##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936380_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1160069 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72009940788005 31.0 31.0 34.0 30.0 34.0 2 31.74590390743999 33.0 31.0 34.0 30.0 34.0 3 31.700593671583327 33.0 31.0 34.0 30.0 34.0 4 35.37557248749859 37.0 35.0 37.0 33.0 37.0 5 35.43646283109022 37.0 35.0 37.0 33.0 37.0 6 35.54660714147176 37.0 35.0 37.0 33.0 37.0 7 35.440024688186654 37.0 35.0 37.0 33.0 37.0 8 35.43904974617889 37.0 35.0 37.0 33.0 37.0 9 36.950221926454375 39.0 37.0 39.0 33.0 39.0 10-11 36.845900545571 39.0 37.0 39.0 33.0 39.0 12-13 36.77747789140129 39.0 37.0 39.0 32.5 39.0 14-15 37.70420854276772 40.0 37.0 41.0 32.0 41.0 16-17 37.699219184376105 40.0 37.0 41.0 32.5 41.0 18-19 37.6903296269446 40.0 37.0 41.0 32.0 41.0 20-21 37.686370810701774 40.0 37.0 41.0 32.0 41.0 22-23 37.67201390606938 40.0 37.0 41.0 32.0 41.0 24-25 37.73038500296103 40.0 37.0 41.0 32.5 41.0 26-27 37.61335705031338 40.0 37.0 41.0 32.0 41.0 28-29 37.513359119155844 40.0 37.0 41.0 32.0 41.0 30-31 37.29765557048762 39.0 36.5 41.0 32.0 41.0 32-33 37.01844157545801 39.0 35.5 41.0 31.0 41.0 34-35 36.73740268897798 39.0 35.0 41.0 31.0 41.0 36-37 36.45877572799549 39.0 35.0 40.0 30.5 41.0 38-39 36.224761199549334 38.0 35.0 40.0 30.0 41.0 40-41 36.02656436815396 38.0 35.0 40.0 30.0 41.0 42-43 35.66477942260332 38.0 35.0 40.0 29.0 41.0 44-45 35.35609821484756 38.0 35.0 40.0 27.5 41.0 46-47 34.96390516426178 38.0 34.5 40.0 25.0 41.0 48-49 34.54561797617211 38.0 34.0 40.0 23.0 41.0 50-51 33.456860755696425 37.0 33.0 39.5 22.0 40.5 52-53 33.569020894446794 37.5 33.0 39.5 22.0 40.5 54-55 34.01032438587705 38.0 34.0 40.0 22.0 41.0 56-57 33.76612511841968 38.0 33.5 40.0 20.0 41.0 58-59 33.51916523930904 37.5 33.0 40.0 19.5 41.0 60-61 33.10733068464031 37.0 33.0 40.0 18.0 41.0 62-63 32.84010649366546 36.0 32.5 40.0 15.5 41.0 64-65 32.496172210446105 36.0 32.0 39.0 13.0 41.0 66-67 32.03131580966305 35.5 31.5 39.0 8.0 40.5 68-69 31.56945535136272 35.0 31.0 38.5 7.5 40.0 70-71 31.076474330406207 35.0 31.0 37.5 4.0 40.0 72-73 30.65707341546063 35.0 30.5 37.0 2.0 39.0 74-75 30.291637393982597 35.0 30.0 36.5 2.0 39.0 76-77 29.869821536477573 34.0 29.5 36.0 2.0 38.5 78-79 29.374451433492318 34.0 29.0 35.5 2.0 37.5 80-81 28.854783637869815 34.0 29.0 35.0 2.0 37.0 82-83 28.426257834663282 34.0 28.0 35.0 2.0 36.5 84-85 28.050968519975967 34.0 27.0 35.0 2.0 36.0 86-87 27.83465293874761 34.0 27.0 35.0 2.0 36.0 88-89 27.435441340127184 34.0 26.0 35.0 2.0 35.5 90-91 27.009361512116953 33.0 25.0 35.0 2.0 35.0 92-93 26.56337941967245 33.0 25.0 35.0 2.0 35.0 94-95 26.04523696435298 33.0 24.0 35.0 2.0 35.0 96-97 25.43181440069513 33.0 19.5 35.0 2.0 35.0 98-99 24.782063825513827 32.0 10.0 35.0 2.0 35.0 100-101 22.351412286682944 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3569584444940297 1101 2 0.35215887514468847 1101 3 0.38682071132327067 1101 4 0.507685396777255 1101 5 0.41963222131973765 1101 6 0.3663267676859192 1101 7 0.46279997089511227 1101 8 0.29148200035312044 1101 9 0.43721592453712077 1101 10-11 0.6576918884138792 1101 12-13 0.5943642449129953 1101 14-15 0.8137829088267168 1101 16-17 0.860938095737211 1101 18-19 0.8648334387081533 1101 20-21 0.9718430575367805 1101 22-23 0.7139896698161081 1101 24-25 0.6188124614613884 1101 26-27 0.6748785472248571 1101 28-29 0.5916038815588394 1101 30-31 0.5398856681989983 1101 32-33 0.44064076858590795 1101 34-35 0.5207520332485203 1101 36-37 0.6800089018476783 1101 38-39 0.5592289264272594 1101 40-41 0.469194927103338 1101 42-43 0.5720403581924884 1101 44-45 0.6202624528283636 1101 46-47 0.8386301301902961 1101 48-49 1.131721935759785 1101 50-51 0.9375623516726748 1101 52-53 1.077527302404178 1101 54-55 1.0136097343304513 1101 56-57 1.1725702246004488 1101 58-59 1.1904051723367957 1101 60-61 1.2222553820137918 1101 62-63 1.2749351173970709 1101 64-65 1.151737395648503 1101 66-67 1.2841216167857645 1101 68-69 1.2524264606050473 1101 70-71 1.276163556432266 1101 72-73 1.3990080428248355 1101 74-75 1.1253383884600652 1101 76-77 1.254781596234107 1101 78-79 1.3111190090684524 1101 80-81 1.2043725170821489 1101 82-83 1.3279037666719518 1101 84-85 1.4209342562073601 1101 86-87 1.3640037226680164 1101 88-89 1.6002377375309997 1101 90-91 1.4963701402150278 1101 92-93 1.2055930141241866 1101 94-95 1.2371454430568924 1101 96-97 1.2667304153969283 1101 98-99 1.0644267552057904 1101 100-101 1.2855515931599584 1102 1 0.1238118971881903 1102 2 0.2479858019450667 1102 3 0.28089367176791313 1102 4 0.1671197995744862 1102 5 0.1960242897472071 1102 6 0.18095871948600717 1102 7 0.28600244011527565 1102 8 0.19469494781385777 1102 9 0.28653426943068183 1102 10-11 0.3758129804287762 1102 12-13 0.21056166863254333 1102 14-15 0.31857499547721346 1102 16-17 0.19335458302095532 1102 18-19 0.17648136982510465 1102 20-21 0.24828068353192378 1102 22-23 0.023120545318668917 1102 24-25 0.14878079895184726 1102 26-27 -0.10212854836630214 1102 28-29 -0.12743120915767037 1102 30-31 -0.10949759622850763 1102 32-33 0.009913897593548882 1102 34-35 -0.1821585258567353 1102 36-37 -0.09055749261252544 1102 38-39 -0.19144304769603337 1102 40-41 0.05865575328983397 1102 42-43 -0.01492049646748228 1102 44-45 0.09298770652706168 1102 46-47 0.15796874820147622 1102 48-49 0.4961540037316752 1102 50-51 0.32010924749391023 1102 52-53 0.2813319287751739 1102 54-55 0.3031166875542226 1102 56-57 0.12035840468951164 1102 58-59 0.19805030166333637 1102 60-61 0.1809147366908448 1102 62-63 0.24659261560324097 1102 64-65 0.35284558348235606 1102 66-67 0.3757891376568203 1102 68-69 0.4697047376178425 1102 70-71 0.38581777449356025 1102 72-73 0.6178096510069757 1102 74-75 0.3284197884441191 1102 76-77 0.2958574381949042 1102 78-79 0.40799717674308056 1102 80-81 0.523779471444854 1102 82-83 0.3083310966568007 1102 84-85 0.2975472788380813 1102 86-87 0.17133222689917815 1102 88-89 0.40693982520653904 1102 90-91 0.5671438216081768 1102 92-93 0.4566607696167182 1102 94-95 0.38299799598909345 1102 96-97 0.3647930798312764 1102 98-99 0.4092007327231322 1102 100-101 1.162301288493726 1103 1 0.34081446641456736 1103 2 0.43859222093287187 1103 3 0.4724959718372368 1103 4 0.47153560643391046 1103 5 0.49048454830109733 1103 6 0.45289379554326814 1103 7 0.5789929380558192 1103 8 0.2891886470920255 1103 9 0.3487715354475682 1103 10-11 0.388883817670326 1103 12-13 0.3542421873715895 1103 14-15 0.6578307354551924 1103 16-17 0.6428382908632102 1103 18-19 0.7818816389821563 1103 20-21 0.5956841336359204 1103 22-23 0.29495326013454104 1103 24-25 0.4619544052484912 1103 26-27 0.6060052960935423 1103 28-29 0.5339092745093907 1103 30-31 0.5165975874915674 1103 32-33 0.6489734791766821 1103 34-35 0.6337478401152907 1103 36-37 0.6636527761367077 1103 38-39 0.4493729875390642 1103 40-41 0.7105288416630628 1103 42-43 0.8920930837290868 1103 44-45 0.8666356001692535 1103 46-47 1.0109625086517084 1103 48-49 0.8637005964481261 1103 50-51 0.7317384632681367 1103 52-53 1.0143089688632614 1103 54-55 0.9426880753442788 1103 56-57 0.9588005563146567 1103 58-59 0.8679540576276139 1103 60-61 0.8545503959217058 1103 62-63 0.9395745494558554 1103 64-65 1.024534340058345 1103 66-67 0.9861015229592134 1103 68-69 0.9931882823346108 1103 70-71 1.0392075220568735 1103 72-73 0.9987182599544084 1103 74-75 1.098262372351794 1103 76-77 0.8895298006597336 1103 78-79 0.9589290310720209 1103 80-81 1.2291834109253017 1103 82-83 1.213891228788441 1103 84-85 1.295560818253275 1103 86-87 1.288640248594863 1103 88-89 1.2902190552542514 1103 90-91 1.3713965029833268 1103 92-93 1.507464978254216 1103 94-95 1.3735750523897252 1103 96-97 1.4835843199927687 1103 98-99 1.6235574065945073 1103 100-101 1.9792365684578392 1104 1 0.18110681806474105 1104 2 0.08709360815387512 1104 3 0.32533377050385326 1104 4 0.2997471265796747 1104 5 0.31640943131903043 1104 6 0.26287433303964036 1104 7 0.24450786621019205 1104 8 0.19748629916871607 1104 9 0.27167600354148647 1104 10-11 0.24476025147710345 1104 12-13 0.36566667699847955 1104 14-15 0.3331833380474265 1104 16-17 0.46270619429606796 1104 18-19 0.2981295167410636 1104 20-21 0.37976753253442297 1104 22-23 0.27015554594347435 1104 24-25 0.3445056496064325 1104 26-27 0.08004769950569113 1104 28-29 -0.09618389705050845 1104 30-31 -0.0396415942550945 1104 32-33 0.0831852477799373 1104 34-35 0.16090383001135677 1104 36-37 0.22516422588618212 1104 38-39 0.11621012365235828 1104 40-41 0.14630175151440739 1104 42-43 0.29984412932103766 1104 44-45 0.3793263948191381 1104 46-47 0.3565279564963362 1104 48-49 0.5835403413651008 1104 50-51 0.5062574909711515 1104 52-53 0.5258510617035483 1104 54-55 0.6939233231806341 1104 56-57 0.8810702155137875 1104 58-59 0.8160786176508097 1104 60-61 0.7761338983441632 1104 62-63 0.7706404321010965 1104 64-65 0.4817532725193203 1104 66-67 0.7121903879225719 1104 68-69 0.5908854954697382 1104 70-71 0.3825703942254961 1104 72-73 0.6043669410592152 1104 74-75 0.5800674160389008 1104 76-77 0.7516623941418636 1104 78-79 0.5973157137503939 1104 80-81 0.7323206008710557 1104 82-83 0.8200215033339759 1104 84-85 0.7173584082155919 1104 86-87 0.5557966525867499 1104 88-89 0.43145016213238563 1104 90-91 0.5497778024160169 1104 92-93 0.7460926647008606 1104 94-95 0.7930081552097192 1104 96-97 0.7775625385525444 1104 98-99 0.7523904845687497 1104 100-101 1.2138495959123894 1105 1 0.1438574421422132 1105 2 0.20418852228834083 1105 3 0.25133804391877845 1105 4 0.22501254244316016 1105 5 0.26354118379547486 1105 6 0.24326471074763845 1105 7 0.40528903976854735 1105 8 0.259960949288093 1105 9 0.25957956318740116 1105 10-11 0.32896262401686016 1105 12-13 0.2753748972591481 1105 14-15 0.543132826450254 1105 16-17 0.5194821912519956 1105 18-19 0.38194573481067806 1105 20-21 0.2547615740057907 1105 22-23 0.2564779002522286 1105 24-25 0.014664292818103775 1105 26-27 -0.0029418511167094152 1105 28-29 0.19964847803991148 1105 30-31 0.10619935275372683 1105 32-33 0.2557707892533827 1105 34-35 -0.02448794847354918 1105 36-37 -0.007155251603116142 1105 38-39 -0.08869573159511646 1105 40-41 -0.11520510811102724 1105 42-43 -0.1030324810497305 1105 44-45 0.06361574096992229 1105 46-47 0.2936282646672552 1105 48-49 0.28534922521770056 1105 50-51 0.3262409325336719 1105 52-53 0.5414067938081004 1105 54-55 0.34029656541468256 1105 56-57 0.4025349263892579 1105 58-59 0.5271828061828288 1105 60-61 0.20541306272846782 1105 62-63 0.37235063435655746 1105 64-65 0.2994360948129966 1105 66-67 0.30053989639622003 1105 68-69 0.3082791723209546 1105 70-71 0.4728483752669206 1105 72-73 0.48849760218410054 1105 74-75 0.4207148258560487 1105 76-77 0.43219765817785927 1105 78-79 0.5863641471800385 1105 80-81 0.6776104362484077 1105 82-83 0.5903354864326573 1105 84-85 0.5193466751409481 1105 86-87 0.5844509032129146 1105 88-89 0.722379399900678 1105 90-91 0.5834265544701367 1105 92-93 0.545923526115935 1105 94-95 0.3832125431956541 1105 96-97 0.34496290568153753 1105 98-99 0.585989895824305 1105 100-101 1.1068093329844721 1106 1 -0.03230352565029193 1106 2 -0.15145780494258076 1106 3 -0.14523043837493788 1106 4 0.0331088339653931 1106 5 0.08200257486656426 1106 6 0.04887620023534822 1106 7 0.11249043676204451 1106 8 0.15676066710237535 1106 9 0.25553969139029675 1106 10-11 0.27101983630065263 1106 12-13 0.30409469729050187 1106 14-15 0.03769661435807592 1106 16-17 -0.14878323751353406 1106 18-19 0.09221198226315863 1106 20-21 -0.10380655120106042 1106 22-23 -0.16708874435053644 1106 24-25 -0.5007629485552059 1106 26-27 -0.2811216661282927 1106 28-29 -0.3110941755509842 1106 30-31 -0.2685365336055483 1106 32-33 -0.12016961207741161 1106 34-35 -0.2156578869849355 1106 36-37 -0.453917518843447 1106 38-39 -0.37694234797930193 1106 40-41 -0.45311140329122424 1106 42-43 -0.047989717719943314 1106 44-45 -0.16918508294270396 1106 46-47 -0.16844192467587504 1106 48-49 -0.057803198618287865 1106 50-51 -0.2632343675063922 1106 52-53 -0.1924139050370286 1106 54-55 0.07095312623267347 1106 56-57 -0.025439999145064007 1106 58-59 -0.07168627357818025 1106 60-61 0.13808455151942667 1106 62-63 0.05870599675618138 1106 64-65 -0.1700464776059576 1106 66-67 -0.19632731954552796 1106 68-69 -0.09391582323723213 1106 70-71 -0.18345333700816013 1106 72-73 -0.05955670985756356 1106 74-75 0.15840425608475428 1106 76-77 -0.019639597304731637 1106 78-79 -0.0224169237321874 1106 80-81 0.3952982116843131 1106 82-83 0.18389856016674244 1106 84-85 0.4346254206338145 1106 86-87 0.44134725221742954 1106 88-89 0.3962471732463193 1106 90-91 0.47211713434437286 1106 92-93 0.5059422078557319 1106 94-95 0.605631719631404 1106 96-97 0.4594386411933904 1106 98-99 0.5580506789767732 1106 100-101 1.0955915796705291 1107 1 0.13071303532179357 1107 2 0.19773441584218077 1107 3 0.2003467809469015 1107 4 0.2594667949001135 1107 5 0.2759903215802808 1107 6 0.22025212161126717 1107 7 0.30371405571163734 1107 8 0.34132975751795414 1107 9 0.42167773826722055 1107 10-11 0.38673887965599363 1107 12-13 0.2759726826189919 1107 14-15 0.20652233815150822 1107 16-17 0.3466790631854906 1107 18-19 0.5465595899464972 1107 20-21 0.08461262152585647 1107 22-23 0.29717389934180716 1107 24-25 0.29946349577095077 1107 26-27 0.20085400002957954 1107 28-29 0.1424796702395028 1107 30-31 0.08890158306856932 1107 32-33 0.20504105737644096 1107 34-35 0.17101728107474656 1107 36-37 0.21551915077255757 1107 38-39 0.241622121015574 1107 40-41 0.3001112092391409 1107 42-43 0.2038010387336513 1107 44-45 0.5297878318659386 1107 46-47 0.7148268844358299 1107 48-49 0.5167707476378638 1107 50-51 0.5400672956410304 1107 52-53 0.4464179700753306 1107 54-55 0.35575588745221864 1107 56-57 0.6641340881155315 1107 58-59 0.6978917529941029 1107 60-61 0.694341579709743 1107 62-63 0.9193931642941422 1107 64-65 0.7097309292517906 1107 66-67 0.4837009870968352 1107 68-69 0.705819749364192 1107 70-71 0.8685451366187209 1107 72-73 0.660936347436067 1107 74-75 0.7885207345692429 1107 76-77 0.7476638513114828 1107 78-79 0.91327866000859 1107 80-81 0.7158927481145767 1107 82-83 0.7814932446516707 1107 84-85 0.7177920896505583 1107 86-87 0.9841264586444112 1107 88-89 0.8428924131125051 1107 90-91 0.8153816604460609 1107 92-93 1.0831238550696654 1107 94-95 1.1908761772754346 1107 96-97 1.3943789698747509 1107 98-99 1.4138240619201703 1107 100-101 1.8450989453902373 1108 1 0.013741867993104506 1108 2 0.09472599353840394 1108 3 0.24000847450368568 1108 4 0.313120778627038 1108 5 0.1250829223619192 1108 6 0.23875997553425776 1108 7 0.4543734694487682 1108 8 0.23930407455285518 1108 9 -0.003661921924859257 1108 10-11 0.10259740587633814 1108 12-13 -0.04588437990580019 1108 14-15 0.48826992864805874 1108 16-17 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-2.088181126105937 2316 56-57 -2.9308043521170646 2316 58-59 -3.1328426328994823 2316 60-61 -3.748474611050497 2316 62-63 -3.1867497045182986 2316 64-65 -3.510969053081002 2316 66-67 -2.9661611896930147 2316 68-69 -3.161295309744961 2316 70-71 -2.95884862742718 2316 72-73 -1.9411506626076438 2316 74-75 -2.2991318825519933 2316 76-77 -2.2908392613464024 2316 78-79 -2.6929302233669468 2316 80-81 -3.655526080568613 2316 82-83 -3.4360418380517537 2316 84-85 -3.2133570956632447 2316 86-87 -3.7546961834958665 2316 88-89 -4.987983975330483 2316 90-91 -4.342916037687864 2316 92-93 -3.4978190228613357 2316 94-95 -3.25108609848877 2316 96-97 -4.6719385953181884 2316 98-99 -6.2247813495642745 2316 100-101 -7.439206305949526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2131.0 3 819.0 4 785.0 5 816.0 6 917.0 7 1057.0 8 1408.0 9 1768.0 10 2349.0 11 3246.0 12 4699.0 13 6156.0 14 7350.0 15 9939.0 16 13697.0 17 18903.0 18 14048.0 19 8578.0 20 8146.0 21 8361.0 22 9057.0 23 10136.0 24 11416.0 25 13133.0 26 15205.0 27 17877.0 28 21368.0 29 25694.0 30 31843.0 31 39781.0 32 50752.0 33 65635.0 34 88794.0 35 123627.0 36 163570.0 37 194256.0 38 140882.0 39 21869.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.03504449574366 18.03224949362153 14.459391379661357 19.473314630973455 2 15.361752248772142 22.14695656972573 37.81170672148336 24.679584460018763 3 22.602888534834157 26.01328050101043 30.340133943043167 21.043697021112237 4 11.817851837354409 15.96124316396904 39.40347304428946 32.81743195438709 5 11.904930245391368 40.06130473020745 33.182758799082585 14.851006225318594 6 28.74714484157266 36.456697733894494 17.144401820058413 17.651755604474438 7 26.380454896412502 35.633839876117726 20.3116898802155 17.674015347254272 8 26.633282417465836 29.391277948024413 19.78327262816438 24.192167006345365 9 26.146157077583034 18.158143755601504 22.439137159305677 33.256562007509785 10-11 25.624843398320063 25.500467702642034 25.63674620414778 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22.01173934144863 24.17047475455397 32.96799826901178 20.849787634985617 38-39 21.41373431812255 25.739163044110768 32.00786222146551 20.839240416301177 40-41 20.998025482950663 26.685419047171603 31.470163931034573 20.84639153884316 42-43 20.401723544170235 26.99772380795163 29.277320877564488 23.323231770313647 44-45 21.305909019792416 25.285080885518962 29.834177466703387 23.574832627985227 46-47 23.16082424727246 26.812086741728585 29.409140612440083 20.617948398558873 48-49 22.477926835838584 26.499046387586876 29.067399385808955 21.955627390765585 50-51 20.058669404457273 27.395232744527327 31.193615499894616 21.352482351120784 52-53 21.8617693696536 28.49810162072326 27.676296701444787 21.96383230817835 54-55 20.292069463259217 26.54847803983651 29.244918287011995 23.914534209892278 56-57 22.58058120359539 26.203944515754184 27.997329404736554 23.218144875913868 58-59 24.285088690983663 25.89287946353528 27.681396918551876 22.14063492692918 60-61 22.074207979407316 26.523773009770924 30.729980901248982 20.672038109572778 62-63 21.324936554349474 27.08698260761624 29.914193205798377 21.673887632235907 64-65 20.567614011782943 29.220566234780502 28.646676720687996 21.565143032748555 66-67 21.56725835815955 27.057558893302435 27.49236249034536 23.882820258192652 68-69 20.897555256715286 27.124797420778595 28.693191614082274 23.28445570842385 70-71 22.942204314978984 26.97870440290608 27.60346087933989 22.47563040277505 72-73 22.10470636771254 25.908366085596896 30.021550321228684 21.965377225461882 74-75 20.549256711494742 29.788397922947667 28.022791364524753 21.639554001032838 76-77 21.331127763536706 27.05914255240815 27.859847162118 23.749882521937142 78-79 21.618484002769307 27.295649377466358 28.694658048362857 22.391208571401474 80-81 22.887620824381784 27.17871835634011 27.689948170090382 22.24371264918772 82-83 21.850839576403672 26.542829667949547 29.491271482936277 22.115059272710504 84-85 21.42790026482028 27.804146782157733 28.463697553170945 22.30425539985104 86-87 21.392104608697938 28.45486405188023 27.767742347032236 22.385288992389597 88-89 20.988805286639057 28.144534339491223 26.42164411819597 24.445016255673753 90-91 21.404381272330177 26.599744570176608 27.40905107777506 24.586823079718158 92-93 22.811072422137602 27.927744247014775 27.762480354976837 21.49870297587078 94-95 21.73387152394615 27.2359854336791 27.93964494592805 23.0904980964467 96-97 19.55184159276243 27.52216265053489 29.863347204656765 23.06264855204591 98-99 21.160728989642152 28.717439344988822 26.56775280501674 23.554078860352288 100-101 20.62324274876556 27.006467288075825 27.752606616274527 24.617683346884085 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 636.0 1 488.0 2 580.0 3 850.5 4 760.0 5 750.5 6 1274.0 7 2396.5 8 3826.0 9 4496.5 10 3776.5 11 2875.0 12 2715.5 13 2831.0 14 2958.0 15 3009.5 16 2968.5 17 3072.5 18 3244.5 19 3386.5 20 3548.5 21 3853.0 22 4325.5 23 4901.0 24 5646.0 25 6591.5 26 7716.5 27 8948.0 28 10206.0 29 11741.0 30 13615.0 31 15682.5 32 18484.0 33 19991.5 34 20680.5 35 21443.0 36 22654.5 37 24353.5 38 24923.0 39 24826.0 40 24974.0 41 26536.5 42 27583.0 43 28542.5 44 29901.5 45 32080.0 46 35624.5 47 55408.0 48 98766.5 49 95002.0 50 52344.5 51 34592.5 52 29328.5 53 28375.0 54 28044.5 55 26565.0 56 24668.5 57 23039.0 58 22502.0 59 20863.0 60 18155.0 61 16269.0 62 14404.5 63 11012.5 64 7746.5 65 6184.0 66 4714.0 67 3629.5 68 2745.5 69 1911.5 70 1470.5 71 1213.5 72 1035.5 73 1015.5 74 837.5 75 675.0 76 532.0 77 325.0 78 224.0 79 155.5 80 105.5 81 84.5 82 62.0 83 35.5 84 20.0 85 15.0 86 10.0 87 7.5 88 5.0 89 3.0 90 4.0 91 2.0 92 2.0 93 3.0 94 1.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0738749160610274 2 0.028015574935628833 3 0.014912906042657806 4 0.01913679272526031 5 0.024912311250451485 6 0.028101776704661536 7 0.0779263992055645 8 0.05516913218093062 9 0.0674097833835746 10-11 0.05848790028868973 12-13 0.03823048456600426 14-15 0.033273882846623776 16-17 0.042669875671188524 18-19 0.03745466864470993 20-21 0.017024849383959057 22-23 0.011680339703931404 24-25 0.020128113069136404 26-27 0.022455560833019413 28-29 0.00568931675615847 30-31 7.327150367779848E-4 32-33 0.008749479556819465 34-35 0.009180488401982986 36-37 0.0032756672232427558 38-39 0.007887461866492425 40-41 0.0033187681077591073 42-43 0.0015085309580723217 44-45 3.017061916144643E-4 46-47 3.8790796064716836E-4 48-49 5.603114987125766E-4 50-51 8.189168058106889E-4 52-53 4.3100884516352046E-5 54-55 2.1550442258176024E-4 56-57 0.0024136495329157145 58-59 0.002586053070981123 60-61 0.003922180490988036 62-63 0.0018102371496867859 64-65 2.1550442258176024E-4 66-67 4.3100884516352047E-4 68-69 0.0024998513019484185 70-71 0.0014654300735559697 72-73 0.019438498916874773 74-75 0.013447475969101839 76-77 0.023964091791091737 78-79 0.018274775034933268 80-81 0.010430414052957195 82-83 0.004266987567118853 84-85 0.003189465454210052 86-87 0.001077522112908801 88-89 0.001077522112908801 90-91 0.00547381233357671 92-93 0.009137387517466634 94-95 0.011464835281349645 96-97 0.0105166158219899 98-99 0.023446881176895515 100-101 0.019998810415587348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1160069.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.616936659156444 #Duplication Level Percentage of deduplicated Percentage of total 1 74.39557814108336 32.44907219500956 2 13.690109454261371 11.942412738468738 3 4.953214156810376 6.4813208441050545 4 2.3073506269864064 4.02558264590924 5 1.2418131579409035 2.708204292620771 6 0.773420056118675 2.024052815918968 7 0.48955361122254265 1.4946980196368458 8 0.3544249612684184 1.2367144868854862 9 0.2561529118855185 1.0055344799492227 >10 1.3490416236137872 10.848026449434824 >50 0.11043868597309808 3.305310726258242 >100 0.06601650805003154 5.488312233267291 >500 0.005947432977936073 1.7998647759210462 >1k 0.0045596986164176566 4.917007476241863 >5k 0.0013877343615184173 3.9373933495827447 >10k+ 9.912388296560123E-4 6.33649247079002 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23539 2.0291034412608213 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 16880 1.455085861272045 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12104 1.0433862123718503 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10504 0.9054633819195237 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10253 0.883826737892315 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8638 0.7446108809044979 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7296 0.628928106862609 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6769 0.583499774582374 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6037 0.5204000796504347 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5921 0.5104006744426409 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5680 0.48962604810575927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5194 0.44773198835586503 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4892 0.4216990541079884 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4601 0.3966143393194715 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3658 0.3153260711216316 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3536 0.30480945529964165 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3470 0.2991201385434832 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3276 0.2823969953511386 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3052 0.2630877990878129 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2797 0.24110634798447336 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2797 0.24110634798447336 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2564 0.22102133579985328 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2478 0.21360798366304073 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2303 0.1985226740823175 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2061 0.17766184597640314 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2049 0.17662742474801066 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1991 0.17162772214411384 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1830 0.15774923732984847 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1814 0.15637000902532522 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1545 0.1331817331555278 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1532 0.13206111015810265 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1359 0.11714820411544485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2930265354905613E-4 0.0 24-25 0.0 0.0 0.0 3.4480707613081637E-4 0.0 26-27 0.0 0.0 0.0 9.48219459359745E-4 0.0 28-29 0.0 0.0 0.0 0.001853338034203138 0.0 30-31 0.0 0.0 0.0 0.004137684913569796 0.0 32-33 0.0 0.0 0.0 0.006508233561969159 0.0 34-35 0.0 0.0 0.0 0.009568396362630155 0.0 36-37 0.0 0.0 0.0 0.014137090121363471 0.0 38-39 0.0 0.0 0.0 0.02361928471496092 0.0 40-41 0.0 0.0 0.0 0.03581683503308855 0.0 42-43 0.0 0.0 0.0 0.052539978225433145 0.0 44-45 0.0 0.0 0.0 0.07029754264617019 0.0 46-47 0.0 0.0 0.0 0.1030973157631141 0.0 48-49 0.0 0.0 0.0 0.13844004106652277 0.0 50-51 0.0 0.0 0.0 0.1645591770834321 0.0 52-53 0.0 0.0 0.0 0.20020360857845526 0.0 54-55 0.0 0.0 0.0 0.2505023408090381 0.0 56-57 0.0 0.0 0.0 0.3258857878281378 0.0 58-59 0.0 0.0 0.0 0.4102342188266388 0.0 60-61 0.0 0.0 0.0 0.5075560160645617 0.0 62-63 0.0 0.0 0.0 0.607506967257982 0.0 64-65 0.0 0.0 0.0 0.7133627396301427 0.0 66-67 0.0 0.0 0.0 0.8487857187805208 0.0 68-69 0.0 0.0 0.0 1.0174825807775227 0.0 70-71 0.0 0.0 0.0 1.1638100837105378 0.0 72-73 0.0 0.0 0.0 1.3179388467410127 0.0 74-75 0.0 0.0 0.0 1.4830152344386411 0.0 76-77 0.0 0.0 0.0 1.6634355370240907 0.0 78-79 0.0 0.0 0.0 1.8951458921839994 0.0 80-81 0.0 0.0 0.0 2.1707760486660708 0.0 82-83 0.0 0.0 0.0 2.448130240528796 0.0 84-85 0.0 0.0 0.0 2.7904805662421803 0.0 86-87 0.0 0.0 0.0 3.1636911252692728 0.0 88-89 0.0 0.0 0.0 3.5897002678288965 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9880 0.0 26.599382 1 TACATGG 9915 0.0 26.072989 2 ATGGGAG 2375 0.0 25.812464 5 ACATGGG 9960 0.0 24.906605 3 CATGGGA 6810 0.0 23.726582 4 TAGCGCG 185 2.5298687E-7 23.119272 4 GAGTACT 7680 0.0 22.584803 12-13 GTGTAGG 295 5.456968E-12 22.553263 1 GTAAGCG 150 7.2759576E-12 22.167814 94-95 AGTACTT 7760 0.0 21.219059 12-13 GTACTTT 8180 0.0 21.202026 14-15 AGCGCGC 210 8.568004E-7 20.366978 5 GTATAGG 280 1.5661499E-9 20.366978 1 AACGCGG 70 4.858446E-4 20.354248 86-87 ATGGGAT 2295 0.0 20.293022 5 CATGGGT 800 0.0 20.197252 4 TAAGGTA 260 1.4819307E-8 20.105862 4 GTGTAAG 430 0.0 19.893326 1 CGTAACG 60 0.0047694524 19.788425 56-57 TGTAGCG 265 1.8453647E-8 19.725655 2 >>END_MODULE