FastQCFastQC Report
Thu 26 May 2016
SRR936389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590360
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87541.4828240395690764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81771.3850870655193441No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58660.9936310048106239No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54940.930618605596585No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34380.5823565282200691No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23670.400941798224812No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.3836642048919303No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22450.3802764414933261No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21130.3579172030625381No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.3103191273121485No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.2722067890778508No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.23832915509180838No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13980.23680466156243649No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT10080.17074327528965377No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC9850.1668473473812589No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9150.15499017548614405No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA9030.1529575174469815No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG8770.14855342502879598No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT8590.14550443797005216No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA8580.14533504980012196No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7880.13347787790500712No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG7540.1277186801273799No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG6850.11603089640219526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT404.491602E-429.68988418-19
TAAGGTA1153.860516E-628.9129164
CGATTCG458.9390797E-426.39324238-39
ACTTGAC2251.0186341E-1025.3310748
CTTGACA2301.3460522E-1024.78049
GTAAGGT2202.1627784E-923.7498933
TAGTTCC1652.42665E-623.0302014
GAGTACT65900.022.52265212-13
GTAGTCT2354.642061E-922.2320619
ATGCACT3059.094947E-1221.803184
AGTACTT67200.021.27414512-13
GTACTTT70600.021.05690414-15
TTAGGGT3252.1827873E-1120.4614474
GGCCATT3252.1827873E-1120.4597137
GTCGGGA2351.1285738E-720.2143882
TACGATT600.004756719.79660836-37
ACTTTTT76500.019.7450116-17
CATGCAC2902.4319888E-919.6550853
TACTTTT73700.019.33341814-15
GACTTGA3759.094947E-1218.9983067
Produced by FastQC (version 0.11.5)