##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936377_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1652148 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79045279236485 33.0 31.0 34.0 30.0 34.0 2 31.86729155015168 33.0 31.0 34.0 30.0 34.0 3 31.926267501458707 33.0 31.0 34.0 30.0 34.0 4 35.555073153252614 37.0 35.0 37.0 33.0 37.0 5 35.53549621462484 37.0 35.0 37.0 33.0 37.0 6 35.671264317724564 37.0 35.0 37.0 33.0 37.0 7 35.561249355384625 37.0 35.0 37.0 33.0 37.0 8 35.51829436588005 37.0 35.0 37.0 33.0 37.0 9 37.08604979699155 39.0 37.0 39.0 33.0 39.0 10-11 37.00500651273373 39.0 37.0 39.0 33.0 39.0 12-13 36.91162050857429 39.0 37.0 39.0 33.0 39.0 14-15 38.04764706309604 40.0 38.0 41.0 33.0 41.0 16-17 37.931946472107825 40.0 38.0 41.0 33.0 41.0 18-19 37.88102458133291 40.0 38.0 41.0 33.0 41.0 20-21 37.78234365202149 40.0 37.5 41.0 32.5 41.0 22-23 37.65028950190903 40.0 37.0 41.0 32.0 41.0 24-25 37.78856040742113 40.0 37.0 41.0 33.0 41.0 26-27 37.76500168265797 40.0 37.0 41.0 33.0 41.0 28-29 37.66772165689757 40.0 37.0 41.0 33.0 41.0 30-31 37.48696272973123 40.0 37.0 41.0 32.0 41.0 32-33 37.29614326319434 39.0 36.5 41.0 32.0 41.0 34-35 37.07218239528178 39.0 36.0 41.0 31.0 41.0 36-37 36.8839171793326 39.0 36.0 41.0 31.0 41.0 38-39 36.67310374131131 39.0 35.5 40.5 30.5 41.0 40-41 36.487807085079545 39.0 35.0 40.0 30.0 41.0 42-43 36.239520309318536 39.0 35.0 40.0 30.0 41.0 44-45 35.96956114101158 38.5 35.0 40.0 29.5 41.0 46-47 35.669980231795215 38.5 35.0 40.0 28.0 41.0 48-49 35.54495390243489 39.0 35.0 40.0 28.0 41.0 50-51 34.61474002329089 38.0 34.0 39.5 26.0 40.5 52-53 34.751126109767405 38.0 34.0 39.5 26.5 40.5 54-55 35.15528602764401 38.0 35.0 40.0 27.0 41.0 56-57 35.03754475991255 38.0 34.5 40.0 26.5 41.0 58-59 34.76859669956929 38.0 34.0 40.0 26.0 41.0 60-61 34.425987260221234 37.5 34.0 40.0 26.0 41.0 62-63 34.099579456562005 37.0 34.0 40.0 25.5 41.0 64-65 33.746142597394424 36.5 33.0 39.5 24.5 41.0 66-67 33.34787258768585 36.0 33.0 39.0 23.5 41.0 68-69 32.93493440055008 35.0 32.5 39.0 22.5 40.0 70-71 32.49109764984735 35.0 32.0 38.0 21.5 40.0 72-73 32.10079000186424 35.0 32.0 37.0 20.5 39.5 74-75 31.689161019472834 35.0 31.0 37.0 20.0 39.0 76-77 31.272669276602336 35.0 31.0 36.0 18.5 39.0 78-79 30.8367667424468 34.5 31.0 36.0 17.5 38.5 80-81 30.381833225594804 34.0 30.0 35.0 12.0 37.0 82-83 29.908484288332524 34.0 30.0 35.0 7.0 37.0 84-85 29.60888279984602 34.0 29.5 35.0 7.0 36.0 86-87 29.322605480864908 34.0 29.0 35.0 2.0 36.0 88-89 28.935219483968748 34.0 29.0 35.0 2.0 35.5 90-91 28.53552829407535 34.0 29.0 35.0 2.0 35.0 92-93 28.174714674472263 34.0 28.5 35.0 2.0 35.0 94-95 27.717076799415068 34.0 27.0 35.0 2.0 35.0 96-97 27.13816770652508 33.0 26.5 35.0 2.0 35.0 98-99 26.44985648985442 33.0 25.0 35.0 2.0 35.0 100-101 23.874151710379458 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2566482667279075 1101 2 0.3106160520153267 1101 3 0.38467550915637005 1101 4 0.3774274624906724 1101 5 0.3699372410664097 1101 6 0.2271343519584761 1101 7 0.38110879302001166 1101 8 0.3669184152671292 1101 9 0.6680235802896348 1101 10-11 0.49636812720392953 1101 12-13 0.5619333594060336 1101 14-15 0.7460953495058931 1101 16-17 0.8055621511091218 1101 18-19 0.8506246733742415 1101 20-21 0.792826655135876 1101 22-23 0.7238519653029414 1101 24-25 0.7077519715622813 1101 26-27 0.6256975117718397 1101 28-29 0.6162783772216258 1101 30-31 0.5790790145026605 1101 32-33 0.521877460215606 1101 34-35 0.5247294056444787 1101 36-37 0.6458835117364146 1101 38-39 0.5197581431232052 1101 40-41 0.42098952495308595 1101 42-43 0.4175958844578247 1101 44-45 0.3295663235208224 1101 46-47 0.44109072580780406 1101 48-49 0.43962086865006 1101 50-51 0.3296990474647927 1101 52-53 0.29167342623038195 1101 54-55 0.31288316483465195 1101 56-57 0.3035035127499768 1101 58-59 0.29911529066217923 1101 60-61 0.22518513420055086 1101 62-63 0.13530589796474857 1101 64-65 0.08799154925705466 1101 66-67 0.21791007615905045 1101 68-69 0.12097473398417691 1101 70-71 0.05588945162423187 1101 72-73 0.05547293345376403 1101 74-75 0.2069838454713313 1101 76-77 0.15111274500218386 1101 78-79 0.26574936565702956 1101 80-81 0.35762753756378984 1101 82-83 0.4931460766883262 1101 84-85 0.4489644033590743 1101 86-87 0.3571086173529672 1101 88-89 0.2551526684041079 1101 90-91 0.20412531320143046 1101 92-93 0.3394098637188385 1101 94-95 0.4550936463655333 1101 96-97 0.10739060369696318 1101 98-99 0.2623435367105529 1101 100-101 0.6296925413630845 1102 1 0.11567230568404696 1102 2 0.298765892555771 1102 3 0.19559591654980935 1102 4 0.14858371570558404 1102 5 0.23784943543559933 1102 6 0.14153629290700565 1102 7 0.13163175176628528 1102 8 0.06762526688401493 1102 9 0.17054621325832642 1102 10-11 0.18269254166158788 1102 12-13 0.2068100783114204 1102 14-15 0.23471242368030687 1102 16-17 0.10426589926590424 1102 18-19 0.36108706320998607 1102 20-21 0.30634642469110673 1102 22-23 0.22961308460611463 1102 24-25 0.06684888181913351 1102 26-27 -0.017348851521596487 1102 28-29 0.04283696574739082 1102 30-31 -0.15493966626959832 1102 32-33 -0.1319002737441295 1102 34-35 -0.37573589904329197 1102 36-37 -0.21969680101005906 1102 38-39 -0.2695784279820401 1102 40-41 -0.2150944142122384 1102 42-43 -0.1593165585310956 1102 44-45 -0.51135669866634 1102 46-47 -0.31672257791978353 1102 48-49 -0.3005865144986686 1102 50-51 -0.3406011822941295 1102 52-53 -0.2576057908746563 1102 54-55 -0.19921239944641655 1102 56-57 -0.1331381061931367 1102 58-59 0.011089331332932773 1102 60-61 -0.10843146845368068 1102 62-63 -0.11003355370014845 1102 64-65 0.005760572953086296 1102 66-67 0.024713255062387418 1102 68-69 -0.11043022469350916 1102 70-71 -0.1938437108187543 1102 72-73 -0.16325349053718696 1102 74-75 -0.2048793879568791 1102 76-77 -0.08057512578414006 1102 78-79 -0.1929443948450107 1102 80-81 -0.15427084555064496 1102 82-83 -0.1075588459823642 1102 84-85 -0.11600357613408718 1102 86-87 -0.33385705597785176 1102 88-89 -0.2679250295486355 1102 90-91 -0.17608069825222472 1102 92-93 -0.3569932946090617 1102 94-95 -0.3997831582707967 1102 96-97 -0.3042936663897926 1102 98-99 -0.26739545543649257 1102 100-101 -0.18584126234734555 1103 1 0.2555336502037129 1103 2 0.3674723399600097 1103 3 0.2805766702446242 1103 4 0.312713819432588 1103 5 0.3556161404076761 1103 6 0.28337897002269585 1103 7 0.319478714478997 1103 8 0.30212966912530703 1103 9 0.464465212450456 1103 10-11 0.46168039877667155 1103 12-13 0.3738309079546269 1103 14-15 0.4044239019088707 1103 16-17 0.6141182902575153 1103 18-19 0.538201267674431 1103 20-21 0.5454002236223019 1103 22-23 0.5779552778640777 1103 24-25 0.5693127220942387 1103 26-27 0.5517669308806674 1103 28-29 0.1979066174994344 1103 30-31 0.22236851330209362 1103 32-33 0.43831795905191484 1103 34-35 0.35408855672476847 1103 36-37 0.3495199650922558 1103 38-39 0.45005963455657394 1103 40-41 0.24851027726365515 1103 42-43 0.1607700444420388 1103 44-45 0.22622109118388067 1103 46-47 0.33506604861549505 1103 48-49 0.39892055544036964 1103 50-51 0.47737407831942136 1103 52-53 0.3417628449921679 1103 54-55 0.4118935664761736 1103 56-57 0.4187068984078337 1103 58-59 0.2527074142194934 1103 60-61 0.23572003625745452 1103 62-63 0.3265080461840739 1103 64-65 0.43227981061794907 1103 66-67 0.37266933417312487 1103 68-69 0.39253772405213994 1103 70-71 0.49497407246482084 1103 72-73 0.3768474337606804 1103 74-75 0.3203534415739959 1103 76-77 0.4052760858042497 1103 78-79 0.4779792927989064 1103 80-81 0.41271307374016075 1103 82-83 0.3637989095658014 1103 84-85 0.25138011980453356 1103 86-87 0.4142272238301601 1103 88-89 0.5992245678126125 1103 90-91 0.554862543508154 1103 92-93 0.6258740376499894 1103 94-95 0.5951630665588787 1103 96-97 0.6167222296326358 1103 98-99 0.706156940523293 1103 100-101 1.114180365445094 1104 1 0.08068879122665962 1104 2 0.1409089588666994 1104 3 0.09906764167507731 1104 4 0.3103641000790276 1104 5 0.3386121629252443 1104 6 0.1538082609771152 1104 7 0.176431213816997 1104 8 0.2201943543224587 1104 9 0.37810705792546884 1104 10-11 0.2960627776651492 1104 12-13 0.15021613241003706 1104 14-15 0.32190630233803574 1104 16-17 0.1871776040011639 1104 18-19 0.40552170128724896 1104 20-21 0.47287860403645965 1104 22-23 0.30140897840451686 1104 24-25 0.42111370253885383 1104 26-27 0.024652135441805 1104 28-29 0.32301716791220514 1104 30-31 0.2880937006855646 1104 32-33 0.05592699681398727 1104 34-35 -0.08452787210244139 1104 36-37 0.10090714467441586 1104 38-39 0.19368063906640032 1104 40-41 0.2674492128240473 1104 42-43 0.1063594842879283 1104 44-45 0.09771726393750413 1104 46-47 0.15542214236828755 1104 48-49 0.171999763456256 1104 50-51 0.12429410197302815 1104 52-53 0.11436404166693848 1104 54-55 0.333000703306233 1104 56-57 0.09968317208716115 1104 58-59 0.1475877261616816 1104 60-61 0.25827437849107326 1104 62-63 0.38661660030953016 1104 64-65 0.29655985062602497 1104 66-67 0.4869827828383606 1104 68-69 0.2650692221731532 1104 70-71 0.31570692257314903 1104 72-73 0.15428856386510148 1104 74-75 0.018922563193857656 1104 76-77 0.1499380409584461 1104 78-79 0.12035669949133876 1104 80-81 0.18199178975011066 1104 82-83 0.03462828119491945 1104 84-85 0.1630322636524717 1104 86-87 0.3925298445232208 1104 88-89 0.3516817848371687 1104 90-91 0.27107500335945645 1104 92-93 0.22641378983596283 1104 94-95 0.30732162253655915 1104 96-97 0.4684783450633212 1104 98-99 0.5668525814824434 1104 100-101 1.0014503636477485 1105 1 0.349431024558001 1105 2 0.39926966215365667 1105 3 0.32893089143408005 1105 4 0.2984325064794717 1105 5 0.29404364135772454 1105 6 0.3381082868541583 1105 7 0.4155738020335562 1105 8 0.4551758564992596 1105 9 0.49383120291656013 1105 10-11 0.4777214963816405 1105 12-13 0.3560511221587461 1105 14-15 0.295801622573606 1105 16-17 0.4655826395039 1105 18-19 0.23429711979871826 1105 20-21 0.21276906734934897 1105 22-23 0.19733590276106838 1105 24-25 0.2617638420189863 1105 26-27 0.30608052000315666 1105 28-29 0.2997812858319193 1105 30-31 0.3210165558053788 1105 32-33 0.43151470436354344 1105 34-35 0.3113401890811289 1105 36-37 0.20795053564816612 1105 38-39 0.06734477205736056 1105 40-41 0.040883722567755854 1105 42-43 0.33241198341351463 1105 44-45 0.26064782529881114 1105 46-47 0.34679072535824673 1105 48-49 0.4029471477417772 1105 50-51 0.5405943667151192 1105 52-53 0.523267025489524 1105 54-55 0.48353559039984617 1105 56-57 0.5957387707711277 1105 58-59 0.5332497093671904 1105 60-61 0.3785100871098379 1105 62-63 0.558730101019016 1105 64-65 0.6213293031985145 1105 66-67 0.6213757954669248 1105 68-69 0.5313214295421389 1105 70-71 0.7099143029344575 1105 72-73 0.6466333655644618 1105 74-75 0.8453310849888744 1105 76-77 0.6577428142783575 1105 78-79 0.7272574823431022 1105 80-81 0.4837036559401824 1105 82-83 0.561341355928402 1105 84-85 0.6685470604892139 1105 86-87 0.7256881523349605 1105 88-89 0.7795724073934238 1105 90-91 0.6614292629773253 1105 92-93 0.65972366320905 1105 94-95 0.6688939232904083 1105 96-97 0.7455745991474849 1105 98-99 0.9627853352836695 1105 100-101 1.7846642644561967 1106 1 0.041443347745083514 1106 2 0.1319836080551049 1106 3 0.19277722685960796 1106 4 0.1598105729012076 1106 5 0.07773297114823663 1106 6 0.046063279379140454 1106 7 0.24230168112590178 1106 8 0.19649605962605676 1106 9 0.3502884682100813 1106 10-11 0.26145743154570056 1106 12-13 0.3161635803198237 1106 14-15 0.47683232764632777 1106 16-17 0.5457099871739359 1106 18-19 0.49781630971259716 1106 20-21 0.4545550215792389 1106 22-23 0.33473278080630564 1106 24-25 0.21234671182051557 1106 26-27 0.12935748302754746 1106 28-29 0.3813757948715377 1106 30-31 0.30563647990519627 1106 32-33 0.24181388665108017 1106 34-35 0.29131561533872485 1106 36-37 0.16986123491034277 1106 38-39 0.21217944403421285 1106 40-41 0.23525445923528565 1106 42-43 0.061488591752848265 1106 44-45 0.29742400450555095 1106 46-47 0.2493634025040663 1106 48-49 0.3798950405131052 1106 50-51 0.10775573630783697 1106 52-53 0.39913361306827966 1106 54-55 0.48743258647167664 1106 56-57 0.39922055533325107 1106 58-59 0.2857297810305184 1106 60-61 0.32306785053611264 1106 62-63 0.5771949079093446 1106 64-65 0.3658885797862723 1106 66-67 0.4746145662955925 1106 68-69 0.37488534850931643 1106 70-71 0.5649473806443766 1106 72-73 0.5149492773653037 1106 74-75 0.2840562161799731 1106 76-77 0.36567874096870767 1106 78-79 0.5370688213699566 1106 80-81 0.35678027638480714 1106 82-83 0.6348452931745676 1106 84-85 0.6555541481375791 1106 86-87 0.7120609226915207 1106 88-89 0.8117603783649336 1106 90-91 0.7975526643222253 1106 92-93 0.9005578388689379 1106 94-95 0.9084264774464579 1106 96-97 0.9823810415837961 1106 98-99 1.107057716455099 1106 100-101 1.4739338311472743 1107 1 0.20730646204152592 1107 2 0.21828683603916943 1107 3 0.029164521857541814 1107 4 0.1962808308434134 1107 5 0.2603489221149289 1107 6 0.2654390699718405 1107 7 0.2925321162239598 1107 8 0.2987190249046492 1107 9 0.409641357244773 1107 10-11 0.4073168550951962 1107 12-13 0.4344581713251259 1107 14-15 0.3910249846371414 1107 16-17 0.4158034230069916 1107 18-19 0.5482133015960997 1107 20-21 0.5077221240905274 1107 22-23 0.45143498799916415 1107 24-25 0.5649143045387106 1107 26-27 0.3988996726814733 1107 28-29 0.36417249780426886 1107 30-31 0.5616768446897993 1107 32-33 0.41766725359018864 1107 34-35 0.5159229608714995 1107 36-37 0.5636465848530889 1107 38-39 0.5831001434465364 1107 40-41 0.5127798607223113 1107 42-43 0.5489969905680212 1107 44-45 0.4358787486926232 1107 46-47 0.4421049335016036 1107 48-49 0.4085659930728056 1107 50-51 0.4244615711579556 1107 52-53 0.4871150268451814 1107 54-55 0.5678571170055093 1107 56-57 0.6724400853781347 1107 58-59 0.6668853633641447 1107 60-61 0.4924376476081278 1107 62-63 0.675390385009365 1107 64-65 0.7269857410656115 1107 66-67 0.7512641251409207 1107 68-69 0.686756778027096 1107 70-71 0.6787998264739912 1107 72-73 0.7388321955158901 1107 74-75 0.534352106832916 1107 76-77 0.48415140262815726 1107 78-79 0.5522672857668667 1107 80-81 0.792201037309578 1107 82-83 0.9977665351582345 1107 84-85 1.0051224569237682 1107 86-87 0.9218953437962867 1107 88-89 0.746189684788412 1107 90-91 0.8836762444098554 1107 92-93 1.0883252208675565 1107 94-95 1.1487352699413087 1107 96-97 1.1739319123792633 1107 98-99 1.4416720333987811 1107 100-101 1.8189663065659545 1108 1 0.09167816524544037 1108 2 0.22290055749193272 1108 3 0.09912310529639612 1108 4 0.1733377381895025 1108 5 0.28315248164406626 1108 6 0.18012923471220432 1108 7 0.047503146799968476 1108 8 0.17960468207306235 1108 9 0.26753078180755807 1108 10-11 0.07475171464271568 1108 12-13 0.2385831682280184 1108 14-15 0.2594700248865749 1108 16-17 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-2.4359712036944714 2316 54-55 -2.530392459504931 2316 56-57 -1.5939146778710338 2316 58-59 -1.9752054650620892 2316 60-61 -1.5349883425507826 2316 62-63 -1.2702819550729103 2316 64-65 -1.5986172273945058 2316 66-67 -2.0949486682768423 2316 68-69 -2.457995984738094 2316 70-71 -2.3714945995968293 2316 72-73 -1.8056140414769288 2316 74-75 -1.4766182892306468 2316 76-77 -1.5670303348889867 2316 78-79 -2.394252033423257 2316 80-81 -1.9740327289241897 2316 82-83 -1.6675960525646012 2316 84-85 -1.5995399614032486 2316 86-87 -2.3207036720946554 2316 88-89 -2.793658665618942 2316 90-91 -2.284711268698153 2316 92-93 -2.8601300505690794 2316 94-95 -3.266486154596887 2316 96-97 -4.085364691637839 2316 98-99 -5.56897570395703 2316 100-101 -6.038660538638531 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2969.0 3 1102.0 4 1117.0 5 1139.0 6 1330.0 7 1429.0 8 1820.0 9 2223.0 10 2765.0 11 3623.0 12 4809.0 13 6271.0 14 7313.0 15 9388.0 16 11754.0 17 15568.0 18 13225.0 19 8927.0 20 8680.0 21 9060.0 22 10081.0 23 11173.0 24 12755.0 25 14765.0 26 17735.0 27 21206.0 28 25741.0 29 32409.0 30 40677.0 31 52220.0 32 68351.0 33 91495.0 34 127265.0 35 181373.0 36 254883.0 37 318094.0 38 225685.0 39 31712.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.025202604723255 17.772180562661394 13.99496856107507 23.20764827154028 2 16.015273109447197 19.364665769055666 39.30615961331693 25.31390150818021 3 20.34788441548771 23.12773903583517 29.148517416923102 27.375859131754016 4 12.962996605226474 15.569753936447611 36.28017357793143 35.18707588039449 5 14.018363891371804 37.03742057695708 33.6248640451915 15.319351486479613 6 33.253724257494994 34.75802090707114 16.64160716675457 15.346647668679298 7 29.49145255151783 32.33218479202117 19.657435947122693 18.518926709338306 8 27.306713461255498 33.22058550154922 19.077332781099056 20.395368256096226 9 26.485628284567774 16.621282939533373 20.157883295700262 36.73520548019859 10-11 25.91354893718034 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22.67922451959221 36-37 23.068273991840293 25.22980956647014 28.818295178024478 22.883621263665088 38-39 22.714258128524914 25.643057377431234 28.753546478158782 22.889138015885074 40-41 22.814997161233467 25.76062600552258 28.765784207742023 22.658592625501928 42-43 22.58999773019596 25.897162744949686 28.108526897177878 23.404312627676475 44-45 22.72561232670377 25.784620431742116 28.197852396359707 23.291914845194402 46-47 23.355418757004152 26.211008655109367 27.68751299446689 22.746059593419588 48-49 23.23654641919287 26.15483182513288 27.46244328170618 23.14617847396807 50-51 22.7595874576422 26.52266401476141 27.824577193715687 22.8931713338807 52-53 23.312531588488625 26.506456914058123 27.200208219211248 22.980803278242 54-55 22.63955417962653 26.331893813494766 27.542385833889966 23.48616617298874 56-57 23.113565908131967 26.470643429769 27.126337767182658 23.28945289491638 58-59 23.527980351178794 26.01577172016471 27.180778537627702 23.275469391028793 60-61 22.92910426868338 26.229141526631565 27.955779567329593 22.885974637355467 62-63 22.816951878439042 26.428860891127016 27.843565355478393 22.91062187495555 64-65 22.548464181175053 26.783345075622766 27.462763626503197 23.20542711669899 66-67 22.613311251723992 26.601680877768185 27.14745618104983 23.637551689458 68-69 22.704541216980285 26.350633092236052 27.557279773738237 23.387545917045426 70-71 23.023483579169614 26.404529408346487 27.131959846203014 23.44002716628088 72-73 22.927729519712027 26.32374419909534 27.52644643556464 23.222079845627995 74-75 22.601834275974277 27.183741138529484 27.074607044626948 23.139817540869295 76-77 22.929029497068935 26.549722752793432 27.054944915258083 23.46630283487955 78-79 22.985845174923423 26.693780429968438 27.077757563853183 23.24261683125496 80-81 23.254273156980197 26.765021993444794 26.865054106628484 23.115650742946524 82-83 23.079679539775004 26.778966763646512 27.168389312383347 22.97296438419514 84-85 22.943061106814717 26.965604810211257 26.900467793952732 23.190866289021294 86-87 22.902241712744804 27.02006858000648 26.8662931991998 23.21139650804892 88-89 22.79926116686122 27.037624741649008 26.437133659127714 23.725980432362054 90-91 22.84937092109548 26.863539572107708 26.65643926692 23.63065023987681 92-93 23.195857097843646 26.886246050346706 26.851074121057422 23.06682273075222 94-95 22.883258625229647 26.646942920183903 26.954663631933705 23.515134822652747 96-97 22.68904638129978 26.50281295058965 27.223393192017927 23.584747476092645 98-99 22.722964847286548 26.88303405514653 26.504636811595343 23.889364285971578 100-101 22.75412029692779 26.468290969859165 26.777721938991757 23.99986679422129 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 168.0 1 160.5 2 237.0 3 337.0 4 285.5 5 270.5 6 518.5 7 1079.5 8 1777.0 9 2087.5 10 1807.5 11 1510.5 12 1516.0 13 1580.0 14 1727.0 15 1868.0 16 1959.5 17 2115.0 18 2203.0 19 2354.5 20 2685.0 21 3156.5 22 3665.5 23 4326.5 24 5334.0 25 6611.5 26 8246.0 27 10326.0 28 12138.0 29 14170.5 30 17050.0 31 20368.5 32 24388.0 33 26586.0 34 27903.0 35 29959.0 36 32749.5 37 35723.0 38 37362.5 39 38545.5 40 39988.0 41 42823.5 42 45016.5 43 47138.5 44 50429.5 45 54481.0 46 56837.0 47 61922.0 48 74415.5 49 74158.0 50 63074.0 51 58340.0 52 57172.0 53 58098.5 54 58492.5 55 56024.0 56 52351.0 57 48271.0 58 45916.0 59 42120.0 60 35458.5 61 30169.0 62 25665.5 63 20254.0 64 14949.5 65 11458.0 66 8889.5 67 7093.0 68 5523.0 69 4131.5 70 3278.0 71 2512.0 72 1968.0 73 1751.0 74 1421.0 75 1138.5 76 899.5 77 579.5 78 363.5 79 252.5 80 167.0 81 121.5 82 84.5 83 40.5 84 30.0 85 22.0 86 19.5 87 15.5 88 11.0 89 7.5 90 8.0 91 9.0 92 4.5 93 2.0 94 2.5 95 3.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032261032304611935 2 0.021547706379815852 3 0.020276633812467162 4 0.04757443037790803 5 0.05229555705663173 6 0.0450928125083225 7 0.0470902122570133 8 0.040855903950493544 9 0.040855903950493544 10-11 0.04757443037790803 12-13 0.04073484942026986 14-15 0.036043986374102076 16-17 0.04270198553640473 18-19 0.04639414870822711 20-21 0.010259371436457266 22-23 0.01728053418943097 24-25 0.004690863046167777 26-27 0.009411989724891474 28-29 0.004932972106615145 30-31 0.007565908138980284 32-33 0.0029355723579243503 34-35 0.00690010822275002 36-37 0.007747489934315812 38-39 0.00950278062255924 40-41 0.0013315998324605301 42-43 0.0013921270975723724 44-45 0.0016644997905756625 46-47 1.513181627796057E-4 48-49 8.171180790098707E-4 50-51 1.513181627796057E-4 52-53 0.002602672399809218 54-55 0.0055987720228454115 56-57 0.014223907301282935 58-59 0.00850408074821384 60-61 0.009169880664444106 62-63 0.004327699455496723 64-65 0.0 66-67 0.005719826553069096 68-69 0.015494979868631624 70-71 0.006052726511184228 72-73 0.00211845427891448 74-75 0.0031474177858157987 76-77 0.006204044673963833 78-79 0.00584088108329278 80-81 0.010804116822463848 82-83 0.005235608432174357 84-85 0.014859443584957279 86-87 0.0038132177020460635 88-89 0.0050540266368388305 90-91 0.003026363255592114 92-93 0.015888407091858597 94-95 0.00986594421323029 96-97 0.008836980706328972 98-99 0.01891477034745071 100-101 0.034379486583526415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1652148.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.62651415207733 #Duplication Level Percentage of deduplicated Percentage of total 1 79.64277734564014 45.89535635819274 2 11.953791066442331 13.77710620122629 3 3.5795387500880347 6.188290213195719 4 1.5156627382748549 3.4936944094788878 5 0.8159038523078264 2.350884744587568 6 0.5013506119315447 1.7334652880175474 7 0.3487567593835484 1.4068345431174074 8 0.2608400644553116 1.2025042932610222 9 0.1904641429945645 0.9878206168565594 >10 1.0672224285650673 11.32434033291709 >50 0.07923106351728168 3.104048673415821 >100 0.03878561985132168 4.078821550369598 >500 0.0036786014663911385 1.4984915362543305 >1k 0.0016816463846359491 1.8695258174512923 >5k 3.153086971192404E-4 1.0888154216581423 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6468 0.39149035074339583 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5964 0.3609846091270274 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5545 0.33562368504516543 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4786 0.28968349082527717 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3254 0.19695572067393477 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3187 0.19290039391144134 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2828 0.17117110573628996 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1820 0.11015962250355295 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1699 0.10283582342502004 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.026363255592114E-5 0.0 24-25 6.052726511184228E-5 0.0 0.0 9.079089766776342E-5 3.026363255592114E-5 26-27 6.052726511184228E-5 0.0 0.0 2.4210906044736912E-4 6.052726511184228E-5 28-29 9.079089766776342E-5 0.0 0.0 7.263271813421074E-4 6.052726511184228E-5 30-31 1.2105453022368456E-4 0.0 0.0 0.001967136116134874 6.052726511184228E-5 32-33 1.2105453022368456E-4 0.0 0.0 0.00475139031127962 6.052726511184228E-5 34-35 2.1184542789144797E-4 0.0 0.0 0.009260671562111869 6.052726511184228E-5 36-37 2.723726930032903E-4 0.0 0.0 0.015283134440740177 6.052726511184228E-5 38-39 3.631635906710537E-4 0.0 0.0 0.023424051598282963 6.052726511184228E-5 40-41 3.631635906710537E-4 0.0 0.0 0.035317659192759976 6.052726511184228E-5 42-43 4.539544883388171E-4 0.0 0.0 0.0483915484569179 6.052726511184228E-5 44-45 4.8421812089473825E-4 0.0 0.0 0.06479443730222717 6.052726511184228E-5 46-47 4.8421812089473825E-4 0.0 0.0 0.08449606209613182 6.052726511184228E-5 48-49 4.8421812089473825E-4 0.0 0.0 0.10486348680626675 6.052726511184228E-5 50-51 4.8421812089473825E-4 0.0 0.0 0.12992177456256945 6.052726511184228E-5 52-53 4.8421812089473825E-4 0.0 0.0 0.15900512544880968 1.2105453022368456E-4 54-55 4.8421812089473825E-4 0.0 0.0 0.19232538489287884 1.2105453022368456E-4 56-57 4.8421812089473825E-4 0.0 0.0 0.23336287063870792 1.2105453022368456E-4 58-59 4.8421812089473825E-4 0.0 0.0 0.28178468272818175 1.2105453022368456E-4 60-61 4.8421812089473825E-4 0.0 0.0 0.3369855485101819 1.2105453022368456E-4 62-63 4.8421812089473825E-4 0.0 0.0 0.3899771691155998 1.2105453022368456E-4 64-65 4.8421812089473825E-4 0.0 0.0 0.4469333255858434 1.2105453022368456E-4 66-67 4.8421812089473825E-4 0.0 0.0 0.5218660797943042 1.2105453022368456E-4 68-69 4.8421812089473825E-4 0.0 0.0 0.6111135322017156 1.2105453022368456E-4 70-71 4.8421812089473825E-4 0.0 0.0 0.7037807750879461 1.2105453022368456E-4 72-73 5.447453860065806E-4 0.0 0.0 0.8038020806852655 1.2105453022368456E-4 74-75 6.052726511184228E-4 0.0 0.0 0.9129327396819171 1.2105453022368456E-4 76-77 6.052726511184228E-4 0.0 0.0 1.030355633998891 1.2105453022368456E-4 78-79 6.052726511184228E-4 0.0 0.0 1.1705670436304736 1.2105453022368456E-4 80-81 6.052726511184228E-4 0.0 0.0 1.3315393051954183 1.2105453022368456E-4 82-83 6.657999162302651E-4 0.0 0.0 1.49750506613209 1.2105453022368456E-4 84-85 6.657999162302651E-4 0.0 0.0 1.6678590537893698 1.2105453022368456E-4 86-87 6.657999162302651E-4 0.0 0.0 1.8591857388079034 1.2105453022368456E-4 88-89 6.657999162302651E-4 0.0 0.0 2.0773865295360947 1.2105453022368456E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7255 0.0 25.347425 1 TACATGG 7205 0.0 24.731903 2 ACCGTGC 295 0.0 24.161081 8 ACATGGG 7400 0.0 22.924337 3 GAGTACT 6370 0.0 21.410934 12-13 TGTACGC 115 0.0037156856 20.6601 2 GTAAGGT 720 0.0 20.459238 4 AGTACTT 6490 0.0 20.39265 12-13 GTACTTT 6685 0.0 20.29572 14-15 ATGGGCG 385 0.0 19.747238 5 TAAGGTG 745 0.0 19.13428 5 ACCGTCC 225 1.6628965E-6 19.006718 8 GTACCTG 1385 0.0 18.526966 1 AGAGTAC 11015 0.0 18.420036 10-11 GTATCAA 14760 0.0 18.350538 1 CATGGGG 3730 0.0 18.344841 4 AGTACAT 5615 0.0 17.94099 2 GGTAAGG 825 0.0 17.855335 3 CATGGGA 3630 0.0 17.802975 4 TACCTGG 1315 0.0 17.706413 2 >>END_MODULE