FastQCFastQC Report
Thu 26 May 2016
SRR936384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936384_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751812
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT215771.2316960952430969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110210.6291200197281444No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT101570.5797996588675041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97090.5542261384212461No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT63980.3652218388731211No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC62870.35888554251255267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61810.3528346649069649No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT55340.3159014780124808No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC51620.2946663226419273No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC49430.2821649811737789No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT42050.24003717293864865No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG39460.2252524814306558No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA38260.2184024313111224No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA35260.201277306012289No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC32140.18346717570150223No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.1574940689982715No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC24990.1426522937392825No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21300.12158838962171739No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21250.12130297086673685No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT19680.11234082196034734No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19010.10851621064360786No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.10075282050813672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15600.025.8842952
GTACCTG16700.025.0391641
GTACATG107600.024.8627831
TACATGG109600.023.7093032
GAGTACT108200.022.8680212-13
ACATGGG108900.022.5074673
TCGACCG652.946638E-421.91926418-19
GTACTTT115250.021.34552814-15
AGTACTT110300.021.119412-13
TAAGGTG7450.021.038415
CATGGGG35100.019.6207754
ACTTTTT130550.019.00760816-17
CGTCGAT1001.2877335E-518.99696744-45
TACTTTT123550.018.83524914-15
CATGGGA72800.018.7243064
GTAAGGT7500.018.3650444
CATATAG5200.018.2697033
TATACGT1300.007568523818.2676165
GTGTAGC8400.018.1019061
GTACACG2956.317168E-817.7183911
Produced by FastQC (version 0.11.5)