FastQCFastQC Report
Thu 26 May 2016
SRR936376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600190
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61610.38501677925746314No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54200.3387097782138371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53440.33396034220936266No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47270.29540242096251074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31640.19772651997575288No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31420.1963516832376155No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28770.17979114980096114No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17970.11229916447421867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGT350.00840362127.1363342-43
GTACATG71400.024.691641
TACATGG72500.023.5952842
GAGTACT58450.023.1708212-13
ACATGGG72250.022.8891283
CTATCCG1050.00218969922.6312894
GTAAGGT6200.022.2297674
TAAGGTG7750.022.0764585
GTACTTT61200.021.9722914-15
GTACCTG12300.021.6350141
CATGGGG37500.020.7845754
TAGCCCT9650.020.1922684
TACCTGG12350.020.0076962
GGTAAGG6900.019.9726983
TCGCGCT2450.019.38248860-61
GTATAGC5400.019.3599031
AGTACTT61300.019.26402312-13
ATACCGT1507.6931936E-419.0108786
TACCGTC1250.00601224419.0108787
GTATCAA137550.018.93191
Produced by FastQC (version 0.11.5)