FastQCFastQC Report
Thu 26 May 2016
SRR936386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706613
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT176871.03638024555069No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT116280.6813495502495293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98550.5774595646464664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78930.4624950120501836No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT71570.41936865592843836No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC68560.401731382568866No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT49340.2891106536748519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47810.2801455280136739No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT42840.2510235185129845No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC40140.23520270852267033No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC38260.22418673712200715No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33620.1969983821756895No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC32580.19090444054979072No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31670.1855722416271293No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA28000.16406765915881338No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20780.12176164133286223No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20520.12023815592638752No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC19540.11449578785582906No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19290.11303089804191109No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18530.10857763300760044No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC18260.10699555200856903No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT17450.10224930901147476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1452.3914254E-829.4954972
CTATCGG908.923734E-426.4018381
GTACATG107400.026.3723371
TACATGG108500.025.4464742
ACATGGG108500.024.2639373
GAGTACT83900.023.38799312-13
TACCTGG16050.022.501932
GTACCTG17100.022.2331281
GTACTTT93950.020.96076414-15
GGGTCGA1150.003714066520.6617036
AGTACTT87850.020.65981512-13
CATGGGG34950.020.3950734
CATGGGA73500.020.3659384
ATAACGC2151.0749591E-619.8923133
GTATAGG4800.019.801381
ATGGGAT27300.018.9733965
ATGGGAG28150.018.738115
ACTTTTT108100.018.65600216-17
TAACGCA2051.5842235E-518.5445944
GTACACG3208.229108E-917.8212411
Produced by FastQC (version 0.11.5)