FastQCFastQC Report
Thu 26 May 2016
SRR936378_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936378_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618225
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64470.3983994809127285No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59270.36626550695978616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54380.33604721222326933No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47250.291986590245485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.20145529824344574No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30900.19094996060498387No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29210.18050641907027762No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17870.1104296374113612No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16960.10480619196959631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG75650.026.445061
TACATGG75900.025.6050742
ACATGGG75400.024.325433
GAGTACT60700.023.01245112-13
GTACTTT64500.021.47185914-15
GTGTAAG7550.021.3994871
CATGGGG36200.021.2642944
GTATAGG3850.020.9826151
GTAAGGT7650.020.4973184
TAAGGTG8450.020.2449725
AGTACTT63250.020.2067312-13
GTGTAGC9750.019.9825381
GGTAAGG7200.019.7985483
AGGTAAG7000.019.6854112
GTATCAA140400.019.5290051
GGTATCA111900.019.4494251
GTACCTG12800.018.9335291
GTACACG2552.6650014E-718.6350771
GTATAGC4900.018.4259071
AGAGTAC106750.018.4042710-11
Produced by FastQC (version 0.11.5)