##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936381_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1114777 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.740932939951218 31.0 31.0 34.0 30.0 34.0 2 31.76394292311377 33.0 31.0 34.0 30.0 34.0 3 31.795140193958073 33.0 31.0 34.0 30.0 34.0 4 35.42814571882987 37.0 35.0 37.0 33.0 37.0 5 35.427019036094215 37.0 35.0 37.0 33.0 37.0 6 35.59258667877073 37.0 35.0 37.0 33.0 37.0 7 35.488708504032644 37.0 35.0 37.0 33.0 37.0 8 35.45295606206443 37.0 35.0 37.0 33.0 37.0 9 36.96477053258185 39.0 37.0 39.0 33.0 39.0 10-11 36.884589922468805 39.0 37.0 39.0 33.0 39.0 12-13 36.80659584831764 39.0 37.0 39.0 33.0 39.0 14-15 37.789291490585114 40.0 37.0 41.0 33.0 41.0 16-17 37.737894215614425 40.0 37.0 41.0 32.5 41.0 18-19 37.7985920053966 40.0 37.0 41.0 33.0 41.0 20-21 37.73158174235744 40.0 37.0 41.0 32.5 41.0 22-23 37.6031923873564 40.0 37.0 41.0 32.0 41.0 24-25 37.68171033309801 40.0 37.0 41.0 32.0 41.0 26-27 37.61296923061742 40.0 37.0 41.0 32.5 41.0 28-29 37.53081019791402 40.0 37.0 41.0 32.0 41.0 30-31 37.32093862718732 39.0 36.5 41.0 32.0 41.0 32-33 37.03374217444386 39.0 35.5 41.0 31.5 41.0 34-35 36.726021437471346 39.0 35.0 41.0 31.0 41.0 36-37 36.47521836205806 39.0 35.0 40.0 30.5 41.0 38-39 36.22779623189212 38.0 35.0 40.0 30.0 41.0 40-41 36.06411865332708 38.0 35.0 40.0 30.0 41.0 42-43 35.74272791778087 38.0 35.0 40.0 29.5 41.0 44-45 35.344233869195364 38.0 35.0 40.0 27.5 41.0 46-47 34.822152771361445 38.0 34.0 40.0 25.0 41.0 48-49 34.50685069749376 38.0 34.0 40.0 23.0 41.0 50-51 33.48014759902654 37.5 33.0 39.5 22.0 40.5 52-53 33.63532213169091 37.5 33.0 39.5 22.0 40.5 54-55 34.052217618411575 38.0 34.0 40.0 22.0 41.0 56-57 33.86024873136063 38.0 33.5 40.0 21.0 41.0 58-59 33.58376877169155 37.5 33.0 40.0 19.5 41.0 60-61 33.14139599220292 37.0 33.0 40.0 18.0 41.0 62-63 32.87675696574293 36.0 33.0 40.0 16.0 41.0 64-65 32.53695178497583 36.0 32.0 39.0 13.0 41.0 66-67 32.048338367225014 35.5 31.5 39.0 8.0 40.5 68-69 31.611977103940966 35.0 31.0 38.5 7.0 40.0 70-71 31.06565528352307 35.0 30.5 37.5 4.0 40.0 72-73 30.670119674159046 35.0 30.5 37.0 2.0 39.0 74-75 30.28862768069309 35.0 30.0 36.5 2.0 39.0 76-77 29.87035568548687 34.0 29.5 36.0 2.0 39.0 78-79 29.398489563383528 34.0 29.0 35.5 2.0 37.5 80-81 28.861842772141873 34.0 29.0 35.0 2.0 37.0 82-83 28.351623687966292 34.0 27.5 35.0 2.0 36.5 84-85 28.021722730196263 34.0 27.0 35.0 2.0 36.0 86-87 27.824833576580787 34.0 27.0 35.0 2.0 36.0 88-89 27.356347502684393 33.5 26.0 35.0 2.0 35.0 90-91 26.8607196775678 33.0 25.0 35.0 2.0 35.0 92-93 26.486485189414566 33.0 25.0 35.0 2.0 35.0 94-95 26.008192221403924 33.0 23.5 35.0 2.0 35.0 96-97 25.4009313073377 32.5 19.5 35.0 2.0 35.0 98-99 24.773608533365866 32.0 10.0 35.0 2.0 35.0 100-101 22.42255850273194 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37461354657837376 1101 2 0.3503821754391403 1101 3 0.46218736336800603 1101 4 0.47679376760758885 1101 5 0.4561295138258359 1101 6 0.33258512558231956 1101 7 0.34210838380444386 1101 8 0.3910585267002631 1101 9 0.6671840788363568 1101 10-11 0.5648930605211788 1101 12-13 0.607221268081112 1101 14-15 0.785976913978395 1101 16-17 0.8449691656602738 1101 18-19 0.877319935273313 1101 20-21 0.9581065991730355 1101 22-23 0.8641803612998302 1101 24-25 0.8105206641772185 1101 26-27 0.7034635812954662 1101 28-29 0.8255949287531976 1101 30-31 0.785974222221121 1101 32-33 0.7692639459683406 1101 34-35 0.8339750071336951 1101 36-37 0.8043560195418635 1101 38-39 0.79579896773717 1101 40-41 0.7780698742750545 1101 42-43 0.8257898443056817 1101 44-45 0.8987571510016323 1101 46-47 0.8802057836542545 1101 48-49 1.034462092317625 1101 50-51 1.129332095693151 1101 52-53 1.1820100270931988 1101 54-55 1.1849793344296273 1101 56-57 1.0327396594389882 1101 58-59 1.0638057770628606 1101 60-61 1.0255550503945372 1101 62-63 1.093669799041308 1101 64-65 1.0672341022861858 1101 66-67 1.2259746976060306 1101 68-69 1.3465411354101064 1101 70-71 1.3599203336827514 1101 72-73 1.0956994983153692 1101 74-75 1.0719717411703833 1101 76-77 1.1039038257469507 1101 78-79 1.0345044824467315 1101 80-81 1.0940997123807072 1101 82-83 1.192842688644955 1101 84-85 1.290345988119931 1101 86-87 1.0936317614948408 1101 88-89 1.041061364077244 1101 90-91 0.9867267653043506 1101 92-93 1.1407885900154184 1101 94-95 1.1505570551163977 1101 96-97 1.0914614025610447 1101 98-99 0.8412088910584252 1101 100-101 1.3213751893656678 1102 1 0.09809368239338667 1102 2 0.1855800737761477 1102 3 0.08314203386789742 1102 4 0.1302168181581962 1102 5 0.16981841152672672 1102 6 0.157236707199381 1102 7 0.19776362515698054 1102 8 0.125373296996095 1102 9 0.24538758656476034 1102 10-11 0.21696663524835458 1102 12-13 0.24885592238894105 1102 14-15 0.32331406401103635 1102 16-17 0.21543683524316037 1102 18-19 0.38481165336628465 1102 20-21 0.4230338588325111 1102 22-23 0.16270035766488178 1102 24-25 0.04991569255462025 1102 26-27 0.1482251202018432 1102 28-29 0.188730735505942 1102 30-31 -0.08208983554155935 1102 32-33 -0.09740891663874862 1102 34-35 -0.4369503359148723 1102 36-37 -0.10201960315014702 1102 38-39 -0.2299426863310856 1102 40-41 -0.293311903051098 1102 42-43 -0.13283738313490545 1102 44-45 -0.13411107373752174 1102 46-47 -0.08170157464458327 1102 48-49 0.08885831857684678 1102 50-51 0.061089181783678725 1102 52-53 0.10243639040662345 1102 54-55 0.10542511168970492 1102 56-57 0.16293949917066186 1102 58-59 0.39071226793004854 1102 60-61 0.2972098621618713 1102 62-63 0.19840762814900614 1102 64-65 0.07394017513393436 1102 66-67 0.20078448223513945 1102 68-69 0.03365192708606912 1102 70-71 0.1090339011400232 1102 72-73 0.18306597579932316 1102 74-75 0.04447457064984306 1102 76-77 0.2602385552321884 1102 78-79 0.1042641625709777 1102 80-81 0.3497264524073067 1102 82-83 0.4017693865391614 1102 84-85 0.25179154232614565 1102 86-87 0.12835587692108064 1102 88-89 0.11110729664888908 1102 90-91 0.27688967723112157 1102 92-93 0.017752909659851213 1102 94-95 0.0713584793565687 1102 96-97 0.04125858877796773 1102 98-99 0.17133685788122932 1102 100-101 0.3203264231839391 1103 1 0.1476119936672866 1103 2 0.2003427298367022 1103 3 0.14980227463616202 1103 4 0.30666945167737936 1103 5 0.3965816019514605 1103 6 0.30674368859978784 1103 7 0.28490810810044565 1103 8 0.3383317975296194 1103 9 0.5332027303780578 1103 10-11 0.4456415304470269 1103 12-13 0.31495136050838113 1103 14-15 0.2700209601837358 1103 16-17 0.33829663059520954 1103 18-19 0.35277812708689993 1103 20-21 0.3885648325083082 1103 22-23 0.3342735359649609 1103 24-25 0.512404087889422 1103 26-27 0.4053075594052373 1103 28-29 0.09837372611905693 1103 30-31 0.13259435691747257 1103 32-33 0.2557897433923344 1103 34-35 0.21175467645498003 1103 36-37 0.11971447626380893 1103 38-39 0.1955249246044417 1103 40-41 0.14753939320479503 1103 42-43 0.2859007902527466 1103 44-45 0.4647708199409166 1103 46-47 0.36927727569455016 1103 48-49 0.241103070900742 1103 50-51 0.36004776348644185 1103 52-53 0.2991339859369049 1103 54-55 0.474739671253559 1103 56-57 0.5087513353373083 1103 58-59 0.3046614559839682 1103 60-61 0.04879266239928626 1103 62-63 0.24798639359864438 1103 64-65 0.4072012171102628 1103 66-67 0.3192332365409136 1103 68-69 0.29697171530198574 1103 70-71 0.41129843846688274 1103 72-73 0.5302750221114501 1103 74-75 0.523954816930857 1103 76-77 0.5072949019060253 1103 78-79 0.4461849481539666 1103 80-81 0.3992857710591551 1103 82-83 0.2695780875438025 1103 84-85 0.2982097803786239 1103 86-87 0.33193510232975854 1103 88-89 0.27970891969554046 1103 90-91 0.4214334976306411 1103 92-93 0.4079250053651542 1103 94-95 0.3299556550051186 1103 96-97 0.31410857617982657 1103 98-99 0.5884165576226721 1103 100-101 1.314944310517646 1104 1 -0.14568565227391872 1104 2 -6.544768523859545E-4 1104 3 0.005925534123957021 1104 4 0.1609263761341495 1104 5 0.1493063626448503 1104 6 0.07068414985874227 1104 7 0.11280902074712174 1104 8 -0.026413752897695986 1104 9 0.08888939130124385 1104 10-11 0.11228003992641078 1104 12-13 0.020103152809177516 1104 14-15 0.10883727920495545 1104 16-17 0.19167080511788015 1104 18-19 0.31836989681476524 1104 20-21 0.3185958437434735 1104 22-23 0.10547014861110426 1104 24-25 0.17687375684935347 1104 26-27 -0.12375729175676042 1104 28-29 0.1881099448712149 1104 30-31 0.06632752231013939 1104 32-33 -0.1497445149119656 1104 34-35 -0.17357539948981326 1104 36-37 -0.39700558586480383 1104 38-39 -0.09159656761444523 1104 40-41 -0.01667686106964794 1104 42-43 0.08641434196791664 1104 44-45 0.04928174286570197 1104 46-47 -0.13779843382233992 1104 48-49 -0.19837416069640312 1104 50-51 -0.3005994567357888 1104 52-53 -0.0486898320361675 1104 54-55 0.08591530310968665 1104 56-57 -0.11433965159127268 1104 58-59 -0.1416937951398225 1104 60-61 -0.18641420005270248 1104 62-63 0.12426076430762123 1104 64-65 -0.27637140808896987 1104 66-67 -0.29687270406540733 1104 68-69 -0.314769566436766 1104 70-71 -0.22985531038987617 1104 72-73 -0.12272862741382795 1104 74-75 -0.3161475926757298 1104 76-77 -0.2228905463416524 1104 78-79 -0.2206645191002572 1104 80-81 -0.234768828527951 1104 82-83 -0.21959584249782438 1104 84-85 -0.01822378605493924 1104 86-87 -0.005993159643530532 1104 88-89 0.14478881829428758 1104 90-91 0.26685363529717776 1104 92-93 0.20191434514250162 1104 94-95 0.17013634454072601 1104 96-97 0.28070294816251007 1104 98-99 0.6067521936537261 1104 100-101 0.92247882002534 1105 1 0.29259385925932335 1105 2 0.2777498650743446 1105 3 0.3039499574618141 1105 4 0.3053334317650851 1105 5 0.3097948294019801 1105 6 0.4158184782169485 1105 7 0.3997181116562132 1105 8 0.44376263787571446 1105 9 0.4574701240013823 1105 10-11 0.37519184453970666 1105 12-13 0.3708738448037536 1105 14-15 0.21003945016832404 1105 16-17 0.33688925830647776 1105 18-19 0.4048312265298293 1105 20-21 0.4409242019524271 1105 22-23 0.24808887317012562 1105 24-25 0.46481134165260585 1105 26-27 0.462031039315157 1105 28-29 0.340823649077457 1105 30-31 0.3791253513467794 1105 32-33 0.17683893149237662 1105 34-35 0.20063626943827018 1105 36-37 0.4682888510415708 1105 38-39 0.4817792804181167 1105 40-41 0.30954294897106394 1105 42-43 0.35570877887429475 1105 44-45 0.12646763160383045 1105 46-47 0.5479220546444239 1105 48-49 0.5771997877432185 1105 50-51 0.5358265948245915 1105 52-53 0.46339972020263787 1105 54-55 0.3524037698668039 1105 56-57 0.4444744596706016 1105 58-59 0.48391000404607354 1105 60-61 0.2747988257274798 1105 62-63 0.23236117136325163 1105 64-65 0.4206353761715391 1105 66-67 0.6717812986482947 1105 68-69 0.6420825366839935 1105 70-71 0.756036141441875 1105 72-73 0.8676404363582222 1105 74-75 0.8086828008113791 1105 76-77 0.6636386259785674 1105 78-79 0.7509989815781708 1105 80-81 0.704227100915741 1105 82-83 0.869041877990643 1105 84-85 0.9428671230359669 1105 86-87 0.8060394048069419 1105 88-89 0.6973369865439487 1105 90-91 0.8320666610773664 1105 92-93 0.8946171945319215 1105 94-95 0.7513955032924251 1105 96-97 0.7964098681082348 1105 98-99 0.8837589779142405 1105 100-101 1.0891192542180157 1106 1 0.12813313965106232 1106 2 0.20105466955947549 1106 3 0.21048661206404162 1106 4 0.04903480143367034 1106 5 0.238327935640001 1106 6 0.23946818370271927 1106 7 0.2701080059149561 1106 8 0.24793835287109545 1106 9 0.3652841897170873 1106 10-11 0.3973415855091247 1106 12-13 0.49133200958978307 1106 14-15 0.4535176986957552 1106 16-17 0.5842763848411536 1106 18-19 0.448639614862806 1106 20-21 0.45547902417938246 1106 22-23 0.40932796195020416 1106 24-25 0.33034395111964443 1106 26-27 0.18922087680007138 1106 28-29 0.41910766014834877 1106 30-31 0.35682203717460936 1106 32-33 0.29921094199553266 1106 34-35 0.09711685988607144 1106 36-37 -0.04244111844248977 1106 38-39 0.06774166775755219 1106 40-41 0.04689075963035094 1106 42-43 -0.08996292646953918 1106 44-45 0.14640603200125213 1106 46-47 0.19085953246713672 1106 48-49 0.21267179114964563 1106 50-51 0.17015857977952464 1106 52-53 0.19178095645272464 1106 54-55 0.18148360806139863 1106 56-57 0.09195668236593946 1106 58-59 0.17837739457903723 1106 60-61 0.1350037792439096 1106 62-63 0.22802790899719838 1106 64-65 0.13908034104273526 1106 66-67 0.2584349073346921 1106 68-69 0.44020900038422894 1106 70-71 0.41790996161077487 1106 72-73 0.5146891908823861 1106 74-75 0.1808352385375862 1106 76-77 0.21768759340458033 1106 78-79 0.39355891344957783 1106 80-81 0.31857526641144673 1106 82-83 0.39469882213938945 1106 84-85 0.36538986805051366 1106 86-87 0.36623924867891944 1106 88-89 0.41762624919386937 1106 90-91 0.3691601249905041 1106 92-93 0.4988968056312011 1106 94-95 0.2973621486689062 1106 96-97 0.5857102726217533 1106 98-99 0.6644933473699233 1106 100-101 1.051751288850049 1107 1 0.2616262262731155 1107 2 0.3184630651869256 1107 3 0.2159015476672721 1107 4 0.33838099720274073 1107 5 0.42559274665500624 1107 6 0.36698200199966635 1107 7 0.3883508164127534 1107 8 0.4480885596319766 1107 9 0.43725171702136123 1107 10-11 0.360428545918829 1107 12-13 0.28132501283077005 1107 14-15 0.17365217181200165 1107 16-17 0.2453063706059453 1107 18-19 0.5131149304420148 1107 20-21 0.291757582194478 1107 22-23 0.4241471337949534 1107 24-25 0.5320460728656684 1107 26-27 0.360794337936035 1107 28-29 0.4359334154720571 1107 30-31 0.38263421709402934 1107 32-33 0.43023099453768765 1107 34-35 0.4604347495958834 1107 36-37 0.597962929285309 1107 38-39 0.5424865700325299 1107 40-41 0.27716721015878676 1107 42-43 0.4446674053871007 1107 44-45 0.38477497750869816 1107 46-47 0.6783979904732931 1107 48-49 0.6946886703776087 1107 50-51 0.8543956913627326 1107 52-53 0.7533195062988938 1107 54-55 0.824768532039613 1107 56-57 0.8455864776835469 1107 58-59 0.8957929198918393 1107 60-61 1.0281506410947756 1107 62-63 0.9805725420359224 1107 64-65 0.9847963668836144 1107 66-67 0.9565020979728125 1107 68-69 1.01692773792238 1107 70-71 0.9619218718105742 1107 72-73 0.9079759274924726 1107 74-75 0.9195398230461116 1107 76-77 0.8453884148893458 1107 78-79 0.9707135889522185 1107 80-81 1.0264871628352417 1107 82-83 1.012802362088589 1107 84-85 1.086582011862312 1107 86-87 1.1222251633208735 1107 88-89 0.9955618669267245 1107 90-91 0.9746988029293213 1107 92-93 1.0226869947007948 1107 94-95 1.3129429280039346 1107 96-97 1.2518787676297656 1107 98-99 1.2667378689922053 1107 100-101 1.7581127808152566 1108 1 0.05225819492365957 1108 2 0.124071543396024 1108 3 0.22324366375551818 1108 4 0.19752224295389453 1108 5 0.24392070352784856 1108 6 0.1702170796155471 1108 7 0.12417265711075487 1108 8 0.24075564486872025 1108 9 0.2640015925328498 1108 10-11 -0.011252211404347179 1108 12-13 0.28877943920934257 1108 14-15 0.2721618277907041 1108 16-17 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-1.6039113336963915 2316 92-93 -1.8786844146002473 2316 94-95 -1.68569760632111 2316 96-97 -2.9073596271468887 2316 98-99 -4.377447458614434 2316 100-101 -5.294873594083459 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1946.0 3 793.0 4 674.0 5 774.0 6 866.0 7 987.0 8 1254.0 9 1573.0 10 2147.0 11 3095.0 12 4432.0 13 5988.0 14 7057.0 15 9386.0 16 12851.0 17 18047.0 18 14382.0 19 8445.0 20 7900.0 21 8233.0 22 8713.0 23 9881.0 24 11099.0 25 12568.0 26 14595.0 27 16952.0 28 20479.0 29 24649.0 30 30585.0 31 37862.0 32 48400.0 33 63348.0 34 85587.0 35 119146.0 36 156551.0 37 186398.0 38 135526.0 39 21602.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.96153235264822 18.07292054584073 14.496348229660864 19.469198871850185 2 15.368588794556096 22.055569612757 37.8098042699198 24.766037322767104 3 22.578555522258966 26.009230531814293 30.306344555080827 21.10586939084591 4 11.797043356977262 16.035538594234808 39.43997723864153 32.7274408101464 5 11.831078200381464 39.96068663749579 33.31969033995288 14.888544822169864 6 28.78639382516604 36.47415185783522 17.08571172141447 17.653742595584276 7 26.307792139021547 35.548790283857194 20.362829578876795 17.78058799824446 8 26.5910282091599 29.42917555158277 19.894404118293235 24.085392120964094 9 26.09349619033089 18.097409066025293 22.489791522700962 33.31930322094286 10-11 25.654525447734 25.442079546494867 25.578414706605656 23.324980299165475 12-13 29.46378530411765 24.1922810966337 25.89465966114868 20.449273938099978 14-15 24.338386695987445 25.04593524151729 29.060596828348245 21.555081234147014 16-17 20.372832633221343 28.883983642464077 28.608815173575568 22.134368550739012 18-19 20.034626641691446 28.273487642381646 31.246280867120035 20.44560484880687 20-21 22.72356994186464 24.588521854589825 30.046113543386205 22.641794660159334 22-23 21.08927307326868 25.123074479911068 31.355034995590223 22.432617451230026 24-25 22.967478307284132 25.015710258428705 30.882398455213988 21.134412979073172 26-27 21.798456914726597 23.83084376261607 33.73377293320773 20.636926389449602 28-29 19.98141218085266 27.722314847334527 31.720983557584788 20.575289414228024 30-31 20.491520907737897 25.39026790576334 31.326753884288177 22.79145730221058 32-33 20.737670101400372 25.76386680475573 31.743245843535643 21.755217250308256 34-35 22.88071883404773 24.879877347505236 30.977784037592382 21.26161978085466 36-37 21.96897383266109 24.192318457806422 33.02751840900282 20.811189300529662 38-39 21.41596993173276 25.653257687185416 32.06465448989763 20.8661178911842 40-41 20.913640194463294 26.71075984482766 31.50237203417236 20.873227926536686 42-43 20.412611027809263 27.028058644302398 29.2685519547129 23.29077837317544 44-45 21.17893010715793 25.325306468273567 29.864862864670442 23.63090055989806 46-47 23.1416861175449 26.720662663463624 29.543746086650746 20.593905132340726 48-49 22.428264189618012 26.407793553364268 29.164825849336296 21.99911640768142 50-51 19.99156780516248 27.366464084680764 31.276356215379785 21.365611894776972 52-53 21.815391115690154 28.41002008987742 27.757356981288474 22.017231813143955 54-55 20.244808505904686 26.530714770146087 29.36177007790381 23.862706646045424 56-57 22.499923739868617 26.136859123313528 28.07139922089061 23.291817915927243 58-59 24.214049193078843 25.919667632274695 27.7374718414166 22.128811333229866 60-61 22.055561561300237 26.42423992317033 30.852577010218685 20.66762150531075 62-63 21.234635237717608 27.05382309195264 29.976559262096696 21.734982408233055 64-65 20.531415700180393 29.21696446912701 28.678112304075164 21.57350752661743 66-67 21.540718785000994 26.98141787861403 27.515328108312502 23.962535228072472 68-69 20.829041716311853 27.111924175650653 28.821049439428087 23.237984668609407 70-71 22.998940973989946 26.92940320004378 27.538892629295592 22.532763196670686 72-73 22.023243787057094 25.857872104898906 30.193644401724335 21.925239706319672 74-75 20.596720499702624 29.65432391143071 28.068128707693162 21.68082688117351 76-77 21.276919274584113 27.034574820007386 27.899965775498934 23.788540129909567 78-79 21.578540369801214 27.28219164318516 28.743562209621004 22.395705777392624 80-81 22.828109923738086 27.2030883361571 27.72742544602746 22.241376294077355 82-83 21.793067255160533 26.53820276125629 29.536471369235045 22.132258614348128 84-85 21.32561273432299 27.776302402489154 28.5027552633515 22.39532959983635 86-87 21.330994336709658 28.391986170555377 27.832744098758166 22.444275393976802 88-89 20.981610006912117 28.075117328774894 26.460436553200633 24.48283611111236 90-91 21.33196259904856 26.605611578039905 27.481203985535522 24.581221837376013 92-93 22.726636532798842 27.975240942344303 27.785618285599444 21.512504239257407 94-95 21.7388905485893 27.174588820437464 27.932085059197476 23.154435571775764 96-97 19.44199728709432 27.533929263496347 29.94320302295204 23.080870426457288 98-99 21.104962015276435 28.68517317211696 26.619139778578717 23.590725034027887 100-101 20.52944861030845 27.01454123846411 27.793696403636925 24.66231374759052 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 535.0 1 436.0 2 554.5 3 801.0 4 706.5 5 682.0 6 1191.5 7 2380.0 8 3780.5 9 4397.0 10 3642.0 11 2764.5 12 2705.5 13 2815.5 14 2900.5 15 2926.0 16 2909.5 17 2956.0 18 3078.0 19 3201.0 20 3417.0 21 3730.0 22 4224.0 23 4836.0 24 5329.5 25 6215.5 26 7513.5 27 8749.0 28 9930.0 29 11380.0 30 13073.5 31 14884.5 32 17606.5 33 19182.0 34 19745.0 35 20571.5 36 21772.5 37 23409.0 38 23985.0 39 23841.0 40 24185.5 41 25562.0 42 26658.5 43 27407.0 44 28518.5 45 30878.5 46 34271.0 47 53366.5 48 95548.5 49 91767.0 50 50245.5 51 33314.5 52 28042.5 53 26875.5 54 26690.5 55 25474.0 56 23762.5 57 22149.0 58 21479.0 59 19880.0 60 17303.5 61 15334.0 62 13578.0 63 10469.0 64 7440.0 65 6014.5 66 4516.0 67 3437.5 68 2630.0 69 1860.0 70 1411.0 71 1153.0 72 991.0 73 970.0 74 814.0 75 664.0 76 509.0 77 326.5 78 223.5 79 164.5 80 127.0 81 84.0 82 57.5 83 44.0 84 27.5 85 14.0 86 10.5 87 9.0 88 6.0 89 3.5 90 2.5 91 5.0 92 2.5 93 1.0 94 2.5 95 2.0 96 1.0 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034625759232564 2 0.025386243167916094 3 0.019286368484459224 4 0.05454005599326143 5 0.05848703372961588 6 0.0517592307699208 7 0.05292538328293461 8 0.04745343687571595 9 0.04547994800753873 10-11 0.05480916811164923 12-13 0.04597332022458303 14-15 0.03915581322542535 16-17 0.049382073724161875 18-19 0.05436064791433623 20-21 0.012289453406376343 22-23 0.018658440208221015 24-25 0.005337390348024762 26-27 0.011392413011750332 28-29 0.006144726703188171 30-31 0.008432179709484499 32-33 0.0033639014798475387 34-35 0.007445435275395886 36-37 0.00941892414357311 38-39 0.010181408479005217 40-41 0.0014801166511329172 42-43 0.0020183408879085236 44-45 0.0018389328089833215 46-47 2.691121183878031E-4 48-49 8.521883748947098E-4 50-51 1.7940807892520208E-4 52-53 0.003274197440384938 54-55 0.006189578722919472 56-57 0.01533939074810478 58-59 0.009688036261960912 60-61 0.009284368084379208 62-63 0.004574906012592653 64-65 0.0 66-67 0.006862359018888979 68-69 0.01803051193198281 70-71 0.006593246900501177 72-73 0.0024668610852215285 74-75 0.0030499373417284354 76-77 0.007041767097814182 78-79 0.006279282762382073 80-81 0.011796081189332038 82-83 0.00601017064399427 84-85 0.01641583922165599 86-87 0.004844018130980456 88-89 0.00605502266372557 90-91 0.004126385815279647 92-93 0.017492287695207203 94-95 0.010585076656586923 96-97 0.00941892414357311 98-99 0.02242600986565026 100-101 0.038483032929455846 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1114777.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.98644649295537 #Duplication Level Percentage of deduplicated Percentage of total 1 75.05020611520494 33.011918755717346 2 13.414415514144707 11.801049404943932 3 4.75216808024706 6.270929609619532 4 2.2633005749211623 3.982181985449794 5 1.2177811636979463 2.678293299856432 6 0.7271850421586309 1.9191771568432867 7 0.4900763620500072 1.5089702373742406 8 0.3320655195434538 1.1685105766042847 9 0.2361617977518033 0.9349126452440858 >10 1.3273097338818243 10.74688096684694 >50 0.1144206479564533 3.478894494783062 >100 0.062219869865998666 5.38462489090916 >500 0.005526106799233969 1.655152530407213 >1k 0.004707424310458566 5.036129048190345 >5k 0.0016373649775508054 4.905229304964232 >10k+ 8.186824887754027E-4 5.517145092246137 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22701 2.0363713998405064 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16408 1.471863879502358 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12075 1.0831762765109074 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10099 0.905921094532808 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9959 0.8933625290080438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8539 0.7659827929711504 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7269 0.652058662853647 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6362 0.5706970990610678 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5976 0.5360713398285039 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5691 0.5105056885816626 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5410 0.48529885349267166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5280 0.4736373283625335 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4669 0.41882816025088426 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4653 0.4173928956194827 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3568 0.3200640128025605 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3507 0.3145920663953419 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3438 0.3084024876724224 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3224 0.2892058232274257 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2935 0.26328135582273404 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2772 0.24865959739033008 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2736 0.24543025196967647 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2513 0.2254262511695164 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2473 0.22183808959101237 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2311 0.207306035198071 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2076 0.18622558592435975 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2013 0.18057423143821588 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1872 0.16792596187398917 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1828 0.1639789841376347 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1745 0.1565335488622388 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1534 0.13760599653563 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1519 0.13626043594369097 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1336 0.11984459672203499 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.970403946260104E-5 0.0 12-13 0.0 0.0 0.0 1.7940807892520208E-4 0.0 14-15 0.0 0.0 0.0 1.7940807892520208E-4 0.0 16-17 0.0 0.0 0.0 1.7940807892520208E-4 0.0 18-19 0.0 0.0 0.0 1.7940807892520208E-4 0.0 20-21 0.0 0.0 0.0 2.242600986565026E-4 0.0 22-23 0.0 0.0 0.0 2.691121183878031E-4 0.0 24-25 0.0 0.0 0.0 4.485201973130052E-4 0.0 26-27 0.0 0.0 0.0 9.867444340886113E-4 0.0 28-29 0.0 0.0 0.0 0.0017492287695207202 0.0 30-31 0.0 0.0 0.0 0.004171237835010949 0.0 32-33 0.0 0.0 0.0 0.0074902872951271866 0.0 34-35 0.0 0.0 0.0 0.01202034128798854 0.0 36-37 0.0 0.0 0.0 0.017088619517625497 0.0 38-39 0.0 0.0 0.0 0.025475947207378696 0.0 40-41 0.0 0.0 0.0 0.03812421677160544 0.0 42-43 0.0 0.0 0.0 0.05691721303902036 0.0 44-45 0.0 0.0 0.0 0.07508228103019707 0.0 46-47 0.0 0.0 0.0 0.10894555592732896 0.0 48-49 0.0 0.0 0.0 0.14994030196173763 0.0 50-51 0.0 0.0 0.0 0.17487802493234073 0.0 52-53 0.0 0.0 0.0 0.2069023670204893 0.0 54-55 0.0 0.0 0.0 0.2592895260666483 0.0 56-57 0.0 0.0 0.0 0.33921582522782584 0.0 58-59 0.0 0.0 0.0 0.42519714705272893 0.0 60-61 0.0 0.0 0.0 0.5268318237638558 0.0 62-63 0.0 0.0 0.0 0.6318752539745618 0.0 64-65 0.0 0.0 0.0 0.7406414018229655 0.0 66-67 0.0 0.0 0.0 0.8790995867334902 0.0 68-69 0.0 0.0 0.0 1.0518247147187285 0.0 70-71 0.0 0.0 0.0 1.1925255006158182 0.0 72-73 0.0 0.0 0.0 1.3497318297740266 0.0 74-75 0.0 0.0 0.0 1.5176577916480156 0.0 76-77 0.0 0.0 0.0 1.705856866440553 0.0 78-79 0.0 0.0 0.0 1.9393116291419719 0.0 80-81 0.0 0.0 0.0 2.2301769770994557 0.0 82-83 0.0 0.0 0.0 2.53140314161487 0.0 84-85 0.0 0.0 0.0 2.8859583575908005 0.0 86-87 0.0 0.0 0.0 3.2819119877787215 0.0 88-89 0.0 0.0 0.0 3.732540230019098 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9810 0.0 25.670263 1 TACATGG 9880 0.0 25.056692 2 ATGGGAG 2415 0.0 23.808426 5 ACATGGG 9860 0.0 23.80636 3 GAGTACT 7455 0.0 22.880749 12-13 CATGGGA 6630 0.0 22.505987 4 GTATAGG 275 5.0931703E-11 22.461262 1 AGTACTT 7595 0.0 21.833385 12-13 TATCGTA 110 0.0028700803 21.600275 7 GGTAAGG 335 1.8189894E-12 21.27597 3 GTACTTT 8050 0.0 21.159101 14-15 CATGGGG 2755 0.0 20.698627 4 CCCTATA 115 0.0037157983 20.659275 2 GCTTATG 325 2.1827873E-11 20.467657 1 TACTTTT 8475 0.0 19.537413 14-15 ACTTTTT 8980 0.0 19.28438 16-17 GTAAGGT 370 7.2759576E-12 19.26511 4 TAAGGTG 425 0.0 19.007387 5 ATGGGGG 1340 0.0 18.794617 5 AGGTAAG 380 1.0913936E-11 18.756447 2 >>END_MODULE