FastQCFastQC Report
Thu 26 May 2016
SRR936385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719234
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT178271.036915277385161No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT120320.6998465595724608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95750.5569340764549794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77850.4528179410132652No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT73110.42524752302478896No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC70060.40750706419254157No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT50310.29263032257389043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48970.28483615377546045No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT43020.25022771769287955No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39970.23248725886063212No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37430.2177132374068917No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33830.19677367944096036No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC32540.1892703378365016No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30670.17839340078197616No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA27180.1580936626427816No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21150.12301990304984661No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA20620.11993713479375118No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC19770.1149930724962396No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.11441141810829708No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18790.10929285949440273No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC18020.10481412070724522No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT17960.10446512807447968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15000.025.9756972
GTACCTG15600.024.9759041
GTACATG109500.024.8206921
TACATGG111800.024.0132682
ACGTCGT500.001654047923.75065622-23
GAGTACT85700.023.20448112-13
GTACACG3500.023.078781
ACATGGG112500.022.8501643
AGTACTT87450.022.25109312-13
CGCGGAA1551.0913936E-1121.44939682-83
GTAAGCG1800.021.116394-95
GTACTTT93350.021.04836814-15
ATGGGAG30750.020.5518595
CATGGGA75500.020.2035624
TCGTACC1456.1333604E-419.6620069
CATGGGG35600.019.0878034
TACTTTT102450.018.85410114-15
ATTCGCG1655.984475E-1018.71345380-81
ACTTTTT107400.018.6916716-17
GTATAGG4400.018.358121
Produced by FastQC (version 0.11.5)