##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936389_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 590360 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13100142286063 33.0 31.0 34.0 31.0 34.0 2 32.186257537773564 33.0 31.0 34.0 31.0 34.0 3 32.21472491361203 34.0 31.0 34.0 30.0 34.0 4 35.84207263364726 37.0 35.0 37.0 35.0 37.0 5 35.84463547665831 37.0 35.0 37.0 35.0 37.0 6 36.0104376990311 37.0 36.0 37.0 35.0 37.0 7 35.93256826343248 37.0 36.0 37.0 35.0 37.0 8 35.90260857781693 37.0 36.0 37.0 35.0 37.0 9 37.5101802290128 39.0 37.0 39.0 35.0 39.0 10-11 37.466207060098924 39.0 37.0 39.0 35.0 39.0 12-13 37.37557507283691 39.0 37.0 39.0 35.0 39.0 14-15 38.509305339115116 40.0 38.0 41.0 34.5 41.0 16-17 38.44232417507961 40.0 38.0 41.0 34.5 41.0 18-19 38.47274629039908 40.0 38.0 41.0 34.5 41.0 20-21 38.4305830340809 40.0 38.0 41.0 34.0 41.0 22-23 38.294408496510606 40.0 38.0 41.0 34.0 41.0 24-25 38.365879293990105 40.0 38.0 41.0 34.0 41.0 26-27 38.29742868758046 40.0 38.0 41.0 34.0 41.0 28-29 38.148317975472594 40.0 38.0 41.0 34.0 41.0 30-31 37.865678569008736 40.0 37.0 41.0 34.0 41.0 32-33 37.56537959888881 40.0 35.5 41.0 33.5 41.0 34-35 37.32144877701741 39.0 35.0 41.0 33.0 41.0 36-37 37.101731994037536 39.0 35.0 41.0 33.0 41.0 38-39 36.85261704722542 39.0 35.0 41.0 32.5 41.0 40-41 36.58721712175621 39.0 35.0 40.0 32.0 41.0 42-43 36.2962472050952 38.0 35.0 40.0 31.0 41.0 44-45 35.940941967612986 38.0 35.0 40.0 30.5 41.0 46-47 35.45490632834203 38.0 35.0 40.0 29.0 41.0 48-49 35.16315299139508 38.0 35.0 40.0 27.0 41.0 50-51 34.189303984009754 37.5 34.0 39.5 25.5 40.5 52-53 34.29627515414323 37.5 34.5 39.5 26.0 40.5 54-55 34.659255369604985 38.0 35.0 40.0 26.0 41.0 56-57 34.55336574293651 38.0 35.0 40.0 26.0 41.0 58-59 34.28781506199607 37.5 34.5 40.0 26.0 41.0 60-61 33.95331492648553 37.0 34.0 40.0 24.5 41.0 62-63 33.58923453486008 36.0 34.0 40.0 22.5 41.0 64-65 33.19034572125483 35.5 33.5 39.0 22.0 41.0 66-67 32.80268988413849 35.0 33.0 39.0 20.0 41.0 68-69 32.40949928856969 35.0 33.0 38.5 18.5 40.0 70-71 31.953184497594687 35.0 33.0 37.5 14.5 40.0 72-73 31.553345416356123 35.0 32.5 37.0 9.5 39.0 74-75 31.1441798224812 35.0 32.0 36.5 6.0 39.0 76-77 30.701853106579037 35.0 31.5 36.0 2.0 38.5 78-79 30.290201741310387 35.0 31.0 35.5 2.0 37.0 80-81 29.876842096347993 34.5 31.0 35.0 2.0 37.0 82-83 29.429181346974726 34.0 30.0 35.0 2.0 36.5 84-85 29.145916898163833 34.0 30.0 35.0 2.0 36.0 86-87 28.832093976556678 34.0 29.0 35.0 2.0 36.0 88-89 28.42662358560878 34.0 29.0 35.0 2.0 35.0 90-91 28.036156751812456 34.0 29.0 35.0 2.0 35.0 92-93 27.745878785825596 34.0 28.0 35.0 2.0 35.0 94-95 27.3611660681618 34.0 27.0 35.0 2.0 35.0 96-97 26.81810336066129 33.5 25.5 35.0 2.0 35.0 98-99 26.19159919371231 33.0 24.5 35.0 2.0 35.0 100-101 23.898989599566367 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38612124645020884 1101 2 0.5318023359268977 1101 3 0.380874903003523 1101 4 0.43621456807595393 1101 5 0.3794849906730562 1101 6 0.25445230252603324 1101 7 0.32816424743144523 1101 8 0.4851839367622759 1101 9 0.46296971776040863 1101 10-11 0.35037931574976966 1101 12-13 0.4183737172480235 1101 14-15 0.554826236991623 1101 16-17 0.31182648156980264 1101 18-19 0.3960504945305061 1101 20-21 0.31671024670242076 1101 22-23 0.3865538564417008 1101 24-25 0.40772831612719074 1101 26-27 0.4614081447320615 1101 28-29 0.3998910545836196 1101 30-31 0.49116723131556483 1101 32-33 0.48752454975358006 1101 34-35 0.4404536685081979 1101 36-37 0.42490928598202515 1101 38-39 0.38146351340276397 1101 40-41 0.3518982512455082 1101 42-43 0.263539644202325 1101 44-45 0.4433938336795151 1101 46-47 0.6016506559279193 1101 48-49 0.8146049895620351 1101 50-51 0.6747760276414425 1101 52-53 0.6302923401902305 1101 54-55 0.8935574578857839 1101 56-57 0.8494801741362394 1101 58-59 0.8219694627998493 1101 60-61 0.8105742705575594 1101 62-63 0.8042575436309036 1101 64-65 0.954436205601759 1101 66-67 0.9725408225826939 1101 68-69 1.1007459613250674 1101 70-71 1.2089033250249415 1101 72-73 1.0956305807648867 1101 74-75 0.8862625014663621 1101 76-77 0.9265874028144765 1101 78-79 0.818457075319035 1101 80-81 1.0736804749504891 1101 82-83 1.3493148774175943 1101 84-85 1.2286582430461301 1101 86-87 1.0011760213951817 1101 88-89 1.1338359303414194 1101 90-91 0.9275378933818992 1101 92-93 0.6697685654084076 1101 94-95 0.7777135634226404 1101 96-97 0.5441876806168437 1101 98-99 0.6268024788543549 1101 100-101 1.286632793416512 1102 1 0.16989621263147825 1102 2 0.1918206378287053 1102 3 0.11683018270432655 1102 4 0.189632249304573 1102 5 0.25983431827710035 1102 6 0.1391702145177547 1102 7 0.18887085998814257 1102 8 0.17248002970411136 1102 9 0.34247954508594347 1102 10-11 0.22885667709296342 1102 12-13 0.16819467690427103 1102 14-15 0.3470359927010236 1102 16-17 0.35394870644446996 1102 18-19 0.5211241000051601 1102 20-21 0.43649420468761235 1102 22-23 0.3953268332858215 1102 24-25 0.27829209427630985 1102 26-27 0.170256716387982 1102 28-29 0.20416614012511758 1102 30-31 0.3104996097670636 1102 32-33 -0.019563458522064536 1102 34-35 -0.08648394587869035 1102 36-37 0.05406302195719803 1102 38-39 0.20399872467037028 1102 40-41 0.2272821933160074 1102 42-43 0.06088586844040833 1102 44-45 -0.12274764797880522 1102 46-47 -0.09962848786137357 1102 48-49 -0.1841974294719435 1102 50-51 -0.006528181795971477 1102 52-53 -0.04588733674141565 1102 54-55 -0.1770520750178548 1102 56-57 -0.03366218283041178 1102 58-59 -0.028141940080715244 1102 60-61 -0.10404735751596661 1102 62-63 0.046093303955565545 1102 64-65 0.2944636584544682 1102 66-67 0.36515640307127484 1102 68-69 0.11550485366214502 1102 70-71 0.06875651194305732 1102 72-73 -0.04883110460155038 1102 74-75 -0.0487359070639215 1102 76-77 0.0652939357976905 1102 78-79 -0.3112536750589392 1102 80-81 -0.17094035738817226 1102 82-83 -0.1288959127471223 1102 84-85 -0.19336570857318947 1102 86-87 -0.21265385049150964 1102 88-89 -0.7544846712341382 1102 90-91 -0.8417586770008469 1102 92-93 -0.88918544111662 1102 94-95 -0.5822470477017347 1102 96-97 -0.8589196640159251 1102 98-99 -0.6923190298589432 1102 100-101 -0.6020120701149594 1103 1 0.14354602940857575 1103 2 0.30699256956886245 1103 3 0.256805449680364 1103 4 0.04041105004242951 1103 5 0.3500250686224504 1103 6 0.1741211207059763 1103 7 0.2200146175879638 1103 8 -0.013826229653496114 1103 9 0.2621716644331258 1103 10-11 0.19552810011039412 1103 12-13 0.2559121248532463 1103 14-15 0.2114014127901953 1103 16-17 0.46315038398871167 1103 18-19 0.2773880071611998 1103 20-21 0.26787433224053814 1103 22-23 0.18355548554281143 1103 24-25 0.23034704460346234 1103 26-27 0.19629049888626326 1103 28-29 0.029482954445448684 1103 30-31 -0.10703353992976616 1103 32-33 0.09624024067804271 1103 34-35 0.08947311461382412 1103 36-37 0.19972833202990614 1103 38-39 0.4082446983160537 1103 40-41 0.41766650645759995 1103 42-43 0.33935986567757936 1103 44-45 0.2494988385255752 1103 46-47 0.4565905441592122 1103 48-49 0.5883187214408636 1103 50-51 0.49263015091696616 1103 52-53 0.4367705947836811 1103 54-55 0.608951432273841 1103 56-57 0.4716256384300621 1103 58-59 0.2950810459149338 1103 60-61 0.16935388823433328 1103 62-63 0.2672387986573952 1103 64-65 0.43208246382459947 1103 66-67 0.4503881407523309 1103 68-69 0.373535848525286 1103 70-71 0.37102156889518767 1103 72-73 0.6966338183455711 1103 74-75 0.667792825430638 1103 76-77 0.48918922163368705 1103 78-79 0.38686797998568423 1103 80-81 0.3502750349900836 1103 82-83 0.0912786660056355 1103 84-85 0.1957813329766367 1103 86-87 0.4853840514201302 1103 88-89 0.6769277167848244 1103 90-91 0.693004043135474 1103 92-93 0.4937328489132433 1103 94-95 0.36482407421801 1103 96-97 0.4813765532376344 1103 98-99 0.5566511168021009 1103 100-101 0.989535274186931 1104 1 -0.0876333274372385 1104 2 -0.005823126572337856 1104 3 0.03703099777242613 1104 4 -0.08133689454592741 1104 5 0.10810168357356531 1104 6 0.004804926250962183 1104 7 0.061100325437941194 1104 8 0.0923815491419262 1104 9 0.1781744439404207 1104 10-11 0.18688251491573737 1104 12-13 -0.040379527912342894 1104 14-15 0.07411117740332429 1104 16-17 0.07363579901291928 1104 18-19 0.19038602878099908 1104 20-21 0.19149100673431008 1104 22-23 0.13723355144773564 1104 24-25 0.003912825213632232 1104 26-27 -0.22534035789473705 1104 28-29 -0.06856905294106497 1104 30-31 0.09953718981839899 1104 32-33 0.009741888619863914 1104 34-35 0.018821991654625947 1104 36-37 0.042295168767850555 1104 38-39 0.06829480594758763 1104 40-41 0.13216563201780218 1104 42-43 0.07227960209189632 1104 44-45 0.0566800823763387 1104 46-47 0.40200021335959235 1104 48-49 0.3282520387658181 1104 50-51 0.3354211757434058 1104 52-53 0.31393570984210584 1104 54-55 0.3806238329138907 1104 56-57 0.45792678997058545 1104 58-59 0.4486339489913078 1104 60-61 0.4291560077149015 1104 62-63 0.46907182846910445 1104 64-65 0.4878383601838223 1104 66-67 0.6422857069834365 1104 68-69 0.49257122349314386 1104 70-71 0.6100623838773274 1104 72-73 0.7230933253017717 1104 74-75 0.4685873650163046 1104 76-77 0.5937054959889103 1104 78-79 0.5952789645645211 1104 80-81 0.7391213057115458 1104 82-83 0.7963119542180159 1104 84-85 0.6233228927732313 1104 86-87 0.9349767941010789 1104 88-89 1.041273735693121 1104 90-91 0.9453888306455411 1104 92-93 0.9810688781703156 1104 94-95 1.1573257795349896 1104 96-97 0.9829394948403554 1104 98-99 1.2848586533468804 1104 100-101 1.614011725937619 1105 1 0.2481015365995134 1105 2 0.23817631554031493 1105 3 0.19504823126136017 1105 4 0.23236141915238306 1105 5 0.22681400983306332 1105 6 0.25199914514329436 1105 7 0.18456994890756562 1105 8 0.32200667499346736 1105 9 0.2861605141906409 1105 10-11 0.3663903972538094 1105 12-13 0.3443666115507753 1105 14-15 0.17338352667567136 1105 16-17 0.30821653359365797 1105 18-19 0.17254670905489178 1105 20-21 0.3230990298517682 1105 22-23 0.05119032389507794 1105 24-25 0.27920190238950227 1105 26-27 0.29477954430063846 1105 28-29 0.17675101313371755 1105 30-31 0.11792567990499947 1105 32-33 0.24667947933104273 1105 34-35 0.3524952875920704 1105 36-37 0.28106332734733286 1105 38-39 0.2550497757214245 1105 40-41 0.07559236789736445 1105 42-43 0.41513842608279106 1105 44-45 0.21333419657761965 1105 46-47 0.1510141038829218 1105 48-49 0.1451658320947118 1105 50-51 0.03664634866669303 1105 52-53 0.07672680282188082 1105 54-55 -0.03484206840106907 1105 56-57 -0.006864198405310162 1105 58-59 0.030174851286922433 1105 60-61 -0.015471493516372448 1105 62-63 0.0562602505631844 1105 64-65 0.129012190036903 1105 66-67 0.2726613656609871 1105 68-69 0.25447645237950667 1105 70-71 0.2728271079628115 1105 72-73 0.11044257585857409 1105 74-75 0.22629972178526714 1105 76-77 0.001651903935318444 1105 78-79 -0.11829164523250668 1105 80-81 0.0635992669819565 1105 82-83 0.1787273885202474 1105 84-85 0.3210830622315122 1105 86-87 0.4724761948080278 1105 88-89 0.5220015607605717 1105 90-91 0.627142427493478 1105 92-93 0.8623096980903817 1105 94-95 0.38620233388324365 1105 96-97 0.6135570499862126 1105 98-99 0.8465673141121464 1105 100-101 1.307605174248046 1106 1 0.18213431545987646 1106 2 0.09692220876896585 1106 3 0.14737673531400475 1106 4 0.13387892411816438 1106 5 0.06184182761949586 1106 6 0.09095722548068608 1106 7 0.1442929948375138 1106 8 0.2670065337864287 1106 9 0.47229021756172784 1106 10-11 0.26986361969254347 1106 12-13 0.3485251588127696 1106 14-15 0.4526981077143901 1106 16-17 0.3927218853403929 1106 18-19 0.3253233101073576 1106 20-21 0.2979853581863736 1106 22-23 0.34439967744925326 1106 24-25 0.1928843936582041 1106 26-27 0.0642802362151258 1106 28-29 0.17318788888093906 1106 30-31 0.27509146638113435 1106 32-33 -0.15065594386644676 1106 34-35 0.12370458466343592 1106 36-37 0.09398518337263795 1106 38-39 0.29098932028165336 1106 40-41 0.2191175545260009 1106 42-43 -0.35595790535836613 1106 44-45 -0.03907338341617361 1106 46-47 -0.16355752103353183 1106 48-49 0.07953280094443471 1106 50-51 -0.022844176341074274 1106 52-53 0.1515029896670299 1106 54-55 0.18400295216808615 1106 56-57 0.11118488516101621 1106 58-59 0.19322426433634377 1106 60-61 0.16264941736315564 1106 62-63 -0.030523241392721445 1106 64-65 -0.30164574062103355 1106 66-67 -0.08860978009291642 1106 68-69 0.047728168045019004 1106 70-71 0.07244255412997802 1106 72-73 0.03130544499730448 1106 74-75 -0.23323504809432904 1106 76-77 -0.15985129205063942 1106 78-79 0.12085894736636149 1106 80-81 0.33246452722653075 1106 82-83 0.6053703324045685 1106 84-85 0.33830326048619597 1106 86-87 0.3811199482793661 1106 88-89 0.29995584733554637 1106 90-91 0.2984924609124775 1106 92-93 0.34199471225815614 1106 94-95 0.5454626914194627 1106 96-97 0.7919933232500718 1106 98-99 0.951615936402149 1106 100-101 1.4523376810495172 1107 1 0.1380527435491885 1107 2 0.23448119622497643 1107 3 0.1145741890721581 1107 4 0.13447179905755746 1107 5 0.1362824369125164 1107 6 0.26032983417842814 1107 7 0.1763082415646977 1107 8 0.2711878623063555 1107 9 0.2321110589160753 1107 10-11 0.21587119505007024 1107 12-13 0.09928845529345409 1107 14-15 0.027580372832709088 1107 16-17 -0.020957203696468696 1107 18-19 0.3596572499173405 1107 20-21 0.4258231168790658 1107 22-23 0.328393254237362 1107 24-25 0.2694984776784253 1107 26-27 0.1756447126849494 1107 28-29 0.10190127825335793 1107 30-31 0.11940418750908321 1107 32-33 0.09928284839688928 1107 34-35 0.15515936054710977 1107 36-37 0.1198772561306356 1107 38-39 0.10828662666118305 1107 40-41 0.09538551479618462 1107 42-43 0.2583361414091456 1107 44-45 0.10135276644917468 1107 46-47 0.3487248314558187 1107 48-49 0.30643468134211815 1107 50-51 0.2094523859443953 1107 52-53 0.18893932161938665 1107 54-55 0.09683593425702952 1107 56-57 0.22766320802102058 1107 58-59 0.10673533213530817 1107 60-61 -0.08258838069451713 1107 62-63 -0.23554035555594766 1107 64-65 -0.007548566738250884 1107 66-67 0.05668658536767879 1107 68-69 0.014306343526392595 1107 70-71 -0.26143724827704773 1107 72-73 -0.3694504105428358 1107 74-75 -0.2919884745274324 1107 76-77 -0.12805207262930196 1107 78-79 -0.2746570458551787 1107 80-81 -0.13299510687122407 1107 82-83 0.2071443966462887 1107 84-85 0.043590408781742696 1107 86-87 -0.04194672734842797 1107 88-89 0.025056300032765932 1107 90-91 0.1991823081091617 1107 92-93 0.08887431784030397 1107 94-95 -0.10940008323525419 1107 96-97 -0.029931768081471688 1107 98-99 0.14826679307043378 1107 100-101 0.5464703794225905 1108 1 0.10567898375350637 1108 2 0.22245701317606148 1108 3 0.180067452436937 1108 4 0.2279840776414943 1108 5 0.4312627582557482 1108 6 0.1620313271624525 1108 7 0.032160210178609816 1108 8 0.21819765424633886 1108 9 0.39499247318546793 1108 10-11 0.09984942631544413 1108 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2316 94-95 -3.640674542993633 2316 96-97 -3.9002567638276027 2316 98-99 -4.497040579495746 2316 100-101 -5.033879167236297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1118.0 3 329.0 4 308.0 5 283.0 6 316.0 7 364.0 8 448.0 9 514.0 10 700.0 11 1028.0 12 1597.0 13 2467.0 14 2654.0 15 3896.0 16 5530.0 17 8532.0 18 7096.0 19 3880.0 20 3605.0 21 3658.0 22 3915.0 23 4279.0 24 4697.0 25 4888.0 26 5790.0 27 6601.0 28 7897.0 29 9611.0 30 11941.0 31 15379.0 32 20935.0 33 29417.0 34 44563.0 35 75522.0 36 94022.0 37 113540.0 38 78920.0 39 10120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.694364008817516 18.370555872779736 14.202230133059071 20.73284998534368 2 15.762410055792095 21.224884663019484 39.231007730923864 23.78169755026456 3 19.438204150783566 28.170266836086405 29.39398559932232 22.99754341380771 4 12.165398166648872 17.066197567394887 36.495177666265015 34.27322659969122 5 12.808315058417591 38.85998420504964 32.301351383413945 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25.91225419111768 32.56747462290105 20.620671663030073 28-29 20.040723295876596 26.287023006798794 33.20972920871621 20.462524488608402 30-31 19.96320599139271 26.946783361722204 32.620887037142126 20.469123609742958 32-33 19.591403568802956 27.301562833495385 32.04579410439443 21.061239493307223 34-35 20.52319081176311 26.915232416316577 31.659015449247867 20.90256132267245 36-37 20.892337897849366 26.723551598342915 31.771454030012812 20.612656473794903 38-39 20.875430286767497 26.545983086680764 31.955653358269636 20.622933268282107 40-41 20.03972219869569 27.12907597188109 32.567036503768946 20.264165325654275 42-43 20.106496777739853 27.739641041320652 31.309070181664495 20.844791999275003 44-45 19.75143537853424 28.114532154257777 30.867308264002936 21.26672420320505 46-47 20.14614848435315 28.09902796351708 30.133215664718982 21.621607887410786 48-49 20.667581935938493 28.121069628502877 29.520059557435406 21.69128887812323 50-51 20.766499056930567 28.542438971508037 29.430457204466094 21.260604767095302 52-53 20.58894275560434 28.483465628631343 29.58510771649168 21.34248389927263 54-55 19.79955160406995 28.335251763219166 29.904383421716624 21.960813210994253 56-57 20.602886952131755 29.02317812076205 28.62726196960238 21.74667295750381 58-59 20.666738945563825 28.12600583426223 29.11396800981186 22.093287210362085 60-61 20.48830896023649 27.93499942825924 29.788540621122223 21.78815099038205 62-63 21.230457021327776 28.010607563351904 29.56913337969131 21.189802035629015 64-65 20.88344400027102 28.21159970187682 29.80571176909005 21.099244528762114 66-67 20.530188364416833 28.488519333207414 29.547015721174247 21.4342765812015 68-69 20.41981677488215 28.66511649576668 29.662816652054403 21.25225007729677 70-71 20.61084543284664 28.573146788229298 29.0473867253474 21.768621053576663 72-73 20.521932974672392 28.44324664941119 28.76399184204464 22.270828533871775 74-75 20.294391917816224 28.871294374131317 29.000034726015965 21.834278982036487 76-77 20.59463268701064 28.120966129890196 29.61356159603194 21.670839587067224 78-79 20.341577160203652 28.125992575219733 29.91908632683432 21.613343937742293 80-81 20.15900874497274 29.14471388251717 29.266520070068204 21.429757302441885 82-83 20.247236691652905 28.602041248464825 28.925083640367593 22.225638419514674 84-85 20.22689866474888 28.1904607010259 28.775707798296864 22.80693283592836 86-87 20.71168849208534 28.03345933662359 29.065850623847574 22.1890015474435 88-89 20.912227088660558 27.605494943882558 29.055290776513598 22.426987190943287 90-91 20.339772604076323 27.884035532307028 29.407574669340853 22.368617194275803 92-93 20.342041218092994 28.358468141916017 29.13864537175318 22.160845268237807 94-95 20.129698522087374 28.16783896783287 28.92051730330074 22.781945206779017 96-97 20.32252791139503 27.72166276614397 28.58562219054371 23.370187131917287 98-99 20.423909074968087 27.793482192549018 28.37984936750787 23.402759364975022 100-101 20.89638104243398 27.48892825884532 28.647658942077868 22.967031756642832 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2406.0 1 1752.5 2 2070.0 3 2726.0 4 1910.0 5 1638.0 6 2676.5 7 4882.0 8 7093.0 9 7424.0 10 5283.5 11 3146.5 12 2703.5 13 2701.0 14 2539.0 15 2317.0 16 2048.5 17 1848.5 18 1749.0 19 1664.0 20 1680.0 21 1740.0 22 1817.0 23 1943.0 24 2141.5 25 2375.0 26 2683.5 27 3190.5 28 3661.0 29 4362.0 30 5319.0 31 6317.5 32 7798.0 33 9038.5 34 10067.0 35 10980.5 36 11771.5 37 12477.5 38 12869.0 39 13078.0 40 13018.0 41 13687.5 42 14246.5 43 15015.5 44 16186.0 45 17022.0 46 17349.0 47 19404.5 48 31219.5 49 31161.0 50 20815.5 51 18256.5 52 15935.5 53 15886.5 54 16309.0 55 15882.0 56 15022.5 57 13843.0 58 13075.5 59 12370.5 60 10979.0 61 9527.0 62 8288.0 63 6582.0 64 5030.5 65 4272.0 66 3414.0 67 2672.0 68 1998.0 69 1348.0 70 1080.5 71 930.5 72 794.0 73 772.0 74 672.0 75 496.5 76 345.0 77 226.0 78 154.5 79 112.0 80 87.5 81 62.5 82 40.5 83 25.0 84 20.5 85 13.0 86 8.5 87 6.0 88 3.5 89 1.5 90 1.5 91 1.5 92 1.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028965377058066268 2 0.022528626600718204 3 0.018632698692323327 4 0.049630733789552135 5 0.050477674639203204 6 0.04573480588115726 7 0.04471847686157599 8 0.0392980554238092 9 0.037265397384646654 10-11 0.04421031235178535 12-13 0.03938274950877431 14-15 0.035740903855274744 16-17 0.040737854868216 18-19 0.04378684192695982 20-21 0.010840842875533573 22-23 0.01812453418253269 24-25 0.0047428687580459376 26-27 0.010840842875533573 28-29 0.0058438918625923165 30-31 0.007876549901754862 32-33 0.003726539738464666 34-35 0.006775526797208484 36-37 0.00770716173182465 38-39 0.009485737516091875 40-41 0.001693881699302121 42-43 0.002286740294057863 44-45 0.0017785757842672266 46-47 2.540822548953181E-4 48-49 9.316349346161664E-4 50-51 8.469408496510604E-5 52-53 0.0023714343790229688 54-55 0.005674503692662104 56-57 0.014143912189172709 58-59 0.009146961176231452 60-61 0.009739819770987194 62-63 0.00448878650315062 64-65 0.0 66-67 0.0070296090521038005 68-69 0.017362287417846737 70-71 0.007961243986719968 72-73 0.002710210718883393 74-75 0.004150010163290196 76-77 0.007791855816789756 78-79 0.006690832712243377 80-81 0.013212277254556542 82-83 0.005928585947557422 84-85 0.016345958398265466 86-87 0.00533572735280168 88-89 0.006013280032522529 90-91 0.00406531607832509 92-93 0.018548004607358223 94-95 0.011518395555254421 96-97 0.009231655261196558 98-99 0.019564333626939493 100-101 0.039213361338844094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 590360.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.00317503660876 #Duplication Level Percentage of deduplicated Percentage of total 1 71.19202801530142 29.190991859225583 2 14.03526693831069 11.509810139141623 3 5.566789145474745 6.847680891713841 4 2.7176312114656476 4.457260329947083 5 1.5410567687032974 3.1594110214246003 6 1.0128411495668452 2.4917821763981634 7 0.7071175005638015 2.0295843852946795 8 0.49089389586963555 1.6102566669396368 9 0.3577168262840093 1.320077307749706 >10 2.1054810578355756 16.32855224583383 >50 0.17977641218227572 4.98340781290678 >100 0.0801761469812583 5.83442365668877 >500 0.00619918662226224 1.7015233208484442 >1k 0.005785907514111423 4.628224772334699 >5k 8.265582163016319E-4 2.1856635586398996 >10k+ 4.1327910815081595E-4 1.7213498549127078 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10158 1.7206450301510943 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7718 1.3073378955213768 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5180 0.8774307202384986 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4244 0.7188833931838201 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3060 0.518327799986449 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2651 0.4490480384849922 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2438 0.412968358289857 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2271 0.38468053391151164 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.33708245816112203 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1653 0.27999864489464055 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1550 0.2625516633918287 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1368 0.23172301646453014 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1342 0.22731892404634463 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1322 0.22393116064774038 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1275 0.21596991666102042 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1141 0.19327190189037197 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1007 0.17057388711972354 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 923 0.15634528084558574 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 856 0.14499627346026153 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 835 0.14143912189172708 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 823 0.13940646385256453 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 793 0.13432481875465818 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 767 0.12992072633647267 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 651 0.11027169862456805 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 603 0.10214106646791789 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.540822548953181E-4 0.0 24-25 0.0 0.0 0.0 0.0012704112744765907 0.0 26-27 0.0 0.0 0.0 0.002117352124127651 0.0 28-29 0.0 0.0 0.0 0.004404092418185514 0.0 30-31 0.0 0.0 0.0 0.008892878921336134 0.0 32-33 0.0 0.0 0.0 0.014228606274137814 0.0 34-35 0.0 0.0 0.0 0.021681685751067145 0.0 36-37 0.0 0.0 0.0 0.03269191679653093 0.0 38-39 0.0 0.0 0.0 0.05073175689409852 0.0 40-41 0.0 0.0 0.0 0.07334507757978183 0.0 42-43 0.0 0.0 0.0 0.09612778643539535 0.0 44-45 0.0 0.0 0.0 0.12162070600989228 0.0 46-47 0.0 0.0 0.0 0.15829324479978318 0.0 48-49 0.0 0.0 0.0 0.19513517175960432 0.0 50-51 0.0 0.0 0.0 0.23553425028795988 0.0 52-53 0.0 0.0 0.0 0.2783047631953385 0.0 54-55 0.0 0.0 0.0 0.32048241750796125 0.0 56-57 0.0 0.0 0.0 0.3751101023104546 0.0 58-59 0.0 0.0 0.0 0.43990107730876077 0.0 60-61 0.0 0.0 0.0 0.5147706484179145 0.0 62-63 0.0 0.0 0.0 0.5916728775662308 0.0 64-65 0.0 0.0 0.0 0.6650179551460126 0.0 66-67 0.0 0.0 0.0 0.7599600243918965 0.0 68-69 0.0 0.0 0.0 0.8659123246832441 0.0 70-71 0.0 0.0 0.0 0.9854156785690087 0.0 72-73 0.0 0.0 0.0 1.1159292635002371 0.0 74-75 0.0 0.0 0.0 1.250846940849651 0.0 76-77 0.0 0.0 0.0 1.3963513788197033 0.0 78-79 0.0 0.0 0.0 1.5529507419201845 0.0 80-81 0.0 0.0 0.0 1.7310624026018022 0.0 82-83 0.0 0.0 0.0 1.928823090995325 0.0 84-85 0.0 0.0 0.0 2.157327732231181 0.0 86-87 0.0 0.0 0.0 2.3872721729114437 0.0 88-89 0.0 0.0 0.0 2.6178094721864626 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAAG 105 4.6362402E-8 36.203583 1 GTATAGT 155 1.3478711E-9 30.656263 1 CTTGACA 335 0.0 28.36848 9 TAGATAG 85 6.38991E-4 27.951296 5 ACAGCGT 90 8.9234766E-4 26.398449 8 AAGACTT 380 0.0 26.259508 5 CAAGACT 420 0.0 26.023531 4 GTGTAGG 240 9.094947E-12 25.738485 1 GAGTACT 5250 0.0 25.611864 12-13 ATGGGTA 205 9.513315E-10 25.497036 5 ACTTGAC 335 0.0 24.113209 8 TACATGG 6375 0.0 23.92631 2 GTACATG 6340 0.0 23.908499 1 TAAGGGT 160 1.8497958E-6 23.760618 4 CGGTTAC 180 0.0 23.748539 28-29 GTACTTT 5685 0.0 23.482975 14-15 ATGGGAG 1075 0.0 22.985067 5 GCTTATG 105 0.0021903494 22.62724 1 TATAGTC 105 0.0021903494 22.62724 3 GACTTGA 380 0.0 22.510057 7 >>END_MODULE