FastQCFastQC Report
Thu 26 May 2016
SRR936387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682294
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT212541.2633939133112286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105640.6279520702088933No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT98950.5881849427032374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93170.5538270956206227No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT63380.3767474650685314No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC62550.37181372578158156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58160.3457184059385577No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT53800.3198014140215682No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC49640.2950732749448075No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC46060.2737928091047106No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT41250.2452008982972061No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG40120.2384838797499129No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA38190.22701144984170427No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA33630.19990560508448582No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30500.1813000581349039No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.1553236235759029No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC23450.13939299551683595No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21360.12696948333644417No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21170.12584007313822673No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT19040.11317879038978919No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA18810.11181160962352597No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT17240.10247911482772927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15000.031.358252
GTACCTG16700.028.7376691
GTACACG3150.028.6608921
TATAACG1056.8953435E-527.152
ATAACGC1151.2805845E-424.7869223
GTAAGCG1950.024.3559794-95
GTACATG104200.024.2599871
TACATGG106750.023.500332
ACATGGG106800.022.419633
GAGTACT102650.021.88771212-13
ATGGGAG27300.020.5322655
GTACTTT110300.020.11216714-15
AGTACTT106100.020.0567612-13
CATGGGA70800.019.9269454
ACCTGGG24800.018.198733
ACTTTTT123150.018.14831416-17
GTGTAGC7900.018.0443331
TACTTTT119950.017.60309414-15
TAAGGTG8100.017.5935615
AGAGTAC185600.017.41521810-11
Produced by FastQC (version 0.11.5)