##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936380_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1160069 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.711889551397373 31.0 31.0 34.0 30.0 34.0 2 31.84003365317063 31.0 31.0 34.0 30.0 34.0 3 31.89056685421298 31.0 31.0 34.0 30.0 34.0 4 35.622985356905495 37.0 35.0 37.0 33.0 37.0 5 35.480948978034924 37.0 35.0 37.0 33.0 37.0 6 35.62407580928376 37.0 35.0 37.0 33.0 37.0 7 35.56296392714571 37.0 35.0 37.0 33.0 37.0 8 35.551424958343 37.0 35.0 37.0 33.0 37.0 9 37.09495728271336 39.0 37.0 39.0 33.0 39.0 10-11 36.971490919936656 39.0 37.0 39.0 33.0 39.0 12-13 36.87633494214568 39.0 37.0 39.0 33.0 39.0 14-15 37.7614318631047 39.0 37.0 41.0 32.5 41.0 16-17 37.7343757138584 39.0 37.0 41.0 32.5 41.0 18-19 38.013099651831055 40.0 37.0 41.0 33.0 41.0 20-21 38.056718608979295 40.0 37.0 41.0 33.0 41.0 22-23 37.961342385668445 40.0 37.0 41.0 33.0 41.0 24-25 37.754506412980604 39.5 37.0 41.0 32.5 41.0 26-27 37.46470382365187 39.0 36.0 41.0 32.0 41.0 28-29 37.3802364342121 39.0 36.0 41.0 32.0 41.0 30-31 37.308279507512054 39.0 36.0 41.0 32.0 41.0 32-33 37.03373807937287 39.0 35.0 41.0 31.0 41.0 34-35 36.727498967733816 39.0 35.0 40.0 31.0 41.0 36-37 36.418235898037096 38.5 35.0 40.0 30.0 41.0 38-39 36.18159954278582 38.0 35.0 40.0 30.0 41.0 40-41 36.00978433179405 38.0 35.0 40.0 30.0 41.0 42-43 35.623203878389994 38.0 35.0 40.0 29.0 41.0 44-45 35.17474865719194 38.0 34.5 40.0 26.5 41.0 46-47 34.58609056875065 38.0 34.0 40.0 24.5 41.0 48-49 33.9838117387845 38.0 33.0 40.0 22.5 41.0 50-51 33.592968607901774 38.0 33.0 40.0 20.0 41.0 52-53 33.263322698908425 37.0 33.0 40.0 18.5 41.0 54-55 33.089992060817075 37.0 32.5 40.0 18.0 41.0 56-57 32.6877681413778 36.5 31.5 39.5 15.5 41.0 58-59 32.292421399071955 36.0 31.0 39.0 12.5 40.5 60-61 31.71992915938621 35.5 31.0 39.0 8.0 40.0 62-63 31.43637921537426 35.0 30.5 39.0 8.0 40.0 64-65 31.014528446152774 35.0 30.0 38.0 6.5 40.0 66-67 30.51258330323455 35.0 29.0 38.0 2.0 40.0 68-69 30.10022981391624 34.0 29.0 37.0 2.0 39.0 70-71 29.626452392055988 34.0 28.5 36.5 2.0 39.0 72-73 29.121161327472763 34.0 28.0 36.0 2.0 39.0 74-75 28.638484003968728 33.5 27.0 35.5 2.0 38.0 76-77 27.246757735962255 31.5 26.0 34.0 2.0 36.0 78-79 28.01471852105349 33.0 27.0 35.0 2.0 37.0 80-81 27.709835363241325 33.0 26.0 35.0 2.0 36.0 82-83 27.3570891041826 33.0 26.0 35.0 2.0 36.0 84-85 26.962369910755307 33.0 25.0 35.0 2.0 35.5 86-87 26.650700518676047 32.5 25.0 35.0 2.0 35.0 88-89 26.109868464720634 32.0 24.5 34.0 2.0 35.0 90-91 25.507639631780524 32.0 21.5 34.0 2.0 35.0 92-93 24.828661053782145 31.0 19.0 34.0 2.0 35.0 94-95 24.185531636480242 31.0 14.0 34.0 2.0 35.0 96-97 23.343731709062133 31.0 2.0 34.0 2.0 35.0 98-99 22.453704477923296 31.0 2.0 34.0 2.0 35.0 100-101 19.34183483913457 26.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18488922632525373 1101 2 0.24414854039145695 1101 3 0.20754038938943253 1101 4 0.2588016771797683 1101 5 0.14110742418847622 1101 6 0.10791258066311116 1101 7 0.21109691051601231 1101 8 0.10906741686505228 1101 9 0.14843022566990527 1101 10-11 0.2551106175026874 1101 12-13 0.3020135976297169 1101 14-15 0.4390369678426467 1101 16-17 0.34514874523200234 1101 18-19 0.29780620888342213 1101 20-21 0.3019983362201728 1101 22-23 0.30341375138328885 1101 24-25 0.2627594647269902 1101 26-27 0.2648725847707354 1101 28-29 0.21667731395834977 1101 30-31 0.28922634372701594 1101 32-33 0.24447053692247778 1101 34-35 0.3234165851293014 1101 36-37 0.4399522434061325 1101 38-39 0.3189703342172052 1101 40-41 0.33254155510933714 1101 42-43 0.545150178398849 1101 44-45 0.45353615792208046 1101 46-47 0.23102834639891512 1101 48-49 0.2596666288536724 1101 50-51 0.09697098711113483 1101 52-53 0.17828753185932555 1101 54-55 0.1377847438103501 1101 56-57 0.02750408732077858 1101 58-59 0.11986983763521408 1101 60-61 0.4112783445612571 1101 62-63 0.1902956719662967 1101 64-65 0.2874136126945359 1101 66-67 0.11075366849180668 1101 68-69 0.27551214606886987 1101 70-71 0.21157690275446583 1101 72-73 0.36177030548537914 1101 74-75 0.38382461870796547 1101 76-77 0.015625052582567633 1101 78-79 0.23243722660458843 1101 80-81 0.2162736190076302 1101 82-83 0.27942581751714357 1101 84-85 0.19598729261903003 1101 86-87 0.09901537748399747 1101 88-89 0.09733211330540215 1101 90-91 0.2806736391326865 1101 92-93 0.25116097392701064 1101 94-95 0.12607849186568032 1101 96-97 0.19888428693571214 1101 98-99 0.26115032302258 1101 100-101 0.976345177341468 1102 1 0.15170623296527097 1102 2 0.24981476287183568 1102 3 0.18028557452399596 1102 4 0.20512347305908918 1102 5 0.20762147300340672 1102 6 0.1322118483252126 1102 7 0.1913875677978183 1102 8 0.20259140861912783 1102 9 0.22915254090549553 1102 10-11 0.2949193896123532 1102 12-13 0.2615413934406945 1102 14-15 0.27660146027439225 1102 16-17 0.3018320404108721 1102 18-19 0.16769379558517272 1102 20-21 0.15579840683439983 1102 22-23 0.2503249482944838 1102 24-25 0.26290467947396934 1102 26-27 0.2837505018787496 1102 28-29 0.17997215114699117 1102 30-31 0.27831245891933776 1102 32-33 0.22690239988531857 1102 34-35 0.3116086330558616 1102 36-37 0.405838167256654 1102 38-39 0.31291687241152744 1102 40-41 0.29580791881817703 1102 42-43 0.5537861848224637 1102 44-45 0.7482395212095128 1102 46-47 0.6948357062239694 1102 48-49 0.7875620970146286 1102 50-51 0.44407124792821406 1102 52-53 0.5865773007685604 1102 54-55 0.440568880665289 1102 56-57 0.5528711104754152 1102 58-59 0.6644422229155609 1102 60-61 0.9380462629220965 1102 62-63 0.8394824693831815 1102 64-65 0.635083343107631 1102 66-67 0.3257170515967829 1102 68-69 0.3987709927790455 1102 70-71 0.5692123510978817 1102 72-73 0.6485210258644187 1102 74-75 0.4860614952811311 1102 76-77 0.5974094286147285 1102 78-79 0.688280554129328 1102 80-81 0.7929299213276515 1102 82-83 0.6511499436660522 1102 84-85 0.7942578134559213 1102 86-87 0.9206489579671384 1102 88-89 0.7785999804139756 1102 90-91 0.5629483284870211 1102 92-93 0.7501359286541778 1102 94-95 0.6450475241090494 1102 96-97 0.8062862527447585 1102 98-99 0.7761615415736216 1102 100-101 1.7625805277361088 1103 1 0.18382890332944868 1103 2 0.2744744423302592 1103 3 0.22570786562141976 1103 4 0.1802688586469543 1103 5 0.2734368965179428 1103 6 0.27671982108325466 1103 7 0.1903761897952947 1103 8 0.18225129198440726 1103 9 0.2031120042226533 1103 10-11 0.19455969791951588 1103 12-13 0.26487323145709496 1103 14-15 0.2647948455360023 1103 16-17 0.1986996232990137 1103 18-19 0.09553319854590114 1103 20-21 0.08889126601633279 1103 22-23 0.0529516764050868 1103 24-25 0.051632300696603295 1103 26-27 0.08009241289379787 1103 28-29 0.053181237236465506 1103 30-31 0.11436604564091368 1103 32-33 0.10749291413840467 1103 34-35 0.17034563399383273 1103 36-37 0.0992580930345568 1103 38-39 -0.1339258338402587 1103 40-41 -0.16694115340562377 1103 42-43 0.05190942612583882 1103 44-45 0.14959876430727093 1103 46-47 0.04427781706774425 1103 48-49 -0.02859425876204824 1103 50-51 0.023780884566193095 1103 52-53 0.10843366877965366 1103 54-55 -4.9772733838437944E-5 1103 56-57 -0.11743882794471716 1103 58-59 0.049914675099401506 1103 60-61 0.12442610061224002 1103 62-63 0.044764472564128965 1103 64-65 0.17556904923706895 1103 66-67 -0.11999030128442101 1103 68-69 0.05666463496329399 1103 70-71 -0.01766542713302499 1103 72-73 -0.051007541486015384 1103 74-75 0.06790399762605759 1103 76-77 0.2754975979389833 1103 78-79 0.012930853872408221 1103 80-81 0.06408281068778976 1103 82-83 0.23573295414686157 1103 84-85 0.6591242544656701 1103 86-87 0.4223956240900506 1103 88-89 0.3534827339721538 1103 90-91 0.4396083341964143 1103 92-93 0.5938449943529669 1103 94-95 0.6086122237542497 1103 96-97 0.8116575671283464 1103 98-99 0.7521413140135706 1103 100-101 0.8703705852378398 1104 1 0.24114307782942745 1104 2 0.3051208459080499 1104 3 0.12784874101998867 1104 4 0.20406860190754372 1104 5 0.23410441898264622 1104 6 0.17488868935937774 1104 7 0.16688453382609936 1104 8 0.16912196985926897 1104 9 0.12612165265529995 1104 10-11 0.17446486997632604 1104 12-13 0.21091447540093355 1104 14-15 0.3543612872106081 1104 16-17 0.4568279101845931 1104 18-19 0.23652454394322575 1104 20-21 0.2402627513780473 1104 22-23 0.25733943573259666 1104 24-25 0.18685901815901929 1104 26-27 0.2696282114775883 1104 28-29 0.5381913825840954 1104 30-31 0.34958158251614435 1104 32-33 0.2267011629271778 1104 34-35 0.2446360808032253 1104 36-37 0.3113103519272613 1104 38-39 0.3975897568674327 1104 40-41 0.18375991082063337 1104 42-43 0.21846414888868537 1104 44-45 0.13267798083797544 1104 46-47 -0.003911755999467914 1104 48-49 0.1693613501658504 1104 50-51 0.21599836769179603 1104 52-53 0.36395729294628865 1104 54-55 0.07208737580233304 1104 56-57 0.032742546025218644 1104 58-59 0.10377901730819161 1104 60-61 0.2602006668468668 1104 62-63 0.20606804621107244 1104 64-65 0.10407540560793649 1104 66-67 0.04987635986096706 1104 68-69 0.04137456084014346 1104 70-71 0.1636472566219993 1104 72-73 0.24482913912187598 1104 74-75 0.15993306715274613 1104 76-77 0.11812669231142081 1104 78-79 0.0920231558310789 1104 80-81 0.5435494552693463 1104 82-83 0.3017133260049114 1104 84-85 0.5105814576624894 1104 86-87 0.5597089703983862 1104 88-89 0.4756803404983039 1104 90-91 0.51536633659817 1104 92-93 0.6034329690103029 1104 94-95 0.8263819036581772 1104 96-97 1.1678839119045286 1104 98-99 0.9970065258235827 1104 100-101 1.312841080910541 1105 1 -0.08876330009600153 1105 2 0.040255675912270306 1105 3 0.10966289346633218 1105 4 0.1646988260736606 1105 5 0.16001670107820587 1105 6 0.08549779733628071 1105 7 -0.034705243429463906 1105 8 0.10357313710366611 1105 9 0.04197719283251189 1105 10-11 0.008575669339080605 1105 12-13 0.03103904001020652 1105 14-15 0.03090496427089562 1105 16-17 -0.02259175003292313 1105 18-19 0.032099343339417885 1105 20-21 0.10077631382551289 1105 22-23 0.1455785546230075 1105 24-25 0.04798829523269177 1105 26-27 -0.009195177733509752 1105 28-29 0.04233538245028967 1105 30-31 -0.05387394595307171 1105 32-33 0.0954492028848648 1105 34-35 0.2395502152694462 1105 36-37 0.0496521247672419 1105 38-39 0.16403675941392493 1105 40-41 0.15358947599439432 1105 42-43 0.17374298712194758 1105 44-45 0.20859214457415476 1105 46-47 0.3326890811215364 1105 48-49 0.17330129656000537 1105 50-51 0.2228751369566595 1105 52-53 0.38208292800000265 1105 54-55 0.14149378090701958 1105 56-57 0.18327463217926976 1105 58-59 0.22187642775255512 1105 60-61 0.3561808552618153 1105 62-63 0.36824456082723955 1105 64-65 0.24021126842378848 1105 66-67 0.3500191554771632 1105 68-69 0.5362516897074379 1105 70-71 0.5814162162419905 1105 72-73 0.7801241176502636 1105 74-75 0.49866789381183096 1105 76-77 0.33675639798101287 1105 78-79 0.5117592661872123 1105 80-81 0.42262226639862277 1105 82-83 0.6279185168241774 1105 84-85 0.6509357496847521 1105 86-87 0.8821665603094502 1105 88-89 0.8117679078714914 1105 90-91 0.8429907025898125 1105 92-93 0.7781226028430375 1105 94-95 1.0241433785299137 1105 96-97 0.9439450526805153 1105 98-99 0.8632162325803456 1105 100-101 1.2721999432288023 1106 1 -0.029904061997445552 1106 2 -0.029197284424959946 1106 3 -0.02687536708515026 1106 4 -0.006923654722029937 1106 5 0.13750106764094028 1106 6 -0.16451875059174625 1106 7 -0.1366291577982892 1106 8 -0.14072697263522116 1106 9 -0.05680174426794338 1106 10-11 -0.1376914664758644 1106 12-13 -0.1470036013874889 1106 14-15 -0.04080373993924269 1106 16-17 -0.13877105118779554 1106 18-19 0.0893597467604863 1106 20-21 -0.06419912556552276 1106 22-23 -0.2555001090011544 1106 24-25 -0.27606607777502745 1106 26-27 -0.30861295195008864 1106 28-29 -0.39751006861138904 1106 30-31 -0.26290280031388846 1106 32-33 -0.17536373585296872 1106 34-35 0.07089678113891296 1106 36-37 -0.28972758921488406 1106 38-39 0.10767930674970927 1106 40-41 0.2641098212364241 1106 42-43 0.26919034729196056 1106 44-45 0.2536745793840325 1106 46-47 0.5350269114268897 1106 48-49 0.3571509542791773 1106 50-51 0.4768523008159775 1106 52-53 0.13937337897458235 1106 54-55 0.19571872821492775 1106 56-57 0.1988997954811964 1106 58-59 0.23016911705213516 1106 60-61 0.3191966715972292 1106 62-63 0.34332795367504687 1106 64-65 0.4226364979174235 1106 66-67 0.2569965022346423 1106 68-69 0.2002462288911886 1106 70-71 0.5476588785423289 1106 72-73 0.5077047234786214 1106 74-75 0.3431722398202943 1106 76-77 0.1522463472920954 1106 78-79 0.2497128100566428 1106 80-81 0.4321904525715219 1106 82-83 0.36315101521293514 1106 84-85 0.7411097642120907 1106 86-87 0.8326190154994002 1106 88-89 0.6564799739238545 1106 90-91 0.7606584463233759 1106 92-93 0.7642447700990438 1106 94-95 0.7712627326381529 1106 96-97 0.7956954315409739 1106 98-99 0.5382375706980653 1106 100-101 0.8326092431643524 1107 1 0.06296939708835225 1107 2 0.13211664787175792 1107 3 0.07205996122607417 1107 4 0.20493596477747644 1107 5 0.04389174160693443 1107 6 0.03179116307828167 1107 7 0.13506966375705787 1107 8 -0.01649368430580722 1107 9 0.0647123614642382 1107 10-11 0.05931377536438731 1107 12-13 0.0017499193172554328 1107 14-15 0.22738803668151775 1107 16-17 0.12302330969193775 1107 18-19 -0.04627044616884035 1107 20-21 -0.07344505141833224 1107 22-23 0.0013372846726795729 1107 24-25 0.13930151200074192 1107 26-27 0.17223203481311344 1107 28-29 0.25573466399130496 1107 30-31 0.1779478178143492 1107 32-33 0.26842131697082294 1107 34-35 0.20614251663572247 1107 36-37 0.25903439175656473 1107 38-39 0.043740400450680283 1107 40-41 -0.06434923812291515 1107 42-43 -0.12077881679843472 1107 44-45 0.03464862927845758 1107 46-47 -0.0857387691486835 1107 48-49 0.06112313762725563 1107 50-51 0.10851476085002787 1107 52-53 0.3050807013354273 1107 54-55 0.11424988490963983 1107 56-57 0.20689166304982365 1107 58-59 0.01630547187571807 1107 60-61 0.2625512202243776 1107 62-63 0.36123926364159686 1107 64-65 0.2712162306434962 1107 66-67 0.19947043403784193 1107 68-69 0.44642434746887716 1107 70-71 0.34126582512631387 1107 72-73 0.531215011515453 1107 74-75 0.5609756373224002 1107 76-77 0.4670407539982655 1107 78-79 0.343480325452564 1107 80-81 0.44713339864789603 1107 82-83 0.3779285013856821 1107 84-85 0.6526294748709667 1107 86-87 0.6545290793391629 1107 88-89 0.6941083320328438 1107 90-91 0.7383046150563466 1107 92-93 1.0177114965750924 1107 94-95 0.8860998372528783 1107 96-97 0.9510345207932609 1107 98-99 0.94231929687448 1107 100-101 1.612280764496564 1108 1 0.08345726008799659 1108 2 0.15542583006698152 1108 3 0.057430270873727096 1108 4 0.19412631639860223 1108 5 0.1505419663195653 1108 6 0.11373292814400315 1108 7 0.13034679964099638 1108 8 0.048442185776927715 1108 9 0.12162163897887979 1108 10-11 0.1927837267408279 1108 12-13 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-1.596790619832344 2316 76-77 -1.7019171087636273 2316 78-79 -1.8777914664771345 2316 80-81 -1.815551845814955 2316 82-83 -1.7993158512821594 2316 84-85 -1.7897583336165113 2316 86-87 -1.571060729911995 2316 88-89 -1.8627625016883478 2316 90-91 -1.5305840986380197 2316 92-93 -2.0680756154824884 2316 94-95 -2.415180655301249 2316 96-97 -2.2921883006786636 2316 98-99 -2.8220661787818173 2316 100-101 -3.8205320827922797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 20.0 4 26.0 5 65.0 6 161.0 7 363.0 8 793.0 9 1473.0 10 2349.0 11 3596.0 12 5032.0 13 7119.0 14 9070.0 15 12336.0 16 16100.0 17 20565.0 18 17496.0 19 10980.0 20 9918.0 21 10159.0 22 10798.0 23 11903.0 24 13474.0 25 16107.0 26 19075.0 27 23529.0 28 28133.0 29 34125.0 30 41230.0 31 50077.0 32 61112.0 33 76119.0 34 97866.0 35 127832.0 36 164160.0 37 173053.0 38 80382.0 39 3473.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.64952609066399 19.25529887801652 15.344091890041653 18.751083141277835 2 16.06262732328627 24.106226092534015 35.944759259307155 23.88638732487256 3 23.50814335617312 25.815025293888432 30.429823684183305 20.24700766575514 4 12.460207108370277 17.24233644722857 38.43788602229695 31.8595704221042 5 11.837041864506718 41.09780108755995 32.63662601247556 14.428531035457764 6 28.95508801631627 37.802061773911724 15.845609183591666 17.39724102618034 7 26.82374927698266 35.78373355377999 19.66995066672758 17.722566502509764 8 27.26872280872948 29.958045599011783 18.84905122022914 23.924180372029593 9 25.896735452804965 18.623202585363458 21.4942387047667 33.98582325706488 10-11 26.210380589430454 26.08129344030398 25.179536734452864 22.528789235812695 12-13 30.165879494060217 24.49332318442424 25.437068287044024 19.903729034471525 14-15 23.990360909877698 25.936324422361444 28.87386773878773 21.199446928973128 16-17 20.234615354776313 29.06344363999038 28.669630858164467 22.032310147068838 18-19 20.033153829112095 28.57548013140847 31.319248271837264 20.072117767642172 20-21 22.775714203206878 24.33338016962784 30.73752509549001 22.15338053167527 22-23 21.155545326261326 25.28341035474675 31.607915749693767 21.95312856929815 24-25 23.325724590520046 25.29784866245025 31.34352353178992 20.032903215239784 26-27 22.07500181026368 23.857637416253755 34.12859940761276 19.938761365869805 28-29 19.82784147554105 28.330857116010673 31.954711730804792 19.886589677643485 30-31 20.905968398816775 25.24749978772494 31.504765090170565 22.341766723287716 32-33 21.004905732435205 26.00628723427642 31.926960148521143 21.06184688476723 34-35 23.084784174413848 24.80570805196865 31.565184296650433 20.544323476967065 36-37 22.40948744030481 24.089529851904214 33.359481406134165 20.14150130165681 38-39 21.26681171738557 25.943662967413584 32.675418558101605 20.114106757099236 40-41 20.98272856889177 26.711885358246857 31.506828059782805 20.798558013078566 42-43 20.505554897037747 27.224922145235503 29.53166452947705 22.737858428249698 44-45 21.981517958355067 25.141395376903482 29.908063911313786 22.969022753427666 46-47 23.63617555912815 27.02926129139315 29.44666893665441 19.887894212824282 48-49 23.07291799112721 26.823402339613306 28.959319899298613 21.144359769960868 50-51 20.260002336072976 27.77987296417921 31.172006087582048 20.788118612165768 52-53 22.35388128444305 28.83070546787384 27.500221969937943 21.315191277745164 54-55 20.606516699057497 26.9205952671888 28.88852683568517 23.58436119806853 56-57 23.129334395486538 26.3059656455512 28.16779434230991 22.396905616652354 58-59 25.006563770315893 25.88082779813852 27.46199587765046 21.65061255389513 60-61 21.96667573396629 27.096884886823286 30.727195992914574 20.209243386295846 62-63 21.50788440374803 27.070741730115916 29.925645509827646 21.49572835630841 64-65 20.768068491172407 29.682999851274833 28.43517414854842 21.113757509004337 66-67 22.134644428506405 26.90983476545798 27.44791594830559 23.507604857730023 68-69 21.34108233776959 27.4233382152521 28.296394247553796 22.939185199424514 70-71 23.559480995941616 27.315597016726606 27.453606167914295 21.671315819417483 72-73 22.663867407886944 25.903157484597898 29.936365854100057 21.4966092534151 74-75 20.8930430944713 30.291856600026208 27.57737557497224 21.23772473053025 76-77 21.961378951261207 27.105722987377167 27.496097120705215 23.436800940656408 78-79 22.075229985784027 27.72127214402528 28.17288137638873 22.030616493801958 80-81 23.353622788564333 27.251224264579093 27.61643445873711 21.778718488119463 82-83 22.3313635587682 26.6763632225213 29.303011910037576 21.68926130867293 84-85 21.354628565762216 28.187354356950234 28.475180591992515 21.98283648529504 86-87 21.462736081260143 28.570326287212406 27.194681743451536 22.772255888075907 88-89 21.109560257559547 28.49415282217258 26.10138270308169 24.29490421718619 90-91 22.129177060582606 26.625515024374046 26.952241528374582 24.293066386668766 92-93 23.132606374998062 28.204227234551848 27.352856470279846 21.310309920170248 94-95 22.06123918376498 27.733969121723874 27.425876185097202 22.77891550941395 96-97 19.68068568402596 27.978381772244475 29.495305888090783 22.845626655638775 98-99 21.359202785489853 29.006535068313326 26.03522226735907 23.599039878837747 100-101 20.51311908088806 27.612387655471025 26.927538856975282 24.94695440666563 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1255.0 1 936.5 2 813.0 3 1060.0 4 909.5 5 794.5 6 1331.0 7 2693.5 8 3992.0 9 4456.5 10 3903.5 11 3011.5 12 2764.5 13 2850.5 14 2969.5 15 3094.0 16 3158.5 17 3279.0 18 3487.5 19 3623.5 20 3798.0 21 4140.5 22 4652.5 23 5241.0 24 5934.0 25 6805.0 26 7898.5 27 9175.0 28 10399.0 29 11825.5 30 13744.0 31 15872.0 32 18619.0 33 20005.5 34 20368.0 35 21078.0 36 22499.0 37 24297.5 38 24884.0 39 24765.5 40 24976.5 41 26489.0 42 27519.0 43 28269.0 44 29449.5 45 31268.5 46 33946.0 47 55194.0 48 101077.5 49 96194.0 50 51540.0 51 33576.0 52 28302.0 53 27633.5 54 27524.0 55 26226.0 56 24639.5 57 23016.0 58 22588.5 59 21161.0 60 18144.5 61 15831.5 62 13946.0 63 10824.5 64 7799.0 65 6128.0 66 4567.0 67 3580.5 68 2760.0 69 1916.0 70 1460.0 71 1191.5 72 1054.5 73 1004.5 74 846.0 75 731.0 76 561.5 77 375.5 78 281.0 79 207.0 80 157.5 81 137.5 82 101.5 83 52.5 84 28.0 85 15.5 86 9.5 87 5.0 88 1.5 89 0.0 90 0.5 91 2.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021378038720110615 2 0.011033826436186124 3 0.00905118574843393 4 0.0 5 0.0035342725303408678 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.620176903270409E-4 14-15 8.620176903270409E-5 16-17 0.0 18-19 0.0018964389187194899 20-21 0.0 22-23 1.7240353806540818E-4 24-25 0.0 26-27 0.0014654300735559697 28-29 0.002887759262595587 30-31 0.0015085309580723217 32-33 0.008577076018754057 34-35 0.002025741572268546 36-37 7.758159212943367E-4 38-39 0.0012930265354905615 40-41 2.5860530709811226E-4 42-43 0.0048703999503477815 44-45 0.002068842456784898 46-47 3.4480707613081637E-4 48-49 4.7410972967987247E-4 50-51 2.1550442258176024E-4 52-53 1.7240353806540818E-4 54-55 0.001594732727105026 56-57 0.013878484814265358 58-59 0.02530021921109865 60-61 0.019266095378809363 62-63 0.013145769777487375 64-65 0.018231674150416915 66-67 0.0018102371496867859 68-69 5.172106141962245E-4 70-71 8.620176903270409E-5 72-73 0.0 74-75 0.0011206229974251533 76-77 0.0024136495329157145 78-79 0.009137387517466634 80-81 0.016292134347181072 82-83 0.017843766189769746 84-85 0.029437904124668443 86-87 0.012068247664578573 88-89 0.042454371248606765 90-91 0.046247249086045744 92-93 0.030256820930479135 94-95 0.03051542623757725 96-97 0.050384933999615536 98-99 0.05447951802866898 100-101 0.05939301886353312 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1160069.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.132459825013036 #Duplication Level Percentage of deduplicated Percentage of total 1 73.97081860668612 31.1657254316953 2 13.863625248170965 11.682172675951993 3 4.971864338196071 6.2843062345338305 4 2.316928259461688 3.904715472368278 5 1.2943217285709057 2.7266479114827558 6 0.8041081959874925 2.032743375744404 7 0.5247441890586642 1.5476134424746248 8 0.3513761871608354 1.184347447121613 9 0.25452660341706024 0.9651448703579686 >10 1.4453006859974982 11.230796078158695 >50 0.11600449792544597 3.3580601578438065 >100 0.07188244399226879 5.653902005357252 >500 0.006943190461256674 2.13145211200265 >1k 0.004901075619710593 4.868879165467161 >5k 0.0016336918732368642 4.796520096069258 >10k+ 0.00102105742077304 6.46697352337041 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 31177 2.687512553132615 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12675 1.0926074224895244 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10703 0.9226175339570319 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10603 0.9139973570537615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10005 0.8624486991722043 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7923 0.6829766160461146 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7635 0.6581505065646958 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7215 0.62194576357096 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6987 0.6022917602315034 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6880 0.5930681709450042 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6718 0.579103484361706 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6437 0.5548807872635162 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5953 0.5131591310516875 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4992 0.43031923101125885 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4841 0.4173027638873205 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3712 0.31998096664939757 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3321 0.28627607495761026 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3099 0.26713928223234995 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3005 0.2590363159432758 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2999 0.25851910532907957 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2608 0.2248142136372923 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2558 0.22050412518565707 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2555 0.22024551987855895 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2532 0.21826287919080675 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2373 0.2045567979146068 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2148 0.1851613998822484 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2121 0.18283395211836537 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1975 0.17024849383959056 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1548 0.13344033846262593 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1504 0.12964746062518695 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1458 0.12568217924968256 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1400 0.12068247664578573 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1364 0.11757921296060839 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.10464894760570276 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1165 0.10042506092310026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2930265354905613E-4 0.0 24-25 0.0 0.0 0.0 3.4480707613081637E-4 0.0 26-27 0.0 0.0 0.0 0.001034421228392449 0.0 28-29 0.0 0.0 0.0 0.002025741572268546 0.0 30-31 0.0 0.0 0.0 0.004353189336151557 0.0 32-33 0.0 0.0 0.0 0.006896141522616327 0.0 34-35 0.0 0.0 0.0 0.010042506092310027 0.0 36-37 0.0 0.0 0.0 0.014524998082010639 0.0 38-39 0.0 0.0 0.0 0.024179596213673497 0.0 40-41 0.0 0.0 0.0 0.03650644918535018 0.0 42-43 0.0 0.0 0.0 0.05344509680027654 0.0 44-45 0.0 0.0 0.0 0.07107335856746452 0.0 46-47 0.0 0.0 0.0 0.10417483787602289 0.0 48-49 0.0 0.0 0.0 0.13973306760201332 0.0 50-51 0.0 0.0 0.0 0.16580910273440633 0.0 52-53 0.0 0.0 0.0 0.20119492892233135 0.0 54-55 0.0 0.0 0.0 0.2513643584993651 0.0 56-57 0.0 0.0 0.0 0.32653230109588305 0.0 58-59 0.0 0.0 0.0 0.4105359250182532 0.0 60-61 0.0 0.0 0.0 0.5070819063348818 0.0 62-63 0.0 0.0 0.0 0.6064294451450734 0.0 64-65 0.0 0.0 0.0 0.7118973095565868 0.0 66-67 0.0 0.0 0.0 0.8460703630559906 0.0 68-69 0.0 0.0 0.0 1.0123535755200768 0.0 70-71 0.0 0.0 0.0 1.1566122359963071 0.0 72-73 0.0 0.0 0.0 1.3089738627616114 0.0 74-75 0.0 0.0 0.0 1.472110710656004 0.0 76-77 0.0 0.0 0.0 1.6514965920130613 0.0 78-79 0.0 0.0 0.0 1.880793297640054 0.0 80-81 0.0 0.0 0.0 2.1547425196259877 0.0 82-83 0.0 0.0 0.0 2.426967706231267 0.0 84-85 0.0 0.0 0.0 2.762163285114937 0.0 86-87 0.0 0.0 0.0 3.131624067189107 0.0 88-89 0.0 0.0 0.0 3.550564664688049 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCG 55 0.0025816273 34.540016 5 TAAAGCG 95 3.4946243E-5 29.995277 5 GTAAGCG 230 0.0 26.859808 94-95 ATACGTC 145 7.898525E-7 26.20277 5 GTACATG 9335 0.0 25.1478 1 TACATGG 9290 0.0 24.852886 2 AAAGCGT 160 1.8621304E-6 23.746262 6 ACATGGG 9440 0.0 23.648697 3 GAGTACT 9300 0.0 23.288662 12-13 AGTACTT 9390 0.0 22.3573 12-13 TAAGGTG 535 0.0 22.192766 5 GTACTTT 9805 0.0 22.063074 14-15 ATGGGAG 2215 0.0 21.655731 5 GTAGTAC 110 0.002877851 21.590302 3 GTATAGG 310 1.0913936E-11 21.461185 1 CATGGGA 6385 0.0 21.421843 4 GTAAGGT 470 0.0 21.220064 4 TGGGAGT 540 0.0 21.107786 6 CAGTCCG 160 4.9920767E-5 20.77977 9 CATGGGC 1100 0.0 20.724009 4 >>END_MODULE