##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936384_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1751812 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.817438743426806 31.0 31.0 34.0 30.0 34.0 2 31.966627697492655 31.0 31.0 34.0 30.0 34.0 3 32.04359771482328 33.0 31.0 34.0 30.0 34.0 4 35.74900388854512 37.0 35.0 37.0 35.0 37.0 5 35.597985400259844 37.0 35.0 37.0 33.0 37.0 6 35.70134808986352 37.0 35.0 37.0 33.0 37.0 7 35.649815733651785 37.0 35.0 37.0 33.0 37.0 8 35.62714663445621 37.0 35.0 37.0 33.0 37.0 9 37.22263347893495 39.0 37.0 39.0 33.0 39.0 10-11 37.10651656684622 39.0 37.0 39.0 33.0 39.0 12-13 37.00900153669458 39.0 37.0 39.0 33.0 39.0 14-15 38.04152072254328 40.0 37.0 41.0 33.0 41.0 16-17 37.97038095412064 40.0 37.0 41.0 33.0 41.0 18-19 38.17970649818588 40.0 37.5 41.0 33.0 41.0 20-21 38.21481899884235 40.0 37.5 41.0 33.0 41.0 22-23 38.12393167760011 40.0 37.0 41.0 33.0 41.0 24-25 37.96838958746714 40.0 37.0 41.0 33.0 41.0 26-27 37.74958129068644 39.5 37.0 41.0 32.5 41.0 28-29 37.65888976671013 39.0 37.0 41.0 32.5 41.0 30-31 37.59278392886908 39.0 37.0 41.0 32.0 41.0 32-33 37.37968029674417 39.0 36.0 41.0 32.0 41.0 34-35 37.15091488127722 39.0 36.0 41.0 31.0 41.0 36-37 36.90818735115412 39.0 35.0 41.0 31.0 41.0 38-39 36.720218265430304 39.0 35.0 41.0 31.0 41.0 40-41 36.539193418015174 39.0 35.0 40.5 30.0 41.0 42-43 36.211955963311134 39.0 35.0 40.0 30.0 41.0 44-45 35.863412854804054 38.5 35.0 40.0 29.5 41.0 46-47 35.40287171226136 38.0 35.0 40.0 27.5 41.0 48-49 34.932147113959715 38.0 34.0 40.0 25.5 41.0 50-51 34.604194114436936 38.0 34.0 40.0 24.0 41.0 52-53 34.26126005530274 38.0 33.5 40.0 23.0 41.0 54-55 34.075135630992364 38.0 33.0 40.0 23.0 41.0 56-57 33.739055047002765 37.5 33.0 40.0 22.5 41.0 58-59 33.3641606519421 37.0 32.5 40.0 21.5 41.0 60-61 32.89690760195729 36.5 32.0 39.0 19.0 41.0 62-63 32.59578853210276 36.0 31.0 39.0 18.5 40.0 64-65 32.18606933849066 35.5 31.0 39.0 17.5 40.0 66-67 31.72252273645802 35.0 31.0 38.0 14.5 40.0 68-69 31.3439972439965 35.0 30.0 38.0 13.0 40.0 70-71 30.94143321315301 35.0 30.0 37.0 9.0 39.5 72-73 30.46011529776026 34.0 29.5 37.0 7.5 39.0 74-75 29.976047087244524 34.0 29.0 36.0 6.5 39.0 76-77 28.512873527524643 32.5 27.0 34.5 2.0 37.0 78-79 29.26927061808002 34.0 29.0 35.0 2.0 37.0 80-81 29.003137037535993 34.0 29.0 35.0 2.0 37.0 82-83 28.677533319785457 34.0 28.5 35.0 2.0 36.5 84-85 28.293534351859677 33.0 27.5 35.0 2.0 36.0 86-87 27.94266079921818 33.0 27.0 35.0 2.0 36.0 88-89 27.441978933812532 33.0 26.0 35.0 2.0 35.0 90-91 26.916517868355736 32.5 25.5 35.0 2.0 35.0 92-93 26.293039721157292 32.0 24.5 34.0 2.0 35.0 94-95 25.727634586359724 32.0 23.5 34.0 2.0 35.0 96-97 24.97005043920238 32.0 19.5 34.0 2.0 35.0 98-99 24.088226647608305 31.0 8.5 34.0 2.0 35.0 100-101 20.919439985569227 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29951634402754834 1101 2 0.32724296573093525 1101 3 0.2983043924193467 1101 4 0.2311726216723713 1101 5 0.2412797278337706 1101 6 0.29037215042770725 1101 7 0.24659529927144774 1101 8 0.2935537732169209 1101 9 0.292544470451503 1101 10-11 0.40883052400905484 1101 12-13 0.4097138290262379 1101 14-15 0.5451971103954705 1101 16-17 0.5433404195518179 1101 18-19 0.4895414290717781 1101 20-21 0.3881937720996689 1101 22-23 0.37247207403709837 1101 24-25 0.2898766123763252 1101 26-27 0.40074841041231224 1101 28-29 0.34949821454536334 1101 30-31 0.31371009745598855 1101 32-33 0.34660342256726295 1101 34-35 0.3966781368509871 1101 36-37 0.5286178893849467 1101 38-39 0.4335394179693637 1101 40-41 0.48662444518166126 1101 42-43 0.6450398036262897 1101 44-45 0.4706336569321721 1101 46-47 0.6136904214778056 1101 48-49 0.36200832990057563 1101 50-51 0.4001889804705385 1101 52-53 0.4756720213629251 1101 54-55 0.42475513178655433 1101 56-57 0.40643687210658896 1101 58-59 0.35528585021090464 1101 60-61 0.4430303733291652 1101 62-63 0.36611800004451567 1101 64-65 0.3142266731776715 1101 66-67 0.18286902885352063 1101 68-69 0.058387620829460474 1101 70-71 0.07439839696676032 1101 72-73 0.1443180010092604 1101 74-75 0.18502677715782667 1101 76-77 0.07498903274355939 1101 78-79 0.22867663057467524 1101 80-81 0.13435258834630304 1101 82-83 0.15672605203634404 1101 84-85 0.22334046476732894 1101 86-87 0.3284343198739208 1101 88-89 0.2732070261812929 1101 90-91 0.21630775588977613 1101 92-93 0.27164289243783557 1101 94-95 0.17533123916967952 1101 96-97 0.5071082656095669 1101 98-99 0.2767214371932276 1101 100-101 0.7416647487091552 1102 1 0.3217976206211297 1102 2 0.35029370436353346 1102 3 0.22551888888033034 1102 4 0.26932129260929827 1102 5 0.3136703088030899 1102 6 0.23475091599317466 1102 7 0.23501684896459807 1102 8 0.2552717628317325 1102 9 0.37514868329863305 1102 10-11 0.4090831895240754 1102 12-13 0.41010577906046564 1102 14-15 0.4602900318542851 1102 16-17 0.4710717125367978 1102 18-19 0.27962989172739583 1102 20-21 0.4026428423754993 1102 22-23 0.32125358928280434 1102 24-25 0.34580529190812825 1102 26-27 0.44190613654158284 1102 28-29 0.386775083293486 1102 30-31 0.37827441717850263 1102 32-33 0.4977487829489746 1102 34-35 0.4347253169339851 1102 36-37 0.39872106184465395 1102 38-39 0.42402263333550394 1102 40-41 0.5258767760741208 1102 42-43 0.5600932328281374 1102 44-45 0.556790049666958 1102 46-47 0.557396544731013 1102 48-49 0.6610611102403965 1102 50-51 0.6906379692139737 1102 52-53 0.725136115682318 1102 54-55 0.7238355142414648 1102 56-57 0.7290092023875516 1102 58-59 0.6353882922761898 1102 60-61 0.6650002131349808 1102 62-63 0.748494771463335 1102 64-65 0.6453055506803338 1102 66-67 0.4406706605207056 1102 68-69 0.5563565298070223 1102 70-71 0.5551707635975873 1102 72-73 0.6115835911085092 1102 74-75 0.5889480610213766 1102 76-77 0.5531413216808012 1102 78-79 0.6067784713384974 1102 80-81 0.6888438346539907 1102 82-83 0.6457055891021675 1102 84-85 0.568196619529413 1102 86-87 0.6017971747309581 1102 88-89 0.5411517981708407 1102 90-91 0.4564363902390376 1102 92-93 0.5249041884972776 1102 94-95 0.5186535656288136 1102 96-97 0.7490713789922587 1102 98-99 0.753830790828129 1102 100-101 1.149524217423643 1103 1 0.21592430243701344 1103 2 0.23690293875916169 1103 3 0.18837817539800028 1103 4 0.2359377840492698 1103 5 0.24847537514788343 1103 6 0.25651652616341636 1103 7 0.257763596066269 1103 8 0.13746795211024931 1103 9 0.31255142910132605 1103 10-11 0.219840570349632 1103 12-13 0.28335368472109934 1103 14-15 0.37399485516541375 1103 16-17 0.36317315983112763 1103 18-19 0.3324091491727046 1103 20-21 0.23837185638673475 1103 22-23 0.21072380571559535 1103 24-25 0.3269723229457995 1103 26-27 0.2683665407766256 1103 28-29 0.27585124332291144 1103 30-31 0.36009549347991765 1103 32-33 0.23801024641425528 1103 34-35 0.2998667616436208 1103 36-37 0.23335641055419387 1103 38-39 0.2628705471699746 1103 40-41 0.31289729821127565 1103 42-43 0.40787534697525984 1103 44-45 0.2819232818766295 1103 46-47 0.17407603825117945 1103 48-49 0.08143341207931343 1103 50-51 0.21478186303800584 1103 52-53 0.22592022441206439 1103 54-55 0.2738283483243862 1103 56-57 0.29078727766364665 1103 58-59 0.4511890151639619 1103 60-61 0.30465703336214034 1103 62-63 0.34309702716700485 1103 64-65 0.2810505257708371 1103 66-67 0.1488981274394341 1103 68-69 0.31015025467281276 1103 70-71 0.2692793911684497 1103 72-73 0.2717809120220238 1103 74-75 0.23462466580710384 1103 76-77 0.20986904085638258 1103 78-79 0.06195379606223739 1103 80-81 0.16437409180685947 1103 82-83 0.07868300141356954 1103 84-85 0.38999158372606146 1103 86-87 0.45645442712228856 1103 88-89 0.3615173068860571 1103 90-91 0.3629454730044124 1103 92-93 0.4487916557087175 1103 94-95 0.344538625423489 1103 96-97 0.3790700894033314 1103 98-99 0.35794728571989154 1103 100-101 0.7202549946094621 1104 1 0.15511836325351425 1104 2 0.22804210383910473 1104 3 0.19891638004631318 1104 4 0.21349439832465578 1104 5 0.25782671815414204 1104 6 0.20264189147141565 1104 7 0.20271212115639514 1104 8 0.19873623105252847 1104 9 0.20119282191677712 1104 10-11 0.21864842714618504 1104 12-13 0.21049075342744317 1104 14-15 0.16224446446529583 1104 16-17 0.3925440581570143 1104 18-19 0.1606083534174445 1104 20-21 0.1940090429125263 1104 22-23 0.12952020558667243 1104 24-25 0.1977996863090965 1104 26-27 0.18442411250429558 1104 28-29 0.1151222253412243 1104 30-31 0.05021166074561734 1104 32-33 0.08756605355871727 1104 34-35 0.04962367439492965 1104 36-37 0.06410511928015694 1104 38-39 -0.027868708915328 1104 40-41 -0.023978157154111557 1104 42-43 -0.03788951791156592 1104 44-45 0.04803759653310635 1104 46-47 0.06714243154060995 1104 48-49 0.26086349097771944 1104 50-51 0.2076439005289572 1104 52-53 0.28161279962014163 1104 54-55 0.08609050878092717 1104 56-57 0.03458469321960678 1104 58-59 0.07462899089586728 1104 60-61 0.12952221812199127 1104 62-63 0.013780840400158922 1104 64-65 -0.028071001602555157 1104 66-67 -0.015947258236430173 1104 68-69 -0.09767783185812462 1104 70-71 0.034013322356475584 1104 72-73 0.06943142975046257 1104 74-75 0.006334997895436345 1104 76-77 -0.1899196966879373 1104 78-79 -0.18743747013116518 1104 80-81 0.2196859883207587 1104 82-83 0.24885518031495124 1104 84-85 0.46990567032741737 1104 86-87 0.5052037804695786 1104 88-89 0.34849094512347634 1104 90-91 0.34447057125499825 1104 92-93 0.4905953495515014 1104 94-95 0.6161139391346921 1104 96-97 0.6636293769613282 1104 98-99 0.8809673096088027 1104 100-101 1.110518910169482 1105 1 -0.04161493069251776 1105 2 -0.001032521153707222 1105 3 0.049699856202181536 1105 4 0.17528052528072635 1105 5 0.2304994253688193 1105 6 0.16072840062373217 1105 7 -0.02018514595747689 1105 8 0.18098005238755377 1105 9 0.11567288340279447 1105 10-11 0.04500951109865525 1105 12-13 0.06197431271491638 1105 14-15 0.06036929780996303 1105 16-17 0.1439152067848184 1105 18-19 0.17408090168867574 1105 20-21 0.15086975303418626 1105 22-23 0.07892338340987948 1105 24-25 0.03307964113569284 1105 26-27 -0.013942273048584752 1105 28-29 0.002767509583343042 1105 30-31 0.29942504754808397 1105 32-33 0.3267900540188222 1105 34-35 0.29365283842560785 1105 36-37 0.22213202464082826 1105 38-39 0.16827665113862622 1105 40-41 0.23065107066307888 1105 42-43 0.18264215836664732 1105 44-45 0.29567775694049914 1105 46-47 0.24692733505044373 1105 48-49 0.3718917609515202 1105 50-51 0.4610256314439809 1105 52-53 0.4670634617973519 1105 54-55 0.33075100244672484 1105 56-57 0.30924759195067253 1105 58-59 0.2567627543524651 1105 60-61 0.3476705269951168 1105 62-63 0.3162217202357809 1105 64-65 0.3820117117293549 1105 66-67 0.14385390560602573 1105 68-69 0.35702903774028627 1105 70-71 0.45774373545257774 1105 72-73 0.3533271649423213 1105 74-75 0.34228967810017963 1105 76-77 0.21763403822158267 1105 78-79 0.2687432862095811 1105 80-81 0.3078235266841638 1105 82-83 0.5196318072631847 1105 84-85 0.6128698087127837 1105 86-87 0.8128814285248751 1105 88-89 0.5840874797381197 1105 90-91 0.7343379266765915 1105 92-93 0.5792005685431505 1105 94-95 0.8768198780774057 1105 96-97 0.7506276463600052 1105 98-99 0.5770538501315379 1105 100-101 0.9542267888496241 1106 1 0.0570310295895311 1106 2 0.14917165862314263 1106 3 0.06669599867383624 1106 4 0.06997688621549969 1106 5 0.1543533160879349 1106 6 0.005022567473652373 1106 7 -0.0216396828177281 1106 8 -0.16714338440920073 1106 9 -0.0753133038918179 1106 10-11 0.04219163493826272 1106 12-13 0.05454697790323593 1106 14-15 0.14485291924194854 1106 16-17 0.009332783714455672 1106 18-19 0.20322044885554646 1106 20-21 -0.01817635645220861 1106 22-23 -0.007793936008674507 1106 24-25 -0.016922568157305307 1106 26-27 -0.038418927500778466 1106 28-29 -0.3110230001159664 1106 30-31 -0.12907323947915472 1106 32-33 -0.05123205329733338 1106 34-35 -0.06821240086650704 1106 36-37 -0.3165239237007569 1106 38-39 -0.06904406216501258 1106 40-41 0.010957946599461366 1106 42-43 0.05961742045045071 1106 44-45 -0.06033645241004848 1106 46-47 0.042128185474112456 1106 48-49 -0.263322108887202 1106 50-51 -0.1517297474138033 1106 52-53 -0.32170706150709094 1106 54-55 -0.29490998719323613 1106 56-57 -0.48029875285111956 1106 58-59 -0.37482042501540747 1106 60-61 -0.46496138834888256 1106 62-63 -0.3393065987902375 1106 64-65 -0.41895642101730246 1106 66-67 -0.12017253603472611 1106 68-69 -0.3055954790410951 1106 70-71 -0.22088890741675016 1106 72-73 -0.12312922155858885 1106 74-75 -0.146636626050622 1106 76-77 -0.3009643554682633 1106 78-79 -0.3962821669010026 1106 80-81 -0.05265448211601509 1106 82-83 -0.14647131842400896 1106 84-85 0.13907639096732893 1106 86-87 0.05409126820213572 1106 88-89 -0.03482823008203795 1106 90-91 0.10023178607512762 1106 92-93 0.1316039710598922 1106 94-95 0.24521126615051259 1106 96-97 0.4091608607682993 1106 98-99 0.5632863581826122 1106 100-101 0.991705132640277 1107 1 0.03127792063999024 1107 2 0.05848505567157147 1107 3 0.011181417273419925 1107 4 0.13003098914916933 1107 5 0.06263176053397501 1107 6 -0.03043024922389037 1107 7 -0.01595191883704672 1107 8 -0.02552059304971266 1107 9 -0.03598536073416625 1107 10-11 -0.002571024115056275 1107 12-13 -0.07336288327450546 1107 14-15 0.11777114801275701 1107 16-17 0.06589408144835573 1107 18-19 -0.08941233553777295 1107 20-21 0.043262158617125124 1107 22-23 -0.049576662964298635 1107 24-25 -0.015337149660020089 1107 26-27 -0.0445620245945193 1107 28-29 -0.04814043590901207 1107 30-31 -0.06570035784581307 1107 32-33 -0.0058479623626723765 1107 34-35 0.06869134419400069 1107 36-37 0.019952286454710588 1107 38-39 -0.19608852190798842 1107 40-41 -0.21398044725230392 1107 42-43 -0.05991922152601603 1107 44-45 -0.07870398410307189 1107 46-47 -0.14854591211984314 1107 48-49 -0.15408512494418858 1107 50-51 -0.16517940017914867 1107 52-53 -0.10208638557332961 1107 54-55 -0.10651715836370101 1107 56-57 -0.16082548158255605 1107 58-59 -0.2322052606453937 1107 60-61 -0.07510469105218931 1107 62-63 -0.11836953577449094 1107 64-65 0.020535421602389192 1107 66-67 0.030857404195291593 1107 68-69 0.015340075148277066 1107 70-71 -0.05133850698957332 1107 72-73 0.010097718203947892 1107 74-75 0.07163454534080316 1107 76-77 -0.06651281129954612 1107 78-79 0.14747507674428206 1107 80-81 0.2638704401154932 1107 82-83 0.25022611636655867 1107 84-85 0.5301300479021513 1107 86-87 0.4236960630989266 1107 88-89 0.22234726228125723 1107 90-91 0.35321900657143246 1107 92-93 0.6565686542933058 1107 94-95 0.5109181487515428 1107 96-97 0.9444098580380889 1107 98-99 0.8525534368185497 1107 100-101 1.1676603731778883 1108 1 0.03309490508688029 1108 2 0.07398174223791543 1108 3 9.632307929052786E-4 1108 4 0.1285093739482761 1108 5 -0.030558428650692804 1108 6 0.05450072708935494 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-0.9087224087321673 2316 58-59 -1.6086485033579052 2316 60-61 -1.142802375746097 2316 62-63 -1.0208445832626225 2316 64-65 -0.7302740480788472 2316 66-67 -0.49237212870275116 2316 68-69 -1.2763563935473208 2316 70-71 -1.5271811145129028 2316 72-73 -0.84298627253634 2316 74-75 -0.9541523603966695 2316 76-77 -0.9270929406787438 2316 78-79 -1.1397242752268042 2316 80-81 -1.0124013543883486 2316 82-83 -1.3042906375977061 2316 84-85 -1.130686483632612 2316 86-87 -0.9818155814066145 2316 88-89 -1.154045187955333 2316 90-91 -1.2790149560347324 2316 92-93 -1.5710490464125044 2316 94-95 -1.4598765552359474 2316 96-97 -1.755837684474649 2316 98-99 -2.1806595554813875 2316 100-101 -3.1993626590385134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 19.0 5 68.0 6 149.0 7 365.0 8 810.0 9 1587.0 10 2558.0 11 4440.0 12 6324.0 13 8670.0 14 10934.0 15 14699.0 16 18793.0 17 24116.0 18 21315.0 19 13890.0 20 12177.0 21 12463.0 22 13212.0 23 14881.0 24 17341.0 25 20687.0 26 25074.0 27 30080.0 28 37562.0 29 45893.0 30 56003.0 31 69173.0 32 85050.0 33 106560.0 34 138323.0 35 183374.0 36 251005.0 37 311122.0 38 182496.0 39 10581.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.839430553129 19.330396344979654 14.938331237931457 20.891841863959886 2 15.703410721891032 22.320754695437415 38.43761739307168 23.538217189599873 3 21.22108407219841 25.61585410062266 30.055895135678863 23.10716669150007 4 12.75913168764685 17.296376551821773 37.859142419392036 32.08534934113935 5 12.802588021324802 38.93254966567966 33.47587544662149 14.788986866374046 6 29.85662845099817 37.2739768879309 17.12889282639918 15.740501834671758 7 27.552499925791125 34.08796149358493 20.532911065799297 17.82662751482465 8 26.622434370811483 32.648309293462994 19.30224247807413 21.427013857651392 9 26.218395581261 17.654805424326355 21.105917758298265 35.02088123611438 10-11 25.32985845513103 26.615470153189953 26.460744646114993 21.593926745564023 12-13 27.907240261343443 24.328852499536904 26.8555187266543 20.90838851246536 14-15 23.30018666407887 25.681666390761553 27.77027759859802 23.247869346561558 16-17 21.03288510207862 28.939129581008 28.007608124504262 22.020377192409114 18-19 20.78914274395232 27.901080439573988 30.601151852365614 20.70862496410808 20-21 22.530728183161205 25.4550431210655 30.452297392642592 21.561931303130702 22-23 21.625930680799105 25.866176050014584 30.708684565497723 21.79920870368859 24-25 22.3958107376819 25.892104860567226 30.80841437323183 20.90367002851904 26-27 21.891691940944675 25.323656324620487 31.974972899009636 20.809678835425206 28-29 20.70516079146845 27.45064739305948 31.046124275833996 20.798067539638073 30-31 21.38207599676901 25.803109399840736 30.92157427994874 21.893240323441518 32-33 21.385062599296567 26.129062275039576 31.128300156933538 21.357574968730322 34-35 22.58599917626431 25.697813446985645 30.769896028343812 20.94629134840623 36-37 22.188935843740243 25.329104161945338 31.49615738317309 20.98580261114133 38-39 21.512605809538407 26.355647918145507 31.297189191750274 20.834557080565812 40-41 21.62807132512734 26.5717895044494 30.566545782102956 21.2335933883203 42-43 21.269401072476814 26.79636441377271 29.642192803210754 22.292041710539724 44-45 22.008415397059807 25.984721904293266 29.63812993459829 22.368732764048637 46-47 22.885821774570807 26.936547609771495 29.12771051875218 21.049920096905513 48-49 22.683599653500742 26.87018978968865 28.88276822653174 21.56344233027887 50-51 21.44088520914345 27.44466872015947 29.598838231499723 21.515607839197358 52-53 22.444712865386578 27.984443509537854 27.92616089996113 21.64468272511444 54-55 21.609090091748616 26.99912518427501 28.565031060953107 22.826753663023265 56-57 22.794239803791605 26.927701248053932 28.16933250438026 22.108726443774202 58-59 23.82640520487486 26.617202465406937 27.797463223014272 21.75892910670393 60-61 22.386705646354958 27.13243797282403 29.425658595823435 21.055197784997578 62-63 22.066319103094163 27.210265685176367 29.097659198160336 21.625756013569138 64-65 21.66600106249267 28.381485679899676 28.30854992587957 21.64396333172808 66-67 22.275996021195553 27.24841696957242 27.75030575999018 22.725281249241842 68-69 21.95776815469293 27.379287432512506 28.177834760241716 22.48510965255285 70-71 22.81521156813961 27.32420126252168 27.830448652723195 22.030138516615516 72-73 22.589530894162984 26.619159652736613 28.963475807756712 21.82783364534369 74-75 21.695226411987015 28.802049097625837 27.679743440373034 21.822981050014114 76-77 22.208848569402882 27.313638766379 27.700643800098874 22.776868864119248 78-79 22.35952580217552 27.385974787546086 28.088046063365674 22.166453346912725 80-81 22.945867213603776 27.41025564171623 27.69000426759383 21.953872877086162 82-83 22.455485192560655 27.23730154742765 28.40680722011108 21.900406039900613 84-85 21.95841808877374 27.89028542063679 27.996802489490324 22.154494001099145 86-87 22.063310509914587 28.06050227886893 27.425467555625836 22.450719655590643 88-89 21.86420352339891 27.930502812437542 26.957970476543984 23.247323187619564 90-91 22.30648140560232 27.05918095619357 27.340950162999544 23.293387475204565 92-93 22.789275775997115 27.810506619632296 27.461660148337547 21.93855745603304 94-95 22.304042555074556 27.428746316015168 27.676871955350517 22.59033917355976 96-97 21.230847163656932 27.61140904967323 28.460203190894873 22.697540595774964 98-99 21.861287918376437 28.092708877676024 26.843740451693783 23.20226275225376 100-101 21.424924266308444 27.556983034739265 27.244681507271807 23.773411191680488 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 863.0 1 680.0 2 877.5 3 1171.0 4 873.5 5 678.5 6 964.5 7 1876.5 8 2943.0 9 3407.5 10 3108.0 11 2664.5 12 2564.0 13 2723.5 14 2972.0 15 3146.0 16 3366.5 17 3561.5 18 3752.0 19 4036.0 20 4443.0 21 5102.0 22 5731.0 23 6525.0 24 7641.5 25 8996.0 26 10940.5 27 13206.5 28 15510.0 29 18112.0 30 21292.0 31 24669.5 32 28659.0 33 32003.0 34 34717.0 35 37129.5 36 40125.0 37 44071.5 38 45960.0 39 46128.0 40 46769.5 41 49161.0 42 51120.5 43 53022.5 44 55521.5 45 57667.5 46 59804.5 47 73928.5 48 106228.5 49 103235.0 50 71098.5 51 57120.0 52 51932.0 53 50201.0 54 48569.0 55 45469.5 56 41966.0 57 38163.0 58 34992.0 59 31456.5 60 26530.0 61 22342.0 62 18941.5 63 14714.0 64 10749.5 65 8409.0 66 6399.0 67 4902.5 68 3651.0 69 2453.0 70 1794.0 71 1399.0 72 1141.0 73 1047.5 74 893.0 75 715.0 76 529.0 77 319.0 78 210.0 79 162.0 80 115.5 81 79.5 82 56.0 83 40.5 84 17.5 85 8.0 86 4.0 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0204359828566079 2 0.012444257717152298 3 0.007934641388459493 4 0.0 5 0.0027971037988094613 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.989656424319504E-4 14-15 1.141675019922229E-4 16-17 2.8541875498055726E-5 18-19 0.0014270937749027864 20-21 0.0 22-23 1.7125125298833436E-4 24-25 0.0 26-27 0.0017695962808794551 28-29 0.0031681481802841856 30-31 0.0015412612768950095 32-33 0.006564631364552817 34-35 0.002083556911358068 36-37 6.27921260957226E-4 38-39 0.0011702168954202848 40-41 1.7125125298833436E-4 42-43 0.004138571947218081 44-45 0.0013129262729105636 46-47 1.4270937749027862E-4 48-49 2.5687687948250154E-4 50-51 0.0 52-53 1.7125125298833436E-4 54-55 8.27714389443616E-4 56-57 0.01170216895420285 58-59 0.021520574125534018 60-61 0.017210750925327603 62-63 0.010845912689261177 64-65 0.015441154644448148 66-67 0.001683970654385288 68-69 3.425025059766687E-4 70-71 2.8541875498055726E-5 72-73 2.8541875498055726E-5 74-75 9.41881891435839E-4 76-77 0.0014841775258988977 78-79 0.007877557637463381 80-81 0.013671558363568693 82-83 0.01484177525898898 84-85 0.0256591460727521 86-87 0.01001819829981756 88-89 0.037789443159425784 90-91 0.041870931355647754 92-93 0.026743737341678217 94-95 0.027485826104627666 96-97 0.045581375170395 98-99 0.0483499370937064 100-101 0.05365872593634476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1751812.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.63403388700273 #Duplication Level Percentage of deduplicated Percentage of total 1 78.22118778933816 42.73539024364305 2 12.979872032733239 14.182855369706134 3 3.8915121816001186 6.378270252036744 4 1.665420467213095 3.639545529673126 5 0.8624454842126326 2.3559437905082725 6 0.505101314982491 1.6557433415473832 7 0.3376237750008557 1.2912024135118174 8 0.24067856658076406 1.0519392769958966 9 0.1835216102712475 0.902387328710098 >10 0.9891087036119935 9.977983841951737 >50 0.07845193941777666 2.926496000914899 >100 0.03827855018369766 3.8960045137055586 >500 0.003346503312715564 1.2149251214656795 >1k 0.0025098774845366727 3.104732463339213 >5k 6.274693711341682E-4 2.2437605551673583 >10k+ 3.137346855670841E-4 2.4428199571230778 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21577 1.2316960952430969 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11021 0.6291200197281444 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10157 0.5797996588675041 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9709 0.5542261384212461 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6398 0.3652218388731211 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6287 0.35888554251255267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6181 0.3528346649069649 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5534 0.3159014780124808 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5162 0.2946663226419273 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4943 0.2821649811737789 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4205 0.24003717293864865 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3946 0.2252524814306558 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3826 0.2184024313111224 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3526 0.201277306012289 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3214 0.18346717570150223 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2759 0.1574940689982715 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2499 0.1426522937392825 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2130 0.12158838962171739 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2125 0.12130297086673685 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1968 0.11234082196034734 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1901 0.10851621064360786 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.10075282050813672 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 2.854187549805573E-5 16-17 0.0 0.0 0.0 0.0 5.708375099611146E-5 18-19 0.0 0.0 0.0 0.0 1.1416750199222292E-4 20-21 0.0 0.0 0.0 0.0 1.1416750199222292E-4 22-23 0.0 0.0 0.0 0.0 1.1416750199222292E-4 24-25 0.0 0.0 0.0 0.0 1.1416750199222292E-4 26-27 0.0 0.0 0.0 1.9979312848639007E-4 1.1416750199222292E-4 28-29 0.0 0.0 0.0 4.5667000796889166E-4 1.1416750199222292E-4 30-31 0.0 0.0 0.0 0.0013129262729105636 1.1416750199222292E-4 32-33 0.0 0.0 0.0 0.0026258525458211267 1.1416750199222292E-4 34-35 0.0 0.0 0.0 0.0037389856902453004 1.1416750199222292E-4 36-37 0.0 0.0 0.0 0.005736916975109202 1.1416750199222292E-4 38-39 0.0 0.0 0.0 0.00967569579384089 1.1416750199222292E-4 40-41 0.0 0.0 0.0 0.014584898379506477 1.1416750199222292E-4 42-43 0.0 0.0 0.0 0.01883763782871678 1.1416750199222292E-4 44-45 0.0 2.854187549805573E-5 0.0 0.021063904117565126 1.1416750199222292E-4 46-47 0.0 5.708375099611146E-5 0.0 0.02614435795621905 1.1416750199222292E-4 48-49 0.0 5.708375099611146E-5 0.0 0.03133897929686519 1.1416750199222292E-4 50-51 0.0 5.708375099611146E-5 0.0 0.038274655042892736 1.1416750199222292E-4 52-53 0.0 5.708375099611146E-5 0.0 0.050547661507056696 1.7125125298833436E-4 54-55 0.0 5.708375099611146E-5 0.0 0.06110815544133731 2.2833500398444583E-4 56-57 0.0 5.708375099611146E-5 0.0 0.08579687774715553 2.2833500398444583E-4 58-59 0.0 5.708375099611146E-5 0.0 0.12684009471335966 2.2833500398444583E-4 60-61 0.0 5.708375099611146E-5 0.0 0.1620607690779604 2.2833500398444583E-4 62-63 0.0 5.708375099611146E-5 0.0 0.18737741264473584 2.2833500398444583E-4 64-65 0.0 5.708375099611146E-5 0.0 0.22747874771950416 2.2833500398444583E-4 66-67 0.0 5.708375099611146E-5 0.0 0.2989761458421337 2.2833500398444583E-4 68-69 0.0 5.708375099611146E-5 0.0 0.39239370434727017 2.2833500398444583E-4 70-71 0.0 5.708375099611146E-5 0.0 0.4795035083673362 2.5687687948250154E-4 72-73 0.0 5.708375099611146E-5 0.0 0.5752615006633132 3.425025059766687E-4 74-75 0.0 5.708375099611146E-5 0.0 0.6899712982899991 3.425025059766687E-4 76-77 0.0 5.708375099611146E-5 0.0 0.7774236048160419 3.425025059766687E-4 78-79 0.0 5.708375099611146E-5 0.0 0.891676732434759 3.425025059766687E-4 80-81 0.0 5.708375099611146E-5 0.0 1.0369548787198626 3.710443814747245E-4 82-83 0.0 5.708375099611146E-5 0.0 1.1688468853963783 4.5667000796889166E-4 84-85 0.0 5.708375099611146E-5 0.0 1.3440940009544402 4.5667000796889166E-4 86-87 0.0 5.708375099611146E-5 0.0 1.5851872232865172 4.5667000796889166E-4 88-89 0.0 5.708375099611146E-5 0.0 1.8754010133507477 4.5667000796889166E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1560 0.0 25.884295 2 GTACCTG 1670 0.0 25.039164 1 GTACATG 10760 0.0 24.862783 1 TACATGG 10960 0.0 23.709303 2 GAGTACT 10820 0.0 22.86802 12-13 ACATGGG 10890 0.0 22.507467 3 TCGACCG 65 2.946638E-4 21.919264 18-19 GTACTTT 11525 0.0 21.345528 14-15 AGTACTT 11030 0.0 21.1194 12-13 TAAGGTG 745 0.0 21.03841 5 CATGGGG 3510 0.0 19.620775 4 ACTTTTT 13055 0.0 19.007608 16-17 CGTCGAT 100 1.2877335E-5 18.996967 44-45 TACTTTT 12355 0.0 18.835249 14-15 CATGGGA 7280 0.0 18.724306 4 GTAAGGT 750 0.0 18.365044 4 CATATAG 520 0.0 18.269703 3 TATACGT 130 0.0075685238 18.267616 5 GTGTAGC 840 0.0 18.101906 1 GTACACG 295 6.317168E-8 17.718391 1 >>END_MODULE