##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936376_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1600190 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81300533061699 33.0 31.0 34.0 30.0 34.0 2 31.90682168992432 33.0 31.0 34.0 30.0 34.0 3 31.877041476324685 33.0 31.0 34.0 30.0 34.0 4 35.54115323805298 37.0 35.0 37.0 33.0 37.0 5 35.5753491772852 37.0 35.0 37.0 33.0 37.0 6 35.65832244920916 37.0 35.0 37.0 33.0 37.0 7 35.54608390253657 37.0 35.0 37.0 33.0 37.0 8 35.53746305126266 37.0 35.0 37.0 33.0 37.0 9 37.118966497728394 39.0 37.0 39.0 34.0 39.0 10-11 37.00637799261338 39.0 37.0 39.0 33.0 39.0 12-13 36.92769858579294 39.0 37.0 39.0 33.0 39.0 14-15 38.02005480599179 40.0 38.0 41.0 33.0 41.0 16-17 37.93645223379724 40.0 38.0 41.0 33.0 41.0 18-19 37.821724295239946 40.0 38.0 41.0 33.0 41.0 20-21 37.79047988051419 40.0 37.5 41.0 33.0 41.0 22-23 37.78799361325842 40.0 37.5 41.0 33.0 41.0 24-25 37.89803492085315 40.0 38.0 41.0 33.0 41.0 26-27 37.8239902761547 40.0 37.5 41.0 33.0 41.0 28-29 37.716637086845935 40.0 37.0 41.0 33.0 41.0 30-31 37.52286853436155 40.0 37.0 41.0 32.5 41.0 32-33 37.344893731369396 39.5 36.5 41.0 32.0 41.0 34-35 37.14636136958737 39.0 36.0 41.0 31.5 41.0 36-37 36.944633137314945 39.0 36.0 41.0 31.0 41.0 38-39 36.737559602297225 39.0 36.0 41.0 31.0 41.0 40-41 36.507023540954506 39.0 35.0 40.0 30.0 41.0 42-43 36.226901180484816 39.0 35.0 40.0 30.0 41.0 44-45 36.07529605859304 39.0 35.0 40.0 30.0 41.0 46-47 35.91145395234316 39.0 35.0 40.0 29.0 41.0 48-49 35.647530293277676 39.0 35.0 40.0 28.0 41.0 50-51 34.652331910585616 38.0 34.0 39.5 26.0 40.5 52-53 34.758624600828654 38.0 34.0 39.5 26.5 40.5 54-55 35.17975365425356 38.0 35.0 40.0 27.0 41.0 56-57 35.004308550859584 38.0 34.5 40.0 26.5 41.0 58-59 34.76592748361132 38.0 34.0 40.0 26.0 41.0 60-61 34.45642361219605 37.5 34.0 40.0 26.0 41.0 62-63 34.12372312037945 37.0 34.0 40.0 26.0 41.0 64-65 33.76052874971097 36.5 33.5 39.5 25.0 41.0 66-67 33.38956905117517 36.0 33.0 39.0 23.5 41.0 68-69 32.94812990957324 35.5 33.0 39.0 22.5 40.0 70-71 32.54188377630156 35.0 32.0 38.0 22.0 40.0 72-73 32.137875814747 35.0 32.0 37.0 20.5 39.5 74-75 31.75039120354458 35.0 31.5 37.0 20.0 39.0 76-77 31.308289328142283 35.0 31.0 36.0 18.5 39.0 78-79 30.8584015023216 34.5 31.0 36.0 17.5 38.5 80-81 30.427199895012468 34.0 30.5 35.0 12.5 37.0 82-83 30.041878151969456 34.0 30.0 35.0 8.5 37.0 84-85 29.696880682918902 34.0 30.0 35.0 7.0 36.0 86-87 29.382333347914937 34.0 29.0 35.0 3.5 36.0 88-89 29.041309157037603 34.0 29.0 35.0 2.0 36.0 90-91 28.69673070072929 34.0 29.0 35.0 2.0 35.0 92-93 28.289854017335443 34.0 29.0 35.0 2.0 35.0 94-95 27.821876777132715 34.0 27.0 35.0 2.0 35.0 96-97 27.25881957767515 33.0 26.5 35.0 2.0 35.0 98-99 26.5483273861229 33.0 25.0 35.0 2.0 35.0 100-101 23.873006330498253 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38673971012134345 1101 2 0.3367577409783067 1101 3 0.37355037271612446 1101 4 0.48245496042120095 1101 5 0.3854776828440194 1101 6 0.3656982474673498 1101 7 0.46260740207916484 1101 8 0.40725237699191297 1101 9 0.5516014199538404 1101 10-11 0.6132036397005507 1101 12-13 0.6421223292389087 1101 14-15 0.7800009621916288 1101 16-17 0.814374302737292 1101 18-19 0.9293261322885513 1101 20-21 0.9229656950778775 1101 22-23 0.8024398416382894 1101 24-25 0.7594066869712961 1101 26-27 0.6135471540533288 1101 28-29 0.5721986237302659 1101 30-31 0.4223786607997084 1101 32-33 0.38115703980032833 1101 34-35 0.3364244434200998 1101 36-37 0.27631676153066564 1101 38-39 0.4784073360679315 1101 40-41 0.3840217996858897 1101 42-43 0.5154024617186153 1101 44-45 0.3364638191195155 1101 46-47 0.3817423967616449 1101 48-49 0.5661357985207616 1101 50-51 0.41966724288909774 1101 52-53 0.5119893424318676 1101 54-55 0.5539609854402414 1101 56-57 0.6880070527273787 1101 58-59 0.6046006721128876 1101 60-61 0.44325696432512274 1101 62-63 0.4235563685464854 1101 64-65 0.19685834459582452 1101 66-67 0.3700530912662714 1101 68-69 0.4145697638306771 1101 70-71 0.2817546877306043 1101 72-73 0.4913484946549147 1101 74-75 0.41785092829444537 1101 76-77 0.3621156504639522 1101 78-79 0.3058324791175515 1101 80-81 0.319580390835867 1101 82-83 0.3373182398719763 1101 84-85 0.38636342018182646 1101 86-87 0.46183507080012376 1101 88-89 0.6592177757064057 1101 90-91 0.5282381134884808 1101 92-93 0.6173178925933058 1101 94-95 0.5078474134056989 1101 96-97 0.4028152449516291 1101 98-99 0.5352858752490057 1101 100-101 1.108601742909812 1102 1 0.12389065268652999 1102 2 0.1267220644455378 1102 3 0.32725676806495585 1102 4 0.19881991813790023 1102 5 0.20046843337256348 1102 6 0.31118362449342385 1102 7 0.2575906649403379 1102 8 0.1603751747367994 1102 9 0.28651377020049296 1102 10-11 0.3691607397710257 1102 12-13 0.26331022564482254 1102 14-15 0.34151985754046166 1102 16-17 0.24950742905863876 1102 18-19 0.1645825848960243 1102 20-21 0.3325411002928149 1102 22-23 0.06391744470382577 1102 24-25 0.2538503973307158 1102 26-27 0.09361289136833761 1102 28-29 0.019661625844427988 1102 30-31 -0.03382288958788138 1102 32-33 0.01387759564951807 1102 34-35 -0.15833363885614915 1102 36-37 -0.28242674181676364 1102 38-39 -0.3615746054401754 1102 40-41 -0.10103865221686448 1102 42-43 -0.2712843555822957 1102 44-45 -0.1365954435654686 1102 46-47 0.04686418146918214 1102 48-49 0.27058490440519023 1102 50-51 0.11642717505964129 1102 52-53 -0.16948859097193747 1102 54-55 -0.006157275659127492 1102 56-57 -0.021095418202854432 1102 58-59 -0.12192383898646852 1102 60-61 -0.07362992785246547 1102 62-63 -0.09840727222166379 1102 64-65 -0.03791526357893815 1102 66-67 -0.05044109478024694 1102 68-69 -0.06405184469645775 1102 70-71 -0.2793771612827882 1102 72-73 0.00463304803758291 1102 74-75 -0.1675618844257798 1102 76-77 0.04229700617219834 1102 78-79 -0.11213461919111722 1102 80-81 -0.024814869911136128 1102 82-83 -0.13858908923302948 1102 84-85 -0.1251984191416433 1102 86-87 -0.3461403960779599 1102 88-89 -0.31550969522382744 1102 90-91 -0.012997461740550165 1102 92-93 0.01777100658203068 1102 94-95 -0.25743568648859494 1102 96-97 -0.2566296766480889 1102 98-99 -0.2023198329962348 1102 100-101 0.5478976819513868 1103 1 0.2170575727464623 1103 2 0.29983979316050835 1103 3 0.3186452529935693 1103 4 0.39517270766724977 1103 5 0.3462924021721392 1103 6 0.38788356279766845 1103 7 0.42818933407483684 1103 8 0.28554814318383137 1103 9 0.31657777954299604 1103 10-11 0.3202475765416892 1103 12-13 0.33539344880717437 1103 14-15 0.5487341290899082 1103 16-17 0.5851974574827565 1103 18-19 0.6739681888533653 1103 20-21 0.6884925369224675 1103 22-23 0.439403711890499 1103 24-25 0.300960061529544 1103 26-27 0.4053952651123893 1103 28-29 0.4969619960189391 1103 30-31 0.4273440414891496 1103 32-33 0.5652749768851919 1103 34-35 0.5235879910134003 1103 36-37 0.5664653512116189 1103 38-39 0.5086401178103088 1103 40-41 0.6224314859375539 1103 42-43 0.6416289233265715 1103 44-45 0.7800834796233147 1103 46-47 0.8110214285166037 1103 48-49 0.8777048420484093 1103 50-51 0.8319060607902955 1103 52-53 0.9050247287001909 1103 54-55 0.8605245395180319 1103 56-57 0.8250561715644622 1103 58-59 0.8126052626793125 1103 60-61 0.7179023302120484 1103 62-63 0.8101275363011595 1103 64-65 0.6788362347464556 1103 66-67 0.7630868005132498 1103 68-69 0.8761349502092415 1103 70-71 0.8080800097466536 1103 72-73 0.7610941631882966 1103 74-75 0.6664626341270115 1103 76-77 0.6279151480283574 1103 78-79 0.6059948427337929 1103 80-81 0.7295154006688023 1103 82-83 0.5683230383890425 1103 84-85 0.7372586141740634 1103 86-87 0.6031966500175763 1103 88-89 0.5207512943319621 1103 90-91 0.7020534097119402 1103 92-93 0.7505076263158585 1103 94-95 0.8784344714966004 1103 96-97 1.043002489974512 1103 98-99 1.2804075599175668 1103 100-101 1.9448666027164165 1104 1 0.24293614137511454 1104 2 0.24244648120057377 1104 3 0.35925902151140576 1104 4 0.32693829035423505 1104 5 0.22609475892929254 1104 6 0.2059498846560217 1104 7 0.1781390035759003 1104 8 0.2371271188866757 1104 9 0.3259501876624924 1104 10-11 0.2611996669537646 1104 12-13 0.374547632855851 1104 14-15 0.4361762069572208 1104 16-17 0.4672160385496227 1104 18-19 0.25950045561005197 1104 20-21 0.4373705453264023 1104 22-23 0.2247580133676621 1104 24-25 0.44814718948341437 1104 26-27 0.0617489929059829 1104 28-29 0.0305046799271409 1104 30-31 0.06359669330359452 1104 32-33 -0.05839645215889533 1104 34-35 0.11031501128523757 1104 36-37 0.0774969834395165 1104 38-39 0.08446308455629747 1104 40-41 0.17405165133562406 1104 42-43 0.09555380498681387 1104 44-45 0.13858930590430418 1104 46-47 0.2211378868421079 1104 48-49 0.20932899463141297 1104 50-51 0.06004986638166088 1104 52-53 0.07714283797822219 1104 54-55 0.2041572126759732 1104 56-57 0.161209227873492 1104 58-59 0.20111185162858902 1104 60-61 0.1766219279269663 1104 62-63 0.1385810076475451 1104 64-65 0.20773911959532398 1104 66-67 0.2636694566513569 1104 68-69 0.24538556693309488 1104 70-71 0.11801508200105815 1104 72-73 0.3635830414097967 1104 74-75 0.3103474364473193 1104 76-77 0.33945451545109506 1104 78-79 0.17626841633380508 1104 80-81 0.31938029622530806 1104 82-83 0.27712759510772855 1104 84-85 0.31863111426238433 1104 86-87 0.1351587599842503 1104 88-89 0.3193234503223046 1104 90-91 0.29208135365358245 1104 92-93 0.17626313056577203 1104 94-95 0.17125184665032478 1104 96-97 0.2862004422101663 1104 98-99 0.23525970040779143 1104 100-101 0.977510067069808 1105 1 0.2659095982097206 1105 2 0.2996218756645277 1105 3 0.308624756198725 1105 4 0.35034702169777177 1105 5 0.2906686760910091 1105 6 0.32480956839849284 1105 7 0.34814487206007527 1105 8 0.311015681748664 1105 9 0.41658051777331195 1105 10-11 0.4194405402025225 1105 12-13 0.4021418060115991 1105 14-15 0.5780742669255758 1105 16-17 0.6163215808925173 1105 18-19 0.5096869201171614 1105 20-21 0.49212847893359424 1105 22-23 0.5489068257717804 1105 24-25 0.42954821286469524 1105 26-27 0.3317896375927134 1105 28-29 0.3840359516265224 1105 30-31 0.2302547803150219 1105 32-33 0.39132247833559575 1105 34-35 0.23351085278354589 1105 36-37 0.15549548714763972 1105 38-39 0.032327093588953915 1105 40-41 0.23738924017795426 1105 42-43 0.19494996885135407 1105 44-45 0.24753262412600208 1105 46-47 0.37021740423077887 1105 48-49 0.45352971966948274 1105 50-51 0.5295145874113132 1105 52-53 0.6145981808727257 1105 54-55 0.49249597407118983 1105 56-57 0.5165267837928198 1105 58-59 0.6303794579390924 1105 60-61 0.43218183050783665 1105 62-63 0.4600887161817866 1105 64-65 0.5940898604771547 1105 66-67 0.4270198005811352 1105 68-69 0.5786210921967481 1105 70-71 0.6413474544130153 1105 72-73 0.5886431275952972 1105 74-75 0.5487786293282646 1105 76-77 0.707218291231527 1105 78-79 0.6909819530850427 1105 80-81 0.684526419193503 1105 82-83 0.5495851286742024 1105 84-85 0.6466799519478919 1105 86-87 0.6799906044614126 1105 88-89 0.5330449501375547 1105 90-91 0.6447535158754114 1105 92-93 0.6401053175313294 1105 94-95 0.64249942003784 1105 96-97 0.9330606887780206 1105 98-99 1.0921650645226428 1105 100-101 1.4574800039578655 1106 1 0.09677259713097541 1106 2 0.06248595333442708 1106 3 0.041909545842734985 1106 4 0.2615282514712334 1106 5 0.24373232226145092 1106 6 0.14493362449342584 1106 7 0.34884066494034016 1106 8 0.43662517473679685 1106 9 0.41241654797827465 1106 10-11 0.48923018421546516 1106 12-13 0.5144907812003794 1106 14-15 0.4436379130960191 1106 16-17 0.2280664568364159 1106 18-19 0.344252723784912 1106 20-21 0.22059665584837518 1106 22-23 0.05459452803715692 1106 24-25 -0.17774682489151417 1106 26-27 -0.20706419196499581 1106 28-29 -0.00367170748890544 1106 30-31 0.02506599930100606 1106 32-33 0.15858245676062666 1106 34-35 0.16376705558829485 1106 36-37 -0.02966632515009593 1106 38-39 0.017105950115386293 1106 40-41 0.17880509778313325 1106 42-43 0.08171911663992404 1106 44-45 -0.03748086023213659 1106 46-47 0.15759334813585468 1106 48-49 0.11018559884963253 1106 50-51 -0.06143740827369015 1106 52-53 -0.07348164652749034 1106 54-55 0.09144689100754277 1106 56-57 0.25340110957491646 1106 58-59 0.1545865776801989 1106 60-61 0.2169256277030982 1106 62-63 0.22692258888944394 1106 64-65 0.09166806975439101 1106 66-67 -0.03752442811357781 1106 68-69 0.10091343308132394 1106 70-71 -0.18248480017167168 1106 72-73 -0.07687736862909134 1106 74-75 0.1525596433519958 1106 76-77 -0.08612313271669336 1106 78-79 -0.05175267474667322 1106 80-81 0.2971990189777536 1106 82-83 0.3844664663225288 1106 84-85 0.5977182475250231 1106 86-87 0.6126443261442596 1106 88-89 0.6046118325539531 1106 90-91 0.5624018438150067 1106 92-93 0.6430314232487007 1106 94-95 0.6891094524002987 1106 96-97 0.6665126844630223 1106 98-99 0.9143468336704288 1106 100-101 1.284529626395834 1107 1 0.20698891964818245 1107 2 0.19395104674642738 1107 3 0.3097512370915041 1107 4 0.23728783849188062 1107 5 0.21548502629291733 1107 6 0.08251474543737913 1107 7 0.171067803583405 1107 8 0.2103567381586302 1107 9 0.2794249058937055 1107 10-11 0.3975991615999277 1107 12-13 0.29198832298995825 1107 14-15 0.22809341948736517 1107 16-17 0.34348558171350874 1107 18-19 0.49316619477802703 1107 20-21 0.39301307669400387 1107 22-23 0.30127225665072643 1107 24-25 0.19497318494133253 1107 26-27 0.16460984933294043 1107 28-29 0.13752667009222108 1107 30-31 0.03837475053011019 1107 32-33 0.22658270258166624 1107 34-35 0.3294037320877976 1107 36-37 0.40649517927467826 1107 38-39 0.44593830016454916 1107 40-41 0.3364267791990656 1107 42-43 0.3593272778985295 1107 44-45 0.4218185497973579 1107 46-47 0.3899827286668298 1107 48-49 0.5934439567650642 1107 50-51 0.5162727687174566 1107 52-53 0.4098393957537283 1107 54-55 0.4222027907125536 1107 56-57 0.6044507654254616 1107 58-59 0.6534287605710531 1107 60-61 0.5097771517935499 1107 62-63 0.51814186125916 1107 64-65 0.5338583352411206 1107 66-67 0.5625566928303698 1107 68-69 0.5903775432091507 1107 70-71 0.6277831447644147 1107 72-73 0.6868578821083773 1107 74-75 0.684155021916407 1107 76-77 0.6713830128093647 1107 78-79 0.5939909339062268 1107 80-81 0.561956885251103 1107 82-83 0.5940805272861454 1107 84-85 0.6090383065220735 1107 86-87 0.6899538148364925 1107 88-89 0.7042566211478558 1107 90-91 0.6842209195278848 1107 92-93 0.7948013347531244 1107 94-95 0.7570347228034429 1107 96-97 0.9738344642073713 1107 98-99 0.9498925563842953 1107 100-101 1.6634443264941616 1108 1 0.12432508048991053 1108 2 0.11691503679577053 1108 3 0.16629766668247115 1108 4 0.24172026376002265 1108 5 0.20200933199006954 1108 6 0.24218400851800226 1108 7 0.2562753807621476 1108 8 0.26597233295492373 1108 9 -0.0814825815660214 1108 10-11 0.12537713762048242 1108 12-13 0.1062393731102631 1108 14-15 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-3.6667647736711544 2316 60-61 -3.7223911891137256 2316 62-63 -3.314053387086531 2316 64-65 -4.076485083398758 2316 66-67 -3.621555959645111 2316 68-69 -3.864206687038795 2316 70-71 -3.2805103256972004 2316 72-73 -2.354017008268727 2316 74-75 -2.856884801092445 2316 76-77 -3.1057252348187916 2316 78-79 -3.532278200272195 2316 80-81 -4.085090770812037 2316 82-83 -3.5472602604041974 2316 84-85 -3.410952923646146 2316 86-87 -3.9078661843662488 2316 88-89 -5.273188467746348 2316 90-91 -4.678108666695504 2316 92-93 -4.1309550632377885 2316 94-95 -3.688495652704809 2316 96-97 -5.3899137419634044 2316 98-99 -7.28351352668993 2316 100-101 -7.916896800030592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3134.0 3 1028.0 4 1082.0 5 1132.0 6 1203.0 7 1360.0 8 1730.0 9 2122.0 10 2754.0 11 3441.0 12 4671.0 13 6055.0 14 7146.0 15 9236.0 16 11656.0 17 15393.0 18 12236.0 19 8520.0 20 8387.0 21 8511.0 22 9282.0 23 10597.0 24 12207.0 25 13980.0 26 16599.0 27 20006.0 28 24400.0 29 30483.0 30 38207.0 31 49133.0 32 64865.0 33 87144.0 34 122029.0 35 174707.0 36 247489.0 37 313410.0 38 224023.0 39 30820.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.9625277875918 17.83264600405832 13.953294959620058 23.251531248729822 2 15.991003802381194 19.39195655421628 39.254163210477394 25.362876432925134 3 20.310328103281034 23.079918299182992 29.18341683416834 27.42633676336763 4 12.982498906181636 15.599537471091942 36.22095130945684 35.197012313269575 5 14.054486528382437 36.996521538024 33.59312933440427 15.355862599189296 6 33.11438396110419 34.75469148204013 16.64473630748593 15.48618824936975 7 29.43230567194047 32.32868488524795 19.702832843474454 18.536176599337125 8 27.22752219925496 33.2379837661891 19.10080043616956 20.433693598386373 9 26.493185719243506 16.54936953442783 20.194407881366796 36.763036864961876 10-11 25.876785192864293 25.553182349936254 26.195761118513484 22.37427133868597 12-13 26.921100520553647 23.0186360331649 26.916974599957616 23.143288846323838 14-15 23.41011373276395 24.509119938585684 25.71805802227644 26.36270830637392 16-17 22.65013893460649 27.75081611772727 26.44724720669387 23.151797740972377 18-19 22.46616441700455 26.30783980234274 28.70744118686882 22.518554593783886 20-21 23.265074605409342 25.223738098995728 28.67547857936425 22.83570871623068 22-23 22.747277094909567 25.181383776101313 28.958935094342685 23.112404034646435 24-25 23.019860694800855 25.307267441042814 28.912157467941896 22.76071439621444 26-27 22.48371618261878 25.38206534529256 29.286149824558883 22.848068647529775 28-29 22.19297047768116 26.23895026200015 28.95113382365854 22.616945436660146 30-31 22.35556845689403 25.551524408058224 28.834189435741074 23.258717699306672 32-33 22.175636770556356 25.680941147647896 28.989126253518254 23.15429582827749 34-35 23.22141174573552 25.642351747073004 28.48347425874318 22.652762248448298 36-37 23.034623351568428 25.2394274302638 28.87228110150523 22.853668116662536 38-39 22.67719758757539 25.637417580700607 28.791287772257117 22.89409705946689 40-41 22.837304178871474 25.769926149334953 28.771574149704925 22.62119552208864 42-43 22.513054977139728 25.92665965068406 28.152036426448422 23.40824894572779 44-45 22.7055096390327 25.837144607831647 28.187214427818258 23.270131325317394 46-47 23.22364903775098 26.280954653013232 27.743130709696732 22.752265599539054 48-49 23.156354119243574 26.175579786880025 27.502275527386477 23.165790566489928 50-51 22.672714757046812 26.525794530534014 27.87358503713189 22.92790567528729 52-53 23.263838439134865 26.542200158168765 27.224900550841006 22.969060851855357 54-55 22.63669101277194 26.32732101706831 27.58279889075499 23.45318907940476 56-57 23.08954948249404 26.463189004119364 27.113543835440513 23.333717677946087 58-59 23.529604762584764 26.053134742314654 27.106167930983517 23.311092564117068 60-61 22.908443906702463 26.243971885621363 27.951451981477483 22.89613222619869 62-63 22.757413263898414 26.395747163322564 27.899850983232565 22.946988589546454 64-65 22.482206700367023 26.804655223377853 27.523948853285972 23.18918922296915 66-67 22.557680067517214 26.607306844929447 27.228047765742012 23.60696532181133 68-69 22.69820274595495 26.401295390296635 27.57616464355675 23.324337220191666 70-71 22.983306882696347 26.366957358073506 27.224970401221636 23.42476535800851 72-73 22.904332531614553 26.333024146569528 27.548165630765997 23.214477691049918 74-75 22.612141631007244 27.212621201150395 27.062712561981456 23.112524605860905 76-77 22.831260008775843 26.644314515272093 27.088513519426243 23.435911956525814 78-79 22.935719893193593 26.725572284337062 27.111407934476066 23.22729988799328 80-81 23.2165625 26.8066875 26.89378125 23.08296875 82-83 22.90696231940032 26.711675776918053 27.25619726764328 23.12516463603835 84-85 22.85705715014224 27.039451646610036 26.929429748471378 23.174061454776343 86-87 22.88029486876234 27.068444936614526 26.922772868749217 23.128487325873913 88-89 22.730168749013767 27.13157923516904 26.41827445110872 23.719977564708476 90-91 22.84096597730487 26.879610319240488 26.644121946478894 23.63530175697575 92-93 23.110179737648096 26.933553913013313 26.894273313598244 23.061993035740347 94-95 22.79504825309484 26.646452095967437 27.002108123682422 23.556391527255297 96-97 22.651325972358375 26.527822091612297 27.269409120979844 23.55144281504949 98-99 22.659624339781857 26.869018970528487 26.549457761665156 23.9218989280245 100-101 22.68213638352397 26.436371023188947 26.8887430464404 23.992749546846678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 215.0 1 166.0 2 206.0 3 357.0 4 321.0 5 288.5 6 559.5 7 1100.5 8 1749.0 9 2093.5 10 1830.0 11 1479.5 12 1429.0 13 1493.0 14 1653.0 15 1831.0 16 1956.5 17 2081.5 18 2210.5 19 2415.0 20 2635.0 21 2942.5 22 3543.5 23 4329.0 24 5221.5 25 6442.5 26 8058.0 27 10122.0 28 11924.0 29 13906.5 30 16660.0 31 19903.5 32 23898.0 33 25905.0 34 26835.0 35 28739.0 36 31650.5 37 34622.0 38 36275.0 39 37150.5 40 38528.0 41 41525.5 42 43691.0 43 45890.5 44 48975.5 45 52948.0 46 55337.5 47 59830.5 48 71772.0 49 71670.0 50 61071.5 51 56633.0 52 55355.5 53 56221.0 54 56768.0 55 54078.5 56 50282.5 57 46696.0 58 44407.5 59 40532.0 60 34247.0 61 29177.0 62 24764.0 63 19489.0 64 14346.5 65 10910.0 66 8455.5 67 6899.0 68 5349.5 69 3897.5 70 3100.0 71 2484.5 72 2025.0 73 1752.5 74 1408.5 75 1096.5 76 814.0 77 536.5 78 357.5 79 240.0 80 157.5 81 112.5 82 81.0 83 55.5 84 36.0 85 19.0 86 14.5 87 9.0 88 7.5 89 8.5 90 10.0 91 7.0 92 3.5 93 5.0 94 4.0 95 2.0 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06280504190127423 2 0.025184509339515933 3 0.012873471275286059 4 0.018122847911810472 5 0.021559939757153836 6 0.02680931639367825 7 0.06811691111680487 8 0.050743974153069325 9 0.060930264531086936 10-11 0.053306169892325284 12-13 0.03418344071641492 14-15 0.03518332198051481 16-17 0.04352608127784826 18-19 0.04083890038057981 20-21 0.01702922777920122 22-23 0.012404776932739236 24-25 0.020091364150507128 26-27 0.02284103762678182 28-29 0.005968041295096207 30-31 7.186646585717946E-4 32-33 0.008967685087395871 34-35 0.010686231010067555 36-37 0.004405726819940132 38-39 0.00874896106087402 40-41 0.004311987951430767 42-43 0.002124747686212262 44-45 2.187240265218505E-4 46-47 4.062017635405796E-4 48-49 7.186646585717946E-4 50-51 9.686349745967666E-4 52-53 3.12462895031215E-5 54-55 1.5623144751560753E-4 56-57 0.003968278766896431 58-59 0.0031558752398152716 60-61 0.0052181303470212916 62-63 0.0021559939757153836 64-65 5.624332110561872E-4 66-67 3.437091845343366E-4 68-69 0.0034058455558402443 70-71 0.0024684568707465985 72-73 0.018372818227835444 74-75 0.01346715077584537 76-77 0.021497447178147593 78-79 0.0178728775957855 80-81 0.011873590011186172 82-83 0.00434323424093389 84-85 0.0033745992663371223 86-87 0.0012810978696279817 88-89 0.002031008817702898 90-91 0.006936676269692974 92-93 0.010217536667520732 94-95 0.011811097432179929 96-97 0.010967447615595647 98-99 0.02124747686212262 100-101 0.018122847911810472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1600190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.0099495132869 #Duplication Level Percentage of deduplicated Percentage of total 1 79.76818933420194 46.27348636043365 2 11.975810071774522 13.894322752887057 3 3.5119191313330465 6.1117875451012935 4 1.5035774818939982 3.488898152559435 5 0.8057272382062546 2.3370098204912457 6 0.49821982940242393 1.7341024290091815 7 0.336602395657249 1.3668401584689904 8 0.23957440813464362 1.1118159456453014 9 0.18356032474609327 0.9583492654047078 >10 1.058297523228008 11.31687466535305 >50 0.0741952100842145 2.9576246966324615 >100 0.038705347135945006 4.0751246200676094 >500 0.003567619974186157 1.4340897315146162 >1k 0.0017297551389993488 1.8782329719303468 >5k 3.243290885623779E-4 1.0614408845010224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6161 0.38501677925746314 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5420 0.3387097782138371 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5344 0.33396034220936266 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4727 0.29540242096251074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3164 0.19772651997575288 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3142 0.1963516832376155 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2877 0.17979114980096114 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1797 0.11229916447421867 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.249257900624301E-5 0.0 14-15 0.0 0.0 0.0 6.249257900624301E-5 0.0 16-17 0.0 0.0 0.0 6.249257900624301E-5 0.0 18-19 0.0 0.0 0.0 6.249257900624301E-5 0.0 20-21 0.0 0.0 0.0 6.249257900624301E-5 0.0 22-23 0.0 0.0 0.0 6.249257900624301E-5 0.0 24-25 0.0 0.0 0.0 1.2498515801248603E-4 0.0 26-27 0.0 0.0 0.0 3.1246289503121505E-4 0.0 28-29 0.0 0.0 0.0 7.186646585717947E-4 0.0 30-31 0.0 0.0 0.0 0.0016560533436654399 0.0 32-33 0.0 0.0 0.0 0.004124510214412038 0.0 34-35 3.124628950312151E-5 0.0 0.0 0.0071554002962148245 0.0 36-37 1.2498515801248603E-4 0.0 0.0 0.013560889644354732 0.0 38-39 1.8747773701872903E-4 0.0 0.0 0.02277854504777558 0.0 40-41 1.8747773701872903E-4 0.0 0.0 0.03243364850424012 0.0 42-43 1.8747773701872903E-4 0.0 0.0 0.04302614064579831 0.0 44-45 1.8747773701872903E-4 0.0 0.0 0.05624332110561871 0.0 46-47 1.8747773701872903E-4 0.0 0.0 0.07492860222848537 0.0 48-49 1.8747773701872903E-4 0.0 0.0 0.09511370524750186 0.0 50-51 1.8747773701872903E-4 0.0 0.0 0.11811097432179929 0.0 52-53 1.8747773701872903E-4 0.0 0.0 0.14232684868671847 0.0 54-55 1.8747773701872903E-4 0.0 0.0 0.17226079403070885 0.0 56-57 2.4997031602497205E-4 0.0 0.0 0.2092564008024047 3.124628950312151E-5 58-59 2.4997031602497205E-4 0.0 0.0 0.2549384760559683 6.249257900624301E-5 60-61 2.4997031602497205E-4 0.0 0.0 0.30583868165655326 6.249257900624301E-5 62-63 2.4997031602497205E-4 0.0 0.0 0.3589573738118598 6.249257900624301E-5 64-65 2.4997031602497205E-4 0.0 0.0 0.4106074903605197 6.249257900624301E-5 66-67 3.1246289503121505E-4 0.0 0.0 0.48038045482099 6.249257900624301E-5 68-69 3.1246289503121505E-4 0.0 0.0 0.563183122004262 6.249257900624301E-5 70-71 3.1246289503121505E-4 0.0 0.0 0.6516101212980958 6.249257900624301E-5 72-73 3.1246289503121505E-4 0.0 0.0 0.7474737374936726 6.249257900624301E-5 74-75 3.1246289503121505E-4 0.0 0.0 0.8497115967478862 6.249257900624301E-5 76-77 3.1246289503121505E-4 0.0 0.0 0.9665414732000575 6.249257900624301E-5 78-79 3.1246289503121505E-4 0.0 0.0 1.1035564526712454 6.249257900624301E-5 80-81 4.686943425468226E-4 0.0 0.0 1.2648185527968554 6.249257900624301E-5 82-83 4.999406320499441E-4 0.0 0.0 1.4257994363169373 6.249257900624301E-5 84-85 4.999406320499441E-4 0.0 0.0 1.591342278104475 6.249257900624301E-5 86-87 4.999406320499441E-4 0.0 0.0 1.7709147038789144 6.249257900624301E-5 88-89 4.999406320499441E-4 0.0 0.0 1.9725782563320606 6.249257900624301E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGT 35 0.008403621 27.13633 42-43 GTACATG 7140 0.0 24.69164 1 TACATGG 7250 0.0 23.595284 2 GAGTACT 5845 0.0 23.17082 12-13 ACATGGG 7225 0.0 22.889128 3 CTATCCG 105 0.002189699 22.631289 4 GTAAGGT 620 0.0 22.229767 4 TAAGGTG 775 0.0 22.076458 5 GTACTTT 6120 0.0 21.97229 14-15 GTACCTG 1230 0.0 21.635014 1 CATGGGG 3750 0.0 20.784575 4 TAGCCCT 965 0.0 20.192268 4 TACCTGG 1235 0.0 20.007696 2 GGTAAGG 690 0.0 19.972698 3 TCGCGCT 245 0.0 19.382488 60-61 GTATAGC 540 0.0 19.359903 1 AGTACTT 6130 0.0 19.264023 12-13 ATACCGT 150 7.6931936E-4 19.010878 6 TACCGTC 125 0.006012244 19.010878 7 GTATCAA 13755 0.0 18.9319 1 >>END_MODULE