##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936383_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 939064 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820645877171312 31.0 31.0 34.0 30.0 34.0 2 31.92415745891654 31.0 31.0 34.0 30.0 34.0 3 32.0033245870356 33.0 31.0 34.0 30.0 34.0 4 35.7380625814641 37.0 35.0 37.0 35.0 37.0 5 35.62752165986557 37.0 35.0 37.0 33.0 37.0 6 35.742824770196705 37.0 35.0 37.0 33.0 37.0 7 35.67445988771798 37.0 35.0 37.0 33.0 37.0 8 35.66047255565116 37.0 35.0 37.0 33.0 37.0 9 37.21169377167051 39.0 37.0 39.0 33.0 39.0 10-11 37.09233768944395 39.0 37.0 39.0 33.0 39.0 12-13 36.97574233492073 39.0 37.0 39.0 33.0 39.0 14-15 37.851121435812686 39.5 37.0 41.0 33.0 41.0 16-17 37.90358537863234 39.5 37.0 41.0 32.5 41.0 18-19 38.18895730216471 40.0 37.5 41.0 33.0 41.0 20-21 38.22229102595776 40.0 37.5 41.0 33.5 41.0 22-23 38.116923340688174 40.0 37.0 41.0 33.0 41.0 24-25 37.92208731247284 40.0 37.0 41.0 33.0 41.0 26-27 37.635078120341106 39.5 36.5 41.0 32.5 41.0 28-29 37.64899144254279 39.0 37.0 41.0 32.5 41.0 30-31 37.497884595725104 39.0 36.5 41.0 32.5 41.0 32-33 37.18966492166668 39.0 35.5 41.0 32.0 41.0 34-35 36.880835065554635 39.0 35.0 41.0 31.0 41.0 36-37 36.59893787856845 38.5 35.0 40.0 30.5 41.0 38-39 36.345046237530134 38.0 35.0 40.0 30.0 41.0 40-41 36.142112784645136 38.0 35.0 40.0 30.0 41.0 42-43 35.773157101113455 38.0 35.0 40.0 29.5 41.0 44-45 35.32684832982629 38.0 35.0 40.0 27.5 41.0 46-47 34.71412704565397 38.0 34.0 40.0 25.0 41.0 48-49 34.08319826976649 38.0 33.0 40.0 23.0 41.0 50-51 33.66278549704813 38.0 33.0 40.0 20.5 41.0 52-53 33.44342558121704 37.5 33.0 40.0 20.0 41.0 54-55 33.1275269843163 37.0 32.5 40.0 18.0 41.0 56-57 32.692261123842464 36.5 31.5 39.5 15.5 41.0 58-59 32.307433252685655 36.0 31.0 39.0 12.5 40.0 60-61 31.750521263726434 35.5 31.0 39.0 8.0 40.0 62-63 31.485340189806017 35.0 31.0 39.0 8.0 40.0 64-65 31.17530807271922 35.0 30.5 38.0 6.5 40.0 66-67 30.667185090686043 35.0 29.5 38.0 2.0 40.0 68-69 30.141592585808848 34.0 29.0 37.0 2.0 39.0 70-71 29.50502415170851 34.0 28.5 36.5 2.0 39.0 72-73 28.8786318078427 34.0 27.0 36.0 2.0 38.5 74-75 28.377615370198413 33.0 26.0 35.0 2.0 37.5 76-77 27.101727358305716 31.5 25.5 34.0 2.0 36.0 78-79 27.89042067420325 33.0 26.0 35.0 2.0 36.0 80-81 27.64188543059898 33.0 26.0 35.0 2.0 36.0 82-83 27.17537675813363 33.0 25.5 35.0 2.0 36.0 84-85 26.721212824685004 32.0 25.0 35.0 2.0 35.5 86-87 26.367547898758765 32.0 25.0 34.0 2.0 35.0 88-89 25.802779150302854 32.0 23.5 34.0 2.0 35.0 90-91 25.100258874794477 31.0 20.0 34.0 2.0 35.0 92-93 24.473766963700022 31.0 18.0 34.0 2.0 35.0 94-95 23.6352437107588 31.0 6.0 34.0 2.0 35.0 96-97 22.69071809802101 30.0 2.0 34.0 2.0 35.0 98-99 21.523748115144443 29.5 2.0 34.0 2.0 35.0 100-101 17.901584982493205 23.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2798803021235692 1101 2 0.3532914700898111 1101 3 0.3279114555168583 1101 4 0.23503029662462183 1101 5 0.22431235173009156 1101 6 0.19015551001061937 1101 7 0.19931491573191096 1101 8 0.257567808932734 1101 9 0.24295088607875215 1101 10-11 0.36909230626429235 1101 12-13 0.31124424391017413 1101 14-15 0.3913020262996767 1101 16-17 0.3896724279416546 1101 18-19 0.346420189293859 1101 20-21 0.28967799927571036 1101 22-23 0.194798387640013 1101 24-25 0.1888770540478788 1101 26-27 0.20224728506727985 1101 28-29 0.27523944435884573 1101 30-31 0.2833507491202738 1101 32-33 0.32501772865944645 1101 34-35 0.37091033438735366 1101 36-37 0.3941820334852011 1101 38-39 0.17836080696353207 1101 40-41 0.16755025708324922 1101 42-43 0.36996196290831307 1101 44-45 0.41149899071270823 1101 46-47 0.31097459853410925 1101 48-49 0.5454348682647279 1101 50-51 0.6827145262531715 1101 52-53 0.56972176885197 1101 54-55 0.5709655517812493 1101 56-57 0.526241572997975 1101 58-59 0.5742910644396275 1101 60-61 0.7134912811730345 1101 62-63 0.5083670030895 1101 64-65 0.3449871983966908 1101 66-67 0.5456852475311749 1101 68-69 0.4853323078209826 1101 70-71 0.5547326173661808 1101 72-73 0.8678894394027203 1101 74-75 0.9380034760535594 1101 76-77 0.6936564602129103 1101 78-79 0.6949157146376805 1101 80-81 0.7218153265406997 1101 82-83 0.7787844379791231 1101 84-85 0.8223610108097716 1101 86-87 0.9875350006020547 1101 88-89 0.7365887973486593 1101 90-91 0.8331680099576104 1101 92-93 0.6635731508756422 1101 94-95 0.5132871376925721 1101 96-97 0.6147449033135182 1101 98-99 0.44506536712176725 1101 100-101 0.5476903416844543 1102 1 0.202103651401778 1102 2 0.28492514367541943 1102 3 0.2949922262807867 1102 4 0.20193355607053576 1102 5 0.04968081005986846 1102 6 0.015014568693459296 1102 7 0.12208058655563292 1102 8 0.09748908110608312 1102 9 0.297292038807889 1102 10-11 0.21097176406732387 1102 12-13 0.23566134834911878 1102 14-15 0.4872255389416864 1102 16-17 0.35665309833827763 1102 18-19 0.34699943675263967 1102 20-21 0.1997787491831673 1102 22-23 0.2040051745338971 1102 24-25 0.2983096673703969 1102 26-27 0.3032667961859872 1102 28-29 0.26885949183755997 1102 30-31 0.3475428902181932 1102 32-33 0.45423039700602175 1102 34-35 0.5317453761002824 1102 36-37 0.5344822634046196 1102 38-39 0.32913198019321754 1102 40-41 0.42792710604825146 1102 42-43 0.2501060095613141 1102 44-45 0.49817278452552927 1102 46-47 0.3612029608387175 1102 48-49 0.47795120464467544 1102 50-51 0.6126807501647917 1102 52-53 0.6545334733877226 1102 54-55 0.6658813873924245 1102 56-57 0.690119888322144 1102 58-59 0.5506264478284848 1102 60-61 0.49142551211748753 1102 62-63 0.45545750805803564 1102 64-65 0.3713656471880107 1102 66-67 0.2943877243256878 1102 68-69 0.29731045123945066 1102 70-71 0.3157703512006371 1102 72-73 0.3465871914598928 1102 74-75 0.46165051948911184 1102 76-77 0.32408345723748155 1102 78-79 0.1359129990259369 1102 80-81 0.5599234473943184 1102 82-83 0.5657897642084464 1102 84-85 0.30292374393240706 1102 86-87 0.6861803979426817 1102 88-89 0.5706699916483657 1102 90-91 0.6018502976202598 1102 92-93 0.5283798192506914 1102 94-95 0.8408053070214621 1102 96-97 0.704090042261722 1102 98-99 0.49664441704916484 1102 100-101 0.1925464729876616 1103 1 0.2879899156718757 1103 2 0.3415425228349953 1103 3 0.25206807034199485 1103 4 0.170679760831284 1103 5 0.14812552101444965 1103 6 0.17216783563540616 1103 7 0.25885721395422934 1103 8 0.1973797782156268 1103 9 0.12390653358563952 1103 10-11 0.25305527451182286 1103 12-13 0.27861966144116934 1103 14-15 0.37878227017802857 1103 16-17 0.28060788944831216 1103 18-19 0.4228359074837513 1103 20-21 0.4455755217117101 1103 22-23 0.3551904277522553 1103 24-25 0.24700372554382 1103 26-27 0.4409353048087681 1103 28-29 0.4128451761951055 1103 30-31 0.48125106668164364 1103 32-33 0.3696990331488408 1103 34-35 0.48878326776898007 1103 36-37 0.4865142345000777 1103 38-39 0.29699768944752947 1103 40-41 0.45841616057825263 1103 42-43 0.6469343281185118 1103 44-45 0.7890759755626888 1103 46-47 0.928946083865192 1103 48-49 0.9702772308773646 1103 50-51 0.9446047846559296 1103 52-53 0.8490205088504368 1103 54-55 0.7234608597023566 1103 56-57 0.6861481704450512 1103 58-59 0.6694629792834306 1103 60-61 0.3199072646071599 1103 62-63 0.6496333339020985 1103 64-65 0.7673305488154121 1103 66-67 0.32128268728414966 1103 68-69 0.4065108702338627 1103 70-71 0.4289519761702749 1103 72-73 0.6539939803838699 1103 74-75 0.666219608024452 1103 76-77 0.4618942873322105 1103 78-79 0.21826589555253761 1103 80-81 0.3857124776347831 1103 82-83 0.3868954692120852 1103 84-85 0.4657319173596619 1103 86-87 0.5229092053266982 1103 88-89 0.42828400063548955 1103 90-91 0.5483791718004909 1103 92-93 0.6851839241814623 1103 94-95 0.7765253398123271 1103 96-97 0.9226616660168858 1103 98-99 0.7808679870030097 1103 100-101 0.7806662355574936 1104 1 0.18706334408809155 1104 2 0.21495330357950237 1104 3 0.08385407710428794 1104 4 0.12488328630338685 1104 5 0.00808875004850762 1104 6 0.11799147283934985 1104 7 0.08084742728258476 1104 8 0.045894142000889815 1104 9 0.21766438846398017 1104 10-11 0.21503158217169016 1104 12-13 0.11553313007436117 1104 14-15 0.2462724997229131 1104 16-17 0.03699973334806117 1104 18-19 0.06255088182110313 1104 20-21 0.2577288935322599 1104 22-23 0.1868198437141828 1104 24-25 0.016738727287098243 1104 26-27 0.28371459387640385 1104 28-29 0.2261458670189853 1104 30-31 0.24504271273981004 1104 32-33 0.37530082831793976 1104 34-35 0.36106243926681714 1104 36-37 0.5040417620478976 1104 38-39 0.4344742768424297 1104 40-41 0.4688028435695486 1104 42-43 0.5293924084065154 1104 44-45 0.6079495758740521 1104 46-47 0.5297763698435745 1104 48-49 0.4548021070236743 1104 50-51 0.5414113458611425 1104 52-53 0.5784179231258406 1104 54-55 0.25281276636004435 1104 56-57 0.57281899935262 1104 58-59 0.6368809439476237 1104 60-61 0.46548704584626677 1104 62-63 0.24064654226009452 1104 64-65 0.36298472335385057 1104 66-67 0.5150204742152553 1104 68-69 0.3593795731552696 1104 70-71 0.38186527452997154 1104 72-73 0.4428573845563797 1104 74-75 0.5592128538426167 1104 76-77 0.48829681386649426 1104 78-79 0.6758505660733292 1104 80-81 0.7046043215345321 1104 82-83 0.7356627094031154 1104 84-85 0.7453635590393688 1104 86-87 0.6148340863379609 1104 88-89 0.6128635061937047 1104 90-91 0.8912435976139506 1104 92-93 1.090718116720243 1104 94-95 1.064216086664299 1104 96-97 1.1030466665439214 1104 98-99 0.9316365488406682 1104 100-101 0.9505403953388942 1105 1 0.12264566080248329 1105 2 0.2208608076002072 1105 3 0.1782021054145808 1105 4 0.1995839044002352 1105 5 0.12960726213305662 1105 6 0.20350290174700802 1105 7 0.13686728707602924 1105 8 0.1863635145457323 1105 9 0.20690625317754296 1105 10-11 0.09844579671798215 1105 12-13 0.115514357414078 1105 14-15 0.2752860664723258 1105 16-17 0.28191906694664226 1105 18-19 0.2489939390188809 1105 20-21 0.17110371351085263 1105 22-23 0.14070438344777614 1105 24-25 0.18593111063042755 1105 26-27 0.24061256586870883 1105 28-29 0.20123762890320052 1105 30-31 0.1687052307419421 1105 32-33 0.22736442000417156 1105 34-35 0.1677135647858634 1105 36-37 0.2519790109285438 1105 38-39 0.08533109468037736 1105 40-41 0.3201544751098595 1105 42-43 0.48293791949920006 1105 44-45 0.35946595852249885 1105 46-47 0.1264914344831638 1105 48-49 0.4997354500702258 1105 50-51 0.4726574582393255 1105 52-53 0.43470648413138235 1105 54-55 0.43769264935313856 1105 56-57 0.4727996663144225 1105 58-59 0.5206994711623807 1105 60-61 0.5540760702843457 1105 62-63 0.49363475751246 1105 64-65 0.3710152191201921 1105 66-67 0.2853607917253811 1105 68-69 0.46409375239775486 1105 70-71 0.3768025402219557 1105 72-73 0.37680059585808223 1105 74-75 0.3907315900783317 1105 76-77 0.22766616455659872 1105 78-79 0.2747449404394011 1105 80-81 0.4211452767547357 1105 82-83 0.17406652893901509 1105 84-85 0.23442974527536364 1105 86-87 0.2997736495794108 1105 88-89 0.4271618289825909 1105 90-91 0.6604698838213707 1105 92-93 0.5207291561652418 1105 94-95 0.4982540018342867 1105 96-97 0.608135178236207 1105 98-99 0.5282938945546185 1105 100-101 0.24311959074827882 1106 1 0.08597928663610688 1106 2 0.19032816456757118 1106 3 0.1433653205777965 1106 4 0.15036488018120764 1106 5 0.11042509295089076 1106 6 -0.08298797224387044 1106 7 0.13765265036139596 1106 8 0.1642592674414871 1106 9 0.24231780108908652 1106 10-11 0.16436585638804502 1106 12-13 0.17242906432878868 1106 14-15 0.2889750801613431 1106 16-17 0.24757807010564647 1106 18-19 0.1161446936696322 1106 20-21 0.04262442253155996 1106 22-23 -0.0167872802938831 1106 24-25 -2.460567967403904E-4 1106 26-27 0.10103288878902816 1106 28-29 0.1898213424869084 1106 30-31 0.1318940689307837 1106 32-33 0.18654088543063807 1106 34-35 0.24146093228323906 1106 36-37 0.2608286072781354 1106 38-39 0.258841536940821 1106 40-41 0.3037079149132964 1106 42-43 0.4149184389797185 1106 44-45 0.5277508830574291 1106 46-47 0.4168433123350468 1106 48-49 0.3931827122674818 1106 50-51 0.44335551018738073 1106 52-53 0.42769305836795724 1106 54-55 0.452994070904559 1106 56-57 0.2655054402701893 1106 58-59 0.37736628407919426 1106 60-61 0.45312617558445467 1106 62-63 0.5714142415419445 1106 64-65 0.5204163247713005 1106 66-67 0.1296811672957574 1106 68-69 0.21675038630438692 1106 70-71 0.2398422032616203 1106 72-73 0.31390791421540243 1106 74-75 0.27671351356025653 1106 76-77 0.20928690867734545 1106 78-79 0.23378319947766002 1106 80-81 0.06250408686354447 1106 82-83 0.27718604455852613 1106 84-85 0.27885542095103943 1106 86-87 0.2898118208675804 1106 88-89 0.4912215867923493 1106 90-91 0.6773990695005523 1106 92-93 0.6117896159757024 1106 94-95 0.47733657466686097 1106 96-97 0.564453184554214 1106 98-99 0.7113756776364006 1106 100-101 0.5944336483123358 1107 1 0.10845479499871402 1107 2 0.22024381343483057 1107 3 0.05603598324845649 1107 4 0.11899973994510304 1107 5 0.17722120006957454 1107 6 0.1269962639500477 1107 7 -0.018236487382580435 1107 8 0.012613869828342672 1107 9 -0.031176849421690633 1107 10-11 0.09858184201532083 1107 12-13 0.19080584883460716 1107 14-15 0.2665996061729601 1107 16-17 0.25484701084232597 1107 18-19 0.26717578623298976 1107 20-21 0.14305826288475743 1107 22-23 0.24159378756299787 1107 24-25 0.330037570865926 1107 26-27 0.2715807549014144 1107 28-29 0.11305536652738368 1107 30-31 0.1823369056639379 1107 32-33 0.21167737528487152 1107 34-35 0.29905891226024295 1107 36-37 0.3634726030673008 1107 38-39 0.3076620041080602 1107 40-41 0.5059495576791377 1107 42-43 0.5936315269670018 1107 44-45 0.25700852058120205 1107 46-47 0.3273246320905585 1107 48-49 0.4117055201048103 1107 50-51 0.349899570965313 1107 52-53 0.3892197709027343 1107 54-55 0.31476167158344737 1107 56-57 0.21647819063810658 1107 58-59 0.287324941013658 1107 60-61 0.3091360930863054 1107 62-63 0.1127925540896122 1107 64-65 0.30742266520505623 1107 66-67 0.30542108597922635 1107 68-69 0.2635144421410196 1107 70-71 0.12040250317675927 1107 72-73 0.2189158766830417 1107 74-75 0.36132983809593355 1107 76-77 0.11122703844005599 1107 78-79 0.436934886500314 1107 80-81 0.5380429968782607 1107 82-83 0.5739276094452563 1107 84-85 0.4536715322590865 1107 86-87 0.5567750323872467 1107 88-89 0.4660206452607625 1107 90-91 0.49085812388702976 1107 92-93 0.18544009184391896 1107 94-95 0.6113312762018843 1107 96-97 1.023529054920406 1107 98-99 1.0240253935119235 1107 100-101 0.46597104908360976 1108 1 -1.9710712915355089E-4 1108 2 0.15308845152102535 1108 3 -0.10354282272885129 1108 4 0.02895787278261963 1108 5 0.08485501289062825 1108 6 -0.01909390326326843 1108 7 0.12103392063462337 1108 8 0.06899333131961072 1108 9 0.15919378058200095 1108 10-11 0.06391202467251844 1108 12-13 0.06267422608938489 1108 14-15 8.389650869133902E-5 1108 16-17 0.23553790026613086 1108 18-19 -0.01844902178235941 1108 20-21 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2316 78-79 -2.126292577118324 2316 80-81 -2.2458326225605987 2316 82-83 -2.1942935961873715 2316 84-85 -2.031847880580468 2316 86-87 -2.760780326440294 2316 88-89 -2.6725090696338256 2316 90-91 -2.8495029059405255 2316 92-93 -2.4477510740021593 2316 94-95 -2.4161660672430756 2316 96-97 -2.1647767243769245 2316 98-99 -1.4352817071580652 2316 100-101 -0.9531999022320221 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 22.0 5 50.0 6 114.0 7 230.0 8 564.0 9 1030.0 10 1756.0 11 2725.0 12 3923.0 13 5441.0 14 7107.0 15 9751.0 16 13154.0 17 17145.0 18 14548.0 19 9163.0 20 8202.0 21 8355.0 22 8943.0 23 9736.0 24 11166.0 25 13034.0 26 15523.0 27 18669.0 28 22845.0 29 27605.0 30 33515.0 31 40361.0 32 50079.0 33 62734.0 34 82082.0 35 107900.0 36 137549.0 37 137106.0 38 55231.0 39 1699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.3021316477536 19.004201524050927 15.234864661934406 18.45880216626107 2 16.03637951415883 24.356489419482635 36.08952172014612 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30.685435800393908 22.153388576549197 22-23 21.04658691332415 25.512319740336636 31.510805463324044 21.93028788301518 24-25 23.42757986160658 25.465357984882786 31.29841267136781 19.80864948214282 26-27 22.170291024137892 23.99791068330879 34.212134862091254 19.61966343046207 28-29 19.65607224644553 28.750824624745825 31.945177296317024 19.647925832491627 30-31 20.96705318835132 25.41299321980834 31.369062007818467 22.25089158402188 32-33 20.985473847761067 26.318687199191082 31.741886978112 20.95395197493585 34-35 23.099203938404393 24.92517787725072 31.64742846706895 20.328189717275936 36-37 22.43052309081596 24.218175118976244 33.38538932307335 19.96591246713444 38-39 21.218959461733263 26.13821519167978 32.85334403959899 19.78948130698797 40-41 20.818773171356355 26.9362508499159 31.45814952875924 20.78682644996851 42-43 20.338982148381362 27.56120170832861 29.48347073334494 22.616345409945087 44-45 22.04089717559971 25.231905611580665 29.860380069083135 22.866817143736483 46-47 23.76217169436801 27.271091214230342 29.34544397399964 19.621293117402008 48-49 23.093285994055613 27.11027361009204 28.90935074975746 20.887089646094882 50-51 20.138395260313754 28.02780897066662 31.347016318096117 20.486779450923514 52-53 22.314282808630587 29.181448806806415 27.428357535843194 21.075910848719804 54-55 20.383713092120743 27.274745730043097 28.828744884517622 23.512796293318537 56-57 23.150967530302886 26.424989110025486 28.246943085638332 22.177100274033297 58-59 25.13813316692216 26.052950914205418 27.34629373950723 21.462622179365194 60-61 21.778660251515664 27.291174801150554 30.89762062219723 20.032544325136552 62-63 21.24967321096648 27.237193730123106 30.09520675695894 21.41792630195147 64-65 20.555621522639814 30.054402433173717 28.501773922933527 20.888202121252945 66-67 22.08909178811868 26.895844115460875 27.463330117330848 23.551733979089594 68-69 21.157161736626488 27.693578221990776 28.226197207996684 22.923062833386044 70-71 23.602511592045204 27.530282021627123 27.45451040781422 21.412695978513447 72-73 22.666227914639304 25.99978488371474 29.999882857468425 21.334104344177536 74-75 20.711799370917472 30.661356686171736 27.53703061758609 21.089813325324698 76-77 21.92514735715541 27.195874532104426 27.39985836674494 23.47911974399523 78-79 22.017565532620942 27.969015908447087 27.95149723428591 22.061921324646057 80-81 23.17042001987163 27.43227830509666 27.700215222778475 21.697086452253238 82-83 22.315155000372723 26.761977785587256 29.338785768292812 21.58408144574721 84-85 21.11114305856394 28.472634876821274 28.479876299462642 21.936345765152144 86-87 21.279763251731925 28.77707571376163 27.024891473516238 22.918269560990208 88-89 20.84985730107344 28.696700886011246 25.97179033906969 24.48165147384563 90-91 22.179640374194868 26.59599393381726 26.880772245510006 24.343593446477865 92-93 23.099028315635284 28.44630071471967 27.26104705925576 21.193623910389288 94-95 21.996310130103854 28.00499436935438 27.228684079515897 22.770011421025867 96-97 19.394299843886774 28.29078378211449 29.54645167198758 22.76846470201116 98-99 21.324469812737274 29.483044124146083 25.697962313176543 23.4945237499401 100-101 20.30954068345586 28.027132065686477 26.466436608161665 25.196890642695998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1049.0 1 764.0 2 630.0 3 810.5 4 709.0 5 671.0 6 1067.0 7 2003.0 8 2997.0 9 3495.5 10 3164.5 11 2492.5 12 2357.5 13 2492.0 14 2594.0 15 2698.0 16 2755.5 17 2838.5 18 2905.5 19 3020.5 20 3268.5 21 3565.5 22 3907.0 23 4355.0 24 4937.5 25 5557.5 26 6509.5 27 7626.5 28 8511.0 29 9661.0 30 11218.5 31 12802.0 32 15034.5 33 16399.5 34 16640.5 35 17301.5 36 18574.0 37 19696.5 38 20279.5 39 20256.0 40 20245.5 41 21454.5 42 22320.0 43 22797.5 44 23590.5 45 25093.0 46 27527.0 47 45328.0 48 83996.5 49 79904.0 50 41991.0 51 26944.5 52 22520.5 53 21915.5 54 21913.5 55 20696.5 56 19129.5 57 17932.0 58 17708.5 59 16565.5 60 14130.0 61 12277.5 62 10796.0 63 8371.0 64 6008.5 65 4732.0 66 3517.0 67 2667.0 68 2071.0 69 1470.5 70 1125.0 71 912.5 72 831.0 73 806.5 74 649.0 75 550.5 76 459.0 77 303.5 78 211.5 79 160.0 80 112.5 81 89.5 82 62.5 83 38.5 84 25.5 85 12.5 86 4.0 87 1.5 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012672192736597294 2 0.007880187079900838 3 0.005217961715069473 4 1.0648901459325457E-4 5 0.00489849467128971 6 0.0 7 2.1297802918650915E-4 8 9.58401131339291E-4 9 1.0648901459325457E-4 10-11 0.0 12-13 0.0 14-15 6.389340875595274E-4 16-17 0.005217961715069473 18-19 0.001916802262678582 20-21 4.792005656696455E-4 22-23 2.1297802918650915E-4 24-25 2.1297802918650915E-4 26-27 1.0648901459325457E-4 28-29 5.3244507296627286E-5 30-31 5.324450729662728E-4 32-33 0.0035673819888740275 34-35 3.7271155107639097E-4 36-37 0.0011713791605258003 38-39 0.005324450729662728 40-41 5.3244507296627286E-5 42-43 0.001863557755381955 44-45 0.005377695236959356 46-47 0.0 48-49 0.002875203394017873 50-51 0.001544090711602191 52-53 9.051566240426638E-4 54-55 3.194670437797637E-4 56-57 0.01352410485334333 58-59 0.0026089808575347367 60-61 0.003940093539950419 62-63 2.662225364831364E-4 64-65 0.004632272134806574 66-67 0.0010648901459325455 68-69 0.002662225364831364 70-71 0.006176362846408764 72-73 0.003940093539950419 74-75 0.008252898630977229 76-77 0.0020232912772718365 78-79 0.007028274963154802 80-81 0.003940093539950419 82-83 0.0036206264961706554 84-85 0.0023427583210516006 86-87 7.986676094494091E-4 88-89 0.0025557363502381096 90-91 0.009371033284206402 92-93 0.0026089808575347367 94-95 6.921785948561547E-4 96-97 2.1297802918650915E-4 98-99 9.58401131339291E-4 100-101 0.0029284479013145003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 939064.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.736826910317525 #Duplication Level Percentage of deduplicated Percentage of total 1 75.60370536309122 33.06666175244166 2 13.210077893580873 11.555337806067174 3 4.7555673432280186 6.239802772533673 4 2.09620798155956 3.6672594262998617 5 1.1152882499000605 2.438958457049495 6 0.67915855397461 1.7822544071909439 7 0.47626007026981265 1.4581072980380523 8 0.33230072002833 1.1627023259242364 9 0.24117184896711197 0.9493282272524238 >10 1.3081485875074483 10.631699579473976 >50 0.10448368207149909 3.1990565317709185 >100 0.0624943543744705 5.124412576226824 >500 0.007323557151736363 2.2889291967141228 >1k 0.0046382528627663625 4.6160819521755005 >5k 0.002929422860694545 8.776257212030387 >10k+ 2.4411857172454537E-4 3.0431504788107997 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28502 3.0351498939369415 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9546 1.016544133307208 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9000 0.9584011313392911 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8749 0.9316723886763842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8390 0.8934428324374059 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6837 0.7280653927740814 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5989 0.6377627083990016 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5802 0.6178492626700629 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5743 0.6115664108090609 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5705 0.6075198282545172 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5577 0.5938892343865807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5442 0.5795132174164913 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5418 0.5769574810662532 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4646 0.4947479618002607 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3723 0.39645860133068667 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2902 0.3090311203496247 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2786 0.2966783946568072 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2711 0.2886917185623131 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2686 0.2860294931974817 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2527 0.2690977398771543 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2359 0.2512075854254875 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2337 0.24886482710443592 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2268 0.24151708509750133 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2252 0.23981326086400925 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2031 0.21627918863890003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1865 0.19860201221641974 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1677 0.1785820774728879 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1625 0.17304464871403866 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1340 0.1426952795549611 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1216 0.12949064174539754 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1210 0.12885170765783802 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1073 0.11426271265856214 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 983 0.10467870134516924 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 966 0.10286838809708392 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 944 0.10052562977603231 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.324450729662728E-5 0.0 20-21 0.0 0.0 0.0 1.0648901459325456E-4 0.0 22-23 0.0 0.0 0.0 1.0648901459325456E-4 0.0 24-25 0.0 0.0 0.0 4.2595605837301824E-4 0.0 26-27 0.0 0.0 0.0 8.519121167460365E-4 0.0 28-29 0.0 0.0 0.0 0.001544090711602191 0.0 30-31 0.0 0.0 0.0 0.003620626496170655 0.0 32-33 0.0 0.0 0.0 0.006921785948561547 0.0 34-35 0.0 0.0 0.0 0.011607302590664746 0.0 36-37 0.0 0.0 0.0 0.016559041769251083 0.0 38-39 0.0 0.0 0.0 0.026462520126423757 0.0 40-41 0.0 0.0 0.0 0.041637204705962536 0.0 42-43 0.0 0.0 0.0 0.06357394171217298 0.0 44-45 0.0 0.0 0.0 0.08492498913812052 0.0 46-47 0.0 0.0 0.0 0.12395321298654832 0.0 48-49 0.0 0.0 0.0 0.17032917884191068 0.0 50-51 0.0 0.0 0.0 0.1996669023623523 0.0 52-53 0.0 0.0 0.0 0.23901459325455987 0.0 54-55 0.0 0.0 0.0 0.2979030183246296 0.0 56-57 0.0 0.0 0.0 0.39102766158643076 0.0 58-59 0.0 0.0 0.0 0.4970374756140157 0.0 60-61 0.0 0.0 0.0 0.6275930075053457 0.0 62-63 0.0 0.0 0.0 0.7626743225168892 0.0 64-65 0.0 0.0 0.0 0.8870002470545139 0.0 66-67 0.0 0.0 0.0 1.0537087994002539 0.0 68-69 0.0 0.0 0.0 1.255132770503395 0.0 70-71 0.0 0.0 0.0 1.4247165262431527 0.0 72-73 0.0 0.0 0.0 1.6136280381315864 0.0 74-75 0.0 0.0 0.0 1.8104729816072174 0.0 76-77 0.0 5.324450729662728E-5 0.0 2.028722217016093 0.0 78-79 0.0 1.0648901459325456E-4 0.0 2.3059663665096313 0.0 80-81 0.0 1.0648901459325456E-4 0.0 2.6491804605436906 0.0 82-83 0.0 1.0648901459325456E-4 0.0 2.9852597906000016 0.0 84-85 0.0 1.0648901459325456E-4 0.0 3.386936353645758 0.0 86-87 0.0 1.0648901459325456E-4 0.0 3.8287060306858747 0.0 88-89 0.0 1.0648901459325456E-4 0.0 4.336871608324886 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 190 0.0 38.74775 94-95 GACCGAT 55 0.0025818055 34.538853 7 TAAGGTG 490 0.0 27.137669 5 GGTAAGG 450 0.0 26.386656 3 GTAAGGT 485 0.0 25.45905 4 GTACATG 7645 0.0 24.114208 1 GAGTACT 7720 0.0 23.842815 12-13 TACCGAA 50 0.0016533114 23.751785 28-29 TACATGG 7690 0.0 23.535578 2 AGGTAAG 465 0.0 23.49639 2 ACATGGG 7655 0.0 22.708725 3 GTACTTT 8085 0.0 22.532616 14-15 AGTACTT 7735 0.0 22.384136 12-13 ATGGGAG 1745 0.0 22.31665 5 AAGGTAA 490 0.0 21.332678 1 CATGGGA 5095 0.0 20.879229 4 ACTTTTT 8960 0.0 20.489033 16-17 TACTTTT 8455 0.0 20.366697 14-15 CTGGTCG 140 4.8535987E-4 20.35542 9 AAGGTGT 685 0.0 20.105644 6 >>END_MODULE