##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936381_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1114777 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72004086916038 31.0 31.0 34.0 30.0 34.0 2 31.828565713142627 31.0 31.0 34.0 30.0 34.0 3 31.909985584560857 31.0 31.0 34.0 30.0 34.0 4 35.67296867445238 37.0 35.0 37.0 33.0 37.0 5 35.473761119936995 37.0 35.0 37.0 33.0 37.0 6 35.640735321952285 37.0 35.0 37.0 33.0 37.0 7 35.580018245801625 37.0 35.0 37.0 33.0 37.0 8 35.573050933056564 37.0 35.0 37.0 33.0 37.0 9 37.13381689790873 39.0 37.0 39.0 33.0 39.0 10-11 36.97930482957578 39.0 37.0 39.0 33.0 39.0 12-13 36.88673026085038 39.0 37.0 39.0 33.0 39.0 14-15 37.753349324573435 39.0 37.0 41.0 32.0 41.0 16-17 37.75880333017276 39.0 37.0 41.0 32.5 41.0 18-19 38.04806163026327 40.0 37.0 41.0 33.0 41.0 20-21 38.10563951355293 40.0 37.0 41.0 33.0 41.0 22-23 38.0040555196241 40.0 37.0 41.0 33.0 41.0 24-25 37.80481163497274 39.5 37.0 41.0 32.5 41.0 26-27 37.500323383062266 39.0 36.5 41.0 32.0 41.0 28-29 37.414316495586114 39.0 36.0 41.0 32.0 41.0 30-31 37.344084511969655 39.0 36.0 41.0 32.0 41.0 32-33 37.08705732177826 39.0 35.0 41.0 31.5 41.0 34-35 36.78962429257152 39.0 35.0 40.0 31.0 41.0 36-37 36.435472744773165 38.5 35.0 40.0 30.5 41.0 38-39 36.2074020185203 38.0 35.0 40.0 30.0 41.0 40-41 36.0068448667312 38.0 35.0 40.0 30.0 41.0 42-43 35.63945659086974 38.0 35.0 40.0 29.0 41.0 44-45 35.224817160741566 38.0 34.5 40.0 27.0 41.0 46-47 34.63705117705155 38.0 34.0 40.0 24.5 41.0 48-49 34.05704145313368 38.0 33.0 40.0 23.0 41.0 50-51 33.63720950468121 38.0 33.0 40.0 20.5 41.0 52-53 33.39283372369542 37.5 33.0 40.0 19.5 41.0 54-55 33.13826352714489 37.0 32.5 40.0 18.5 41.0 56-57 32.723788255408934 36.5 31.5 40.0 15.5 41.0 58-59 32.34768792323487 36.0 31.0 39.0 13.0 40.5 60-61 31.82182176345583 35.5 31.0 39.0 8.0 40.0 62-63 31.491256995793776 35.0 31.0 39.0 8.0 40.0 64-65 31.080992880190387 35.0 30.0 38.0 7.0 40.0 66-67 30.5247210877153 35.0 29.0 38.0 2.0 40.0 68-69 30.18989762077976 34.0 29.0 37.0 2.0 39.0 70-71 29.643863301808345 34.0 28.5 36.5 2.0 39.0 72-73 29.13654434922859 34.0 28.0 36.0 2.0 39.0 74-75 28.63778450757416 33.5 27.0 35.5 2.0 38.0 76-77 27.33512711510912 31.5 26.0 34.0 2.0 36.0 78-79 28.12134444826185 33.0 27.0 35.0 2.0 37.0 80-81 27.839392542185568 33.0 26.0 35.0 2.0 36.0 82-83 27.440832112610863 33.0 26.0 35.0 2.0 36.0 84-85 26.98831918850138 33.0 25.0 35.0 2.0 35.5 86-87 26.679976354015196 32.5 25.0 35.0 2.0 35.0 88-89 26.20114516176778 32.0 24.5 34.0 2.0 35.0 90-91 25.57474947904379 32.0 22.0 34.0 2.0 35.0 92-93 25.032340100307056 31.0 19.5 34.0 2.0 35.0 94-95 24.330951392072137 31.0 15.5 34.0 2.0 35.0 96-97 23.409186770089445 31.0 2.0 34.0 2.0 35.0 98-99 22.42561068267465 30.5 2.0 34.0 2.0 35.0 100-101 19.296370036339106 26.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2911185870775981 1101 2 0.4486179406781474 1101 3 0.3323380040500439 1101 4 0.2638601018931013 1101 5 0.16923290059148854 1101 6 0.1665667887950093 1101 7 0.17265199153884936 1101 8 0.19924856973796778 1101 9 0.331558942488833 1101 10-11 0.3638349886526555 1101 12-13 0.2320649618294155 1101 14-15 0.41890876835780233 1101 16-17 0.34425744927041535 1101 18-19 0.42184676000292143 1101 20-21 0.3642411805701826 1101 22-23 0.24546450474407777 1101 24-25 0.3064781804955672 1101 26-27 0.24592894609045146 1101 28-29 0.26208861883582557 1101 30-31 0.3683442143845497 1101 32-33 0.544666807222633 1101 34-35 0.5830468713692554 1101 36-37 0.41097616359951417 1101 38-39 0.46533964494007307 1101 40-41 0.3724886060562014 1101 42-43 0.6080041463388355 1101 44-45 0.6475892416846847 1101 46-47 0.6754882836563993 1101 48-49 0.7710030627770408 1101 50-51 0.7863912216238944 1101 52-53 0.6284389750687112 1101 54-55 0.7013792839774524 1101 56-57 0.7441333901244889 1101 58-59 0.7892009874769954 1101 60-61 0.7675931514998062 1101 62-63 0.8821837931093945 1101 64-65 0.7083642209604655 1101 66-67 0.6985414576346436 1101 68-69 0.7202535998068953 1101 70-71 0.858868858792075 1101 72-73 0.9276901649369975 1101 74-75 0.9119267832105677 1101 76-77 0.6715434797178048 1101 78-79 0.5834208698245291 1101 80-81 0.9390875067265121 1101 82-83 1.0160722488878946 1101 84-85 1.1679840090189408 1101 86-87 1.2250244730543898 1101 88-89 0.9112141181736213 1101 90-91 0.9345165897507108 1101 92-93 0.9430265157286257 1101 94-95 1.08366480994059 1101 96-97 1.1427193329369807 1101 98-99 1.3159559647342043 1101 100-101 1.7206121733648203 1102 1 0.15485168248682157 1102 2 0.19427045581689484 1102 3 0.16579443006638428 1102 4 0.08794330924231275 1102 5 0.01003358131880816 1102 6 0.09593141575808772 1102 7 0.15489442629559846 1102 8 0.14305469463160847 1102 9 0.08782634275484469 1102 10-11 0.24676104216906225 1102 12-13 0.0965134818257809 1102 14-15 0.24225218042088414 1102 16-17 0.17252993846882703 1102 18-19 0.18168673951501546 1102 20-21 0.019665519876404858 1102 22-23 -0.06484512705183931 1102 24-25 0.055996549393604766 1102 26-27 1.867963469592837E-4 1102 28-29 -0.00408030728377895 1102 30-31 0.1275712088577734 1102 32-33 0.0969118029680871 1102 34-35 0.26361020627222587 1102 36-37 0.4259678610393536 1102 38-39 0.38356892778062246 1102 40-41 0.4034488942087364 1102 42-43 0.5089938445552278 1102 44-45 0.4711197711484516 1102 46-47 0.40344684520686513 1102 48-49 0.4126517612164875 1102 50-51 0.17642236655107268 1102 52-53 0.09130876944609412 1102 54-55 0.2517374093337139 1102 56-57 0.15434760443793039 1102 58-59 0.013463464035687878 1102 60-61 -0.03102597991195566 1102 62-63 0.12851331800468202 1102 64-65 0.27837091257611846 1102 66-67 0.31520771123861735 1102 68-69 0.2258704263288216 1102 70-71 0.0731930469173463 1102 72-73 0.31852306455449764 1102 74-75 0.17753394406544132 1102 76-77 -0.01716297314761306 1102 78-79 -0.3435124701178083 1102 80-81 0.0984912920330494 1102 82-83 0.05911709923528008 1102 84-85 -0.08639776004596555 1102 86-87 -0.04249558113943408 1102 88-89 0.030310006547559 1102 90-91 0.1679209698675237 1102 92-93 0.4252561455418409 1102 94-95 0.5111029124957973 1102 96-97 0.5029347864385159 1102 98-99 0.6873075884836588 1102 100-101 1.350984673302861 1103 1 0.0991729638786012 1103 2 0.09949944608842287 1103 3 0.16263305715628462 1103 4 0.2213593337418942 1103 5 0.08855045014762197 1103 6 0.10079311520529188 1103 7 0.01764738263161547 1103 8 0.10939397868506262 1103 9 0.14795668703080622 1103 10-11 0.08792213436886698 1103 12-13 0.05021699763771892 1103 14-15 0.1305738710409301 1103 16-17 0.2883592936637456 1103 18-19 0.058004260065210644 1103 20-21 0.1750978560117673 1103 22-23 0.07299504394470091 1103 24-25 0.06698441290075863 1103 26-27 0.20868648435268256 1103 28-29 0.27932717753503766 1103 30-31 0.10931573881175893 1103 32-33 0.14259529026931972 1103 34-35 0.09863245171786872 1103 36-37 0.1406795326691821 1103 38-39 0.10039142248348298 1103 40-41 0.26121712896372173 1103 42-43 0.2587710518936035 1103 44-45 0.31828738732615847 1103 46-47 0.33474595894019643 1103 48-49 0.3588965845634746 1103 50-51 0.5540250667812145 1103 52-53 0.34963538095149715 1103 54-55 0.17533812768151336 1103 56-57 0.2237807049170044 1103 58-59 0.401821088955991 1103 60-61 0.239213909021462 1103 62-63 0.3183973474488262 1103 64-65 0.2605669464439835 1103 66-67 0.3849443508657764 1103 68-69 0.6430465145462598 1103 70-71 0.4975251339645972 1103 72-73 0.5436315861412915 1103 74-75 0.6754391857384086 1103 76-77 0.27078280241605057 1103 78-79 0.38906615835873026 1103 80-81 0.7467524101055467 1103 82-83 0.7594487094132312 1103 84-85 0.6176198638259045 1103 86-87 0.4665831141326251 1103 88-89 0.41869341961276163 1103 90-91 0.2485123047126372 1103 92-93 0.518728024626725 1103 94-95 0.7509199773145134 1103 96-97 0.7771084243594295 1103 98-99 0.8784087338154976 1103 100-101 0.8560945308001102 1104 1 0.03138166082294447 1104 2 0.16630627952819665 1104 3 0.13545444270218 1104 4 0.07716416639975421 1104 5 0.05364379343474468 1104 6 0.16336133373101092 1104 7 0.15247637857874707 1104 8 -0.025467744464037878 1104 9 0.04612997844973421 1104 10-11 0.049270866587825424 1104 12-13 0.039586848631650184 1104 14-15 0.03165280728303088 1104 16-17 0.053668990291427576 1104 18-19 -0.0243484365677773 1104 20-21 0.0011324336274256552 1104 22-23 9.947573605586513E-4 1104 24-25 0.0739889186404028 1104 26-27 0.1357888532714 1104 28-29 0.18611656826067957 1104 30-31 -0.06648108010421083 1104 32-33 -0.0469708354845082 1104 34-35 -0.2597242181743411 1104 36-37 -0.07140290803708638 1104 38-39 0.022407807312923467 1104 40-41 -0.005852318158858338 1104 42-43 -0.059293125264936464 1104 44-45 0.17072529851691343 1104 46-47 0.03910433772306732 1104 48-49 -0.029962722079794446 1104 50-51 -0.005693231616568539 1104 52-53 0.009945599445380537 1104 54-55 0.09134376383640586 1104 56-57 0.01227718187793414 1104 58-59 -0.24786959475947867 1104 60-61 -0.016163081809615676 1104 62-63 -0.12850984351350903 1104 64-65 0.16497476983738935 1104 66-67 -0.003960508504551541 1104 68-69 0.33615469652365704 1104 70-71 0.21743733661142883 1104 72-73 0.0021662212506790013 1104 74-75 0.006527462500859116 1104 76-77 0.028817330498643656 1104 78-79 0.23219494962817322 1104 80-81 0.2029191879267671 1104 82-83 0.16673166172527232 1104 84-85 0.1805193347066556 1104 86-87 0.19809268728145923 1104 88-89 0.41455785115895694 1104 90-91 0.4641084970353653 1104 92-93 0.5361990433626076 1104 94-95 0.6230260523677167 1104 96-97 0.5001872049261955 1104 98-99 0.6735815599774462 1104 100-101 1.3186762824661002 1105 1 0.027973248339904444 1105 2 0.08015702579685069 1105 3 0.1084422310332549 1105 4 0.037147884174515866 1105 5 0.007060347030403591 1105 6 0.11406418312042632 1105 7 0.08365141250005337 1105 8 0.08704243192674 1105 9 0.015014646020965472 1105 10-11 0.008212521947392304 1105 12-13 -0.145544766022347 1105 14-15 0.14843164039022128 1105 16-17 0.14852380711639057 1105 18-19 0.020256719470211237 1105 20-21 0.2084241568296008 1105 22-23 0.1948020843619105 1105 24-25 0.10484644916957819 1105 26-27 0.2806898030678653 1105 28-29 0.19617084234480586 1105 30-31 0.19705759018073365 1105 32-33 0.14969402204602034 1105 34-35 0.20432191479716266 1105 36-37 -0.029313355798279872 1105 38-39 0.39394647759785784 1105 40-41 0.2487989708506433 1105 42-43 0.2197597919670855 1105 44-45 0.39736573270958786 1105 46-47 0.23179633229565155 1105 48-49 0.2872068844330897 1105 50-51 0.23819550651098353 1105 52-53 0.193044649648904 1105 54-55 0.4176178479612318 1105 56-57 0.09868695121308235 1105 58-59 0.1987979764752552 1105 60-61 0.1630662261958129 1105 62-63 0.4197506720902915 1105 64-65 0.3087359638672389 1105 66-67 0.47216160818473085 1105 68-69 0.590719146998552 1105 70-71 0.38619717378917784 1105 72-73 0.5755095048327661 1105 74-75 0.3436129441833522 1105 76-77 0.3073709261567146 1105 78-79 0.4289900649606011 1105 80-81 0.5780128107218836 1105 82-83 0.2745308476140096 1105 84-85 0.39508649888576386 1105 86-87 0.12552823680655933 1105 88-89 0.4112905512946412 1105 90-91 0.6891234224084961 1105 92-93 0.8218869673788873 1105 94-95 0.8466352789620153 1105 96-97 0.8760488060116778 1105 98-99 1.191117516558176 1105 100-101 1.0631240436601317 1106 1 0.05021553064776185 1106 2 0.14012835501408105 1106 3 0.11732336083241535 1106 4 -0.002093049754947174 1106 5 -0.06928796039987617 1106 6 -0.0507803275601475 1106 7 0.1293140340315233 1106 8 -0.01696438093253505 1106 9 -0.04608988878848663 1106 10-11 0.09863761597904386 1106 12-13 0.18634480888361793 1106 14-15 0.07513748099770368 1106 16-17 0.18580689561260044 1106 18-19 0.0528568188244094 1106 20-21 0.13335269277452966 1106 22-23 0.22058818896768884 1106 24-25 -0.03624048575343153 1106 26-27 0.12463586793196413 1106 28-29 0.002651574727174477 1106 30-31 0.1105907673860429 1106 32-33 0.32358828079274105 1106 34-35 0.11935732747192418 1106 36-37 0.17415978308468993 1106 38-39 0.2595534607786192 1106 40-41 0.4741552975840193 1106 42-43 0.5598622687253041 1106 44-45 0.538731710993595 1106 46-47 0.3828039257950522 1106 48-49 0.3075409984065445 1106 50-51 0.45084698617885266 1106 52-53 0.25092613428448374 1106 54-55 0.19213690267701367 1106 56-57 0.1274846234861613 1106 58-59 0.01954228135070224 1106 60-61 0.14242496581684705 1106 62-63 -0.12957329356154546 1106 64-65 0.11490046603174164 1106 66-67 0.2895673281478288 1106 68-69 0.34144996809003203 1106 70-71 0.40994509799611834 1106 72-73 0.023889889475448456 1106 74-75 0.15097395475747888 1106 76-77 -0.1768524788535757 1106 78-79 0.027529274385056368 1106 80-81 0.2218711600228538 1106 82-83 0.44773615596686156 1106 84-85 0.18271349484062682 1106 86-87 -0.011819006114450303 1106 88-89 0.12019297127902817 1106 90-91 0.45218864134256975 1106 92-93 0.6493203645827776 1106 94-95 0.8680035281991145 1106 96-97 0.5892927205670695 1106 98-99 1.051274212321431 1106 100-101 1.2611480306021505 1107 1 0.046416254250402744 1107 2 0.12241652481176857 1107 3 0.06571833573350716 1107 4 0.06772711299522882 1107 5 0.05388218772840503 1107 6 0.042183518893018856 1107 7 0.09508006434356275 1107 8 -0.02595503500261742 1107 9 0.07602168232831019 1107 10-11 0.16929189149425383 1107 12-13 0.15749435584649518 1107 14-15 0.23880484613184905 1107 16-17 0.2211520086362384 1107 18-19 0.2008207498670629 1107 20-21 0.1377286882115314 1107 22-23 0.006043570430023237 1107 24-25 0.10716195719096078 1107 26-27 0.21793354075433768 1107 28-29 -0.01348719311818769 1107 30-31 -0.04879999495858556 1107 32-33 0.03930899530805476 1107 34-35 -0.02895644152143717 1107 36-37 0.10775250403847281 1107 38-39 0.18479055302707081 1107 40-41 -0.05058380426896747 1107 42-43 -0.10637389787247997 1107 44-45 0.07084701613204913 1107 46-47 0.21441918622191025 1107 48-49 0.1486124571885128 1107 50-51 0.43054732187827227 1107 52-53 0.3654240473409942 1107 54-55 0.24011545561673842 1107 56-57 0.2006512067717665 1107 58-59 0.15734399392529497 1107 60-61 0.15995674125350945 1107 62-63 0.4138776070748058 1107 64-65 0.4337674865241503 1107 66-67 0.47126433827198255 1107 68-69 0.5593678945358462 1107 70-71 0.16978315746494488 1107 72-73 0.24955228558143006 1107 74-75 0.2725088068346331 1107 76-77 0.1339791445636962 1107 78-79 0.283606726936398 1107 80-81 0.5949249976062028 1107 82-83 0.5511036030319687 1107 84-85 0.5503823052465826 1107 86-87 0.6959602660775985 1107 88-89 0.5208323469602725 1107 90-91 0.5161966000048963 1107 92-93 0.6508236406127743 1107 94-95 0.7061342938789856 1107 96-97 0.7857898699013468 1107 98-99 0.9372388224968198 1107 100-101 1.0451246065647162 1108 1 -9.218565552018276E-4 1108 2 0.13759059223041703 1108 3 0.1303792939703179 1108 4 -0.039159808133177876 1108 5 -0.020226366256309802 1108 6 0.036078169134412974 1108 7 0.06729476914340893 1108 8 0.1770424319267363 1108 9 0.19681884182516285 1108 10-11 0.026674060408936384 1108 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2316 70-71 -1.1963235248767567 2316 72-73 -0.8091600041756202 2316 74-75 -0.7813294296304285 2316 76-77 -0.9072972075250334 2316 78-79 -0.7982557059099804 2316 80-81 -1.2663761316519349 2316 82-83 -1.2703163535962787 2316 84-85 -1.0431046715172094 2316 86-87 -1.4685661106009462 2316 88-89 -1.1612903866781288 2316 90-91 -1.815441011644591 2316 92-93 -1.659947168228321 2316 94-95 -1.6010885554486585 2316 96-97 -1.635102342386066 2316 98-99 -1.4824338518918303 2316 100-101 -1.6079341326567445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 20.0 5 61.0 6 130.0 7 322.0 8 682.0 9 1275.0 10 2119.0 11 3495.0 12 4888.0 13 6776.0 14 8528.0 15 12057.0 16 15659.0 17 19910.0 18 16711.0 19 10455.0 20 9332.0 21 9501.0 22 10237.0 23 11234.0 24 12993.0 25 15199.0 26 18313.0 27 21797.0 28 26804.0 29 32576.0 30 38935.0 31 47492.0 32 57643.0 33 72534.0 34 93628.0 35 123382.0 36 158404.0 37 170143.0 38 78253.0 39 3282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.950974374436896 19.220787611858327 15.216681204211222 18.611556809493553 2 16.034290502201916 24.203044555943197 35.99596666759366 23.766698274261223 3 23.609520058919518 25.783747076675212 30.41558532430403 20.19114754010124 4 12.456784652563858 17.233661593554164 38.540106174748786 31.769447579133196 5 11.822491735926405 41.1347683143981 32.57610348369388 14.466636465981619 6 28.859866537851204 37.96010488233927 15.813443633816032 17.366584945993488 7 26.874074366442795 35.85219286009668 19.626077681904093 17.647655091556427 8 27.172340297655946 29.970568104652322 18.802146079440103 24.054945518251632 9 25.89791433056739 18.737833594976454 21.472976003588247 33.89127607086791 10-11 26.28711470208442 26.01495105943331 25.13064348222347 22.5672907562588 12-13 30.312343296850376 24.55400994109083 25.32479881446078 19.808847947598018 14-15 23.974835850391184 25.983922107035546 28.91594203418582 21.125300008387452 16-17 20.216812791856043 29.05716621940263 28.66267978960126 22.06334119914007 18-19 19.96255753392831 28.756379078506285 31.32151093895909 19.959552448606313 20-21 22.785229691678246 24.256465642904367 30.702911882824996 22.25539278259239 22-23 21.08063158244117 25.381619556934233 31.601055119261577 21.936693741363015 24-25 23.37493238509503 25.31102856286818 31.319986472533774 19.99405257950302 26-27 22.15142748211442 23.91760299625468 34.11531992195385 19.81564959967705 28-29 19.85013149153973 28.391272891033143 31.920754312442558 19.83784130498457 30-31 20.909195700799856 25.355646546291577 31.45553930400609 22.279618448902482 32-33 21.065839353985652 26.05967666989004 31.821337777881435 21.05314619824287 34-35 23.061019891403568 24.80627804903212 31.599902213869036 20.53279984569528 36-37 22.4806810081986 24.150455550058023 33.314881959805014 20.05398148193836 38-39 21.278063585150335 25.941695295997903 32.75343518332864 20.02680593552311 40-41 20.92793358908425 26.765037925322705 31.477143214684105 20.82988527090894 42-43 20.497420100253684 27.30383432666052 29.507154826278377 22.691590746807417 44-45 22.0539108655987 25.08737259658081 29.872053866013136 22.986662671807355 46-47 23.65608711701404 27.067264155640164 29.45846095607505 19.818187771270743 48-49 23.075387748145356 26.893978130017853 28.938121765027763 21.092512356809028 50-51 20.246939716496556 27.766993252395572 31.215968194221926 20.770098836885946 52-53 22.360364061731893 28.87824779593023 27.53575670370915 21.225631438628724 54-55 20.545102243113895 27.04255233259624 28.845520724470617 23.566824699819243 56-57 23.1301215951344 26.29451050221347 28.201280090422642 22.374087812229483 58-59 25.090478183197185 25.89351270641562 27.471818215661237 21.544190894725958 60-61 21.92221124803429 27.074413067835735 30.885895058655088 20.117480625474887 62-63 21.428247835434245 27.009268284149634 30.036498101982072 21.52598577843405 64-65 20.69804328264881 29.725130150277256 28.522759589583675 21.05406697749026 66-67 22.10433351992192 26.84632994603284 27.456151682167874 23.593184851877368 68-69 21.280253691443747 27.435231533810573 28.341272404327466 22.943242370418215 70-71 23.570609700962343 27.39061479615636 27.45291430744831 21.585861195432983 72-73 22.739370648754402 25.91321773967116 29.947827662641615 21.399583948932825 74-75 20.85674929028849 30.378612979927127 27.5965079816473 21.168129748137076 76-77 22.029670662375672 27.07727155496249 27.46417878648561 23.42887899617623 78-79 22.102596949398553 27.757772951501302 28.10350658589382 22.036123513206324 80-81 23.21810311960349 27.316782166005066 27.72701787436363 21.738096840027808 82-83 22.377491579795816 26.683558657055368 29.300202425790296 21.638747337358524 84-85 21.254346961175298 28.258107113429393 28.491305629104467 21.996240296290846 86-87 21.44245683062462 28.638010580074045 27.081544794870897 22.837987794430433 88-89 21.03052459236997 28.541278848094738 26.065041379774375 24.363155179760916 90-91 22.20295851644445 26.614132821481967 26.933696019320923 24.249212642752656 92-93 23.20588069076318 28.18266059185462 27.35863189348088 21.252826823901316 94-95 22.04893084322413 27.754165257540432 27.364304507619146 22.83259939161629 96-97 19.614234153338426 28.093161859918126 29.485377918933015 22.80722606781043 98-99 21.373482227637485 29.107948370509813 25.930062377186648 23.588507024666058 100-101 20.38581505313838 27.65416051047293 26.82624776739859 25.133776668990098 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1180.0 1 861.5 2 737.5 3 918.5 4 808.5 5 783.5 6 1260.0 7 2575.5 8 3835.0 9 4313.5 10 3766.5 11 2894.5 12 2731.5 13 2809.0 14 2898.5 15 2973.0 16 3091.0 17 3261.0 18 3384.0 19 3571.0 20 3832.5 21 4129.0 22 4472.5 23 4985.5 24 5614.0 25 6360.0 26 7500.0 27 8916.5 28 10056.5 29 11355.0 30 13243.5 31 15192.0 32 17690.5 33 19347.5 34 19896.5 35 20365.0 36 21688.0 37 23380.0 38 24062.5 39 23903.0 40 23883.5 41 25235.0 42 26350.0 43 27231.5 44 28351.5 45 29843.0 46 32557.0 47 53088.5 48 97703.5 49 92749.5 50 49230.5 51 32414.0 52 27337.0 53 26451.5 54 26297.5 55 25196.5 56 23642.0 57 21990.0 58 21557.0 59 20170.5 60 17211.0 61 14916.5 62 13145.5 63 10471.5 64 7578.0 65 5926.0 66 4489.0 67 3391.0 68 2520.5 69 1807.0 70 1437.0 71 1173.0 72 1026.5 73 978.0 74 788.5 75 685.5 76 574.0 77 372.0 78 260.5 79 205.0 80 158.5 81 111.0 82 84.5 83 62.0 84 37.5 85 21.5 86 5.5 87 1.5 88 2.0 89 2.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03776540061375504 2 0.005920466604531669 3 0.003229345420653637 4 9.867444340886115E-4 5 0.0010764484735512124 6 3.5881615785040416E-4 7 0.0 8 0.0 9 0.002422009065490228 10-11 5.830762565069067E-4 12-13 8.970403946260104E-4 14-15 0.0014801166511329172 16-17 0.003274197440384938 18-19 0.0 20-21 0.0 22-23 0.0019286368484459225 24-25 7.176323157008083E-4 26-27 0.004664610052055254 28-29 0.005965318624262969 30-31 0.0010315964538199118 32-33 2.691121183878031E-4 34-35 0.008925551926528804 36-37 0.012154897347182442 38-39 0.008835847887066203 40-41 0.0018389328089833215 42-43 4.485201973130052E-4 44-45 9.867444340886115E-4 46-47 4.485201973130052E-5 48-49 6.279282762382073E-4 50-51 9.867444340886115E-4 52-53 0.0018837848287146218 54-55 0.0028705292628032333 56-57 0.0010764484735512124 58-59 4.933722170443058E-4 60-61 0.0035881615785040414 62-63 0.005516798426949964 64-65 0.009329220104110508 66-67 0.002242600986565026 68-69 0.0033190494601162386 70-71 8.970403946260104E-5 72-73 8.970403946260104E-4 74-75 0.005696206505875166 76-77 0.003543309558772741 78-79 0.004126385815279647 80-81 0.004664610052055254 82-83 0.004260941874473549 84-85 0.005516798426949964 86-87 0.0035881615785040414 88-89 0.009149812025185305 90-91 0.011706377149869435 92-93 8.970403946260104E-4 94-95 0.0017940807892520207 96-97 6.727802959695078E-4 98-99 0.0023771570457589274 100-101 0.004126385815279647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1114777.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.345436014139 #Duplication Level Percentage of deduplicated Percentage of total 1 74.21120867927378 31.425059886601048 2 13.761140546591044 11.654429929944895 3 4.958455326098708 6.299038582208387 4 2.2938003398167894 3.8852790207568857 5 1.258958506881973 2.6655573448813286 6 0.8079276815872106 2.0527229966821734 7 0.491108477336023 1.4557341822123653 8 0.3503584716172297 1.1868865793503147 9 0.2582022747007842 0.9840319111842478 >10 1.404243154669531 10.934481584028983 >50 0.11889039847214714 3.484273689992375 >100 0.07124456061898433 5.66426344518516 >500 0.006805450510933693 2.0915547308430065 >1k 0.004891417554733592 4.811701583162189 >5k 0.001914032956200101 5.658860048309236 >10k+ 8.506813138667116E-4 5.746124484657433 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 31141 2.7934734929048592 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11825 1.0607502666452573 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10534 0.9449423516990394 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10306 0.9244898307015662 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9691 0.8693218464320666 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7530 0.6754714171533859 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7129 0.6395000973288828 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6905 0.6194063924892602 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6550 0.5875614584800368 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6533 0.5860364898091726 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6391 0.5732985162054832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6370 0.5714147313767687 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5738 0.5147217784364048 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4921 0.44143357819545975 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4454 0.399541791766425 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3448 0.30929952806704836 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3195 0.28660440608301035 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3101 0.27817222637352584 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2942 0.2639092840989723 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2835 0.25431095187647396 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2584 0.23179523797136106 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2561 0.22973204506372127 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2496 0.2239012824986522 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2377 0.2132265018026027 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2319 0.2080236675137718 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2079 0.18649469804274757 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2018 0.1810227516355289 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1987 0.17824192641218828 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1468 0.13168552993109833 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1460 0.1309678976153975 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1393 0.12495772697140325 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1299 0.11652554726191876 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1296 0.11625643514353096 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1118 0.10028911611918796 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.970403946260104E-5 0.0 12-13 0.0 0.0 0.0 1.7940807892520208E-4 0.0 14-15 0.0 0.0 0.0 1.7940807892520208E-4 0.0 16-17 0.0 0.0 0.0 1.7940807892520208E-4 0.0 18-19 0.0 0.0 0.0 1.7940807892520208E-4 0.0 20-21 0.0 0.0 0.0 2.242600986565026E-4 0.0 22-23 0.0 0.0 0.0 2.691121183878031E-4 0.0 24-25 0.0 0.0 0.0 4.485201973130052E-4 0.0 26-27 0.0 0.0 0.0 9.867444340886113E-4 0.0 28-29 0.0 0.0 0.0 0.0017043767497894199 0.0 30-31 0.0 0.0 0.0 0.004126385815279647 0.0 32-33 0.0 0.0 0.0 0.007579991334589788 0.0 34-35 0.0 0.0 0.0 0.012199749366913742 0.0 36-37 0.0 0.0 0.0 0.017402583655744604 0.0 38-39 0.0 0.0 0.0 0.025745059325766498 0.0 40-41 0.0 0.0 0.0 0.038483032929455846 0.0 42-43 0.0 0.0 0.0 0.05754514131525856 0.0 44-45 0.0 0.0 0.0 0.07584476536562917 0.0 46-47 0.0 0.0 0.0 0.10984259632195498 0.0 48-49 0.0 0.0 0.0 0.15043367417878195 0.0 50-51 0.0 0.0 0.0 0.17478832089287813 0.0 52-53 0.0 0.0 0.0 0.2066781069218328 0.0 54-55 0.0 0.0 0.0 0.2586615977904101 0.0 56-57 0.0 0.0 0.0 0.33822908079373726 0.0 58-59 0.0 0.0 0.0 0.42434495867783417 0.0 60-61 0.0 0.0 0.0 0.5261590434678864 0.0 62-63 0.0 0.0 0.0 0.6302157292445036 0.0 64-65 0.0 0.0 0.0 0.7381296887180127 0.0 66-67 0.0 0.0 0.0 0.8755114251549861 0.0 68-69 0.0 0.0 0.0 1.048595369298075 0.0 70-71 0.0 0.0 0.0 1.1884888188400011 0.0 72-73 0.0 0.0 0.0 1.344842959623315 0.0 74-75 0.0 0.0 0.0 1.514428446227362 0.0 76-77 0.0 0.0 0.0 1.7034797093947938 0.0 78-79 0.0 0.0 0.0 1.9368447680567504 0.0 80-81 0.0 0.0 0.0 2.22618514734337 0.0 82-83 0.0 0.0 0.0 2.5251687108722196 0.0 84-85 0.0 0.0 0.0 2.8747902046777067 0.0 86-87 0.0 0.0 0.0 3.265810112695185 0.0 88-89 0.0 0.0 0.0 3.7096208479364035 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 60 0.003943299 31.679127 1 TAAGGTG 475 0.0 30.999678 5 GGTAAGG 425 0.0 29.057219 3 GTAAGGT 450 0.0 28.498425 4 AGGTAAG 435 0.0 27.301016 2 GTGTGCA 655 0.0 24.655127 9 GTGTAGG 370 0.0 24.40149 1 GTACATG 9265 0.0 23.54137 1 TACATGG 9230 0.0 23.211485 2 AAGGTAA 515 0.0 23.067327 1 GGCGACA 270 4.0017767E-11 22.869106 6 GAGTACT 9340 0.0 22.60448 12-13 ACATGGG 9180 0.0 22.299967 3 AGTACTT 9435 0.0 22.20068 12-13 GTAAGCG 205 0.0 22.014929 94-95 CATGGGT 810 0.0 21.696333 4 GTACTTT 9720 0.0 21.5986 14-15 ATGGGAG 2245 0.0 21.157911 5 TCCGTGA 135 3.7972626E-4 21.109943 8 TAGGACA 360 0.0 21.109943 4 >>END_MODULE