##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936385_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1719234 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80477526619413 31.0 31.0 34.0 30.0 34.0 2 31.938719220303927 31.0 31.0 34.0 30.0 34.0 3 32.038947577816636 33.0 31.0 34.0 30.0 34.0 4 35.78836621425588 37.0 35.0 37.0 35.0 37.0 5 35.582572820221095 37.0 35.0 37.0 33.0 37.0 6 35.704487579933854 37.0 35.0 37.0 33.0 37.0 7 35.64837072789393 37.0 35.0 37.0 33.0 37.0 8 35.63116422778982 37.0 35.0 37.0 33.0 37.0 9 37.23813686793072 39.0 37.0 39.0 33.0 39.0 10-11 37.0954483217526 39.0 37.0 39.0 33.0 39.0 12-13 37.003401224033496 39.0 37.0 39.0 33.0 39.0 14-15 38.01045262017852 40.0 37.0 41.0 33.0 41.0 16-17 37.970682292230144 40.0 37.0 41.0 33.0 41.0 18-19 38.1857827381264 40.0 37.5 41.0 33.0 41.0 20-21 38.232050436415285 40.0 38.0 41.0 33.0 41.0 22-23 38.133149414215865 40.0 37.0 41.0 33.0 41.0 24-25 37.99155554159585 40.0 37.0 41.0 33.0 41.0 26-27 37.75184064531065 40.0 37.0 41.0 32.5 41.0 28-29 37.66000614227033 39.0 37.0 41.0 32.5 41.0 30-31 37.597926460272426 39.0 37.0 41.0 33.0 41.0 32-33 37.39333825412945 39.0 36.0 41.0 32.0 41.0 34-35 37.1637077907952 39.0 36.0 41.0 31.5 41.0 36-37 36.86062339390682 39.0 35.0 41.0 31.0 41.0 38-39 36.67215632078007 39.0 35.0 41.0 30.0 41.0 40-41 36.473807812083756 39.0 35.0 40.0 30.0 41.0 42-43 36.173422873209816 39.0 35.0 40.0 30.0 41.0 44-45 35.844800940418814 38.5 35.0 40.0 29.5 41.0 46-47 35.398173547056416 38.0 35.0 40.0 27.5 41.0 48-49 34.95636894105165 38.0 34.0 40.0 26.0 41.0 50-51 34.5986703962346 38.0 34.0 40.0 24.0 41.0 52-53 34.33103754346412 38.0 34.0 40.0 24.0 41.0 54-55 34.07032114302067 38.0 33.0 40.0 23.0 41.0 56-57 33.716841919133756 37.5 33.0 40.0 22.5 41.0 58-59 33.37670439277027 37.0 32.5 40.0 21.5 41.0 60-61 32.95759855842776 36.5 32.0 39.0 20.0 41.0 62-63 32.58746075287017 36.0 31.0 39.0 18.5 40.0 64-65 32.18235912039897 35.5 31.0 39.0 17.5 40.0 66-67 31.67506226610223 35.0 31.0 38.0 14.0 40.0 68-69 31.36885234936024 35.0 30.5 38.0 12.5 40.0 70-71 30.90319438773314 34.5 30.0 37.0 8.0 39.5 72-73 30.42315880211769 34.0 29.5 37.0 7.5 39.0 74-75 29.914214120939906 34.0 29.0 36.0 6.5 39.0 76-77 28.53282450207476 32.5 27.0 35.0 2.0 37.0 78-79 29.29259077007551 34.0 29.0 35.0 2.0 37.0 80-81 29.051030575244553 34.0 29.0 35.0 2.0 37.0 82-83 28.68783219736231 34.0 28.5 35.0 2.0 36.5 84-85 28.254509857296913 33.0 27.5 35.0 2.0 36.0 86-87 27.901121953148902 33.0 27.0 35.0 2.0 36.0 88-89 27.456207822786197 33.0 26.0 35.0 2.0 35.0 90-91 26.909734800498363 32.5 25.5 35.0 2.0 35.0 92-93 26.40415877070835 32.0 25.0 34.0 2.0 35.0 94-95 25.77206535003379 32.0 23.5 34.0 2.0 35.0 96-97 24.94765255922114 32.0 19.0 34.0 2.0 35.0 98-99 23.988661229361448 31.0 6.0 34.0 2.0 35.0 100-101 20.803809719910145 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23274236930378578 1101 2 0.2960752084092597 1101 3 0.2675665754185559 1101 4 0.17647604751532953 1101 5 0.17396131193032005 1101 6 0.1578777451013167 1101 7 0.2155322471899268 1101 8 0.19512903219123956 1101 9 0.3033322716193112 1101 10-11 0.25697860712751464 1101 12-13 0.3124116957808454 1101 14-15 0.3984573286341373 1101 16-17 0.4377451489368269 1101 18-19 0.3406424500058378 1101 20-21 0.2752453806560027 1101 22-23 0.28993906266557445 1101 24-25 0.26732831078142993 1101 26-27 0.33100053581014066 1101 28-29 0.18962784235094432 1101 30-31 0.28100744051124593 1101 32-33 0.5088701636804771 1101 34-35 0.43818725877624587 1101 36-37 0.4102255769273242 1101 38-39 0.26240208964723877 1101 40-41 0.38390366884139837 1101 42-43 0.37555036548017284 1101 44-45 0.40254761557137186 1101 46-47 0.3662987798248807 1101 48-49 0.3347499177074482 1101 50-51 0.23005344358447388 1101 52-53 0.3614332959013282 1101 54-55 0.2571133842549642 1101 56-57 0.2593926610541075 1101 58-59 0.2896581099382587 1101 60-61 0.08221667527401166 1101 62-63 0.3488892656258926 1101 64-65 0.312791936912042 1101 66-67 0.2525584377115955 1101 68-69 0.1735825229381831 1101 70-71 0.17690928102651426 1101 72-73 0.20245152956101364 1101 74-75 0.17030009876894425 1101 76-77 -0.02835724934976014 1101 78-79 -0.029067012982281426 1101 80-81 0.25399125444647197 1101 82-83 0.3810733939078226 1101 84-85 0.3871127896665385 1101 86-87 0.2951666669824604 1101 88-89 0.24872683162401543 1101 90-91 0.3143587617818504 1101 92-93 0.30274518925230254 1101 94-95 0.22707546978541515 1101 96-97 0.4058747444604762 1101 98-99 0.4273400884394647 1101 100-101 0.7040465733968375 1102 1 0.236794513039996 1102 2 0.2430397732867995 1102 3 0.1895788158363274 1102 4 0.11538344609392936 1102 5 0.05497591395042889 1102 6 0.0973406191602777 1102 7 0.17365760059873026 1102 8 0.1508520373024993 1102 9 0.22364981289191377 1102 10-11 0.1620744848563831 1102 12-13 0.09998972290046027 1102 14-15 0.29652813235504993 1102 16-17 0.2064576598855723 1102 18-19 0.20948301967310812 1102 20-21 0.06412118033003367 1102 22-23 0.06281071013267336 1102 24-25 0.029362365530687384 1102 26-27 -0.16269742301150103 1102 28-29 -0.13678842609561315 1102 30-31 -0.006258893904949048 1102 32-33 -0.055258427230121754 1102 34-35 0.027644450576055135 1102 36-37 0.2623783215970903 1102 38-39 0.1320262567112067 1102 40-41 0.11741444573095805 1102 42-43 0.18773756404325326 1102 44-45 0.1953019035981569 1102 46-47 0.0755898279106475 1102 48-49 0.14960043271415913 1102 50-51 -0.05158468243417502 1102 52-53 -0.0813908423355656 1102 54-55 0.04314639125107078 1102 56-57 0.0759290053380326 1102 58-59 -0.022940705934367145 1102 60-61 -0.13500943983470393 1102 62-63 -0.18576877324630914 1102 64-65 -0.1524119868957925 1102 66-67 -0.24051502696969962 1102 68-69 -0.25520671236832726 1102 70-71 -0.13824876893301408 1102 72-73 -0.12196846423008267 1102 74-75 -0.3035613705246796 1102 76-77 -0.5802343351546462 1102 78-79 -0.3743147040447852 1102 80-81 0.004310153917604254 1102 82-83 -0.16335023098546486 1102 84-85 0.034631028332512415 1102 86-87 0.04531565304568019 1102 88-89 0.046421774690553974 1102 90-91 0.15581250542048863 1102 92-93 0.00614290304384113 1102 94-95 -0.017987617150378554 1102 96-97 0.3000046935696119 1102 98-99 0.4508597906336469 1102 100-101 0.9265603771288333 1103 1 0.1719630651210622 1103 2 0.16871300368484654 1103 3 0.15078263617692045 1103 4 0.11670193683235652 1103 5 0.040731290151128974 1103 6 0.14671590894786135 1103 7 0.1927590300122901 1103 8 0.14762665881769976 1103 9 0.1965583843126666 1103 10-11 0.16778653140293898 1103 12-13 0.16597594439870278 1103 14-15 0.2267088503854069 1103 16-17 0.38192689350599096 1103 18-19 0.1658679204922393 1103 20-21 0.22890735641966842 1103 22-23 0.24706965405441395 1103 24-25 0.14439384381320508 1103 26-27 0.16824853938416595 1103 28-29 0.17846530814644979 1103 30-31 0.053541226181657464 1103 32-33 0.17457351990989167 1103 34-35 0.13592208314661036 1103 36-37 0.24894995845409795 1103 38-39 0.4577412028431951 1103 40-41 0.33547079342955044 1103 42-43 0.3172379736780826 1103 44-45 0.27117789981775076 1103 46-47 0.47109369242889443 1103 48-49 0.29339388375424136 1103 50-51 0.29486818083805844 1103 52-53 0.3218614804101776 1103 54-55 0.1967584951054704 1103 56-57 0.17052168880861984 1103 58-59 0.2753243298522605 1103 60-61 0.17088451466642596 1103 62-63 0.2740225933748164 1103 64-65 0.0753310277703676 1103 66-67 0.25627067315025087 1103 68-69 0.3525425664965667 1103 70-71 0.20814008462287248 1103 72-73 0.2960776255011446 1103 74-75 0.31253707313651446 1103 76-77 0.2724664508792003 1103 78-79 0.21800536094969658 1103 80-81 0.40708431861132155 1103 82-83 0.4922757170216876 1103 84-85 0.2263844432098452 1103 86-87 0.1272805051463095 1103 88-89 0.01592401708221658 1103 90-91 0.21533114688264732 1103 92-93 0.2813373599118201 1103 94-95 0.27971829102850165 1103 96-97 0.32103571503064643 1103 98-99 0.4728997857645325 1103 100-101 0.8087521354675324 1104 1 0.18811435746779637 1104 2 0.2845240112620324 1104 3 0.15864568793630696 1104 4 0.07854057098678169 1104 5 0.028048629431758343 1104 6 0.049577108862820296 1104 7 0.047426547056609536 1104 8 -0.0010164138802295497 1104 9 0.10083500842033999 1104 10-11 0.06980377160655138 1104 12-13 0.054761388500402575 1104 14-15 0.10266495699281819 1104 16-17 0.13863758994657616 1104 18-19 0.026083616912764285 1104 20-21 0.04129251866665129 1104 22-23 0.0883334275651535 1104 24-25 0.06658896804722758 1104 26-27 0.11698052735587083 1104 28-29 -0.011563479009105038 1104 30-31 -0.033201840766864166 1104 32-33 -0.09301091283072083 1104 34-35 -0.14486232338739313 1104 36-37 0.06703465660346097 1104 38-39 0.1325201514501657 1104 40-41 -0.06537731120433676 1104 42-43 -0.08137401210081663 1104 44-45 -0.018216981604034288 1104 46-47 0.034503519639102365 1104 48-49 -0.027124953581612488 1104 50-51 -0.22401314468142886 1104 52-53 -0.23038326255782948 1104 54-55 -0.17360517504961592 1104 56-57 -0.0198703807512004 1104 58-59 -0.34015107270662526 1104 60-61 -0.1621192304716459 1104 62-63 -0.07797866882095406 1104 64-65 -0.008005447681661337 1104 66-67 -0.2272182284853912 1104 68-69 -0.07481753233977884 1104 70-71 -0.14411856389170552 1104 72-73 -0.09095264446701634 1104 74-75 -0.07080434948112568 1104 76-77 0.07388909525955256 1104 78-79 0.05981425862760403 1104 80-81 0.18626450641642478 1104 82-83 -0.05500902444879685 1104 84-85 0.043117629197869434 1104 86-87 -0.09693359748464658 1104 88-89 -0.08608961302756768 1104 90-91 0.00440967711985607 1104 92-93 3.711108276505115E-5 1104 94-95 0.1028293094359718 1104 96-97 0.4570958725644445 1104 98-99 0.8478326313340787 1104 100-101 1.240827417957373 1105 1 0.015214493700419496 1105 2 0.18741576895923018 1105 3 0.0934320974321281 1105 4 0.07467665059598971 1105 5 -0.036653920356997105 1105 6 0.06937650181002653 1105 7 0.028642500686331118 1105 8 0.008446594665223017 1105 9 0.04796586636260258 1105 10-11 -0.018378768796516454 1105 12-13 -0.0809158805324941 1105 14-15 -0.007814260190869504 1105 16-17 0.06475368320329267 1105 18-19 -0.005838782484005378 1105 20-21 -0.027187475988569076 1105 22-23 -0.05695313104033772 1105 24-25 -0.08022519012431673 1105 26-27 0.049044574200273416 1105 28-29 0.008963412858498998 1105 30-31 0.12374557861221547 1105 32-33 0.09368907070929566 1105 34-35 -0.009088611246362177 1105 36-37 -0.15997073370422044 1105 38-39 0.023339818193427675 1105 40-41 -0.03710978233269202 1105 42-43 -0.10316853817319327 1105 44-45 -0.09841171586420927 1105 46-47 0.07948709063644799 1105 48-49 -0.1119791291775698 1105 50-51 -0.13250197007710796 1105 52-53 0.12875794119763384 1105 54-55 0.11943518854629076 1105 56-57 0.06344784405983717 1105 58-59 0.044425653847262936 1105 60-61 0.03630519086297568 1105 62-63 0.058443340358387275 1105 64-65 -0.3651560980126263 1105 66-67 0.006842729485619259 1105 68-69 0.1361337172854249 1105 70-71 0.13818778952702715 1105 72-73 0.20259822105599667 1105 74-75 -0.08260152369805951 1105 76-77 -0.17579753198756265 1105 78-79 0.04093619369660928 1105 80-81 0.295198344019699 1105 82-83 0.21366450296865835 1105 84-85 0.14863447131646268 1105 86-87 0.11024748091632475 1105 88-89 0.2705378738509161 1105 90-91 0.3164502758968588 1105 92-93 0.45590011122311935 1105 94-95 0.4467186283078668 1105 96-97 0.6705114052388588 1105 98-99 0.6517970626847678 1105 100-101 0.9755559346489058 1106 1 0.09267504320648356 1106 2 0.16155882775403185 1106 3 0.07523466937635703 1106 4 0.023560888355767418 1106 5 -0.038904777181329564 1106 6 0.016654202542596863 1106 7 0.15376484032343996 1106 8 0.06967595023817807 1106 9 0.1114158290041587 1106 10-11 0.16027202609831903 1106 12-13 0.22331581279658508 1106 14-15 0.23275214723539506 1106 16-17 0.2037804605620579 1106 18-19 0.12053520954600572 1106 20-21 0.021417419026903417 1106 22-23 -0.03402656617235067 1106 24-25 -0.0018589354318265805 1106 26-27 0.09769154387599599 1106 28-29 0.1177530843819028 1106 30-31 0.2120132658506435 1106 32-33 0.16198696920376676 1106 34-35 0.1599126490684526 1106 36-37 0.12982220121782717 1106 38-39 0.20605686775325438 1106 40-41 0.2628869451937348 1106 42-43 0.33004522612565523 1106 44-45 0.4339849475327284 1106 46-47 0.1885925971094906 1106 48-49 0.25294691154464743 1106 50-51 0.30229055647324543 1106 52-53 0.16417848017677983 1106 54-55 0.21491950501042822 1106 56-57 0.34820460714897195 1106 58-59 0.23228626187285073 1106 60-61 0.17956927150552104 1106 62-63 0.0870337594907511 1106 64-65 0.2836476753968995 1106 66-67 0.20928413227128928 1106 68-69 0.1885177692842035 1106 70-71 0.27344118485585867 1106 72-73 0.2563648243906762 1106 74-75 0.3037237442254046 1106 76-77 3.473456425489019E-4 1106 78-79 0.12132161958429677 1106 80-81 0.24251165696469812 1106 82-83 0.6310606699946106 1106 84-85 0.44053842669202226 1106 86-87 0.38353078665363327 1106 88-89 0.43522620649262933 1106 90-91 0.7628798834777797 1106 92-93 0.5597057229928026 1106 94-95 0.8741630766939466 1106 96-97 0.530958694626996 1106 98-99 1.002306757318987 1106 100-101 1.3018927744789224 1107 1 0.006554195775184013 1107 2 0.10120507853912741 1107 3 0.05694209351748469 1107 4 0.03259790438186627 1107 5 -0.015049464527265854 1107 6 -0.005900923039050099 1107 7 0.006966345007008101 1107 8 0.029993635672866503 1107 9 0.016512708203997306 1107 10-11 0.0493736057459202 1107 12-13 0.059461985916236415 1107 14-15 0.17200250686016716 1107 16-17 0.03556291627587882 1107 18-19 -0.06522864698227693 1107 20-21 -0.009478299918740163 1107 22-23 -0.07956701636178565 1107 24-25 -0.05560274752196648 1107 26-27 -0.05615613730397939 1107 28-29 -0.010307913382682443 1107 30-31 -0.09269731218286381 1107 32-33 -0.05881980592437941 1107 34-35 -0.08402951381286528 1107 36-37 -0.04209343384913211 1107 38-39 0.045684073620712695 1107 40-41 -0.004603515463657004 1107 42-43 -0.13408765607274375 1107 44-45 0.01762982060038354 1107 46-47 0.10024254882183925 1107 48-49 0.09770584475912614 1107 50-51 0.31335200672345564 1107 52-53 0.24112589054072942 1107 54-55 0.20404485703750197 1107 56-57 0.1357362642594211 1107 58-59 0.022878612839612344 1107 60-61 0.14208266035276296 1107 62-63 0.016776112821240474 1107 64-65 -0.020950118904480064 1107 66-67 0.2341320768384243 1107 68-69 0.2555326472819246 1107 70-71 0.2431900215625724 1107 72-73 0.3188379622772324 1107 74-75 0.26018611700602534 1107 76-77 0.2780450717334091 1107 78-79 0.2781076208944775 1107 80-81 0.5308515749770031 1107 82-83 0.550534571028571 1107 84-85 0.4936387636925126 1107 86-87 0.4884749013013341 1107 88-89 0.4120095063960534 1107 90-91 0.306896064904258 1107 92-93 0.3925075545465866 1107 94-95 0.6314454614958294 1107 96-97 0.6294523902189724 1107 98-99 0.891196816817466 1107 100-101 1.0142987148723819 1108 1 -0.019784082990280893 1108 2 0.07970519762177375 1108 3 0.07974151631690773 1108 4 -0.07658518868472441 1108 5 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-1.3439334647823387 2316 50-51 -1.4098478623474264 2316 52-53 -1.4403586145760912 2316 54-55 -1.0345459220870055 2316 56-57 -1.084006113451828 2316 58-59 -1.0360674077817222 2316 60-61 -0.9946964834093315 2316 62-63 -0.8813922273634027 2316 64-65 -1.278126671040262 2316 66-67 -1.8577048779043857 2316 68-69 -1.636941624184196 2316 70-71 -1.2884480364505748 2316 72-73 -0.8754712422321198 2316 74-75 -0.7241112461200991 2316 76-77 -1.0704838048755256 2316 78-79 -1.2102914818559967 2316 80-81 -1.5655655882896795 2316 82-83 -1.268378524771105 2316 84-85 -1.1333004794216741 2316 86-87 -1.3155053438056719 2316 88-89 -1.0335311056975591 2316 90-91 -1.5183208827144696 2316 92-93 -1.3192356318444887 2316 94-95 -1.383498556680209 2316 96-97 -1.5489556657724748 2316 98-99 -1.4143245409944747 2316 100-101 -1.645825475244397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 27.0 5 61.0 6 163.0 7 344.0 8 767.0 9 1594.0 10 2658.0 11 4329.0 12 6287.0 13 8623.0 14 10901.0 15 14498.0 16 18612.0 17 24048.0 18 20770.0 19 13361.0 20 11835.0 21 12316.0 22 13105.0 23 14392.0 24 16970.0 25 20236.0 26 24362.0 27 30169.0 28 36743.0 29 44702.0 30 54954.0 31 67366.0 32 82702.0 33 104681.0 34 135334.0 35 180156.0 36 246856.0 37 306794.0 38 178407.0 39 10101.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.963647207715695 19.244606723593673 14.911190957624775 20.880555111065853 2 15.655778327404041 22.292321545323617 38.54950242794687 23.50239769932548 3 21.338241136550433 25.59210361762412 30.056126019170915 23.013529226654537 4 12.738879436955605 17.26626823132516 37.881661795285666 32.113190536433564 5 12.825612020306837 38.974676947273885 33.42100854804261 14.778702484376671 6 29.809333248023822 37.193685545273176 17.206714633876793 15.790266572826205 7 27.516440461275195 34.14549735521749 20.522744431531716 17.81531775197559 8 26.50093006536632 32.68007729023507 19.337739946976388 21.481252697422224 9 26.22979235266369 17.650925161687354 21.182366582849248 34.93691590279971 10-11 25.28076587952951 26.614389265867317 26.464990641137348 21.639854213465828 12-13 27.83752389893318 24.32747078760763 26.930513215593827 20.90449209786537 14-15 23.305037177240305 25.689726075603904 27.790736023852997 23.2145007233028 16-17 21.01507231944569 28.950172698813038 28.059078233900152 21.97567674784112 18-19 20.757325646188942 27.973271817565266 30.599586792722803 20.66981574352299 20-21 22.546407295341993 25.497576246165444 30.449985284143988 21.506031174348575 22-23 21.56967330997555 25.90887556032651 30.753313244514814 21.768137885183126 24-25 22.40173520600482 25.94581436518141 30.82104990084163 20.83140052797214 26-27 21.886314596722286 25.30525871263029 32.0736361116629 20.73479057898452 28-29 20.676288261603766 27.433295775336084 31.11108073379446 20.779335229265687 30-31 21.35318390053117 25.813628569367825 30.95002245214668 21.88316507795432 32-33 21.335516869620953 26.178645724727268 31.13385967376665 21.35197773188513 34-35 22.54588236662847 25.723647088251205 30.80281780264911 20.92765274247122 36-37 22.235508262294548 25.36084639217598 31.470453411083334 20.933191934446143 38-39 21.541912849845758 26.31577722722458 31.376975694543415 20.765334228386244 40-41 21.659653215906385 26.595970850336187 30.604820333219617 21.13955560053781 42-43 21.21616284527872 26.89470325908097 29.630816640269142 22.258317255371164 44-45 21.963169808072234 25.97439121180051 29.657294072380147 22.40514490774711 46-47 22.847100175746924 26.96675582461603 29.15895368488341 21.02719031475364 48-49 22.678390958703716 26.863800351612426 28.89346783211597 21.56434085756788 50-51 21.44567226651249 27.435386170657054 29.645553142308056 21.4733884205224 52-53 22.40892163035844 28.0333354176452 28.0096905960211 21.548052355975262 54-55 21.638274747764996 27.017960010598074 28.57739486924148 22.766370372395453 56-57 22.699400598528978 27.010001657733323 28.128302742821575 22.162295000916117 58-59 23.7885813599624 26.644928176898414 27.818248232923693 21.748242230215492 60-61 22.368206750932135 27.150136403031695 29.416200841103556 21.06545600493261 62-63 22.093889695168237 27.132993435690416 29.140241224562207 21.632875644579137 64-65 21.659163635496366 28.357618567573528 28.35386656723381 21.629351229696297 66-67 22.26669722363923 27.29574282817578 27.710094212807984 22.727465735377006 68-69 21.894560503959724 27.37532609928542 28.26129823142943 22.46881516532543 70-71 22.87324554808873 27.30862329055361 27.816524049816387 22.001607111541276 72-73 22.554407651607224 26.619273094986944 28.979499720949114 21.846819532456717 74-75 21.674209197622066 28.774736205311957 27.740992589317916 21.81006200774806 76-77 22.198318797873295 27.31832759273063 27.727503676884385 22.755849932511683 78-79 22.36354990555049 27.444453654301704 28.02063201918365 22.171364420964156 80-81 22.882963689418258 27.453873401139923 27.734243780881823 21.928919128559997 82-83 22.440445531149926 27.231473017514645 28.421849837842462 21.906231613492974 84-85 21.934789791222165 27.896580166454548 28.05907344801029 22.109556594312995 86-87 22.06849295945053 28.043013429627077 27.402506072648418 22.48598753827397 88-89 21.888181956997535 27.922284351436446 26.933794818472006 23.25573887309401 90-91 22.31719809916389 27.092760871955342 27.311633924421464 23.27840710445931 92-93 22.81380918935504 27.77888777888778 27.503122786965584 21.9041802447916 94-95 22.267045989941845 27.418156796001863 27.670454082766305 22.644343131289983 96-97 21.22182698496208 27.580958928770333 28.47011189082494 22.727102195442644 98-99 21.8145907962865 28.061091684933544 26.898448772137286 23.22586874664267 100-101 21.3536016528916 27.586089558385474 27.254592189598583 23.805716599124345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 798.0 1 618.0 2 776.5 3 1037.5 4 774.5 5 655.0 6 993.5 7 1889.5 8 2849.0 9 3367.0 10 3151.5 11 2613.0 12 2533.0 13 2710.0 14 2946.5 15 3146.5 16 3343.0 17 3546.5 18 3753.0 19 4038.5 20 4474.5 21 4956.5 22 5596.5 23 6497.0 24 7550.0 25 8978.5 26 10852.5 27 13094.0 28 15264.5 29 17799.0 30 21092.5 31 24413.5 32 28496.0 33 31753.5 34 34270.0 35 36629.0 36 39615.5 37 43238.5 38 44714.0 39 45390.5 40 46327.5 41 48252.5 42 49923.0 43 51633.0 44 54173.5 45 56472.5 46 58659.0 47 72668.0 48 104581.0 49 101336.0 50 69612.0 51 56063.5 52 50514.0 53 48733.5 54 47422.5 55 44602.5 56 41001.5 57 37029.0 58 34165.0 59 30861.0 60 26159.5 61 21938.0 62 18441.5 63 14335.0 64 10542.5 65 8232.0 66 6252.0 67 4809.5 68 3552.0 69 2475.0 70 1879.0 71 1436.5 72 1165.0 73 1077.0 74 899.5 75 705.0 76 514.0 77 315.5 78 205.0 79 149.0 80 101.5 81 68.0 82 50.5 83 39.0 84 22.5 85 10.0 86 6.5 87 3.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0383310241654132 2 0.00500222773630582 3 0.0038970843992149995 4 5.234889491482835E-4 5 5.816543879425372E-4 6 2.3266175517701486E-4 7 0.0 8 0.0 9 0.001221474214679328 10-11 4.653235103540297E-4 12-13 8.724815819138058E-4 14-15 0.002152121235387388 16-17 0.0032572645724782082 18-19 0.0 20-21 0.0 22-23 0.002064873077196007 24-25 8.433988625166789E-4 26-27 0.004507821506554663 28-29 0.006689025461339177 30-31 0.0012796396534735818 32-33 2.6174447457414176E-4 34-35 0.00953913196225761 36-37 0.012796396534735818 38-39 0.009015643013109326 40-41 0.0021812039547845145 42-43 4.0715807155977603E-4 44-45 0.0011342260564879475 46-47 2.9082719397126858E-5 48-49 6.689025461339178E-4 50-51 0.0011633087758850743 52-53 0.0024138657099615295 54-55 0.003111850975492574 56-57 0.0011051433370908206 58-59 5.816543879425372E-4 60-61 0.003722588082832238 62-63 0.005176724052688581 64-65 0.008782981257932311 66-67 0.0023847829905644024 68-69 0.0028501065009184324 70-71 2.9082719397126858E-5 72-73 7.852334237224253E-4 74-75 0.00546755124665985 76-77 0.0027628583427270516 78-79 0.0035771744858466036 80-81 0.004624152384143171 82-83 0.004216994312583395 84-85 0.00500222773630582 86-87 0.002908271939712686 88-89 0.00852123678335817 90-91 0.011691253197644998 92-93 9.597297401051864E-4 94-95 0.001861294041416119 96-97 7.852334237224253E-4 98-99 0.0027628583427270516 100-101 0.0037807535216264915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1719234.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.00195731747407 #Duplication Level Percentage of deduplicated Percentage of total 1 78.37813665345527 43.10950926836497 2 12.92383968964009 14.216729579749233 3 3.8697774715204982 6.385360059500796 4 1.6277827511520244 3.581249496039366 5 0.8529591642887526 2.3457211773879165 6 0.519050654947093 1.7129281181404186 7 0.3398280206858875 1.3083844402332816 8 0.2358176911338863 1.0376347665961039 9 0.16741292661169552 0.8287234779500906 >10 0.9667394951940025 9.86344276495169 >50 0.07389803901085422 2.805219636265587 >100 0.03789647047690508 3.8153472229704937 >500 0.003588816061922339 1.3267058465805028 >1k 0.0023221750988909255 2.9902551782765348 >5k 7.388738951016581E-4 2.8094093568124063 >10k+ 2.1110682717190228E-4 1.8633796101806326 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21167 1.2311878429579683 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10929 0.6356900805823988 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9951 0.5788042814416188 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9578 0.557108572771362 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6274 0.36492996299514785 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6170 0.3588807573605455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5896 0.34294342713091996 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5507 0.32031707143995525 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5015 0.2916996755531824 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4785 0.278321624630504 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4122 0.23975793870991383 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3919 0.22795035463468033 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3911 0.2274850311243263 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3354 0.19508688171592697 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3115 0.18118534184410034 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2701 0.1571048501832793 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2332 0.13564180326819966 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2180 0.1268006565714731 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2150 0.1250556934076455 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1990 0.11574922320056491 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1828 0.10632642211589581 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1780 0.10353448105377162 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1747 0.10161502157356125 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1730 0.10062620911405894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 5.816543879425372E-5 9 0.0 0.0 0.0 0.0 1.1633087758850744E-4 10-11 0.0 0.0 0.0 0.0 1.1633087758850744E-4 12-13 0.0 0.0 0.0 0.0 1.1633087758850744E-4 14-15 0.0 0.0 0.0 0.0 1.1633087758850744E-4 16-17 0.0 0.0 0.0 0.0 1.1633087758850744E-4 18-19 0.0 0.0 0.0 0.0 1.1633087758850744E-4 20-21 0.0 0.0 0.0 0.0 1.1633087758850744E-4 22-23 0.0 0.0 0.0 5.816543879425372E-5 1.4541359698563432E-4 24-25 0.0 0.0 0.0 1.4541359698563432E-4 1.7449631638276117E-4 26-27 5.816543879425372E-5 0.0 0.0 5.525716685454104E-4 1.7449631638276117E-4 28-29 5.816543879425372E-5 0.0 0.0 8.724815819138058E-4 1.7449631638276117E-4 30-31 5.816543879425372E-5 0.0 0.0 0.0019485421996074995 1.7449631638276117E-4 32-33 5.816543879425372E-5 0.0 0.0 0.003170016414286828 1.7449631638276117E-4 34-35 5.816543879425372E-5 0.0 0.0 0.004362407909569029 1.7449631638276117E-4 36-37 5.816543879425372E-5 0.0 0.0 0.005642047563042611 1.7449631638276117E-4 38-39 5.816543879425372E-5 0.0 0.0 0.008753898538535185 1.7449631638276117E-4 40-41 5.816543879425372E-5 0.0 0.0 0.013319885483884103 1.7449631638276117E-4 42-43 5.816543879425372E-5 0.0 0.0 0.018031286026218653 1.7449631638276117E-4 44-45 5.816543879425372E-5 0.0 0.0 0.02009615910341466 1.7449631638276117E-4 46-47 5.816543879425372E-5 0.0 0.0 0.02454581517117507 1.7449631638276117E-4 48-49 5.816543879425372E-5 0.0 0.0 0.030653186244571713 1.7449631638276117E-4 50-51 5.816543879425372E-5 0.0 0.0 0.03838918960420745 1.7449631638276117E-4 52-53 5.816543879425372E-5 0.0 0.0 0.05188357140447432 1.7449631638276117E-4 54-55 5.816543879425372E-5 0.0 0.0 0.06212068863226297 1.7449631638276117E-4 56-57 5.816543879425372E-5 0.0 0.0 0.08870229416123693 1.7449631638276117E-4 58-59 5.816543879425372E-5 0.0 0.0 0.12921452228143465 2.326617551770149E-4 60-61 5.816543879425372E-5 0.0 0.0 0.16396837196100122 2.908271939712686E-4 62-63 5.816543879425372E-5 0.0 0.0 0.18528600527909522 3.4899263276552233E-4 64-65 5.816543879425372E-5 0.0 0.0 0.22635080506783833 3.4899263276552233E-4 66-67 5.816543879425372E-5 0.0 0.0 0.2982432874175359 3.4899263276552233E-4 68-69 5.816543879425372E-5 0.0 0.0 0.40052721153723114 3.7807535216264915E-4 70-71 5.816543879425372E-5 0.0 0.0 0.49027648359676457 5.816543879425372E-4 72-73 5.816543879425372E-5 0.0 0.0 0.5883724961232735 5.816543879425372E-4 74-75 5.816543879425372E-5 0.0 0.0 0.7045579601147953 5.816543879425372E-4 76-77 5.816543879425372E-5 0.0 0.0 0.7891595908410374 5.816543879425372E-4 78-79 5.816543879425372E-5 0.0 0.0 0.9003137443768562 5.816543879425372E-4 80-81 5.816543879425372E-5 0.0 0.0 1.0467743192607872 5.816543879425372E-4 82-83 5.816543879425372E-5 0.0 0.0 1.180409414890585 5.816543879425372E-4 84-85 5.816543879425372E-5 0.0 0.0 1.3496417590624663 5.816543879425372E-4 86-87 5.816543879425372E-5 0.0 0.0 1.5906211719870593 5.816543879425372E-4 88-89 5.816543879425372E-5 0.0 0.0 1.8870613308019735 5.816543879425372E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 160 1.906301E-9 29.6874 2 GTACACG 270 0.0 26.39955 1 GTACATG 10165 0.0 24.121891 1 GTACCTG 1520 0.0 23.44697 1 TACATGG 10275 0.0 23.391647 2 ATCACGC 185 2.5432564E-7 23.108028 3 TACCTGG 1460 0.0 22.773897 2 GAGTACT 10110 0.0 22.174698 12-13 ACATGGG 10215 0.0 21.76204 3 AGTACTT 10355 0.0 21.672977 12-13 GTACTTT 11050 0.0 20.22387 14-15 GCGTATC 120 0.004753323 19.799662 1 CATGGGG 3710 0.0 19.459719 4 TGGACCG 200 1.2860217E-5 18.99883 5 CATGGGA 6795 0.0 18.80311 4 CCGTCTA 155 9.6292625E-4 18.386501 9 TACTTTT 12195 0.0 18.188713 14-15 ACTTTTT 12735 0.0 17.77126 16-17 AGAGTAC 18625 0.0 17.53299 10-11 ATGGGAT 2635 0.0 17.124184 5 >>END_MODULE