##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936388_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 558338 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.961791961141817 31.0 31.0 33.0 28.0 34.0 2 31.071904473634252 31.0 31.0 34.0 28.0 34.0 3 31.03516328818744 31.0 31.0 34.0 27.0 34.0 4 34.91151954550828 35.0 35.0 37.0 32.0 37.0 5 34.70990152918125 35.0 35.0 37.0 32.0 37.0 6 34.89869935415465 35.0 35.0 37.0 32.0 37.0 7 34.81257768591785 35.0 35.0 37.0 32.0 37.0 8 34.79841601323929 35.0 35.0 37.0 32.0 37.0 9 36.189478416299806 38.0 35.0 39.0 32.0 39.0 10-11 36.03699551167931 37.0 35.0 39.0 30.5 39.0 12-13 35.871072719392195 37.0 35.0 39.0 30.0 39.0 14-15 36.53878385494092 38.0 35.0 40.0 30.0 41.0 16-17 36.41238461290473 38.0 35.0 40.0 30.0 41.0 18-19 36.684902335144656 38.0 35.0 40.0 31.0 41.0 20-21 36.731779853780324 38.5 35.0 40.0 31.0 41.0 22-23 36.572728347345155 38.0 35.0 40.0 30.0 41.0 24-25 36.308143096117405 38.0 35.0 40.0 30.0 41.0 26-27 35.87829862914579 38.0 34.0 40.0 29.0 41.0 28-29 35.577390397931 38.0 34.0 40.0 27.5 41.0 30-31 35.2696440149157 37.5 34.0 40.0 27.0 41.0 32-33 34.850810799193326 37.0 33.0 40.0 27.0 41.0 34-35 34.477804663125205 37.0 33.0 40.0 25.5 41.0 36-37 34.05242792000544 36.0 33.0 39.5 24.0 41.0 38-39 33.660213884779466 36.0 33.0 39.0 23.5 40.5 40-41 33.23755323836099 35.5 32.0 39.0 22.0 40.0 42-43 32.73790069814342 35.0 31.0 39.0 20.0 40.0 44-45 32.16574816688099 35.0 31.0 39.0 16.5 40.0 46-47 31.398561444859567 35.0 30.0 39.0 11.0 40.0 48-49 30.689076330108286 35.0 28.5 38.0 7.0 40.0 50-51 30.213276724851255 35.0 28.0 38.0 2.0 40.0 52-53 29.699692659285233 34.0 27.0 38.0 2.0 40.0 54-55 29.338095383083363 34.0 26.5 38.0 2.0 40.0 56-57 28.919206645436994 34.0 26.0 38.0 2.0 40.0 58-59 28.387920041265325 33.0 25.0 37.0 2.0 39.5 60-61 27.817983909388218 33.0 23.5 37.0 2.0 39.0 62-63 27.279234979528532 33.0 22.5 36.0 2.0 39.0 64-65 26.67594718611307 32.0 22.0 35.5 2.0 39.0 66-67 26.092783403601402 31.5 20.5 35.0 2.0 38.0 68-69 25.644817297049457 31.0 20.0 35.0 2.0 38.0 70-71 25.167725463787168 31.0 18.5 35.0 2.0 37.0 72-73 24.576836074205946 30.5 16.0 34.5 2.0 36.5 74-75 23.936615455154403 30.0 11.5 34.0 2.0 36.0 76-77 22.91071895518485 28.5 10.0 32.5 2.0 35.0 78-79 23.541129208472288 30.0 7.5 34.0 2.0 35.0 80-81 23.248242999760002 30.0 6.0 34.0 2.0 35.0 82-83 22.749431348036495 29.5 2.0 34.0 2.0 35.0 84-85 22.200053551791207 29.0 2.0 34.0 2.0 35.0 86-87 21.54154741393206 29.0 2.0 34.0 2.0 35.0 88-89 20.735793551576286 27.5 2.0 33.0 2.0 35.0 90-91 19.921919160078662 26.5 2.0 33.0 2.0 35.0 92-93 18.98414222209486 25.0 2.0 33.0 2.0 34.0 94-95 18.077421740952616 24.0 2.0 32.0 2.0 34.0 96-97 16.95133503361763 19.0 2.0 32.0 2.0 34.0 98-99 15.694821058212051 2.0 2.0 31.5 2.0 34.0 100-101 13.040176380615325 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3870319560493023 1101 2 0.29354711033184344 1101 3 0.46969954164356054 1101 4 0.4857779496168604 1101 5 0.23629824151542067 1101 6 0.3171232089057199 1101 7 0.48071185126788407 1101 8 0.40650232971018596 1101 9 0.3369107317333544 1101 10-11 0.45517376842499147 1101 12-13 0.3871263925834896 1101 14-15 0.7246963108039424 1101 16-17 0.5104970714562995 1101 18-19 0.5527668313184222 1101 20-21 0.6789838485946333 1101 22-23 0.5458762756709987 1101 24-25 0.5369578617354307 1101 26-27 0.5163954198549732 1101 28-29 0.6017524951372053 1101 30-31 0.8625360139267997 1101 32-33 0.8257875125445437 1101 34-35 0.569396360453716 1101 36-37 0.4265130515173965 1101 38-39 0.4941079305936924 1101 40-41 0.5776812966625613 1101 42-43 0.6993356759363607 1101 44-45 0.7080391708679485 1101 46-47 0.9569606858049973 1101 48-49 0.8699614144296604 1101 50-51 0.9689461939120818 1101 52-53 0.9171395541851517 1101 54-55 0.9886959138884706 1101 56-57 0.8458443723658746 1101 58-59 0.9764281847199889 1101 60-61 1.2153497703947096 1101 62-63 1.1580838787527412 1101 64-65 1.1210985740828647 1101 66-67 0.9267836215048924 1101 68-69 1.0855572557459645 1101 70-71 0.9388508944293044 1101 72-73 0.9222192018913162 1101 74-75 1.2262996805029864 1101 76-77 0.9919432609584327 1101 78-79 1.321876966535985 1101 80-81 1.153035564599172 1101 82-83 1.3786776443971398 1101 84-85 1.359924875230952 1101 86-87 1.059154365090663 1101 88-89 1.1063755702003526 1101 90-91 1.3489460620837121 1101 92-93 1.3355632928739958 1101 94-95 1.176694231140658 1101 96-97 1.6651590346345486 1101 98-99 1.320862336103641 1101 100-101 1.4713491646273518 1102 1 0.3124416706966855 1102 2 0.4252752510285944 1102 3 0.5214979768500676 1102 4 0.32579734120575665 1102 5 0.3832231933731123 1102 6 0.3223144988503748 1102 7 0.28079668946931946 1102 8 0.4446054552572605 1102 9 0.4707396278940905 1102 10-11 0.5309221625590368 1102 12-13 0.6374091865882576 1102 14-15 0.5387592526695713 1102 16-17 0.6831596443430854 1102 18-19 0.560927458313131 1102 20-21 0.37993592174401414 1102 22-23 0.6016270937536561 1102 24-25 0.7854058613045041 1102 26-27 0.7115973482407512 1102 28-29 0.706712836644634 1102 30-31 0.794258094752017 1102 32-33 0.7522947372580475 1102 34-35 0.9777340703609312 1102 36-37 0.9338690622366386 1102 38-39 1.0829557470199802 1102 40-41 1.1873818043451294 1102 42-43 1.3214117879227771 1102 44-45 1.382661102216339 1102 46-47 1.2505917608262074 1102 48-49 1.1456653695058137 1102 50-51 1.3298654090913828 1102 52-53 1.4831921538700392 1102 54-55 0.9986711357471023 1102 56-57 0.9987079983236704 1102 58-59 1.360055085163033 1102 60-61 1.5921997145092277 1102 62-63 1.631649372497609 1102 64-65 1.0959265410632995 1102 66-67 1.0509200898117648 1102 68-69 1.2886504835028703 1102 70-71 1.0850318287268301 1102 72-73 1.1204510660424631 1102 74-75 0.9074427069374913 1102 76-77 1.3556937827807083 1102 78-79 1.4108392472023041 1102 80-81 1.3472174146106148 1102 82-83 1.3744761334691695 1102 84-85 1.5968187197481143 1102 86-87 1.6584520933121603 1102 88-89 1.363708014752529 1102 90-91 1.35934884154479 1102 92-93 1.7216781072399314 1102 94-95 1.394927037938487 1102 96-97 1.327927183949786 1102 98-99 1.2672762387955743 1102 100-101 1.4872394944190273 1103 1 -0.017919507041696647 1103 2 0.042953493459158665 1103 3 0.08325059269461121 1103 4 0.14779607239079695 1103 5 0.09116328097348259 1103 6 0.25045916113701594 1103 7 0.17482456863641715 1103 8 0.043518706552148956 1103 9 0.25003752131363655 1103 10-11 0.013732326983550536 1103 12-13 0.019956897507015015 1103 14-15 0.19144940110354014 1103 16-17 0.09588842210927595 1103 18-19 -0.014041255838499467 1103 20-21 0.05864112214725736 1103 22-23 0.14109940577785807 1103 24-25 0.4239044012982518 1103 26-27 -0.11757648809950183 1103 28-29 -0.03424251149733948 1103 30-31 0.4115115883384135 1103 32-33 0.48171451512852315 1103 34-35 0.3692579953444195 1103 36-37 0.19117669772174395 1103 38-39 0.4258725440443456 1103 40-41 0.7746638897207347 1103 42-43 0.4935295791418852 1103 44-45 0.3904934274501173 1103 46-47 0.28165165584480434 1103 48-49 0.6007321561015679 1103 50-51 0.49317536494357483 1103 52-53 0.8631968032947235 1103 54-55 1.195970775959843 1103 56-57 0.724161643125008 1103 58-59 0.7800719447590971 1103 60-61 1.0736237420407733 1103 62-63 0.9800009993617209 1103 64-65 0.8260491642078733 1103 66-67 0.7596521004489567 1103 68-69 1.0485544098768393 1103 70-71 0.6207748241730009 1103 72-73 0.9433318843412053 1103 74-75 1.0335007769482694 1103 76-77 1.062040247479839 1103 78-79 0.9875004388455011 1103 80-81 1.1975939311052137 1103 82-83 1.3865081493206652 1103 84-85 1.813463442868354 1103 86-87 2.0266756654608002 1103 88-89 1.7952897751029333 1103 90-91 1.3608685950818291 1103 92-93 1.202006043764424 1103 94-95 1.0981818524829308 1103 96-97 1.0763129210791327 1103 98-99 1.0371025588845626 1103 100-101 0.9424605376282607 1104 1 0.3363579636712757 1104 2 0.1905966262154415 1104 3 0.3376414746079135 1104 4 0.32579734120575665 1104 5 0.35033829053305254 1104 6 0.17881674100284073 1104 7 0.1088983337144569 1104 8 0.27121233119149224 1104 9 0.23905053970275958 1104 10-11 0.3963930145769723 1104 12-13 0.3616244332250247 1104 14-15 0.7293422123108257 1104 16-17 0.6136529178856875 1104 18-19 0.5863385195986339 1104 20-21 0.625077924733553 1104 22-23 0.6733759726774267 1104 24-25 0.7024462200488983 1104 26-27 0.7788619222317834 1104 28-29 0.7022285317118957 1104 30-31 0.7837947165756347 1104 32-33 0.773968877766265 1104 34-35 0.873100288597108 1104 36-37 1.4308795256148201 1104 38-39 1.0440917709362694 1104 40-41 1.3951545995618702 1104 42-43 0.9394984844848153 1104 44-45 0.72645781372605 1104 46-47 0.9890073064166423 1104 48-49 1.6553813635267396 1104 50-51 1.3709715376414593 1104 52-53 1.2769141269642077 1104 54-55 1.1421688935946328 1104 56-57 0.9860024676809189 1104 58-59 1.0902494050434512 1104 60-61 1.445712420039868 1104 62-63 1.4537719434991025 1104 64-65 1.58695793119783 1104 66-67 1.5456884007235736 1104 68-69 1.5890989139961427 1104 70-71 1.2621618735698767 1104 72-73 1.565144638538726 1104 74-75 1.4814337383276275 1104 76-77 1.5649613463083583 1104 78-79 1.3263848376357856 1104 80-81 1.628233857062039 1104 82-83 1.6323236671014563 1104 84-85 2.3023493624984894 1104 86-87 2.195821301084951 1104 88-89 2.390613844348941 1104 90-91 2.328706091171096 1104 92-93 2.3674180175538346 1104 94-95 2.0062872771014177 1104 96-97 2.6194070045775852 1104 98-99 2.7478442507537153 1104 100-101 2.520124397259085 1105 1 -0.07100188177014033 1105 2 0.03441508397164128 1105 3 0.14173068457822424 1105 4 0.05461478032199096 1105 5 0.21138435366125918 1105 6 0.14910450107078077 1105 7 -0.07944018438185196 1105 8 -0.028098427248913538 1105 9 -0.09285440859839866 1105 10-11 0.046657288698710886 1105 12-13 0.049347189184075546 1105 14-15 0.24261085628356938 1105 16-17 -0.08248220184456301 1105 18-19 -0.11363080674843928 1105 20-21 -0.11564615660198996 1105 22-23 -0.17253355835904927 1105 24-25 0.03682909590988004 1105 26-27 -0.07557557104404822 1105 28-29 -0.5732552078184341 1105 30-31 -0.2794685480624537 1105 32-33 0.1117327226857725 1105 34-35 0.15167657328523632 1105 36-37 0.38696469765051944 1105 38-39 0.27705358681652825 1105 40-41 0.24865357062105176 1105 42-43 0.5195521404866668 1105 44-45 0.4491382331768108 1105 46-47 0.7700237488680592 1105 48-49 0.8815503387291521 1105 50-51 0.9156463396933887 1105 52-53 1.1792837811073262 1105 54-55 0.8614603322118484 1105 56-57 0.907888805773247 1105 58-59 0.6556506690726422 1105 60-61 0.41036561319003084 1105 62-63 0.9650777025918948 1105 64-65 0.8316249503825972 1105 66-67 1.0655713372451352 1105 68-69 1.0167457183279964 1105 70-71 1.1428653989797937 1105 72-73 1.1187365461881384 1105 74-75 1.4976621609006884 1105 76-77 1.0690038590756963 1105 78-79 1.204110271816763 1105 80-81 0.8751899548240054 1105 82-83 1.1699183491141056 1105 84-85 1.5879668528900837 1105 86-87 2.0869275440889545 1105 88-89 1.724282527693834 1105 90-91 1.9525841565334474 1105 92-93 1.204353216028391 1105 94-95 1.3428653793179315 1105 96-97 1.4320836928302647 1105 98-99 1.5263438513095053 1105 100-101 1.5169345662617566 1106 1 0.21329415574900068 1106 2 0.07413081904055119 1106 3 0.09731309269461264 1106 4 0.1028106072745203 1106 5 -0.06610416088697946 1106 6 -0.06948996676995733 1106 7 0.007964103520137655 1106 8 -0.058479839959481694 1106 9 0.22398374224386686 1106 10-11 -0.07611142301644946 1106 12-13 0.24862695564655724 1106 14-15 0.3698469301733027 1106 16-17 -0.09017625812328589 1106 18-19 0.03586790113823923 1106 20-21 -0.1925580057597216 1106 22-23 -0.2552850418965633 1106 24-25 -0.24221115102733393 1106 26-27 -0.00800163344833038 1106 28-29 -0.37646634870664286 1106 30-31 -0.04521079538251627 1106 32-33 0.009294456988989452 1106 34-35 0.30370566976302626 1106 36-37 -0.4252840580922097 1106 38-39 0.03946266032341583 1106 40-41 0.23047784320910836 1106 42-43 0.22308989890932907 1106 44-45 0.1297194448919683 1106 46-47 -0.012007501131940046 1106 48-49 -0.0610774369216891 1106 50-51 0.27658742889706645 1106 52-53 -0.11823488275178562 1106 54-55 0.09155580502961413 1106 56-57 0.08729754428779657 1106 58-59 0.1593415668521203 1106 60-61 -0.11325852540108627 1106 62-63 0.3540744005245138 1106 64-65 0.16164146071950114 1106 66-67 0.4928098039373303 1106 68-69 0.4123262122024194 1106 70-71 0.6840742427776512 1106 72-73 1.0997999075970206 1106 74-75 0.613642492064546 1106 76-77 0.16872629399146533 1106 78-79 0.40812180512457275 1106 80-81 0.5993381171517242 1106 82-83 1.0151782074602025 1106 84-85 1.4823042858916118 1106 86-87 1.699422758484058 1106 88-89 1.493073205335488 1106 90-91 1.3643387985701985 1106 92-93 1.6146877298109352 1106 94-95 1.4525059803899047 1106 96-97 2.087977165265176 1106 98-99 1.5307980530706118 1106 100-101 1.2135906248375612 1107 1 0.09316252391620594 1107 2 0.11961916309200049 1107 3 0.08345155732483533 1107 4 0.1530819095253193 1107 5 0.24707911885578682 1107 6 0.028907340303995 1107 7 0.13007021284489895 1107 8 0.17734712949389575 1107 9 0.18825725510560432 1107 10-11 0.3473336131571827 1107 12-13 0.10935344278481551 1107 14-15 0.02412998885498041 1107 16-17 0.13670527322720005 1107 18-19 -0.05667379902252634 1107 20-21 0.3190024845939732 1107 22-23 0.016090058585746192 1107 24-25 0.18577066167362943 1107 26-27 0.023381505619184395 1107 28-29 0.20244241335123547 1107 30-31 -0.10432956547897732 1107 32-33 -0.09873910410426134 1107 34-35 0.05796611992379752 1107 36-37 0.07011887599139044 1107 38-39 -0.07680542649330135 1107 40-41 0.13387414545990595 1107 42-43 -0.04955630285915902 1107 44-45 -0.13288857359677664 1107 46-47 -0.3328701418071809 1107 48-49 -0.22174363467088654 1107 50-51 -0.5782231619389506 1107 52-53 -0.21513500333056257 1107 54-55 -0.2077835237485246 1107 56-57 -0.5167996221109163 1107 58-59 -0.39787055131508 1107 60-61 -0.16565502861651993 1107 62-63 -0.03477457455587185 1107 64-65 -0.11043199185284536 1107 66-67 -0.02904761165752845 1107 68-69 0.1658079244210704 1107 70-71 0.11919281190948183 1107 72-73 -0.003104031309732136 1107 74-75 0.08528889479766733 1107 76-77 0.014526133219764148 1107 78-79 0.2641633043207179 1107 80-81 0.3189321685986677 1107 82-83 0.2898114470100417 1107 84-85 0.9546691371777882 1107 86-87 1.0618725173264991 1107 88-89 0.7656264609624976 1107 90-91 0.8193553781521921 1107 92-93 0.6985452458881021 1107 94-95 0.7105093967886198 1107 96-97 0.9927350028857553 1107 98-99 1.190562422041669 1107 100-101 1.2209484423616779 1108 1 0.12437486360497729 1108 2 0.15773863409783573 1108 3 0.15004682837382433 1108 4 0.17780549270823087 1108 5 0.36008241849108913 1108 6 0.28711038102054687 1108 7 0.3884469185774222 1108 8 0.31375573696359993 1108 9 0.4742624861063902 1108 10-11 0.44253756429942115 1108 12-13 0.19446114140760073 1108 14-15 0.2986483315644719 1108 16-17 0.370770192367722 1108 18-19 0.5468910701235146 1108 20-21 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100-101 -3.3615716155777733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 29.0 5 96.0 6 311.0 7 598.0 8 1149.0 9 1961.0 10 2764.0 11 3933.0 12 5064.0 13 6838.0 14 8377.0 15 10569.0 16 12662.0 17 14173.0 18 12176.0 19 9591.0 20 9481.0 21 9914.0 22 10883.0 23 11632.0 24 13154.0 25 15089.0 26 17002.0 27 19687.0 28 22566.0 29 25657.0 30 29381.0 31 32759.0 32 37024.0 33 41860.0 34 47763.0 35 50539.0 36 41337.0 37 26205.0 38 5994.0 39 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81438774431739 22.285078613282124 16.1541920216968 17.746341620703674 2 18.81094481024267 25.476274064468058 34.955363859772305 20.75741726551697 3 22.240790395179676 28.50781743963403 30.24540437719303 19.00598778799326 4 15.07259043805007 20.173264223463207 34.48090583123484 30.27323950725188 5 12.60520306273228 40.752787355035146 31.77181748981328 14.870192092419291 6 28.821609849231113 38.21950144894311 16.012164674444513 16.94672402738126 7 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34.84079136845273 18.75488067860239 30-31 19.888685902602404 26.971235649168058 34.12480074507908 19.015277703150467 32-33 19.591385658107672 27.374814504349352 33.932292856100155 19.101506981442824 34-35 20.443986330615697 26.604522789471424 33.67339628318838 19.278094596724507 36-37 21.034358312594645 26.600446146723343 33.30249768732655 19.06269785335546 38-39 20.69814716970994 26.523413362945185 33.93007782066322 18.848361646681653 40-41 20.172852596191905 26.854301255335038 33.7464649485884 19.226381199884656 42-43 20.134512481809022 27.616702115750584 32.906750251875074 19.34203515056532 44-45 20.396826605078182 27.546189123089505 31.897204070356306 20.15978020147601 46-47 20.66635323616988 28.23328184118029 30.84935187050843 20.2510130521414 48-49 21.38470383425034 27.924762150389732 30.304243323016966 20.38629069234296 50-51 21.233643420293802 28.61931303260749 29.75115431871017 20.39588922838854 52-53 21.35280426767729 28.69792678787792 29.59380230380783 20.355466640636962 54-55 20.4483887289384 28.598281489972372 29.806880308776577 21.146449472312646 56-57 21.20455705725878 28.969109098888502 28.797055109224278 21.02927873462844 58-59 21.709975904477826 28.09591630165086 29.04450953519827 21.149598258673045 60-61 21.312961400453943 28.357418862489453 29.563934584813488 20.765685152243115 62-63 21.988440900903967 28.112363112985584 29.76553102802181 20.133664958088637 64-65 21.84277536792004 28.314191310360513 29.604408143816407 20.23862517790303 66-67 21.248805580257393 28.714343156456223 29.542444326830854 20.494406936455526 68-69 21.450402939456545 28.53683589317873 29.461908587056683 20.55085258030804 70-71 21.257483153110798 28.96250027984866 29.00020149103365 20.779815076006898 72-73 21.502566545712455 28.449254752497595 28.77181922061547 21.276359481174488 74-75 21.14856599400714 29.423316992966537 28.491611606735074 20.93650540629125 76-77 21.230229981919184 28.79166580844721 29.166625621388203 20.811478588245407 78-79 20.954828055003773 28.572746373294116 29.515627210996453 20.956798360705655 80-81 20.698358447439507 29.588071336958937 28.842440331606518 20.87112988399504 82-83 20.886211433582062 29.211103079939416 28.706214066850993 21.196471419627528 84-85 20.710001397393683 28.699062313009016 28.493125898004596 22.097810391592706 86-87 21.335652354591726 28.591490135352863 28.30892658990133 21.763930920154078 88-89 21.339661823544063 28.295088253053603 28.646362461947533 21.7188874614548 90-91 21.120202692593352 28.43403307551786 28.672347387009 21.77341684487978 92-93 20.808031767330995 28.91468486617752 28.43706415542559 21.840219211065897 94-95 20.617471619792557 28.769867040978752 28.086843031677084 22.525818307551603 96-97 20.759869514460117 28.449144774978745 27.868303141454604 22.922682569106538 98-99 21.074828152215204 28.26681026140933 27.64348125264312 23.01488033373234 100-101 21.343108520164908 28.394307285781746 27.41744569780372 22.84513849624963 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3977.0 1 2770.5 2 2173.0 3 2654.0 4 2136.0 5 1861.5 6 2741.0 7 4902.5 8 6458.0 9 6585.5 10 5297.5 11 3529.5 12 2825.5 13 2602.0 14 2518.5 15 2367.5 16 2183.5 17 2063.5 18 1990.0 19 1961.0 20 1982.5 21 1979.0 22 1989.0 23 2141.0 24 2370.0 25 2590.0 26 2891.0 27 3249.0 28 3696.0 29 4423.0 30 5247.0 31 6165.5 32 7579.5 33 8704.5 34 9542.5 35 10350.0 36 11073.0 37 11900.0 38 12475.0 39 12660.5 40 12782.0 41 13452.0 42 14091.0 43 14707.5 44 15401.5 45 16051.5 46 16584.0 47 18513.0 48 25521.5 49 26455.5 50 21029.0 51 17685.5 52 14993.5 53 14697.5 54 14690.5 55 14079.5 56 13307.5 57 12358.0 58 11792.5 59 11255.0 60 9938.5 61 8465.5 62 7346.0 63 5978.5 64 4727.5 65 3902.5 66 3021.0 67 2459.5 68 1880.5 69 1284.5 70 1001.5 71 819.0 72 714.5 73 617.5 74 519.0 75 455.5 76 343.5 77 237.5 78 168.0 79 126.0 80 85.0 81 60.5 82 50.5 83 34.5 84 15.0 85 9.5 86 7.0 87 3.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017731195082548563 2 0.00823873710906297 3 0.006268604322113128 4 0.0 5 0.0023283387482134475 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.850663934749201E-4 14-15 8.955149031590184E-5 16-17 8.955149031590184E-5 18-19 0.0013432723547385276 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0012537208644226258 28-29 0.0025074417288452515 30-31 0.0014328238450544294 32-33 0.006537258793060835 34-35 0.0017910298063180368 36-37 2.6865447094770554E-4 38-39 5.373089418954111E-4 40-41 1.7910298063180367E-4 42-43 0.004567126006110994 44-45 0.0015223753353703312 46-47 8.955149031590184E-5 48-49 3.5820596126360734E-4 50-51 0.0 52-53 2.6865447094770554E-4 54-55 9.850663934749201E-4 56-57 0.014865547392439706 58-59 0.025611726230347926 60-61 0.02131325469518464 62-63 0.013701378018332981 64-65 0.01764164359223266 66-67 0.002238787257897546 68-69 6.268604322113129E-4 70-71 8.955149031590184E-5 72-73 0.0 74-75 0.0012537208644226258 76-77 0.0020596842772657425 78-79 0.008776046050958379 80-81 0.015402856334335116 82-83 0.017283437630969054 84-85 0.02794006497856137 86-87 0.010119318405696909 88-89 0.040656376603419433 90-91 0.045044399628898626 92-93 0.027671410507613667 94-95 0.02838782243014088 96-97 0.04808915029963928 98-99 0.05122345246069585 100-101 0.058477123176283896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 558338.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.3047527531249 #Duplication Level Percentage of deduplicated Percentage of total 1 74.73511208107915 33.111206627292894 2 12.594046727670296 11.159522528614687 3 4.7245373900490835 6.279583828170561 4 2.366558675619425 4.193991879963253 5 1.3597592713020576 3.0121899159403482 6 0.8639831353455986 2.2967135516613837 7 0.5912827874513579 1.8337646393647637 8 0.3818938724819066 1.3535770878595426 9 0.28738844502161653 1.1459406600708977 >10 1.879154604935048 15.740079295748902 >50 0.1452707640656606 4.499217120347792 >100 0.06046185429621659 5.193354308319412 >500 0.004869411084259055 1.5720112878438375 >1k 0.004057842570215879 3.4136951433644542 >5k 0.0016231370280863514 5.195152125437257 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8767 1.5701958311990227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8281 1.4831517826119662 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6104 1.0932445937765296 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5745 1.0289466237297122 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3062 0.5484133266945829 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2395 0.4289516386131698 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2271 0.4067428690148261 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2057 0.36841483115962015 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2000 0.3582059612636074 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1864 0.33384795589768207 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1709 0.30608699389975247 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1407 0.25199789374894777 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1208 0.21635640060321884 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1015 0.18178952534128073 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 893 0.1599389617042007 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 885 0.15850613785914625 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 847 0.1517002245951377 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 831 0.14883457690502885 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 786 0.1407749427765977 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 737 0.1319988967256393 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 720 0.12895414605489866 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 647 0.11587962846877697 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 636 0.11390949568182714 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 617 0.11050653904982287 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 581 0.10405883174707793 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 564 0.10101408107633728 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.955149031590184E-5 0.0 22-23 0.0 0.0 0.0 1.7910298063180367E-4 0.0 24-25 0.0 0.0 0.0 0.0012537208644226258 0.0 26-27 0.0 0.0 0.0 0.0024178902385293497 0.0 28-29 0.0 0.0 0.0 0.002776096199792957 0.0 30-31 0.0 0.0 0.0 0.005552192399585914 0.0 32-33 0.0 0.0 0.0 0.008865597541274282 0.0 34-35 0.0 0.0 0.0 0.01513420186338741 0.0 36-37 0.0 0.0 0.0 0.025969932191611533 0.0 38-39 0.0 0.0 0.0 0.041910097467842064 0.0 40-41 0.0 0.0 0.0 0.05973084404070653 0.0 42-43 0.0 0.0 0.0 0.07862620849736182 0.0 44-45 0.0 0.0 0.0 0.10110363256665317 0.0 46-47 0.0 0.0 0.0 0.13396902951258916 0.0 48-49 0.0 0.0 0.0 0.16504339665220708 0.0 50-51 0.0 0.0 0.0 0.19755058763687944 0.0 52-53 0.0 0.0 0.0 0.23068463905376313 0.0 54-55 0.0 0.0 0.0 0.2638186904706468 0.0 56-57 0.0 0.0 0.0 0.3124451497121815 0.0 58-59 0.0 0.0 0.0 0.36760886774677703 0.0 60-61 0.0 0.0 0.0 0.425996439432745 0.0 62-63 0.0 0.0 0.0 0.4840258051574494 0.0 64-65 0.0 0.0 0.0 0.5443835096303673 0.0 66-67 0.0 0.0 0.0 0.6201440704376202 0.0 68-69 0.0 0.0 0.0 0.7068299130634133 0.0 70-71 0.0 0.0 0.0 0.8068589277462755 0.0 72-73 0.0 0.0 0.0 0.9041118462293449 0.0 74-75 0.0 0.0 0.0 1.013633318885693 0.0 76-77 0.0 0.0 0.0 1.1246771668774111 0.0 78-79 0.0 0.0 0.0 1.2551536882676801 0.0 80-81 0.0 0.0 0.0 1.4090031486303995 0.0 82-83 0.0 0.0 0.0 1.5639272268769098 0.0 84-85 0.0 0.0 0.0 1.7394481478960773 0.0 86-87 0.0 0.0 0.0 1.9241928724177828 0.0 88-89 0.0 0.0 0.0 2.1127883110230723 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 165 0.0 43.173023 2 GGTAAGG 195 0.0 36.531025 3 AAGGTAA 240 0.0 29.686771 1 GTAAGGT 260 0.0 29.224815 4 AGGTGTG 270 0.0 28.144936 7 TAAGGTG 270 0.0 28.142416 5 GCTTATG 85 6.4039376E-4 27.94049 1 GAGTACG 70 0.008388941 27.14219 1 GTACTAG 90 8.943042E-4 26.38824 1 AAGGTGT 275 0.0 25.903812 6 GGTAGGG 165 2.4304609E-6 23.025612 2 GTGTGCA 460 0.0 22.7148 9 GAGTACT 6740 0.0 21.59999 12-13 GAGTGCA 220 5.6330464E-8 21.588446 9 GCTAAAC 110 0.0028789327 21.586514 3 AGTACTT 6765 0.0 21.555273 12-13 GGATTAT 155 3.9025297E-5 21.451088 1 GTATATG 210 8.593415E-7 20.356646 1 TATATGG 140 4.854102E-4 20.352997 2 GTACTTT 7110 0.0 20.307106 14-15 >>END_MODULE