##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936384_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1751812 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.288813525652294 31.0 31.0 34.0 28.0 34.0 2 31.314073656305585 31.0 31.0 34.0 28.0 34.0 3 31.25892789865579 31.0 31.0 34.0 27.0 34.0 4 34.925755160941925 37.0 35.0 37.0 32.0 37.0 5 34.98083926814064 37.0 35.0 37.0 32.0 37.0 6 35.05566065308378 37.0 35.0 37.0 32.0 37.0 7 34.93852593771478 37.0 35.0 37.0 32.0 37.0 8 34.92377549645738 37.0 35.0 37.0 32.0 37.0 9 36.38231157224634 39.0 37.0 39.0 32.0 39.0 10-11 36.24465096711291 39.0 35.0 39.0 32.0 39.0 12-13 36.14408737923932 39.0 35.0 39.0 31.5 39.0 14-15 37.05411939180688 39.0 36.0 41.0 31.0 41.0 16-17 36.99489043344833 39.0 36.0 41.0 31.0 41.0 18-19 36.933218861384674 39.0 36.0 41.0 31.0 41.0 20-21 36.9101807157389 39.0 36.0 41.0 31.0 41.0 22-23 36.903868679972504 39.0 36.0 41.0 30.5 41.0 24-25 36.98688158318359 39.0 36.0 41.0 31.0 41.0 26-27 36.89438535641953 39.0 36.0 41.0 30.0 41.0 28-29 36.788696503962754 39.0 36.0 41.0 30.0 41.0 30-31 36.58240924254429 39.0 36.0 41.0 30.0 41.0 32-33 36.35761286028409 39.0 35.0 40.5 30.0 41.0 34-35 36.107540649339086 38.5 35.0 40.0 30.0 41.0 36-37 35.866433156069256 38.0 35.0 40.0 28.5 41.0 38-39 35.647857475573865 38.0 35.0 40.0 27.5 41.0 40-41 35.431536317824055 38.0 35.0 40.0 27.0 41.0 42-43 35.115658244149486 38.0 34.0 40.0 26.0 41.0 44-45 34.88583135633276 38.0 34.0 40.0 25.0 41.0 46-47 34.63461347450526 38.0 34.0 40.0 24.0 41.0 48-49 34.31664071258788 38.0 33.0 40.0 23.0 41.0 50-51 33.30320605179095 37.0 32.0 39.5 21.0 40.5 52-53 33.411877244818506 37.5 33.0 39.5 21.0 40.5 54-55 33.854425303628474 38.0 33.0 40.0 21.5 41.0 56-57 33.66262532737531 38.0 33.0 40.0 19.5 41.0 58-59 33.42576343808582 37.5 33.0 40.0 18.5 41.0 60-61 33.086726201213374 37.0 32.0 40.0 17.5 41.0 62-63 32.78157045390715 36.5 32.0 40.0 14.5 41.0 64-65 32.431153285854876 36.0 31.5 39.0 13.0 41.0 66-67 32.01851226044804 36.0 31.0 39.0 9.0 41.0 68-69 31.570906295881066 35.0 31.0 39.0 7.5 40.0 70-71 31.128238646612765 35.0 30.0 38.0 7.0 40.0 72-73 30.709561299956846 35.0 30.0 37.0 2.0 39.5 74-75 30.312579489123262 35.0 30.0 37.0 2.0 39.0 76-77 29.855032389320314 34.0 29.0 36.0 2.0 39.0 78-79 29.371262155984773 34.0 29.0 36.0 2.0 38.5 80-81 28.871322664760832 34.0 28.5 35.0 2.0 37.0 82-83 28.444496327231462 34.0 27.5 35.0 2.0 37.0 84-85 28.06260175178615 34.0 27.0 35.0 2.0 36.0 86-87 27.760475153726542 34.0 26.0 35.0 2.0 36.0 88-89 27.37350155153635 33.5 26.0 35.0 2.0 36.0 90-91 26.961197891097903 33.0 25.0 35.0 2.0 35.0 92-93 26.51813921813528 33.0 25.0 35.0 2.0 35.0 94-95 26.014040319395004 33.0 23.5 35.0 2.0 35.0 96-97 25.407046817809217 32.0 19.5 35.0 2.0 35.0 98-99 24.680992309677066 32.0 10.0 35.0 2.0 35.0 100-101 22.127078704792524 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5391973051608367 1101 2 0.44962705876477926 1101 3 0.6280696790954607 1101 4 0.5404059888856665 1101 5 0.44991234038451466 1101 6 0.5485173541176849 1101 7 0.6390566039282604 1101 8 0.5677440565956147 1101 9 0.877071647732329 1101 10-11 0.8128150940962513 1101 12-13 0.7985471712978196 1101 14-15 1.0178619462500862 1101 16-17 1.0594640735059286 1101 18-19 1.1346900472774877 1101 20-21 1.1851394031512896 1101 22-23 0.9439895302135994 1101 24-25 0.9647465536738551 1101 26-27 0.8847852204007367 1101 28-29 0.8356726167313013 1101 30-31 0.6995760326942886 1101 32-33 0.8249684686416359 1101 34-35 0.8679411319560089 1101 36-37 0.7873425866109542 1101 38-39 0.8276883493019085 1101 40-41 0.7387681195946101 1101 42-43 0.7895513791536501 1101 44-45 0.8943090517973289 1101 46-47 1.1372678869091288 1101 48-49 1.1590259535736678 1101 50-51 0.9775447244473909 1101 52-53 0.933704496857132 1101 54-55 1.0120789830270667 1101 56-57 1.1131834173617534 1101 58-59 1.2610414328461488 1101 60-61 1.0990110048394897 1101 62-63 1.1241935913391288 1101 64-65 0.9223854217963208 1101 66-67 0.8534867379454134 1101 68-69 0.8770424772395131 1101 70-71 0.9679938154344256 1101 72-73 1.0993394140326807 1101 74-75 0.6961531243980197 1101 76-77 0.9808641505819402 1101 78-79 0.8936878415045086 1101 80-81 0.9322017387972963 1101 82-83 1.0416856197768425 1101 84-85 1.001050060342088 1101 86-87 1.1727315040246928 1101 88-89 1.138109324738128 1101 90-91 1.101434897366822 1101 92-93 1.1759350817082925 1101 94-95 1.336192096900902 1101 96-97 1.1702142824571418 1101 98-99 1.3225339493971298 1101 100-101 1.8114479198388018 1102 1 0.31521675743295674 1102 2 0.5077290863736899 1102 3 0.4366740651989396 1102 4 0.3970720270734063 1102 5 0.39296025935490064 1102 6 0.3725721303027498 1102 7 0.48728828504144417 1102 8 0.333105695260997 1102 9 0.5408405169628523 1102 10-11 0.5898838513895868 1102 12-13 0.5716284845809767 1102 14-15 0.7677481406063436 1102 16-17 0.579774134434409 1102 18-19 0.7417535559224717 1102 20-21 0.7334448031441596 1102 22-23 0.4774216366541495 1102 24-25 0.7005671348300169 1102 26-27 0.6486839469665497 1102 28-29 0.49365905517518627 1102 30-31 0.39694814147890156 1102 32-33 0.5311698570704877 1102 34-35 0.28329521188834406 1102 36-37 0.5172996292269261 1102 38-39 0.5889814362035537 1102 40-41 0.6662157477660884 1102 42-43 0.4581977544439013 1102 44-45 0.5696780283788456 1102 46-47 0.6071926223110111 1102 48-49 0.740323100109471 1102 50-51 0.5267355153626454 1102 52-53 0.5559963047192298 1102 54-55 0.7998318820984451 1102 56-57 0.5725620215610121 1102 58-59 0.5521244209407214 1102 60-61 0.6564565267572675 1102 62-63 0.6367423621532424 1102 64-65 0.7899249947363955 1102 66-67 0.7809611189361298 1102 68-69 0.8292666132083752 1102 70-71 0.5688464235370532 1102 72-73 0.5426370259826108 1102 74-75 0.39628671863503584 1102 76-77 0.4937093256472913 1102 78-79 0.5627401802104899 1102 80-81 0.6906653965786305 1102 82-83 0.502531382554924 1102 84-85 0.6827148670508443 1102 86-87 0.5361231160391675 1102 88-89 0.6404121904321833 1102 90-91 0.8706187919997816 1102 92-93 0.8290728901513127 1102 94-95 0.9891192799734156 1102 96-97 0.9513456464432615 1102 98-99 1.0089667030011178 1102 100-101 1.4871719020851195 1103 1 0.4636368453072599 1103 2 0.5248510718214945 1103 3 0.5445154562539436 1103 4 0.5304823491420265 1103 5 0.49169113263856445 1103 6 0.5623791862595908 1103 7 0.5051583425261867 1103 8 0.3678251848157359 1103 9 0.7527600189992683 1103 10-11 0.6467018417902892 1103 12-13 0.5844141927323605 1103 14-15 0.7979460607506823 1103 16-17 0.9653808307975211 1103 18-19 0.9357403159586966 1103 20-21 0.9929800982542929 1103 22-23 0.711341332543121 1103 24-25 0.6213397062951955 1103 26-27 0.747127355137458 1103 28-29 0.7680497520198912 1103 30-31 0.8251765804218323 1103 32-33 0.8454219686688091 1103 34-35 0.7427832036614817 1103 36-37 0.7370629799931834 1103 38-39 0.6558815543652514 1103 40-41 0.8962760657339857 1103 42-43 0.7861659007688928 1103 44-45 0.8490970174791954 1103 46-47 1.1668931339215831 1103 48-49 1.0470550028551386 1103 50-51 0.9701015744313466 1103 52-53 0.9993455190502942 1103 54-55 0.932664512509163 1103 56-57 1.0913517741062861 1103 58-59 0.9635727397668674 1103 60-61 0.8359858074676367 1103 62-63 0.8756500333848294 1103 64-65 1.0518381592854595 1103 66-67 0.9727065623655697 1103 68-69 1.1262987085087275 1103 70-71 1.1687151450407924 1103 72-73 1.0386848639329216 1103 74-75 1.159746975685735 1103 76-77 0.9406102943301633 1103 78-79 0.9137892069574463 1103 80-81 0.9537844786564094 1103 82-83 0.9628954387566999 1103 84-85 1.030224703963409 1103 86-87 1.0122082830326526 1103 88-89 0.9500469109158516 1103 90-91 1.1676206496153547 1103 92-93 1.2905277121507623 1103 94-95 1.426656645605565 1103 96-97 1.3771906930824755 1103 98-99 1.4554860417264237 1103 100-101 1.7739237368498415 1104 1 0.14605412115914618 1104 2 0.18171642450791126 1104 3 0.3203559693508602 1104 4 0.4189092293101737 1104 5 0.3534076671759152 1104 6 0.34108853516494975 1104 7 0.29060073423386257 1104 8 0.3001388608095539 1104 9 0.4762258608395271 1104 10-11 0.36791841560079064 1104 12-13 0.4116799148572241 1104 14-15 0.5232776480088503 1104 16-17 0.3568370249856301 1104 18-19 0.42171668706556886 1104 20-21 0.373928422915931 1104 22-23 0.41000117019604687 1104 24-25 0.38956204107035575 1104 26-27 0.08811200069052205 1104 28-29 -0.021622086965614074 1104 30-31 -0.12318302077964915 1104 32-33 -0.024605305855921245 1104 34-35 0.05171540737266156 1104 36-37 8.765652524758138E-4 1104 38-39 -0.08197044815906906 1104 40-41 -0.017504475910449457 1104 42-43 0.11721105405604959 1104 44-45 0.11851366225221227 1104 46-47 0.3584792003500823 1104 48-49 0.3522865925509464 1104 50-51 0.2690254901538722 1104 52-53 0.11506886273909345 1104 54-55 0.24542842129795162 1104 56-57 0.41500910640317556 1104 58-59 0.35987632391567814 1104 60-61 0.3149607241137744 1104 62-63 0.2008258822942608 1104 64-65 0.1824632253049785 1104 66-67 0.27568739436045675 1104 68-69 0.10878031106987862 1104 70-71 0.12634144884162168 1104 72-73 0.3615039176396273 1104 74-75 0.3716489065704103 1104 76-77 0.20546055529346674 1104 78-79 0.3123412465881188 1104 80-81 0.43041492374306856 1104 82-83 0.3714569851978773 1104 84-85 0.45894786618318406 1104 86-87 0.5111045721263636 1104 88-89 0.5530871943325621 1104 90-91 0.6901159029813364 1104 92-93 0.6992214330513882 1104 94-95 0.8115890280772824 1104 96-97 0.6773362745823839 1104 98-99 0.3127124365326175 1104 100-101 1.1038450009577048 1105 1 0.37915618480972313 1105 2 0.32566899243793657 1105 3 0.519160233636164 1105 4 0.5260841664386291 1105 5 0.4770829206227347 1105 6 0.4024908973573247 1105 7 0.4846016648895599 1105 8 0.48147189438113003 1105 9 0.4632113206514106 1105 10-11 0.5382229177784197 1105 12-13 0.5598690575560212 1105 14-15 0.8753013013030042 1105 16-17 0.7222877394468767 1105 18-19 0.725345982852005 1105 20-21 0.6638232274520988 1105 22-23 0.6442976624606302 1105 24-25 0.5104050687338244 1105 26-27 0.5268040111682382 1105 28-29 0.6715459591279824 1105 30-31 0.47115069771268736 1105 32-33 0.44165476535492587 1105 34-35 0.5221208177238523 1105 36-37 0.5708629191212253 1105 38-39 0.2704197161696271 1105 40-41 0.40955276987153866 1105 42-43 0.30549524911729975 1105 44-45 0.40697719152865375 1105 46-47 0.7709774357641521 1105 48-49 0.6810459448564146 1105 50-51 0.7362379149302285 1105 52-53 0.8240287765750196 1105 54-55 0.6830731826997933 1105 56-57 0.7033285931831585 1105 58-59 0.9646485485487801 1105 60-61 0.6524988260989915 1105 62-63 0.7705769580381343 1105 64-65 0.9287254401609601 1105 66-67 0.8895005730724925 1105 68-69 0.8647558526016894 1105 70-71 0.9071877181148196 1105 72-73 0.9397893152335186 1105 74-75 0.810589266179953 1105 76-77 0.9529302697703876 1105 78-79 1.1025372071241328 1105 80-81 1.129524265330307 1105 82-83 0.9277004823719253 1105 84-85 1.1082820290897573 1105 86-87 1.2578015286731095 1105 88-89 1.2004141734137228 1105 90-91 1.0733217504269987 1105 92-93 1.3898552723198314 1105 94-95 1.1601535700953427 1105 96-97 1.1985353564748564 1105 98-99 1.607466780028222 1105 100-101 2.063622636987674 1106 1 0.11139449662214318 1106 2 -0.03102958395530564 1106 3 0.12705953508854506 1106 4 0.26825813130778897 1106 5 0.26223020339785563 1106 6 0.075478539702587 1106 7 0.21784237917967175 1106 8 0.42490063491970176 1106 9 0.38303864642040253 1106 10-11 0.5211132279220578 1106 12-13 0.47818635724507885 1106 14-15 0.376953133166559 1106 16-17 0.17415443789995777 1106 18-19 0.32540589065769154 1106 20-21 0.13793468063255432 1106 22-23 -0.15029794443730538 1106 24-25 -0.17142642489209692 1106 26-27 -0.23617147492760182 1106 28-29 -0.1386202824638616 1106 30-31 -0.22650472861921145 1106 32-33 -0.0552694363709989 1106 34-35 0.018097592402682494 1106 36-37 -0.25760063576480263 1106 38-39 -0.026737129247678126 1106 40-41 -0.14154946182267025 1106 42-43 -0.06655739987638754 1106 44-45 -0.19396694726838604 1106 46-47 0.297892790042269 1106 48-49 0.17729477084838408 1106 50-51 -0.11759217707911773 1106 52-53 -0.10475928748830654 1106 54-55 0.023755669274486024 1106 56-57 -0.008711385772045332 1106 58-59 0.08938625922798593 1106 60-61 0.07211858108325941 1106 62-63 -0.1402521228343261 1106 64-65 -0.5505960910948815 1106 66-67 -0.40165239015459875 1106 68-69 -0.14988842039704764 1106 70-71 -0.4343111792938288 1106 72-73 -0.25962352661603916 1106 74-75 -0.21733439915017883 1106 76-77 -0.5000806369440092 1106 78-79 -0.22133487172810362 1106 80-81 -0.038226581907874646 1106 82-83 0.04337983483328145 1106 84-85 0.2225908492039821 1106 86-87 0.3447487687009776 1106 88-89 0.1962807403368032 1106 90-91 0.616321014681052 1106 92-93 0.7010860848669722 1106 94-95 0.687480726313936 1106 96-97 0.8597991029014551 1106 98-99 0.7150791302843977 1106 100-101 1.100168196719764 1107 1 0.13025712795397482 1107 2 0.06287542238176513 1107 3 0.14776037726939606 1107 4 0.2813253349461533 1107 5 0.19304744514048622 1107 6 0.29381492308156254 1107 7 0.3293819856163793 1107 8 0.21078027046907977 1107 9 0.3769378731852413 1107 10-11 0.5170284001686554 1107 12-13 0.25949859633319505 1107 14-15 0.06195519437683572 1107 16-17 0.1396744306166724 1107 18-19 0.46228278372332454 1107 20-21 0.2427647407503386 1107 22-23 0.3717174946925539 1107 24-25 0.2944625909541543 1107 26-27 0.19417815850430742 1107 28-29 0.3141040953626373 1107 30-31 0.19319872817874995 1107 32-33 0.1464410868490731 1107 34-35 0.2095647189118921 1107 36-37 0.2986636277591401 1107 38-39 0.38195061632021066 1107 40-41 0.3187352869602904 1107 42-43 0.2688833462101172 1107 44-45 0.15930122181427464 1107 46-47 0.27616031979172107 1107 48-49 0.23525671978151053 1107 50-51 0.15723799044663878 1107 52-53 0.056484767991491935 1107 54-55 0.13746785782969795 1107 56-57 0.4485436907893927 1107 58-59 0.49202552901739693 1107 60-61 0.3840073145999696 1107 62-63 0.5071084283629688 1107 64-65 0.48231345808824955 1107 66-67 0.5062319693000141 1107 68-69 0.3937014458135266 1107 70-71 0.47466733501853753 1107 72-73 0.45125260538734224 1107 74-75 0.3941759258254507 1107 76-77 0.4782224397319901 1107 78-79 0.5529603017664684 1107 80-81 0.3196203637672177 1107 82-83 0.2657983607661727 1107 84-85 0.33228943172553116 1107 86-87 0.5681319205830171 1107 88-89 0.5811570319177584 1107 90-91 0.3439268345568429 1107 92-93 0.33177960284655583 1107 94-95 0.397679804446593 1107 96-97 0.4557623785788678 1107 98-99 0.9279462737742961 1107 100-101 1.5466021750806362 1108 1 -0.025321668613702997 1108 2 0.015489188548926336 1108 3 0.10919257108672298 1108 4 0.11873979707213778 1108 5 0.13983833113166355 1108 6 0.05451576675149283 1108 7 0.500989663203498 1108 8 0.3201641116785794 1108 9 -0.3191224043929779 1108 10-11 0.12510480199374285 1108 12-13 0.10228189120230269 1108 14-15 0.4029619028349387 1108 16-17 0.4677487343929485 1108 18-19 0.5077685879859715 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90-91 -5.933747512032884 2316 92-93 -5.314916242744314 2316 94-95 -4.600281321371469 2316 96-97 -5.8287936876288455 2316 98-99 -7.183902156945901 2316 100-101 -7.830503824473748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5079.0 3 1964.0 4 1934.0 5 2066.0 6 2307.0 7 2701.0 8 3425.0 9 4333.0 10 5364.0 11 6995.0 12 8972.0 13 10943.0 14 12313.0 15 14994.0 16 18481.0 17 22870.0 18 18125.0 19 13492.0 20 13326.0 21 14041.0 22 15512.0 23 17254.0 24 19642.0 25 22527.0 26 26260.0 27 30326.0 28 35953.0 29 42791.0 30 51319.0 31 62737.0 32 77678.0 33 98473.0 34 127874.0 35 170385.0 36 227598.0 37 286233.0 38 221525.0 39 33991.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.416140691219994 18.2496199780984 14.113107999339638 21.221131331341965 2 15.295948857241884 21.126421988279784 39.526006761706654 24.051622392771677 3 21.04506541293072 25.582550462015398 29.640806935511954 23.731577189541923 4 12.350367864104388 16.438381192398122 38.61231359858222 32.59893734491527 5 12.925363112414967 38.55860444242445 33.56296099203717 14.953071453123412 6 29.898155190567877 36.45040347354981 17.809537187402217 15.841904148480094 7 27.697396420971447 33.854867964409664 20.76830574558334 17.67942986903555 8 26.208034487367083 32.32328251381288 19.873870724917786 21.59481227390225 9 26.550021278358056 17.65886650615073 21.428648952790606 34.36246326270061 10-11 25.156842407079726 26.288438098654215 26.709245248748857 21.845474245517195 12-13 27.677714590494723 24.19573267410695 26.925349064768252 21.201203670630075 14-15 23.50222203947932 25.277312354448135 27.696257311143775 23.52420829492877 16-17 21.24889519052698 28.76428399065035 27.80373157877452 22.183089240048147 18-19 20.95755990817559 27.97072218774888 30.084659731957014 20.987058172118513 20-21 22.77606525684864 25.67415881092494 29.697244443124927 21.852531489101494 22-23 21.729519263980908 26.05331742393318 30.04321799969742 22.173945312388494 24-25 22.537095444403082 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28.60914487955246 22.026715378467863 50-51 21.37997862201932 27.428149006523284 29.459085713144034 21.732786658313366 52-53 22.250624567768853 27.988656313764356 27.846032521193067 21.914686597273736 54-55 21.462303867255088 26.953906557271306 28.541720539487418 23.042069035986188 56-57 22.605326365093344 26.93004025956789 27.908743076595933 22.555890298742835 58-59 23.545501667486803 26.651873739109387 27.741420235217824 22.061204358185986 60-61 22.301842038787427 27.051096703111643 29.335784247264485 21.311277010836445 62-63 21.878751586262723 27.263339306377187 28.961147052762392 21.896762054597694 64-65 21.452913682124255 28.391503844037345 28.247766302622896 21.9078161712155 66-67 21.987607670811236 27.369414311316387 27.56581123689534 23.07716678097704 68-69 21.59704091631352 27.414309677960745 28.209967298574547 22.778682107151184 70-71 22.6282749471396 27.295117434569562 27.63751124567869 22.43909637261215 72-73 22.358050254119686 26.674102459207106 28.902584932328484 22.06526235434473 74-75 21.45217300701237 28.707385897327935 27.78457335584676 22.055867739812935 76-77 21.948674466274973 27.308135916453974 27.731433106653647 23.011756510617403 78-79 22.140789503733984 27.44536049512412 28.068576289766366 22.345273711375523 80-81 22.636342798552313 27.51270464229328 27.58346756594668 22.26748499320773 82-83 22.226687666942777 27.254537389758543 28.448390527497786 22.070384415800902 84-85 21.906488352431495 27.883605948146382 27.953878973979574 22.256026725442545 86-87 21.928201152768352 28.125344292072707 27.589261912006226 22.357192643152718 88-89 21.648868670827365 27.92508115988855 26.971084987975154 23.454965181308935 90-91 22.070793277464436 27.141980453497133 27.371868791816045 23.415357477222386 92-93 22.63951346194797 27.723489002598136 27.641451644486516 21.995545890967385 94-95 22.057724108460285 27.36547155285439 27.851800977454488 22.725003361230836 96-97 21.044275342585408 27.449993063512533 28.734042510677337 22.771689083224718 98-99 21.64925408926263 28.030519305521935 27.193682140622215 23.12654446459322 100-101 21.391441721898357 27.207733092851754 27.720326545666524 23.68049863958337 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 413.0 1 342.0 2 565.0 3 806.5 4 632.5 5 572.0 6 936.0 7 1841.0 8 2822.0 9 3254.5 10 2994.0 11 2516.5 12 2409.0 13 2543.5 14 2710.5 15 2850.5 16 2985.5 17 3203.0 18 3413.0 19 3584.5 20 3967.0 21 4511.0 22 5171.0 23 6098.0 24 7183.5 25 8672.0 26 10543.5 27 12888.0 28 15135.0 29 17658.5 30 20897.5 31 24387.0 32 28662.0 33 32000.0 34 34750.0 35 37406.0 36 40591.0 37 44325.5 38 45765.5 39 46227.5 40 47584.0 41 49765.0 42 51304.0 43 53257.0 44 55866.5 45 58442.0 46 61183.5 47 73992.5 48 103481.5 49 101773.0 50 72455.0 51 58723.0 52 52845.5 53 50856.0 54 49230.5 55 45662.5 56 42069.0 57 38287.0 58 35125.0 59 31633.5 60 26919.5 61 22746.0 62 19429.5 63 15149.0 64 10981.5 65 8551.0 66 6503.5 67 4972.0 68 3652.5 69 2524.5 70 1860.5 71 1444.0 72 1214.0 73 1103.0 74 933.5 75 739.5 76 540.0 77 322.5 78 200.5 79 140.0 80 93.0 81 71.0 82 48.0 83 33.5 84 23.0 85 13.5 86 8.0 87 5.0 88 3.5 89 4.0 90 4.0 91 4.0 92 3.5 93 1.0 94 2.5 95 2.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07164010750011988 2 0.027343116727137386 3 0.01409968649603953 4 0.01997931284863901 5 0.02431767792434348 6 0.02968355051797796 7 0.07632097508180101 8 0.051946213406461425 9 0.06890008745230652 10-11 0.05765458850607257 12-13 0.038160487540900505 14-15 0.040986133215208026 16-17 0.05391560281582727 18-19 0.04917765148315002 20-21 0.019008889081705112 22-23 0.012958011476117299 24-25 0.021920160382506798 26-27 0.024603096679324036 28-29 0.005651291348615034 30-31 0.001141675019922229 32-33 0.00901923265738561 34-35 0.010817370813763121 36-37 0.004024404445225858 38-39 0.009989656424319504 40-41 0.0035391925617589103 42-43 0.0017981381563775109 44-45 1.7125125298833436E-4 46-47 2.283350039844458E-4 48-49 6.850050119533374E-4 50-51 5.422956344630588E-4 52-53 2.8541875498055726E-5 54-55 5.708375099611145E-5 56-57 0.0031110644292880745 58-59 0.004224197573712248 60-61 0.004823576959171417 62-63 0.003025438802793907 64-65 4.566700079688916E-4 66-67 1.4270937749027862E-4 68-69 0.0030539806782919625 70-71 0.001598345027891121 72-73 0.021320780997047626 74-75 0.015098652138471478 76-77 0.025231017940281264 78-79 0.018951805330709004 80-81 0.010760287062767009 82-83 0.00508045383865392 84-85 0.004167113822716136 86-87 0.001655428778887232 88-89 0.0013129262729105636 90-91 0.005251705091642254 92-93 0.009761321420335058 94-95 0.012644050845638688 96-97 0.01170216895420285 98-99 0.022605165394460135 100-101 0.018637844700230388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1751812.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.42064982127212 #Duplication Level Percentage of deduplicated Percentage of total 1 79.90380668749752 45.881285031894166 2 12.100104152198073 13.895916866485724 3 3.579941382986295 6.1668768159941 4 1.4833935088605796 3.4070967687772584 5 0.7735766438709379 2.2209636788814024 6 0.4715496486829859 1.6246012350221772 7 0.3028385946865749 1.2172432228504786 8 0.20754225579600372 0.9533768954543369 9 0.16485310990290422 0.8519375426114115 >10 0.9058532709115864 9.655633790765807 >50 0.0683323428582859 2.6833139776212453 >100 0.03250754114407101 3.489171743823869 >500 0.0027004076546682334 1.0013950419476056 >1k 0.0023003472613840506 3.11393255516907 >5k 5.000754916052284E-4 2.1057608535351755 >10k+ 2.0003019664209133E-4 1.7314939791661588 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18009 1.0280212716889712 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12141 0.6930538208437892 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9610 0.548574847072631 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7634 0.4357773551043148 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7335 0.4187093135564775 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7008 0.40004292698074906 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5080 0.2899854550602462 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4639 0.26481152087096105 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4375 0.2497414106079876 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4047 0.23101794028126307 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3896 0.2223982938808502 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3304 0.18860471329115225 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3270 0.18666386575728447 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3151 0.17987089938874717 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2781 0.15874991152018597 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2166 0.12364340465757741 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2156 0.12307256714761629 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1955 0.11159873319739791 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1934 0.11039997442647954 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1822 0.10400659431491507 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1812 0.10343575680495395 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1783 0.10178032802606672 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 2.854187549805573E-5 0.0 26-27 0.0 0.0 0.0 2.5687687948250154E-4 0.0 28-29 0.0 0.0 0.0 5.708375099611146E-4 0.0 30-31 0.0 0.0 0.0 0.0014270937749027864 0.0 32-33 0.0 0.0 0.0 0.0026258525458211267 0.0 34-35 0.0 0.0 0.0 0.0037960694412414115 0.0 36-37 0.0 0.0 0.0 0.005851084477101424 0.0 38-39 0.0 0.0 0.0 0.009704237669338946 0.0 40-41 0.0 0.0 0.0 0.014584898379506477 0.0 42-43 0.0 0.0 0.0 0.01912305658369734 0.0 44-45 0.0 0.0 0.0 0.021520574125534018 0.0 46-47 0.0 0.0 0.0 0.026486860462195713 0.0 48-49 0.0 0.0 0.0 0.03190981680682631 0.0 50-51 0.0 0.0 0.0 0.0388740344283519 0.0 52-53 0.0 0.0 0.0 0.05126120839450809 0.0 54-55 0.0 0.0 0.0 0.06182170232878871 0.0 56-57 0.0 0.0 0.0 0.08651042463460691 0.0 58-59 0.0 0.0 0.0 0.1279817697332819 0.0 60-61 0.0 0.0 0.0 0.16320244409788265 0.0 62-63 0.0 0.0 0.0 0.18871888079314447 0.0 64-65 0.0 0.0 0.0 0.22913417649839138 0.0 66-67 0.0 0.0 0.0 0.30071720024751514 0.0 68-69 0.0 0.0 0.0 0.3937637143711768 0.0 70-71 0.0 0.0 0.0 0.48204373528666317 0.0 72-73 0.0 0.0 0.0 0.5786294419720838 1.1416750199222292E-4 74-75 0.0 0.0 0.0 0.6943667471166998 1.1416750199222292E-4 76-77 0.0 0.0 0.0 0.7817905117672443 1.1416750199222292E-4 78-79 0.0 0.0 0.0 0.8964146837674363 1.1416750199222292E-4 80-81 0.0 0.0 0.0 1.0424063769399914 1.4270937749027865E-4 82-83 0.0 0.0 0.0 1.1758681867688998 1.7125125298833436E-4 84-85 0.0 0.0 0.0 1.353141775487324 1.7125125298833436E-4 86-87 0.0 0.0 0.0 1.596347096606257 1.7125125298833436E-4 88-89 0.0 0.0 0.0 1.8895006998467871 1.7125125298833436E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1590 0.0 30.18947 2 GTACCTG 1760 0.0 27.27422 1 GTACATG 11160 0.0 24.572834 1 TACATGG 11265 0.0 23.921206 2 GAGTACT 8655 0.0 22.652332 12-13 ACATGGG 11450 0.0 22.456797 3 AGTACTT 8940 0.0 21.61121 12-13 ATAACGC 160 4.9680224E-5 20.793812 3 GTACTTT 9440 0.0 20.714148 14-15 ACCGTCG 115 0.0037091875 20.666428 8 CATGGGG 3795 0.0 20.665838 4 GTATTAG 555 0.0 20.55237 1 TATAACG 165 6.298568E-5 20.162542 2 GTGTAGC 905 0.0 18.905909 1 ACTTTTT 11015 0.0 18.419394 16-17 TACTTTT 10540 0.0 18.304363 14-15 CATGGGA 7655 0.0 18.192957 4 AGAGTAC 17450 0.0 17.814327 10-11 CGAACGG 80 0.0011920964 17.811563 30-31 TAACGCA 245 3.7403079E-6 17.460526 4 >>END_MODULE