FastQCFastQC Report
Thu 26 May 2016
SRR936377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652148
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82890.5017105005120606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64470.3902192781760472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63750.38586131508799454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38780.23472473410372438No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27400.16584470640644786No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20980.1269862022046451No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20410.1235361480932701No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20330.12305192997237537No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19960.12081242116323719No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.11324651302425691No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18000.10894907720131611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT77950.022.2130912-13
GTACTTT79850.021.3272714-15
GTACATG70300.021.2947961
TACATGG71150.020.6951392
AGTACTT79400.020.3416412-13
ACATGGG71900.019.6198643
TAAGGTG8100.019.3513165
GTAAGGT7300.018.8687694
ACTTTTT93650.018.6641616-17
GTACCTG12000.018.6137921
AGAGTAC127650.018.49621610-11
GCACCGT5750.018.173966
TACTTTT88350.018.03877314-15
TACCTGG11600.016.7883282
TGCACCG4601.0913936E-1116.521285
GTATCAA180100.016.4924261
GGTATCA151200.016.2187041
AGGCGAC4701.4551915E-1116.1697645
CATGGGG37900.016.0412864
GGTAAGG8650.015.9239313
Produced by FastQC (version 0.11.5)