FastQCFastQC Report
Thu 26 May 2016
SRR936378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936378_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618225
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81680.5047505754762163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63800.3942591419610993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63620.3931468120934975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38220.23618470855412566No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26910.16629331520647625No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20430.1262494399728097No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20170.12464274127516262No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19700.12173832439864667No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19140.1182777425883298No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17980.11110939455267345No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17940.11086221013765082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG8800.025.3632895
GTAAGGT8400.024.3142034
TAACGCG853.1508134E-622.35384476-77
GAGTACT76050.021.3870912-13
GTACATG69600.021.1672971
TACATGG69900.020.8653662
ATACCGT1605.0000504E-520.7763086
GTATAGG3700.020.7517411
TATAGGG3252.1827873E-1120.4648932
ACATGGG69450.020.3146213
GTACTTT79850.020.2503514-15
GGTAAGG9750.019.4885673
AGTACTT78900.019.23021912-13
ACTTTTT92400.017.73117416-17
AGGTAAG9650.017.7231122
CTAATAG3301.20999175E-817.2739583
CATGGGA33050.017.2456934
TACTTTT86800.017.2064514-15
AGAGTAC124000.017.09976410-11
AAGGTGT14100.016.8399686
Produced by FastQC (version 0.11.5)