##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936386_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1706613 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.451493103591734 31.0 31.0 34.0 30.0 34.0 2 31.660816482705805 31.0 31.0 34.0 30.0 34.0 3 31.928931749611657 31.0 31.0 34.0 30.0 34.0 4 35.734212735986425 37.0 35.0 37.0 35.0 37.0 5 35.56134460478152 37.0 35.0 37.0 33.0 37.0 6 35.68582859734457 37.0 35.0 37.0 33.0 37.0 7 35.620628109594854 37.0 35.0 37.0 33.0 37.0 8 35.60810681742141 37.0 35.0 37.0 33.0 37.0 9 37.21479269172331 39.0 37.0 39.0 33.0 39.0 10-11 37.1002582307764 39.0 37.0 39.0 33.0 39.0 12-13 36.97989409432601 39.0 37.0 39.0 33.0 39.0 14-15 38.016339673962406 40.0 37.0 41.0 33.0 41.0 16-17 37.96027834078376 40.0 37.0 41.0 33.0 41.0 18-19 38.0773388577258 40.0 37.0 41.0 33.0 41.0 20-21 38.172588923206376 40.0 37.0 41.0 33.0 41.0 22-23 38.082516657262076 40.0 37.0 41.0 33.0 41.0 24-25 37.95645204858981 40.0 37.0 41.0 33.0 41.0 26-27 37.731695176352225 39.5 37.0 41.0 32.5 41.0 28-29 37.644338816122925 39.0 37.0 41.0 32.5 41.0 30-31 37.60252295042872 39.0 36.5 41.0 33.0 41.0 32-33 37.37494470040953 39.0 36.0 41.0 32.0 41.0 34-35 37.15456784871556 39.0 36.0 41.0 31.0 41.0 36-37 36.89103446416968 39.0 35.0 41.0 31.0 41.0 38-39 36.7169135591959 39.0 35.0 41.0 31.0 41.0 40-41 36.50084055377523 39.0 35.0 40.5 30.0 41.0 42-43 36.209019853944625 39.0 35.0 40.0 30.0 41.0 44-45 35.86948945074249 38.5 35.0 40.0 29.5 41.0 46-47 35.38784862180236 38.0 35.0 40.0 27.5 41.0 48-49 34.8600127855583 38.0 34.0 40.0 25.0 41.0 50-51 34.63038691255721 38.0 34.0 40.0 24.0 41.0 52-53 34.372106329906075 38.0 34.0 40.0 24.0 41.0 54-55 34.110877510015456 38.0 33.0 40.0 23.0 41.0 56-57 33.79939945377188 37.5 33.0 40.0 22.5 41.0 58-59 33.431012186125386 37.0 33.0 40.0 21.5 41.0 60-61 33.00786557936685 36.5 32.0 39.5 20.0 41.0 62-63 32.74548008247916 36.0 32.0 39.0 20.0 40.0 64-65 32.31958182669416 36.0 31.0 39.0 18.0 40.0 66-67 31.867932565848264 35.0 31.0 38.5 16.5 40.0 68-69 31.403243734812754 35.0 30.5 38.0 13.0 40.0 70-71 30.8904666728778 34.5 30.0 37.0 9.5 39.0 72-73 30.457829630970817 34.0 29.0 37.0 7.5 39.0 74-75 30.100632949590796 34.0 29.0 36.0 7.0 39.0 76-77 28.7028977864343 32.5 27.5 35.0 4.0 37.0 78-79 29.48942290958759 34.0 29.0 35.0 2.0 37.0 80-81 29.297543731355617 34.0 29.0 35.0 2.0 37.0 82-83 28.936695958603387 34.0 29.0 35.0 2.0 36.5 84-85 28.468548229739255 33.5 28.0 35.0 2.0 36.0 86-87 28.12499787590977 33.0 27.5 35.0 2.0 36.0 88-89 27.67467228949973 33.0 26.5 35.0 2.0 35.0 90-91 27.16317407637232 33.0 26.0 35.0 2.0 35.0 92-93 26.70218555700677 32.5 25.0 34.5 2.0 35.0 94-95 26.141652501182165 32.0 24.5 34.0 2.0 35.0 96-97 25.426436456302632 32.0 21.5 34.0 2.0 35.0 98-99 24.572483626926548 32.0 10.0 34.0 2.0 35.0 100-101 21.78418979581194 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38915572449410263 1101 2 0.41690924310179867 1101 3 0.2514602928897318 1101 4 0.1366994695868371 1101 5 0.18538661062296313 1101 6 0.18114243361515037 1101 7 0.170564641696906 1101 8 0.28243560674427215 1101 9 0.3394565904320146 1101 10-11 0.2052196272627782 1101 12-13 0.22675897470490725 1101 14-15 0.3846363635526657 1101 16-17 0.30154246473385626 1101 18-19 0.24793513637828113 1101 20-21 0.19310254021743134 1101 22-23 0.057863377489489665 1101 24-25 0.047879401416587086 1101 26-27 0.139276950849748 1101 28-29 0.0682866908227382 1101 30-31 0.09728759147076715 1101 32-33 0.18991616298060165 1101 34-35 0.14057594039821453 1101 36-37 0.22117265030397704 1101 38-39 0.3923853683561802 1101 40-41 0.3912585836570841 1101 42-43 0.2973103163620081 1101 44-45 0.3237166143257113 1101 46-47 0.25269394242199184 1101 48-49 0.15076907451529564 1101 50-51 -0.008301831135028692 1101 52-53 0.013666668848507868 1101 54-55 0.13957061894819134 1101 56-57 0.03648906915013583 1101 58-59 0.039350978396932135 1101 60-61 0.03600317197692249 1101 62-63 -0.20429383922226663 1101 64-65 0.1367819216126307 1101 66-67 -0.13223902924686826 1101 68-69 -0.16747447497666457 1101 70-71 -0.20140750935772544 1101 72-73 -0.16883451032589747 1101 74-75 -0.14248691259320978 1101 76-77 0.13973259211322997 1101 78-79 0.13026528219059585 1101 80-81 0.1583545143539098 1101 82-83 0.1754963902851614 1101 84-85 0.14733815583485566 1101 86-87 0.018019538212389108 1101 88-89 -0.14213095476525694 1101 90-91 -0.036474264698838965 1101 92-93 -0.14138527291994762 1101 94-95 -0.19888984612165928 1101 96-97 0.19799871828151083 1101 98-99 0.22135401350224626 1101 100-101 0.04972350164039696 1102 1 0.5051725466540375 1102 2 0.5458467885198246 1102 3 0.3598660693856459 1102 4 0.24245068938525804 1102 5 0.1827805750468272 1102 6 0.20058022051038193 1102 7 0.1883216795162994 1102 8 0.10504013026686465 1102 9 0.1641971716924786 1102 10-11 0.2366971405440239 1102 12-13 0.31701333822064015 1102 14-15 0.4558354671531575 1102 16-17 0.4157719041907839 1102 18-19 0.25475658542207924 1102 20-21 0.3317135610566808 1102 22-23 0.1514453872117656 1102 24-25 0.3049564326461365 1102 26-27 0.10047218404451286 1102 28-29 0.32448244308066165 1102 30-31 0.33463063128517234 1102 32-33 0.4843702552294076 1102 34-35 0.46972912270673106 1102 36-37 0.32847682808536405 1102 38-39 0.11630470142772253 1102 40-41 0.21276799675462144 1102 42-43 0.17220481113300679 1102 44-45 0.12508891576148784 1102 46-47 0.4937254337581507 1102 48-49 0.3223501973166094 1102 50-51 0.267477835581289 1102 52-53 0.2559811489051924 1102 54-55 0.346700797731593 1102 56-57 0.40917416030213616 1102 58-59 0.3937424783991972 1102 60-61 0.5763236596254728 1102 62-63 0.5437850628914518 1102 64-65 0.21222069924146325 1102 66-67 0.3673372572398854 1102 68-69 0.3384927766516128 1102 70-71 0.2644279792161157 1102 72-73 0.18409232885028004 1102 74-75 -0.11664461977839835 1102 76-77 -0.021169370396872722 1102 78-79 0.3225829781820728 1102 80-81 0.4451467830210021 1102 82-83 0.5147814597719211 1102 84-85 0.575142958748831 1102 86-87 0.3465653602053216 1102 88-89 0.18667975869833597 1102 90-91 0.358666232157443 1102 92-93 0.5149940697204123 1102 94-95 0.2948692656935705 1102 96-97 0.5012529057671244 1102 98-99 0.04480398935452712 1102 100-101 0.06509341312884587 1103 1 0.3536723392946257 1103 2 0.4329390450027901 1103 3 0.16272887779206258 1103 4 0.06006017189866952 1103 5 0.09257895872904953 1103 6 -0.030960991901650914 1103 7 0.09962586166254539 1103 8 0.10733628293550623 1103 9 -0.02646872343794371 1103 10-11 0.050749622017384866 1103 12-13 0.14566850928672181 1103 14-15 0.05120478403959794 1103 16-17 0.023785259874060216 1103 18-19 0.048282384841478176 1103 20-21 0.04369907086771718 1103 22-23 -0.08051678652285688 1103 24-25 -0.0750892678487034 1103 26-27 0.00659604492479815 1103 28-29 0.12936768430154189 1103 30-31 0.16618951267341942 1103 32-33 0.015339139375207367 1103 34-35 -0.03980642477640828 1103 36-37 0.08191820725345877 1103 38-39 0.15107105839423696 1103 40-41 0.0469230777116465 1103 42-43 0.03635236852597501 1103 44-45 0.030616211208482014 1103 46-47 0.13781742751348958 1103 48-49 0.2588172000285667 1103 50-51 -0.04783780127266368 1103 52-53 -0.01885855466855446 1103 54-55 -0.1369320577703732 1103 56-57 -0.10735705326068512 1103 58-59 -0.05651406102189327 1103 60-61 -0.1345426793192317 1103 62-63 -0.2195904335226473 1103 64-65 -0.20191282610741723 1103 66-67 -0.1247111606403557 1103 68-69 -0.17608277165661335 1103 70-71 -0.19089040976409066 1103 72-73 -0.14866633301925347 1103 74-75 -0.17548027495815077 1103 76-77 -0.3920977706027138 1103 78-79 -0.11807111048959129 1103 80-81 -0.12468489216078993 1103 82-83 -0.1712364903879937 1103 84-85 -0.15077770076267072 1103 86-87 -0.15749348318512446 1103 88-89 0.07652305577815355 1103 90-91 -0.11699848504122556 1103 92-93 0.20077874054006273 1103 94-95 0.19381832801647292 1103 96-97 -0.2584862013497542 1103 98-99 -0.6193770332422197 1103 100-101 -0.36447875337533375 1104 1 0.3292748393503899 1104 2 0.370673606631712 1104 3 0.13826123611045915 1104 4 0.10815536686717309 1104 5 -0.006087085172154616 1104 6 0.145844559884047 1104 7 0.06812765296427159 1104 8 0.08619217713918914 1104 9 0.05810877471647302 1104 10-11 0.03312053098711942 1104 12-13 -0.03969124392185819 1104 14-15 0.17436668756273122 1104 16-17 0.10127830818290562 1104 18-19 0.08247486935587034 1104 20-21 -0.009304030149557718 1104 22-23 0.01632261604154195 1104 24-25 -0.01217159194276718 1104 26-27 0.12548212426236205 1104 28-29 0.09385110653034445 1104 30-31 0.0726718991694355 1104 32-33 0.26596403238218613 1104 34-35 0.2706742288693391 1104 36-37 0.3934949263239744 1104 38-39 0.2655331021739755 1104 40-41 0.3640340067349257 1104 42-43 0.3494716683340684 1104 44-45 0.5839555088290069 1104 46-47 0.6132193114506777 1104 48-49 0.6586652800127197 1104 50-51 0.6998392575687262 1104 52-53 0.42037935132378834 1104 54-55 0.5382164290627287 1104 56-57 0.837647927877633 1104 58-59 0.9018300779909723 1104 60-61 0.6588905759803012 1104 62-63 0.6097623786100996 1104 64-65 0.5959365368524274 1104 66-67 0.647231311310339 1104 68-69 0.5609628422408015 1104 70-71 0.5171960725794094 1104 72-73 0.4411165688976091 1104 74-75 0.234101113240893 1104 76-77 0.6140523966759268 1104 78-79 0.7048316179702461 1104 80-81 0.7781263402994085 1104 82-83 0.6594327558463213 1104 84-85 0.670978169979815 1104 86-87 0.5989525021678865 1104 88-89 0.5105130967264131 1104 90-91 0.24725548502141592 1104 92-93 0.369840752016966 1104 94-95 0.5220131296268846 1104 96-97 0.1495585184420669 1104 98-99 0.4527563532304235 1104 100-101 0.6896083196637122 1105 1 0.37247686763369714 1105 2 0.36191477043621845 1105 3 0.12058269913378439 1105 4 0.014857648204902318 1105 5 -0.14990884293573714 1105 6 -0.015026171793287801 1105 7 0.1461875939948385 1105 8 -0.050445186520057916 1105 9 0.06234843836519133 1105 10-11 0.06984669082879691 1105 12-13 0.13551286972229803 1105 14-15 0.21452805451622936 1105 16-17 0.15556155491123747 1105 18-19 -0.08551759405812476 1105 20-21 0.019016554264538854 1105 22-23 -0.027889625806871265 1105 24-25 -0.1799093976322439 1105 26-27 0.05971255823867949 1105 28-29 0.09222789735133574 1105 30-31 0.11718182066645255 1105 32-33 0.05494483498424074 1105 34-35 0.004687843036968786 1105 36-37 0.2895679866364347 1105 38-39 0.10305612959203359 1105 40-41 0.1498969223173603 1105 42-43 0.17934347368898074 1105 44-45 0.10769051729176482 1105 46-47 0.3080975380281785 1105 48-49 0.23116924684789808 1105 50-51 0.24981550198455693 1105 52-53 0.24215181184114698 1105 54-55 0.34272854708282097 1105 56-57 0.3098708626783093 1105 58-59 0.43989820120362566 1105 60-61 0.41200218340230066 1105 62-63 0.15344659982509512 1105 64-65 0.20419118816374038 1105 66-67 0.2633603108127822 1105 68-69 -0.026497389201118438 1105 70-71 0.13579495807108088 1105 72-73 0.1828066571272906 1105 74-75 0.08773157575597068 1105 76-77 0.13339703114722923 1105 78-79 0.370806192814765 1105 80-81 0.20305161308780129 1105 82-83 0.35885193314955544 1105 84-85 0.4179679713788538 1105 86-87 0.23907116785760607 1105 88-89 0.23614411612155095 1105 90-91 0.15810711068899153 1105 92-93 0.3949281453105691 1105 94-95 0.5367215505405625 1105 96-97 0.5820424226309058 1105 98-99 0.7375870098518682 1105 100-101 0.6547244868752351 1106 1 0.3909851546254792 1106 2 0.3130526312382962 1106 3 0.06771192041046348 1106 4 -0.02768667458729368 1106 5 -0.15249808515739005 1106 6 0.014632965801617104 1106 7 0.0015741658969830041 1106 8 0.06105405664967378 1106 9 -0.05834991600573858 1106 10-11 -0.04509534000455062 1106 12-13 -0.017982552789511885 1106 14-15 0.0746675641076493 1106 16-17 -0.01008347262126108 1106 18-19 -0.12177406852585904 1106 20-21 -0.01596082800721632 1106 22-23 0.00925390386642988 1106 24-25 -0.04371427599997446 1106 26-27 -0.1224004748253833 1106 28-29 0.0258464178435176 1106 30-31 0.03838102340651517 1106 32-33 -0.040768312721354505 1106 34-35 -0.21678090897225388 1106 36-37 -0.09562974276378355 1106 38-39 -0.14161173143889272 1106 40-41 -0.04214235657432397 1106 42-43 -0.14255485714337368 1106 44-45 -0.148428147592071 1106 46-47 -0.25632648089857213 1106 48-49 -0.41713570398810873 1106 50-51 -0.2228939285892011 1106 52-53 -0.12525468502091996 1106 54-55 -0.08188810667032698 1106 56-57 -0.24820346470172439 1106 58-59 -0.3834099916338971 1106 60-61 -0.2452141850547136 1106 62-63 -0.4734644102300152 1106 64-65 -0.14625746125656747 1106 66-67 -0.3793297113418035 1106 68-69 -0.29101910407280585 1106 70-71 -0.26710409962347015 1106 72-73 -0.1297560715155477 1106 74-75 -0.14607884943116645 1106 76-77 -0.1282259529544305 1106 78-79 -0.14860576804582948 1106 80-81 -0.1790460545236776 1106 82-83 -0.11919535757243338 1106 84-85 9.185305832524193E-4 1106 86-87 0.08399179603218343 1106 88-89 0.1282459465719583 1106 90-91 0.24336902627054258 1106 92-93 0.5424975159699663 1106 94-95 0.40625796066955644 1106 96-97 0.2249702823759243 1106 98-99 0.25607758843199235 1106 100-101 0.0926766643560164 1107 1 0.5018130210632812 1107 2 0.5216487004855708 1107 3 0.36830158866979446 1107 4 0.1871593985037734 1107 5 0.061429783186937925 1107 6 0.17689874825289564 1107 7 0.1737141856632931 1107 8 0.17637331576360538 1107 9 0.27856603535708757 1107 10-11 0.2804777384536834 1107 12-13 0.33236529490289257 1107 14-15 0.4211256492602189 1107 16-17 0.437704010642868 1107 18-19 0.32428786017151623 1107 20-21 0.2624363296786143 1107 22-23 0.33425518000497334 1107 24-25 0.2842953045717209 1107 26-27 0.29219679737466464 1107 28-29 0.37679451190752644 1107 30-31 0.3416885592660108 1107 32-33 0.48302982614589496 1107 34-35 0.2687244634172714 1107 36-37 0.41527939678673675 1107 38-39 0.474235972034478 1107 40-41 0.59109127395066 1107 42-43 0.44636878303106897 1107 44-45 0.22563389057646788 1107 46-47 0.4936389249842179 1107 48-49 0.4921609806961982 1107 50-51 0.434031137170372 1107 52-53 0.24200876348058387 1107 54-55 0.2398205782673628 1107 56-57 0.4637014934193999 1107 58-59 0.4670543997956216 1107 60-61 0.24167756580122557 1107 62-63 0.2693643061468052 1107 64-65 0.2570424485219718 1107 66-67 0.2932574181705796 1107 68-69 0.36390457669206455 1107 70-71 0.20134176042933305 1107 72-73 0.2740383035117411 1107 74-75 0.4924945752757388 1107 76-77 0.504822099350644 1107 78-79 0.5397207622902869 1107 80-81 0.5073793368818542 1107 82-83 0.2357690366246068 1107 84-85 0.3424920801364806 1107 86-87 0.6457372214598713 1107 88-89 0.7469800294342477 1107 90-91 0.651838527174295 1107 92-93 0.7607181298817842 1107 94-95 0.7560964292049022 1107 96-97 0.8260778168824032 1107 98-99 1.0197013667983974 1107 100-101 1.0340580858698054 1108 1 0.263722503626056 1108 2 0.2488213118450986 1108 3 -0.059417942694146575 1108 4 0.051065188331818945 1108 5 -0.07418750435292765 1108 6 -0.024507490378091745 1108 7 -0.03439407100813696 1108 8 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64-65 -1.023149657828263 2316 66-67 -0.9869225634764511 2316 68-69 -1.241673510012749 2316 70-71 -1.148846682984253 2316 72-73 -1.1497432595369297 2316 74-75 -1.3409526310011977 2316 76-77 -1.2592988192509047 2316 78-79 -1.2376920217415197 2316 80-81 -1.0471277981869136 2316 82-83 -0.9656943937815079 2316 84-85 -1.1081018814209784 2316 86-87 -0.9894942731586731 2316 88-89 -1.1541930036942745 2316 90-91 -1.329913794493656 2316 92-93 -1.4219347807063976 2316 94-95 -1.521894299013283 2316 96-97 -1.511292900810684 2316 98-99 -2.101403997723356 2316 100-101 -2.4069756535630873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 25.0 5 60.0 6 134.0 7 375.0 8 818.0 9 1586.0 10 2662.0 11 4337.0 12 6258.0 13 8389.0 14 10758.0 15 14431.0 16 18216.0 17 23079.0 18 20015.0 19 12929.0 20 11232.0 21 11740.0 22 12438.0 23 13804.0 24 16297.0 25 19310.0 26 23604.0 27 28861.0 28 35406.0 29 44046.0 30 53899.0 31 65882.0 32 81245.0 33 102384.0 34 132910.0 35 177205.0 36 244377.0 37 307544.0 38 188387.0 39 11958.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79437381314849 19.335783569208505 14.963729048203565 20.906113569439437 2 15.50385598087246 22.307318159443053 38.65497761421439 23.5338482454701 3 21.289291628528936 25.67168307223882 30.063333048154917 22.97569225107733 4 12.765886375822996 17.27364093728665 37.94516574221394 32.01530694467641 5 12.75772538941166 38.96894023425346 33.50472544156174 14.768608934773145 6 29.857325591683644 37.26158185833578 17.133116881214427 15.747975668766145 7 27.51619728667249 34.128299737550336 20.550997795047852 17.804505180729315 8 26.493411218594957 32.723470406003 19.329103903462588 21.454014471939452 9 26.167638319104764 17.662549376136436 21.248043559406355 34.921768745352445 10-11 25.320892660376014 26.622393621493885 26.485103625235222 21.57161009289488 12-13 27.83017209555786 24.354128945687652 26.91514171368971 20.90055724506478 14-15 23.323125981109953 25.66489883763923 27.79109850915234 23.220876672098477 16-17 21.0458961697819 28.919122261461737 28.06342152555969 21.971560043196668 18-19 20.68817007722313 27.961377301122166 30.68657041754633 20.663882204108372 20-21 22.55092396460123 25.482725140380392 30.458633562500697 21.507717332517686 22-23 21.56197446369661 25.91085146403061 30.783990296439097 21.74318377583368 24-25 22.381729191093704 25.95031796898301 30.827961582385694 20.83999125753759 26-27 21.833977591873495 25.339693298949438 32.074553516233614 20.75177559294345 28-29 20.660923126684256 27.43747410807254 31.136731057363328 20.764871707879877 30-31 21.332845036585635 25.856484702883638 30.95796497447429 21.85270528605644 32-33 21.349439484153145 26.188287149593357 31.10600725556136 21.356266110692136 34-35 22.601464663155603 25.660493002477438 30.783737762245956 20.954304572121007 36-37 22.18347318049029 25.383408957933344 31.53907658393422 20.894041277642142 38-39 21.513403321964795 26.309459598426237 31.393777314741705 20.783359764867264 40-41 21.62020894183387 26.59556421238972 30.614077571054843 21.170149274721567 42-43 21.206395157190595 26.855851117599578 29.689046982107563 22.248706743102264 44-45 21.915008815892868 25.96431335943236 29.68875708508904 22.43192073958573 46-47 22.825772000690268 27.01005544101581 29.19889592599505 20.96527663229888 48-49 22.69302183440148 26.87859095313481 28.905361601234368 21.523025611229336 50-51 21.435674080602084 27.439783992321605 29.707765002584075 21.416776924492236 52-53 22.335902992062497 28.056518819488314 28.03524823301781 21.572329955431382 54-55 21.576787922809633 27.044697594108026 28.58619601495848 22.792318468123856 56-57 22.699454807119853 26.97480065066872 28.214991397859546 22.110753144351886 58-59 23.807619434353697 26.664064997203496 27.82523578361962 21.703079784823185 60-61 22.328875049186255 27.143048425644302 29.47756066646606 21.05051585870338 62-63 22.06180166370163 27.157737572045686 29.167369837262886 21.6130909269898 64-65 21.614573184293043 28.40372622212637 28.371610690604065 21.61008990297652 66-67 22.153445439456686 27.322710645784593 27.793304396105047 22.73053951865367 68-69 21.881905132452562 27.424732408264095 28.19388835534284 22.4994741039405 70-71 22.832529980698443 27.384510001769602 27.7845749262568 21.998385091275157 72-73 22.586022724542705 26.641306494208116 28.977600662833343 21.795070118415833 74-75 21.631061054849575 28.814529128747996 27.77627382423549 21.77813599216694 76-77 22.122491647124864 27.331304736088992 27.76342173142122 22.78278188536492 78-79 22.348335459583566 27.470645147490664 28.092316960327512 22.088702432598257 80-81 22.868849432742195 27.463318266234637 27.74174606048992 21.926086240533248 82-83 22.403807498965673 27.246659970394195 28.44539627360641 21.904136257033723 84-85 21.923934738250974 27.918929118502277 28.096575293498553 22.060560849748196 86-87 22.03626816997811 28.06947736682186 27.443501293617473 22.450753169582555 88-89 21.844215346013097 27.971203110617342 26.973229009179406 23.21135253419015 90-91 22.23371924047005 27.13725124755346 27.39964114064878 23.229388371327705 92-93 22.786939280163253 27.814545864725538 27.56090808010817 21.837606775003042 94-95 22.234528216032306 27.45804168860503 27.720364456656675 22.587065638705983 96-97 21.197612931448987 27.559788788911682 28.54221159844343 22.7003866811959 98-99 21.823565089234176 28.077546272557598 27.00324940740068 23.095639230807546 100-101 21.34109768900638 27.573962139096786 27.411459183365512 23.673480988531324 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 832.0 1 630.0 2 810.5 3 1085.5 4 782.0 5 620.0 6 1008.5 7 1940.0 8 2930.0 9 3445.5 10 3169.5 11 2662.5 12 2572.5 13 2709.5 14 2943.5 15 3148.0 16 3340.5 17 3549.0 18 3766.0 19 4044.0 20 4388.5 21 4976.0 22 5654.0 23 6414.0 24 7553.0 25 8868.0 26 10660.0 27 13057.5 28 15286.5 29 17799.0 30 21117.5 31 24420.0 32 28258.5 33 31556.0 34 34268.0 35 36553.5 36 39476.5 37 42977.5 38 44603.0 39 45194.0 40 45960.0 41 48122.5 42 49841.0 43 51534.0 44 53859.0 45 55658.5 46 58001.0 47 72215.5 48 104134.5 49 100743.5 50 68526.0 51 55119.0 52 50012.0 53 48372.5 54 47001.0 55 44136.5 56 40694.5 57 36834.5 58 33805.5 59 30347.0 60 25688.5 61 21734.5 62 18227.5 63 14105.5 64 10443.5 65 8088.5 66 6091.0 67 4650.0 68 3413.0 69 2351.0 70 1730.5 71 1341.0 72 1114.0 73 1008.5 74 830.5 75 709.0 76 542.5 77 319.0 78 201.5 79 129.5 80 100.5 81 89.0 82 58.0 83 30.0 84 14.0 85 9.0 86 7.5 87 6.0 88 2.5 89 1.5 90 1.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0427671651393724 2 0.010137037512312399 3 0.004629051811980806 4 0.011601927326230376 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004101691478970335 10-11 3.808713516186739E-4 12-13 1.757867776701572E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013476986288045384 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.9297796278359535E-5 32-33 0.0035157355534031444 34-35 5.273603330104716E-4 36-37 0.002783290646444156 38-39 1.4648898139179767E-4 40-41 0.0036036289422382226 42-43 0.009697570568137005 44-45 0.0021094413320418863 46-47 0.0014355920176396173 48-49 0.009053019050013095 50-51 4.1016914789703343E-4 52-53 0.0018164633692582912 54-55 0.009170210235126534 56-57 0.003574331145959863 58-59 9.668272771858646E-4 60-61 0.005244305533826357 62-63 0.003339948775732987 64-65 0.016025894564262667 66-67 0.0026660994613307175 68-69 1.1719118511343814E-4 70-71 8.789338883507861E-4 72-73 0.0 74-75 0.0 76-77 8.789338883507861E-4 78-79 0.009726868364415366 80-81 0.01441451576895289 82-83 0.01119175817833334 84-85 0.007676022624930198 86-87 0.009258103623961612 88-89 0.026748888002142257 90-91 0.021885453819934574 92-93 0.0026660994613307175 94-95 0.0074709380509816814 96-97 0.009873357345807163 98-99 0.008672147698394422 100-101 0.01069369564160123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1706613.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.30672338225558 #Duplication Level Percentage of deduplicated Percentage of total 1 78.60248713166979 43.47246012948565 2 12.80783873461251 14.167191880395047 3 3.8231260018212976 6.343337167147177 4 1.607334390295823 3.555855940275101 5 0.8243758446680254 2.2796763402033884 6 0.5088465808546858 1.6885582254801994 7 0.32856444243513067 1.2720275911703365 8 0.23718379900679867 1.0494287009937215 9 0.17402104947591074 0.8662080641448607 >10 0.9707283655157212 9.946749375589922 >50 0.07118030710433663 2.690822546003739 >100 0.03783139096567575 3.8671347987015667 >500 0.003294111619727951 1.2482127423574174 >1k 0.00233775663335532 3.175040887323296 >5k 6.375699909150872E-4 2.5291015186500827 >10k+ 2.1252333030502907E-4 1.848194092078538 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 20739 1.215213994033797 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10709 0.6275002006899044 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9896 0.579861983941292 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9288 0.5442358636668067 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6366 0.3730195422160736 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6200 0.36329267385165825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5986 0.35075321704452034 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5298 0.3104394493654976 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4965 0.2909271170441102 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4704 0.2756336673868065 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4195 0.2458085107754365 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3825 0.22412814152945043 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3569 0.20912766983493036 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3269 0.19154899206791465 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3105 0.1819393148886127 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2611 0.15299309216559348 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2337 0.13693789980505247 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2056 0.1204725382966144 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2033 0.11912483966780986 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1868 0.10945656689595122 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1826 0.10699555200856903 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1774 0.10394858119561962 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1720 0.10078441919755679 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.859559255671907E-5 7 0.0 0.0 0.0 0.0 5.859559255671907E-5 8 0.0 0.0 0.0 0.0 5.859559255671907E-5 9 0.0 0.0 0.0 0.0 5.859559255671907E-5 10-11 0.0 0.0 0.0 0.0 5.859559255671907E-5 12-13 0.0 0.0 0.0 0.0 5.859559255671907E-5 14-15 0.0 0.0 0.0 0.0 5.859559255671907E-5 16-17 0.0 0.0 0.0 0.0 5.859559255671907E-5 18-19 0.0 0.0 0.0 0.0 1.1719118511343814E-4 20-21 0.0 0.0 0.0 0.0 1.1719118511343814E-4 22-23 0.0 0.0 0.0 5.859559255671907E-5 1.4648898139179767E-4 24-25 0.0 0.0 0.0 5.859559255671907E-5 2.3438237022687628E-4 26-27 0.0 0.0 0.0 1.757867776701572E-4 2.3438237022687628E-4 28-29 0.0 0.0 0.0 5.566581292888312E-4 2.3438237022687628E-4 30-31 0.0 0.0 0.0 0.0016113787953097743 2.3438237022687628E-4 32-33 0.0 0.0 0.0 0.0024317170911038414 2.3438237022687628E-4 34-35 0.0 0.0 0.0 0.004013798090135256 2.3438237022687628E-4 36-37 0.0 0.0 0.0 0.006181835014733862 2.3438237022687628E-4 38-39 0.0 0.0 0.0 0.00946318819791013 2.3438237022687628E-4 40-41 0.0 0.0 0.0 0.013359795102931948 2.3438237022687628E-4 42-43 0.0 0.0 0.0 0.01693412624889181 2.3438237022687628E-4 44-45 0.0 0.0 0.0 0.019131460969768775 2.3438237022687628E-4 46-47 0.0 0.0 0.0 0.023028067874790593 2.3438237022687628E-4 48-49 0.0 0.0 0.0 0.02891692492674086 2.3438237022687628E-4 50-51 0.0 0.0 0.0 0.035421035700536674 2.3438237022687628E-4 52-53 0.0 0.0 0.0 0.046964367434210334 2.3438237022687628E-4 54-55 0.0 0.0 0.0 0.0566912357986257 2.3438237022687628E-4 56-57 0.0 0.0 0.0 0.08092051332082903 2.3438237022687628E-4 58-59 0.0 0.0 0.0 0.12058972948172784 2.636801665052358E-4 60-61 0.0 0.0 0.0 0.1547216621460167 2.9297796278359535E-4 62-63 0.0 0.0 0.0 0.17669500935478635 2.9297796278359535E-4 64-65 0.0 0.0 0.0 0.2153973982384993 2.9297796278359535E-4 66-67 0.0 0.0 0.0 0.28600508726934576 3.222757590619549E-4 68-69 0.0 0.0 0.0 0.3823655392288703 3.515735553403144E-4 70-71 0.0 0.0 0.0 0.47219258261832064 3.515735553403144E-4 72-73 0.0 0.0 0.0 0.5647736188579368 4.6876474045375255E-4 74-75 0.0 0.0 0.0 0.6749040350682902 4.6876474045375255E-4 76-77 0.0 0.0 0.0 0.7568792690551402 4.6876474045375255E-4 78-79 0.0 0.0 0.0 0.8638162254711526 4.6876474045375255E-4 80-81 0.0 0.0 0.0 1.0053831770881858 4.6876474045375255E-4 82-83 0.0 0.0 0.0 1.1360513484896693 4.6876474045375255E-4 84-85 0.0 0.0 0.0 1.3015838974624008 4.6876474045375255E-4 86-87 0.0 0.0 0.0 1.5421188049077323 4.6876474045375255E-4 88-89 0.0 0.0 0.0 1.8413664960948968 4.6876474045375255E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 95 3.4898334E-5 30.002508 2 TATAACG 105 6.903837E-5 27.145126 2 TACCTGG 1455 0.0 26.771997 2 GTACCTG 1460 0.0 26.632807 1 GTACATG 10295 0.0 23.734287 1 TACATGG 10495 0.0 23.174877 2 GAGTACT 10725 0.0 22.604822 12-13 ACATGGG 10625 0.0 22.130613 3 ATCACGC 135 3.7955935E-4 21.112257 3 GTACTTT 11495 0.0 20.946026 14-15 TAACGCA 165 6.322374E-5 20.152609 4 AGTACTT 11005 0.0 19.893608 12-13 TAAGGTG 745 0.0 19.12519 5 CATGGGA 7145 0.0 18.615427 4 CATGGGG 3530 0.0 18.435844 4 ATGGGAG 2760 0.0 18.412617 5 TAGCCCT 1165 0.0 18.348635 4 ACTTTTT 13290 0.0 18.188972 16-17 AGGTAAG 730 0.0 17.569962 2 GTGTAGC 875 0.0 17.556046 1 >>END_MODULE