FastQCFastQC Report
Thu 26 May 2016
SRR936376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600190
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT83030.5188758834888357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63730.3982652060067867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62150.3883913785238003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39340.24584580581055998No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24650.15404420725038903No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20210.12629750217161712No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19970.12479768027546728No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19940.12461020253844855No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19670.12292290290527999No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.12054818490304275No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17510.1094245058399315No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16580.1036126959923509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG700.00838987227.1446251
TAAGGTG8200.025.4830935
GGTAAGG7500.024.6954333
GTAAGGT7750.023.8988084
AGGTAAG7450.023.5877172
GAGTACT76800.020.77805912-13
GTACATG71900.020.0186881
GTACTTT78500.019.93483514-15
AGTACTT78550.019.7105312-13
TACATGG71900.019.6846562
TAGACCG1507.732471E-418.9964895
ACATGGG73600.018.3254173
AAGGTGT12050.018.1294296
AGAGTAC127150.017.64840910-11
TACTTTT87750.017.64401414-15
ACTTTTT91100.017.3079916-17
GTGTAAG6950.017.0874421
CATGGGG37250.016.9565964
GGTGTGC15350.016.7070088
GTACCTG13500.016.5381151
Produced by FastQC (version 0.11.5)