FastQCFastQC Report
Thu 26 May 2016
SRR936377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652148
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64680.39149035074339583No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59640.3609846091270274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55450.33562368504516543No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47860.28968349082527717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.19695572067393477No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31870.19290039391144134No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28280.17117110573628996No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18200.11015962250355295No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16990.10283582342502004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG72550.025.3474251
TACATGG72050.024.7319032
ACCGTGC2950.024.1610818
ACATGGG74000.022.9243373
GAGTACT63700.021.41093412-13
TGTACGC1150.003715685620.66012
GTAAGGT7200.020.4592384
AGTACTT64900.020.3926512-13
GTACTTT66850.020.2957214-15
ATGGGCG3850.019.7472385
TAAGGTG7450.019.134285
ACCGTCC2251.6628965E-619.0067188
GTACCTG13850.018.5269661
AGAGTAC110150.018.42003610-11
GTATCAA147600.018.3505381
CATGGGG37300.018.3448414
AGTACAT56150.017.940992
GGTAAGG8250.017.8553353
CATGGGA36300.017.8029754
TACCTGG13150.017.7064132
Produced by FastQC (version 0.11.5)