##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936382_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1074769 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.732509032173425 31.0 31.0 34.0 30.0 34.0 2 31.76162226487738 33.0 31.0 34.0 30.0 34.0 3 31.782035953772393 33.0 31.0 34.0 30.0 34.0 4 35.435608023677645 37.0 35.0 37.0 33.0 37.0 5 35.41480076183812 37.0 35.0 37.0 33.0 37.0 6 35.56549360839399 37.0 35.0 37.0 33.0 37.0 7 35.44888250405436 37.0 35.0 37.0 33.0 37.0 8 35.44268768451639 37.0 35.0 37.0 33.0 37.0 9 36.978312549022164 39.0 37.0 39.0 33.0 39.0 10-11 36.86344554039054 39.0 37.0 39.0 33.0 39.0 12-13 36.794834983145215 39.0 37.0 39.0 33.0 39.0 14-15 37.797157807863826 40.0 37.0 41.0 33.0 41.0 16-17 37.80004819640314 40.0 37.0 41.0 33.0 41.0 18-19 37.87042564495255 40.0 37.5 41.0 33.0 41.0 20-21 37.79402225036263 40.0 37.5 41.0 32.5 41.0 22-23 37.68597670755297 40.0 37.0 41.0 32.5 41.0 24-25 37.50186458671584 39.5 37.0 41.0 32.0 41.0 26-27 37.2864401559777 39.0 36.5 41.0 31.5 41.0 28-29 37.40052513609901 39.0 37.0 41.0 32.0 41.0 30-31 37.297823997528766 39.0 36.5 41.0 32.0 41.0 32-33 37.027685484043545 39.0 35.5 41.0 31.0 41.0 34-35 36.76653262235885 39.0 35.0 41.0 31.0 41.0 36-37 36.47563941646996 39.0 35.0 40.5 30.5 41.0 38-39 36.24833754974325 38.0 35.0 40.0 30.0 41.0 40-41 36.11162212531251 38.0 35.0 40.0 30.0 41.0 42-43 35.760606232595094 38.0 35.0 40.0 29.5 41.0 44-45 35.34908664094331 38.0 35.0 40.0 27.5 41.0 46-47 34.78423177445572 38.0 34.0 40.0 25.0 41.0 48-49 34.32391006811696 38.0 34.0 40.0 23.0 41.0 50-51 33.51041153959595 37.5 33.0 39.5 22.5 40.5 52-53 33.704402062210576 37.5 33.5 39.5 22.0 40.5 54-55 34.065750872978285 38.0 34.0 40.0 22.0 41.0 56-57 33.888408578959755 38.0 33.5 40.0 21.0 41.0 58-59 33.63612832152769 38.0 33.0 40.0 20.0 41.0 60-61 33.20012300317556 37.0 33.0 40.0 18.0 41.0 62-63 32.938233238956464 36.5 33.0 40.0 17.0 41.0 64-65 32.64016081595208 36.0 32.0 39.0 14.0 41.0 66-67 32.165719796533025 35.5 32.0 39.0 10.0 41.0 68-69 31.7560289699461 35.0 31.0 38.5 7.5 40.0 70-71 31.247339660894575 35.0 31.0 37.5 6.5 40.0 72-73 30.82781276720858 35.0 30.5 37.0 2.0 39.5 74-75 30.44963336307616 35.0 30.0 36.5 2.0 39.0 76-77 30.06504979209486 34.5 30.0 36.0 2.0 39.0 78-79 29.576970027978106 34.0 29.0 36.0 2.0 37.5 80-81 29.094978548878878 34.0 29.0 35.0 2.0 37.0 82-83 28.666810728630992 34.0 28.5 35.0 2.0 37.0 84-85 28.275212161869202 34.0 27.5 35.0 2.0 36.0 86-87 28.066611057818008 34.0 27.5 35.0 2.0 36.0 88-89 27.6179323184796 34.0 26.5 35.0 2.0 35.5 90-91 27.19492793335126 33.5 25.5 35.0 2.0 35.0 92-93 26.792385619607565 33.0 25.0 35.0 2.0 35.0 94-95 26.342121423301194 33.0 24.5 35.0 2.0 35.0 96-97 25.769283446024218 33.0 23.0 35.0 2.0 35.0 98-99 25.17168759054271 33.0 19.0 35.0 2.0 35.0 100-101 23.20662114370623 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34636251095473725 1101 2 0.24515531857850448 1101 3 0.28568010055465365 1101 4 0.31283510411678606 1101 5 0.2761650151912889 1101 6 0.2507718768010889 1101 7 0.24160641671826966 1101 8 0.2320924134930351 1101 9 0.4352076909355418 1101 10-11 0.4080292773636316 1101 12-13 0.497792178576411 1101 14-15 0.6748300846565556 1101 16-17 0.6909252023183328 1101 18-19 0.6269619149060688 1101 20-21 0.5488609121356589 1101 22-23 0.5260318642467823 1101 24-25 0.5742878489478613 1101 26-27 0.5262943533299591 1101 28-29 0.6356454506146392 1101 30-31 0.5198189790517844 1101 32-33 0.3210314638381604 1101 34-35 0.349471837198692 1101 36-37 0.6039478643361704 1101 38-39 0.4704369626821645 1101 40-41 0.4515114991785083 1101 42-43 0.24961916057187494 1101 44-45 0.28787702403384685 1101 46-47 0.5124265526117213 1101 48-49 0.5913304238657773 1101 50-51 0.9358380391866845 1101 52-53 0.8284843115768581 1101 54-55 0.7884352148078975 1101 56-57 0.7787856466479894 1101 58-59 0.7768028762900556 1101 60-61 0.7763096746218352 1101 62-63 0.7310430529734617 1101 64-65 0.8584270089445383 1101 66-67 0.6075798806219197 1101 68-69 0.7867404458041705 1101 70-71 0.7845566853155042 1101 72-73 0.7359230812521496 1101 74-75 0.5486134100288886 1101 76-77 0.6049054134964997 1101 78-79 0.5001829521771128 1101 80-81 0.510956432269623 1101 82-83 0.4972651942998887 1101 84-85 0.5351127976794032 1101 86-87 0.5671089441422161 1101 88-89 0.4503721546102888 1101 90-91 0.3384069746364098 1101 92-93 0.3229281218738258 1101 94-95 0.3009214298892502 1101 96-97 0.25399973458640446 1101 98-99 -0.10620140126543376 1101 100-101 0.12338948258375737 1102 1 0.2176801636287955 1102 2 0.20029889499469533 1102 3 0.1905173560309379 1102 4 0.19627122264706998 1102 5 0.038678436469581357 1102 6 0.09313347920740256 1102 7 -0.008741275457694542 1102 8 -0.02680208434451714 1102 9 0.3211354091126424 1102 10-11 0.3298520533311873 1102 12-13 0.409936798175103 1102 14-15 0.1838385017532218 1102 16-17 0.11339482773485798 1102 18-19 0.12263595025897445 1102 20-21 0.10841027086031119 1102 22-23 -0.1658692076327739 1102 24-25 0.04524087521562592 1102 26-27 0.13273075967857295 1102 28-29 0.053682504151815635 1102 30-31 -0.17204521477923862 1102 32-33 -0.041692543813816485 1102 34-35 -0.209548088392431 1102 36-37 -0.22235885844730063 1102 38-39 -0.14567569255004287 1102 40-41 -0.4349653628132373 1102 42-43 -0.17882089373635068 1102 44-45 -0.06957024353589247 1102 46-47 -0.015598856787612192 1102 48-49 0.11979519070538203 1102 50-51 0.11545278650635282 1102 52-53 -0.1948719977697735 1102 54-55 -0.05530952636119224 1102 56-57 0.13162400592241852 1102 58-59 0.1378822077546218 1102 60-61 -0.23741955657258274 1102 62-63 0.027643024064460064 1102 64-65 0.05504868297904153 1102 66-67 0.0014397703184059196 1102 68-69 0.07846806368993242 1102 70-71 0.20367867408507934 1102 72-73 0.09735169446372893 1102 74-75 -0.00846088058793626 1102 76-77 0.26140970512641104 1102 78-79 0.20592282968264897 1102 80-81 0.20048204916095713 1102 82-83 -0.004318866244545916 1102 84-85 0.06216772096246714 1102 86-87 -0.023716602765603056 1102 88-89 -0.2782933644567258 1102 90-91 -0.2728201869364959 1102 92-93 -0.1814679958425991 1102 94-95 0.04023834669788329 1102 96-97 -0.12395299962417283 1102 98-99 -0.32465975471567177 1102 100-101 -0.023232339810711267 1103 1 0.10740524485091996 1103 2 0.17190739155394752 1103 3 0.20322765951602761 1103 4 0.0791003108274353 1103 5 0.32700845838463266 1103 6 0.24398492585661558 1103 7 0.2338762514671231 1103 8 0.07111172213790695 1103 9 0.3329452342185988 1103 10-11 0.29105686952615173 1103 12-13 0.33867045781814653 1103 14-15 0.3082835904793413 1103 16-17 0.5562421642460222 1103 18-19 0.38308589099452917 1103 20-21 0.1405128367120554 1103 22-23 0.06128690939531367 1103 24-25 0.25951145361949557 1103 26-27 0.2469218214073905 1103 28-29 0.25958485852758173 1103 30-31 0.0977439914183762 1103 32-33 0.22770334263721992 1103 34-35 0.1340251309030478 1103 36-37 0.1173672886819972 1103 38-39 0.16584015535779173 1103 40-41 0.12085619579886497 1103 42-43 0.25273784627609786 1103 44-45 0.4576065520913275 1103 46-47 0.30117170029812 1103 48-49 0.33681410416223656 1103 50-51 -0.06928235687947648 1103 52-53 0.2735730994835919 1103 54-55 0.42786555528729764 1103 56-57 0.44261819886014564 1103 58-59 0.5040085305454198 1103 60-61 0.632024295701207 1103 62-63 0.3601013897303105 1103 64-65 0.42212813996329146 1103 66-67 0.4119039942100713 1103 68-69 0.1972851625464287 1103 70-71 0.4297341353670667 1103 72-73 0.28427727708858797 1103 74-75 0.1478437471546421 1103 76-77 0.12133159544882588 1103 78-79 0.4534940535999006 1103 80-81 0.5946434360340938 1103 82-83 0.5827868546924329 1103 84-85 0.3545643919211834 1103 86-87 0.44430810069902194 1103 88-89 0.4268127572613487 1103 90-91 0.7694611528661817 1103 92-93 0.789430180177046 1103 94-95 0.8228823933931437 1103 96-97 0.8013499311941885 1103 98-99 0.867115065998739 1103 100-101 0.9599467922850344 1104 1 0.12625523544004125 1104 2 0.1272057164174889 1104 3 0.14921235115289377 1104 4 0.1390632947034689 1104 5 0.12218067746989192 1104 6 0.055296802385882415 1104 7 0.22960998633129037 1104 8 0.13447517028396305 1104 9 0.34476843517223443 1104 10-11 0.3291050532265061 1104 12-13 0.2128642591858565 1104 14-15 0.09790652792835886 1104 16-17 0.10867017102184917 1104 18-19 0.1915729353511395 1104 20-21 0.12499743424012877 1104 22-23 0.055958783415015034 1104 24-25 0.1654952041684652 1104 26-27 0.09608425317852465 1104 28-29 -0.0030963008927145097 1104 30-31 0.022077387490902822 1104 32-33 -0.05766117207512167 1104 34-35 -0.09231121391155739 1104 36-37 0.10656203113760654 1104 38-39 0.00820950098800921 1104 40-41 0.03686410093720838 1104 42-43 0.08312714296974377 1104 44-45 0.006828586095970479 1104 46-47 0.05833965311824585 1104 48-49 -0.01648440891887759 1104 50-51 -0.03686658424631872 1104 52-53 0.08327101026846151 1104 54-55 0.2902581144189824 1104 56-57 0.3439043523202088 1104 58-59 0.4463198424219499 1104 60-61 0.30158010221354203 1104 62-63 0.23123351847113582 1104 64-65 0.21656474200859321 1104 66-67 0.12026399170050439 1104 68-69 0.29436151614451944 1104 70-71 0.26157741286251834 1104 72-73 0.09000222846571759 1104 74-75 -0.058129024035523 1104 76-77 0.05921352028727256 1104 78-79 0.17999352031675997 1104 80-81 0.4234093561043615 1104 82-83 0.3483759757163334 1104 84-85 0.4537958368048045 1104 86-87 0.2725893605020211 1104 88-89 0.1776440515709048 1104 90-91 0.3121316466236337 1104 92-93 0.15900794540698726 1104 94-95 0.16392951052721827 1104 96-97 0.4258370382899912 1104 98-99 0.24881247487006064 1104 100-101 0.4508433353554899 1105 1 0.17966256137715675 1105 2 0.16598642053378398 1105 3 0.05458933971679869 1105 4 0.13674747629973183 1105 5 0.21201232299907957 1105 6 0.18742319221919956 1105 7 0.16771977752225808 1105 8 -0.04247120485428013 1105 9 0.3254006480186078 1105 10-11 0.2632540698276884 1105 12-13 0.1765887665483561 1105 14-15 0.28103948998028017 1105 16-17 0.26918194305647347 1105 18-19 0.2556674057325523 1105 20-21 -0.21838007052765107 1105 22-23 -0.30423667429506196 1105 24-25 -0.32419871938164135 1105 26-27 -0.23131324257344232 1105 28-29 -0.12389105204237438 1105 30-31 -0.1290625965564729 1105 32-33 -0.27998557021333426 1105 34-35 -0.2872438462623279 1105 36-37 -0.401179759420522 1105 38-39 -0.3148408278764592 1105 40-41 -0.649053725286663 1105 42-43 -0.3205970297222578 1105 44-45 -0.16178474727792747 1105 46-47 -0.15815919657870126 1105 48-49 -0.16424675635857255 1105 50-51 -0.05856616271361759 1105 52-53 0.09357581902708745 1105 54-55 0.06983550671720451 1105 56-57 0.042056159870476506 1105 58-59 -0.028807294335059908 1105 60-61 -0.010244566957076984 1105 62-63 -0.311044492308298 1105 64-65 -0.09767132948717006 1105 66-67 -0.12113941352106394 1105 68-69 -0.10370723227403289 1105 70-71 -0.019527293872318552 1105 72-73 -0.1962340925116841 1105 74-75 -0.1789833566746637 1105 76-77 0.002914083497149278 1105 78-79 -0.0963449207982201 1105 80-81 0.06786404349423236 1105 82-83 0.02316372616775908 1105 84-85 0.022550593816276887 1105 86-87 0.0988013113825339 1105 88-89 0.19223543157454515 1105 90-91 0.32328783683614404 1105 92-93 -0.006431342576465937 1105 94-95 0.25664337891753775 1105 96-97 0.3347678725474701 1105 98-99 0.2583249765877831 1105 100-101 0.21638256811830914 1106 1 -0.040767501157816355 1106 2 -0.03553474869001505 1106 3 -0.11325756368901452 1106 4 0.07434974628188229 1106 5 0.18018470506885365 1106 6 0.17848285010396836 1106 7 0.3937338577939755 1106 8 0.29147135491211884 1106 9 0.3584059921394882 1106 10-11 0.2934674050802002 1106 12-13 0.27375536152491975 1106 14-15 0.21530663577117792 1106 16-17 0.11659331651060967 1106 18-19 0.14741869183075806 1106 20-21 0.11094642741899463 1106 22-23 0.16826779866548947 1106 24-25 0.19418909564338094 1106 26-27 0.037707649095274576 1106 28-29 0.040865366174834605 1106 30-31 0.05259957622840261 1106 32-33 -0.030801080047154983 1106 34-35 -0.1179034454560508 1106 36-37 -0.0514143283342392 1106 38-39 -0.030900025494005945 1106 40-41 -0.15205104123137403 1106 42-43 -0.05408082859496943 1106 44-45 -0.33142055595627795 1106 46-47 -0.18728100113863633 1106 48-49 -0.3569603635871701 1106 50-51 -0.09461122315367732 1106 52-53 -0.2033369873923263 1106 54-55 -0.11862021027591396 1106 56-57 -0.050152659742153105 1106 58-59 -0.04302871466949654 1106 60-61 0.05246084472545931 1106 62-63 0.07230370905107719 1106 64-65 -0.15872982136163927 1106 66-67 -0.06639132474583676 1106 68-69 -0.04516570473359849 1106 70-71 -0.047933034213539116 1106 72-73 0.0895455471632367 1106 74-75 0.01744112016886845 1106 76-77 0.03629377829255631 1106 78-79 0.2110148357789967 1106 80-81 0.22952495542586604 1106 82-83 0.18727437552391635 1106 84-85 0.4128792357713351 1106 86-87 0.46048738458901184 1106 88-89 0.5922517222447361 1106 90-91 0.7143908435248036 1106 92-93 0.46140406809626455 1106 94-95 0.5623922002150259 1106 96-97 0.6098403018713441 1106 98-99 0.5469671994385727 1106 100-101 0.8803029736890515 1107 1 0.21350287230216836 1107 2 0.4139932175852117 1107 3 0.3397609583263481 1107 4 0.37321254696485795 1107 5 0.3988356343566153 1107 6 0.38994232627361924 1107 7 0.5127904118083535 1107 8 0.5028726684319054 1107 9 0.6089778584673837 1107 10-11 0.69368220851036 1107 12-13 0.6438478879431173 1107 14-15 0.6463779200741939 1107 16-17 0.7071920013973241 1107 18-19 0.8045100334837372 1107 20-21 0.8730771244704627 1107 22-23 0.7465041318370922 1107 24-25 0.8734173011822151 1107 26-27 0.8163975712609002 1107 28-29 0.7406409220862642 1107 30-31 0.5714912829516052 1107 32-33 0.608686499040104 1107 34-35 0.5137361596434573 1107 36-37 0.7675113995553815 1107 38-39 0.8250908469537066 1107 40-41 0.7926956365260338 1107 42-43 0.8638146236435347 1107 44-45 0.9063391940558674 1107 46-47 0.9546378563875706 1107 48-49 0.893562104325774 1107 50-51 0.8363038984182154 1107 52-53 0.5552294546709007 1107 54-55 0.5839049251514368 1107 56-57 0.5951631355603766 1107 58-59 0.811131627804933 1107 60-61 1.0160330990699293 1107 62-63 1.0448361984682677 1107 64-65 0.7354173428904787 1107 66-67 0.8607388424677964 1107 68-69 1.0916679046731375 1107 70-71 1.0632870986681944 1107 72-73 1.1137842339302786 1107 74-75 0.7511402407974117 1107 76-77 0.979640967084535 1107 78-79 0.990687945751823 1107 80-81 0.9311242689472472 1107 82-83 1.2506405906731324 1107 84-85 1.2097019615918079 1107 86-87 0.8514710584128693 1107 88-89 1.1332149594662795 1107 90-91 1.174802794435525 1107 92-93 0.8834095481333293 1107 94-95 1.1052995040893734 1107 96-97 0.8934895858731906 1107 98-99 0.9297419721461075 1107 100-101 1.1748948033505648 1108 1 0.1793635497039645 1108 2 -0.004100810770044205 1108 3 -0.014834541440908566 1108 4 0.043044261613495394 1108 5 0.19867626426296425 1108 6 0.1369275095886735 1108 7 0.2778783020481228 1108 8 -0.019523957971458117 1108 9 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2316 52-53 -1.3902591069251784 2316 54-55 -1.1885977749909458 2316 56-57 -0.8473276741749487 2316 58-59 -1.0547443011767896 2316 60-61 -1.4150186081326375 2316 62-63 -1.175545021115834 2316 64-65 -0.9660153743120468 2316 66-67 -1.367417948826919 2316 68-69 -1.4036555744795294 2316 70-71 -1.1177998885318026 2316 72-73 -1.0102763051480785 2316 74-75 -1.1219523688465358 2316 76-77 -1.5365410246075513 2316 78-79 -1.952609064320793 2316 80-81 -1.8802800426177448 2316 82-83 -1.7144112534577403 2316 84-85 -1.8582955178095624 2316 86-87 -1.847732382120423 2316 88-89 -2.1825046896060307 2316 90-91 -2.3211431496222765 2316 92-93 -2.374185431907275 2316 94-95 -2.618479773068888 2316 96-97 -2.7264554192476496 2316 98-99 -3.184160722127917 2316 100-101 -4.182275698938792 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1784.0 3 662.0 4 666.0 5 702.0 6 752.0 7 940.0 8 1190.0 9 1559.0 10 2062.0 11 2960.0 12 4190.0 13 5651.0 14 6754.0 15 9042.0 16 12239.0 17 16792.0 18 14217.0 19 7943.0 20 7396.0 21 7627.0 22 8275.0 23 9111.0 24 10181.0 25 11647.0 26 13442.0 27 16389.0 28 19276.0 29 23177.0 30 28854.0 31 35923.0 32 45967.0 33 60030.0 34 81699.0 35 113403.0 36 151707.0 37 182025.0 38 135756.0 39 22768.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.919574628060104 18.078121335968646 14.551159648476322 19.451144387494928 2 15.36863966770509 22.05278084837928 37.94305057745918 24.635528906456454 3 22.537355375284505 26.032914044951138 30.378308665479338 21.051421914285022 4 11.791457936583287 16.067609000385346 39.35453304962796 32.78640001340341 5 11.835436026946997 39.94621398790048 33.376277743593946 14.842072241558585 6 28.690331662483338 36.503820859060205 17.192096854680738 17.613750623775722 7 26.19135371585462 35.60900926055191 20.469263344036484 17.730373679556983 8 26.495336714265903 29.50895196364303 19.913314353203525 24.08239696888754 9 26.154994351355022 18.097904883146565 22.44289471186327 33.30420605363514 10-11 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31.000917427778152 21.212539916482438 36-37 21.916394656820966 24.139633168204437 33.117260746094786 20.826711428879815 38-39 21.39227238024255 25.753428736391015 32.08550079161368 20.76879809175275 40-41 20.859203955565025 26.73635180262371 31.613024185406875 20.79142005640439 42-43 20.264681978280763 27.087404973103336 29.388752760496168 23.259160288119737 44-45 21.19429468114257 25.362327942784912 29.943200823632253 23.500176552440262 46-47 23.083407653668633 26.8399923888533 29.606988484186168 20.4696114732919 48-49 22.420002167918803 26.467264658690027 29.17018488253462 21.942548290856546 50-51 19.98434049331633 27.417163024024738 31.295007706468454 21.30348877619048 52-53 21.779384131984877 28.52470715541477 27.791101171936607 21.904807540663747 54-55 20.21399534586408 26.571561759415975 29.411403470411067 23.80303942430888 56-57 22.508144943947247 26.151934432910455 28.132075796478517 23.207844826663777 58-59 24.156057437548416 25.98037185997446 27.795846873524344 22.067723828952786 60-61 21.98518178045662 26.552768158041157 30.878253523118694 20.583796538383524 62-63 21.203301761929065 27.10197025474071 30.026531517051026 21.6681964662792 64-65 20.464822858163707 29.224169374318436 28.845237696467585 21.465770071050272 66-67 21.499780646764062 27.023946488045365 27.61948005606883 23.856792809121746 68-69 20.74435790442365 27.097690868665687 28.913387453283974 23.244563773626687 70-71 22.91717263076206 26.989916077422045 27.659649700701884 22.433261591114015 72-73 22.01924025286445 25.903486097851918 30.212165705491444 21.865107943792186 74-75 20.437342578351437 29.771963663803653 28.20521409344205 21.585479664402865 76-77 21.20823303803793 27.081823282403327 27.99143609216343 23.71850758739531 78-79 21.48613637770372 27.345069442602295 28.7947474651577 22.37404671453629 80-81 22.806948154076668 27.2494485961304 27.752924534447622 22.190678715345314 82-83 21.711796837235962 26.550432227049814 29.679838879638453 22.057932056075767 84-85 21.266095928172742 27.863164197658953 28.59700406489502 22.273735809273283 86-87 21.28318540839983 28.448344445731628 27.95895228484587 22.309517861022677 88-89 20.869687797461474 28.159407249708995 26.55284776764903 24.418057185180498 90-91 21.259963048726643 26.624923173268535 27.55284585754451 24.562267920460314 92-93 22.704738366020035 27.96440421115714 27.89703165253999 21.433825770282837 94-95 21.725897346308614 27.220539857012653 27.96771781796121 23.085844978717525 96-97 19.41663243311897 27.549453325523586 30.048417014391116 22.985497226966327 98-99 21.11048188168243 28.77088226083323 26.63599522199261 23.482640635491737 100-101 20.467919273290196 27.07647497152708 27.92277335279467 24.532832402388046 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 592.0 1 473.0 2 578.5 3 788.5 4 688.0 5 672.5 6 1162.5 7 2334.5 8 3772.0 9 4366.5 10 3600.0 11 2756.5 12 2679.0 13 2788.0 14 2881.5 15 2916.0 16 2819.0 17 2869.5 18 2999.5 19 3074.5 20 3295.0 21 3608.0 22 4034.0 23 4647.0 24 5340.0 25 6210.0 26 7353.0 27 8629.0 28 9630.0 29 10887.5 30 12604.0 31 14489.0 32 17197.0 33 18594.0 34 19091.0 35 19967.0 36 21283.0 37 22759.0 38 23224.0 39 23259.5 40 23570.5 41 24871.5 42 25610.0 43 26182.5 44 27490.5 45 29692.0 46 32903.5 47 51657.5 48 92684.5 49 88666.5 50 47924.5 51 31426.5 52 26721.5 53 25969.0 54 25690.5 55 24222.0 56 22544.0 57 21063.0 58 20441.0 59 18929.0 60 16516.0 61 14816.0 62 13033.0 63 9968.5 64 7110.0 65 5732.0 66 4336.0 67 3271.0 68 2470.0 69 1695.0 70 1320.0 71 1096.5 72 918.0 73 907.5 74 745.0 75 576.5 76 448.5 77 280.0 78 193.0 79 129.5 80 90.5 81 78.0 82 51.0 83 30.0 84 20.0 85 13.5 86 10.0 87 8.0 88 5.0 89 6.0 90 5.5 91 4.0 92 2.5 93 0.5 94 0.5 95 1.0 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012374752156044694 2 0.005675638206907717 3 0.00846693568571479 4 0.038612948456831185 5 0.047638143638307394 6 0.06187376078022347 7 0.02968079652464855 8 0.02902949377959357 9 0.0162825686263746 10-11 0.047963795010834885 12-13 0.0440094569158582 14-15 0.01925995260376881 16-17 0.021679077085401607 18-19 0.028750364031712862 20-21 0.023028204200158362 22-23 0.014840398262324275 24-25 0.003582165097802411 26-27 0.0031634704759813505 28-29 0.0017678217365778134 30-31 0.006280419327315916 32-33 0.0012095622408163987 34-35 0.0023260812323392284 36-37 0.004931292212559164 38-39 0.0060943328287287775 40-41 0.0027912974788070737 42-43 6.978243697017684E-4 44-45 0.0016282568626374597 46-47 0.002744775854160289 48-49 5.117378711146301E-4 50-51 0.0020934731091053053 52-53 8.839108682889067E-4 54-55 0.0024191244816327974 56-57 0.0020934731091053053 58-59 0.003396078599215273 60-61 0.0010234757422292603 62-63 8.839108682889067E-4 64-65 0.0034426002238620575 66-67 0.0017213001119310287 68-69 0.005349986834380225 70-71 0.008187805937834082 72-73 0.004000859719623473 74-75 0.0026052109802199358 76-77 0.0024191244816327974 78-79 0.036519475347725885 80-81 0.022237336581163024 82-83 0.004140424593563826 84-85 0.007396938318838746 86-87 0.006373462576609485 88-89 0.004652162464678457 90-91 0.010281279046939389 92-93 0.013723879270801447 94-95 0.005443030083673794 96-97 0.004047381344270257 98-99 0.024423852939561896 100-101 0.04675423277001849 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1074769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.59969193629946 #Duplication Level Percentage of deduplicated Percentage of total 1 75.55258673971788 33.69622093581961 2 13.254358314181664 11.822805952516633 3 4.6805883600686995 6.262583968190792 4 2.1500845340665844 3.835724314254865 5 1.162167226224404 2.591615013403603 6 0.7033525008092517 1.8821582915231065 7 0.4685536299822368 1.4628143286989825 8 0.31087243877034965 1.1091852000514968 9 0.24212771550185716 0.9718939368560507 >10 1.2909353398650738 10.60044216737147 >50 0.11071021886654973 3.4172944705648063 >100 0.061316061758540194 5.3572611345722905 >500 0.005231745841914758 1.6481798283494202 >1k 0.004603936340884987 4.903099321744158 >5k 0.0016741586694127225 4.929792760268873 >10k+ 8.370793347063613E-4 5.508928375813816 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21544 2.004523762780653 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15704 1.4611511869062095 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11599 1.0792086485561083 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10177 0.946901148060653 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9588 0.8920986742267408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8418 0.7832380725532649 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7139 0.66423575670679 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6093 0.5669125179457166 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5718 0.5320212994606283 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5615 0.5224378447833906 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5206 0.4843831558223209 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5042 0.46912406293817555 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4487 0.41748505958024473 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4448 0.4138563728577955 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3370 0.31355575011932796 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3325 0.3093688039011173 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3318 0.3087175011560624 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3067 0.2853636455833765 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2772 0.25791588704177365 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2564 0.23856289118871127 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2540 0.2363298532056656 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2433 0.22637422553125366 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2370 0.22051250082575882 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2159 0.20088037522481575 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2038 0.18962214206029387 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1891 0.17594478441413924 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1772 0.16487263774820451 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1722 0.16022047528352604 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1717 0.1597552590370582 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1575 0.14654311763737138 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.13900661444459228 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1294 0.12039796458587847 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1091 0.10151018497928392 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1086 0.10104496873281608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.304324929356913E-5 0.0 24-25 0.0 0.0 0.0 1.8608649858713826E-4 0.0 26-27 0.0 0.0 0.0 5.117378711146303E-4 0.0 28-29 0.0 0.0 0.0 0.0013026054901099679 0.0 30-31 0.0 0.0 0.0 0.0026052109802199358 0.0 32-33 0.0 0.0 0.0 0.005629116582260932 0.0 34-35 0.0 0.0 0.0 0.009723019551177974 0.0 36-37 0.0 0.0 0.0 0.014096052267975723 0.0 38-39 0.0 0.0 0.0 0.023819071819153697 0.0 40-41 0.0 0.0 0.0 0.03512382660832235 0.0 42-43 0.0 0.0 0.0 0.052755522349453694 0.0 44-45 0.0 0.0 0.0 0.0681076584828926 0.0 46-47 0.0 0.0 0.0 0.09862584425118329 0.0 48-49 0.0 0.0 0.0 0.1341218438566799 0.0 50-51 0.0 0.0 0.0 0.1564522236871365 0.0 52-53 0.0 0.0 0.0 0.18738910407724824 0.0 54-55 0.0 0.0 0.0 0.23637637483031237 0.0 56-57 0.0 0.0 0.0 0.3144396609876169 0.0 58-59 0.0 0.0 0.0 0.4053894371720807 0.0 60-61 0.0 0.0 0.0 0.5068531005267178 0.0 62-63 0.0 0.0 0.0 0.6144576183347306 0.0 64-65 0.0 0.0 0.0 0.7265282121088346 0.0 66-67 0.0 0.0 0.0 0.8656743914273672 0.0 68-69 0.0 0.0 0.0 1.0420378704633273 0.0 70-71 0.0 0.0 0.0 1.1834636093895525 0.0 72-73 0.0 0.0 0.0 1.3420558278104413 0.0 74-75 0.0 0.0 0.0 1.514185839003544 0.0 76-77 0.0 0.0 0.0 1.6973879968625816 0.0 78-79 0.0 0.0 0.0 1.928135255110633 0.0 80-81 0.0 0.0 0.0 2.226106260973288 0.0 82-83 0.0 0.0 0.0 2.5168664150156914 0.0 84-85 0.0 0.0 0.0 2.8738733625551163 0.0 86-87 0.0 0.0 0.0 3.2752154183829267 0.0 88-89 0.0 0.0 0.0 3.7223347528631736 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 185 2.8740033E-10 28.26328 5 GTACATG 9045 0.0 27.693857 1 TACATGG 9125 0.0 26.928915 2 ACATGGG 9035 0.0 26.145044 3 GTTCGAC 55 9.4542236E-5 25.919624 16-17 GAGTACT 7505 0.0 24.283152 12-13 CATGGGA 6110 0.0 24.0369 4 GCTTATG 265 3.092282E-11 23.317333 1 AGTACTT 7600 0.0 23.2918 12-13 GTACTTT 8005 0.0 22.706509 14-15 TAAGGTG 360 0.0 22.446468 5 ATGGGAT 2160 0.0 22.006342 5 GTACACG 130 2.9281853E-4 21.937609 1 CATGGGG 2535 0.0 21.749096 4 TCGACCG 55 0.0028737185 21.59667 18-19 ATGGGAG 2165 0.0 21.51641 5 TAGCGCG 155 3.8876013E-5 21.464832 4 GTAAGCG 135 9.968062E-10 21.120676 94-95 TACTTTT 8465 0.0 20.68668 14-15 GGCGACA 300 1.6552804E-10 20.596018 6 >>END_MODULE