##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936386_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1706613 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.312170363169624 31.0 31.0 34.0 30.0 34.0 2 31.334948813820123 31.0 31.0 34.0 28.0 34.0 3 31.370620638656803 31.0 31.0 34.0 28.0 34.0 4 35.01042708569547 37.0 35.0 37.0 32.0 37.0 5 34.95201782712308 37.0 35.0 37.0 32.0 37.0 6 35.09125267415636 37.0 35.0 37.0 32.0 37.0 7 34.94033093618764 37.0 35.0 37.0 32.0 37.0 8 34.92991205387513 37.0 35.0 37.0 32.0 37.0 9 36.416373835192864 39.0 37.0 39.0 32.0 39.0 10-11 36.27581619265762 39.0 35.0 39.0 32.0 39.0 12-13 36.1832407229993 39.0 35.0 39.0 31.5 39.0 14-15 37.174782742191695 39.0 36.0 41.0 31.0 41.0 16-17 37.13977832115424 39.0 36.0 41.0 31.0 41.0 18-19 37.15613176508089 39.0 36.5 41.0 31.0 41.0 20-21 37.06329349418995 39.0 36.0 41.0 31.0 41.0 22-23 36.94827942831796 39.0 36.0 41.0 31.0 41.0 24-25 36.78149615642211 39.0 36.0 41.0 30.0 41.0 26-27 36.56311243380895 39.0 36.0 40.5 30.0 41.0 28-29 36.684886673194214 39.0 36.0 41.0 30.0 41.0 30-31 36.600062521497264 39.0 36.0 41.0 30.0 41.0 32-33 36.3985042303088 39.0 35.0 40.5 30.0 41.0 34-35 36.17802278548212 39.0 35.0 40.0 30.0 41.0 36-37 35.907944566225616 38.0 35.0 40.0 28.5 41.0 38-39 35.705763110910326 38.0 35.0 40.0 28.0 41.0 40-41 35.575552278108745 38.0 35.0 40.0 27.0 41.0 42-43 35.273588681206576 38.0 34.5 40.0 26.5 41.0 44-45 34.9437792868096 38.0 34.0 40.0 25.5 41.0 46-47 34.51780925142373 38.0 33.5 40.0 24.0 41.0 48-49 34.17258863022841 38.0 33.0 40.0 23.0 41.0 50-51 33.42024934768457 37.5 32.0 39.5 21.5 40.5 52-53 33.611232892284306 37.5 33.0 39.5 22.0 40.5 54-55 33.96628819773434 38.0 33.0 40.0 21.5 41.0 56-57 33.843831319695795 38.0 33.0 40.0 20.5 41.0 58-59 33.60452193906879 38.0 33.0 40.0 20.0 41.0 60-61 33.231885905005996 37.0 32.5 40.0 18.5 41.0 62-63 32.94603111543156 37.0 32.0 40.0 18.0 41.0 64-65 32.6513855220838 36.0 32.0 39.0 15.5 41.0 66-67 32.23255887538651 36.0 31.0 39.0 13.0 41.0 68-69 31.834359928114928 35.0 31.0 39.0 9.0 40.0 70-71 31.396473893026716 35.0 31.0 38.0 7.5 40.0 72-73 30.974286789096297 35.0 30.5 37.0 7.0 39.5 74-75 30.56447507431386 35.0 30.0 37.0 2.0 39.0 76-77 30.154379463885483 34.5 30.0 36.0 2.0 39.0 78-79 29.678925743563422 34.0 29.0 36.0 2.0 38.5 80-81 29.2232090696602 34.0 29.0 35.5 2.0 37.0 82-83 28.79850118333799 34.0 28.5 35.0 2.0 37.0 84-85 28.404011923031174 34.0 27.5 35.0 2.0 36.5 86-87 28.10353050164273 34.0 27.0 35.0 2.0 36.0 88-89 27.69302735886812 34.0 26.5 35.0 2.0 36.0 90-91 27.301047747790506 33.5 26.0 35.0 2.0 35.0 92-93 26.91385275982311 33.0 25.0 35.0 2.0 35.0 94-95 26.48074109361642 33.0 25.0 35.0 2.0 35.0 96-97 25.903337487760844 33.0 23.5 35.0 2.0 35.0 98-99 25.26973807184171 32.5 19.0 35.0 2.0 35.0 100-101 23.205076956521484 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22387582996993416 1101 2 0.23670629057870585 1101 3 0.17544280321992645 1101 4 0.2587245718065745 1101 5 0.18449655869441983 1101 6 0.21170976977291645 1101 7 0.22899237126939198 1101 8 0.08802082780276521 1101 9 0.5164997841809154 1101 10-11 0.40530431462133976 1101 12-13 0.5579347178678873 1101 14-15 0.5369283412128993 1101 16-17 0.7283533747309718 1101 18-19 0.5702999373902529 1101 20-21 0.46977732907939895 1101 22-23 0.49371643856094494 1101 24-25 0.5309523915251404 1101 26-27 0.4935433261951232 1101 28-29 0.5509081430972387 1101 30-31 0.3578171469791087 1101 32-33 0.31774775197690275 1101 34-35 0.562789529744876 1101 36-37 0.6063764879602545 1101 38-39 0.5082188908318486 1101 40-41 0.4235052004341213 1101 42-43 0.37247520146926405 1101 44-45 0.5588848350245783 1101 46-47 0.6321828117090078 1101 48-49 0.627565546546414 1101 50-51 0.5369104539742153 1101 52-53 0.7308191956970376 1101 54-55 0.7119309984638562 1101 56-57 0.565326817850881 1101 58-59 0.808275226586531 1101 60-61 0.6315128168509787 1101 62-63 0.5397602307590716 1101 64-65 0.7052221301386083 1101 66-67 0.6680948276507479 1101 68-69 0.5346397086509533 1101 70-71 0.48823883925592426 1101 72-73 0.5478383259834381 1101 74-75 0.5041696459643603 1101 76-77 0.3936680397343686 1101 78-79 0.49747691930092586 1101 80-81 0.44057158222501513 1101 82-83 0.3901967989423163 1101 84-85 0.5822101965308413 1101 86-87 0.6227203661200633 1101 88-89 0.38642669959367026 1101 90-91 0.48001648393710283 1101 92-93 0.3503715597517747 1101 94-95 0.31491764945629797 1101 96-97 0.4508902588885775 1101 98-99 0.4628769321385491 1101 100-101 0.7993384121860494 1102 1 0.10696644485666695 1102 2 0.3120353314853972 1102 3 0.25312624691060037 1102 4 0.25706639069415615 1102 5 0.2757924337220601 1102 6 0.3202103317152023 1102 7 0.27472014295381797 1102 8 0.24672382437695006 1102 9 0.6413903295583196 1102 10-11 0.6004497764295778 1102 12-13 0.5442816641967596 1102 14-15 0.5138294664515612 1102 16-17 0.4686516893554682 1102 18-19 0.5490719467965803 1102 20-21 0.23461068723748468 1102 22-23 -0.01768828175428183 1102 24-25 0.04242509783018278 1102 26-27 0.2710339266383599 1102 28-29 0.3279679775386626 1102 30-31 0.22914223623539698 1102 32-33 0.1775008358890986 1102 34-35 0.11421796898966363 1102 36-37 0.28064714780237665 1102 38-39 0.03311332918294596 1102 40-41 -0.10527572331775303 1102 42-43 0.1500036032512284 1102 44-45 0.27288808706811096 1102 46-47 0.331299670880405 1102 48-49 0.3190527414839721 1102 50-51 0.4334137737216395 1102 52-53 0.2394144866657939 1102 54-55 0.1381735341099528 1102 56-57 0.3074692171864797 1102 58-59 0.27185617561452347 1102 60-61 -0.08419149543547633 1102 62-63 0.16144386853423498 1102 64-65 0.43604048962821196 1102 66-67 0.30638707600667203 1102 68-69 0.2126978369543373 1102 70-71 0.39046454822848986 1102 72-73 0.05601921272385724 1102 74-75 -0.03710038874516641 1102 76-77 0.036695611015279184 1102 78-79 -0.011524877108957554 1102 80-81 0.1254116656362072 1102 82-83 0.13161928024475955 1102 84-85 0.10925282290014948 1102 86-87 0.015725087338008592 1102 88-89 -0.35143088978674797 1102 90-91 -0.4030811834382 1102 92-93 -0.2301306225702895 1102 94-95 -0.04325595911591762 1102 96-97 -0.2743010610213297 1102 98-99 -0.27150349827764586 1102 100-101 0.15693371669557976 1103 1 0.12065352321681289 1103 2 0.330644753202332 1103 3 0.18369884116142288 1103 4 0.10066718148262055 1103 5 0.15824271631711895 1103 6 -0.0017355138226591293 1103 7 0.04632053422257343 1103 8 -0.08111710815416018 1103 9 0.09982318093481979 1103 10-11 0.18603479596044536 1103 12-13 0.2490515278172296 1103 14-15 0.3102668471656145 1103 16-17 0.3350170968112849 1103 18-19 0.2239386288071188 1103 20-21 0.1866132254904116 1103 22-23 0.25775178703258206 1103 24-25 0.1597958184638557 1103 26-27 0.2314513487374441 1103 28-29 0.2851869349659282 1103 30-31 0.24008519502624637 1103 32-33 0.24229006753019888 1103 34-35 0.08838028038487522 1103 36-37 0.3195450868017886 1103 38-39 0.23391065869768113 1103 40-41 0.21539963869269485 1103 42-43 0.2666720431484819 1103 44-45 0.36622085767214685 1103 46-47 0.43496214295202407 1103 48-49 0.6670141867465205 1103 50-51 0.06431456038463068 1103 52-53 0.41680327253843075 1103 54-55 0.4874372182027784 1103 56-57 0.3415491710680101 1103 58-59 0.3885257011631609 1103 60-61 0.5532453196542093 1103 62-63 0.4008639244235681 1103 64-65 0.4892146085678277 1103 66-67 0.26485751806220037 1103 68-69 0.21588571652520372 1103 70-71 0.29189160982618034 1103 72-73 0.20478547849565487 1103 74-75 -0.004601414685723171 1103 76-77 0.13028486675783313 1103 78-79 0.31314595339263107 1103 80-81 0.4403545169475649 1103 82-83 0.35117137305492463 1103 84-85 0.22843535998057263 1103 86-87 0.13929094186506674 1103 88-89 -0.08496630807736949 1103 90-91 0.02939876458081514 1103 92-93 0.07805106335945311 1103 94-95 -0.03668247571661354 1103 96-97 0.008176878542627719 1103 98-99 0.14184564394919974 1103 100-101 0.4384435266414535 1104 1 0.3917282263170492 1104 2 0.42825652110466805 1104 3 0.47211655912552786 1104 4 0.43765523078178603 1104 5 0.2658546535270929 1104 6 0.3508541003090997 1104 7 0.47736489094356216 1104 8 0.4107756088491925 1104 9 0.5945269422787689 1104 10-11 0.4923201137361062 1104 12-13 0.6224532166475214 1104 14-15 0.5341630352443687 1104 16-17 0.43724263232732596 1104 18-19 0.4936220530783615 1104 20-21 0.5034237006348476 1104 22-23 0.4609475628337947 1104 24-25 0.4901307492909339 1104 26-27 0.4342054465870504 1104 28-29 0.4391593356703609 1104 30-31 0.2969362825053494 1104 32-33 0.3212994942034584 1104 34-35 0.39863035314684936 1104 36-37 0.4678099387772008 1104 38-39 0.24601830503390687 1104 40-41 0.08539878140479829 1104 42-43 0.34669207519868905 1104 44-45 0.20771262461775564 1104 46-47 0.32559948410182216 1104 48-49 0.1663654428614123 1104 50-51 -0.01040909863837669 1104 52-53 0.1330356023970154 1104 54-55 0.3286306812078479 1104 56-57 0.32931521585606305 1104 58-59 0.35157726447736337 1104 60-61 0.3782105108092608 1104 62-63 0.17364433483066932 1104 64-65 0.09804166403650783 1104 66-67 0.08135611778050844 1104 68-69 0.3090457684139629 1104 70-71 0.39253425326132785 1104 72-73 0.1162049295787817 1104 74-75 0.052572835395643835 1104 76-77 0.1295346726587141 1104 78-79 0.2298758864731134 1104 80-81 0.51389785983827 1104 82-83 0.22716427503359782 1104 84-85 0.24452402021157837 1104 86-87 0.21186237826841747 1104 88-89 0.15388821016579968 1104 90-91 0.05576184562515607 1104 92-93 -0.14168055984128358 1104 94-95 0.030079961743698647 1104 96-97 0.1420761114715141 1104 98-99 0.11808573508508147 1104 100-101 0.13635435096273696 1105 1 0.17933486590929704 1105 2 0.1789898979409159 1105 3 0.1451643658357753 1105 4 0.23340236661722713 1105 5 0.3122397697584347 1105 6 0.196710821803336 1105 7 0.3079063418993755 1105 8 0.11822412015700934 1105 9 0.288291007909379 1105 10-11 0.24547835051060218 1105 12-13 0.0963071099178805 1105 14-15 0.2888245008009065 1105 16-17 0.24373389246353128 1105 18-19 0.1780595801674778 1105 20-21 -0.14002876334564718 1105 22-23 -0.1510354325503016 1105 24-25 -0.08063964777738164 1105 26-27 -0.17990058722444502 1105 28-29 -0.039177388645690314 1105 30-31 0.01982899651537906 1105 32-33 0.03605157836182826 1105 34-35 -0.0130100968678164 1105 36-37 -0.056836012164986016 1105 38-39 -0.13758334080854695 1105 40-41 -0.2930302129136422 1105 42-43 -0.09656254137442488 1105 44-45 -0.08551719422520421 1105 46-47 -0.01633716517971351 1105 48-49 0.18045846320891457 1105 50-51 0.17167972533461295 1105 52-53 0.29457557884985874 1105 54-55 0.28565578730632524 1105 56-57 0.3873616784651972 1105 58-59 0.023159119165875097 1105 60-61 0.30828732498500955 1105 62-63 0.12688671821319986 1105 64-65 0.13344777229463745 1105 66-67 -0.1466818580192637 1105 68-69 0.0733176904892332 1105 70-71 0.24400079316028922 1105 72-73 0.05049762509739608 1105 74-75 0.10723272437235565 1105 76-77 0.3010234022506886 1105 78-79 0.13954694778924548 1105 80-81 0.19456428814744342 1105 82-83 0.4119254018125247 1105 84-85 0.10914217524976522 1105 86-87 0.26912709777883137 1105 88-89 0.2939047989921768 1105 90-91 0.1958046217096694 1105 92-93 0.18746548435960264 1105 94-95 0.4154074280623412 1105 96-97 0.3288881281095115 1105 98-99 0.02585180698033085 1105 100-101 0.2328609440057967 1106 1 0.03450076375846223 1106 2 0.03113119907872175 1106 3 -0.11511877259605541 1106 4 0.07441461212010836 1106 5 0.1591197655738057 1106 6 0.17209395010050343 1106 7 0.32675354064659956 1106 8 0.32544577389381146 1106 9 0.34018646180594203 1106 10-11 0.2789044759210313 1106 12-13 0.22162599754629042 1106 14-15 0.23854868547838493 1106 16-17 -0.0035676180367403276 1106 18-19 0.14434031243482082 1106 20-21 0.18534423029131375 1106 22-23 0.04058647270043281 1106 24-25 -0.003144096675264052 1106 26-27 0.18504171604600117 1106 28-29 -0.2354813979390613 1106 30-31 0.0014735467380617706 1106 32-33 -0.1217170201018476 1106 34-35 -0.06447945913615172 1106 36-37 -0.019536376031972225 1106 38-39 -0.047311034475910674 1106 40-41 -8.035517828943739E-4 1106 42-43 0.04747767905988809 1106 44-45 -0.2814005606323988 1106 46-47 -0.24674103683786797 1106 48-49 -0.06432954258419699 1106 50-51 0.08690111317280014 1106 52-53 0.012173816944624605 1106 54-55 0.1005086222323257 1106 56-57 0.133336965150761 1106 58-59 -0.02528564639110442 1106 60-61 -0.05410115288710671 1106 62-63 -0.06961998428440097 1106 64-65 -0.1692181337276395 1106 66-67 -0.1339334362473963 1106 68-69 -0.15534421833587686 1106 70-71 0.009387495832243076 1106 72-73 -0.04519890394494652 1106 74-75 -0.032428069640129564 1106 76-77 -0.318328567959945 1106 78-79 -0.2236520304626488 1106 80-81 -0.25102648081172774 1106 82-83 -0.24292784721787442 1106 84-85 -0.3024143886863193 1106 86-87 -0.0673444385375781 1106 88-89 0.029206544878107366 1106 90-91 0.07270200990965492 1106 92-93 -0.044776520428751354 1106 94-95 0.008486108987273866 1106 96-97 -0.03517856231367489 1106 98-99 0.32420476123377995 1106 100-101 0.6764356784068646 1107 1 0.26778473256779023 1107 2 0.343378008578604 1107 3 0.26781811727903104 1107 4 0.15744879602797113 1107 5 0.21040977251723092 1107 6 0.24511736524405592 1107 7 0.38167331612001476 1107 8 0.5366150304554722 1107 9 0.5935153383919669 1107 10-11 0.49707742682689826 1107 12-13 0.42302956544516235 1107 14-15 0.5606828011820255 1107 16-17 0.5765026956596415 1107 18-19 0.6378400009340126 1107 20-21 0.8417070263323936 1107 22-23 0.6712755137145976 1107 24-25 0.5900349274200778 1107 26-27 0.7268191095130376 1107 28-29 0.43729115604159574 1107 30-31 0.5048370174413108 1107 32-33 0.5094548010370303 1107 34-35 0.33976248689210564 1107 36-37 0.3942567751323267 1107 38-39 0.6174616683363325 1107 40-41 0.3206191551191395 1107 42-43 0.35171525854178753 1107 44-45 0.34476971991512784 1107 46-47 0.49562780840742704 1107 48-49 0.3954754757460748 1107 50-51 0.343317322530865 1107 52-53 0.40116959738483615 1107 54-55 0.5764935398122262 1107 56-57 0.5516527205725055 1107 58-59 0.5402380716431665 1107 60-61 0.3870201208652162 1107 62-63 0.5468767048279588 1107 64-65 0.3431081346156972 1107 66-67 0.4291925250846873 1107 68-69 0.5249520180086726 1107 70-71 0.48967868912326296 1107 72-73 0.4763781388453623 1107 74-75 0.20248505146265217 1107 76-77 0.3986615716403854 1107 78-79 0.4935252248739168 1107 80-81 0.5239944447853979 1107 82-83 0.5433842907354709 1107 84-85 0.5771376590086668 1107 86-87 0.6092322383238447 1107 88-89 0.48566623519771923 1107 90-91 0.4233281480561111 1107 92-93 0.3850357057780265 1107 94-95 0.36724355594757796 1107 96-97 0.8288702470644367 1107 98-99 1.007291282197201 1107 100-101 1.1769673515505765 1108 1 0.14331396258716467 1108 2 0.09606995246250705 1108 3 0.060696432741266904 1108 4 0.04528682407839568 1108 5 0.3217624693542547 1108 6 0.1267490443453454 1108 7 0.384484014057044 1108 8 0.2282916978815308 1108 9 0.3205462392292304 1108 10-11 0.18534287414760087 1108 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2316 80-81 -2.1220641970861784 2316 82-83 -1.9466007044125213 2316 84-85 -2.236593716277273 2316 86-87 -2.061873700525961 2316 88-89 -2.1657306795120164 2316 90-91 -2.4048903331397504 2316 92-93 -2.790213265430271 2316 94-95 -2.7779886916788534 2316 96-97 -3.2504344586392335 2316 98-99 -3.510408870063543 2316 100-101 -4.3350256816375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4611.0 3 1755.0 4 1748.0 5 1883.0 6 2106.0 7 2588.0 8 3085.0 9 3833.0 10 4867.0 11 6536.0 12 8350.0 13 10128.0 14 11576.0 15 13663.0 16 17076.0 17 21058.0 18 18487.0 19 12656.0 20 12511.0 21 13017.0 22 14285.0 23 16233.0 24 18162.0 25 20765.0 26 24315.0 27 28441.0 28 33772.0 29 40625.0 30 49314.0 31 60277.0 32 74818.0 33 94899.0 34 124403.0 35 166730.0 36 223577.0 37 283488.0 38 224091.0 39 36872.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.36743170987273 18.268385533018126 14.15896427324021 21.205218483868926 2 15.2373596764846 21.177460572879156 39.59512523564271 23.990054514993528 3 21.004150116791624 25.592718953528305 29.680367169892257 23.722763759787817 4 12.283880422039859 16.466002344665885 38.639507620164125 32.61060961313013 5 12.998777129149467 38.54130149570119 33.54969041318496 14.91023096196438 6 29.80185916434344 36.48371105853935 17.9122950507298 15.80213472638741 7 27.728873084653017 33.7744621965322 20.805402563545005 17.69126215526978 8 26.21603452388697 32.378335006661075 19.801357534751588 21.604272934700365 9 26.45727637514204 17.717808352443313 21.388799842470863 34.43611542994378 10-11 25.141199798924568 26.2794743313729 26.760824292022495 21.818501577680042 12-13 27.678482712654095 24.15893437224575 26.951441376668377 21.211141538431775 14-15 23.488676372981534 25.363488647069428 27.699128658732242 23.4487063212168 16-17 21.236080736198982 28.837722607852957 27.852593754569664 22.073602901378393 18-19 20.923228236267793 28.056060364945683 30.12066483030345 20.90004656848307 20-21 22.719349760462126 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29.180413650289626 22.78188067232455 46-47 22.779815653621004 26.9923882407402 28.82363454180022 21.404161563838578 48-49 22.532624790446036 26.898993965197487 28.629656258258372 21.938724986098098 50-51 21.327448692260205 27.413007052955617 29.498833785160407 21.760710469623774 52-53 22.17385763192551 28.01164304998689 27.89213625356887 21.922363064518727 54-55 21.410778753293904 26.975127170959606 28.593323063166213 23.020771012580273 56-57 22.521249488374494 26.98010425001487 27.959964150323707 22.538682111286935 58-59 23.524708486821076 26.7031023348302 27.747331708436136 22.024857469912593 60-61 22.346322231034424 27.00708456031105 29.35271535747863 21.293877851175893 62-63 21.86374488708594 27.283865941671248 28.98102673437692 21.871362436865894 64-65 21.422665611907064 28.411502739445076 28.225572060590082 21.940259588057778 66-67 21.989897252698476 27.354150778273674 27.608462728210547 23.047489240817303 68-69 21.617341433049738 27.45655602660331 28.17349988675516 22.752602653591794 70-71 22.602801338111718 27.27895503753587 27.7124905761106 22.405753048241817 72-73 22.297905454818203 26.713127472857167 28.918746132498264 22.07022093982636 74-75 21.406352408764 28.72626900069409 27.819116878824783 22.048261711717124 76-77 21.912906105584888 27.32490292609023 27.806104735226207 22.95608623309868 78-79 22.11910984291742 27.465052298824126 28.070967785424912 22.34487007283354 80-81 22.601412782442935 27.51403331658078 27.68652112946403 22.198032771512256 82-83 22.08101370112658 27.310078266619826 28.519339001141788 22.089569031111807 84-85 21.88693618991251 27.876727982326088 28.019566707882355 22.21676911987905 86-87 21.89699794401401 28.147464913897345 27.615111921876633 22.34042522021201 88-89 21.672016302349395 27.919648520878226 27.029113640325754 23.37922153644662 90-91 22.028303255105815 27.178702206355265 27.401889872401817 23.391104666137092 92-93 22.63541459415253 27.766625067431487 27.651702174480615 21.94625816393537 94-95 21.99362675123703 27.35914510802176 27.897906133460843 22.749322007280366 96-97 20.987641660904522 27.504863642223448 28.765279454334504 22.742215242537533 98-99 21.638193790991693 28.09626222259862 27.17841162409533 23.087132362314353 100-101 21.32737705653197 27.277137165051023 27.779694356358362 23.615791422058642 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 368.0 1 310.0 2 560.0 3 825.5 4 647.0 5 578.5 6 890.5 7 1734.0 8 2813.0 9 3348.5 10 2986.5 11 2476.5 12 2472.5 13 2608.5 14 2764.5 15 2864.0 16 2984.0 17 3158.0 18 3344.0 19 3555.0 20 3785.5 21 4352.0 22 5168.0 23 6128.0 24 7155.0 25 8459.0 26 10324.5 27 12601.0 28 14680.5 29 17064.5 30 20293.0 31 23769.0 32 27897.0 33 31199.5 34 34143.5 35 36858.5 36 39763.0 37 43209.5 38 44948.0 39 45710.5 40 46630.0 41 48431.5 42 49976.5 43 51793.0 44 54359.5 45 57059.0 46 59672.0 47 71697.0 48 100886.0 49 99090.0 50 69666.0 51 56786.5 52 51484.0 53 49395.5 54 47883.5 55 44684.5 56 41080.5 57 37051.0 58 33889.5 59 30605.5 60 25986.0 61 22000.5 62 18608.5 63 14477.5 64 10700.0 65 8376.5 66 6376.0 67 4896.0 68 3597.0 69 2448.0 70 1810.5 71 1391.5 72 1142.0 73 1066.0 74 873.5 75 674.5 76 516.0 77 333.0 78 197.0 79 122.0 80 83.5 81 57.5 82 38.5 83 24.0 84 18.0 85 13.5 86 9.0 87 7.0 88 6.5 89 3.0 90 4.5 91 4.0 92 2.5 93 5.0 94 3.5 95 1.0 96 0.5 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012715243584808036 2 0.006445515181239098 3 0.009082316846291455 4 0.035919098237268784 5 0.04634911371236478 6 0.05906435729717282 7 0.026953972576090772 8 0.025313295984502635 9 0.015352045249860396 10-11 0.04602683795330283 12-13 0.042569697992456405 14-15 0.019658821302779246 16-17 0.02282298330084208 18-19 0.030674792703442432 20-21 0.024082788540811535 22-23 0.014971173898241721 24-25 0.00377941571990838 26-27 0.002783290646444156 28-29 0.0020508457394851675 30-31 0.007295151273311524 32-33 0.0015527832027530555 34-35 0.002080143535763527 36-37 0.006592004162630894 38-39 0.006592004162630894 40-41 0.0037501179236300204 42-43 9.082316846291456E-4 44-45 0.0015527832027530555 46-47 0.00251961047993892 48-49 7.031471106806288E-4 50-51 0.0013769964250828983 52-53 9.082316846291456E-4 54-55 0.0030469708129493914 56-57 0.0024903126836605606 58-59 0.0025489082762172794 60-61 8.496360920724265E-4 62-63 0.0016113787953097745 64-65 0.003691522331073301 66-67 0.002783290646444156 68-69 0.007822511606321995 70-71 0.010459313271374353 72-73 0.004980625367321121 74-75 0.003720820127351661 76-77 0.0030762686092277508 78-79 0.03512805773775308 80-81 0.023760512781749583 82-83 0.00436537164547557 84-85 0.008379169735610826 86-87 0.007763916013765276 88-89 0.0064748129775174575 90-91 0.011807011900178892 92-93 0.014736791528014846 94-95 0.0061525372184555015 96-97 0.004277478256640492 98-99 0.02335034363385255 100-101 0.04321424951058032 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1706613.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.55249795867261 #Duplication Level Percentage of deduplicated Percentage of total 1 80.15148237050037 46.12918025512806 2 11.894534617356134 13.691203585694991 3 3.5874058617150553 6.193925055998576 4 1.4686534743791573 3.3809870434481555 5 0.7738820338651948 2.226942208714002 6 0.4492546525460265 1.5513436484147616 7 0.3068919654770032 1.2363679451653777 8 0.21424249631061343 0.9864152665262003 9 0.15782506510240618 0.8174904063420245 >10 0.8896657999055471 9.51847054142825 >50 0.06737683324204957 2.661255108861954 >100 0.033171567033686164 3.588489610860828 >500 0.0024494236654964982 0.9058526198919682 >1k 0.0025514829848921855 3.5246136888365607 >5k 4.082372775827497E-4 1.865567699415692 >10k+ 2.0411863879137484E-4 1.7218953152725358 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17687 1.03638024555069 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11628 0.6813495502495293 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9855 0.5774595646464664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7893 0.4624950120501836 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7157 0.41936865592843836 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6856 0.401731382568866 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4934 0.2891106536748519 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4781 0.2801455280136739 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4284 0.2510235185129845 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4014 0.23520270852267033 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3826 0.22418673712200715 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3362 0.1969983821756895 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3258 0.19090444054979072 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3167 0.1855722416271293 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2800 0.16406765915881338 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2078 0.12176164133286223 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2052 0.12023815592638752 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1954 0.11449578785582906 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1929 0.11303089804191109 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1853 0.10857763300760044 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1826 0.10699555200856903 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1745 0.10224930901147476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 5.859559255671907E-5 5 0.0 0.0 0.0 0.0 5.859559255671907E-5 6 0.0 0.0 0.0 0.0 5.859559255671907E-5 7 0.0 0.0 0.0 0.0 5.859559255671907E-5 8 0.0 0.0 0.0 0.0 5.859559255671907E-5 9 0.0 0.0 0.0 0.0 5.859559255671907E-5 10-11 0.0 0.0 0.0 2.9297796278359535E-5 5.859559255671907E-5 12-13 0.0 0.0 0.0 5.859559255671907E-5 5.859559255671907E-5 14-15 0.0 0.0 0.0 5.859559255671907E-5 5.859559255671907E-5 16-17 0.0 0.0 0.0 5.859559255671907E-5 5.859559255671907E-5 18-19 0.0 0.0 0.0 5.859559255671907E-5 5.859559255671907E-5 20-21 0.0 0.0 0.0 5.859559255671907E-5 5.859559255671907E-5 22-23 0.0 0.0 0.0 1.1719118511343814E-4 5.859559255671907E-5 24-25 0.0 0.0 0.0 1.1719118511343814E-4 5.859559255671907E-5 26-27 0.0 0.0 0.0 2.3438237022687628E-4 5.859559255671907E-5 28-29 0.0 0.0 0.0 6.152537218455502E-4 5.859559255671907E-5 30-31 0.0 0.0 0.0 0.0016699743878664935 5.859559255671907E-5 32-33 0.0 0.0 0.0 0.00249031268366056 5.859559255671907E-5 34-35 0.0 0.0 0.0 0.003955202497578537 5.859559255671907E-5 36-37 0.0 0.0 0.0 0.006123239422177143 5.859559255671907E-5 38-39 0.0 0.0 0.0 0.009521783790466847 5.859559255671907E-5 40-41 0.0 0.0 0.0 0.013594177473158824 5.859559255671907E-5 42-43 0.0 0.0 0.0 0.01702201963772689 5.859559255671907E-5 44-45 0.0 0.0 0.0 0.019219354358603856 8.78933888350786E-5 46-47 0.0 0.0 0.0 0.023057365671068954 1.1719118511343814E-4 48-49 0.0 0.0 0.0 0.029063413908132656 1.757867776701572E-4 50-51 0.0 0.0 0.0 0.035538226885650115 1.757867776701572E-4 52-53 0.0 0.0 0.0 0.04716945200815885 1.757867776701572E-4 54-55 0.0 0.0 0.0 0.056896320372574215 1.757867776701572E-4 56-57 0.0 0.0 0.0 0.08112559789477755 1.757867776701572E-4 58-59 0.0 0.0 0.0 0.12070692066684129 1.757867776701572E-4 60-61 0.0 0.0 0.0 0.15480955553485176 1.757867776701572E-4 62-63 0.0 0.0 0.0 0.17648992478083783 1.757867776701572E-4 64-65 0.0 0.0 0.0 0.21545599383105601 1.757867776701572E-4 66-67 0.0 0.0 0.0 0.28600508726934576 1.757867776701572E-4 68-69 0.0 0.0 0.0 0.3822776458400352 1.757867776701572E-4 70-71 0.0 0.0 0.0 0.4720167958406505 1.757867776701572E-4 72-73 0.0 0.0 0.0 0.5648615122467718 2.3438237022687628E-4 74-75 0.0 0.0 0.0 0.6762810314933732 2.3438237022687628E-4 76-77 0.0 0.0 0.0 0.7583734566653366 2.3438237022687628E-4 78-79 0.0 0.0 0.0 0.865163924099957 2.3438237022687628E-4 80-81 0.0 0.0 0.0 1.0056175594584127 2.636801665052358E-4 82-83 0.0 0.0 0.0 1.1363443264524529 2.9297796278359535E-4 84-85 0.0 0.0 0.0 1.3026679159246999 2.9297796278359535E-4 86-87 0.0 0.0 0.0 1.5432907167588668 2.9297796278359535E-4 88-89 0.0 0.0 0.0 1.8440325955562273 2.9297796278359535E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 145 2.3914254E-8 29.495497 2 CTATCGG 90 8.923734E-4 26.401838 1 GTACATG 10740 0.0 26.372337 1 TACATGG 10850 0.0 25.446474 2 ACATGGG 10850 0.0 24.263937 3 GAGTACT 8390 0.0 23.387993 12-13 TACCTGG 1605 0.0 22.50193 2 GTACCTG 1710 0.0 22.233128 1 GTACTTT 9395 0.0 20.960764 14-15 GGGTCGA 115 0.0037140665 20.661703 6 AGTACTT 8785 0.0 20.659815 12-13 CATGGGG 3495 0.0 20.395073 4 CATGGGA 7350 0.0 20.365938 4 ATAACGC 215 1.0749591E-6 19.892313 3 GTATAGG 480 0.0 19.80138 1 ATGGGAT 2730 0.0 18.973396 5 ATGGGAG 2815 0.0 18.73811 5 ACTTTTT 10810 0.0 18.656002 16-17 TAACGCA 205 1.5842235E-5 18.544594 4 GTACACG 320 8.229108E-9 17.821241 1 >>END_MODULE