FastQCFastQC Report
Thu 26 May 2016
SRR936379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626949
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64080.39386606464001017No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61600.37862280870512843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.32736121414992114No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46310.28464321868724834No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32050.19699449706167801No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31480.19349100678632214No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28770.17683406179296338No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18480.11358684261153854No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16540.1016626827269939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG72550.025.6007291
TACATGG74100.025.0652222
ACATGGG73300.024.2378443
GAGTACT62350.021.56501612-13
AGTACTT63950.021.06261612-13
GTACTTT65650.020.30071314-15
GTATAGG4050.019.9391631
GTGTAGC8350.019.911071
CATGGGG36200.019.5525784
CATGGGA34200.019.0292134
ATGGGTA6600.018.7135335
GTATCAA141400.018.711941
GAGTACA68800.018.2966021
AGAGTAC109150.018.25918410-11
TACTTTT73000.018.22419414-15
ACCGCGC2352.525423E-618.1939818
AGTACAT57750.018.0960462
ACTTTTT76150.017.84497616-17
GTATATA9700.017.6296541
GGTATCA115300.017.2622621
Produced by FastQC (version 0.11.5)