FastQCFastQC Report
Thu 26 May 2016
SRR936375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733165
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA359911.3168249995883892No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA300951.1011043972830032No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT192500.704311667974674No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG141330.5170928209603153No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA126290.46206504181050173No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT122240.4472470560686969No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC114560.41914776458794106No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG81600.29855497198303066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68270.2497836757019792No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG60870.2227088375564593No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58630.21451321087457217No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT56100.2052565432383336No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG54750.20031721465773197No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA53220.19471930893305014No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG52970.19380461845516095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47170.1725837993681318No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT46920.17166910889024262No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA42000.15366800028538344No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC35420.12959334690734003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32530.11901952498294102No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA30260.1107141354437072No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28390.10387225066909608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCT37950.028.1848954
GTATAGC23950.027.8684521
GTGTAGC24300.027.8566571
TCGAGCG350.00833593527.18169478-79
GTAGCCC22000.026.4607543
TACATGG130950.025.6609422
GTACATG132050.025.595231
ACATGGG136450.024.1756973
ATAGCCC22650.023.8208033
TAGCCCG1000.001689486323.6640874
TAGGACG9750.023.3000264
TGACGCG753.0040637E-522.19695594-95
AGGACGT10250.022.163445
GACGTGG6700.021.1909457
TGTAGCC28950.021.089242
GGACGTG10400.020.9328486
CCCTGGC47250.020.833657
TATAGCC28450.020.7944552
GTCTAGG8450.020.7273731
TACGTAG1656.5056345E-520.078625
Produced by FastQC (version 0.11.5)