##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936382_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1074769 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.356680365734405 31.0 31.0 34.0 30.0 34.0 2 31.552113989145575 31.0 31.0 34.0 30.0 34.0 3 31.795399755668427 31.0 31.0 34.0 30.0 34.0 4 35.61444273141484 37.0 35.0 37.0 33.0 37.0 5 35.458642740905255 37.0 35.0 37.0 33.0 37.0 6 35.62094273281049 37.0 35.0 37.0 33.0 37.0 7 35.561616496195924 37.0 35.0 37.0 33.0 37.0 8 35.55448566157007 37.0 35.0 37.0 33.0 37.0 9 37.11883949016021 39.0 37.0 39.0 33.0 39.0 10-11 36.989778268632605 39.0 37.0 39.0 33.0 39.0 12-13 36.8755821018284 39.0 37.0 39.0 33.0 39.0 14-15 37.76519605608275 39.0 37.0 41.0 32.0 41.0 16-17 37.75673609864073 39.0 37.0 41.0 32.5 41.0 18-19 37.94509843510559 39.5 37.0 41.0 33.0 41.0 20-21 38.05368083746367 40.0 37.0 41.0 33.0 41.0 22-23 37.95954758650464 40.0 37.0 41.0 33.0 41.0 24-25 37.78910770593495 39.5 37.0 41.0 32.5 41.0 26-27 37.49070404896308 39.0 36.5 41.0 32.0 41.0 28-29 37.40429013118168 39.0 36.0 41.0 32.0 41.0 30-31 37.36022903526246 39.0 36.0 41.0 32.0 41.0 32-33 37.073636288355914 39.0 35.0 41.0 31.5 41.0 34-35 36.78720776278438 39.0 35.0 40.0 31.0 41.0 36-37 36.46869327269395 38.5 35.0 40.0 30.5 41.0 38-39 36.242429303413104 38.0 35.0 40.0 30.0 41.0 40-41 36.02747288021891 38.0 35.0 40.0 30.0 41.0 42-43 35.67571403715589 38.0 35.0 40.0 29.0 41.0 44-45 35.24182731359018 38.0 34.5 40.0 27.0 41.0 46-47 34.6320576793711 38.0 34.0 40.0 24.5 41.0 48-49 33.941417644163536 38.0 33.0 40.0 22.0 41.0 50-51 33.66176359757306 38.0 33.0 40.0 20.5 41.0 52-53 33.42819759408766 37.5 33.0 40.0 19.5 41.0 54-55 33.17065388004306 37.0 33.0 40.0 18.5 41.0 56-57 32.803081871546354 37.0 32.0 40.0 15.5 41.0 58-59 32.40164025944179 36.0 31.5 39.0 12.0 40.5 60-61 31.870960643635982 36.0 31.0 39.0 8.0 40.0 62-63 31.6463221399203 35.0 31.0 39.0 8.0 40.0 64-65 31.21564308237398 35.0 30.0 38.0 7.0 40.0 66-67 30.729612130606668 35.0 29.5 38.0 3.5 40.0 68-69 30.22074696981398 34.0 29.0 37.0 2.0 39.0 70-71 29.62593869008131 34.0 28.5 36.5 2.0 39.0 72-73 29.163999892069832 34.0 28.0 36.0 2.0 39.0 74-75 28.823877502979713 34.0 27.5 35.5 2.0 38.0 76-77 27.50400783796332 31.5 26.0 34.5 2.0 36.0 78-79 28.307640990761733 33.0 27.5 35.0 2.0 37.0 80-81 28.074587655579943 33.0 27.0 35.0 2.0 36.5 82-83 27.683489661499358 33.0 26.5 35.0 2.0 36.0 84-85 27.224036979109002 33.0 26.0 35.0 2.0 35.5 86-87 26.909270736316362 33.0 25.0 35.0 2.0 35.0 88-89 26.44192659073717 32.0 25.0 35.0 2.0 35.0 90-91 25.858809660494487 32.0 23.5 34.0 2.0 35.0 92-93 25.36336366233116 32.0 21.0 34.0 2.0 35.0 94-95 24.730503019718654 31.0 18.5 34.0 2.0 35.0 96-97 23.93792247450382 31.0 6.0 34.0 2.0 35.0 98-99 23.061716517688915 31.0 2.0 34.0 2.0 35.0 100-101 20.303711774344066 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4004947849580027 1101 2 0.33183620078803244 1101 3 0.2510421654091317 1101 4 0.08421507891607405 1101 5 0.09409805259409865 1101 6 0.17612362997479636 1101 7 0.14955706885289288 1101 8 0.1304421542522789 1101 9 0.19717117844933796 1101 10-11 0.19844584744765115 1101 12-13 0.11041185520258523 1101 14-15 0.3202796471295528 1101 16-17 0.13872170546418516 1101 18-19 0.3156446529814332 1101 20-21 0.1267009510130137 1101 22-23 0.02885349879723975 1101 24-25 0.021896256676065207 1101 26-27 0.12795833165910864 1101 28-29 0.12937550327137615 1101 30-31 0.05540550328628768 1101 32-33 0.11799402915876556 1101 34-35 0.16317597662399663 1101 36-37 0.3154112376210918 1101 38-39 0.32894663739703134 1101 40-41 0.4183960201291157 1101 42-43 0.38114350131553465 1101 44-45 0.4097234825430931 1101 46-47 0.5201002715939396 1101 48-49 0.7500551305689598 1101 50-51 0.45320434933375253 1101 52-53 0.5996514177629422 1101 54-55 0.4194189830649293 1101 56-57 0.4958283639891192 1101 58-59 0.47835932514269786 1101 60-61 0.47037808390288305 1101 62-63 0.305195831504502 1101 64-65 0.4018139567664285 1101 66-67 0.4054056235576873 1101 68-69 0.27040608815948985 1101 70-71 0.3950817634597499 1101 72-73 0.37683201570226643 1101 74-75 0.27898984048016473 1101 76-77 0.6988392259187606 1101 78-79 0.6921560835910725 1101 80-81 0.5371143294358589 1101 82-83 0.6326056814301353 1101 84-85 0.6325511707079698 1101 86-87 0.6221236890053383 1101 88-89 0.5508523980841566 1101 90-91 0.6462339326300963 1101 92-93 0.6957228155523616 1101 94-95 0.45973080298658076 1101 96-97 0.4327311908640077 1101 98-99 0.4876274644628573 1101 100-101 0.6341826037562086 1102 1 0.40915858304941466 1102 2 0.4767118263902148 1102 3 0.09762482077274726 1102 4 0.1922584356323469 1102 5 0.19316181643554842 1102 6 0.18248530528943263 1102 7 0.25067540627178175 1102 8 0.09670425035816521 1102 9 0.11814745484310407 1102 10-11 0.16837752132531847 1102 12-13 0.13245136388525225 1102 14-15 0.2881535682876759 1102 16-17 0.4192928658976882 1102 18-19 0.3223583652995927 1102 20-21 0.17716834848673813 1102 22-23 0.16749122368445057 1102 24-25 0.12201146878631164 1102 26-27 -0.03369807768214628 1102 28-29 0.18247268710251774 1102 30-31 0.23189070310808546 1102 32-33 0.28759002801766087 1102 34-35 0.45203325306565745 1102 36-37 0.4967921043569632 1102 38-39 0.2639552232001705 1102 40-41 0.4501232226069831 1102 42-43 0.13356802106372356 1102 44-45 0.21571421555577075 1102 46-47 0.2145109582589697 1102 48-49 0.15739818586016696 1102 50-51 0.2336082868920144 1102 52-53 0.04566322636841136 1102 54-55 0.14503316153010104 1102 56-57 0.09205527335007702 1102 58-59 0.24649135025198632 1102 60-61 0.4153265754423465 1102 62-63 0.2436529387310351 1102 64-65 0.03924148822192208 1102 66-67 -0.006671598228166431 1102 68-69 -0.04611608296567837 1102 70-71 0.05622336372120529 1102 72-73 0.054658601553725816 1102 74-75 -0.32784289891877094 1102 76-77 -0.020386996323615847 1102 78-79 0.04990579354733882 1102 80-81 0.13932883505594873 1102 82-83 0.21141534280063468 1102 84-85 0.29408041436462895 1102 86-87 0.10128822741297938 1102 88-89 0.0041349047063086175 1102 90-91 -0.03955258924854732 1102 92-93 0.21379517876129484 1102 94-95 -0.02232611891715308 1102 96-97 0.4448882303112285 1102 98-99 0.3438577564310279 1102 100-101 0.23315862327529402 1103 1 0.3662728738800567 1103 2 0.46050278920284526 1103 3 0.18401190709417392 1103 4 0.1197532979597824 1103 5 0.06062819772333938 1103 6 0.11691282786534885 1103 7 0.1014309212754867 1103 8 0.045865594595021264 1103 9 0.025937957183842286 1103 10-11 0.13293269694418086 1103 12-13 0.20661803765796094 1103 14-15 0.12440421849105121 1103 16-17 0.15984530426693055 1103 18-19 0.3115119070959409 1103 20-21 0.2520195478454923 1103 22-23 0.1686963383334259 1103 24-25 0.017070562679933232 1103 26-27 0.235605074844905 1103 28-29 0.20801693930468446 1103 30-31 0.06777020443407622 1103 32-33 -0.0060531147883153835 1103 34-35 0.3090143054997654 1103 36-37 0.3707980393281005 1103 38-39 0.3508038473271924 1103 40-41 0.41420982543291274 1103 42-43 0.4942174996219393 1103 44-45 0.5222912891715836 1103 46-47 0.35075180211635626 1103 48-49 0.29117529187956137 1103 50-51 0.029727745240627712 1103 52-53 0.14590476093365368 1103 54-55 0.3835047359176329 1103 56-57 0.21070054797040427 1103 58-59 0.313668657514647 1103 60-61 0.185935251073996 1103 62-63 0.14742688966519424 1103 64-65 0.1856481019627445 1103 66-67 0.12444052667909844 1103 68-69 0.09341884041691628 1103 70-71 -0.028438018888309102 1103 72-73 0.026131941083534116 1103 74-75 0.0465883330140997 1103 76-77 0.09683038904872632 1103 78-79 0.1933967248091193 1103 80-81 0.2503524470316272 1103 82-83 0.2795148729487522 1103 84-85 0.42870704840032303 1103 86-87 0.29369982107211 1103 88-89 0.46594089254643833 1103 90-91 0.3716441260519119 1103 92-93 0.39841307235817425 1103 94-95 0.4221420360401673 1103 96-97 0.40062920634111165 1103 98-99 0.3685552519755362 1103 100-101 0.9409924014472182 1104 1 0.16514670536258436 1104 2 0.32314473662740184 1104 3 0.0981658690742222 1104 4 0.07086378167895901 1104 5 -0.12362740174965126 1104 6 0.05792230148351507 1104 7 0.051142320986890866 1104 8 -0.14429062599598552 1104 9 -0.16544795159337156 1104 10-11 -0.09532189870171237 1104 12-13 -0.09480614202708892 1104 14-15 0.07483711892395206 1104 16-17 -0.04649919664278457 1104 18-19 0.003479721889839027 1104 20-21 -0.2331975297628901 1104 22-23 -0.11582119727106033 1104 24-25 -0.30006194249604334 1104 26-27 -0.03900751063724783 1104 28-29 -0.12898036291001347 1104 30-31 -0.11761313203621881 1104 32-33 -0.27348363113187446 1104 34-35 -0.25549570140155 1104 36-37 -0.11495702686175235 1104 38-39 -0.2388771238755183 1104 40-41 -0.21795655890738175 1104 42-43 0.08054506094950398 1104 44-45 0.08807960713381391 1104 46-47 -0.014572245816381724 1104 48-49 0.07660254578507875 1104 50-51 -0.0010473974910425454 1104 52-53 -0.2637398215370226 1104 54-55 -0.30067871348320807 1104 56-57 -0.1262622476880395 1104 58-59 -0.03481932792985987 1104 60-61 -0.09707811237414887 1104 62-63 -0.4444719120162155 1104 64-65 -0.1822702116077437 1104 66-67 -0.18018021489816505 1104 68-69 -0.13696575083724127 1104 70-71 -0.26468715622440087 1104 72-73 -0.2725285771422037 1104 74-75 -0.37827050380125726 1104 76-77 -0.1541138358085412 1104 78-79 -0.18093196582391613 1104 80-81 -0.0034370002360795127 1104 82-83 0.21427238596713494 1104 84-85 0.09950195397783546 1104 86-87 0.04028367200378824 1104 88-89 0.11742153750534712 1104 90-91 -0.14031082720739008 1104 92-93 -0.05929600600307339 1104 94-95 -0.1863983366306421 1104 96-97 -0.14293061547958175 1104 98-99 -0.08154826769320067 1104 100-101 -0.27949665056357276 1105 1 0.3061256368408287 1105 2 0.3908247449840232 1105 3 0.18511022097720442 1105 4 0.13981350960705896 1105 5 0.041996462082053654 1105 6 0.06470952051876822 1105 7 0.11424951341076195 1105 8 -0.059745253043743674 1105 9 0.07524757482556765 1105 10-11 0.08359015851274165 1105 12-13 0.041272655633662225 1105 14-15 0.2860438646641228 1105 16-17 0.19372246678113214 1105 18-19 0.06484764189750791 1105 20-21 0.15696792423030814 1105 22-23 0.08071886760431113 1105 24-25 -0.09367521248537969 1105 26-27 -0.0408403386688434 1105 28-29 0.014979924886333151 1105 30-31 0.026409526170382946 1105 32-33 0.010137949841400484 1105 34-35 -0.1648415777793346 1105 36-37 0.017883833768784996 1105 38-39 -0.019032836929035568 1105 40-41 -0.10086980470916984 1105 42-43 0.0761132122854633 1105 44-45 -0.1270851242753679 1105 46-47 0.06914354997173433 1105 48-49 -0.00730391076937309 1105 50-51 -0.27876939685314284 1105 52-53 -0.3214223094977555 1105 54-55 -0.29691645934559574 1105 56-57 -0.3591969681798659 1105 58-59 -0.35364672365084004 1105 60-61 -0.28538883263068726 1105 62-63 -0.2896497919192029 1105 64-65 -0.15536118265418253 1105 66-67 -0.3502491113830608 1105 68-69 -0.33036523737157353 1105 70-71 -0.29158224057404425 1105 72-73 -0.3025038977286485 1105 74-75 -0.29917037191065887 1105 76-77 -0.11715378988769487 1105 78-79 -0.041684193537779635 1105 80-81 -0.15602798370589355 1105 82-83 -0.07323568288776983 1105 84-85 -0.16395877218614174 1105 86-87 -0.15461549329757318 1105 88-89 0.010883495865240178 1105 90-91 -0.010329117456969072 1105 92-93 0.2597185009486864 1105 94-95 0.3986482814479544 1105 96-97 0.27229633421690735 1105 98-99 0.26195296374209676 1105 100-101 0.1948057262561349 1106 1 0.3339458614847075 1106 2 0.2940397533776249 1106 3 0.09550595594585332 1106 4 0.023985170870467698 1106 5 -0.1391011162428768 1106 6 -0.05798042618508248 1106 7 -0.10653927860815315 1106 8 -0.0819524491683552 1106 9 -0.08049736906925631 1106 10-11 -0.13442670902570342 1106 12-13 -0.03440001793867964 1106 14-15 0.23575478895323698 1106 16-17 0.11497576606026882 1106 18-19 -0.12045147879683071 1106 20-21 0.094837986051175 1106 22-23 0.0261579541663437 1106 24-25 -0.025317440357270016 1106 26-27 -0.03938087314787708 1106 28-29 -0.0016873275749844652 1106 30-31 0.09026253746171164 1106 32-33 0.1456609835658753 1106 34-35 -0.24633063429847368 1106 36-37 -0.19415625466947262 1106 38-39 0.06299236116718276 1106 40-41 0.12052681899364615 1106 42-43 -0.18410764903350696 1106 44-45 -0.0313272259503492 1106 46-47 0.05407300634872314 1106 48-49 -0.04473005182760659 1106 50-51 6.842185319513305E-4 1106 52-53 0.31313276830972114 1106 54-55 0.023869989494727406 1106 56-57 -0.0685828489667486 1106 58-59 0.01955718811844065 1106 60-61 0.20308412346205174 1106 62-63 -0.09020512524472579 1106 64-65 0.03142362484532768 1106 66-67 -0.08769399787000154 1106 68-69 0.003258203787027014 1106 70-71 0.023728702014178538 1106 72-73 0.31160611004289507 1106 74-75 0.20884636731086204 1106 76-77 0.1695034259586663 1106 78-79 0.12109865461810898 1106 80-81 -0.11924147461240509 1106 82-83 0.19589902031239959 1106 84-85 0.45344646995067706 1106 86-87 0.5246142369523561 1106 88-89 0.2835206931558538 1106 90-91 0.2648582391976859 1106 92-93 0.4940586758056007 1106 94-95 0.20530452654889686 1106 96-97 0.16453773516991177 1106 98-99 0.25729589629704464 1106 100-101 0.07057039748307403 1107 1 0.49817009523163236 1107 2 0.46432326044199357 1107 3 0.3601651347689092 1107 4 0.21682542834775376 1107 5 0.06903432464992676 1107 6 0.2656604600862238 1107 7 0.09999390079335058 1107 8 0.12615749576834645 1107 9 0.2678602531460115 1107 10-11 0.3206808878927916 1107 12-13 0.3650909393627586 1107 14-15 0.5875288692862171 1107 16-17 0.6938789778336627 1107 18-19 0.5541663408049402 1107 20-21 0.4893476709291278 1107 22-23 0.5262129298216891 1107 24-25 0.5900768585876008 1107 26-27 0.25678971392041916 1107 28-29 0.33454696751361723 1107 30-31 0.3538568617431821 1107 32-33 0.366748768532986 1107 34-35 0.2746079196505704 1107 36-37 0.49830992932687224 1107 38-39 0.5157160861901176 1107 40-41 0.6688615745232767 1107 42-43 0.4202409253043342 1107 44-45 0.3540788091018072 1107 46-47 0.7147138631403251 1107 48-49 0.9243851827232348 1107 50-51 0.8412460537946131 1107 52-53 0.5167749453138057 1107 54-55 0.6230994538964012 1107 56-57 0.46583948039160106 1107 58-59 0.44411863775476235 1107 60-61 0.47789706680286415 1107 62-63 0.3010398821291851 1107 64-65 0.5334254557736777 1107 66-67 0.29341955394773933 1107 68-69 0.28381689986009206 1107 70-71 0.09265701933684412 1107 72-73 0.42899984967022675 1107 74-75 0.6244603298525213 1107 76-77 0.5897345468007344 1107 78-79 0.8646184988378209 1107 80-81 0.8356422359415667 1107 82-83 0.6522666751863895 1107 84-85 0.677929599196851 1107 86-87 0.81921323735153 1107 88-89 0.8051165647231606 1107 90-91 0.8586032844171569 1107 92-93 1.1697493174352402 1107 94-95 1.1349699478974564 1107 96-97 1.104296368140485 1107 98-99 1.4206763732467103 1107 100-101 1.103321546593783 1108 1 0.3773801876189502 1108 2 0.34698604279136447 1108 3 0.04031744366286816 1108 4 0.15632199242320866 1108 5 0.02563161535833558 1108 6 0.040101481317158516 1108 7 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-1.0164053406901843 2316 56-57 -0.9623423212775251 2316 58-59 -1.2697032967627635 2316 60-61 -1.351043150018917 2316 62-63 -1.321066634121273 2316 64-65 -0.917396323651591 2316 66-67 -1.2446353375365788 2316 68-69 -1.256249999398399 2316 70-71 -1.2725363639520317 2316 72-73 -1.43709544404798 2316 74-75 -1.3501506470471156 2316 76-77 -1.3066645181745429 2316 78-79 -1.3039751775894146 2316 80-81 -1.2592183417002794 2316 82-83 -1.2571496609195094 2316 84-85 -1.4361156122945076 2316 86-87 -1.4724405631774609 2316 88-89 -1.46186359324739 2316 90-91 -1.5932105266189858 2316 92-93 -1.5512648462611551 2316 94-95 -1.8577847282740834 2316 96-97 -2.0511822312226577 2316 98-99 -2.0604156439727213 2316 100-101 -2.725087025118224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 25.0 5 70.0 6 140.0 7 321.0 8 735.0 9 1265.0 10 2083.0 11 3341.0 12 4769.0 13 6550.0 14 8370.0 15 11438.0 16 15023.0 17 19085.0 18 15900.0 19 9901.0 20 8732.0 21 8998.0 22 9316.0 23 10487.0 24 12060.0 25 14161.0 26 16688.0 27 20395.0 28 24800.0 29 30305.0 30 36352.0 31 44562.0 32 55076.0 33 68576.0 34 89193.0 35 118450.0 36 153204.0 37 167662.0 38 82660.0 39 4062.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.87683153413768 19.182953503139775 15.324631341282721 18.61558362143983 2 15.966162132183939 24.227518810705583 36.10724940306368 23.69906965404679 3 23.472297822253857 25.908805426453963 30.464440071273387 20.154456680018797 4 12.467826854038602 17.27076807977265 38.669841642054095 31.591563424134648 5 11.803001389135712 41.039330311908884 32.71382036512031 14.443847933835086 6 28.770182243812393 38.06222546426255 15.76701598203893 17.400576309886123 7 26.74314201470269 35.88594386328597 19.675111582116717 17.69580253989462 8 27.05530211608262 30.06655383621969 18.945001204910078 23.933142842787614 9 25.834056779598725 18.751913294383545 21.497194145063308 33.91683578095443 10-11 26.26270567858756 26.00697547511527 25.181493079187973 22.5488257671092 12-13 30.283977565390856 24.57214022002888 25.378314203623482 19.765568010956784 14-15 23.944877431744604 26.020208072715196 28.94530310480815 21.089611390732053 16-17 20.14074652320638 29.123886156001895 28.6752316079083 22.06013571288342 18-19 19.857383307482817 28.741431879780677 31.434847860330915 19.966336952405587 20-21 22.730233194295703 24.29242934993473 30.82104154474124 22.156295911028323 22-23 21.09086307617228 25.35569751045471 31.693098284305858 21.860341129067148 24-25 23.273512726920856 25.37870928543715 31.406376626047084 19.94140136159491 26-27 22.02032344639799 23.906822725079866 34.25640033179238 19.81645349672976 28-29 19.775226118356596 28.45057868248898 31.96942784914712 19.804767350007303 30-31 20.863785613466707 25.276780405836046 31.64172952513517 22.217704455562078 32-33 20.975225536432664 26.08518254365923 31.95645731358671 20.983134606321393 34-35 22.982648267571548 24.804770912284848 31.747045627733744 20.465535192409863 36-37 22.341197121340905 24.12540190083802 33.42675158050369 20.10664939731739 38-39 21.16760136494637 25.998646218833375 32.862874993894025 19.970877422326225 40-41 20.909808643270505 26.79724635381944 31.555274138717927 20.737670864192133 42-43 20.414390320842628 27.29484040687152 29.598497728074264 22.692271544211593 44-45 21.941959228449182 25.12741400397955 30.04248916835583 22.88813759921544 46-47 23.62214468481042 27.117422656431728 29.50072109792975 19.7597115608281 48-49 23.03833441275655 26.929468998220813 28.977628023315543 21.054568565707097 50-51 20.198341217234667 27.818851394352624 31.305756426527125 20.677050961885584 52-53 22.3353916596611 28.92944855060361 27.50389512631189 21.231264663423403 54-55 20.460872118153443 27.079575279641073 28.9585827393728 23.500969862832687 56-57 23.098388417448266 26.361889608456156 28.227677906803635 22.312044067291943 58-59 25.0651079829505 25.913628526740563 27.541956151785573 21.479307338523363 60-61 21.87184064547256 27.11402545209738 30.913834791666954 20.100299110763103 62-63 21.327471958962317 27.080973221384742 30.175408560094052 21.41614625955889 64-65 20.665512081268915 29.81226664780616 28.56426282110937 20.957958449815553 66-67 22.029972206947054 26.882358250312404 27.574346649248323 23.51332289349222 68-69 21.286204957076723 27.506532814336882 28.413799648574827 22.793462580011564 70-71 23.519978711456968 27.455009576587813 27.502322619123493 21.522689092831726 72-73 22.631142133798054 25.978605635257434 30.028126974261447 21.362125256683065 74-75 20.822102237783188 30.42588686499145 27.63882285402724 21.113188043198118 76-77 21.928388496940006 27.17492085423921 27.553378320225725 23.343312328595054 78-79 21.998365039196717 27.82620469020657 28.170458389223963 22.004971881372754 80-81 23.15074433662981 27.387186261435854 27.758679894768285 21.703389507166047 82-83 22.288664993499964 26.729349930998158 29.413256356498035 21.568728719003843 84-85 21.222347119373346 28.290150763681975 28.53822876402069 21.94927335292399 86-87 21.382230328295986 28.657317799449118 27.23470185364401 22.72575001861088 88-89 20.986415657338203 28.612652876789717 26.10515009975006 24.295781366122025 90-91 22.12171948101745 26.637828098432088 27.021173825276623 24.21927859527384 92-93 23.148300209679498 28.277887908234167 27.44586923945473 21.127942642631606 94-95 21.991353552422577 27.85762207803205 27.401571286200948 22.749453083344424 96-97 19.53483697705451 28.089098241820004 29.70169378597737 22.674370995148116 98-99 21.381349832432957 29.144847435593817 25.964586428349097 23.50921630362413 100-101 20.25574776419615 27.780778856770365 27.06042684183662 24.903046537196865 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1228.0 1 878.5 2 736.5 3 980.0 4 850.5 5 753.0 6 1240.0 7 2550.0 8 3828.5 9 4282.5 10 3716.0 11 2896.0 12 2755.5 13 2810.0 14 2910.0 15 2979.0 16 2994.0 17 3139.0 18 3306.0 19 3402.5 20 3633.5 21 3960.5 22 4363.0 23 4957.0 24 5569.0 25 6334.0 26 7479.0 27 8703.5 28 9737.5 29 11183.0 30 13032.5 31 14706.0 32 17157.0 33 18706.0 34 19177.0 35 19877.0 36 21194.5 37 22598.5 38 23214.0 39 23242.0 40 23247.5 41 24599.0 42 25589.5 43 26126.0 44 27053.0 45 28791.5 46 31523.0 47 51705.0 48 94505.0 49 89099.5 50 46880.5 51 30505.5 52 26193.0 53 25535.0 54 25154.5 55 24098.0 56 22484.0 57 20873.5 58 20517.5 59 19265.5 60 16499.0 61 14220.5 62 12505.0 63 9735.0 64 7064.0 65 5578.0 66 4134.5 67 3195.5 68 2442.0 69 1751.5 70 1323.5 71 1063.0 72 959.0 73 939.0 74 763.5 75 640.5 76 522.0 77 355.0 78 259.5 79 194.0 80 139.0 81 104.5 82 77.0 83 54.0 84 32.5 85 12.5 86 5.5 87 4.0 88 2.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0973520821683542 2 0.01069997366876045 3 0.0031634704759813505 4 0.01069997366876045 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004838248963265595 10-11 3.2565137252749197E-4 12-13 9.304324929356913E-5 14-15 4.6521624646784564E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013491271147567524 24-25 0.0 26-27 4.6521624646784564E-5 28-29 0.0 30-31 0.0 32-33 0.004977813837205949 34-35 5.582594957614147E-4 36-37 0.003023905602040997 38-39 1.3956487394035369E-4 40-41 0.004512597590738103 42-43 0.012095622408163987 44-45 0.0022795596076924437 46-47 0.0017678217365778134 48-49 0.011258233164521866 50-51 4.6521624646784566E-4 52-53 0.00265173260486672 54-55 0.012328230531397909 56-57 0.004559119215384887 58-59 0.001488691988697106 60-61 0.007769111316013022 62-63 0.004605640840031672 64-65 0.019818212099530226 66-67 0.0037217299717427653 68-69 2.3260812323392283E-4 70-71 0.0011630406161696142 72-73 0.0 74-75 0.0 76-77 0.001069997366876045 78-79 0.011676927786342925 80-81 0.017492130867190998 82-83 0.014886919886971061 84-85 0.009955627674411898 86-87 0.012002579158870418 88-89 0.031029923639405304 90-91 0.02623819630078649 92-93 0.003116948851334566 94-95 0.010048670923705467 96-97 0.012560838654631833 98-99 0.011583884537049356 100-101 0.014468225265149999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1074769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.182262917755324 #Duplication Level Percentage of deduplicated Percentage of total 1 74.84803168544599 32.3210738311741 2 13.493651501770037 11.653728137399954 3 4.890903367107863 6.3360082531135955 4 2.184773654671411 3.773738814872242 5 1.2175689451442064 2.6288691154855566 6 0.7335763975726645 1.900649332214556 7 0.4792526623574316 1.448665012896698 8 0.34780025977988926 1.2015041808543026 9 0.2521427498332626 0.9799285064495178 >10 1.3536001692320165 10.754887110974082 >50 0.11744476128863561 3.4770523507500215 >100 0.06673649207431728 5.412138026327117 >500 0.006716984558081247 2.051153803924113 >1k 0.0049835691882538285 4.774205038099985 >5k 0.0021667692122842733 6.5282867989870095 >10k+ 6.500307636852819E-4 4.7581116864771875 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 29506 2.745334113656051 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11135 1.0360365808838923 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10212 0.9501576617859279 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9914 0.9224307734964443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9453 0.879537835572109 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7194 0.6693531354179363 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6532 0.6077585043855935 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6521 0.6067350286433644 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6368 0.5924994115014482 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6225 0.5791942268524679 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6143 0.5715646804103951 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5988 0.5571429767698919 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5434 0.5055970166612547 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4728 0.4399084826599948 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4273 0.3975738042314209 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3227 0.30025056547034756 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3057 0.28443321309044084 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2932 0.2728028069287447 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2868 0.26684803897395626 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2718 0.2528915515799209 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2478 0.2305611717494643 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2426 0.2257229227861987 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2372 0.22069858732434597 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2359 0.21948902508352958 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2159 0.20088037522481575 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2011 0.18710997432936752 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1944 0.18087607662669838 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1880 0.17492130867190997 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1411 0.13128402475322604 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1311 0.12197969982386914 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1265 0.11769971035636495 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1229 0.11435015338179645 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1213 0.11286146139309935 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.304324929356913E-5 0.0 24-25 0.0 0.0 0.0 1.8608649858713826E-4 0.0 26-27 0.0 0.0 0.0 5.117378711146303E-4 0.0 28-29 0.0 0.0 0.0 0.0013026054901099679 0.0 30-31 0.0 0.0 0.0 0.002558689355573151 0.0 32-33 0.0 0.0 0.0 0.005629116582260932 0.0 34-35 0.0 0.0 0.0 0.009629976301884405 0.0 36-37 0.0 0.0 0.0 0.014096052267975723 0.0 38-39 0.0 0.0 0.0 0.024005158317740836 0.0 40-41 0.0 0.0 0.0 0.035356434731556266 0.0 42-43 0.0 0.0 0.0 0.05317421697127476 0.0 44-45 0.0 0.0 0.0 0.06889852610188794 0.0 46-47 0.0 0.0 0.0 0.09923062537159147 0.0 48-49 0.0 0.0 0.0 0.13435445197991383 0.0 50-51 0.0 0.0 0.0 0.15640570206248972 0.0 52-53 0.0 0.0 0.0 0.18701693108007394 0.0 54-55 0.0 0.0 0.0 0.2356785504606106 0.0 56-57 0.0 0.0 0.0 0.3125787960017455 0.0 58-59 0.0 0.0 0.0 0.4026911829425672 0.0 60-61 0.0 0.0 0.0 0.5036431084260897 0.0 62-63 0.0 0.0 0.0 0.6115267559819831 0.0 64-65 0.0 0.0 0.0 0.7235043065067935 0.0 66-67 0.0 0.0 0.0 0.8634878750689683 0.0 68-69 0.0 0.0 0.0 1.0387348351134058 0.0 70-71 0.0 0.0 0.0 1.178811446924874 0.0 72-73 0.0 0.0 0.0 1.3383340978386984 0.0 74-75 0.0 0.0 0.0 1.5113014982754434 0.0 76-77 0.0 0.0 0.0 1.6946432210084215 0.0 78-79 0.0 0.0 0.0 1.9252509143825325 0.0 80-81 0.0 0.0 0.0 2.2238732229902425 0.0 82-83 0.0 0.0 0.0 2.51342381479183 0.0 84-85 0.0 0.0 0.0 2.868569897345383 0.0 86-87 0.0 0.0 0.0 3.266329788075391 0.0 88-89 0.0 0.0 0.0 3.708796960090959 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATAACG 60 0.0039472105 31.672611 2 GTAAGGT 465 0.0 31.668186 4 GGTAAGG 465 0.0 30.650911 3 AGGTAAG 460 0.0 29.951271 2 ATTATCG 65 0.005846521 29.228094 6 AAGGTAA 515 0.0 28.911133 1 TAAGGTG 540 0.0 27.26602 5 CGGTTAC 120 9.094947E-12 25.725016 28-29 GTACATG 8625 0.0 24.16803 1 GGTGTGC 805 0.0 23.600323 8 TACATGG 8690 0.0 23.453768 2 GAGTACT 8730 0.0 22.92748 12-13 GTGTGCA 770 0.0 22.822584 9 AAGGTGT 730 0.0 22.771887 6 ACATGGG 8615 0.0 22.665308 3 GGGCGTA 150 3.0351224E-5 22.164637 9 CATGGGA 5755 0.0 21.955875 4 AGGTGTG 785 0.0 21.781445 7 GTACTTT 9205 0.0 21.718573 14-15 AGTACTT 8790 0.0 21.663494 12-13 >>END_MODULE