##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936389_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 590360 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.98885934006369 31.0 31.0 33.0 30.0 34.0 2 31.08126228064232 31.0 31.0 34.0 28.0 34.0 3 31.084936310048107 31.0 31.0 34.0 28.0 34.0 4 34.98917101429636 35.0 35.0 37.0 32.0 37.0 5 34.71175892675655 35.0 35.0 37.0 32.0 37.0 6 34.92151060369944 35.0 35.0 37.0 32.0 37.0 7 34.8363981299546 35.0 35.0 37.0 32.0 37.0 8 34.841323937936174 35.0 35.0 37.0 32.0 37.0 9 36.240668405718544 38.0 35.0 39.0 32.0 39.0 10-11 36.04946219256047 37.0 35.0 39.0 30.5 39.0 12-13 35.88903804458297 37.0 35.0 39.0 30.0 39.0 14-15 36.55520445152111 38.0 35.0 40.0 30.0 41.0 16-17 36.44798512771868 38.0 35.0 40.0 30.5 41.0 18-19 36.71759773697405 38.5 35.0 40.0 31.0 41.0 20-21 36.79397316891388 39.0 35.0 40.0 31.0 41.0 22-23 36.64313130970933 38.0 35.0 40.0 30.5 41.0 24-25 36.39972135646046 38.0 35.0 40.0 30.0 41.0 26-27 35.941944745578965 38.0 34.5 40.0 29.5 41.0 28-29 35.647921607154956 38.0 34.0 40.0 28.0 41.0 30-31 35.36922894505048 37.5 34.0 40.0 27.0 41.0 32-33 34.99661223660139 37.0 33.5 40.0 27.0 41.0 34-35 34.64045582356528 37.0 33.0 40.0 25.5 41.0 36-37 34.17947947015381 37.0 33.0 40.0 24.5 41.0 38-39 33.791943051697274 36.0 33.0 39.0 24.0 41.0 40-41 33.338263940646385 36.0 32.5 39.0 22.0 40.0 42-43 32.866264652076694 35.5 31.0 39.0 20.5 40.0 44-45 32.36641879531133 35.0 31.0 39.0 18.0 40.0 46-47 31.656563791584794 35.0 30.0 39.0 12.0 40.0 48-49 30.990709905820175 35.0 29.5 39.0 7.5 40.0 50-51 30.47531760281862 35.0 28.0 38.0 4.0 40.0 52-53 30.030759197777627 35.0 27.5 38.0 2.0 40.0 54-55 29.613540890304222 34.0 27.0 38.0 2.0 40.0 56-57 29.185033708245818 34.0 26.0 38.0 2.0 40.0 58-59 28.70875990920794 33.5 26.0 37.5 2.0 40.0 60-61 28.18496849380039 33.0 25.5 37.0 2.0 39.0 62-63 27.597997831831425 33.0 23.5 36.0 2.0 39.0 64-65 27.006873771935766 32.0 22.0 36.0 2.0 39.0 66-67 26.373294261128805 31.5 21.5 35.0 2.0 38.0 68-69 26.016755877769498 31.0 20.5 35.0 2.0 38.0 70-71 25.465626058676065 31.0 19.5 35.0 2.0 37.5 72-73 24.868393014431874 31.0 18.0 34.5 2.0 36.5 74-75 24.20279321092215 30.0 14.0 34.0 2.0 36.0 76-77 23.2368927434108 28.5 13.0 33.0 2.0 35.0 78-79 23.891863439257403 30.0 11.5 34.0 2.0 35.0 80-81 23.598480588115727 30.0 7.0 34.0 2.0 35.0 82-83 23.108334744901416 30.0 4.5 34.0 2.0 35.0 84-85 22.465526966596652 29.0 2.0 34.0 2.0 35.0 86-87 21.824166610203946 29.0 2.0 34.0 2.0 35.0 88-89 21.10893014431872 28.5 2.0 33.0 2.0 35.0 90-91 20.264633444000268 27.0 2.0 33.0 2.0 35.0 92-93 19.42351870045396 25.5 2.0 33.0 2.0 34.5 94-95 18.45519513517176 24.5 2.0 32.0 2.0 34.0 96-97 17.24361999457958 21.5 2.0 32.0 2.0 34.0 98-99 15.872052645843214 7.5 2.0 31.5 2.0 34.0 100-101 13.162124805203604 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26775692255771943 1101 2 0.40025154384334627 1101 3 0.29375806800861426 1101 4 0.1854971062427353 1101 5 0.09171204750295203 1101 6 0.2988994292634004 1101 7 0.17705088719446138 1101 8 0.3824820139372136 1101 9 0.5194728443476606 1101 10-11 0.4506419220406883 1101 12-13 0.4515501966830229 1101 14-15 0.4315894449414941 1101 16-17 0.372408486332219 1101 18-19 0.47249895372959116 1101 20-21 0.42573207190687157 1101 22-23 0.30333429772279175 1101 24-25 0.28258952457197495 1101 26-27 0.6189748652465852 1101 28-29 0.4395243517219356 1101 30-31 0.6446867364906836 1101 32-33 0.6566803987709662 1101 34-35 0.6858303359353499 1101 36-37 0.7733024007618994 1101 38-39 0.6790259357262656 1101 40-41 0.860451377975842 1101 42-43 0.8755660961875478 1101 44-45 1.164367475631913 1101 46-47 1.324934807855275 1101 48-49 1.6563743433481193 1101 50-51 1.1072163679801719 1101 52-53 1.350230164093439 1101 54-55 1.0186127781315903 1101 56-57 1.0337927679732317 1101 58-59 1.2111866640890305 1101 60-61 1.2691008620051925 1101 62-63 1.1888322767829784 1101 64-65 1.0215418452615879 1101 66-67 1.0466977784338987 1101 68-69 1.3558193998554344 1101 70-71 0.8104307646843623 1101 72-73 0.7526035999917404 1101 74-75 0.8680577108069514 1101 76-77 0.7226540591430926 1101 78-79 0.866102894110309 1101 80-81 1.2940026849364408 1101 82-83 1.362606177967674 1101 84-85 1.704258880070416 1101 86-87 1.4027937479797181 1101 88-89 1.0842348372041037 1101 90-91 0.8578949152750184 1101 92-93 0.9167888547931398 1101 94-95 0.9972717249765175 1101 96-97 1.0488656940879686 1101 98-99 1.2005686757698957 1101 100-101 1.3296727250603944 1102 1 0.3687038470424788 1102 2 0.5681436421961763 1102 3 0.358252378504357 1102 4 0.30317435618304955 1102 5 0.2774445214426322 1102 6 0.23165585482239948 1102 7 0.4467691970536194 1102 8 0.2841530571456161 1102 9 0.45366955000533693 1102 10-11 0.5232586961315633 1102 12-13 0.6360055161705773 1102 14-15 0.5047831268067 1102 16-17 0.41826867571711546 1102 18-19 0.4798992879382311 1102 20-21 0.40498726407683705 1102 22-23 0.12711682338524355 1102 24-25 0.557633289989333 1102 26-27 0.47351254329225867 1102 28-29 0.011669652907578154 1102 30-31 0.4927312180694159 1102 32-33 0.40834945264222 1102 34-35 0.3419435690060908 1102 36-37 0.4291212119340173 1102 38-39 0.4450580038411758 1102 40-41 0.7295072385630945 1102 42-43 0.7172128694826867 1102 44-45 0.610659351178974 1102 46-47 0.8028352614241392 1102 48-49 0.5471159682387068 1102 50-51 0.48647156015014303 1102 52-53 0.5488873615152592 1102 54-55 0.3570646759592364 1102 56-57 0.20583462362692728 1102 58-59 0.30394348751546474 1102 60-61 0.5237340282342053 1102 62-63 0.5405033199913838 1102 64-65 0.5525277607545434 1102 66-67 0.41072061602441323 1102 68-69 0.04945551030343509 1102 70-71 0.2348598249214895 1102 72-73 0.06490765028218348 1102 74-75 0.1491888479120682 1102 76-77 0.09655992370901956 1102 78-79 0.0021735553659816276 1102 80-81 0.6833119870769657 1102 82-83 0.6723758127253738 1102 84-85 0.6035486867068407 1102 86-87 0.8107491072540078 1102 88-89 0.8148328319522555 1102 90-91 0.7047796132936384 1102 92-93 1.0064641949697872 1102 94-95 1.4368456089583752 1102 96-97 1.6215083295618307 1102 98-99 1.943528411585925 1102 100-101 2.2341049682369682 1103 1 0.14089118935911316 1103 2 0.2241354033694627 1103 3 0.07258181576602496 1103 4 0.08192339457300335 1103 5 -0.10707128204977323 1103 6 0.012348793089103083 1103 7 0.051820441563137365 1103 8 0.02695028770868646 1103 9 0.07445397564496403 1103 10-11 -0.23629639895409582 1103 12-13 0.04089711273276464 1103 14-15 -0.14295636579407045 1103 16-17 0.27626161977416075 1103 18-19 -0.1969997368030576 1103 20-21 0.13615831899407738 1103 22-23 0.0075266553826551785 1103 24-25 -0.22162964108080274 1103 26-27 -0.0030293415461457585 1103 28-29 0.16802331033451878 1103 30-31 -0.07147848323175054 1103 32-33 0.08413693889016116 1103 34-35 0.24971068980084965 1103 36-37 0.24265261625691892 1103 38-39 0.44035025057583965 1103 40-41 0.5104307564428368 1103 42-43 0.46006076552126274 1103 44-45 0.6909527559816482 1103 46-47 0.5972957671689905 1103 48-49 0.5494321042620669 1103 50-51 0.8841872709971028 1103 52-53 0.9772660249393397 1103 54-55 0.5130099076141974 1103 56-57 0.43148831165995105 1103 58-59 0.7969402780126913 1103 60-61 0.6878912028791326 1103 62-63 0.7752478419160944 1103 64-65 0.8285160477237952 1103 66-67 1.3978402113623645 1103 68-69 1.226279053826108 1103 70-71 1.0925580820291323 1103 72-73 0.8792538381704489 1103 74-75 0.6302120423599575 1103 76-77 0.43245238055030555 1103 78-79 0.3705880775388479 1103 80-81 0.774995817965948 1103 82-83 0.6203340974612068 1103 84-85 0.421187096514025 1103 86-87 0.16046186502754267 1103 88-89 0.40569257337221387 1103 90-91 0.37879380789869543 1103 92-93 0.5882630881876452 1103 94-95 0.6857009828075462 1103 96-97 0.6108251521540815 1103 98-99 0.7264971602934356 1103 100-101 0.8491652291331473 1104 1 -0.026960876252559274 1104 2 0.11013522911900608 1104 3 -0.07786645416068438 1104 4 0.05242406713727377 1104 5 -0.08428909724653266 1104 6 -0.14215823749184864 1104 7 -0.022389003236654048 1104 8 -0.06741270999418703 1104 9 0.07508722730725026 1104 10-11 -0.16534777521395938 1104 12-13 -0.2520189686719476 1104 14-15 -0.14588249847517432 1104 16-17 -0.06892003632768251 1104 18-19 -0.2814718960424827 1104 20-21 -0.235685782096688 1104 22-23 -0.27104988838076594 1104 24-25 -0.4664302836824916 1104 26-27 -0.36994661720399336 1104 28-29 -0.44969456117489415 1104 30-31 -0.36095524310824345 1104 32-33 -0.21065233493168734 1104 34-35 -0.2836242727034204 1104 36-37 -0.07342177593836396 1104 38-39 -0.0946570913184317 1104 40-41 0.3612659898853252 1104 42-43 0.6209247502892197 1104 44-45 0.43974309655629185 1104 46-47 0.41849354781315995 1104 48-49 0.4597462438366833 1104 50-51 0.5254066416979519 1104 52-53 0.39786094150099416 1104 54-55 0.431241817972964 1104 56-57 0.6618176662745086 1104 58-59 0.2889141319440576 1104 60-61 0.3092938340610374 1104 62-63 0.8072249258559374 1104 64-65 0.9291605619156442 1104 66-67 0.31541443249469125 1104 68-69 0.4338177236151175 1104 70-71 0.2983924973444765 1104 72-73 0.15778256930836676 1104 74-75 0.20394867341861556 1104 76-77 0.34183576768169743 1104 78-79 0.3451294482099456 1104 80-81 0.45673904504192464 1104 82-83 0.4759261558903596 1104 84-85 0.4111825702521408 1104 86-87 0.5856531686547122 1104 88-89 0.6521092827160757 1104 90-91 0.7954723391798417 1104 92-93 0.45691436839725696 1104 94-95 0.5176523541798943 1104 96-97 0.47705923851856014 1104 98-99 0.8016756076368612 1104 100-101 0.8126905708871686 1105 1 0.049062171468602145 1105 2 0.0039862644794759206 1105 3 0.09340278314628847 1105 4 0.044267143886010274 1105 5 -0.029956099517313817 1105 6 0.13765931591061076 1105 7 -0.1650926648082418 1105 8 0.25399578122562616 1105 9 0.19672192441364444 1105 10-11 0.13094053050831178 1105 12-13 0.21367640740078997 1105 14-15 0.2034584970922424 1105 16-17 0.4336972398744976 1105 18-19 0.26494280603260023 1105 20-21 0.24912377346758063 1105 22-23 0.23462051351604885 1105 24-25 0.22490437463329727 1105 26-27 0.30107527551601265 1105 28-29 0.4864240683399643 1105 30-31 0.15691022758741013 1105 32-33 0.37364313615623956 1105 34-35 0.6131872703345351 1105 36-37 -0.007427625038552321 1105 38-39 0.7071272765036625 1105 40-41 0.8783403937521896 1105 42-43 0.5692449863453106 1105 44-45 0.4548213097477145 1105 46-47 0.8546307009314482 1105 48-49 0.5665718648132447 1105 50-51 0.811839300772121 1105 52-53 1.0045612557484773 1105 54-55 0.9356199261246552 1105 56-57 0.943288178877097 1105 58-59 0.4037996996598139 1105 60-61 0.5486296866044391 1105 62-63 0.658994692720043 1105 64-65 0.6125878208146034 1105 66-67 1.0362168749952474 1105 68-69 0.8972248898524704 1105 70-71 0.6796605578578188 1105 72-73 0.9304532806583232 1105 74-75 0.7710585990191063 1105 76-77 0.7563743992014622 1105 78-79 0.874265986780447 1105 80-81 1.1714526701329042 1105 82-83 0.8475488671357176 1105 84-85 0.6251868502941598 1105 86-87 0.5676776459622097 1105 88-89 0.707799951614291 1105 90-91 0.9000511560228084 1105 92-93 0.775322035861528 1105 94-95 0.22595463323689202 1105 96-97 0.7680574210261746 1105 98-99 0.9323116863861483 1105 100-101 0.6914394213341328 1106 1 0.03724784930945191 1106 2 0.05892521597015232 1106 3 -0.015284273816629224 1106 4 -0.20735909049884782 1106 5 0.126115332416191 1106 6 0.031024467896450858 1106 7 0.12937107793135993 1106 8 -0.030070416633677155 1106 9 -0.04352772962911189 1106 10-11 0.1306675302353142 1106 12-13 0.030542646680821406 1106 14-15 0.07118280447171799 1106 16-17 0.21156915567744505 1106 18-19 0.19290603571996456 1106 20-21 0.028798432452589395 1106 22-23 0.17256472732233163 1106 24-25 -0.021774906528733595 1106 26-27 0.2286242978926225 1106 28-29 0.3797115564550353 1106 30-31 0.5098242701566278 1106 32-33 0.09953438790956426 1106 34-35 0.16234144017835916 1106 36-37 -0.021352992412197125 1106 38-39 0.28267068825397956 1106 40-41 0.03050745109165831 1106 42-43 -0.007830521764681464 1106 44-45 0.1942849112802847 1106 46-47 0.3566381943182506 1106 48-49 0.15515574650057573 1106 50-51 0.34069025203686465 1106 52-53 -0.14683151633395042 1106 54-55 0.11338075905790035 1106 56-57 0.17643041201932874 1106 58-59 0.0672774407237604 1106 60-61 0.24259638057113264 1106 62-63 0.43709489323403616 1106 64-65 0.0776061306604987 1106 66-67 0.07367910211264928 1106 68-69 0.23418489060557945 1106 70-71 0.30705754732377244 1106 72-73 0.004963527582365401 1106 74-75 0.21754113170853628 1106 76-77 -0.013062749546030261 1106 78-79 0.27075652120201354 1106 80-81 0.3382275955285188 1106 82-83 0.9016476367517292 1106 84-85 0.9285513433138242 1106 86-87 0.3713928031256408 1106 88-89 0.08185504118662124 1106 90-91 0.36243595461450084 1106 92-93 0.6466400623519704 1106 94-95 0.8403111527658247 1106 96-97 0.6962254009182942 1106 98-99 1.1700949241693852 1106 100-101 1.2594047220580542 1107 1 0.011106144998027645 1107 2 0.023110288963376746 1107 3 0.01637092740080348 1107 4 -0.14529684583111901 1107 5 0.059108462747865076 1107 6 -0.0071355555305103735 1107 7 -0.1906733756875738 1107 8 -0.008148445696548379 1107 9 0.03895389604837618 1107 10-11 -0.10293959770566374 1107 12-13 -0.2820800688304175 1107 14-15 -0.15508419567709808 1107 16-17 -0.19364526342513244 1107 18-19 -0.13113972366402038 1107 20-21 0.029730950322850447 1107 22-23 -0.11468438096495248 1107 24-25 0.1066065208836875 1107 26-27 0.33392320767481465 1107 28-29 -0.08230124196268207 1107 30-31 -0.09789894605790295 1107 32-33 -0.03217386001297484 1107 34-35 -0.07595708075469076 1107 36-37 0.08090113250919728 1107 38-39 -0.152911169292814 1107 40-41 0.01559556622940761 1107 42-43 -0.5241297819911779 1107 44-45 -0.6300008784435391 1107 46-47 -0.8468487264285329 1107 48-49 -0.7982435631721678 1107 50-51 -0.7972839879070648 1107 52-53 -0.7171436037818033 1107 54-55 -0.624522911966114 1107 56-57 -1.1021788041855984 1107 58-59 -0.7855251535241514 1107 60-61 -0.49163722655680786 1107 62-63 -0.5519513222075965 1107 64-65 -0.5730465118335104 1107 66-67 -0.5455643702622588 1107 68-69 -0.38929631741911663 1107 70-71 -0.6242865745732367 1107 72-73 -0.24866573115594193 1107 74-75 -0.336897749366031 1107 76-77 -0.05273256381055802 1107 78-79 -0.12526953649167538 1107 80-81 0.1691682264242651 1107 82-83 -0.040484648769162135 1107 84-85 -0.23353634456992367 1107 86-87 -0.3591155331280582 1107 88-89 -0.11483662063943356 1107 90-91 -0.026194768308872796 1107 92-93 0.3080124598230114 1107 94-95 0.5416390006057945 1107 96-97 0.9107081115329159 1107 98-99 0.8245220437573 1107 100-101 0.586003204106186 1108 1 0.05697562237879339 1108 2 0.04136729371718317 1108 3 0.014889416359306296 1108 4 0.0652099107571118 1108 5 0.06717197318079116 1108 6 0.09986745238584405 1108 7 -0.03124383226234073 1108 8 0.047610242592533325 1108 9 0.29918106107473363 1108 10-11 0.05049281895310287 1108 12-13 0.12342247268431095 1108 14-15 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50-51 -2.4753121363793156 2316 52-53 -2.553782802595581 2316 54-55 -1.8211368306321027 2316 56-57 -1.5491828635939484 2316 58-59 -1.4072685114084003 2316 60-61 -1.2797701417953888 2316 62-63 -0.8352567015313532 2316 64-65 -1.5014404932137104 2316 66-67 -2.72214863337026 2316 68-69 -3.0112049685773847 2316 70-71 -2.10888513068787 2316 72-73 -1.4458873456823014 2316 74-75 -1.160301332340822 2316 76-77 -1.2797045368774747 2316 78-79 -1.7838635713491122 2316 80-81 -2.167522018841783 2316 82-83 -1.7252096056227586 2316 84-85 -1.459412034304723 2316 86-87 -1.5919748636903002 2316 88-89 -2.0411060972917596 2316 90-91 -2.7781770722054198 2316 92-93 -2.6759006153611224 2316 94-95 -2.2142641569818977 2316 96-97 -2.4745637715950153 2316 98-99 -2.4659079618334996 2316 100-101 -1.5226746927799812 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 21.0 4 43.0 5 116.0 6 317.0 7 616.0 8 1215.0 9 1977.0 10 2827.0 11 3962.0 12 5169.0 13 6736.0 14 8592.0 15 10769.0 16 12559.0 17 14541.0 18 12411.0 19 9731.0 20 9476.0 21 10186.0 22 10828.0 23 12024.0 24 13343.0 25 15505.0 26 17628.0 27 20719.0 28 23573.0 29 27357.0 30 30790.0 31 34603.0 32 39257.0 33 44779.0 34 51913.0 35 54841.0 36 46142.0 37 29257.0 38 6441.0 39 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.805988230928634 22.03778098954406 16.04683907413024 18.109391705397066 2 18.335320901994795 25.194126734605376 35.27042226799653 21.200130095403296 3 21.903776180183353 28.40943740132671 30.310062812112506 19.37672360637743 4 14.765447406488944 19.98312888111959 34.64694304134102 30.60448067105045 5 12.593206156264502 40.76611141036456 31.76835072955732 14.872331703813618 6 29.100866086113996 37.94670004759832 16.019120633785995 16.93331323250169 7 25.848634731350362 37.338573074056505 19.662239989159158 17.15055220543397 8 27.632122772545564 33.027813537502546 18.981468934209637 20.35859475574226 9 27.602871526624973 17.08432992065697 19.880646669422102 35.43215188329595 10-11 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33.10282555553108 19.737637876911556 36-37 21.321944281855306 26.52367669618118 32.735871192131796 19.418507829831714 38-39 20.967695484754078 26.294238923645874 33.511805234385186 19.226260357214855 40-41 20.371712955748137 26.788351955118 33.35024426270606 19.489690826427804 42-43 20.355462579877447 27.54407287109929 32.359883629834464 19.740580919188798 44-45 20.575772692509332 27.534642320842817 31.431029999771326 20.45855498687653 46-47 20.770818458922065 28.11151510223855 30.496248472329146 20.621417966510236 48-49 21.522134986575875 27.870010417460676 29.84280644696835 20.765048148995096 50-51 21.389176379716 28.51647393018123 29.43625636691019 20.658093323192578 52-53 21.465667940482966 28.602003550424154 29.410843022467954 20.52148548662493 54-55 20.61111308734325 28.442836356121546 29.559294586761393 21.386755969773812 56-57 21.323597915145683 28.87501206905021 28.4204535252637 21.380936490540407 58-59 21.765323156431748 28.02391436360911 28.765923641054176 21.444838838904968 60-61 21.322742853077415 28.281257543303322 29.321672286295303 21.074327317323956 62-63 22.110036495886696 27.84062757002103 29.51500024138611 20.53433569270616 64-65 21.87376655672611 28.132840040723746 29.449246932590682 20.54414646995946 66-67 21.358618919809366 28.55876053083809 29.22743222447042 20.855188324882125 68-69 21.4663277690776 28.417426744169305 29.29360857328463 20.82263691346847 70-71 21.51204349888204 28.84816044447456 28.591791449285182 21.048004607358223 72-73 21.549182570192755 28.330169990505738 28.42324934171132 21.697398097590185 74-75 21.260077938599427 29.125253139522968 28.293862907003042 21.320806014874567 76-77 21.42917639964292 28.50365971199989 28.95263294299793 21.114530945359256 78-79 21.166013094038234 28.256104481277898 29.29354868763181 21.284333737052062 80-81 20.82423548423708 29.44874233705604 28.57009762154416 21.156924557162725 82-83 20.936151471391607 29.092179539843393 28.392416266828153 21.579252721936843 84-85 20.74386136089718 28.526599569568983 28.28614430534346 22.443394764190376 86-87 21.46362947247172 28.35922909811594 28.081680705008488 22.09546072440385 88-89 21.449625206454073 28.08825646889425 28.395883623427775 22.0662347012239 90-91 21.191677148580602 28.318446897988743 28.49471245674887 21.995163496681787 92-93 20.855466870837258 28.74689170602145 28.410484521204154 21.987156901937137 94-95 20.657471038514245 28.598059127335677 27.88467239092543 22.859797443224647 96-97 20.804480686230004 28.279207800039295 27.552188848762444 23.364122664968257 98-99 21.181810405209802 28.08070148395397 27.411605178974792 23.325882931861432 100-101 21.39312584326191 28.213330758552463 27.22043088619045 23.173112511995175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3629.0 1 2547.5 2 2042.5 3 2542.5 4 2037.0 5 1760.5 6 2701.5 7 4698.0 8 6157.5 9 6425.5 10 5184.5 11 3439.5 12 2829.0 13 2687.0 14 2623.5 15 2415.0 16 2171.0 17 2050.0 18 2004.0 19 1977.5 20 2005.5 21 2058.5 22 2113.5 23 2242.0 24 2433.0 25 2694.0 26 2988.0 27 3423.5 28 4005.0 29 4592.5 30 5445.5 31 6548.5 32 7901.0 33 9100.0 34 10048.0 35 10877.5 36 11748.0 37 12603.0 38 13096.5 39 13317.5 40 13537.5 41 14323.0 42 14928.0 43 15564.5 44 16487.0 45 17037.0 46 17548.5 47 19846.0 48 27945.5 49 29027.0 50 22625.0 51 18995.5 52 16116.5 53 15754.0 54 15784.0 55 15112.0 56 14364.5 57 13347.5 58 12534.5 59 11945.0 60 10662.0 61 9151.5 62 7835.5 63 6333.5 64 5038.5 65 4163.0 66 3330.0 67 2656.0 68 1959.5 69 1383.5 70 1086.0 71 898.5 72 756.5 73 671.5 74 603.0 75 485.5 76 355.0 77 244.0 78 157.5 79 125.5 80 101.0 81 65.5 82 48.0 83 37.0 84 18.5 85 8.5 86 6.5 87 4.5 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02862660071820584 2 0.00406531607832509 3 0.0047428687580459376 4 3.3877633986042414E-4 5 3.3877633986042414E-4 6 5.081645097906362E-4 7 0.0 8 0.0 9 0.002032658039162545 10-11 5.081645097906362E-4 12-13 9.316349346161664E-4 14-15 0.0016091876143370146 16-17 0.0023714343790229688 18-19 0.0 20-21 0.0 22-23 0.002202046209092757 24-25 5.928585947557422E-4 26-27 0.005589809607696999 28-29 0.006521444542313165 30-31 0.0013551053594416966 32-33 3.3877633986042414E-4 34-35 0.011687783725184633 36-37 0.014482688529033134 38-39 0.012026560065045058 40-41 0.002032658039162545 42-43 4.2347042482553023E-4 44-45 9.316349346161664E-4 46-47 8.469408496510604E-5 48-49 8.469408496510605E-4 50-51 5.081645097906362E-4 52-53 0.0013551053594416966 54-55 0.001863269869232333 56-57 0.0011857171895114844 58-59 5.928585947557422E-4 60-61 0.0031336811437089234 62-63 0.0033030693136391356 64-65 0.0069449149671386955 66-67 0.0017785757842672266 68-69 0.002456128463988075 70-71 0.0 72-73 5.928585947557422E-4 74-75 0.003980621993359984 76-77 0.002202046209092757 78-79 0.0025408225489531814 80-81 0.0032183752286740292 82-83 0.002964292973778711 84-85 0.0036418456534995595 86-87 0.0021173521241276507 88-89 0.005928585947557422 90-91 0.009739819770987194 92-93 6.775526797208483E-4 94-95 0.0011857171895114844 96-97 6.775526797208483E-4 98-99 0.001947963954197439 100-101 0.0031336811437089234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 590360.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.65220200697686 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98775787932237 32.29728552991474 2 12.910542378934215 11.271472078897437 3 4.949094538675147 6.481166236616202 4 2.406361308215796 4.2017187971203604 5 1.3727262027740268 2.9961260751881045 6 0.8603398981704433 2.2533438617758854 7 0.600637105251018 1.835339257591228 8 0.4232605920308404 1.478100549193829 9 0.33709508830416857 1.3243448601191943 >10 1.9147378078905868 15.62912060513817 >50 0.1611526902331363 4.841178184506948 >100 0.06461677904550785 5.28415846926417 >500 0.0062281232814947315 1.9554342936617168 >1k 0.0038925770509342077 3.344016935980413 >5k 0.0015570308203736829 4.807194265031598 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8754 1.4828240395690764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8177 1.3850870655193441 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5866 0.9936310048106239 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5494 0.930618605596585 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3438 0.5823565282200691 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2367 0.400941798224812 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2265 0.3836642048919303 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2245 0.3802764414933261 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2113 0.3579172030625381 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1832 0.3103191273121485 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 0.2722067890778508 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1407 0.23832915509180838 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1398 0.23680466156243649 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1008 0.17074327528965377 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 985 0.1668473473812589 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 915 0.15499017548614405 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 903 0.1529575174469815 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 877 0.14855342502879598 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 859 0.14550443797005216 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 858 0.14533504980012196 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 788 0.13347787790500712 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 754 0.1277186801273799 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 685 0.11603089640219526 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.234704248255302E-4 0.0 24-25 0.0 0.0 0.0 0.0014397994444068027 0.0 26-27 0.0 0.0 0.0 0.002286740294057863 0.0 28-29 0.0 0.0 0.0 0.004319398333220408 0.0 30-31 0.0 0.0 0.0 0.008723490751405922 0.0 32-33 0.0 0.0 0.0 0.013974524019242497 0.0 34-35 0.0 0.0 0.0 0.02134290941120672 0.0 36-37 0.0 0.0 0.0 0.032607222711565825 0.0 38-39 0.0 0.0 0.0 0.05090114506402873 0.0 40-41 0.0 0.0 0.0 0.07258283081509587 0.0 42-43 0.0 0.0 0.0 0.09485737516091877 0.0 44-45 0.0 0.0 0.0 0.11907988346093909 0.0 46-47 0.0 0.0 0.0 0.15515956365607425 0.0 48-49 0.0 0.0 0.0 0.19140863202113964 0.0 50-51 0.0 0.0 0.0 0.23121485195473948 0.0 52-53 0.0 0.0 0.0 0.272376177247781 0.0 54-55 0.0 0.0 0.0 0.31082729182193913 0.0 56-57 0.0 0.0 0.0 0.3621519073107934 0.0 58-59 0.0 0.0 0.0 0.4227081780608442 0.0 60-61 0.0 0.0 0.0 0.4930889626668473 0.0 62-63 0.0 0.0 0.0 0.5639779117826411 0.0 64-65 0.0 0.0 0.0 0.6324954265194118 0.0 66-67 0.0 0.0 0.0 0.7215936039027034 0.0 68-69 0.0 0.0 0.0 0.8188224134426452 0.0 70-71 0.0 0.0 0.0 0.9294328884070737 0.0 72-73 0.0 0.0 0.0 1.0502066535673147 0.0 74-75 0.0 0.0 0.0 1.1786875804593806 0.0 76-77 0.0 0.0 0.0 1.3158919981028525 0.0 78-79 0.0 0.0 0.0 1.4606341893082186 0.0 80-81 0.0 0.0 0.0 1.6243478555457687 0.0 82-83 0.0 0.0 0.0 1.8056778914560607 0.0 84-85 0.0 0.0 0.0 2.010637577071617 0.0 86-87 0.0 0.0 0.0 2.2146656277525576 0.0 88-89 0.0 0.0 0.0 2.4188630666034285 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCGT 40 4.491602E-4 29.689884 18-19 TAAGGTA 115 3.860516E-6 28.912916 4 CGATTCG 45 8.9390797E-4 26.393242 38-39 ACTTGAC 225 1.0186341E-10 25.331074 8 CTTGACA 230 1.3460522E-10 24.7804 9 GTAAGGT 220 2.1627784E-9 23.749893 3 TAGTTCC 165 2.42665E-6 23.030201 4 GAGTACT 6590 0.0 22.522652 12-13 GTAGTCT 235 4.642061E-9 22.232061 9 ATGCACT 305 9.094947E-12 21.80318 4 AGTACTT 6720 0.0 21.274145 12-13 GTACTTT 7060 0.0 21.056904 14-15 TTAGGGT 325 2.1827873E-11 20.461447 4 GGCCATT 325 2.1827873E-11 20.459713 7 GTCGGGA 235 1.1285738E-7 20.214388 2 TACGATT 60 0.0047567 19.796608 36-37 ACTTTTT 7650 0.0 19.74501 16-17 CATGCAC 290 2.4319888E-9 19.655085 3 TACTTTT 7370 0.0 19.333418 14-15 GACTTGA 375 9.094947E-12 18.998306 7 >>END_MODULE