##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936383_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 939064 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.846676051898488 33.0 31.0 34.0 30.0 34.0 2 31.885368835350945 33.0 31.0 34.0 30.0 34.0 3 31.894119037680074 33.0 31.0 34.0 30.0 34.0 4 35.51972176550267 37.0 35.0 37.0 33.0 37.0 5 35.55775431706465 37.0 35.0 37.0 33.0 37.0 6 35.60518665394478 37.0 35.0 37.0 33.0 37.0 7 35.56473467197124 37.0 35.0 37.0 33.0 37.0 8 35.54509916257039 37.0 35.0 37.0 33.0 37.0 9 37.077190692008216 39.0 37.0 39.0 33.0 39.0 10-11 36.97043598732355 39.0 37.0 39.0 33.0 39.0 12-13 36.899782655921214 39.0 37.0 39.0 33.0 39.0 14-15 37.93992901442287 40.0 38.0 41.0 33.0 41.0 16-17 37.92158681410426 40.0 37.5 41.0 33.0 41.0 18-19 37.99967201383505 40.0 38.0 41.0 33.0 41.0 20-21 37.892072851264665 40.0 38.0 41.0 33.0 41.0 22-23 37.99131262618948 40.0 38.0 41.0 33.0 41.0 24-25 37.98007430803438 40.0 38.0 41.0 33.0 41.0 26-27 37.85096862407674 40.0 37.5 41.0 33.0 41.0 28-29 37.73038259373163 40.0 37.0 41.0 33.0 41.0 30-31 37.505243519078576 39.5 37.0 41.0 32.5 41.0 32-33 37.19625446188971 39.0 36.0 41.0 32.0 41.0 34-35 36.91093897753508 39.0 35.0 41.0 31.0 41.0 36-37 36.643302799383214 39.0 35.0 41.0 30.5 41.0 38-39 36.40574497584829 39.0 35.0 40.0 30.0 41.0 40-41 36.22649201758347 39.0 35.0 40.0 30.0 41.0 42-43 35.89501674007309 38.0 35.0 40.0 29.5 41.0 44-45 35.49102989785574 38.0 35.0 40.0 28.0 41.0 46-47 35.04076931923703 38.0 35.0 40.0 25.5 41.0 48-49 34.67499233279095 38.0 34.0 40.0 24.0 41.0 50-51 33.59897727950384 37.5 33.0 39.5 22.5 40.5 52-53 33.71221450295187 37.5 33.5 39.5 22.0 40.5 54-55 34.14145521498002 38.0 34.0 40.0 22.0 41.0 56-57 33.941931007897225 38.0 34.0 40.0 21.0 41.0 58-59 33.68792169649779 38.0 34.0 40.0 20.0 41.0 60-61 33.27711955734647 37.0 33.0 40.0 18.0 41.0 62-63 32.991022976069786 37.0 33.0 40.0 16.5 41.0 64-65 32.63578254517264 36.0 32.5 39.5 12.5 41.0 66-67 32.05937188519633 35.5 32.0 39.0 8.0 40.5 68-69 31.656255058228194 35.0 31.0 38.5 7.0 40.0 70-71 31.149230510380548 35.0 31.0 37.5 2.0 40.0 72-73 30.745319275363553 35.0 30.5 37.0 2.0 39.0 74-75 30.361780986173468 35.0 30.0 36.5 2.0 39.0 76-77 29.922767777276096 34.5 30.0 36.0 2.0 39.0 78-79 29.41368958878202 34.0 29.0 35.5 2.0 37.5 80-81 28.888305269928352 34.0 29.0 35.0 2.0 37.0 82-83 28.44114032696387 34.0 28.0 35.0 2.0 36.5 84-85 28.085562325890464 34.0 27.0 35.0 2.0 36.0 86-87 27.861403482616733 34.0 27.0 35.0 2.0 36.0 88-89 27.40096787865364 34.0 26.0 35.0 2.0 35.5 90-91 26.910595550463015 33.0 25.0 35.0 2.0 35.0 92-93 26.432047230007754 33.0 24.5 35.0 2.0 35.0 94-95 25.905505375565458 33.0 23.5 35.0 2.0 35.0 96-97 25.298795396266918 32.5 19.5 35.0 2.0 35.0 98-99 24.636855421994667 32.0 8.5 35.0 2.0 35.0 100-101 21.9385734092671 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2578220138427376 1101 2 0.3539292885015719 1101 3 0.32515516607911366 1101 4 0.4175697775789047 1101 5 0.20460027956229965 1101 6 0.4724753932491552 1101 7 0.46336153768783106 1101 8 0.4278503908716189 1101 9 0.3447417117539828 1101 10-11 0.48558191676435314 1101 12-13 0.44705037788875046 1101 14-15 0.639376696002941 1101 16-17 0.7536278698418357 1101 18-19 0.4585867040086953 1101 20-21 0.46003841960989433 1101 22-23 0.5171888486692708 1101 24-25 0.43178546768266557 1101 26-27 0.4046591440901963 1101 28-29 0.47854832181290163 1101 30-31 0.38644317275008433 1101 32-33 0.13395155724751362 1101 34-35 0.13394573306884894 1101 36-37 0.10175506220650021 1101 38-39 0.23652964113074404 1101 40-41 0.13973370902816384 1101 42-43 0.20319902803876744 1101 44-45 0.18146454912455567 1101 46-47 0.4018301286395527 1101 48-49 0.4754588841920011 1101 50-51 0.4195683622367241 1101 52-53 0.613221397931774 1101 54-55 0.5779885180703772 1101 56-57 0.464681761998186 1101 58-59 0.5652580146673003 1101 60-61 0.6035007919179591 1101 62-63 0.4724301382465441 1101 64-65 0.3788556195773509 1101 66-67 0.5543086206462498 1101 68-69 0.4879469476778411 1101 70-71 0.7064986349290123 1101 72-73 0.6382464840145872 1101 74-75 0.7001788564208447 1101 76-77 0.7305955329415816 1101 78-79 0.6727269790759287 1101 80-81 0.8287531095302825 1101 82-83 0.4664864223196723 1101 84-85 0.5645664735527802 1101 86-87 0.5162235785081677 1101 88-89 0.7226299593564356 1101 90-91 0.7605748644483512 1101 92-93 0.5873207934578488 1101 94-95 0.6816540854770352 1101 96-97 0.5939922501060693 1101 98-99 0.6182172151326952 1101 100-101 0.8678522794237935 1102 1 0.20854771926114424 1102 2 0.26449739590170296 1102 3 0.20832335605092567 1102 4 0.20471635654875087 1102 5 0.2062463744912577 1102 6 0.3669260068421636 1102 7 0.2586776413010554 1102 8 0.3499424974460368 1102 9 0.3490082118536435 1102 10-11 0.3330903518886359 1102 12-13 0.429695643541784 1102 14-15 0.32193551942766874 1102 16-17 0.30684011287306134 1102 18-19 0.2970153684829455 1102 20-21 0.2530261886796765 1102 22-23 0.10488500090012565 1102 24-25 0.18580371887141922 1102 26-27 -0.1607724620001747 1102 28-29 -0.1818258133199251 1102 30-31 0.18036943837464037 1102 32-33 0.188460211257933 1102 34-35 0.22395064466019932 1102 36-37 -0.16145529448892404 1102 38-39 -0.013351573100067071 1102 40-41 -0.03309802803798334 1102 42-43 -0.15905530341535012 1102 44-45 -0.0076797662423189195 1102 46-47 0.1926713009438643 1102 48-49 0.29910695087575334 1102 50-51 0.19135554084781603 1102 52-53 0.21711223247520905 1102 54-55 0.18983777670481317 1102 56-57 0.14286197291188785 1102 58-59 0.04991596503887763 1102 60-61 0.008357172359858112 1102 62-63 0.11215312271453115 1102 64-65 0.11149821588725217 1102 66-67 0.36441066073622963 1102 68-69 0.30863360060628864 1102 70-71 0.1869870713345776 1102 72-73 -0.005102803092842834 1102 74-75 -0.005903330874506452 1102 76-77 0.11560223299283479 1102 78-79 0.23507282451028289 1102 80-81 0.2424810518582312 1102 82-83 0.32772996250930575 1102 84-85 0.09653608397104918 1102 86-87 0.003691902303049943 1102 88-89 -0.13310872796738593 1102 90-91 0.12157193528915755 1102 92-93 -0.17116193411255054 1102 94-95 -0.2383376929461285 1102 96-97 -0.26167480984381797 1102 98-99 -0.16909931201630357 1102 100-101 -0.10760466763067811 1103 1 0.22347059999954766 1103 2 0.14124320595756856 1103 3 0.166029209827947 1103 4 0.2874222103257722 1103 5 0.3565833917368266 1103 6 0.3046233592832621 1103 7 0.4639583371961251 1103 8 0.38455887466245287 1103 9 0.10739569060029908 1103 10-11 0.4115986710530777 1103 12-13 0.034888289886204404 1103 14-15 0.3626375627844851 1103 16-17 0.4264148183625025 1103 18-19 0.4453295232072634 1103 20-21 0.467610913600744 1103 22-23 0.254218177363569 1103 24-25 0.43917389132708706 1103 26-27 0.2181119653741277 1103 28-29 0.24712730788483128 1103 30-31 0.17472591760709122 1103 32-33 0.29761318976170514 1103 34-35 0.3304450580680225 1103 36-37 0.4678693806385965 1103 38-39 0.5601280540556388 1103 40-41 0.2462752261519583 1103 42-43 0.2721408041872735 1103 44-45 0.3971204523634668 1103 46-47 0.7837020120198943 1103 48-49 0.5940653550535515 1103 50-51 0.7344390968351817 1103 52-53 0.7525445861307887 1103 54-55 0.8054009143050109 1103 56-57 0.5580250922949332 1103 58-59 0.47028045981662814 1103 60-61 0.4187003378930214 1103 62-63 0.5272023849200238 1103 64-65 0.5213802598512984 1103 66-67 0.5891571387541923 1103 68-69 0.6403697707544538 1103 70-71 0.7165481898674955 1103 72-73 0.4862535395109937 1103 74-75 0.38550728365063236 1103 76-77 0.5137892472022116 1103 78-79 0.46155941972524417 1103 80-81 0.585872401748933 1103 82-83 0.3861956231845518 1103 84-85 0.5970465436504284 1103 86-87 0.5649167019144201 1103 88-89 0.2641294764290194 1103 90-91 0.31674970429572014 1103 92-93 0.12676055192097024 1103 94-95 0.3046016439496668 1103 96-97 0.4502456571224194 1103 98-99 0.5023164640342195 1103 100-101 0.39609758825953634 1104 1 0.1440836251108344 1104 2 0.12144587195395218 1104 3 0.14883260070291016 1104 4 0.3460601440164126 1104 5 0.32389088133798083 1104 6 0.3529485978688527 1104 7 0.3012833001008275 1104 8 0.35926109875858714 1104 9 0.2766029458726962 1104 10-11 0.4103190860339012 1104 12-13 0.3817597171834777 1104 14-15 0.2848676793002056 1104 16-17 0.33907363893477793 1104 18-19 0.24731057420011382 1104 20-21 0.057939480627688056 1104 22-23 0.2553102588353866 1104 24-25 0.20078289474066224 1104 26-27 0.14296257065584683 1104 28-29 0.06085418920421404 1104 30-31 0.2022732293129863 1104 32-33 0.2310919988202471 1104 34-35 0.3769803979257986 1104 36-37 0.40122859283990664 1104 38-39 0.2826380069466339 1104 40-41 -0.013470397413897217 1104 42-43 0.313140735267055 1104 44-45 0.35225034671282174 1104 46-47 0.2818487674990493 1104 48-49 0.6708551524281035 1104 50-51 0.5266674295333686 1104 52-53 0.5459126363371425 1104 54-55 0.5233595986584945 1104 56-57 0.4891297010307767 1104 58-59 0.5527901289184811 1104 60-61 0.5611052279855286 1104 62-63 0.664601437064249 1104 64-65 0.6914039157042495 1104 66-67 1.008181958142675 1104 68-69 0.6678197833222583 1104 70-71 0.7413260235021824 1104 72-73 0.5759293914865715 1104 74-75 0.5602251161501677 1104 76-77 0.46047083982693593 1104 78-79 0.7514535866419187 1104 80-81 0.7341578084998694 1104 82-83 0.5162267994500489 1104 84-85 0.3400359380443767 1104 86-87 0.38131069450346544 1104 88-89 0.26822364172403823 1104 90-91 0.11241898047121168 1104 92-93 0.021032369225618908 1104 94-95 0.1877504467648592 1104 96-97 0.3177916309651678 1104 98-99 0.4600359344025762 1104 100-101 0.5133197384083239 1105 1 0.1450614845792586 1105 2 0.10624450014880793 1105 3 0.33258892596867184 1105 4 0.17734099982556728 1105 5 0.19908463200342652 1105 6 0.1900248821149546 1105 7 0.35808432671317547 1105 8 0.3640545714765864 1105 9 0.4626360866228225 1105 10-11 0.34174928255843895 1105 12-13 0.3086830742955158 1105 14-15 0.2060185834699837 1105 16-17 0.31115252893819445 1105 18-19 0.28717557915945235 1105 20-21 0.23237952299225384 1105 22-23 -0.06855931880777888 1105 24-25 -0.003381273574440513 1105 26-27 -0.18357048323569813 1105 28-29 -0.18120469530750682 1105 30-31 -0.20943614749072736 1105 32-33 -0.2437820562424804 1105 34-35 -0.22928294607338984 1105 36-37 0.12614411586863383 1105 38-39 -0.15801488936664043 1105 40-41 -0.1215331149105694 1105 42-43 -0.3022948745081777 1105 44-45 -0.15792527572695292 1105 46-47 0.006069141719429183 1105 48-49 -0.014795810581354374 1105 50-51 -0.001759701723948126 1105 52-53 0.16919381716549253 1105 54-55 0.049395597336008734 1105 56-57 0.15212654610309073 1105 58-59 0.13918751531586082 1105 60-61 0.17733825553931837 1105 62-63 0.15097640834488146 1105 64-65 0.08236389911706254 1105 66-67 0.3231199523259498 1105 68-69 0.26568334250657344 1105 70-71 0.31016620932350136 1105 72-73 0.16756406991370554 1105 74-75 0.3197240822529075 1105 76-77 0.0714144602045188 1105 78-79 0.18573302469494024 1105 80-81 0.2794761311766827 1105 82-83 0.3412230168990966 1105 84-85 0.4255106621983451 1105 86-87 0.30851396038597656 1105 88-89 0.03794502182277526 1105 90-91 -0.042964009817438864 1105 92-93 -0.23654562137123847 1105 94-95 0.06758076433102289 1105 96-97 0.039506835699242515 1105 98-99 0.042285403444498826 1105 100-101 -0.19434427019908185 1106 1 0.04232806934584232 1106 2 0.0705038894081973 1106 3 0.08544080382054631 1106 4 -0.010698663214093074 1106 5 0.05908375450255221 1106 6 0.17836728295735327 1106 7 0.25199885812770617 1106 8 0.28940995933197655 1106 9 0.25084686233359577 1106 10-11 0.3424600582692179 1106 12-13 0.28384760571005074 1106 14-15 0.15733483478622645 1106 16-17 0.32947474726042003 1106 18-19 0.026979018962897783 1106 20-21 0.03444823131096797 1106 22-23 0.05908195883349521 1106 24-25 0.10419375275058229 1106 26-27 0.0026812717660575913 1106 28-29 0.08161998001717308 1106 30-31 0.22542696237237436 1106 32-33 0.06536150290106946 1106 34-35 0.025087008296559077 1106 36-37 -0.03470398872946845 1106 38-39 0.0023440084922583537 1106 40-41 -0.049027411154867195 1106 42-43 -0.16933374779705446 1106 44-45 -0.14887824167992392 1106 46-47 -0.005270363370080133 1106 48-49 0.08464478260923158 1106 50-51 -0.04226952973377962 1106 52-53 0.008861244332919682 1106 54-55 0.18003134672175491 1106 56-57 0.09828528601182995 1106 58-59 0.18974692212527344 1106 60-61 0.3169354826365449 1106 62-63 0.44502914614761835 1106 64-65 0.502899613402775 1106 66-67 0.3222205139265135 1106 68-69 0.3218455924188248 1106 70-71 0.1256957811030759 1106 72-73 -0.025205322855686774 1106 74-75 0.22502199380081933 1106 76-77 0.16164348793354577 1106 78-79 0.06946635217826724 1106 80-81 0.13040189460244633 1106 82-83 0.034578141504223225 1106 84-85 0.150014874202558 1106 86-87 0.36452264764466236 1106 88-89 0.3316401780179312 1106 90-91 0.2148896230361963 1106 92-93 0.05103774121212368 1106 94-95 0.46615482540508424 1106 96-97 0.30636143380383984 1106 98-99 0.4119107105698063 1106 100-101 0.24248444162963168 1107 1 0.23276599163860467 1107 2 0.15898664321353095 1107 3 0.10316098686331188 1107 4 0.3066466259053868 1107 5 0.2942390561901078 1107 6 0.25253035107203203 1107 7 0.3817958084891728 1107 8 0.38881928656388 1107 9 0.4879424280581972 1107 10-11 0.48110501546256046 1107 12-13 0.4265573824924047 1107 14-15 0.22495538627774891 1107 16-17 0.22822653310898033 1107 18-19 0.16383678485291853 1107 20-21 0.23265895875556453 1107 22-23 0.40257448780989336 1107 24-25 0.3897586582751629 1107 26-27 0.27943509589891136 1107 28-29 0.3943240769954599 1107 30-31 0.38748399136489553 1107 32-33 0.3171876337594597 1107 34-35 0.3318393956941108 1107 36-37 0.3846001614214529 1107 38-39 0.18002318415530283 1107 40-41 0.44846602283050174 1107 42-43 0.29396068626350313 1107 44-45 0.3583094630884318 1107 46-47 0.39661303548913196 1107 48-49 0.3972780498796027 1107 50-51 0.15226241681430253 1107 52-53 0.36118566722186074 1107 54-55 0.293413596192714 1107 56-57 0.32412899875070167 1107 58-59 0.3307057616728244 1107 60-61 0.43424223502393744 1107 62-63 0.6171819261633047 1107 64-65 0.44871152998082664 1107 66-67 0.4836645806125155 1107 68-69 0.41585339912824537 1107 70-71 0.3806427830258805 1107 72-73 0.4644931507137535 1107 74-75 0.6953477030378146 1107 76-77 0.5436129969191832 1107 78-79 0.24997099538452616 1107 80-81 0.08259016332297264 1107 82-83 0.12134251959924569 1107 84-85 0.12343325689513307 1107 86-87 0.2159900646362729 1107 88-89 0.38077182593990244 1107 90-91 0.20677811089242581 1107 92-93 0.2701506175830666 1107 94-95 0.22916936462929982 1107 96-97 0.24588595498158838 1107 98-99 0.4122886928026297 1107 100-101 0.2366018833277188 1108 1 0.06128239612290187 1108 2 0.11687110965287317 1108 3 0.08613480112352434 1108 4 0.16379534137813323 1108 5 0.15841876233849206 1108 6 0.18959652378200786 1108 7 0.30799168429125245 1108 8 0.17875383575489678 1108 9 0.24705265607212823 1108 10-11 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599.0 4 588.0 5 631.0 6 700.0 7 828.0 8 1066.0 9 1272.0 10 1721.0 11 2478.0 12 3527.0 13 4888.0 14 5838.0 15 7928.0 16 11250.0 17 15943.0 18 11964.0 19 7015.0 20 6620.0 21 6592.0 22 7190.0 23 8181.0 24 9060.0 25 10300.0 26 11590.0 27 13847.0 28 16393.0 29 19855.0 30 24711.0 31 30790.0 32 39260.0 33 51763.0 34 70330.0 35 99235.0 36 133304.0 37 162454.0 38 118969.0 39 18782.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.05683327351519 18.01520197410531 14.620584329207833 19.30738042317167 2 15.35082207449471 22.03210765885027 37.88752734390808 24.729542922746937 3 22.609775501922915 25.99189084700923 30.495697803584687 20.902635847483168 4 11.781790993270834 16.04167864879891 39.524847214500404 32.65168314342985 5 11.73452213558311 39.81347735310409 33.55351740593975 14.898483105373044 6 28.649123741543708 36.327811218238956 17.199062483353753 17.82400255686358 7 25.88688584646055 35.66094720430748 20.555666783100605 17.896500166131354 8 26.290153188715458 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22.740049294945962 32-33 20.581980550597837 25.60459910633277 32.10471837555321 21.708701967516177 34-35 22.7946125077737 24.827807263402708 31.15889864801547 21.218681580808123 36-37 21.76349096733075 24.055827960515984 33.45013409636303 20.730546975790244 38-39 21.366435088556265 25.644578005332967 32.23310658272492 20.75588032338584 40-41 20.68345304588123 26.698169968745173 31.932421423665282 20.68595556170831 42-43 20.148992270846243 26.973873373075964 29.571414271140164 23.30572008493763 44-45 20.98639747362637 25.2912107776607 30.122671391992046 23.59972035672089 46-47 23.03477784445198 26.718992013826586 29.737807095495977 20.508423046225456 48-49 22.279117663345588 26.33304651925559 29.40518735916648 21.982648458232347 50-51 19.82721577794166 27.328074374422613 31.587085644000606 21.257624203635125 52-53 21.677779913518474 28.447160917294124 27.92743048918342 21.947628680003984 54-55 20.041713301719117 26.431611803256345 29.630980512848907 23.89569438217563 56-57 22.38644646809607 26.066077674358922 28.253101633918543 23.29437422362646 58-59 24.136886299921994 25.80723141761024 27.886031026780365 22.1698512556874 60-61 21.905316672452678 26.31969040920253 31.152573995310135 20.622418923034655 62-63 21.17805101806648 26.969590318824316 30.207564057442582 21.644794605666625 64-65 20.303484981758317 29.171307842540077 29.08994971556603 21.43525746013558 66-67 21.28777405962907 26.955613682692576 27.775582626328895 23.98102963134946 68-69 20.5606038009204 27.01066345662178 29.102501278336458 23.326231464121356 70-71 22.83696345235692 26.850274036743137 27.810321509101087 22.502441001798857 72-73 21.841666502475906 25.684901781088097 30.5129621060154 21.960469610420592 74-75 20.288735871173973 29.543100275719482 28.427280517545228 21.740883335561314 76-77 21.025151062952748 26.95013138456 28.200707889570932 23.82400966291632 78-79 21.311435237963327 27.107895886382767 29.09390681767332 22.486762057980588 80-81 22.73513190362039 27.03519365809759 27.91105223981198 22.318622198470045 82-83 21.55693152109297 26.34016368118604 29.975209182510277 22.127695615210715 84-85 21.175171846742742 27.618652534087108 28.75943373116715 22.446741888003 86-87 21.117471948706207 28.291733455461415 28.198593041894547 22.39220155393783 88-89 20.7043388375837 27.9195808199233 26.75787813252059 24.61820220997241 90-91 21.107062051804288 26.406636811807864 27.801129996463438 24.68517113992441 92-93 22.55109708486065 27.776076568274032 28.156052805779687 21.51677354108563 94-95 21.571274298056156 27.002366437903902 28.226960778048998 23.199398485990944 96-97 19.23792361023465 27.39819694890397 30.229410001081042 23.134469439780343 98-99 20.968247152919318 28.63866446905102 26.756911675241557 23.63617670278811 100-101 20.52447140726643 26.93114907479288 27.860272385835 24.684107132105694 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 419.0 1 369.0 2 491.0 3 685.0 4 595.0 5 547.5 6 947.0 7 1993.0 8 3229.0 9 3778.0 10 3201.5 11 2425.5 12 2305.5 13 2386.5 14 2473.0 15 2556.5 16 2565.5 17 2594.5 18 2684.5 19 2792.0 20 3003.0 21 3283.0 22 3600.0 23 4109.5 24 4638.0 25 5313.0 26 6316.5 27 7427.5 28 8383.5 29 9569.0 30 11183.0 31 12912.5 32 15201.0 33 16439.0 34 16851.0 35 17496.0 36 18527.5 37 19934.0 38 20386.0 39 20228.0 40 20429.5 41 21604.0 42 22445.0 43 22965.0 44 23816.0 45 25713.5 46 29004.5 47 45726.5 48 82303.0 49 79134.0 50 42622.0 51 27664.0 52 23347.0 53 22500.0 54 22270.5 55 20930.5 56 19320.5 57 17906.5 58 17418.0 59 16145.0 60 13940.5 61 12325.0 62 10899.0 63 8417.5 64 5976.0 65 4805.5 66 3618.0 67 2731.0 68 2028.5 69 1433.5 70 1172.5 71 942.5 72 762.5 73 763.5 74 662.5 75 505.0 76 392.0 77 264.5 78 173.0 79 117.0 80 70.0 81 50.5 82 43.5 83 28.5 84 11.0 85 6.0 86 5.0 87 4.5 88 3.5 89 3.5 90 3.5 91 3.5 92 3.0 93 2.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011394324561478238 2 0.010435923430138947 3 0.013311126824156819 4 0.01767717642248026 5 0.032372660436349385 6 0.04408645204160738 7 0.005111472700476219 8 0.023640561239702512 9 0.033544039596875186 10-11 0.04142422667677602 12-13 0.030935058739340448 14-15 0.027154698721279916 16-17 0.026888476184796773 18-19 0.02214971503539695 20-21 0.048558990654524084 22-23 0.010702145966622084 24-25 0.002502491842941482 26-27 9.051566240426638E-4 28-29 0.0 30-31 0.0023960028283482276 32-33 0.0012778681751190548 34-35 0.0 36-37 5.3244507296627286E-5 38-39 0.0 40-41 9.58401131339291E-4 42-43 0.0023427583210516006 44-45 0.0011181346532291728 46-47 3.7271155107639097E-4 48-49 0.0012778681751190548 50-51 0.0033544039596875184 52-53 0.002076535784568464 54-55 0.00372711551076391 56-57 2.662225364831364E-4 58-59 0.003886849032653791 60-61 0.001916802262678582 62-63 4.792005656696455E-4 64-65 6.389340875595274E-4 66-67 0.013417615838750074 68-69 0.03663222102007957 70-71 0.04392671851971751 72-73 0.0122994811855209 74-75 0.0263560311118305 76-77 0.02310811616673624 78-79 0.050475792917202666 80-81 0.07140088428477719 82-83 0.04366049598323437 84-85 0.015547396130615164 86-87 0.016931753320327474 88-89 0.01954073417786221 90-91 0.03194670437797637 92-93 0.020179668265421738 94-95 0.010648901459325457 96-97 0.016559041769251083 98-99 0.05015632587342289 100-101 0.0694840820220986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 939064.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.483609733146665 #Duplication Level Percentage of deduplicated Percentage of total 1 76.54116964920792 34.81368688843144 2 12.831674537553377 11.672617537776663 3 4.479431981537689 6.112222082233083 4 2.0155059249807357 3.666899396266742 5 1.1224354604990807 2.552620821799248 6 0.6691539938343372 1.8261323464162451 7 0.4177676124548647 1.3301105330831868 8 0.30182430896442314 1.09824472615316 9 0.22416554487251628 0.9176272342739736 >10 1.2221553023857261 10.273172421627448 >50 0.10440667021257242 3.2451071415063564 >100 0.0573759173723488 5.069485903278959 >500 0.004938091247495814 1.4625882342248635 >1k 0.005878680056542636 6.628225275291953 >5k 0.0014108832135702326 4.566697800726378 >10k+ 7.054416067851163E-4 4.764561656910363 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18821 2.004229743659644 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14826 1.5788061303595922 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10901 1.160836748081068 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9090 0.967985142652684 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8894 0.9471132957924061 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7351 0.7828007462750144 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6565 0.6991003808047163 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5399 0.5749341897889814 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5399 0.5749341897889814 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4874 0.5190274571275227 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4513 0.48058492285935783 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4444 0.4732371808524233 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3970 0.4227613879352206 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3949 0.42052511862876224 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3088 0.3288380770639701 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3067 0.32660180775751174 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3053 0.3251109615532062 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2914 0.31030898852474376 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2617 0.2786817511905472 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2413 0.2569579922135233 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2342 0.24939727217740218 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2317 0.2467350468125708 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2232 0.23768348057214417 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1939 0.2064821992963206 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1835 0.19540734177862212 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1708 0.1818832369252788 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1675 0.17836909944370138 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1665 0.17730420929776883 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1649 0.17560038506427678 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1294 0.1377967848836714 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1245 0.13257882316860192 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1133 0.12065205353415742 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1025 0.10915123995808593 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1012 0.10776688276837362 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 940 0.1000996737176593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 3.194670437797637E-4 0.0 26-27 0.0 0.0 0.0 7.454231021527819E-4 0.0 28-29 0.0 0.0 0.0 0.0014376016970089364 0.0 30-31 0.0 0.0 0.0 0.0034608929742807734 0.0 32-33 0.0 0.0 0.0 0.006602318904781783 0.0 34-35 0.0 0.0 0.0 0.011234591039588356 0.0 36-37 0.0 0.0 0.0 0.016292819232767947 0.0 38-39 0.0 0.0 0.0 0.026249542097237247 0.0 40-41 0.0 0.0 0.0 0.041051515125699635 0.0 42-43 0.0 0.0 0.0 0.06277527410272356 0.0 44-45 0.0 0.0 0.0 0.08386009899218796 0.0 46-47 0.0 0.0 0.0 0.12299481185520902 0.0 48-49 0.0 0.0 0.0 0.16867859911571523 0.0 50-51 0.0 0.0 0.0 0.19785658911426696 0.0 52-53 0.0 0.0 0.0 0.2375769915575509 0.0 54-55 0.0 0.0 0.0 0.29619919409113754 0.0 56-57 0.0 0.0 0.0 0.3899095269332016 0.0 58-59 0.0 0.0 0.0 0.496132318989973 0.0 60-61 0.0 0.0 0.0 0.6266878508813031 0.0 62-63 0.0 0.0 0.0 0.7620886329366263 0.0 64-65 0.0 0.0 0.0 0.887266469590997 0.0 66-67 0.0 0.0 0.0 1.053549065878364 0.0 68-69 0.0 0.0 0.0 1.2561976606493275 0.0 70-71 0.0 0.0 0.0 1.4264203504766448 0.0 72-73 0.0 0.0 0.0 1.6154383513796717 0.0 74-75 0.0 0.0 0.0 1.8127092509136757 0.0 76-77 0.0 0.0 0.0 2.031384442380924 0.0 78-79 0.0 0.0 0.0 2.3086818363817585 0.0 80-81 0.0 0.0 0.0 2.6532270430982337 0.0 82-83 0.0 0.0 0.0 2.9918088649974868 0.0 84-85 0.0 0.0 0.0 3.401099392586661 0.0 86-87 0.0 0.0 0.0 3.849205165995076 0.0 88-89 0.0 0.0 0.0 4.367646933542336 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 140 4.5656634E-10 33.927658 2 TAAGGTG 330 0.0 30.236126 5 AAGGGTA 145 2.3934263E-8 29.48982 4 GTACATG 8180 0.0 27.870623 1 TACATGG 8120 0.0 27.668589 2 ACATGGG 8235 0.0 26.248177 3 GGTAAGG 295 0.0 25.766138 3 CATGGGA 5710 0.0 25.627853 4 GTAAGGT 300 0.0 25.339403 4 ATGGGAG 1935 0.0 25.046091 5 TAAGGGT 155 1.4085435E-6 24.519386 3 TAAGGCG 375 0.0 24.073713 5 TATCGTA 120 1.7052483E-4 23.760754 7 TTTGCGT 200 2.050183E-8 23.753157 3 ATGGGAT 1910 0.0 23.632572 5 GAGTACT 6745 0.0 23.600973 12-13 CATGGGT 730 0.0 22.128588 4 GTACTTT 7165 0.0 22.017975 14-15 AGTACTT 6860 0.0 21.854572 12-13 GTAAGCG 100 5.3567965E-7 21.388668 94-95 >>END_MODULE