##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936379_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1626949 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85260816411578 33.0 31.0 34.0 30.0 34.0 2 31.9599630965691 33.0 31.0 34.0 30.0 34.0 3 31.98424720135665 33.0 31.0 34.0 30.0 34.0 4 35.62131142402128 37.0 35.0 37.0 33.0 37.0 5 35.63628300579797 37.0 35.0 37.0 33.0 37.0 6 35.64350142506004 37.0 35.0 37.0 33.0 37.0 7 35.58392610954615 37.0 35.0 37.0 33.0 37.0 8 35.54565324420126 37.0 35.0 37.0 33.0 37.0 9 37.13049640769317 39.0 37.0 39.0 34.0 39.0 10-11 37.01379576126848 39.0 37.0 39.0 33.0 39.0 12-13 36.932251103138455 39.0 37.0 39.0 33.0 39.0 14-15 38.112092942065175 40.0 38.0 41.0 33.0 41.0 16-17 38.01274225559621 40.0 38.0 41.0 33.0 41.0 18-19 37.97921231704251 40.0 38.0 41.0 33.0 41.0 20-21 37.845600876241356 40.0 38.0 41.0 33.0 41.0 22-23 37.96726510787984 40.0 38.0 41.0 33.0 41.0 24-25 38.00250960540251 40.0 38.0 41.0 33.0 41.0 26-27 37.906639667254474 40.0 38.0 41.0 33.0 41.0 28-29 37.775590076886246 40.0 37.5 41.0 33.0 41.0 30-31 37.56413692131714 40.0 37.0 41.0 32.5 41.0 32-33 37.368023521327345 39.5 36.5 41.0 32.0 41.0 34-35 37.1717540008937 39.0 36.0 41.0 31.5 41.0 36-37 36.96546203968287 39.0 36.0 41.0 31.0 41.0 38-39 36.754102003197396 39.0 36.0 41.0 31.0 41.0 40-41 36.54485481720693 39.0 35.0 40.0 30.0 41.0 42-43 36.2928970115228 39.0 35.0 40.0 30.0 41.0 44-45 36.01337657173028 38.5 35.0 40.0 29.5 41.0 46-47 35.81071502548635 39.0 35.0 40.0 28.5 41.0 48-49 35.62084582860311 39.0 35.0 40.0 28.0 41.0 50-51 34.64105973819708 38.0 34.0 39.5 26.0 40.5 52-53 34.740949470450516 38.0 34.0 39.5 26.5 40.5 54-55 35.16459827566814 38.0 35.0 40.0 27.0 41.0 56-57 35.02248441715137 38.0 34.5 40.0 26.5 41.0 58-59 34.768386409162176 38.0 34.0 40.0 26.0 41.0 60-61 34.45715385055094 37.5 34.0 40.0 26.0 41.0 62-63 34.100616860147426 37.0 34.0 40.0 25.5 41.0 64-65 33.73275683503294 36.5 33.5 39.5 24.5 41.0 66-67 33.260724829112654 36.0 33.0 39.0 23.0 41.0 68-69 32.860059534748785 35.0 32.5 39.0 22.0 40.0 70-71 32.450147484647644 35.0 32.0 38.0 21.5 40.0 72-73 32.065042604285686 35.0 32.0 37.0 20.5 39.5 74-75 31.648074094516794 35.0 31.0 37.0 19.5 39.0 76-77 31.20355401429301 35.0 31.0 36.0 18.0 39.0 78-79 30.72854465628609 34.5 31.0 36.0 15.5 38.0 80-81 30.2775136159769 34.0 30.0 35.0 9.5 37.0 82-83 29.87877063140885 34.0 30.0 35.0 7.0 37.0 84-85 29.525852377671335 34.0 29.5 35.0 4.5 36.0 86-87 29.194125322920385 34.0 29.0 35.0 2.0 36.0 88-89 28.787947563199584 34.0 29.0 35.0 2.0 35.5 90-91 28.383812276844573 34.0 29.0 35.0 2.0 35.0 92-93 27.9333073747241 34.0 27.5 35.0 2.0 35.0 94-95 27.436766303061745 33.0 27.0 35.0 2.0 35.0 96-97 26.852240604960574 33.0 25.5 35.0 2.0 35.0 98-99 26.102998618887256 33.0 24.5 35.0 2.0 35.0 100-101 23.08356930672074 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3593778216958299 1101 2 0.3282627164692933 1101 3 0.30523928079034945 1101 4 0.28507017276825763 1101 5 0.28644314071522814 1101 6 0.31654420047424026 1101 7 0.3088102368133434 1101 8 0.4069429329879455 1101 9 0.5010170876782709 1101 10-11 0.5436150317741308 1101 12-13 0.47327983784521876 1101 14-15 0.4188930585030164 1101 16-17 0.564158605275459 1101 18-19 0.6004246811306757 1101 20-21 0.5529852911082571 1101 22-23 0.5061340501683773 1101 24-25 0.5437636595347044 1101 26-27 0.5963187752875072 1101 28-29 0.48880788884849835 1101 30-31 0.5417237073325012 1101 32-33 0.4548279386571821 1101 34-35 0.34496561144266025 1101 36-37 0.3020291767025043 1101 38-39 0.28589683848341707 1101 40-41 0.39447844178290126 1101 42-43 0.542379692375917 1101 44-45 0.28210095019860404 1101 46-47 0.544605637047006 1101 48-49 0.6799061476256085 1101 50-51 0.6796800224848525 1101 52-53 0.6376650098142704 1101 54-55 0.5404780291178142 1101 56-57 0.5257168265596093 1101 58-59 0.3865708509806183 1101 60-61 0.427403094031078 1101 62-63 0.4466930665922959 1101 64-65 0.48472256054916585 1101 66-67 0.366667004488896 1101 68-69 0.35928195130990304 1101 70-71 0.3784471903407578 1101 72-73 0.11278773541992848 1101 74-75 0.07206266012115847 1101 76-77 0.1092596992037862 1101 78-79 0.09771965563597007 1101 80-81 0.15268572632457023 1101 82-83 0.02573645329838925 1101 84-85 0.06119147145933468 1101 86-87 0.16409826774408387 1101 88-89 0.09764364831492145 1101 90-91 0.07405949187731053 1101 92-93 -0.10753590441338545 1101 94-95 -0.15414206142945375 1101 96-97 -0.2007468712511269 1101 98-99 -0.42488860781610427 1101 100-101 -0.015991685316461002 1102 1 0.1624907672178466 1102 2 0.19562948123638435 1102 3 0.16759005103664393 1102 4 0.1564737556140301 1102 5 0.08702928878916083 1102 6 0.16967896204738508 1102 7 0.17811577093581832 1102 8 0.20195769766312566 1102 9 0.18746076845212656 1102 10-11 0.2556163926988262 1102 12-13 0.29652138709557363 1102 14-15 0.2654884881251576 1102 16-17 0.2391434410962887 1102 18-19 0.244955824033255 1102 20-21 0.2166451027564804 1102 22-23 0.1255302560293856 1102 24-25 0.037467946682504305 1102 26-27 -0.10974146521389372 1102 28-29 -0.12817510403171184 1102 30-31 0.03948648499756757 1102 32-33 0.03474544761142084 1102 34-35 0.08857844351076238 1102 36-37 -0.21466982889812414 1102 38-39 -0.12159827412056501 1102 40-41 -0.32250784113015385 1102 42-43 -0.24547559526826035 1102 44-45 -0.11989197414187203 1102 46-47 -0.07959036117637908 1102 48-49 0.016048803449784543 1102 50-51 0.16186945578713363 1102 52-53 0.24934648032316886 1102 54-55 -0.04222451875151023 1102 56-57 0.08603657597399916 1102 58-59 0.17408642967781418 1102 60-61 -0.01126677702313117 1102 62-63 0.14192113506362602 1102 64-65 0.007060578001485851 1102 66-67 0.1217570900271383 1102 68-69 0.2588859462453428 1102 70-71 0.2701757344307154 1102 72-73 -0.045667296489472875 1102 74-75 -0.019341632953921106 1102 76-77 0.13090759309970323 1102 78-79 0.055523026039615786 1102 80-81 0.17521305122236086 1102 82-83 0.09394118420895481 1102 84-85 -0.23056511624393394 1102 86-87 -0.15190207588156923 1102 88-89 -0.024562271707512906 1102 90-91 -0.08657401178049895 1102 92-93 0.03987976173079133 1102 94-95 -0.20227928922167138 1102 96-97 -0.1986870756875021 1102 98-99 -0.12325134647077718 1102 100-101 0.06045718900924513 1103 1 0.13974654010201348 1103 2 0.02378403131264406 1103 3 0.07427963977718477 1103 4 0.1476125456496149 1103 5 0.29882764589188326 1103 6 0.15813747487749907 1103 7 0.18922004407417603 1103 8 0.17506609142332508 1103 9 0.02854256547136913 1103 10-11 0.24289624713919267 1103 12-13 -0.04117695127704479 1103 14-15 0.07050093026253279 1103 16-17 0.18944352404387388 1103 18-19 0.28006258559909014 1103 20-21 0.30945408250121886 1103 22-23 -0.10703857708542586 1103 24-25 0.08168556367873236 1103 26-27 -0.09996903592001871 1103 28-29 -0.01015367144668744 1103 30-31 0.1453771012178393 1103 32-33 -0.031337687272049664 1103 34-35 0.21534027691991042 1103 36-37 0.23152408114388834 1103 38-39 0.11085777703936373 1103 40-41 0.09387751728367988 1103 42-43 0.028391099939511832 1103 44-45 0.2910968413131414 1103 46-47 0.21045552756682895 1103 48-49 0.2766416110916694 1103 50-51 0.19485289302792808 1103 52-53 0.28627447647012616 1103 54-55 0.2503666566960092 1103 56-57 0.28579576041195764 1103 58-59 0.2821490684812247 1103 60-61 0.19580022321768809 1103 62-63 0.25526833758951994 1103 64-65 0.21561394540575662 1103 66-67 0.16819796980666268 1103 68-69 0.19665064268394872 1103 70-71 0.18994950158882773 1103 72-73 -0.005475179185538082 1103 74-75 -0.12807587960257294 1103 76-77 -0.10009098876420808 1103 78-79 -0.21442412832315938 1103 80-81 -0.12043608050374743 1103 82-83 -0.25290720901610086 1103 84-85 -0.12570050810436584 1103 86-87 0.2120434195295573 1103 88-89 -0.10183490168102338 1103 90-91 -0.06214032971055872 1103 92-93 0.062373808234951866 1103 94-95 0.2681405325748152 1103 96-97 0.17446560137882727 1103 98-99 0.2608728876019448 1103 100-101 0.2911761572483478 1104 1 0.2697649735353096 1104 2 0.2667427349195428 1104 3 0.1975995836515061 1104 4 0.2724269732502407 1104 5 0.21760883828740418 1104 6 0.2989815610469222 1104 7 0.28598181258327315 1104 8 0.4384380340870919 1104 9 0.4149224592727805 1104 10-11 0.48704872189640014 1104 12-13 0.30679399543107166 1104 14-15 0.198751679980532 1104 16-17 0.06398516434431656 1104 18-19 0.023321864219056465 1104 20-21 -0.0495562897440891 1104 22-23 -0.05424178505307253 1104 24-25 0.06255729999809745 1104 26-27 -0.13047430458043152 1104 28-29 -0.06078467262973675 1104 30-31 0.0593301097192267 1104 32-33 0.059984154970770476 1104 34-35 0.19754431026516528 1104 36-37 -0.13443720864619024 1104 38-39 -0.04043724043113173 1104 40-41 -0.1566034027372325 1104 42-43 0.11332025502795773 1104 44-45 0.16684958569369002 1104 46-47 -0.021599800245645895 1104 48-49 0.24716833956994577 1104 50-51 0.13710644902979396 1104 52-53 0.28703685025612913 1104 54-55 0.11002797674992593 1104 56-57 0.12098435919590855 1104 58-59 0.23044260027202057 1104 60-61 0.26891808714043464 1104 62-63 0.11534275913736991 1104 64-65 0.029261976880157192 1104 66-67 0.010997513097713352 1104 68-69 0.012739453314814853 1104 70-71 0.16950778700440594 1104 72-73 0.13451161477270546 1104 74-75 0.15758112097519117 1104 76-77 -0.08404317188798771 1104 78-79 0.020015165153608905 1104 80-81 0.09574420947891937 1104 82-83 -0.14669940644642665 1104 84-85 -0.11645889256525876 1104 86-87 -0.004237566521563707 1104 88-89 0.05260673036563901 1104 90-91 -0.10364967023384253 1104 92-93 -0.5298794726410456 1104 94-95 -0.3017551947204211 1104 96-97 -0.31681469685761243 1104 98-99 -0.2759836617693914 1104 100-101 -0.16765785931422883 1105 1 0.3318009432107658 1105 2 0.2623942376101489 1105 3 0.36494024895484856 1105 4 0.2552021221968488 1105 5 0.2357086105184294 1105 6 0.31051303919546314 1105 7 0.3644014830135447 1105 8 0.37943548396003735 1105 9 0.5219279017763014 1105 10-11 0.4031236887072893 1105 12-13 0.23249011541248166 1105 14-15 0.3230302176503841 1105 16-17 0.36246226797209147 1105 18-19 0.4000788722440376 1105 20-21 0.41695165379100274 1105 22-23 0.16881061429693744 1105 24-25 0.17637183146545965 1105 26-27 0.12030497973801602 1105 28-29 0.11417657599602649 1105 30-31 0.11398387874798033 1105 32-33 0.045062392732525325 1105 34-35 0.1947328136362998 1105 36-37 0.3075647120370846 1105 38-39 0.1513149460204417 1105 40-41 0.15697197982439803 1105 42-43 0.06462974214763051 1105 44-45 0.1750327805096603 1105 46-47 0.25194332686965026 1105 48-49 0.3000140895190029 1105 50-51 0.38810850763447746 1105 52-53 0.48738996878397245 1105 54-55 0.4723719141392877 1105 56-57 0.3434247677838087 1105 58-59 0.5047975055347749 1105 60-61 0.46644168844427014 1105 62-63 0.4913746865237414 1105 64-65 0.47987504604008535 1105 66-67 0.4852872320502186 1105 68-69 0.5482827359464224 1105 70-71 0.42688218345636386 1105 72-73 0.39974511911722743 1105 74-75 0.5141279606168716 1105 76-77 0.3540738206534719 1105 78-79 0.4988483443895717 1105 80-81 0.5016900409889082 1105 82-83 0.44235794899292813 1105 84-85 0.29057385043040895 1105 86-87 0.32981843524474286 1105 88-89 0.23851768989563737 1105 90-91 0.10056439627378566 1105 92-93 -0.18493803815627174 1105 94-95 -0.018161541829435635 1105 96-97 0.02433673687228577 1105 98-99 -0.07591064288550697 1105 100-101 0.06437291134858114 1106 1 0.20046752063554152 1106 2 0.21803060665510898 1106 3 0.14743753451402242 1106 4 0.14336597030715126 1106 5 0.1320360092660735 1106 6 0.32864937105630787 1106 7 0.35980403830633634 1106 8 0.3055491483807842 1106 9 0.4022051465816787 1106 10-11 0.43422285132088234 1106 12-13 0.5051820970286443 1106 14-15 0.3579919180058653 1106 16-17 0.4878898109941261 1106 18-19 0.4082132705305952 1106 20-21 0.3341332461638018 1106 22-23 0.376353636324815 1106 24-25 0.2510186566852184 1106 26-27 0.3454996014267735 1106 28-29 0.2720921829152445 1106 30-31 0.3652545345271321 1106 32-33 0.25992042375894897 1106 34-35 0.15086298780671825 1106 36-37 0.12365457862046014 1106 38-39 0.16984840429242354 1106 40-41 0.22935696933847538 1106 42-43 0.3222916046258817 1106 44-45 0.12500095090218366 1106 46-47 0.18999770492804657 1106 48-49 0.14967333423513907 1106 50-51 -0.01445496565988691 1106 52-53 0.021811606967595765 1106 54-55 0.2499701174025617 1106 56-57 0.2794655513276112 1106 58-59 0.18270061858937225 1106 60-61 0.28620757721913037 1106 62-63 0.3652503130761744 1106 64-65 0.20618712492991875 1106 66-67 0.36066732813398517 1106 68-69 0.4404718395116376 1106 70-71 0.40494950158882403 1106 72-73 0.22257096356860728 1106 74-75 0.38060112111840994 1106 76-77 0.32928896797696083 1106 78-79 0.2788455183963805 1106 80-81 0.4033166231732501 1106 82-83 0.3327128342427308 1106 84-85 0.4035749064594434 1106 86-87 0.32710830777757494 1106 88-89 0.3786914141084523 1106 90-91 0.38808317425483807 1106 92-93 0.28134280607201134 1106 94-95 0.2847807632885875 1106 96-97 0.6128614196917361 1106 98-99 0.8459305660446326 1106 100-101 0.4427551046167686 1107 1 0.2997142867667222 1107 2 0.3333662040145917 1107 3 0.27523697741095887 1107 4 0.3858019606913956 1107 5 0.33161169515152267 1107 6 0.3554956335052708 1107 7 0.3618340302932097 1107 8 0.2948344538335306 1107 9 0.45578930348631985 1107 10-11 0.5527232519771985 1107 12-13 0.5220249252806397 1107 14-15 0.17124715515623734 1107 16-17 0.15608991020425833 1107 18-19 0.1924999115043846 1107 20-21 0.2620196887173165 1107 22-23 0.37082610551243533 1107 24-25 0.2667808576238997 1107 26-27 0.2674807515011821 1107 28-29 0.3068592298874222 1107 30-31 0.2709965118614335 1107 32-33 -0.047959150400622264 1107 34-35 0.0806107651181236 1107 36-37 0.1779271012289243 1107 38-39 0.034100703296509494 1107 40-41 0.23897501031989066 1107 42-43 0.2710182807047943 1107 44-45 0.217127481981656 1107 46-47 0.12150302793335044 1107 48-49 0.2055530610256966 1107 50-51 0.10656231979819353 1107 52-53 0.28449028060441606 1107 54-55 0.36879943781314495 1107 56-57 0.24152072742559483 1107 58-59 0.37229347545967784 1107 60-61 0.38972266860692173 1107 62-63 0.26540981244657047 1107 64-65 0.2503295296309531 1107 66-67 0.13987441021129854 1107 68-69 0.41284181699856504 1107 70-71 0.40578515618492617 1107 72-73 0.262300806740285 1107 74-75 0.3575897119529188 1107 76-77 0.21688159590079792 1107 78-79 0.15889283399908294 1107 80-81 0.2651840631701994 1107 82-83 0.3371536325027371 1107 84-85 0.29039903741681883 1107 86-87 0.2762722716040322 1107 88-89 0.048329297116808334 1107 90-91 -0.026195453592684714 1107 92-93 0.19427675501694708 1107 94-95 0.1819654849278436 1107 96-97 0.23936884137296488 1107 98-99 0.14517504271116977 1107 100-101 -0.003930132152031973 1108 1 0.370622121708152 1108 2 0.250833399854546 1108 3 0.3309277756162068 1108 4 0.2895069199320446 1108 5 0.2886143399519483 1108 6 0.2914639598614883 1108 7 0.35345957516366866 1108 8 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-4.8492024810276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2784.0 3 1010.0 4 999.0 5 1122.0 6 1246.0 7 1370.0 8 1833.0 9 2191.0 10 2799.0 11 3658.0 12 4853.0 13 6172.0 14 7234.0 15 9414.0 16 11923.0 17 15745.0 18 12621.0 19 9010.0 20 8829.0 21 9144.0 22 9984.0 23 11230.0 24 12759.0 25 14841.0 26 17499.0 27 21003.0 28 25674.0 29 31742.0 30 39914.0 31 51066.0 32 66449.0 33 90193.0 34 125449.0 35 177916.0 36 251765.0 37 315037.0 38 221258.0 39 29207.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.083017267345724 17.79876688161203 14.01359749688032 23.10461835416193 2 16.000661425667374 19.402393303618354 39.33546391080835 25.261481359905925 3 20.344617730047396 23.11606720230155 29.186281681655835 27.353033385995218 4 12.896348342064057 15.540709601624462 36.32618584055019 35.23675621576129 5 13.983319448244483 37.14672183811933 33.55766718210576 15.312291531530423 6 33.23621471045819 34.80355923969524 16.630897988549922 15.329328061296652 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28.88775725330942 22.56533249085557 30-31 22.421134090639477 25.545190966662506 28.784835273313103 23.248839669384914 32-33 22.233552428377678 25.706957228061988 28.880900376447638 23.178589967112696 34-35 23.298271795858383 25.61878092060661 28.470222483925433 22.612724799609577 36-37 23.09826085085851 25.259059156886067 28.83831352627163 22.80436646598379 38-39 22.73262575079228 25.656228717820113 28.740101097269243 22.871044434118364 40-41 22.813894695692422 25.749866465714728 28.75845998857967 22.677778850013183 42-43 22.5808801247539 25.925527527179277 28.079277981268476 23.41431436679835 44-45 22.75873917082869 25.755425841632025 28.165756814975108 23.320078172564184 46-47 23.3694534772976 26.228762550851098 27.683033981455274 22.71874999039603 48-49 23.22399224311861 26.213587757403843 27.460066966103597 23.10235303337395 50-51 22.747880786845663 26.50244237356791 27.86112984133638 22.88854699825005 52-53 23.286907530101782 26.561761439107702 27.215459860089485 22.935871170701034 54-55 22.663321185269144 26.298028546823932 27.54566895592828 23.49298131197864 56-57 23.118161431325504 26.499418079160286 27.078788117197966 23.30363237231624 58-59 23.590228394648154 26.010540440365194 27.090646680726326 23.308584484260333 60-61 22.966721279381993 26.248401494734956 27.965183087821977 22.819694138061067 62-63 22.829644249217626 26.371539937410088 27.871657182089436 22.927158631282847 64-65 22.55422514672452 26.767432304809603 27.47710661126216 23.20123593720372 66-67 22.604788578622788 26.604041054795463 27.121559915018345 23.669610451563404 68-69 22.70877823847387 26.39134763137781 27.536843218554214 23.36303091159411 70-71 23.075421209392193 26.317711963708824 27.175611809390166 23.43125501750882 72-73 22.90955698274312 26.240815617714542 27.57999498998766 23.269632409554678 74-75 22.56824457902919 27.194110979846407 27.085410282114587 23.152234159009815 76-77 22.899997202650166 26.51527983181472 27.09245610082018 23.492266864714935 78-79 22.951682642489438 26.677061662145636 27.10431012057662 23.266945574788313 80-81 23.238379241855828 26.718963076962822 26.941385824117493 23.10127185706386 82-83 22.977021103369523 26.658834547455296 27.295898837142445 23.068245512032735 84-85 22.936604102539512 26.916130134859905 26.91665268087623 23.230613081724353 86-87 22.967246513663785 26.96150214307319 26.877064075798152 23.194187267464876 88-89 22.82975990271801 27.03801737538435 26.46151216808685 23.67071055381079 90-91 22.882769327727864 26.80714159448471 26.670734987818097 23.639354089969334 92-93 23.195310144109598 26.835198756650865 26.884535568459977 23.08495553077956 94-95 22.90304555547086 26.521210206274542 26.997001408932086 23.578742829322508 96-97 22.694950222728238 26.430919819063277 27.270869015139127 23.60326094306936 98-99 22.70927420941811 26.85161953676919 26.504387065352148 23.934719188460555 100-101 22.811778356689885 26.411687696224707 26.720050140034747 24.056483807050665 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 175.0 1 146.0 2 210.5 3 333.0 4 292.5 5 235.5 6 479.0 7 1056.0 8 1690.5 9 2051.5 10 1814.0 11 1476.5 12 1497.0 13 1563.0 14 1684.5 15 1822.0 16 1909.5 17 2031.0 18 2202.0 19 2369.5 20 2605.5 21 3078.0 22 3637.5 23 4284.5 24 5176.5 25 6413.0 26 8126.0 27 10184.5 28 12060.5 29 13932.0 30 16597.0 31 19981.5 32 24079.5 33 26374.0 34 27515.5 35 29427.5 36 32056.0 37 35023.5 38 36879.0 39 37908.5 40 39286.5 41 42328.5 42 44650.0 43 46605.0 44 49707.5 45 53688.0 46 55989.0 47 60900.5 48 73642.0 49 73847.0 50 62588.0 51 57632.0 52 56360.0 53 57292.0 54 57846.0 55 55331.0 56 51439.5 57 47333.5 58 44641.5 59 40922.0 60 34685.0 61 29340.0 62 25139.5 63 19918.0 64 14561.5 65 11173.5 66 8651.0 67 7003.0 68 5438.5 69 3972.0 70 3218.0 71 2565.5 72 2046.5 73 1790.0 74 1469.5 75 1142.5 76 854.0 77 542.5 78 349.0 79 248.5 80 172.5 81 112.0 82 70.0 83 41.0 84 24.5 85 20.0 86 17.0 87 15.5 88 13.5 89 8.0 90 4.5 91 5.5 92 3.5 93 1.0 94 1.5 95 3.0 96 3.5 97 2.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011002188759450972 2 0.009834358667665674 3 0.01346077842636739 4 0.019545787851985526 5 0.030978229803146875 6 0.04665173892973904 7 0.005408897267216121 8 0.024647361410837097 9 0.0346661143035215 10-11 0.03961402600819079 12-13 0.029871864453034486 14-15 0.027874260348664897 16-17 0.02778206323615553 18-19 0.023786855027416346 20-21 0.05193770671360934 22-23 0.011739765659525897 24-25 0.0032576313086642543 26-27 9.527034959301121E-4 28-29 1.2292948334582092E-4 30-31 0.0025815191502622393 32-33 0.0018132098793508587 34-35 0.0 36-37 2.4585896669164183E-4 38-39 6.146474167291046E-5 40-41 0.001598083283495672 42-43 0.004056672950412091 44-45 0.0021819983293883214 46-47 0.0010756329792759329 48-49 3.99520820873918E-4 50-51 0.002950307600299702 52-53 0.003196166566991344 54-55 0.0047942498504870154 56-57 5.839150458926493E-4 58-59 0.004425461400449553 60-61 0.0023663925544070525 62-63 5.839150458926493E-4 64-65 3.3805607920100756E-4 66-67 0.015058861709863064 68-69 0.04093551795415837 70-71 0.04723565397563169 72-73 0.01269246915545601 74-75 0.028550372507066907 76-77 0.025169811715056833 78-79 0.05199917145528225 80-81 0.07474112587425912 82-83 0.04545317646711728 84-85 0.018316493018527315 86-87 0.019238464143620973 88-89 0.02074435031460728 90-91 0.034512452449339225 92-93 0.023018545756504968 94-95 0.011370977209488436 96-97 0.018623816726891868 98-99 0.049479117046692916 100-101 0.06776487769438379 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1626949.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.71398962549805 #Duplication Level Percentage of deduplicated Percentage of total 1 79.75613105680046 46.03044520382044 2 11.897690353729635 13.733263552850808 3 3.5591904910706935 6.16245249230476 4 1.5021799104938802 3.4678718307950156 5 0.8383541443746407 2.419238119546565 6 0.4886892283609629 1.6922523033430361 7 0.32515160352801126 1.3136057390910787 8 0.24295720472979412 1.1217623674572277 9 0.19037858036318286 0.9888756670798006 >10 1.0797261493546386 11.534766000349695 >50 0.07393792255542307 2.917816368006293 >100 0.0398587151483709 4.132387991841521 >500 0.00372985892883922 1.500151308291451 >1k 0.0017050783674693577 1.8845531645835905 >5k 3.1970219390050454E-4 1.1005578906386466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6408 0.39386606464001017 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6160 0.37862280870512843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5326 0.32736121414992114 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4631 0.28464321868724834 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3205 0.19699449706167801 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3148 0.19349100678632214 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2877 0.17683406179296338 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1848 0.11358684261153854 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1654 0.1016626827269939 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.146474167291046E-5 0.0 0.0 0.0 0.0 3 6.146474167291046E-5 0.0 0.0 0.0 0.0 4 6.146474167291046E-5 0.0 0.0 0.0 0.0 5 6.146474167291046E-5 0.0 0.0 0.0 0.0 6 6.146474167291046E-5 0.0 0.0 0.0 0.0 7 6.146474167291046E-5 0.0 0.0 0.0 0.0 8 6.146474167291046E-5 0.0 0.0 0.0 0.0 9 6.146474167291046E-5 0.0 0.0 0.0 0.0 10-11 6.146474167291046E-5 0.0 0.0 0.0 0.0 12-13 6.146474167291046E-5 0.0 0.0 0.0 0.0 14-15 6.146474167291046E-5 0.0 0.0 0.0 0.0 16-17 6.146474167291046E-5 0.0 0.0 0.0 0.0 18-19 9.219711250936569E-5 0.0 0.0 0.0 0.0 20-21 1.2292948334582092E-4 0.0 0.0 3.073237083645523E-5 0.0 22-23 1.5366185418227616E-4 0.0 0.0 6.146474167291046E-5 0.0 24-25 1.8439422501873137E-4 0.0 0.0 1.2292948334582092E-4 0.0 26-27 1.8439422501873137E-4 0.0 0.0 3.073237083645523E-4 0.0 28-29 3.380560792010075E-4 0.0 0.0 7.375769000749256E-4 0.0 30-31 3.6878845003746275E-4 0.0 0.0 0.0019361393626966795 0.0 32-33 3.6878845003746275E-4 0.0 0.0 0.005285967783870299 0.0 34-35 3.6878845003746275E-4 0.0 0.0 0.009557767330137575 0.0 36-37 3.6878845003746275E-4 0.0 0.0 0.016165227059975452 0.0 38-39 3.6878845003746275E-4 0.0 0.0 0.02486248800669228 0.0 40-41 3.6878845003746275E-4 0.0 0.0 0.03783154849967639 0.0 42-43 3.6878845003746275E-4 0.0 0.0 0.05129232692604378 0.0 44-45 3.6878845003746275E-4 0.0 0.0 0.06798000429023897 0.0 46-47 3.6878845003746275E-4 0.0 0.0 0.0910292824175804 0.0 48-49 5.531826750561941E-4 0.0 0.0 0.11450881373663219 0.0 50-51 5.531826750561941E-4 0.0 0.0 0.1430591862436991 0.0 52-53 5.531826750561941E-4 0.0 0.0 0.17474426057608444 0.0 54-55 5.531826750561941E-4 0.0 0.0 0.2112235847589568 0.0 56-57 6.146474167291046E-4 0.0 0.0 0.2566152964844012 0.0 58-59 6.146474167291046E-4 0.0 0.0 0.31002815699816033 0.0 60-61 6.146474167291046E-4 0.0 0.0 0.36897284426248145 0.0 62-63 7.375769000749255E-4 0.0 0.0 0.42487502681399353 0.0 64-65 7.375769000749255E-4 0.0 0.0 0.48581731818268425 0.0 66-67 7.375769000749255E-4 0.0 0.0 0.5659980736949959 0.0 68-69 7.375769000749255E-4 0.0 0.0 0.6581337214626888 0.0 70-71 7.375769000749255E-4 0.0 0.0 0.757522208747785 0.0 72-73 7.375769000749255E-4 0.0 0.0 0.8645015916294856 0.0 74-75 7.375769000749255E-4 0.0 0.0 0.9779347723868419 0.0 76-77 7.375769000749255E-4 0.0 0.0 1.1063038853707154 0.0 78-79 7.375769000749255E-4 0.0 0.0 1.2589515713153885 0.0 80-81 8.605063834207465E-4 0.0 0.0 1.429178173378514 0.0 82-83 8.605063834207465E-4 0.0 0.0 1.6041990252921265 0.0 84-85 8.605063834207465E-4 0.0 0.0 1.7866571109481613 0.0 86-87 8.605063834207465E-4 0.0 0.0 1.9891219700187284 0.0 88-89 8.605063834207465E-4 0.0 0.0 2.2142058540249265 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7255 0.0 25.600729 1 TACATGG 7410 0.0 25.065222 2 ACATGGG 7330 0.0 24.237844 3 GAGTACT 6235 0.0 21.565016 12-13 AGTACTT 6395 0.0 21.062616 12-13 GTACTTT 6565 0.0 20.300713 14-15 GTATAGG 405 0.0 19.939163 1 GTGTAGC 835 0.0 19.91107 1 CATGGGG 3620 0.0 19.552578 4 CATGGGA 3420 0.0 19.029213 4 ATGGGTA 660 0.0 18.713533 5 GTATCAA 14140 0.0 18.71194 1 GAGTACA 6880 0.0 18.296602 1 AGAGTAC 10915 0.0 18.259184 10-11 TACTTTT 7300 0.0 18.224194 14-15 ACCGCGC 235 2.525423E-6 18.193981 8 AGTACAT 5775 0.0 18.096046 2 ACTTTTT 7615 0.0 17.844976 16-17 GTATATA 970 0.0 17.629654 1 GGTATCA 11530 0.0 17.262262 1 >>END_MODULE