FastQCFastQC Report
Thu 26 May 2016
SRR936387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682294
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT178131.0588517821498502No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT119360.7095073750485944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94510.5617924096501563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77080.4581838846242096No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT71190.42317216847946915No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC69020.4102731151629858No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT50990.3030980316163524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48500.2882968137555029No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT43260.2571488693415063No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC40990.24365538960490854No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37550.22320712075297183No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33950.20180776962885205No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC32100.19081088085673492No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30480.18118117285088098No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA27810.16530998743382547No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.1232245968897232No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20190.12001469422110522No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC19540.11615092249036138No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19220.11424875794599518No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT18740.11139551112944587No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18650.11086052735134286No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC18600.11056331414128565No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1056.89944E-527.1476332
GTAAGCG1900.026.25813594-95
GTACATG107400.024.7273481
TACCTGG13000.024.4850772
TACATGG108500.024.0387922
ACATGGG110000.022.8451613
GAGTACT80900.021.93501712-13
GTACCTG15600.021.3178521
AGTACTT83550.020.95496412-13
GTATCGA1150.003720190820.6558071
CATGGGG35950.020.6149754
GTACTTT88650.020.07099514-15
CATGGGA71100.020.0451264
ATTCGCG1900.020.00679280-81
GTGTAGC8950.019.1095071
AATTCGC1809.640644E-1118.4804278-79
GTGCCGT1559.6137595E-418.3908796
GTACACG3105.5752025E-918.3903311
TACTTTT97500.018.15170914-15
TCGCGCT2101.8189894E-1218.09142760-61
Produced by FastQC (version 0.11.5)