002190349422.627243
GACTTGA3800.022.5100577
Produced by FastQC (version 0.11.5)