FastQCFastQC Report
Thu 26 May 2016
SRR936385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719234
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT211671.2311878429579683No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109290.6356900805823988No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT99510.5788042814416188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95780.557108572771362No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC62740.36492996299514785No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61700.3588807573605455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58960.34294342713091996No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT55070.32031707143995525No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC50150.2916996755531824No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC47850.278321624630504No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT41220.23975793870991383No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA39190.22795035463468033No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG39110.2274850311243263No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA33540.19508688171592697No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC31150.18118534184410034No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.1571048501832793No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC23320.13564180326819966No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21800.1268006565714731No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21500.1250556934076455No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19900.11574922320056491No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT18280.10632642211589581No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT17800.10353448105377162No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC17470.10161502157356125No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.10062620911405894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1601.906301E-929.68742
GTACACG2700.026.399551
GTACATG101650.024.1218911
GTACCTG15200.023.446971
TACATGG102750.023.3916472
ATCACGC1852.5432564E-723.1080283
TACCTGG14600.022.7738972
GAGTACT101100.022.17469812-13
ACATGGG102150.021.762043
AGTACTT103550.021.67297712-13
GTACTTT110500.020.2238714-15
GCGTATC1200.00475332319.7996621
CATGGGG37100.019.4597194
TGGACCG2001.2860217E-518.998835
CATGGGA67950.018.803114
CCGTCTA1559.6292625E-418.3865019
TACTTTT121950.018.18871314-15
ACTTTTT127350.017.7712616-17
AGAGTAC186250.017.5329910-11
ATGGGAT26350.017.1241845
Produced by FastQC (version 0.11.5)