##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936388_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 558338 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13885137676461 33.0 31.0 34.0 31.0 34.0 2 32.21082749159112 33.0 31.0 34.0 31.0 34.0 3 32.163205800070926 34.0 31.0 34.0 30.0 34.0 4 35.82849814986621 37.0 35.0 37.0 35.0 37.0 5 35.8784714635221 37.0 35.0 37.0 35.0 37.0 6 35.989724862001154 37.0 36.0 37.0 35.0 37.0 7 35.905184673083326 37.0 36.0 37.0 35.0 37.0 8 35.90099008127693 37.0 36.0 37.0 35.0 37.0 9 37.520168786648945 39.0 38.0 39.0 35.0 39.0 10-11 37.44391837918967 39.0 37.0 39.0 35.0 39.0 12-13 37.36240055307 39.0 37.0 39.0 35.0 39.0 14-15 38.46331523199209 40.0 38.0 41.0 34.5 41.0 16-17 38.42708180349537 40.0 38.0 41.0 34.5 41.0 18-19 38.410539852204224 40.0 38.0 41.0 34.0 41.0 20-21 38.42149199946986 40.0 38.0 41.0 34.0 41.0 22-23 38.38218874588511 40.0 38.0 41.0 34.0 41.0 24-25 38.42485376241632 40.0 38.0 41.0 35.0 41.0 26-27 38.31325111312502 40.0 38.0 41.0 34.0 41.0 28-29 38.15457214088957 40.0 38.0 41.0 34.0 41.0 30-31 37.85633612614581 40.0 37.0 41.0 34.0 41.0 32-33 37.56441976007365 40.0 35.5 41.0 33.5 41.0 34-35 37.33820911347607 39.0 35.0 41.0 33.0 41.0 36-37 37.10785581493647 39.0 35.0 41.0 33.0 41.0 38-39 36.860082960500634 39.0 35.0 41.0 33.0 41.0 40-41 36.555305209389296 39.0 35.0 40.5 31.5 41.0 42-43 36.23654488858004 38.0 35.0 40.0 31.0 41.0 44-45 35.97595811139489 38.5 35.0 40.0 30.5 41.0 46-47 35.58890224201111 38.0 35.0 40.0 29.5 41.0 48-49 35.22133456794988 38.0 35.0 40.0 27.5 41.0 50-51 34.176665389065406 37.5 34.0 39.5 25.5 40.5 52-53 34.268633874104935 37.5 34.0 39.5 26.0 40.5 54-55 34.651015155694225 38.0 35.0 40.0 26.0 41.0 56-57 34.48059687859325 38.0 35.0 40.0 25.5 41.0 58-59 34.25967335198392 37.0 35.0 40.0 25.5 41.0 60-61 33.94992638867496 37.0 34.0 40.0 24.5 41.0 62-63 33.58534077924125 36.0 34.0 40.0 23.0 41.0 64-65 33.18704530230792 35.5 33.5 39.0 22.0 41.0 66-67 32.81453886355577 35.0 33.0 39.0 20.5 41.0 68-69 32.39234119834222 35.0 33.0 38.5 18.5 40.0 70-71 31.97188083204081 35.0 33.0 37.5 14.5 40.0 72-73 31.565170201562495 35.0 32.5 37.0 9.5 39.0 74-75 31.172814674981822 35.0 32.0 36.5 6.0 39.0 76-77 30.72029487514731 35.0 31.5 36.0 2.0 38.5 78-79 30.29099398572191 35.0 31.0 35.0 2.0 37.0 80-81 29.90251962073153 34.5 31.0 35.0 2.0 37.0 82-83 29.531505109808037 34.0 30.5 35.0 2.0 36.5 84-85 29.19402404994824 34.0 30.0 35.0 2.0 36.0 86-87 28.871397791302044 34.0 29.5 35.0 2.0 36.0 88-89 28.548229746139434 34.0 29.0 35.0 2.0 35.0 90-91 28.204046652744395 34.0 29.0 35.0 2.0 35.0 92-93 27.846457701249065 34.0 29.0 35.0 2.0 35.0 94-95 27.41766277774395 34.0 27.0 35.0 2.0 35.0 96-97 26.890254648617862 34.0 26.0 35.0 2.0 35.0 98-99 26.25858171931697 33.0 24.5 35.0 2.0 35.0 100-101 23.902552754782945 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.569312224227275 1101 2 0.5264128355768278 1101 3 0.5865032928119902 1101 4 0.6407857469890672 1101 5 0.5390113562685812 1101 6 0.5034311218973855 1101 7 0.7004909032105431 1101 8 0.403674341752442 1101 9 0.6949585517529542 1101 10-11 0.714652475742902 1101 12-13 0.7240239599628566 1101 14-15 0.8888760244569411 1101 16-17 0.9363527343470395 1101 18-19 0.8852473565926573 1101 20-21 0.8430389682309496 1101 22-23 0.7653530797214927 1101 24-25 0.765074326508703 1101 26-27 0.7578786571019549 1101 28-29 0.7863398223323514 1101 30-31 0.8557385546867167 1101 32-33 0.8918637017766144 1101 34-35 0.9299251487228233 1101 36-37 1.0082899786410664 1101 38-39 0.9623713970318235 1101 40-41 0.9977134566820993 1101 42-43 1.2467175690223655 1101 44-45 0.9809885480799991 1101 46-47 1.0452574464433297 1101 48-49 1.003452568945285 1101 50-51 0.8572528596638804 1101 52-53 0.9366552611659458 1101 54-55 1.050185016467978 1101 56-57 1.2592126751489587 1101 58-59 1.1622749217378257 1101 60-61 0.92723608331967 1101 62-63 0.9178737936465211 1101 64-65 0.6529219339820003 1101 66-67 0.5983407421076592 1101 68-69 0.6068957171102269 1101 70-71 0.6172403815735237 1101 72-73 0.8167962249118652 1101 74-75 0.44531942707363825 1101 76-77 0.5804896637632702 1101 78-79 0.5534675194297698 1101 80-81 0.6678216984821468 1101 82-83 0.5843252527729561 1101 84-85 0.4743177036227557 1101 86-87 0.3780652227559038 1101 88-89 0.5998939243274606 1101 90-91 0.6478099464973113 1101 92-93 0.5522675242070569 1101 94-95 0.7755149960606573 1101 96-97 0.4623440349164376 1101 98-99 0.48005385135207845 1101 100-101 0.7651694759925114 1102 1 0.16594697557054872 1102 2 0.24101981924210492 1102 3 0.41777626982487703 1102 4 0.2086832408955317 1102 5 0.28049723676791416 1102 6 0.15785684807198663 1102 7 0.1901015901306451 1102 8 0.1322392295101551 1102 9 0.22374294152389496 1102 10-11 0.3864262674718475 1102 12-13 0.2832322714133255 1102 14-15 0.34839271603641464 1102 16-17 0.2703189875958003 1102 18-19 0.5208201491161191 1102 20-21 0.3566837789609636 1102 22-23 0.2809000167930904 1102 24-25 0.3737581224122337 1102 26-27 0.10775543971416113 1102 28-29 0.13825002850264667 1102 30-31 0.09885723390405587 1102 32-33 0.3394660126076161 1102 34-35 0.2593936306576694 1102 36-37 0.059041402710882096 1102 38-39 -0.07827350796285515 1102 40-41 0.044222970680401374 1102 42-43 0.16662290036266825 1102 44-45 0.07151292896313066 1102 46-47 0.22702329300738455 1102 48-49 0.23267394278549602 1102 50-51 0.03194885610876241 1102 52-53 -0.11061173677369851 1102 54-55 -0.06440378758271237 1102 56-57 -0.09102096656450698 1102 58-59 -0.10888076309498018 1102 60-61 -0.41471910056376515 1102 62-63 -0.35888727235221296 1102 64-65 -0.0797434668448389 1102 66-67 -0.17800959926510274 1102 68-69 -0.11530293894493582 1102 70-71 -0.12257903425485495 1102 72-73 -0.10744191457291308 1102 74-75 -0.32825347318087594 1102 76-77 -0.23280569437513776 1102 78-79 -0.2727757642126214 1102 80-81 0.03565590039706379 1102 82-83 -0.16534818748344193 1102 84-85 -0.030509994030055054 1102 86-87 -0.36034022304864877 1102 88-89 -0.30019226051208747 1102 90-91 -0.22397663829375603 1102 92-93 -0.317472170644745 1102 94-95 -0.21067186344458833 1102 96-97 -0.06247450559716938 1102 98-99 0.04095892683114144 1102 100-101 0.7578437646309304 1103 1 0.20510208385434225 1103 2 0.27475856502721285 1103 3 0.21764460854632972 1103 4 0.34151991138602256 1103 5 0.24549836653466173 1103 6 0.28145333015215357 1103 7 0.1850306755410145 1103 8 0.2331404357534197 1103 9 0.3595170055555954 1103 10-11 0.3453049305232696 1103 12-13 0.3683688862845358 1103 14-15 0.44879204512877635 1103 16-17 0.48834658866647374 1103 18-19 0.6080479187827592 1103 20-21 0.6166941641245174 1103 22-23 0.45397702343264257 1103 24-25 0.3528205029176732 1103 26-27 0.3865058307872715 1103 28-29 0.3810173486959201 1103 30-31 0.3396148728653685 1103 32-33 0.33920421089037234 1103 34-35 0.29504972096948734 1103 36-37 0.4104021198520442 1103 38-39 0.3619781258536676 1103 40-41 0.3673970938075968 1103 42-43 0.25915296953915856 1103 44-45 0.27839060129601023 1103 46-47 0.44241332325037064 1103 48-49 0.26928598957521643 1103 50-51 0.22167905911914687 1103 52-53 0.5480042555091273 1103 54-55 0.4736258992111857 1103 56-57 0.5967908056049964 1103 58-59 0.5298285218495735 1103 60-61 0.6450672866159906 1103 62-63 0.4775301069365554 1103 64-65 0.479535611613116 1103 66-67 0.5889781818730029 1103 68-69 0.7392504555696107 1103 70-71 0.8228304192856584 1103 72-73 0.6547628252331101 1103 74-75 0.22926034473548285 1103 76-77 0.3161577446348396 1103 78-79 0.34700015089844527 1103 80-81 0.4165057022533567 1103 82-83 -3.131647142957661E-4 1103 84-85 0.2334969409996681 1103 86-87 0.03559848276008992 1103 88-89 0.14181811596095528 1103 90-91 0.0928414605695238 1103 92-93 0.028610145898518624 1103 94-95 0.031475171352226994 1103 96-97 -0.033188561633600955 1103 98-99 0.5558294816335199 1103 100-101 0.7771269276301958 1104 1 0.12409346286501233 1104 2 0.278389027014903 1104 3 0.5552949544287671 1104 4 0.41645603611227244 1104 5 0.31637167622979945 1104 6 0.23409003192848843 1104 7 0.22298649297070483 1104 8 0.2592945359376557 1104 9 0.46440063958069544 1104 10-11 0.33560414490084867 1104 12-13 0.3953398947317126 1104 14-15 0.5486916696985986 1104 16-17 0.48631300852255777 1104 18-19 0.3085630489666471 1104 20-21 0.2901665891253842 1104 22-23 0.19719299138203183 1104 24-25 0.44251746471417874 1104 26-27 0.18623077603703564 1104 28-29 0.2129884440482357 1104 30-31 0.12875260012228296 1104 32-33 0.20418948047010588 1104 34-35 0.3318898937368928 1104 36-37 0.41554364486334094 1104 38-39 0.1676158791821294 1104 40-41 0.22882685707801187 1104 42-43 0.5134091484942047 1104 44-45 0.431752091892875 1104 46-47 0.6096839806008063 1104 48-49 0.3993406094521603 1104 50-51 0.6298561804734817 1104 52-53 0.5560549298929658 1104 54-55 0.5581672139120641 1104 56-57 0.4059894968136746 1104 58-59 0.3544974132876746 1104 60-61 0.34836012215671985 1104 62-63 0.5065835796657225 1104 64-65 0.4366989845751945 1104 66-67 0.1822295636646416 1104 68-69 0.3331275543286054 1104 70-71 0.27129241567040197 1104 72-73 0.5024235562791048 1104 74-75 0.4736897256233057 1104 76-77 0.5369999857444405 1104 78-79 0.3131734136648099 1104 80-81 0.5461192785734461 1104 82-83 0.47516003374226656 1104 84-85 0.7744227413959557 1104 86-87 0.7173877291187658 1104 88-89 0.42925467521287786 1104 90-91 0.4613746322593073 1104 92-93 0.49045010140308776 1104 94-95 0.6144402441787307 1104 96-97 0.6318453748213635 1104 98-99 0.437819913378231 1104 100-101 1.2085164103708372 1105 1 -0.11365481632016383 1105 2 -0.036597341522167426 1105 3 0.20540013758938613 1105 4 0.2176083582680306 1105 5 0.09046480052447237 1105 6 0.08865800268322488 1105 7 0.1557516770011773 1105 8 -0.09317549430107164 1105 9 0.001320043413066685 1105 10-11 0.07967714819483263 1105 12-13 0.1834753715582238 1105 14-15 0.2403733870569127 1105 16-17 0.25717738748409147 1105 18-19 0.12155334098439852 1105 20-21 0.25668423677667107 1105 22-23 0.11133995480484771 1105 24-25 -0.20160406704564338 1105 26-27 -0.0871332767308104 1105 28-29 -0.056469296127609425 1105 30-31 -0.19781078331897106 1105 32-33 -0.11415839995758859 1105 34-35 0.031820305748148314 1105 36-37 -0.10242218834981287 1105 38-39 0.06726610797553434 1105 40-41 -0.04516211414140514 1105 42-43 -0.33567991268598973 1105 44-45 -0.19578048741797716 1105 46-47 0.005534338956053375 1105 48-49 0.005219649961986761 1105 50-51 0.2935310214397404 1105 52-53 0.4703873086805359 1105 54-55 0.25032734869300555 1105 56-57 0.36691149188299477 1105 58-59 0.4776097818659508 1105 60-61 0.5372331401008026 1105 62-63 0.7593699605638236 1105 64-65 0.5446988563867947 1105 66-67 0.4502646415047522 1105 68-69 0.5440890360033919 1105 70-71 0.41852137159877145 1105 72-73 0.301532875804714 1105 74-75 0.3482104497962091 1105 76-77 0.1387998882296948 1105 78-79 0.4329151140381562 1105 80-81 0.45824841923045767 1105 82-83 0.4911194895825055 1105 84-85 0.687940208210037 1105 86-87 0.4969586184486836 1105 88-89 0.677509825067407 1105 90-91 0.7169410990894107 1105 92-93 0.8695856079876236 1105 94-95 0.8369581390505054 1105 96-97 1.0099942885391258 1105 98-99 0.9403951267775774 1105 100-101 1.3671152195921614 1106 1 0.19594510711015545 1106 2 0.15564519293418755 1106 3 0.21350216668586341 1106 4 0.25067688813020794 1106 5 0.2815085409532614 1106 6 0.3273472255009864 1106 7 0.34825741972706226 1106 8 0.5101026450557455 1106 9 0.3685649706718763 1106 10-11 0.5720309479651249 1106 12-13 0.44127949674965095 1106 14-15 0.4467753300124926 1106 16-17 0.2816605421548459 1106 18-19 0.4151155059920555 1106 20-21 0.1910400943570707 1106 22-23 0.0745075466884586 1106 24-25 -0.10911627033814142 1106 26-27 0.12102617962447937 1106 28-29 0.20085746497498747 1106 30-31 0.17615193681885444 1106 32-33 0.2373873504252586 1106 34-35 0.3401987035276264 1106 36-37 0.1433054628753041 1106 38-39 0.23735966655134177 1106 40-41 0.18209549497038324 1106 42-43 0.3942002079112541 1106 44-45 0.34058173501694 1106 46-47 0.5149787302271136 1106 48-49 0.47737101864498754 1106 50-51 0.22643923353775364 1106 52-53 0.22111472062540827 1106 54-55 0.46875671316467304 1106 56-57 0.42586057304685454 1106 58-59 0.35028637068678137 1106 60-61 0.3368550773136647 1106 62-63 0.4147394092621397 1106 64-65 0.25004796626428316 1106 66-67 0.5438291993148638 1106 68-69 0.5154495834765882 1106 70-71 0.5382919018437917 1106 72-73 0.31143070314009336 1106 74-75 0.26200017031688105 1106 76-77 0.18932271556507274 1106 78-79 0.4559027671775162 1106 80-81 0.44328622550917274 1106 82-83 0.7003772422624444 1106 84-85 0.8360223770461808 1106 86-87 1.1152133083414881 1106 88-89 1.2293726217749104 1106 90-91 1.3146619547555716 1106 92-93 1.4279197389217728 1106 94-95 1.398553659724321 1106 96-97 1.4431740837152383 1106 98-99 1.7522502665172404 1106 100-101 2.2075775090255476 1107 1 0.07438160228700497 1107 2 0.31142292605315447 1107 3 0.19831928887236216 1107 4 0.10347029649033601 1107 5 0.1899339830754485 1107 6 0.2983530534752674 1107 7 0.26202349689747706 1107 8 0.2423474199753599 1107 9 0.2810800430191378 1107 10-11 0.3130804857464753 1107 12-13 0.3243155699007758 1107 14-15 0.05857948998034601 1107 16-17 0.23531809842494056 1107 18-19 0.4084409682107122 1107 20-21 0.3035702390516093 1107 22-23 0.4220306576209367 1107 24-25 0.20359725458147437 1107 26-27 0.21528863943154875 1107 28-29 0.3212654231743528 1107 30-31 0.3029832270117794 1107 32-33 0.1876184597500128 1107 34-35 0.26608043584274554 1107 36-37 0.2537782321679103 1107 38-39 0.3765854503134065 1107 40-41 0.5295708768031844 1107 42-43 0.6846418276861712 1107 44-45 0.6976682502902491 1107 46-47 0.7687518411595846 1107 48-49 0.7353066094810004 1107 50-51 0.6089402383609084 1107 52-53 0.4917738846125417 1107 54-55 0.42292672924184416 1107 56-57 0.6587182900886575 1107 58-59 0.6570438063780983 1107 60-61 0.6037059615580418 1107 62-63 0.5150006632814339 1107 64-65 0.7591592001871135 1107 66-67 0.43979232230521603 1107 68-69 0.42744214778527834 1107 70-71 0.6833305875351172 1107 72-73 0.41399551423333847 1107 74-75 0.2593022201561084 1107 76-77 0.3139634912885434 1107 78-79 0.40893230897815513 1107 80-81 0.3025657673098152 1107 82-83 0.44358966187659377 1107 84-85 0.519927622223026 1107 86-87 0.6356765318141555 1107 88-89 0.7153478026430768 1107 90-91 0.591759523083546 1107 92-93 0.5306478337770777 1107 94-95 0.3636159587596879 1107 96-97 0.744261804776329 1107 98-99 1.1805887914368576 1107 100-101 1.7941832164210467 1108 1 -0.037984925623064214 1108 2 0.022886395880178156 1108 3 -0.029802866047354826 1108 4 0.11498744459502319 1108 5 -0.06271967099436182 1108 6 0.0039250691998447 1108 7 0.12699105311494918 1108 8 0.3002673340901154 1108 9 -0.05551696468000955 1108 10-11 0.0788550580305909 1108 12-13 0.032496507797112884 1108 14-15 0.4309291250206826 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-3.8674635541349787 2316 54-55 -3.2906582229375623 2316 56-57 -3.186339906186369 2316 58-59 -3.745804883306832 2316 60-61 -3.8000159290761104 2316 62-63 -3.45213359393275 2316 64-65 -4.414636039326773 2316 66-67 -3.4276788877458415 2316 68-69 -4.198463156140022 2316 70-71 -4.122872734737673 2316 72-73 -2.7185538216202936 2316 74-75 -2.692427745018584 2316 76-77 -3.2918299401857247 2316 78-79 -3.952778343046127 2316 80-81 -4.057547440625015 2316 82-83 -3.582734778504328 2316 84-85 -3.7092876269474324 2316 86-87 -4.585071236386948 2316 88-89 -5.289719331100489 2316 90-91 -5.199363105148585 2316 92-93 -4.4358931604392495 2316 94-95 -3.430629646984947 2316 96-97 -4.6877389554221445 2316 98-99 -5.962608459521096 2316 100-101 -6.49892209161343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1102.0 3 348.0 4 305.0 5 288.0 6 279.0 7 367.0 8 409.0 9 544.0 10 643.0 11 929.0 12 1586.0 13 2344.0 14 2551.0 15 3591.0 16 5294.0 17 8417.0 18 6334.0 19 3602.0 20 3377.0 21 3477.0 22 3801.0 23 3972.0 24 4233.0 25 4705.0 26 5417.0 27 6154.0 28 7393.0 29 9047.0 30 11195.0 31 14477.0 32 19392.0 33 27005.0 34 41786.0 35 71291.0 36 88697.0 37 108076.0 38 76156.0 39 9754.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.5789784967435 18.36576684433707 14.112968266656154 20.942286392263274 2 15.721029389371097 21.156764213222978 39.37065004165211 23.751556355753813 3 19.319285071990773 28.322340962910154 29.40103821475784 22.957335750341233 4 12.084429787241666 17.116862132527466 36.48849327976011 34.31021480047077 5 12.777799672534062 38.77497644323267 32.34460594812853 16.102617936104732 6 29.04070715286412 35.88130753051872 18.118821459667277 16.959163856949882 7 26.816197188147488 36.123737283214204 19.998422724242012 17.0616428043963 8 27.080369142549948 31.212436161634262 20.121494489741064 21.585700206074723 9 28.34943000055557 16.605882258061623 20.322124509169633 34.72256323221317 10-11 24.51796730447872 26.935858895842944 25.53144782291195 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20.60689427888766 26.95560594499909 31.949623691158084 20.487876084955168 38-39 20.61928333009428 26.705809968108003 32.10774677340918 20.567159928388538 40-41 19.870933768927383 27.320183121446917 32.60892537944326 20.19995773018244 42-43 19.802643421133936 27.989027984371234 31.437260614028094 20.771067980466732 44-45 19.517424064677787 28.298679829565298 31.111855384830307 21.07204072092661 46-47 19.87124206009122 28.329970653792664 30.347220169987704 21.451567116128416 48-49 20.438859570489683 28.37685720093815 29.76625977126574 21.418023457306433 50-51 20.520944527628725 28.744546255632848 29.657264853169803 21.077244363568628 52-53 20.310851689929198 28.716706935058934 29.71404366896695 21.258397706044914 54-55 19.74167907704404 28.561091743061755 30.010844714612066 21.68638446528214 56-57 20.423213802762916 29.200480725314915 28.83187688846779 21.54442858345438 58-59 20.443418353045693 28.361616022309725 29.30283000027762 21.892135624366965 60-61 20.238360705947464 28.24891391664361 29.89771875478562 21.615006622623312 62-63 20.962734170776418 28.380705554903805 29.721677190676065 20.93488308364371 64-65 20.5515689967215 28.542656776538077 29.943609073055665 20.962165153684754 66-67 20.309957561282708 28.801372653848183 29.524415919130913 21.364253865738192 68-69 20.14260664602136 28.94814613151263 29.870826534651336 21.03842068781467 70-71 20.342757968491412 28.77298844398593 29.314519884511505 21.569733703011153 72-73 20.17406678919593 28.690835742717212 29.141811498403897 21.993285969682965 74-75 20.016354357549602 29.08120037043605 29.29534751666335 21.607097755350992 76-77 20.256747412282746 28.47151809162794 29.66263834300107 21.60909615308824 78-79 19.98681560022607 28.430205531536423 30.058371422096947 21.52460744614056 80-81 19.98515080707782 29.283808558258006 29.482633698711847 21.24840693595233 82-83 19.85282528174009 28.964600692081188 29.19269732552891 21.98987670064981 84-85 19.964518746317093 28.450645952253446 28.917132197670526 22.667703103758935 86-87 20.338635464036436 28.296577824186098 29.364407007337967 22.000379704439496 88-89 20.591928042297717 27.89722170480434 29.176398102907253 22.334452149990685 90-91 20.050402286909737 28.221787973663282 29.520443912679024 22.20736582674796 92-93 20.142846907521182 28.48304644539576 29.319529276899104 22.054577370183956 94-95 19.904759942931197 28.26141667121939 29.19456144703268 22.639261938816727 96-97 20.000125385336005 27.9634376360207 28.766709835046633 23.26972714359666 98-99 20.144209668317764 28.048511774137204 28.522791397579784 23.28448715996525 100-101 20.582405485846948 27.68602554652016 28.954663480442427 22.776905487190465 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2663.0 1 1904.0 2 2162.5 3 2816.5 4 1932.5 5 1661.0 6 2856.5 7 5121.5 8 7300.5 9 7528.0 10 5222.0 11 3126.0 12 2725.0 13 2734.5 14 2603.5 15 2311.5 16 1995.0 17 1778.0 18 1672.0 19 1635.0 20 1618.0 21 1663.5 22 1758.0 23 1850.5 24 2020.5 25 2292.0 26 2638.5 27 3011.0 28 3451.0 29 4149.0 30 5019.0 31 5951.0 32 7409.0 33 8614.5 34 9615.5 35 10374.0 36 11081.5 37 11850.0 38 12121.0 39 12307.5 40 12372.0 41 13015.0 42 13602.5 43 14266.0 44 15158.0 45 15962.5 46 16211.5 47 17898.5 48 28409.0 49 28505.0 50 19246.0 51 16923.5 52 15042.0 53 14996.5 54 15239.0 55 14993.0 56 14260.5 57 13025.0 58 12208.5 59 11495.0 60 10272.5 61 8842.0 62 7763.5 63 6267.0 64 4757.0 65 4027.0 66 3252.0 67 2508.5 68 1824.0 69 1272.0 70 1013.5 71 865.5 72 749.0 73 719.0 74 606.5 75 477.5 76 345.0 77 213.0 78 150.5 79 115.0 80 78.5 81 54.5 82 40.0 83 24.0 84 16.5 85 12.5 86 7.5 87 4.5 88 3.5 89 1.5 90 1.0 91 2.5 92 4.0 93 2.0 94 0.5 95 0.0 96 0.5 97 1.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06734272071755817 2 0.025611726230347926 3 0.012895414605489864 4 0.017731195082548563 5 0.020059533830762012 6 0.02794006497856137 7 0.07414863398156672 8 0.051581658421959456 9 0.06358155812429031 10-11 0.05561147548617504 12-13 0.03805938338425828 14-15 0.0342982207909904 16-17 0.045133951119214526 18-19 0.0389548982874173 20-21 0.016835680179389544 22-23 0.011910348212014945 24-25 0.019790879359814308 26-27 0.023193835991818575 28-29 0.005910398360849521 30-31 0.0012537208644226258 32-33 0.008149185618747067 34-35 0.010567075857276417 36-37 0.0035820596126360737 38-39 0.008507391580010674 40-41 0.0035820596126360737 42-43 0.0015223753353703312 44-45 1.7910298063180367E-4 46-47 6.268604322113129E-4 48-49 8.059634128431165E-4 50-51 0.0010746178837908221 52-53 1.7910298063180367E-4 54-55 2.6865447094770554E-4 56-57 0.002686544709477055 58-59 0.0029551991804247606 60-61 0.0043880230254791895 62-63 0.0020596842772657425 64-65 6.268604322113129E-4 66-67 6.268604322113129E-4 68-69 0.0034029566320042696 70-71 0.0025074417288452515 72-73 0.01862670998570758 74-75 0.013790929508648882 76-77 0.022208769598343656 78-79 0.018358055514759875 80-81 0.010477524366960514 82-83 0.0039402655738996805 84-85 0.002686544709477055 86-87 0.001611926825686233 88-89 0.0017910298063180368 90-91 0.00537308941895411 92-93 0.008596943070326577 94-95 0.011552142250751338 96-97 0.010567075857276417 98-99 0.022029666617711854 100-101 0.018178952534128073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 558338.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.97236921835686 #Duplication Level Percentage of deduplicated Percentage of total 1 72.16378775734519 30.288851439464327 2 13.786306664470185 11.572879069572728 3 5.287599694365643 6.65799259952357 4 2.5341481568060313 4.25456808365925 5 1.4728437843409854 3.0909371558660905 6 0.9760806814207594 2.4581051248498484 7 0.6580246516437418 1.933319754350025 8 0.47429531267648584 1.5925838385754783 9 0.3464644208532347 1.3087739333768529 >10 2.036436418406376 16.065405403878504 >50 0.17143666083882342 4.820874003315921 >100 0.07971804727248014 5.773751706541804 >500 0.006000283128036139 1.653369639046165 >1k 0.005571691476033557 4.480521988872031 >5k 0.001285774956007744 4.0480662591073715 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9939 1.7801045244994966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7469 1.3377201623389416 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5095 0.9125296863190397 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4339 0.7771278329613962 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2777 0.4973689772145188 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2569 0.46011555724310366 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2032 0.36393725664382504 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2014 0.3607134029924526 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1990 0.3564149314572893 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1506 0.2697290888314963 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1426 0.25540085038095206 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1364 0.24429646558178023 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1262 0.22602796155733623 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1224 0.2192220482933277 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1217 0.21796832742890507 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1187 0.21259523800995095 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 909 0.16280460939430955 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 817 0.1463271351761836 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 794 0.1422077666216521 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 765 0.13701378018332983 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 755 0.13522275037701179 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 751 0.13450633845448456 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 694 0.12429746855847175 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 566 0.10137228703760087 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 565 0.10119318405696906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.955149031590184E-5 0.0 22-23 0.0 0.0 0.0 1.7910298063180367E-4 0.0 24-25 0.0 0.0 0.0 0.0012537208644226258 0.0 26-27 0.0 0.0 0.0 0.0024178902385293497 0.0 28-29 0.0 0.0 0.0 0.0029551991804247606 0.0 30-31 0.0 0.0 0.0 0.006268604322113128 0.0 32-33 0.0 0.0 0.0 0.010029766915381006 0.0 34-35 0.0 0.0 0.0 0.01647747421812594 0.0 36-37 0.0 0.0 0.0 0.027044550075402355 0.0 38-39 0.0 0.0 0.0 0.04271606088068518 0.0 40-41 0.0 0.0 0.0 0.061074116395445056 0.0 42-43 0.0 0.0 0.0 0.08077544426494346 0.0 44-45 0.0 0.0 0.0 0.10370062578581432 0.0 46-47 0.0 0.0 0.0 0.13755108912522523 0.0 48-49 0.0 0.0 0.0 0.1700582801098976 0.0 50-51 0.0 0.0 0.0 0.20605797921689012 0.0 52-53 0.0 0.0 0.0 0.24241588428514627 0.0 54-55 0.0 0.0 0.0 0.2786842378630865 0.0 56-57 0.0 0.0 0.0 0.3284748664787279 0.0 58-59 0.0 0.0 0.0 0.3885639164806981 0.0 60-61 0.0 0.0 0.0 0.4536678499403587 0.0 62-63 0.0 0.0 0.0 0.5162643416711741 0.0 64-65 0.0 0.0 0.0 0.5837861653693641 0.0 66-67 0.0 0.0 0.0 0.6689496326597867 0.0 68-69 0.0 0.0 0.0 0.7643219698462222 0.0 70-71 0.0 0.0 0.0 0.8727688246187794 0.0 72-73 0.0 0.0 0.0 0.978887340643123 0.0 74-75 0.0 0.0 0.0 1.0989758891567474 0.0 76-77 0.0 0.0 0.0 1.22237784281206 0.0 78-79 0.0 0.0 0.0 1.3704064563042457 0.0 80-81 0.0 0.0 0.0 1.5416289057882502 0.0 82-83 0.0 0.0 0.0 1.7162543119042586 0.0 84-85 0.0 0.0 0.0 1.9133571420895588 0.0 86-87 0.0 0.0 0.0 2.1277434099058277 0.0 88-89 0.0 0.0 0.0 2.3480400760829463 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTAC 20 0.0020710973 71.27097 1 GCTTATG 65 1.325013E-4 36.549217 1 TAGATAG 125 2.1433334E-7 30.411674 5 GTTCCGT 65 0.0058305813 29.241991 6 ATGGGAG 1080 0.0 28.158958 5 CCCTATA 120 5.362883E-6 27.716488 2 GTGTAAG 120 5.362883E-6 27.716488 1 TGTTCCG 70 0.008372243 27.15328 5 GGTAAGG 140 5.7822217E-7 27.150846 1 TAAGGTG 125 7.3651318E-6 26.60783 3 GTACATG 6265 0.0 26.468283 1 ATGGGAT 1285 0.0 26.255213 5 TACATGG 6255 0.0 25.902905 2 TAGACAC 95 0.0012230239 25.009598 5 ACATGGG 6275 0.0 24.684553 3 CATGGGA 4235 0.0 24.570395 4 GAGTACT 5390 0.0 24.512875 12-13 ATAGATC 175 1.4790749E-7 24.43576 3 GTATCAA 9880 0.0 24.093628 1 TGGGATG 755 0.0 23.916464 6 >>END_MODULE