##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936376_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1600190 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.296159206094277 31.0 31.0 34.0 30.0 34.0 2 31.46856873246302 31.0 31.0 34.0 30.0 34.0 3 31.515384423099757 31.0 31.0 34.0 30.0 34.0 4 35.30957323817797 37.0 35.0 37.0 33.0 37.0 5 35.10270217911623 37.0 35.0 37.0 32.0 37.0 6 35.220757535042715 37.0 35.0 37.0 32.0 37.0 7 35.14926539973378 37.0 35.0 37.0 32.0 37.0 8 35.10986382867034 37.0 35.0 37.0 32.0 37.0 9 36.62012448521738 38.0 35.0 39.0 32.0 39.0 10-11 36.46791224792056 38.0 35.0 39.0 32.0 39.0 12-13 36.333792862097624 38.0 35.0 39.0 32.0 39.0 14-15 37.246510726851184 39.0 36.0 40.0 32.0 41.0 16-17 37.10101019253963 38.5 36.0 40.0 31.0 41.0 18-19 37.283575075459794 39.0 36.0 40.0 32.0 41.0 20-21 37.296871309032056 39.0 36.0 40.0 31.5 41.0 22-23 37.17912279166849 39.0 36.0 40.0 31.0 41.0 24-25 36.99362825664453 39.0 36.0 40.0 31.0 41.0 26-27 36.69263087508358 38.5 35.5 40.0 30.0 41.0 28-29 36.5660796530412 38.5 35.0 40.0 30.0 41.0 30-31 36.49470000437448 38.0 35.0 40.0 30.0 41.0 32-33 36.2722107999675 38.0 35.0 40.0 30.0 41.0 34-35 36.03708590854836 38.0 35.0 40.0 30.0 41.0 36-37 35.750113111568 38.0 34.5 40.0 29.0 41.0 38-39 35.50954980346084 38.0 34.0 40.0 27.5 41.0 40-41 35.25463163749305 38.0 34.0 40.0 27.0 41.0 42-43 34.88602540948263 38.0 33.0 40.0 26.0 41.0 44-45 34.53040576431549 38.0 33.0 40.0 24.5 41.0 46-47 34.06420706291128 37.5 33.0 40.0 23.5 41.0 48-49 33.57053099944382 37.0 32.5 40.0 22.5 41.0 50-51 33.17418275329805 37.0 32.0 40.0 20.5 41.0 52-53 32.74190846087027 36.5 31.0 39.0 19.0 40.0 54-55 32.46613214680757 36.0 31.0 39.0 18.0 40.0 56-57 32.041085433604756 36.0 30.5 39.0 16.0 40.0 58-59 31.563286859685412 35.0 30.0 39.0 13.5 40.0 60-61 31.028547859941632 35.0 29.0 38.0 8.5 40.0 62-63 30.600159043613573 34.0 28.5 38.0 8.0 40.0 64-65 30.079801773539394 34.0 28.0 37.5 7.0 39.5 66-67 29.552832163680563 34.0 27.5 37.0 6.5 39.0 68-69 29.091429142789295 33.5 26.5 36.0 2.0 39.0 70-71 28.60502190364894 33.0 26.0 36.0 2.0 39.0 72-73 28.036919990751098 32.5 26.0 35.5 2.0 38.0 74-75 27.449859391697238 32.0 25.0 35.0 2.0 37.5 76-77 26.268094101325467 30.5 24.0 34.0 2.0 36.0 78-79 26.986662833788486 32.0 25.0 34.5 2.0 36.0 80-81 26.711667676963362 32.0 25.0 35.0 2.0 36.0 82-83 26.258031858716777 32.0 24.0 34.0 2.0 36.0 84-85 25.76423018516551 31.0 22.5 34.0 2.0 35.0 86-87 25.228241333841606 31.0 20.0 34.0 2.0 35.0 88-89 24.551377023978404 31.0 18.5 34.0 2.0 35.0 90-91 23.8502452833726 30.0 13.5 34.0 2.0 35.0 92-93 22.976538411063686 30.0 4.5 34.0 2.0 35.0 94-95 22.116522100500568 29.0 2.0 34.0 2.0 35.0 96-97 21.043534517776017 29.0 2.0 34.0 2.0 35.0 98-99 19.780299214468283 28.0 2.0 33.0 2.0 34.0 100-101 16.599970003562078 21.0 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31958639556386714 1101 2 0.2060349132970849 1101 3 0.339130479378678 1101 4 0.3571532606395422 1101 5 0.27001744331780486 1101 6 0.13154028393542205 1101 7 0.21496593852812396 1101 8 0.19474415919062693 1101 9 0.22218372563132505 1101 10-11 0.38376218730847 1101 12-13 0.40508856687155514 1101 14-15 0.4069426762160049 1101 16-17 0.41844266600930524 1101 18-19 0.434260403074866 1101 20-21 0.4156687768773395 1101 22-23 0.40377094991185913 1101 24-25 0.32573321923273113 1101 26-27 0.2732207291433113 1101 28-29 0.32866987619475196 1101 30-31 0.4696887106675689 1101 32-33 0.4162511496280885 1101 34-35 0.42669320518895404 1101 36-37 0.3987946926917161 1101 38-39 0.42639417641472477 1101 40-41 0.5129360614239005 1101 42-43 0.45754920602411886 1101 44-45 0.5280905681754078 1101 46-47 0.5741122703473636 1101 48-49 0.4943077929201891 1101 50-51 0.5454219182660793 1101 52-53 0.6344685951138374 1101 54-55 0.4814418432969845 1101 56-57 0.44288188277230134 1101 58-59 0.16113892156133147 1101 60-61 0.49214280121144327 1101 62-63 0.46419969106522885 1101 64-65 0.6854091505996962 1101 66-67 0.4819609823088449 1101 68-69 0.348629785163002 1101 70-71 0.3229176428457272 1101 72-73 0.42578882417562625 1101 74-75 0.4793638349211058 1101 76-77 0.31818215938256245 1101 78-79 0.38242103891519363 1101 80-81 0.4100246168325157 1101 82-83 0.7666762532669438 1101 84-85 0.6723251395936813 1101 86-87 0.6803018628170179 1101 88-89 0.9093643273245782 1101 90-91 1.0866851257715417 1101 92-93 0.9563920828166239 1101 94-95 0.9630491064729192 1101 96-97 1.0774864787453708 1101 98-99 1.0995815136316018 1101 100-101 1.5298517395394313 1102 1 0.2563277186787367 1102 2 0.1916156479693889 1102 3 0.2642086594350559 1102 4 0.2447556655021188 1102 5 0.16472542429031733 1102 6 0.1857620069798216 1102 7 0.1410164142152297 1102 8 0.21488708554721825 1102 9 0.24578419250941153 1102 10-11 0.2953179704303963 1102 12-13 0.3368990407968582 1102 14-15 0.43649859147323156 1102 16-17 0.2257431416964053 1102 18-19 0.1963656488465375 1102 20-21 0.18239600555951085 1102 22-23 0.3633876474101001 1102 24-25 0.2182103157795936 1102 26-27 0.16206410475994204 1102 28-29 0.32226902171976946 1102 30-31 0.4318071699698933 1102 32-33 0.4949316684793885 1102 34-35 0.44967373725378224 1102 36-37 0.3814656978009481 1102 38-39 0.42528093645700693 1102 40-41 0.5963090353492078 1102 42-43 0.5868821429515378 1102 44-45 0.7672378549971128 1102 46-47 0.6809612837370693 1102 48-49 1.0201468521168096 1102 50-51 0.9624921481815107 1102 52-53 1.0590044698495689 1102 54-55 0.9027183804710504 1102 56-57 0.8716648064861836 1102 58-59 1.130787992209676 1102 60-61 1.1474871237977737 1102 62-63 1.0556191545958846 1102 64-65 0.9170610621571278 1102 66-67 0.8624792830488026 1102 68-69 0.9381299833659611 1102 70-71 1.0412371019718734 1102 72-73 1.0490083009198088 1102 74-75 0.9575405879161707 1102 76-77 0.8607539528991239 1102 78-79 0.9675277381752352 1102 80-81 1.1190472560150369 1102 82-83 1.115124764542493 1102 84-85 1.1475525224337098 1102 86-87 1.1821490042898901 1102 88-89 0.9323349492414224 1102 90-91 1.0235095840167823 1102 92-93 1.1348909156214155 1102 94-95 0.9593531718358506 1102 96-97 1.3893467236361374 1102 98-99 1.538166674836674 1102 100-101 1.7604135348177934 1103 1 0.31989720351921136 1103 2 0.35761164876262086 1103 3 0.315765100030859 1103 4 0.34162488152538373 1103 5 0.38917512021840395 1103 6 0.3194396246424276 1103 7 0.352100166198305 1103 8 0.24300519986063307 1103 9 0.4219776197084215 1103 10-11 0.4484447607089024 1103 12-13 0.436127017616883 1103 14-15 0.4939856739251951 1103 16-17 0.5405716054721879 1103 18-19 0.5747521672230604 1103 20-21 0.38931997656250417 1103 22-23 0.3905699969165326 1103 24-25 0.5707178074066022 1103 26-27 0.3540910305487657 1103 28-29 0.3613772426786994 1103 30-31 0.560858019388192 1103 32-33 0.41314307007466766 1103 34-35 0.41552899461320436 1103 36-37 0.196563667349686 1103 38-39 0.33219113608683415 1103 40-41 0.46280053014913847 1103 42-43 0.44130082574723417 1103 44-45 0.5505623746516122 1103 46-47 0.7301493235748993 1103 48-49 0.6339143693034828 1103 50-51 0.5661257249927232 1103 52-53 0.59470110853281 1103 54-55 0.680725084375517 1103 56-57 0.4851259106197965 1103 58-59 0.6990208771470989 1103 60-61 0.8591111267503493 1103 62-63 0.8015773942838784 1103 64-65 0.638697817401269 1103 66-67 0.48114706795097106 1103 68-69 0.6542156357985363 1103 70-71 0.7057050311980291 1103 72-73 0.8934631197987102 1103 74-75 0.6984826049265713 1103 76-77 0.7010627621711123 1103 78-79 0.8016643557056433 1103 80-81 0.693230338158525 1103 82-83 0.8732143445706981 1103 84-85 1.2996965875227247 1103 86-87 1.1696301375086406 1103 88-89 1.139068875294658 1103 90-91 1.314791891658242 1103 92-93 1.3330971942658714 1103 94-95 1.4255046514321847 1103 96-97 1.6668803808721684 1103 98-99 1.5082639282740082 1103 100-101 1.6766150044987427 1104 1 0.22283476622203935 1104 2 0.19266780862686517 1104 3 0.1964456468864526 1104 4 0.17996079540667864 1104 5 0.32355031824577907 1104 6 0.1341415138727129 1104 7 0.1650852106097105 1104 8 0.14997733386817913 1104 9 0.2507423270092346 1104 10-11 0.3004580928234617 1104 12-13 0.21668043439883888 1104 14-15 0.290606436559834 1104 16-17 0.39653118517467334 1104 18-19 0.3878762864393295 1104 20-21 0.38679630160051204 1104 22-23 0.38427041296330344 1104 24-25 0.3080212030189031 1104 26-27 0.3578201139504742 1104 28-29 0.4342360374497076 1104 30-31 0.4714951670536678 1104 32-33 0.46807285131784937 1104 34-35 0.20284955026191653 1104 36-37 0.31925428254820787 1104 38-39 0.3419150165206304 1104 40-41 0.6421313223764216 1104 42-43 0.41414829265107045 1104 44-45 0.4082417432968626 1104 46-47 0.48922171145004256 1104 48-49 0.5136483102292146 1104 50-51 0.5024237163151284 1104 52-53 0.5418376816735346 1104 54-55 0.4833334940270859 1104 56-57 0.29007209483895124 1104 58-59 0.231557091272947 1104 60-61 0.3535545835892151 1104 62-63 0.43497775083130463 1104 64-65 0.6101245342320674 1104 66-67 0.4266933936002353 1104 68-69 0.4356801888272521 1104 70-71 0.417353331204815 1104 72-73 0.6157318560629719 1104 74-75 0.5419945690048955 1104 76-77 0.5306229437085968 1104 78-79 0.45651153324416427 1104 80-81 0.7566588237068004 1104 82-83 0.60775853530955 1104 84-85 0.9250592054489104 1104 86-87 1.0638748239258007 1104 88-89 1.0385943386016194 1104 90-91 1.2325490524685314 1104 92-93 1.3167294075797038 1104 94-95 1.2644916372830046 1104 96-97 1.356788288676789 1104 98-99 1.2872329858122846 1104 100-101 1.6959047640507379 1105 1 0.09322935028607304 1105 2 0.20441736048287495 1105 3 0.2226313266410962 1105 4 0.29683057111161304 1105 5 0.25245128728924016 1105 6 0.21572087968748122 1105 7 -0.02764930854637271 1105 8 0.2222208908330856 1105 9 0.07266357492580511 1105 10-11 0.2448519173020216 1105 12-13 0.18818511145489936 1105 14-15 0.3140277513977239 1105 16-17 0.3736429529153966 1105 18-19 0.36438833627911293 1105 20-21 0.34912503738259204 1105 22-23 0.42745267572725254 1105 24-25 0.37595033735456695 1105 26-27 0.3083761328074104 1105 28-29 0.3158791215040253 1105 30-31 0.5488387570788476 1105 32-33 0.3845141590403429 1105 34-35 0.522034174146988 1105 36-37 0.45871000065962875 1105 38-39 0.38149554001687846 1105 40-41 0.614263458218673 1105 42-43 0.7209925124229457 1105 44-45 0.6687481031093014 1105 46-47 0.5099324272106429 1105 48-49 0.4580884648460426 1105 50-51 0.49241983831095837 1105 52-53 0.6379140909391054 1105 54-55 0.4531760058755836 1105 56-57 0.44049318431789075 1105 58-59 0.51503404803492 1105 60-61 0.840368225469831 1105 62-63 0.8605249326433473 1105 64-65 0.9955046301182939 1105 66-67 0.8028961182699454 1105 68-69 0.9806621772710322 1105 70-71 1.0552996019718748 1105 72-73 1.1936634384602627 1105 74-75 1.2336446197392554 1105 76-77 1.0746731815938375 1105 78-79 0.8384546192431976 1105 80-81 0.5581350391865598 1105 82-83 0.9417647996018239 1105 84-85 1.2220325318727596 1105 86-87 1.2696498470622721 1105 88-89 1.2314298117009663 1105 90-91 1.3983334445885198 1105 92-93 1.1695937540788073 1105 94-95 1.4450504817063994 1105 96-97 1.6479649140352741 1105 98-99 1.215871131417039 1105 100-101 1.4309384134585699 1106 1 0.14851521867873885 1106 2 0.12609481463605476 1106 3 0.13972949276838875 1106 4 0.2085403877243408 1106 5 0.18951709095698988 1106 6 0.0494425625353756 1106 7 -0.014747474673661998 1106 8 0.04589402999166481 1106 9 0.17193002584274097 1106 10-11 0.0548492204303912 1106 12-13 0.19832265190797216 1106 14-15 0.3486340081398964 1106 16-17 0.1845625861408493 1106 18-19 0.1985010655132058 1106 20-21 0.20958350555950744 1106 22-23 0.10232861963232409 1106 24-25 0.221543649112931 1106 26-27 0.03192521587105546 1106 28-29 -0.1526268116135654 1106 30-31 0.004359253303228172 1106 32-33 0.2141851407016091 1106 34-35 0.3515487372537862 1106 36-37 -0.20289194108794817 1106 38-39 0.1857323253458958 1106 40-41 0.27144792423808894 1106 42-43 0.391847420729313 1106 44-45 0.2747378549971131 1106 46-47 0.3905446170704039 1106 48-49 0.24410518545014526 1106 50-51 0.4300789537370733 1106 52-53 0.288952386516236 1106 54-55 0.344888519359948 1106 56-57 0.11470300093062136 1106 58-59 0.29136090887634225 1106 60-61 0.2461503182422149 1106 62-63 0.38046290459588405 1106 64-65 0.42876245104601907 1106 66-67 0.5371841441599123 1106 68-69 0.3007862333659581 1106 70-71 0.3569662686385371 1106 72-73 0.6648069120309259 1106 74-75 0.5910475323606157 1106 76-77 0.5481845084546819 1106 78-79 0.48858676595301276 1106 80-81 0.626338922681704 1106 82-83 0.8950900423202697 1106 84-85 1.2765282168781553 1106 86-87 1.4819233098454418 1106 88-89 1.412508560352535 1106 90-91 1.4148463895723395 1106 92-93 1.473970776732525 1106 94-95 1.5098913662802964 1106 96-97 1.6786175569694706 1106 98-99 1.6516909803922282 1106 100-101 1.9021843681511292 1107 1 -0.04746560727996396 1107 2 -0.10035061309255866 1107 3 -0.14341947478323291 1107 4 0.018884537183531336 1107 5 -0.09586638986897356 1107 6 -0.11697582487858682 1107 7 -0.05829221410336061 1107 8 -0.014339499998506255 1107 9 -0.13453015114841094 1107 10-11 -0.058196502283472284 1107 12-13 -0.18989391643027886 1107 14-15 0.019917193980603543 1107 16-17 0.053923451430925695 1107 18-19 -0.208287282569394 1107 20-21 -0.1771679693667494 1107 22-23 -0.2466118916754425 1107 24-25 -0.07480222109355594 1107 26-27 -0.13343737016631252 1107 28-29 -0.09009731308849211 1107 30-31 -0.16847629241949136 1107 32-33 0.07404993912835778 1107 34-35 0.06504062515939069 1107 36-37 -0.17321027933769528 1107 38-39 -0.11071187327750209 1107 40-41 -0.19100659886908744 1107 42-43 -0.07174156648799368 1107 44-45 0.09889345971687646 1107 46-47 -0.08459918823933066 1107 48-49 0.08704397601061231 1107 50-51 0.23191277871248417 1107 52-53 0.45619058710620664 1107 54-55 0.21371349477784207 1107 56-57 0.16313097536523813 1107 58-59 0.26281002392058994 1107 60-61 0.06250602129039606 1107 62-63 0.2354518426489811 1107 64-65 0.23694952085919496 1107 66-67 0.2544801126948215 1107 68-69 0.37544331301197786 1107 70-71 0.23966538368278734 1107 72-73 0.34628429649503545 1107 74-75 0.48970780768017974 1107 76-77 0.2622012242855547 1107 78-79 0.24236872268850718 1107 80-81 0.5198308105873082 1107 82-83 0.5409774562829028 1107 84-85 0.9752511765635035 1107 86-87 1.020379553699918 1107 88-89 1.0034955318372916 1107 90-91 0.9671558782645704 1107 92-93 1.103431199544719 1107 94-95 1.175874650449419 1107 96-97 1.552146318031415 1107 98-99 1.3292548938632223 1107 100-101 1.5500199138738395 1108 1 -0.012391079324334697 1108 2 0.04274612031962022 1108 3 -0.018987865142516824 1108 4 0.08767761250672379 1108 5 0.1329425901889394 1108 6 0.006859357210231565 1108 7 -0.058047525876936845 1108 8 0.08230980060098148 1108 9 -0.0016644442033424411 1108 10-11 -0.05828826036837853 1108 12-13 -0.034589534471962224 1108 14-15 0.03588055191870154 1108 16-17 0.03886238132774622 1108 18-19 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-3.261206917505671 2316 100-101 -3.79227509130833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 49.0 5 117.0 6 223.0 7 603.0 8 1375.0 9 2431.0 10 3735.0 11 5697.0 12 7671.0 13 10489.0 14 13479.0 15 16813.0 16 20160.0 17 23994.0 18 21918.0 19 18449.0 20 18176.0 21 19156.0 22 20816.0 23 23562.0 24 27005.0 25 31756.0 26 37500.0 27 44902.0 28 53523.0 29 62362.0 30 73565.0 31 86630.0 32 101988.0 33 120652.0 34 144731.0 35 174342.0 36 197212.0 37 163683.0 38 50289.0 39 1119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83836876134471 20.418798097838316 15.48762894846062 22.255204192356352 2 17.41883669840595 21.72842129605035 36.69834345303013 24.15439855251357 3 21.411148421255906 23.74256375066324 29.89792519347216 24.94836263460869 4 14.543585449227905 17.373624382104623 35.140639549053546 32.94215061961392 5 13.85662978056446 37.74999984376474 33.513337178803745 14.880033196867046 6 33.00208100288091 36.133959092357784 15.67938807266637 15.184571832094939 7 29.05498722026759 32.83422593567014 19.517994738124848 18.59279210593742 8 27.387310256906993 34.10682481455327 18.455683387597723 20.050181540942013 9 26.205325617582915 16.550597116592407 19.750904580081116 37.49317268574357 10-11 26.128334760247217 26.13302170367269 25.913891475387295 21.82475206069279 12-13 27.212418622309087 23.42071426451128 26.76856161269609 22.59830550048354 14-15 23.202153750299576 25.16714468416318 25.69592712844855 25.9347744370887 16-17 22.379762640538086 28.189357632129703 26.610137927457316 22.820741799874888 18-19 22.32412108584679 26.53945313952482 29.063746644498195 22.072679130130197 20-21 23.220555059086735 25.162012011073685 29.24602703428968 22.371405895549902 22-23 22.7428831040843 25.267335692013788 29.390568673628138 22.59921253027378 24-25 23.09747592473394 25.533499146976297 29.37060599053862 21.99841893775114 26-27 22.573480588750865 25.450960602561917 29.66320713300326 22.312351675683953 28-29 22.16196741678431 26.380980771165685 29.436727454755772 22.02032435729423 30-31 22.496352732411953 25.52777969597615 29.27250564861682 22.70336192299508 32-33 22.33033858068661 25.732363421373915 29.422218250189914 22.51507974774956 34-35 23.223184344624244 25.61271768054064 29.102293137087027 22.061804837748085 36-37 23.166090846569592 25.247760957116427 29.295957989765537 22.290190206548445 38-39 22.58652356529396 25.70569020435941 29.38576564629655 22.32202058405008 40-41 22.850165996172322 25.8041635745811 29.010975276334804 22.334695152911767 42-43 22.673766579683257 26.00287417111349 28.489010310831077 22.834348938372184 44-45 22.98991544018163 25.647228511488475 28.485147506294652 22.877708542035247 46-47 23.523680818654064 26.361254540483536 27.818021325625903 22.297043315236493 48-49 23.52035211082767 26.368753976120157 27.57765073635558 22.533243176696594 50-51 22.995127450569903 26.635041235754027 27.827587866183308 22.542243447492766 52-53 23.61629323600295 26.748305118375338 27.15119590822439 22.48420573739732 54-55 22.99154710585553 26.41545043564339 27.482592387520764 23.110410070980315 56-57 23.417651303426876 26.56667638568698 27.20053776495659 22.815134545929556 58-59 23.930211908943193 26.150406812789857 27.07573284277646 22.84364843549049 60-61 23.125426790488646 26.46533875249824 27.896792098145013 22.5124423588681 62-63 23.06050693964619 26.474907240651174 27.81055436230663 22.654031457396012 64-65 22.76319075002883 26.991124860422577 27.43161604794244 22.81406834160615 66-67 22.94983154693719 26.68126530716827 27.184437199285817 23.184465946608725 68-69 23.062864492067103 26.498463915129122 27.377369432785976 23.0613021600178 70-71 23.293402583573044 26.587923786407465 27.198039979077443 22.920633650942047 72-73 23.25459680075291 26.405037154986065 27.424947928026 22.91541811623502 74-75 22.914634980856714 27.403128231103018 26.83234494845702 22.849891839583243 76-77 23.240803118684234 26.72098200057244 26.871435517153884 23.166779363589438 78-79 23.22600402484907 26.845212054547957 26.976238391060335 22.952545529542643 80-81 23.52847240386722 26.82361419321652 26.818489069893243 22.829424333023013 82-83 23.39812873472554 26.858772567734846 26.989684258752007 22.753414438787612 84-85 23.102756254604202 27.081068021701988 26.87256819219615 22.943607531497666 86-87 23.258852414908603 27.04765755881376 26.512854919237427 23.180635107040214 88-89 22.97759866517839 27.30345342580978 26.280144372284695 23.43880353672713 90-91 23.22993648771235 26.934048875515543 26.442749227430756 23.393265409341353 92-93 23.367872230770217 27.106142960593903 26.5437255690667 22.98225923956918 94-95 23.151559552281164 26.797307586041352 26.670975662508113 23.38015719916937 96-97 22.840089892248717 26.70611930635795 26.972269885565254 23.48152091582808 98-99 22.921150634109193 27.05354376836766 26.222694885143223 23.802610712379924 100-101 22.878932256886607 26.850955646593338 26.24436015946618 24.025751937053876 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 344.0 1 266.5 2 271.0 3 371.5 4 311.5 5 277.0 6 514.0 7 1061.5 8 1606.0 9 1908.0 10 1797.5 11 1472.0 12 1423.5 13 1563.0 14 1717.0 15 1889.5 16 2104.5 17 2291.5 18 2501.5 19 2767.5 20 3101.0 21 3528.0 22 4069.5 23 4806.0 24 5665.5 25 6948.0 26 8784.5 27 10797.0 28 12635.0 29 14744.5 30 17477.0 31 20683.0 32 24505.0 33 26638.5 34 27644.5 35 29380.5 36 32333.5 37 35073.0 38 36469.5 39 37643.0 40 39158.0 41 41816.5 42 43814.5 43 45930.5 44 48631.0 45 52103.0 46 54647.5 47 59697.5 48 70832.0 49 70813.0 50 61066.5 51 56292.0 52 54839.5 53 55263.5 54 55918.5 55 53735.5 56 49815.0 57 45994.0 58 43060.5 59 39302.5 60 33532.0 61 28496.5 62 24162.0 63 18947.5 64 14159.0 65 10977.0 66 8441.5 67 6685.0 68 5144.0 69 3843.0 70 2968.5 71 2336.5 72 1938.0 73 1628.5 74 1325.0 75 1071.5 76 809.0 77 538.5 78 381.0 79 269.0 80 199.0 81 151.0 82 92.0 83 59.0 84 37.5 85 22.5 86 12.5 87 7.0 88 3.5 89 2.5 90 2.5 91 2.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02037258075603522 2 0.010936201326092526 3 0.0055618395315556275 4 0.0 5 0.0024372105812434774 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.811572375780376E-4 14-15 2.812166055280936E-4 16-17 6.2492579006243E-5 18-19 0.0012186052906217387 20-21 0.0 22-23 9.373886850936451E-5 24-25 0.0 26-27 0.0014060830276404676 28-29 0.0025621957392559635 30-31 0.0012186052906217387 32-33 0.0070929077172085814 34-35 0.0014998218961498322 36-37 5.311869215530655E-4 38-39 0.0011873590011186172 40-41 1.5623144751560753E-4 42-43 0.00478068229397759 44-45 0.001312344159131103 46-47 1.5623144751560753E-4 48-49 7.499109480749161E-4 50-51 6.2492579006243E-5 52-53 2.812166055280936E-4 54-55 0.001749792212174804 56-57 0.014654509776963986 58-59 0.02346596341684425 60-61 0.01952893093945094 62-63 0.013529643354851613 64-65 0.01696673520019498 66-67 0.0025621957392559635 68-69 0.0011248664221123743 70-71 1.8747773701872903E-4 72-73 6.2492579006243E-5 74-75 0.0012810978696279817 76-77 0.001749792212174804 78-79 0.008124035270811592 80-81 0.014279554302926527 82-83 0.016029346515101333 84-85 0.02677807010417513 86-87 0.010030058930502002 88-89 0.03596447921809285 90-91 0.04105762440710165 92-93 0.02496578531299408 94-95 0.027246764446721954 96-97 0.04496341059499184 98-99 0.04633824733312919 100-101 0.05336866247133153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1600190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.27772691508355 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1559668438131 46.71307546338232 2 11.785977774177955 13.737199883015741 3 3.4338514354164107 6.003511686604958 4 1.4429208113842396 3.3636058002376603 5 0.7771269972827356 2.264459746299107 6 0.48799885972893775 1.706367856929312 7 0.3360611525520984 1.3709416052639596 8 0.2387494957058134 1.1131022329485845 9 0.1750662702155921 0.9182217858899852 >10 1.0488390410726927 11.314729713590602 >50 0.07530307769043307 2.9976634855082405 >100 0.03687117702318956 4.027892140071314 >500 0.0031172419217885402 1.2761080934046247 >1k 0.0018273487127725926 1.8844378609689227 >5k 3.2247330225398696E-4 1.3086826458846408 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8303 0.5188758834888357 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6373 0.3982652060067867 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6215 0.3883913785238003 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3934 0.24584580581055998 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2465 0.15404420725038903 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2021 0.12629750217161712 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1997 0.12479768027546728 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1994 0.12461020253844855 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1967 0.12292290290527999 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1929 0.12054818490304275 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1751 0.1094245058399315 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1658 0.1036126959923509 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 6.249257900624301E-5 0.0 0.0 0.0 0.0 12-13 6.249257900624301E-5 0.0 0.0 6.249257900624301E-5 3.124628950312151E-5 14-15 6.249257900624301E-5 0.0 0.0 6.249257900624301E-5 6.249257900624301E-5 16-17 1.2498515801248603E-4 0.0 0.0 6.249257900624301E-5 6.249257900624301E-5 18-19 2.1872402652185053E-4 0.0 0.0 6.249257900624301E-5 6.249257900624301E-5 20-21 2.4997031602497205E-4 0.0 0.0 6.249257900624301E-5 6.249257900624301E-5 22-23 3.1246289503121505E-4 0.0 0.0 6.249257900624301E-5 6.249257900624301E-5 24-25 3.1246289503121505E-4 0.0 0.0 1.2498515801248603E-4 6.249257900624301E-5 26-27 3.7495547403745805E-4 0.0 0.0 3.1246289503121505E-4 6.249257900624301E-5 28-29 3.7495547403745805E-4 0.0 0.0 7.186646585717947E-4 6.249257900624301E-5 30-31 3.7495547403745805E-4 0.0 0.0 0.0015935607646591968 6.249257900624301E-5 32-33 3.7495547403745805E-4 0.0 0.0 0.0040307713459026735 6.249257900624301E-5 34-35 4.062017635405795E-4 0.0 0.0 0.0070929077172085814 6.249257900624301E-5 36-37 4.3744805304370105E-4 0.0 0.0 0.01352964335485161 6.249257900624301E-5 38-39 4.3744805304370105E-4 0.0 0.0 0.022684806179266215 6.249257900624301E-5 40-41 4.3744805304370105E-4 0.0 0.0 0.03224617076722139 6.249257900624301E-5 42-43 4.686943425468226E-4 0.0 0.0 0.04224498340822028 6.249257900624301E-5 44-45 4.999406320499441E-4 0.0 0.0 0.05508720839400322 6.249257900624301E-5 46-47 4.999406320499441E-4 0.0 0.0 0.07317881001631056 6.249257900624301E-5 48-49 4.999406320499441E-4 0.0 0.0 0.09305145014029584 6.249257900624301E-5 50-51 4.999406320499441E-4 0.0 0.0 0.11504883795049338 6.249257900624301E-5 52-53 4.999406320499441E-4 0.0 0.0 0.138608540235847 6.249257900624301E-5 54-55 4.999406320499441E-4 0.0 0.0 0.16788631350027183 1.2498515801248603E-4 56-57 4.999406320499441E-4 0.0 0.0 0.2041632556133959 1.2498515801248603E-4 58-59 4.999406320499441E-4 0.0 0.0 0.24828301639180347 1.2498515801248603E-4 60-61 5.62433211056187E-4 0.0 0.0 0.2974646760697167 1.5623144751560753E-4 62-63 5.62433211056187E-4 0.0 0.0 0.348208650222786 2.4997031602497205E-4 64-65 5.62433211056187E-4 0.0 0.0 0.3979527431117555 2.4997031602497205E-4 66-67 6.249257900624301E-4 0.0 0.0 0.46453858604290743 2.4997031602497205E-4 68-69 6.249257900624301E-4 0.0 0.0 0.5438729150913328 2.4997031602497205E-4 70-71 6.87418369068673E-4 0.0 0.0 0.6284253744867797 2.4997031602497205E-4 72-73 6.87418369068673E-4 0.0 0.0 0.7208206525475099 2.4997031602497205E-4 74-75 6.87418369068673E-4 0.0 0.0 0.8182465832182428 2.4997031602497205E-4 76-77 6.87418369068673E-4 0.0 0.0 0.9300458070604116 2.4997031602497205E-4 78-79 6.87418369068673E-4 0.0 0.0 1.0605928046044533 2.4997031602497205E-4 80-81 8.748961060874021E-4 0.0 0.0 1.2128247270636612 2.4997031602497205E-4 82-83 8.748961060874021E-4 0.0 0.0 1.3645879551803224 2.4997031602497205E-4 84-85 8.748961060874021E-4 0.0 0.0 1.5208818952749361 2.4997031602497205E-4 86-87 8.748961060874021E-4 0.0 0.0 1.6891744105387485 2.812166055280935E-4 88-89 8.748961060874021E-4 0.0 0.0 1.8768083790049932 3.1246289503121505E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 70 0.008389872 27.144625 1 TAAGGTG 820 0.0 25.483093 5 GGTAAGG 750 0.0 24.695433 3 GTAAGGT 775 0.0 23.898808 4 AGGTAAG 745 0.0 23.587717 2 GAGTACT 7680 0.0 20.778059 12-13 GTACATG 7190 0.0 20.018688 1 GTACTTT 7850 0.0 19.934835 14-15 AGTACTT 7855 0.0 19.71053 12-13 TACATGG 7190 0.0 19.684656 2 TAGACCG 150 7.732471E-4 18.996489 5 ACATGGG 7360 0.0 18.325417 3 AAGGTGT 1205 0.0 18.129429 6 AGAGTAC 12715 0.0 17.648409 10-11 TACTTTT 8775 0.0 17.644014 14-15 ACTTTTT 9110 0.0 17.30799 16-17 GTGTAAG 695 0.0 17.087442 1 CATGGGG 3725 0.0 16.956596 4 GGTGTGC 1535 0.0 16.707008 8 GTACCTG 1350 0.0 16.538115 1 >>END_MODULE