##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936385_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1719234 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.309910111130886 31.0 31.0 34.0 28.0 34.0 2 31.31920262163266 31.0 31.0 34.0 28.0 34.0 3 31.369641363537482 31.0 31.0 34.0 28.0 34.0 4 34.9944039031336 37.0 35.0 37.0 32.0 37.0 5 34.98659984621058 37.0 35.0 37.0 32.0 37.0 6 35.12618235795709 37.0 35.0 37.0 32.0 37.0 7 34.99955038115812 37.0 35.0 37.0 32.0 37.0 8 34.94802220058468 37.0 35.0 37.0 32.0 37.0 9 36.399458130772196 39.0 37.0 39.0 32.0 39.0 10-11 36.30404412662848 39.0 36.0 39.0 32.0 39.0 12-13 36.19384126884415 39.0 35.0 39.0 32.0 39.0 14-15 37.16378951323671 39.0 36.5 41.0 31.0 41.0 16-17 37.068596246933225 39.0 36.0 41.0 31.0 41.0 18-19 37.074867353716826 39.0 36.0 41.0 31.0 41.0 20-21 36.99561519839649 39.0 36.0 41.0 31.0 41.0 22-23 36.85284929218477 39.0 36.0 41.0 30.5 41.0 24-25 36.96651735598528 39.0 36.0 41.0 31.0 41.0 26-27 36.923654080829024 39.0 36.0 41.0 30.5 41.0 28-29 36.835612836879676 39.0 36.0 41.0 30.0 41.0 30-31 36.63736088281176 39.0 36.0 41.0 30.0 41.0 32-33 36.40281776651695 39.0 35.0 41.0 30.0 41.0 34-35 36.12678553355739 39.0 35.0 40.0 30.0 41.0 36-37 35.897493593076916 38.0 35.0 40.0 29.0 41.0 38-39 35.67392106019309 38.0 35.0 40.0 27.5 41.0 40-41 35.50094402507163 38.0 35.0 40.0 27.0 41.0 42-43 35.2288271986245 38.0 34.5 40.0 26.5 41.0 44-45 34.90551140798751 38.0 34.0 40.0 25.5 41.0 46-47 34.516662071597 38.0 33.5 40.0 24.0 41.0 48-49 34.313670506748934 38.0 33.0 40.0 23.0 41.0 50-51 33.359476371453795 37.5 32.0 39.5 21.0 40.5 52-53 33.50801403415707 37.5 33.0 39.5 22.0 40.5 54-55 33.922739720131176 38.0 33.0 40.0 22.0 41.0 56-57 33.794388082134255 38.0 33.0 40.0 20.5 41.0 58-59 33.530002314984465 38.0 33.0 40.0 19.5 41.0 60-61 33.15590140725463 37.0 32.5 40.0 18.0 41.0 62-63 32.860329949268106 37.0 32.0 40.0 16.0 41.0 64-65 32.52803050660934 36.0 31.5 39.0 13.5 41.0 66-67 32.09181821671744 36.0 31.0 39.0 10.0 41.0 68-69 31.665407675743964 35.0 31.0 39.0 8.0 40.0 70-71 31.18780020637098 35.0 30.5 38.0 7.0 40.0 72-73 30.787332323581317 35.0 30.0 37.0 4.0 39.5 74-75 30.373188291995156 35.0 30.0 37.0 2.0 39.0 76-77 29.939162150120346 34.0 29.0 36.0 2.0 39.0 78-79 29.468548493107978 34.0 29.0 36.0 2.0 38.5 80-81 28.950808615930118 34.0 28.5 35.0 2.0 37.0 82-83 28.450522732798444 34.0 27.5 35.0 2.0 37.0 84-85 28.120577245447684 34.0 27.0 35.0 2.0 36.0 86-87 27.839066118980895 34.0 26.5 35.0 2.0 36.0 88-89 27.398065940994652 33.5 26.0 35.0 2.0 35.5 90-91 26.948322043421662 33.0 25.0 35.0 2.0 35.0 92-93 26.553208289273016 33.0 25.0 35.0 2.0 35.0 94-95 26.06872392007138 33.0 24.0 35.0 2.0 35.0 96-97 25.471132783553607 32.5 20.5 35.0 2.0 35.0 98-99 24.77555905711497 32.0 10.0 35.0 2.0 35.0 100-101 22.32827497594859 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38689189280180614 1101 2 0.43020777084828765 1101 3 0.46352407730434564 1101 4 0.5186473884491178 1101 5 0.4187733129821041 1101 6 0.302132591188105 1101 7 0.3772602995319616 1101 8 0.4304041693003384 1101 9 0.7288192488619387 1101 10-11 0.6319254063869977 1101 12-13 0.6867070972713023 1101 14-15 0.8602497582273472 1101 16-17 0.8693056262479928 1101 18-19 0.8237187600402436 1101 20-21 0.75443751282166 1101 22-23 0.8049821463348152 1101 24-25 0.8398489201571593 1101 26-27 0.7905884764227409 1101 28-29 0.7213340608409666 1101 30-31 0.6605637067229182 1101 32-33 0.6998260776685541 1101 34-35 0.7625412586213614 1101 36-37 0.7471407612074117 1101 38-39 0.8562375201749788 1101 40-41 0.6963466710080297 1101 42-43 0.8927640842307341 1101 44-45 0.9116857358664916 1101 46-47 0.9307058058064044 1101 48-49 1.1773129166305765 1101 50-51 1.1782069448233585 1101 52-53 1.0466565055784685 1101 54-55 1.1889285687274764 1101 56-57 0.9548328256971104 1101 58-59 0.9476704980497672 1101 60-61 0.8391296873940561 1101 62-63 0.7679234640751176 1101 64-65 0.8088741712213405 1101 66-67 0.5940589027784284 1101 68-69 0.6927821872286977 1101 70-71 0.6954201694913031 1101 72-73 0.733355061813274 1101 74-75 0.5902734797860703 1101 76-77 0.6381911920604146 1101 78-79 0.6008248139163221 1101 80-81 0.7589519085905359 1101 82-83 0.8068175576972081 1101 84-85 0.9063990556132673 1101 86-87 0.7940616036424544 1101 88-89 0.871196607474829 1101 90-91 0.5493164089800899 1101 92-93 0.7114488130180874 1101 94-95 0.8846891856445218 1101 96-97 0.8822454088379743 1101 98-99 1.0385191685668573 1101 100-101 1.4075180058991812 1102 1 0.27404278034297036 1102 2 0.33095505722306484 1102 3 0.21133488302097447 1102 4 0.3198220915857277 1102 5 0.4301713704810268 1102 6 0.30874341464447497 1102 7 0.27016856292269154 1102 8 0.24275740070410734 1102 9 0.3918526882641089 1102 10-11 0.45484680826962176 1102 12-13 0.331889511649365 1102 14-15 0.45167758522057255 1102 16-17 0.4016949192086443 1102 18-19 0.5056942182243631 1102 20-21 0.4670270430512815 1102 22-23 0.32041765685115564 1102 24-25 0.18148116988829344 1102 26-27 0.24727441657331184 1102 28-29 0.3118765641394461 1102 30-31 0.18822605302473505 1102 32-33 0.22574632646834658 1102 34-35 0.20595676758546944 1102 36-37 0.381720080001557 1102 38-39 0.29196216066814173 1102 40-41 0.40553058771997286 1102 42-43 0.4080374424736135 1102 44-45 0.24255440084048274 1102 46-47 0.23828607811135072 1102 48-49 0.4508182052893446 1102 50-51 0.42861509734073877 1102 52-53 0.573256685193293 1102 54-55 0.5546804840645834 1102 56-57 0.8168627226957668 1102 58-59 0.864582287213004 1102 60-61 0.6190807368103108 1102 62-63 0.6151783785363278 1102 64-65 0.47388018610053706 1102 66-67 0.5429953501745644 1102 68-69 0.41622663413699357 1102 70-71 0.503638240738514 1102 72-73 0.30103381737079715 1102 74-75 0.3015889366397069 1102 76-77 0.540044162911812 1102 78-79 0.5232614220788179 1102 80-81 0.6353640192864844 1102 82-83 0.792144994416244 1102 84-85 0.8262700544712693 1102 86-87 0.696782851411129 1102 88-89 0.6662138205623158 1102 90-91 0.6689412690024845 1102 92-93 0.6251578868929997 1102 94-95 0.7163181646118453 1102 96-97 0.6197390336203839 1102 98-99 0.7390380558419238 1102 100-101 0.7529795439342841 1103 1 0.38406618218584754 1103 2 0.5012805356888528 1103 3 0.4116393394923179 1103 4 0.5297296467838493 1103 5 0.4575891391953206 1103 6 0.3251375613115215 1103 7 0.37357598613496634 1103 8 0.4058620907835575 1103 9 0.654776974890865 1103 10-11 0.6292085917335015 1103 12-13 0.6560270559466872 1103 14-15 0.560118524631541 1103 16-17 0.7547673172068343 1103 18-19 0.6732210496476654 1103 20-21 0.8437636143462086 1103 22-23 0.7262489693649172 1103 24-25 0.9190979568467199 1103 26-27 0.6565082284750119 1103 28-29 0.4165258852671698 1103 30-31 0.5406915896737203 1103 32-33 0.6674016067912447 1103 34-35 0.4633133030732566 1103 36-37 0.4764219082448875 1103 38-39 0.5615392178351115 1103 40-41 0.3117191510437678 1103 42-43 0.27114404737363884 1103 44-45 0.4774428420260861 1103 46-47 0.5533205653976268 1103 48-49 0.3112710056460557 1103 50-51 0.4838241615542458 1103 52-53 0.4796687354327176 1103 54-55 0.5020512302564839 1103 56-57 0.4755056072908985 1103 58-59 0.43576314338398703 1103 60-61 0.3438359475157995 1103 62-63 0.49304701910945425 1103 64-65 0.5868695082403761 1103 66-67 0.4806828487213579 1103 68-69 0.5730399076732162 1103 70-71 0.8226462542626187 1103 72-73 0.8550979556536689 1103 74-75 0.645901167282581 1103 76-77 0.6297908458270989 1103 78-79 0.7184087196515101 1103 80-81 0.6658214288898598 1103 82-83 0.5626232482092988 1103 84-85 0.6142772596675456 1103 86-87 0.7864507531370641 1103 88-89 0.8763712598454099 1103 90-91 0.8080350664883262 1103 92-93 0.8059530571684199 1103 94-95 0.8689600015265775 1103 96-97 1.0153937586453132 1103 98-99 1.2147072514394743 1103 100-101 1.5869583085741148 1104 1 0.25322327876120454 1104 2 0.3061867869005752 1104 3 0.25982045388816744 1104 4 0.281526069581993 1104 5 0.4279096219317182 1104 6 0.27133138591177186 1104 7 0.41158439096821553 1104 8 0.285860700228902 1104 9 0.4269915535193718 1104 10-11 0.5748888470607341 1104 12-13 0.4014911067661089 1104 14-15 0.48844639602663165 1104 16-17 0.35528168872293264 1104 18-19 0.6014987808507755 1104 20-21 0.6270569023795503 1104 22-23 0.4003688752885495 1104 24-25 0.5211930146282739 1104 26-27 0.11024006268345943 1104 28-29 0.3568257637273362 1104 30-31 0.43152489292292984 1104 32-33 0.2179759459769386 1104 34-35 0.20269554916666266 1104 36-37 0.22725871462218095 1104 38-39 0.4292538813676785 1104 40-41 0.28320596149914223 1104 42-43 0.28378900266864804 1104 44-45 0.19509245701626554 1104 46-47 0.3468592996987354 1104 48-49 0.35023768982835435 1104 50-51 0.23042632787795725 1104 52-53 0.3224940750823748 1104 54-55 0.36333284030484947 1104 56-57 0.3118613913965902 1104 58-59 0.3327294612086291 1104 60-61 0.4346697855937336 1104 62-63 0.6839888925877062 1104 64-65 0.5267597079053061 1104 66-67 0.6277429621317836 1104 68-69 0.6083491656919655 1104 70-71 0.7476433461314862 1104 72-73 0.6644478312391406 1104 74-75 0.39986230930233546 1104 76-77 0.5714622925253998 1104 78-79 0.562878390183652 1104 80-81 0.6388230079716379 1104 82-83 0.697265173699634 1104 84-85 0.7542678734236077 1104 86-87 0.7935748053203042 1104 88-89 0.9116691527539835 1104 90-91 0.913397927714982 1104 92-93 0.9424837302637279 1104 94-95 0.9423046732286586 1104 96-97 0.9604064140297659 1104 98-99 1.271692448717733 1104 100-101 1.7151329515425218 1105 1 0.20433847628234858 1105 2 0.22674568279401086 1105 3 0.17228674931172705 1105 4 0.25940315203249753 1105 5 0.34580420961452774 1105 6 0.18418587831526168 1105 7 0.25588103380516714 1105 8 0.32072084290543046 1105 9 0.28542303836878347 1105 10-11 0.315670919849417 1105 12-13 0.14281708635734702 1105 14-15 -0.014393287780059438 1105 16-17 0.2251776460056405 1105 18-19 0.37813061575094764 1105 20-21 0.4939262163107614 1105 22-23 0.25786731347591996 1105 24-25 0.3011638554047451 1105 26-27 0.4456778133715602 1105 28-29 0.2614873063375498 1105 30-31 0.25295037593992475 1105 32-33 0.14970540527861687 1105 34-35 0.039393256326057724 1105 36-37 0.1825206963987398 1105 38-39 0.09634533772057097 1105 40-41 -0.03414237565196032 1105 42-43 0.2760629129490795 1105 44-45 0.2507114399294039 1105 46-47 0.34340580130580634 1105 48-49 0.4189965074358497 1105 50-51 0.34339380419080356 1105 52-53 0.36803717774336775 1105 54-55 0.37460779394884725 1105 56-57 0.3297814907060541 1105 58-59 0.299907015934032 1105 60-61 0.2213109603893244 1105 62-63 0.41194174214480483 1105 64-65 0.3691680324002746 1105 66-67 0.5668395434391158 1105 68-69 0.48785993512747794 1105 70-71 0.45097855355016847 1105 72-73 0.5146691250692292 1105 74-75 0.515935630848773 1105 76-77 0.2473074426089248 1105 78-79 0.4640394251205713 1105 80-81 0.3535251671388657 1105 82-83 0.3516490382827371 1105 84-85 0.465512493285555 1105 86-87 0.5847440918002462 1105 88-89 0.4183025797736235 1105 90-91 0.6501403004255693 1105 92-93 0.5383411754965692 1105 94-95 0.4577594119122814 1105 96-97 0.7791491353363931 1105 98-99 0.7828242193673987 1105 100-101 1.3209113753873503 1106 1 0.16579011256038356 1106 2 0.14144907156414632 1106 3 0.21332879289722584 1106 4 0.30208583236985476 1106 5 0.28638846439667276 1106 6 0.2107223390333388 1106 7 0.42105020006415117 1106 8 0.36054739630601773 1106 9 0.6399961001913113 1106 10-11 0.34446731231854955 1106 12-13 0.5922368822924895 1106 14-15 0.7825846516230754 1106 16-17 0.7261354632712553 1106 18-19 0.7625638989521804 1106 20-21 0.43628145679219443 1106 22-23 0.5952696514520426 1106 24-25 0.3573358643157718 1106 26-27 0.09571206434497981 1106 28-29 0.5326837914259315 1106 30-31 0.4962719861179252 1106 32-33 0.2999082298908107 1106 34-35 0.24588482640663045 1106 36-37 0.2824099272932017 1106 38-39 0.39246817152869085 1106 40-41 0.3649631390710084 1106 42-43 0.09940983942264126 1106 44-45 0.4604115466010441 1106 46-47 0.47338886419257165 1106 48-49 0.47296922160618493 1106 50-51 0.21200069019019452 1106 52-53 0.34044115058129165 1106 54-55 0.3873465160836673 1106 56-57 0.11751427976378181 1106 58-59 0.2957018873904431 1106 60-61 0.3762074471001924 1106 62-63 0.45250996455641257 1106 64-65 0.21312769242601348 1106 66-67 0.32171986028055244 1106 68-69 0.5730902718925144 1106 70-71 0.6392209959924493 1106 72-73 0.6708812011567211 1106 74-75 0.3971739898490263 1106 76-77 0.6394472635798714 1106 78-79 0.8366102666110251 1106 80-81 0.8754618774999265 1106 82-83 0.9674275033716313 1106 84-85 0.7520328171339408 1106 86-87 0.8491017946117978 1106 88-89 1.0605233188411525 1106 90-91 1.315888199511285 1106 92-93 1.2568843857601486 1106 94-95 1.4284446486290001 1106 96-97 1.3949827374800563 1106 98-99 1.681627786528594 1106 100-101 1.9070573172588716 1107 1 0.09572858802148332 1107 2 0.10309530884221019 1107 3 -0.030352684231989002 1107 4 0.007790798230828955 1107 5 0.1583574522470954 1107 6 0.1917346912157356 1107 7 0.23106107322634983 1107 8 0.2912068772307066 1107 9 0.2925122579804764 1107 10-11 0.3004885453756643 1107 12-13 0.3171996366468264 1107 14-15 0.25622378420137437 1107 16-17 0.3339540097793545 1107 18-19 0.3454036177357409 1107 20-21 0.4239435918490244 1107 22-23 0.5592802949946716 1107 24-25 0.32485928213559845 1107 26-27 0.18562163475377247 1107 28-29 0.29001601718249503 1107 30-31 0.17436033562592712 1107 32-33 0.1927177604538528 1107 34-35 0.3689035133878704 1107 36-37 0.4207018278003929 1107 38-39 0.344122985773204 1107 40-41 0.2788984809002599 1107 42-43 0.4642783146811311 1107 44-45 0.5067928096437768 1107 46-47 0.4045998373068258 1107 48-49 0.4921920268820301 1107 50-51 0.44701808049619984 1107 52-53 0.4320585503422123 1107 54-55 0.5797844681472029 1107 56-57 0.36672215976729206 1107 58-59 0.542667734855506 1107 60-61 0.5154090463329908 1107 62-63 0.5335071888609662 1107 64-65 0.5393915793451285 1107 66-67 0.5161022849281949 1107 68-69 0.5863425630231127 1107 70-71 0.8344634134813553 1107 72-73 0.8148388971268936 1107 74-75 0.6711811890980357 1107 76-77 0.5663964973933808 1107 78-79 0.7020171322362998 1107 80-81 0.7525392255289134 1107 82-83 0.9350553305626406 1107 84-85 0.9565883344195676 1107 86-87 0.7858031068201292 1107 88-89 0.7343362869442949 1107 90-91 0.6660157734454089 1107 92-93 0.7838410484422376 1107 94-95 0.9627487324806623 1107 96-97 0.8629548589623148 1107 98-99 1.315739395148782 1107 100-101 1.478791147651048 1108 1 0.0525004677059826 1108 2 0.23835806119233993 1108 3 0.22492323725752428 1108 4 0.24106585111753276 1108 5 0.24370216147529788 1108 6 0.04136805677143229 1108 7 0.10991367693332421 1108 8 0.13809713907242127 1108 9 0.35346463893285573 1108 10-11 0.007892263556080081 1108 12-13 0.2809699332326545 1108 14-15 0.34903077225107637 1108 16-17 0.4774843198145149 1108 18-19 0.25388384301208333 1108 20-21 0.2760271178160494 1108 22-23 0.4099369306998497 1108 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84-85 -2.558055702048044 2316 86-87 -3.3047584443048024 2316 88-89 -3.4853028878171948 2316 90-91 -3.4211254470835044 2316 92-93 -3.73042637427589 2316 94-95 -4.361139284617138 2316 96-97 -5.525623501150513 2316 98-99 -6.843806588889414 2316 100-101 -6.820998980678741 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4774.0 3 1925.0 4 1891.0 5 2017.0 6 2179.0 7 2563.0 8 3317.0 9 4026.0 10 5228.0 11 6714.0 12 8475.0 13 10507.0 14 11809.0 15 14639.0 16 17618.0 17 21940.0 18 18500.0 19 12770.0 20 12936.0 21 13578.0 22 14958.0 23 16849.0 24 18736.0 25 21829.0 26 25448.0 27 29260.0 28 34667.0 29 41663.0 30 50375.0 31 61566.0 32 76282.0 33 96423.0 34 125759.0 35 167958.0 36 225670.0 37 283045.0 38 218009.0 39 33323.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.3537687682009 18.305545292421733 14.11694541984622 21.223740519531148 2 15.26170773254894 21.15932383248471 39.529247223747774 24.04972121121857 3 21.08240942154405 25.555408818240366 29.60161594629957 23.76056581391601 4 12.330300152241016 16.504415920592578 38.582273000265374 32.58301092690103 5 12.8845592009619 38.57330587929925 33.56214928305629 14.979985636682564 6 29.79364662763339 36.5180698141196 17.834590246647537 15.853693311599471 7 27.71243146105079 33.856174994151424 20.78195215687461 17.64944138792318 8 26.2336454689133 32.40053442452766 19.82677755510089 21.539042551458145 9 26.4860722280769 17.6835481267381 21.423147177756068 34.40723246742893 10-11 25.167319377855037 26.340482749249205 26.669811164285644 21.822386708610114 12-13 27.636228206978426 24.191330509066365 26.93774586252648 21.234695421428736 14-15 23.513108387575805 25.32862617674066 27.66537795938128 23.492887476302258 16-17 21.24863716552543 28.79915478802632 27.874565980683613 22.07764206576464 18-19 20.964546766609246 28.001390893430795 30.11403580241166 20.920026537548303 20-21 22.756141956223164 25.67472369704666 29.717888562026296 21.851245784703877 22-23 21.73107404980093 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28.797531674210774 21.477389191157396 48-49 22.50281670560904 26.875443154896416 28.633230320723452 21.988509818771096 50-51 21.365607121335856 27.407708576174887 29.493266745306613 21.733417557182644 52-53 22.215701010251422 27.940652963978092 27.928001554232956 21.915644471537526 54-55 21.484534967052603 26.959974498343325 28.515523202784703 23.039967331819366 56-57 22.4940944864712 26.965646651186237 27.92274561511439 22.617513247228167 58-59 23.50671622125515 26.667479132333206 27.728473974242707 22.09733067216894 60-61 22.331566069938955 27.015163096654998 29.33212219364041 21.321148639765635 62-63 21.883944373106782 27.28483697446241 28.9699601397798 21.861258512651002 64-65 21.476960947120023 28.389403879520692 28.238231816164532 21.89540335719475 66-67 22.019379660082297 27.371064997132255 27.57872914196898 23.030826200816463 68-69 21.60644490771485 27.443070243641642 28.202201533097316 22.748283315546196 70-71 22.609017605457655 27.301460160569963 27.64968943521301 22.43983279875937 72-73 22.303830468901324 26.68726358765674 28.90234872372962 22.106557219712318 74-75 21.435283544844978 28.67804132511426 27.81140345136977 22.075271678670987 76-77 21.89482023833657 27.325640071815666 27.76089094239012 23.018648747457643 78-79 22.134598068345483 27.464214831998174 28.075913184903218 22.325273914753122 80-81 22.606481839668326 27.499041621268503 27.662038720245903 22.232437818817264 82-83 22.183433064417144 27.295138276900488 28.46428091532718 22.057147743355188 84-85 21.895157912373293 27.896892714529052 27.940233680526465 22.267715692571183 86-87 21.865515868410895 28.098754358610584 27.617849355598928 22.41788041737959 88-89 21.666666666666668 27.916666666666668 26.984590953511102 23.432075713155566 90-91 22.04036724783994 27.170092940414133 27.367019822402256 23.42251998934367 92-93 22.689006700032056 27.699806916676312 27.610071747412807 22.001114635878828 94-95 21.977322629988542 27.36557580700472 27.9000934540567 22.757008108950036 96-97 21.00951007564258 27.48655306076565 28.709626097576514 22.794310766015258 98-99 21.675977653631286 28.044983630019534 27.168902418626434 23.110136297722747 100-101 21.34660983663791 27.279199336659733 27.676214305456554 23.6979765212458 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 376.0 1 326.5 2 529.5 3 768.5 4 641.5 5 568.5 6 889.0 7 1753.5 8 2802.0 9 3318.0 10 2920.5 11 2401.5 12 2407.0 13 2538.0 14 2653.0 15 2807.0 16 2931.0 17 3135.5 18 3323.0 19 3529.5 20 3930.0 21 4471.0 22 5192.5 23 6080.5 24 7103.0 25 8526.5 26 10341.0 27 12660.5 28 14866.5 29 17274.5 30 20464.0 31 23941.0 32 28133.0 33 31471.0 34 34233.0 35 36556.0 36 39792.5 37 43709.0 38 45093.0 39 45600.0 40 46904.0 41 49291.0 42 50751.5 43 52356.0 44 54871.5 45 57421.0 46 60087.0 47 72346.0 48 101505.5 49 99929.0 50 71000.0 51 57471.5 52 51476.5 53 49439.0 54 48138.0 55 45089.0 56 41391.5 57 37457.5 58 34359.0 59 30884.0 60 26211.0 61 22118.0 62 18795.5 63 14683.0 64 10712.0 65 8346.0 66 6388.5 67 4934.0 68 3650.0 69 2529.5 70 1877.0 71 1453.0 72 1211.5 73 1122.5 74 909.0 75 712.5 76 562.5 77 340.5 78 201.0 79 136.5 80 96.0 81 61.0 82 44.0 83 28.5 84 17.0 85 14.0 86 11.5 87 8.5 88 5.5 89 2.5 90 3.0 91 3.5 92 2.0 93 1.0 94 1.0 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03309613467393037 2 0.021521212353873876 3 0.02123038515990261 4 0.05269788754759387 5 0.056304144752837605 6 0.05066209718979499 7 0.050487600873412225 8 0.04362407909569029 9 0.04426389892242708 10-11 0.05167999236869443 12-13 0.04618335840263745 14-15 0.041646454176685666 16-17 0.049353374816924284 18-19 0.053163211057947905 20-21 0.011458591442467983 22-23 0.021550295073271004 24-25 0.006369115547970783 26-27 0.011313177845482348 28-29 0.005932874757013879 30-31 0.009422801084669103 32-33 0.0038389189604207457 34-35 0.007997747834209886 36-37 0.009655462839846118 38-39 0.011720335917042125 40-41 0.0018903767608132458 42-43 0.002093955796593134 44-45 0.0023266175517701487 46-47 6.979852655310447E-4 48-49 0.0015704668474448506 50-51 3.4899263276552233E-4 52-53 0.0033735954500667157 54-55 0.007561507043252983 56-57 0.01683889453093645 58-59 0.010876937054525446 60-61 0.011167764248496715 62-63 0.005903792037616753 64-65 5.8165438794253716E-5 66-67 0.0065145291449564165 68-69 0.017187887163701975 70-71 0.006281867389779402 72-73 0.0026174447457414174 74-75 0.0035771744858466036 76-77 0.006427280986765036 78-79 0.00500222773630582 80-81 0.010760606176936938 82-83 0.006456363706162163 84-85 0.01779862427104164 86-87 0.004624152384143171 88-89 0.006630860022544923 90-91 0.003955249838009253 92-93 0.01669348093395082 94-95 0.012069328549807646 96-97 0.009451883804066228 98-99 0.02248094209397906 100-101 0.0383310241654132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1719234.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.540169688853204 #Duplication Level Percentage of deduplicated Percentage of total 1 80.04809711335892 46.05981091172473 2 12.040002881386355 13.85567617698505 3 3.5239384724967113 6.083040530416168 4 1.4900340287903604 3.429472434350517 5 0.7634006582798425 2.1963101709002193 6 0.4671443153807032 1.6127737905713293 7 0.29796358331108996 1.2001412601373207 8 0.2185718375120091 1.0061328495716364 9 0.15364421808005418 0.7956642942033737 >10 0.8907311192137203 9.543857785643375 >50 0.0682052045594336 2.7049148868535062 >100 0.03281469069175899 3.5907665791197916 >500 0.002322095733296322 0.8612860607629034 >1k 0.0024230564173526835 3.1930789376657884 >5k 5.048034202818091E-4 2.1324759691410127 >10k+ 2.0192136811272363E-4 1.7345973619532935 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17827 1.036915277385161 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12032 0.6998465595724608 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9575 0.5569340764549794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7785 0.4528179410132652 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7311 0.42524752302478896 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7006 0.40750706419254157 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5031 0.29263032257389043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4897 0.28483615377546045 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4302 0.25022771769287955 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3997 0.23248725886063212 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3743 0.2177132374068917 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3383 0.19677367944096036 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3254 0.1892703378365016 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3067 0.17839340078197616 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2718 0.1580936626427816 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2115 0.12301990304984661 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2062 0.11993713479375118 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1977 0.1149930724962396 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1967 0.11441141810829708 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1879 0.10929285949440273 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1802 0.10481412070724522 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1796 0.10446512807447968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.816543879425372E-5 2 0.0 0.0 0.0 0.0 5.816543879425372E-5 3 0.0 0.0 0.0 0.0 5.816543879425372E-5 4 0.0 0.0 0.0 0.0 1.1633087758850744E-4 5 0.0 0.0 0.0 0.0 1.1633087758850744E-4 6 0.0 0.0 0.0 0.0 3.4899263276552233E-4 7 0.0 0.0 0.0 0.0 3.4899263276552233E-4 8 0.0 0.0 0.0 0.0 3.4899263276552233E-4 9 0.0 0.0 0.0 0.0 3.4899263276552233E-4 10-11 0.0 0.0 0.0 0.0 3.4899263276552233E-4 12-13 0.0 0.0 0.0 0.0 3.4899263276552233E-4 14-15 0.0 0.0 0.0 0.0 3.4899263276552233E-4 16-17 0.0 0.0 0.0 0.0 4.0715807155977603E-4 18-19 0.0 0.0 0.0 0.0 4.0715807155977603E-4 20-21 0.0 0.0 0.0 0.0 4.0715807155977603E-4 22-23 0.0 0.0 0.0 5.816543879425372E-5 4.362407909569029E-4 24-25 0.0 0.0 0.0 1.4541359698563432E-4 5.234889491482835E-4 26-27 0.0 0.0 0.0 5.525716685454104E-4 5.234889491482835E-4 28-29 0.0 0.0 0.0 8.724815819138058E-4 5.234889491482835E-4 30-31 0.0 0.0 0.0 0.0019194594802103726 5.234889491482835E-4 32-33 0.0 0.0 0.0 0.0030536855366983203 5.816543879425372E-4 34-35 0.0 0.0 0.0 0.0042460770319805215 5.816543879425372E-4 36-37 0.0 0.0 0.0 0.005525716685454103 5.816543879425372E-4 38-39 0.0 0.0 0.0 0.00860848494154955 5.816543879425372E-4 40-41 0.0 0.0 0.0 0.013319885483884103 5.816543879425372E-4 42-43 0.0 0.0 0.0 0.017827706990438764 5.816543879425372E-4 44-45 0.0 0.0 0.0 0.01980533190944339 5.816543879425372E-4 46-47 0.0 0.0 0.0 0.02445856701298369 6.398198267367909E-4 48-49 0.0 0.0 0.0 0.030711351683365966 6.398198267367909E-4 50-51 0.0 0.0 0.0 0.038418272323604584 6.398198267367909E-4 52-53 0.0 0.0 0.0 0.05182540596568007 6.689025461339178E-4 54-55 0.0 0.0 0.0 0.062091605912865847 6.979852655310447E-4 56-57 0.0 0.0 0.0 0.08864412872244268 6.979852655310447E-4 58-59 0.0 0.0 0.0 0.1292726877202289 6.979852655310447E-4 60-61 0.0 0.0 0.0 0.16443369547135528 7.270679849281714E-4 62-63 0.0 0.0 0.0 0.18610032142221478 7.561507043252983E-4 64-65 0.0 0.0 0.0 0.2276013620019148 7.561507043252983E-4 66-67 0.0 0.0 0.0 0.2997846715455837 7.561507043252983E-4 68-69 0.0 0.0 0.0 0.40247575373683864 7.561507043252983E-4 70-71 0.0 0.0 0.0 0.4923413566739606 7.561507043252983E-4 72-73 0.0 0.0 0.0 0.5902047074452925 7.561507043252983E-4 74-75 0.0 0.0 0.0 0.7060120960846517 7.561507043252983E-4 76-77 0.0 0.0 0.0 0.790235651458731 7.561507043252983E-4 78-79 0.0 0.0 0.0 0.9009826469229901 7.561507043252983E-4 80-81 0.0 0.0 0.0 1.0469197328577726 7.561507043252983E-4 82-83 0.0 0.0 0.0 1.1802930840129964 7.561507043252983E-4 84-85 0.0 0.0 0.0 1.3504269924861887 7.561507043252983E-4 86-87 0.0 0.0 0.0 1.5938784365595375 7.561507043252983E-4 88-89 0.0 0.0 0.0 1.8922962202934563 7.561507043252983E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1500 0.0 25.975697 2 GTACCTG 1560 0.0 24.975904 1 GTACATG 10950 0.0 24.820692 1 TACATGG 11180 0.0 24.013268 2 ACGTCGT 50 0.0016540479 23.750656 22-23 GAGTACT 8570 0.0 23.204481 12-13 GTACACG 350 0.0 23.07878 1 ACATGGG 11250 0.0 22.850164 3 AGTACTT 8745 0.0 22.251093 12-13 CGCGGAA 155 1.0913936E-11 21.449396 82-83 GTAAGCG 180 0.0 21.1163 94-95 GTACTTT 9335 0.0 21.048368 14-15 ATGGGAG 3075 0.0 20.551859 5 CATGGGA 7550 0.0 20.203562 4 TCGTACC 145 6.1333604E-4 19.662006 9 CATGGGG 3560 0.0 19.087803 4 TACTTTT 10245 0.0 18.854101 14-15 ATTCGCG 165 5.984475E-10 18.713453 80-81 ACTTTTT 10740 0.0 18.69167 16-17 GTATAGG 440 0.0 18.35812 1 >>END_MODULE