FastQCFastQC Report
Thu 26 May 2016
SRR936379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936379_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626949
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82750.508620737343334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62290.38286387588055926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60230.3702021390959397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36990.2273580794480958No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29370.18052194629333804No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21430.1317189414050471No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20680.12710908577957883No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC20390.12532660827106443No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19540.12010210522886704No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18730.11512346115336129No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.1134024483865198No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16580.10190854169368553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT6000.022.95714
GTAAGCG2200.022.66923794-95
TAAGGTG7400.022.465015
GTACCTG13500.021.819011
TACCTGG12650.021.7794932
GAGTACT76900.021.71147312-13
GTACATG72550.021.5444561
AGTACTT77800.021.27715112-13
GTACTTT80150.020.74251714-15
TACATGG72700.020.5819472
ACATGGG73500.019.4513363
ATTCGCG2700.019.35081380-81
AGAGTAC124150.018.82357210-11
TACTTTT88100.018.68204114-15
CAATTCG2550.018.62816878-79
ACTTTTT91750.018.2491816-17
CGCGGAA2350.018.19051282-83
GTGTAAG6900.017.901991
GGTAAGG7500.017.7323823
CATGGGG38300.017.6100224
Produced by FastQC (version 0.11.5)