##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936377_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1652148 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.336094587167736 31.0 31.0 34.0 30.0 34.0 2 31.482767887622657 31.0 31.0 34.0 30.0 34.0 3 31.566498279815125 31.0 31.0 34.0 30.0 34.0 4 35.40463687272569 37.0 35.0 37.0 33.0 37.0 5 35.128399513845004 37.0 35.0 37.0 32.0 37.0 6 35.26186515977987 37.0 35.0 37.0 32.0 37.0 7 35.19401227977155 37.0 35.0 37.0 32.0 37.0 8 35.15895730890937 37.0 35.0 37.0 32.0 37.0 9 36.68593007406116 39.0 35.0 39.0 32.0 39.0 10-11 36.50789517646119 38.0 35.0 39.0 32.0 39.0 12-13 36.38452396516535 38.0 35.0 39.0 32.0 39.0 14-15 37.28619046235568 39.0 36.0 40.0 32.0 41.0 16-17 37.17799585751398 39.0 36.0 40.0 32.0 41.0 18-19 37.355878529042194 39.0 36.0 40.0 32.0 41.0 20-21 37.3938845067149 39.0 36.0 40.0 32.0 41.0 22-23 37.2790331132562 39.0 36.0 40.0 31.5 41.0 24-25 37.10159440921758 39.0 36.0 40.0 31.0 41.0 26-27 36.7949469417994 39.0 36.0 40.0 30.5 41.0 28-29 36.66438115713604 38.5 35.5 40.0 30.0 41.0 30-31 36.60220724777683 38.5 35.0 40.0 30.0 41.0 32-33 36.40941610557892 38.0 35.0 40.0 30.0 41.0 34-35 36.175880732234646 38.0 35.0 40.0 30.0 41.0 36-37 35.855073819052535 38.0 34.5 40.0 29.5 41.0 38-39 35.621425864995146 38.0 34.0 40.0 28.0 41.0 40-41 35.344723051445754 38.0 34.0 40.0 27.0 41.0 42-43 35.012254047458214 38.0 33.5 40.0 26.0 41.0 44-45 34.699261204202045 38.0 33.0 40.0 25.5 41.0 46-47 34.26187302832434 38.0 33.0 40.0 24.0 41.0 48-49 33.8139249631389 37.0 33.0 40.0 23.0 41.0 50-51 33.38815499579941 37.0 32.0 40.0 22.0 41.0 52-53 33.035427818815265 37.0 31.5 39.0 20.5 40.5 54-55 32.68936862799217 36.0 31.0 39.0 19.0 40.0 56-57 32.27202345068359 36.0 31.0 39.0 18.0 40.0 58-59 31.832427542810933 35.0 30.0 39.0 15.0 40.0 60-61 31.355027818331045 35.0 29.5 38.0 13.0 40.0 62-63 30.866227480831014 34.5 29.0 38.0 8.5 40.0 64-65 30.36426367371446 34.0 28.0 38.0 8.0 40.0 66-67 29.78916235107266 34.0 28.0 37.0 7.0 39.0 68-69 29.39467892706949 34.0 27.5 36.5 4.0 39.0 70-71 28.858381331454567 33.0 26.0 36.0 2.0 39.0 72-73 28.31282185373223 33.0 26.0 35.5 2.0 38.0 74-75 27.70493291157935 32.0 26.0 35.0 2.0 37.5 76-77 26.57999495202609 30.5 24.5 34.0 2.0 36.0 78-79 27.31433745645063 32.0 26.0 35.0 2.0 36.0 80-81 27.04841182509073 32.0 25.0 35.0 2.0 36.0 82-83 26.599621220374928 32.0 24.5 34.5 2.0 36.0 84-85 26.04599860303072 31.5 23.5 34.0 2.0 35.0 86-87 25.534400671126317 31.0 21.5 34.0 2.0 35.0 88-89 24.9573134489162 31.0 19.5 34.0 2.0 35.0 90-91 24.24432708207739 31.0 17.5 34.0 2.0 35.0 92-93 23.49389794376775 30.0 9.0 34.0 2.0 35.0 94-95 22.588252384168975 29.5 2.0 34.0 2.0 35.0 96-97 21.46719361703673 29.0 2.0 34.0 2.0 35.0 98-99 20.12575084072371 28.0 2.0 33.0 2.0 34.5 100-101 16.894647755527956 21.5 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25237199465754756 1101 2 0.3657596499252982 1101 3 0.3009765414834824 1101 4 0.23656603672856846 1101 5 0.16547671058251723 1101 6 0.26041469587030264 1101 7 0.27291550905962936 1101 8 0.22379194912326028 1101 9 0.37717640282728837 1101 10-11 0.2785728032075454 1101 12-13 0.20563915293315915 1101 14-15 0.5102110297690245 1101 16-17 0.4884967105105602 1101 18-19 0.3277065852697234 1101 20-21 0.29722115112949155 1101 22-23 0.23565486502091204 1101 24-25 0.253232296855316 1101 26-27 0.19912210511605366 1101 28-29 0.20311351483143625 1101 30-31 0.28079695003999916 1101 32-33 0.41743126223519056 1101 34-35 0.6008645062323126 1101 36-37 0.4862171397999546 1101 38-39 0.555215027152272 1101 40-41 0.451536011021922 1101 42-43 0.561544551803479 1101 44-45 0.555686632766907 1101 46-47 0.5307597944043181 1101 48-49 0.5460757390761444 1101 50-51 0.3448025356336686 1101 52-53 0.44008484044157825 1101 54-55 0.459916345430635 1101 56-57 0.4260527929186182 1101 58-59 0.2937441638191842 1101 60-61 0.5471316628479137 1101 62-63 0.4234200479170127 1101 64-65 0.14209857130711612 1101 66-67 0.4049308253036408 1101 68-69 0.29968690561369726 1101 70-71 0.44712062281272225 1101 72-73 0.3891660503752874 1101 74-75 0.5961759886038145 1101 76-77 0.3013481846963124 1101 78-79 0.090596278732896 1101 80-81 0.2838781793833256 1101 82-83 0.48825476032498827 1101 84-85 0.41713021017119445 1101 86-87 0.38616197646391726 1101 88-89 0.1417776041426677 1101 90-91 0.001471838510902046 1101 92-93 0.07047478751547231 1101 94-95 0.2704204408294828 1101 96-97 0.4175804377849097 1101 98-99 0.716464745410299 1101 100-101 1.099087097621485 1102 1 0.1664181521967265 1102 2 0.2095214379298227 1102 3 0.24068411324028105 1102 4 0.18237765155659247 1102 5 0.14945145424700002 1102 6 0.16387308207105633 1102 7 0.22153052569142773 1102 8 0.1200575092892322 1102 9 0.2298906444513733 1102 10-11 0.3372320570054299 1102 12-13 0.2705042552959043 1102 14-15 0.23833570827798667 1102 16-17 0.22748671428272615 1102 18-19 0.23109531508008274 1102 20-21 0.041470748194065266 1102 22-23 0.1100686797049022 1102 24-25 0.2651178203284559 1102 26-27 0.15756831752302958 1102 28-29 -0.07686766722678584 1102 30-31 0.13454424951532928 1102 32-33 0.3014190798683316 1102 34-35 0.0914619651671913 1102 36-37 0.3570334698297941 1102 38-39 0.3339734716500615 1102 40-41 0.38321702333288243 1102 42-43 0.2794801078543685 1102 44-45 0.28341480566906796 1102 46-47 0.416705492425649 1102 48-49 0.18359888643048095 1102 50-51 0.012800889599581922 1102 52-53 0.041733875111169993 1102 54-55 0.1269234353795312 1102 56-57 0.00976905779293702 1102 58-59 -0.06241967658409919 1102 60-61 0.15760562636082298 1102 62-63 -0.08939029809307186 1102 64-65 0.3160005722673027 1102 66-67 0.10156224226464516 1102 68-69 -0.009579966235687465 1102 70-71 0.22178541482945846 1102 72-73 0.11513673896621768 1102 74-75 -0.025598384703002353 1102 76-77 -0.18332312287201447 1102 78-79 -0.10656774170535854 1102 80-81 0.3231590253762633 1102 82-83 0.41157271924272365 1102 84-85 0.1766463447344826 1102 86-87 -0.06530573726331923 1102 88-89 0.10854507610864772 1102 90-91 0.35443148495649623 1102 92-93 0.2949483995315205 1102 94-95 0.5439782320574942 1102 96-97 0.6406866412758738 1102 98-99 0.8609771078121362 1102 100-101 1.0924408063420366 1103 1 0.12966548557902158 1103 2 0.15935332977034022 1103 3 0.21033959141700365 1103 4 0.07828262751491621 1103 5 -0.03327045068359524 1103 6 0.12048843245749197 1103 7 0.03900410792348197 1103 8 -0.010757963789700398 1103 9 0.09451686435607343 1103 10-11 -0.019320867093711058 1103 12-13 0.11718629272576209 1103 14-15 0.2710421416417148 1103 16-17 0.36566805724712736 1103 18-19 0.22025698296359764 1103 20-21 0.13079353098082436 1103 22-23 0.15139733898698182 1103 24-25 0.14929505158220024 1103 26-27 0.27411700786704074 1103 28-29 0.33184806311756887 1103 30-31 0.23399078323632239 1103 32-33 0.1931430876359812 1103 34-35 0.18225171170350052 1103 36-37 0.19841615809149005 1103 38-39 0.18670710581042016 1103 40-41 0.339570734132856 1103 42-43 0.22053249301376354 1103 44-45 0.1759345325918673 1103 46-47 0.3663019356393207 1103 48-49 0.42727201461389797 1103 50-51 0.4939679695613961 1103 52-53 0.19309791108945262 1103 54-55 0.09858036188932573 1103 56-57 0.12925977593285154 1103 58-59 0.392803097778355 1103 60-61 0.3531520561820116 1103 62-63 0.3219441232001259 1103 64-65 0.2833613713669827 1103 66-67 0.48519243347485386 1103 68-69 0.5019288004455227 1103 70-71 0.3704239709461561 1103 72-73 0.31541482215518 1103 74-75 0.4327107377432462 1103 76-77 0.3676612251944995 1103 78-79 0.40950918735331854 1103 80-81 0.5223311480428379 1103 82-83 0.5579977342552702 1103 84-85 0.43844172691728645 1103 86-87 0.13224943115804777 1103 88-89 0.2519169542542663 1103 90-91 0.4051278674719505 1103 92-93 0.34718586351219827 1103 94-95 0.46565578732311863 1103 96-97 0.503734113335252 1103 98-99 0.9724344916106809 1103 100-101 1.1421156064267137 1104 1 0.16978358895514845 1104 2 0.27737218954041865 1104 3 0.15417216122188648 1104 4 0.0880686886273736 1104 5 -0.01624424649702405 1104 6 0.16394520631998688 1104 7 0.18840570193489725 1104 8 0.038561446297109114 1104 9 0.13855043491145125 1104 10-11 0.2699455556280199 1104 12-13 0.10391255988800197 1104 14-15 0.18177036804676305 1104 16-17 0.1212232926649719 1104 18-19 0.049747703314608316 1104 20-21 0.07268400821011767 1104 22-23 0.19967072195995428 1104 24-25 0.05672714008290569 1104 26-27 0.1597065574744434 1104 28-29 0.24369764046718956 1104 30-31 -0.0018250808679596275 1104 32-33 0.141334835491854 1104 34-35 0.1706267338273051 1104 36-37 0.21432018812223674 1104 38-39 0.18454206018393648 1104 40-41 0.25341316875272213 1104 42-43 0.2739963602133173 1104 44-45 0.6056354462191678 1104 46-47 0.3164297391881874 1104 48-49 0.30722201824975315 1104 50-51 0.30653936598838527 1104 52-53 0.4110672409817582 1104 54-55 0.4391491734329449 1104 56-57 0.4198377114013141 1104 58-59 0.2394307442310719 1104 60-61 0.25948831328735267 1104 62-63 0.3838806179391199 1104 64-65 0.4523670556407673 1104 66-67 0.23294239128527394 1104 68-69 0.6687837080880783 1104 70-71 0.46629556645306636 1104 72-73 0.32141520906447596 1104 74-75 0.5334705617406748 1104 76-77 0.6022303358382111 1104 78-79 0.6752629127272947 1104 80-81 0.7836749306711575 1104 82-83 0.7102362514871423 1104 84-85 0.7086502600508524 1104 86-87 0.7301550444984635 1104 88-89 0.6725045075743452 1104 90-91 0.933946435825245 1104 92-93 0.940168947892726 1104 94-95 0.9782300649895355 1104 96-97 1.2933696091073763 1104 98-99 1.4990863679149555 1104 100-101 1.7306780463151377 1105 1 0.057446777860434395 1105 2 0.0994492917661276 1105 3 0.10322082428851687 1105 4 0.09101582836058242 1105 5 -0.050798919578923574 1105 6 0.06486853348263821 1105 7 0.23319915475762798 1105 8 0.1522893619436232 1105 9 -0.13122631157595066 1105 10-11 0.009255775924152942 1105 12-13 -0.04960629196733635 1105 14-15 0.1656654607918 1105 16-17 0.26008791589800495 1105 18-19 0.2470749740254945 1105 20-21 0.35903642515474843 1105 22-23 0.23525251475589926 1105 24-25 0.16385996326873453 1105 26-27 0.342049169577507 1105 28-29 0.3415596560901051 1105 30-31 0.44824743173643355 1105 32-33 0.3673341587075498 1105 34-35 0.35842627486517387 1105 36-37 0.07831938621814061 1105 38-39 0.332540285757851 1105 40-41 0.3096600585307243 1105 42-43 0.2542784768989037 1105 44-45 0.322797757864123 1105 46-47 0.6182698531609461 1105 48-49 0.15854359601028278 1105 50-51 0.35556400036846014 1105 52-53 0.22847950783951632 1105 54-55 0.14757144134284061 1105 56-57 0.1275611568590449 1105 58-59 0.14962999749342387 1105 60-61 0.24777070296155657 1105 62-63 0.09512021380220403 1105 64-65 0.189546400638676 1105 66-67 0.4035338382103184 1105 68-69 0.5077539722330116 1105 70-71 0.3977852312742165 1105 72-73 0.45983921158962104 1105 74-75 0.08316984770479863 1105 76-77 -0.11884822517048477 1105 78-79 0.09697375521001561 1105 80-81 0.2960725954809327 1105 82-83 0.31818619219742317 1105 84-85 0.25978287849521564 1105 86-87 0.35179053074523736 1105 88-89 0.6503590160099755 1105 90-91 0.7037978276407024 1105 92-93 0.5277719939908394 1105 94-95 0.4551545637283816 1105 96-97 0.89932772810198 1105 98-99 1.2055203686486102 1105 100-101 0.9105789741921129 1106 1 -0.05599978030246788 1106 2 0.0929838554006217 1106 3 0.0551809736678166 1106 4 0.1442058753896447 1106 5 -0.18540068574100133 1106 6 -0.104750815615823 1106 7 0.03439757864862969 1106 8 -0.0176519879174748 1106 9 -0.09181511297047962 1106 10-11 0.02096976419412755 1106 12-13 -7.870098093576416E-5 1106 14-15 0.04312903899501919 1106 16-17 0.13378432728424627 1106 18-19 -0.03881297939334161 1106 20-21 -0.11029814695345408 1106 22-23 0.07431477832441402 1106 24-25 -0.22137884387580442 1106 26-27 -0.21150343094181068 1106 28-29 -0.10812863264531813 1106 30-31 -0.06633702385832407 1106 32-33 -0.1489274850700255 1106 34-35 -0.16568171644322405 1106 36-37 -0.1781863002426931 1106 38-39 -0.013104532874613994 1106 40-41 0.007935962342074276 1106 42-43 -0.04441959896496428 1106 44-45 0.10005251978329 1106 46-47 0.08478631333150588 1106 48-49 0.08959639324059054 1106 50-51 0.11192654175342653 1106 52-53 -0.23850240080184903 1106 54-55 -0.2525801774373022 1106 56-57 -0.028437923138781684 1106 58-59 -0.07178789533842433 1106 60-61 -0.03178648546336049 1106 62-63 0.05474089448276587 1106 64-65 0.11465096009684217 1106 66-67 0.2080996516556617 1106 68-69 0.09984134822943247 1106 70-71 0.11201984393514053 1106 72-73 0.023863192172736802 1106 74-75 0.127056977963786 1106 76-77 -0.04637081667135945 1106 78-79 0.28542288574742614 1106 80-81 0.33425631749501505 1106 82-83 0.7635332206509133 1106 84-85 0.4928699700172743 1106 86-87 0.3877489974889734 1106 88-89 0.579324255664492 1106 90-91 0.6635861666130829 1106 92-93 0.39874531998029283 1106 94-95 0.7996182085107009 1106 96-97 0.942033079246233 1106 98-99 1.3976743237500848 1106 100-101 1.4862851666541488 1107 1 0.1555229209457636 1107 2 0.13149348761926305 1107 3 0.09261997382238718 1107 4 0.041949157126126124 1107 5 0.1308852872124504 1107 6 0.1596959351073437 1107 7 -0.15999185508984226 1107 8 -0.029998955984623876 1107 9 0.14784275163273009 1107 10-11 -0.011383260483405877 1107 12-13 0.035263828726428414 1107 14-15 0.30175166485106786 1107 16-17 0.2428735014086456 1107 18-19 0.19862617848545483 1107 20-21 0.2465327316651411 1107 22-23 0.11020823876244634 1107 24-25 0.08149820377899175 1107 26-27 0.1980885680235147 1107 28-29 -0.12584327166187137 1107 30-31 -0.05211518788520664 1107 32-33 0.05108702555900635 1107 34-35 0.03419462776151505 1107 36-37 0.07244207308424677 1107 38-39 0.09850365490553514 1107 40-41 0.09758294397351364 1107 42-43 -0.23037725316405044 1107 44-45 -0.13981969204665745 1107 46-47 0.1702313637874866 1107 48-49 0.025329290413850458 1107 50-51 0.1379324280216352 1107 52-53 0.1458705980720083 1107 54-55 0.12407931803442551 1107 56-57 -0.07470427519818656 1107 58-59 -0.06963022789049944 1107 60-61 -0.0395624476600176 1107 62-63 0.0726810791462924 1107 64-65 0.23146467831935524 1107 66-67 0.14300165759240357 1107 68-69 0.20202204341473973 1107 70-71 0.1728819961137873 1107 72-73 0.32481379609880534 1107 74-75 0.09812152187263834 1107 76-77 0.042229980151443414 1107 78-79 0.3120388493320583 1107 80-81 0.47829576117011285 1107 82-83 0.665072924570989 1107 84-85 0.47917509069208464 1107 86-87 0.6147767790346101 1107 88-89 0.6419606476912172 1107 90-91 0.5175439406825895 1107 92-93 0.7604307926324907 1107 94-95 1.1187802827735744 1107 96-97 1.4381142630612018 1107 98-99 1.553080952583965 1107 100-101 1.854073936058171 1108 1 -0.06866573305511992 1108 2 0.08498070729786988 1108 3 0.03584401055480768 1108 4 -0.005198694520167635 1108 5 -0.020083359564004866 1108 6 0.0991339947884029 1108 7 0.12470103511062547 1108 8 0.19626998042087251 1108 9 0.18408061257688502 1108 10-11 0.12090783430392804 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66-67 -2.590401585127612 2316 68-69 -2.1855313969337296 2316 70-71 -1.5776221581170766 2316 72-73 -1.2455258021911035 2316 74-75 -1.2345799381580171 2316 76-77 -1.3826308732808386 2316 78-79 -1.684210243190325 2316 80-81 -2.302659308604831 2316 82-83 -1.918455348398279 2316 84-85 -1.9998520933040425 2316 86-87 -2.087691560079694 2316 88-89 -1.6325815802671606 2316 90-91 -2.0267812992948215 2316 92-93 -1.9149745473691233 2316 94-95 -2.148824782968134 2316 96-97 -2.3335643688658756 2316 98-99 -1.9643829499731034 2316 100-101 -1.7428446732828 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 28.0 4 51.0 5 110.0 6 232.0 7 625.0 8 1367.0 9 2366.0 10 3655.0 11 5570.0 12 7557.0 13 10073.0 14 12800.0 15 16616.0 16 20125.0 17 24071.0 18 21617.0 19 17781.0 20 17853.0 21 18712.0 22 20520.0 23 22634.0 24 26283.0 25 31310.0 26 37666.0 27 44725.0 28 53214.0 29 62997.0 30 74289.0 31 87245.0 32 103404.0 33 124100.0 34 150104.0 35 183684.0 36 211891.0 37 179953.0 38 55657.0 39 1254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.981162919674794 20.182352528038866 15.449348403643418 22.387136148642924 2 17.179230932663785 21.577758098559794 36.9453038128922 24.297707155884225 3 21.252699600991708 23.71835534981018 29.89808098318742 25.130864066010695 4 14.447426431868445 17.22297478061813 35.19273767024868 33.136861117264736 5 13.851281008343138 37.69750190056993 33.56799924461424 14.883217846472688 6 32.94412237175165 36.10601000153739 15.71501550105136 15.234852125659595 7 29.070216469710946 32.79573016460995 19.525066761573417 18.608986604105684 8 27.36195546645942 34.06032631459167 18.48986894636558 20.087849272583327 9 26.246745849728924 16.52070278145487 19.77930187922071 37.4532494895955 10-11 26.13585224743295 26.149985427994775 25.859604555423704 21.85455776914857 12-13 27.155440875522736 23.325210500607398 26.80195843797079 22.717390185899077 14-15 23.271989816701844 25.08139556702098 25.697635215907834 25.948979400369343 16-17 22.39125506964875 28.14404475726971 26.54702784890729 22.917672324174244 18-19 22.33649769875338 26.492632621290586 29.04379631848963 22.127073361466408 20-21 23.211994324963623 25.146929936059 29.142849187845155 22.498226551132223 22-23 22.758869899465445 25.248279708749706 29.338573473997176 22.654276917787676 24-25 23.08739436033623 25.52269408569202 29.291569254980292 22.098342298991458 26-27 22.640692850320264 25.470045530648143 29.56190219082196 22.327359428209633 28-29 22.12757225559486 26.357311997443176 29.33893727316089 22.176178473801066 30-31 22.456323845892413 25.526418447926208 29.226275269250664 22.790982436930715 32-33 22.31197114046025 25.726784655053446 29.313103005798542 22.648141198687764 34-35 23.225761540196036 25.555365549887334 29.0373926110663 22.18148029885033 36-37 23.27246015185607 25.226473538386852 29.186317578099697 22.31474873165738 38-39 22.644989494545698 25.662031272416026 29.36260857300761 22.330370660030667 40-41 22.82500238329785 25.792669732419967 28.94749027387497 22.434837610407218 42-43 22.722723540660965 25.933401063527473 28.453642192702993 22.89023320310857 44-45 22.999240376845716 25.61500724819703 28.48269057915969 22.90306179579756 46-47 23.526682696308896 26.32283134526427 27.81573679105528 22.334749167371555 48-49 23.522680742152826 26.307463746251454 27.528728657598222 22.6411268539975 50-51 22.990133384822204 26.588684836669113 27.842122051621626 22.579059726887056 52-53 23.572541863005906 26.6445435093804 27.216900760540273 22.566013867073416 54-55 22.95976054863175 26.433167283050157 27.428167615957776 23.178904552360315 56-57 23.395999721572387 26.549373991834806 27.18222179180273 22.872404494790075 58-59 23.93854866108218 26.122871808715566 27.03036341125567 22.90821611894659 60-61 23.089541027405676 26.452164302104652 27.95539987518938 22.502894795300293 62-63 23.046148407797915 26.45224241154954 27.82390473117876 22.677704449473783 64-65 22.775896248543454 26.843116780920383 27.456878679196745 22.92410829133942 66-67 22.917127566753212 26.630859963144133 27.173287032987787 23.278725437114865 68-69 23.04458909465967 26.43204923649059 27.41137383270343 23.111987836146305 70-71 23.31165952192525 26.50447372283649 27.187947807323496 22.99591894791476 72-73 23.233119569526703 26.347453446600916 27.413346972251055 23.00608001162133 74-75 22.88283096877524 27.34895393386662 26.84080370662148 22.927411390736655 76-77 23.2722488766648 26.674016856610468 26.82887928568986 23.224854981034877 78-79 23.202946794096363 26.82751442784504 26.92968738887253 23.03985138918607 80-81 23.417508707927823 26.81984266562314 26.8694768706118 22.89317175583724 82-83 23.381137543687196 26.736229227776278 27.026680209141656 22.855953019394875 84-85 23.079809887680714 27.13528312256821 26.799826400238246 22.985080589512833 86-87 23.229830845795234 27.081348488168405 26.557530951478782 23.131289714557575 88-89 23.018160499921457 27.182548390015977 26.29442328011261 23.504867829949948 90-91 23.161663111831864 26.902063620192912 26.461348851723287 23.474924416251934 92-93 23.40937635241672 27.029237925472195 26.62300598922001 22.938379732891075 94-95 23.09318424000201 26.768765818695318 26.700974112344777 23.437075828957894 96-97 22.854394894357778 26.63683855960749 26.962811105401318 23.545955440633414 98-99 22.87627175223299 27.006982928701976 26.19211119420398 23.92463412486105 100-101 22.818459236473217 26.777611422182957 26.252940604999687 24.150988736344136 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 341.0 1 253.5 2 215.5 3 324.5 4 326.0 5 297.5 6 508.5 7 1067.5 8 1607.0 9 1891.0 10 1823.5 11 1532.5 12 1491.0 13 1614.5 14 1773.5 15 1998.5 16 2164.0 17 2347.0 18 2590.5 19 2831.5 20 3130.5 21 3574.0 22 4148.0 23 4904.5 24 5856.5 25 7121.0 26 8946.0 27 11125.0 28 13113.5 29 15084.5 30 17742.0 31 20997.5 32 24946.5 33 27219.5 34 28291.5 35 30326.0 36 33065.5 37 35729.5 38 37509.0 39 38596.0 40 40102.0 41 42954.0 42 45181.5 43 47508.0 44 50451.5 45 53953.5 46 56269.0 47 61589.5 48 73714.0 49 73594.5 50 62928.5 51 58012.5 52 56553.0 53 57297.0 54 58218.5 55 55676.5 56 51331.0 57 47690.5 58 44882.0 59 40913.0 60 34974.5 61 29635.0 62 25094.5 63 19774.5 64 14780.5 65 11376.0 66 8682.0 67 6930.5 68 5411.0 69 3965.5 70 3150.5 71 2490.5 72 1959.5 73 1681.0 74 1399.5 75 1146.0 76 845.0 77 547.5 78 401.5 79 280.0 80 178.0 81 138.0 82 100.0 83 58.5 84 29.5 85 17.0 86 12.5 87 5.0 88 1.5 89 1.5 90 2.5 91 2.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030989959737263248 2 0.005750090185625017 3 0.0031474177858157987 4 3.631635906710537E-4 5 7.263271813421074E-4 6 1.2105453022368456E-4 7 0.0 8 0.0 9 0.0016342361580197415 10-11 4.5395448833881715E-4 12-13 9.079089766776343E-4 14-15 0.001876345218467111 16-17 0.002209245176582243 18-19 0.0 20-21 0.0 22-23 0.0017855543207993473 24-25 6.355362836743439E-4 26-27 0.004479017618276329 28-29 0.005810617450736859 30-31 9.986998743453975E-4 32-33 4.8421812089473825E-4 34-35 0.009109353399332262 36-37 0.011439653106138191 38-39 0.009018562501664499 40-41 0.0015434452603519783 42-43 2.723726930032902E-4 44-45 7.868544464539497E-4 46-47 3.026363255592114E-5 48-49 5.144817534506594E-4 50-51 6.657999162302651E-4 52-53 0.001997399748690795 54-55 0.002300036074250007 56-57 7.868544464539497E-4 58-59 6.960635487861862E-4 60-61 0.002723726930032903 62-63 0.0039342722322697485 64-65 0.007444853608756601 66-67 0.002209245176582243 68-69 0.002421090604473691 70-71 3.026363255592114E-5 72-73 7.868544464539497E-4 74-75 0.004690863046167777 76-77 0.002209245176582243 78-79 0.0028750450928125087 80-81 0.0037224268043783005 82-83 0.003752690436934222 84-85 0.004357963088052644 86-87 0.002784254195144745 88-89 0.007777753566871734 90-91 0.010440953231792793 92-93 7.868544464539497E-4 94-95 0.001725027055687505 96-97 9.079089766776343E-4 98-99 0.0028750450928125087 100-101 0.003904008599713827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1652148.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.917552107892625 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87774292218953 46.26323337956763 2 11.917582615484228 13.804744242648459 3 3.460817540035846 6.013262406328046 4 1.4930322799689564 3.45891099495471 5 0.8039094615619928 2.328023406502231 6 0.5019846754203006 1.7444234157611291 7 0.3317626328518032 1.345041570295217 8 0.2511071303991641 1.1634808247645578 9 0.1758943899042328 0.9168635243487948 >10 1.066718953109565 11.391697274165088 >50 0.07581552476013152 2.9903264190015713 >100 0.03798204241003192 4.0114546700431974 >500 0.003348048535863465 1.3061708645845045 >1k 0.0019879038181689324 1.9831012625559035 >5k 3.1387955023719986E-4 1.2792657444789366 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8289 0.5017105005120606 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6447 0.3902192781760472 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6375 0.38586131508799454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3878 0.23472473410372438 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2740 0.16584470640644786 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2098 0.1269862022046451 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2041 0.1235361480932701 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2033 0.12305192997237537 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1996 0.12081242116323719 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1871 0.11324651302425691 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1800 0.10894907720131611 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.026363255592114E-5 0.0 0.0 0.0 0.0 12-13 6.052726511184228E-5 0.0 0.0 0.0 0.0 14-15 6.052726511184228E-5 0.0 0.0 0.0 0.0 16-17 1.2105453022368456E-4 0.0 0.0 0.0 6.052726511184228E-5 18-19 1.2105453022368456E-4 0.0 0.0 0.0 6.052726511184228E-5 20-21 1.2105453022368456E-4 0.0 0.0 0.0 6.052726511184228E-5 22-23 1.2105453022368456E-4 0.0 0.0 3.026363255592114E-5 6.052726511184228E-5 24-25 1.2105453022368456E-4 0.0 0.0 9.079089766776342E-5 6.052726511184228E-5 26-27 1.2105453022368456E-4 0.0 0.0 2.4210906044736912E-4 6.052726511184228E-5 28-29 1.5131816277960572E-4 0.0 0.0 7.263271813421074E-4 6.052726511184228E-5 30-31 1.8158179533552684E-4 0.0 0.0 0.001967136116134874 6.052726511184228E-5 32-33 1.8158179533552684E-4 0.0 0.0 0.004660599413611855 6.052726511184228E-5 34-35 1.8158179533552684E-4 0.0 0.0 0.00904882613422042 6.052726511184228E-5 36-37 1.8158179533552684E-4 0.0 0.0 0.01510155264540465 6.052726511184228E-5 38-39 1.8158179533552684E-4 0.0 0.0 0.0233332607006152 6.052726511184228E-5 40-41 1.8158179533552684E-4 0.0 0.0 0.035045286499756675 6.052726511184228E-5 42-43 2.1184542789144797E-4 0.0 0.0 0.047453375847684345 6.052726511184228E-5 44-45 2.4210906044736912E-4 0.0 0.0 0.06361415563254624 6.052726511184228E-5 46-47 2.723726930032903E-4 0.0 0.0 0.08286182593811209 6.052726511184228E-5 48-49 3.026363255592114E-4 0.0 0.0 0.10298714158779965 6.052726511184228E-5 50-51 3.026363255592114E-4 0.0 0.0 0.1275612112232076 1.2105453022368456E-4 52-53 3.026363255592114E-4 0.0 0.0 0.15567612586765833 1.2105453022368456E-4 54-55 3.026363255592114E-4 0.0 0.0 0.1878161036420466 1.2105453022368456E-4 56-57 3.026363255592114E-4 0.0 0.0 0.2277640986158625 1.2105453022368456E-4 58-59 3.026363255592114E-4 0.0 0.0 0.2748240472403199 1.2105453022368456E-4 60-61 3.026363255592114E-4 0.0 0.0 0.32823935870152066 1.2105453022368456E-4 62-63 3.631635906710537E-4 0.0 0.0 0.37938489772102746 1.2105453022368456E-4 64-65 4.2369085578289594E-4 0.0 0.0 0.43382917268912957 1.2105453022368456E-4 66-67 4.8421812089473825E-4 0.0 0.0 0.5058566181722218 1.2105453022368456E-4 68-69 4.8421812089473825E-4 0.0 0.0 0.5925013981798242 1.2105453022368456E-4 70-71 5.144817534506594E-4 0.0 0.0 0.6829896595220284 1.2105453022368456E-4 72-73 5.447453860065806E-4 0.0 0.0 0.7793188019475252 1.2105453022368456E-4 74-75 5.750090185625016E-4 0.0 0.0 0.8851204613630256 1.5131816277960572E-4 76-77 6.052726511184228E-4 0.0 0.0 0.9997288378522989 1.8158179533552684E-4 78-79 6.052726511184228E-4 0.0 0.0 1.135612548028385 1.8158179533552684E-4 80-81 6.657999162302651E-4 0.0 0.0 1.291772892016938 1.8158179533552684E-4 82-83 6.960635487861862E-4 0.0 0.0 1.4525938354191028 1.8158179533552684E-4 84-85 7.263271813421074E-4 0.0 0.0 1.615260860407179 1.8158179533552684E-4 86-87 7.263271813421074E-4 0.0 0.0 1.7969637102729297 1.8158179533552684E-4 88-89 7.868544464539497E-4 0.0 0.0 2.0019090299416273 1.8158179533552684E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 7795 0.0 22.21309 12-13 GTACTTT 7985 0.0 21.32727 14-15 GTACATG 7030 0.0 21.294796 1 TACATGG 7115 0.0 20.695139 2 AGTACTT 7940 0.0 20.34164 12-13 ACATGGG 7190 0.0 19.619864 3 TAAGGTG 810 0.0 19.351316 5 GTAAGGT 730 0.0 18.868769 4 ACTTTTT 9365 0.0 18.66416 16-17 GTACCTG 1200 0.0 18.613792 1 AGAGTAC 12765 0.0 18.496216 10-11 GCACCGT 575 0.0 18.17396 6 TACTTTT 8835 0.0 18.038773 14-15 TACCTGG 1160 0.0 16.788328 2 TGCACCG 460 1.0913936E-11 16.52128 5 GTATCAA 18010 0.0 16.492426 1 GGTATCA 15120 0.0 16.218704 1 AGGCGAC 470 1.4551915E-11 16.169764 5 CATGGGG 3790 0.0 16.041286 4 GGTAAGG 865 0.0 15.923931 3 >>END_MODULE