##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936375_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2733165 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.864104801576193 33.0 31.0 34.0 30.0 34.0 2 31.94972531844949 33.0 31.0 34.0 30.0 34.0 3 32.034931297598206 34.0 31.0 34.0 30.0 34.0 4 35.506740354131566 37.0 35.0 37.0 33.0 37.0 5 35.52395482892544 37.0 35.0 37.0 33.0 37.0 6 35.62377170789177 37.0 35.0 37.0 33.0 37.0 7 35.58423512667549 37.0 35.0 37.0 33.0 37.0 8 35.59924629504622 37.0 35.0 37.0 33.0 37.0 9 37.27622005989393 39.0 37.0 39.0 34.0 39.0 10-11 37.208828409554485 39.0 37.0 39.0 34.0 39.0 12-13 37.142863676360555 39.0 37.0 39.0 34.0 39.0 14-15 38.33750651716966 40.0 38.0 41.0 33.5 41.0 16-17 38.29603060920215 40.0 38.0 41.0 33.5 41.0 18-19 38.33065200966645 40.0 38.0 41.0 33.5 41.0 20-21 38.26000570766858 40.0 38.0 41.0 34.0 41.0 22-23 38.112747309437964 40.0 38.0 41.0 33.0 41.0 24-25 38.29977077856624 40.0 38.0 41.0 34.0 41.0 26-27 38.21328203749133 40.0 38.0 41.0 33.5 41.0 28-29 38.07916554617083 40.0 38.0 41.0 33.5 41.0 30-31 37.99261680140057 40.0 38.0 41.0 33.0 41.0 32-33 37.84036858367497 40.0 38.0 41.0 33.0 41.0 34-35 37.59615848293096 40.0 38.0 41.0 32.5 41.0 36-37 37.38983852054303 40.0 37.0 41.0 32.0 41.0 38-39 37.19350149003079 40.0 37.0 41.0 31.5 41.0 40-41 37.01343113203923 40.0 37.0 41.0 31.0 41.0 42-43 36.900302945486274 40.0 37.0 41.0 31.0 41.0 44-45 36.74416985436298 40.0 37.0 41.0 30.0 41.0 46-47 36.47035725980685 40.0 36.5 41.0 30.0 41.0 48-49 36.30094615583033 40.0 36.5 41.0 30.0 41.0 50-51 35.99914202033174 39.5 36.5 40.5 29.5 41.0 52-53 35.9759648612506 39.5 36.0 40.5 28.5 41.0 54-55 36.103775293478435 40.0 36.0 41.0 28.0 41.0 56-57 36.06013211789263 40.0 36.0 41.0 28.0 41.0 58-59 35.899102322765 40.0 36.0 41.0 28.0 41.0 60-61 35.62728905865544 39.0 35.5 41.0 28.0 41.0 62-63 35.33149279315373 39.0 35.0 41.0 27.5 41.0 64-65 34.968185418736155 38.5 35.0 40.0 26.0 41.0 66-67 34.621063492324836 38.0 34.0 40.0 26.0 41.0 68-69 34.27865917352227 37.0 34.0 40.0 26.0 41.0 70-71 33.82452157114554 37.0 34.0 39.0 25.5 41.0 72-73 33.34116216913358 36.0 33.5 39.0 24.0 40.5 74-75 32.89291974688685 36.0 33.0 38.5 22.5 40.0 76-77 32.39036154055829 35.0 32.5 37.0 21.0 39.5 78-79 31.943186745037348 35.0 32.0 37.0 20.0 39.0 80-81 31.55400735045268 35.0 32.0 36.5 19.5 39.0 82-83 31.182024868604714 35.0 32.0 36.0 18.0 37.5 84-85 30.813143919229173 35.0 31.5 35.5 14.0 37.0 86-87 30.44005612540772 34.5 31.0 35.0 7.5 37.0 88-89 30.02404007807798 34.0 31.0 35.0 4.5 36.0 90-91 29.60804744682447 34.0 30.0 35.0 2.0 36.0 92-93 29.36038676772167 34.0 30.0 35.0 2.0 36.0 94-95 29.017547422127826 34.0 30.0 35.0 2.0 35.0 96-97 28.547261142302055 34.0 29.0 35.0 2.0 35.0 98-99 28.032305221236186 34.0 29.0 35.0 2.0 35.0 100-101 24.75672965225297 30.0 22.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4533199133091621 1101 2 0.37112240700625065 1101 3 0.4284768051648271 1101 4 0.33400189441407946 1101 5 0.46646812466522647 1101 6 0.3613398341513374 1101 7 0.38232395294487986 1101 8 0.3322366532778176 1101 9 0.4512905331421777 1101 10-11 0.6093092043909039 1101 12-13 0.4864845889286613 1101 14-15 0.6882632335721013 1101 16-17 0.717425212359089 1101 18-19 0.6984325417155972 1101 20-21 0.7912413147300867 1101 22-23 0.7380893258706251 1101 24-25 0.7168702916364253 1101 26-27 0.6564044469984296 1101 28-29 0.5787865796793241 1101 30-31 0.6640902900966168 1101 32-33 0.800302355968391 1101 34-35 0.5577766318918691 1101 36-37 0.5972858767130802 1101 38-39 0.6579355160517792 1101 40-41 0.574295440796746 1101 42-43 0.5322044679039735 1101 44-45 0.4444201144202111 1101 46-47 0.492101791965851 1101 48-49 0.5317590924524467 1101 50-51 0.6440087582466916 1101 52-53 0.6779901177967318 1101 54-55 0.6007858745056822 1101 56-57 0.5811071701169581 1101 58-59 0.6009797720181851 1101 60-61 0.5191837325502817 1101 62-63 0.562857256920104 1101 64-65 0.5010937617212718 1101 66-67 0.6308373864944983 1101 68-69 0.6324580933641428 1101 70-71 0.7676194070404989 1101 72-73 0.7952520361582813 1101 74-75 0.6422562642537812 1101 76-77 0.6739100911128446 1101 78-79 0.6339933120151287 1101 80-81 0.7017839857599775 1101 82-83 0.8583514059168778 1101 84-85 0.8066274473149697 1101 86-87 0.7544256146349007 1101 88-89 0.6157811759553233 1101 90-91 0.6018974489938209 1101 92-93 0.6848506575586626 1101 94-95 0.9542343726075195 1101 96-97 1.158793120902434 1101 98-99 1.2285029443060935 1101 100-101 1.452587433871681 1102 1 0.4890030760474424 1102 2 0.38563644588314006 1102 3 0.4135176540708301 1102 4 0.2907781175336197 1102 5 0.4973949722565507 1102 6 0.34606046519650846 1102 7 0.3710196051187964 1102 8 0.3937475932721881 1102 9 0.5170929559048787 1102 10-11 0.5362657261300328 1102 12-13 0.5595684465678801 1102 14-15 0.6763743238153168 1102 16-17 0.6432102625985507 1102 18-19 0.6288889225964382 1102 20-21 0.6438213691952939 1102 22-23 0.678764976166427 1102 24-25 0.782393345444298 1102 26-27 0.6699667533182705 1102 28-29 0.6610708317217728 1102 30-31 0.6941419381386922 1102 32-33 0.5565287622037189 1102 34-35 0.5808435865355008 1102 36-37 0.5748161612468436 1102 38-39 0.718005945200062 1102 40-41 0.6243592965578486 1102 42-43 0.6120462370841793 1102 44-45 0.6895628508273859 1102 46-47 0.7230164318381327 1102 48-49 0.5881409123416432 1102 50-51 0.5107845118385796 1102 52-53 0.621569796639811 1102 54-55 0.653337287783927 1102 56-57 0.6338243266574821 1102 58-59 0.7399017365218938 1102 60-61 0.6809172286532075 1102 62-63 0.7782821794480341 1102 64-65 0.6145433814038697 1102 66-67 0.7687451712630278 1102 68-69 0.8600686671269386 1102 70-71 0.8324677496078721 1102 72-73 0.8309305036200172 1102 74-75 0.9124074521587531 1102 76-77 0.8518089548559189 1102 78-79 0.9496468945111332 1102 80-81 0.9294236702373873 1102 82-83 0.9385988469911553 1102 84-85 1.0654357861261268 1102 86-87 1.1481325354725378 1102 88-89 1.0506285794673893 1102 90-91 1.0819697562527182 1102 92-93 1.0450642926417686 1102 94-95 1.1034404013426133 1102 96-97 1.149184706966853 1102 98-99 1.2627779936064947 1102 100-101 1.3501012849375122 1103 1 0.3175662230528964 1103 2 0.19707108180641697 1103 3 0.25465704697492697 1103 4 0.1501327498950502 1103 5 0.10379431222352764 1103 6 0.1367255047993936 1103 7 0.30623205779023266 1103 8 0.23801796329593117 1103 9 0.23635885089747433 1103 10-11 0.32313993732061164 1103 12-13 0.34200451722702496 1103 14-15 0.33074654282198424 1103 16-17 0.475013604742621 1103 18-19 0.3226788514593366 1103 20-21 0.3841639793969094 1103 22-23 0.49891215698767155 1103 24-25 0.5234559586262364 1103 26-27 0.47744073680473065 1103 28-29 0.4210128971053848 1103 30-31 0.4831439017005792 1103 32-33 0.531213533740889 1103 34-35 0.3415460891909845 1103 36-37 0.3761152378003061 1103 38-39 0.4547201002005963 1103 40-41 0.4925294222494401 1103 42-43 0.4443400608142909 1103 44-45 0.46587344433918076 1103 46-47 0.4421281313413914 1103 48-49 0.5463809212099306 1103 50-51 0.5005397344498022 1103 52-53 0.5978273258229265 1103 54-55 0.67449369034955 1103 56-57 0.6752341295652897 1103 58-59 0.6574945604800817 1103 60-61 0.6500575047512669 1103 62-63 0.6575443853794312 1103 64-65 0.6862280193715762 1103 66-67 0.6893495776012983 1103 68-69 0.7157906645627676 1103 70-71 0.647819696041978 1103 72-73 0.6414970289332516 1103 74-75 0.6147930753602182 1103 76-77 0.610521000004205 1103 78-79 0.6841918633494686 1103 80-81 0.7506666657914316 1103 82-83 0.8432345249379267 1103 84-85 0.7648767934033884 1103 86-87 0.8024056223172131 1103 88-89 0.7227850719879534 1103 90-91 0.8699465060807583 1103 92-93 0.9983237054268201 1103 94-95 0.8027190354086393 1103 96-97 1.0738471714959843 1103 98-99 1.1693583338178968 1103 100-101 1.292282610473535 1104 1 0.30016335176471287 1104 2 0.23584150812984817 1104 3 0.29472913468046613 1104 4 0.3270524290718484 1104 5 0.34837552302597175 1104 6 0.25748939357886513 1104 7 0.30110699588034606 1104 8 0.2430696699779631 1104 9 0.2835351910878572 1104 10-11 0.36880838114459635 1104 12-13 0.3683816740939392 1104 14-15 0.5122512738840328 1104 16-17 0.5657485943634626 1104 18-19 0.31567964226021417 1104 20-21 0.49673309131370047 1104 22-23 0.5876497485307013 1104 24-25 0.5911841927383108 1104 26-27 0.5156044506170971 1104 28-29 0.4747019210099097 1104 30-31 0.45183242402341506 1104 32-33 0.49405753518278317 1104 34-35 0.4419963572351904 1104 36-37 0.4160598729858549 1104 38-39 0.3105778652891473 1104 40-41 0.4798850118040008 1104 42-43 0.5119622887438666 1104 44-45 0.5156105647630795 1104 46-47 0.4322166845819595 1104 48-49 0.38944984133519256 1104 50-51 0.37686614665611273 1104 52-53 0.43982550472249216 1104 54-55 0.5827562376387192 1104 56-57 0.6024316920008346 1104 58-59 0.5212529812313065 1104 60-61 0.5794706926906841 1104 62-63 0.5364040914928196 1104 64-65 0.5672886449288512 1104 66-67 0.6152297221618781 1104 68-69 0.5621044593197411 1104 70-71 0.5321848958316835 1104 72-73 0.6271802418432131 1104 74-75 0.6949320861430834 1104 76-77 0.6796139214698229 1104 78-79 0.6897713250061344 1104 80-81 0.6608502797041389 1104 82-83 0.8082917884097753 1104 84-85 0.7108875207738699 1104 86-87 0.7455324738145457 1104 88-89 0.7502849665069888 1104 90-91 0.8939307768991576 1104 92-93 0.8145257919016053 1104 94-95 0.5855683030205903 1104 96-97 0.8423115489543243 1104 98-99 0.8405493898723222 1104 100-101 1.0489167504867822 1105 1 0.2704159600993812 1105 2 0.2331645463903662 1105 3 0.24311988880938884 1105 4 0.13574172952083785 1105 5 0.10766395300713327 1105 6 0.21702724166313914 1105 7 0.24622219831166348 1105 8 0.23193505367243716 1105 9 0.2172245758453002 1105 10-11 0.250042063245111 1105 12-13 0.240341258648904 1105 14-15 0.2103233420915842 1105 16-17 0.30589959753279317 1105 18-19 0.3038916355074335 1105 20-21 0.18167715434674392 1105 22-23 0.38984713011274863 1105 24-25 0.29680616648668234 1105 26-27 0.33044961647204474 1105 28-29 0.41090411896479395 1105 30-31 0.436407603887055 1105 32-33 0.46141996572105626 1105 34-35 0.11943789607338573 1105 36-37 0.32706919008070656 1105 38-39 0.3918926015284967 1105 40-41 0.34915591574565497 1105 42-43 0.3849748857744615 1105 44-45 0.3987397536281563 1105 46-47 0.38859886757715145 1105 48-49 0.5702387626659657 1105 50-51 0.44131902531736955 1105 52-53 0.49323886700397424 1105 54-55 0.5308316781840645 1105 56-57 0.6533318195426503 1105 58-59 0.6481307832224772 1105 60-61 0.559226717540767 1105 62-63 0.43556284496886377 1105 64-65 0.5617994907044235 1105 66-67 0.6167664964938027 1105 68-69 0.6517396802854307 1105 70-71 0.665629061046559 1105 72-73 0.7445515922149397 1105 74-75 0.7484069935892776 1105 76-77 0.7985704392208035 1105 78-79 0.7237688739910269 1105 80-81 0.7286242366231939 1105 82-83 0.6902216053390475 1105 84-85 0.6953562190717157 1105 86-87 0.6777777392209039 1105 88-89 0.6593887441323361 1105 90-91 0.673013649402531 1105 92-93 0.6055634332615725 1105 94-95 0.4040779354290258 1105 96-97 0.40606744970237685 1105 98-99 0.44591926450903685 1105 100-101 0.6535757363168919 1106 1 0.11917169353501222 1106 2 0.03256799874572991 1106 3 0.07585740398376828 1106 4 0.09101159845801021 1106 5 0.11306203978074336 1106 6 -0.032783521168617824 1106 7 0.05529283784318295 1106 8 1.4044165369142547E-4 1106 9 0.07417929924363875 1106 10-11 0.06529670309588198 1106 12-13 0.08324737512889158 1106 14-15 0.07999010444960675 1106 16-17 0.11575566786207503 1106 18-19 0.10204444902644383 1106 20-21 0.029792442264017893 1106 22-23 0.04921668713042493 1106 24-25 0.06631481282301621 1106 26-27 0.01920532416112053 1106 28-29 0.02634796380061033 1106 30-31 0.005291381369886494 1106 32-33 0.056465459881088975 1106 34-35 -0.010658634192331817 1106 36-37 0.10094509555481324 1106 38-39 0.10152152288950589 1106 40-41 0.00923656038786902 1106 42-43 -0.022965093860015884 1106 44-45 0.03852084999365246 1106 46-47 0.15530516248075088 1106 48-49 0.07956722174760955 1106 50-51 -0.041304464808177954 1106 52-53 -0.0060883433698322165 1106 54-55 0.00641525600238424 1106 56-57 0.07047088182742556 1106 58-59 -0.05759190322441299 1106 60-61 0.11484281325615342 1106 62-63 -0.035223345352235924 1106 64-65 -0.013226609864183558 1106 66-67 0.048746354964990246 1106 68-69 0.18202728216132869 1106 70-71 0.19661101528720337 1106 72-73 0.2227832030609349 1106 74-75 0.11921416735147972 1106 76-77 0.19010600229149333 1106 78-79 0.1756373013786643 1106 80-81 0.17889332029048077 1106 82-83 0.21583025386383525 1106 84-85 0.2008637286977084 1106 86-87 0.23282306602834169 1106 88-89 0.11691803405526358 1106 90-91 0.2855376741552682 1106 92-93 0.2544036153926932 1106 94-95 0.24629967633373795 1106 96-97 0.3852803260687132 1106 98-99 0.5661012797690184 1106 100-101 0.7843405505619465 1107 1 -0.01268198897907169 1107 2 0.10451744276560859 1107 3 0.18902810292780714 1107 4 0.16275370067334194 1107 5 0.10884269253485002 1107 6 0.21203051110987303 1107 7 0.11186941580453436 1107 8 0.1639750520632859 1107 9 0.1867164445028635 1107 10-11 0.14897081658018152 1107 12-13 0.15247990637301 1107 14-15 0.30277010193009346 1107 16-17 0.10500939775902651 1107 18-19 0.19962458137099048 1107 20-21 0.3620773155349042 1107 22-23 0.3546126197558621 1107 24-25 0.34655229855051317 1107 26-27 0.310844186531277 1107 28-29 0.35328046126188895 1107 30-31 0.2925675449483691 1107 32-33 0.3909106309222139 1107 34-35 0.29316577787420073 1107 36-37 0.17186618437161627 1107 38-39 0.08892287680977518 1107 40-41 0.17553320853466658 1107 42-43 -0.07269822273639903 1107 44-45 0.11921799629542562 1107 46-47 0.13144239301576022 1107 48-49 0.03249787535381188 1107 50-51 0.060278901467050616 1107 52-53 0.015160098225102558 1107 54-55 0.13074106098480343 1107 56-57 0.14073686410463182 1107 58-59 0.1690637653601783 1107 60-61 0.2518665076961568 1107 62-63 0.32603407826962183 1107 64-65 0.08357761970813726 1107 66-67 0.15085567772751318 1107 68-69 0.13480623040206297 1107 70-71 0.234693907232554 1107 72-73 0.18987734025002823 1107 74-75 0.21260544389198088 1107 76-77 0.24135818973916656 1107 78-79 0.23530671400155256 1107 80-81 0.26686355262357253 1107 82-83 0.40360473949409226 1107 84-85 0.4106487748926604 1107 86-87 0.22331433077325258 1107 88-89 0.36350867316591007 1107 90-91 0.49447322042657404 1107 92-93 0.254318657046511 1107 94-95 0.35109934672542664 1107 96-97 0.4362788080126023 1107 98-99 0.397376844545704 1107 100-101 0.2765064220006721 1108 1 0.008352137603331755 1108 2 0.038586665847486046 1108 3 0.09397227413462872 1108 4 0.04402021482498242 1108 5 0.09790136966800844 1108 6 0.026549701001208348 1108 7 8.164448479135444E-4 1108 8 0.05477043153541672 1108 9 0.14600018734441988 1108 10-11 0.04514458241295216 1108 12-13 0.07392398435510472 1108 14-15 0.06115834921969565 1108 16-17 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2316 70-71 -1.7552271246185427 2316 72-73 -1.8387718200271443 2316 74-75 -1.7519173584507257 2316 76-77 -2.0753457943000413 2316 78-79 -2.0764309458058783 2316 80-81 -1.8916176982060584 2316 82-83 -2.0195369829437837 2316 84-85 -2.0662839267142097 2316 86-87 -2.0949364864514557 2316 88-89 -1.9484234162393967 2316 90-91 -2.199235840788667 2316 92-93 -2.3397075433075116 2316 94-95 -2.2943730891040275 2316 96-97 -2.643471039171118 2316 98-99 -2.6904790546777235 2316 100-101 -2.631442108118595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15066.0 3 3337.0 4 2814.0 5 2317.0 6 2262.0 7 2439.0 8 2951.0 9 3678.0 10 4734.0 11 6262.0 12 8980.0 13 14021.0 14 14415.0 15 13091.0 16 16169.0 17 20830.0 18 19343.0 19 13592.0 20 12172.0 21 11929.0 22 12724.0 23 13865.0 24 15617.0 25 17971.0 26 22087.0 27 25996.0 28 31703.0 29 38484.0 30 46429.0 31 58753.0 32 74141.0 33 97168.0 34 132155.0 35 192616.0 36 311370.0 37 570960.0 38 721703.0 39 158864.0 40 157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.842818618649616 18.06415621866836 12.983593979639535 23.10943118304249 2 14.292807983583186 21.011977423953677 41.39065020301463 23.304564389448508 3 21.07061924345385 25.582181642941276 28.916774947335526 24.430424166269344 4 11.695820200643835 15.43482222113266 39.794994661548 33.07436291667551 5 12.530123391559084 39.37728280970652 35.08393567919512 13.008658119539277 6 30.706957537029915 36.247366897756415 18.785997622386887 14.259677942826784 7 27.58799853640688 33.34427369191365 21.169703622392973 17.8980241492865 8 23.860580983312868 34.82368808921271 21.144366171414518 20.171364756059898 9 25.87559523569925 17.617732525694898 21.177528349741156 35.32914388886469 10-11 23.71668101551029 25.816685808621674 28.869220870597335 21.5974123052707 12-13 27.259564482627585 23.28751709944398 27.652131308361543 21.800787109566887 14-15 23.401194515365045 25.049274444890653 27.952135225945263 23.59739581379904 16-17 20.94126464276154 28.109820551437508 29.252349804025187 21.696565001775767 18-19 20.130059506166877 29.08646580499454 30.159036835861485 20.624437852977103 20-21 22.30827548002936 27.631206317310035 28.5494255161427 21.511092686517905 22-23 20.913040956632358 27.974533079315457 27.54046694721335 23.57195901683884 24-25 21.27356258574825 27.369835571952045 29.355773316113847 22.000828526185856 26-27 22.56572211818309 26.532595228738508 29.467413552993726 21.43426910008467 28-29 20.84474318546848 27.246269467802904 30.30525646688401 21.603730879844605 30-31 21.214308493616574 28.69300981926385 29.546420525476165 20.54626116164341 32-33 20.639239698054272 28.31844450160988 28.953468756872507 22.08884704346334 34-35 21.621732177506424 27.302613597634146 28.867521735956238 22.20813248890319 36-37 22.230161249674406 27.251178430877676 29.26677638333162 21.251883936116297 38-39 22.768487531970134 26.358094155508756 30.04153865440347 20.831879658117646 40-41 22.082375150667865 26.807701839784258 30.467359034078335 20.642563975469542 42-43 20.635838054652776 28.233811982196773 29.669115153132893 21.46123481001756 44-45 21.44056841250209 28.847202919284477 27.99472757837248 21.717501089840955 46-47 21.132626120040914 27.956842741851702 27.048453727101574 23.862077411005806 48-49 21.878418537374905 27.313894112755715 28.25830916624511 22.549378183624267 50-51 23.07847918014523 28.211288487702213 27.73529207307721 20.974940259075343 52-53 21.557747621546696 28.23854519456629 29.091640282314106 21.11206690157291 54-55 20.70663120609755 27.687006851562952 29.626331035383043 21.980030906956454 56-57 21.85495250606027 29.04836540503372 27.43872854315454 21.657953545751468 58-59 21.491466429770128 28.354191700874974 27.858477606364435 22.295864262990456 60-61 21.564735018282306 27.22218371725037 28.633935316182097 22.57914594828523 62-63 23.567141975767104 26.765146662921257 28.087407262515196 21.580304098796443 64-65 22.66727735440071 27.36694971957335 28.617695075475158 21.348077850550787 66-67 21.12176143658992 27.580386775622895 29.35336726037457 21.944484527412612 68-69 21.355895990594746 28.94761616000406 28.464280368958395 21.2322074804428 70-71 21.46356448235374 28.289039131370746 27.404478706446422 22.842917679829092 72-73 21.302050269182978 27.564215348586572 27.35424104520542 23.77949333702503 74-75 21.993892786725425 27.92578156387981 27.409148904833273 22.671176744561492 76-77 22.70367125056672 26.672944571444752 28.277413772879818 22.34597040510871 78-79 21.698428354879066 26.645125528454987 29.079378328266724 22.577067788399223 80-81 21.348179918133457 28.67467314421367 28.08198274124965 21.895164196403226 82-83 21.53827117775898 28.335723755563226 27.766673953455623 22.359331113222176 84-85 22.02497010974133 27.0522146921262 27.69214109121369 23.230674106918784 86-87 22.30829749127996 27.376777217636842 28.237685168109998 22.07724012297319 88-89 23.59142485067741 26.064492354675956 28.46722442208176 21.87685837256488 90-91 22.076786278871 26.99772870525532 29.12870676350707 21.796778252366614 92-93 21.40338442684031 28.17751615966715 28.761982281928173 21.65711713156437 94-95 21.4083343843639 28.86170999425273 27.431235942083127 22.298719679300238 96-97 21.190973999656283 27.80504750937643 27.093679722746327 23.910298768220965 98-99 22.18907272128119 27.273315399153137 27.38934171560522 23.148270163960458 100-101 23.028728103590787 27.691023039719614 27.281469686673855 21.99877917001574 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 313.0 1 202.0 2 174.5 3 256.5 4 185.5 5 161.5 6 315.0 7 555.0 8 769.5 9 846.0 10 874.0 11 1016.5 12 1243.0 13 1503.0 14 1747.5 15 2012.0 16 2659.5 17 2911.0 18 2720.5 19 2871.5 20 3068.0 21 3366.0 22 3964.5 23 5051.5 24 6540.5 25 8865.5 26 12084.5 27 15436.0 28 19318.0 29 23942.5 30 29812.0 31 37034.5 32 44245.5 33 51763.0 34 59935.0 35 68337.0 36 75813.5 37 81827.0 38 85780.0 39 87781.0 40 89978.0 41 92622.5 42 94396.5 43 96514.0 44 100974.5 45 104041.0 46 108842.5 47 125002.0 48 215913.5 49 214011.0 50 121147.0 51 101178.5 52 87946.5 53 77764.5 54 68460.5 55 60054.5 56 51841.0 57 43662.0 58 35066.0 59 27007.0 60 20400.0 61 15067.5 62 10645.0 63 7088.0 64 4475.5 65 2772.0 66 1851.0 67 1585.0 68 1242.5 69 840.0 70 726.5 71 615.0 72 450.0 73 344.0 74 241.0 75 178.5 76 155.0 77 113.0 78 108.5 79 118.5 80 85.5 81 70.5 82 55.0 83 43.5 84 35.5 85 29.5 86 26.5 87 20.5 88 18.5 89 16.5 90 16.5 91 16.0 92 16.0 93 18.5 94 22.5 95 21.0 96 19.0 97 17.5 98 12.0 99 9.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04763708008846886 2 0.013061780024257593 3 0.01090311049643911 4 0.0059637819158375 5 0.008744440968620629 6 0.006293070487877607 7 0.0060369571540686345 8 0.010281120971474463 9 0.015513150505000614 10-11 0.0027806590527831286 12-13 0.009311549064911924 14-15 0.009421311922258627 16-17 0.019885370989310928 18-19 0.012403202880177376 20-21 0.0037685247689034506 22-23 0.010482352876610083 24-25 0.022025746707571625 26-27 0.00801268858630928 28-29 0.018732860987170554 30-31 0.015531444314558395 32-33 0.013025192405142024 34-35 0.2700898043111191 36-37 0.4803405575587277 38-39 0.4773952542199245 40-41 0.4978477333055268 42-43 0.48740196804803226 44-45 0.5024028918854149 46-47 0.5085861995159457 48-49 0.5149524452420545 50-51 0.5247396333554689 52-53 0.5146414504795722 54-55 0.5017077271222191 56-57 0.4978660271150845 58-59 0.4958720018732861 60-61 0.492414471866865 62-63 0.48994480757656417 64-65 0.5058787157013938 66-67 0.47975515565287863 68-69 0.5061531228447606 70-71 0.48806054519211245 72-73 0.49563418234903495 74-75 0.5093545395173726 76-77 0.496640341874713 78-79 0.496219584254884 80-81 0.4982136094966824 82-83 0.49874412997385814 84-85 0.4988173052120893 86-87 0.501634551883988 88-89 0.5141292238119543 90-91 0.5367952538540484 92-93 0.5253616228804335 94-95 0.4980306714011046 96-97 0.4724376318297651 98-99 0.46325413943175775 100-101 0.47141317849452924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2733165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.45071820739654 #Duplication Level Percentage of deduplicated Percentage of total 1 61.55773767098849 22.4382374933004 2 15.087826080061639 10.999241936130705 3 7.374924925123773 8.06463930739175 4 4.322665967398274 6.302571163293508 5 2.8724350694118885 5.235116064208814 6 1.9008088665524114 4.157150901649366 7 1.3665414242953795 3.486799146300764 8 1.0232184559872226 2.9837638083038107 9 0.8145663635074619 2.672237607969081 >10 3.594348143565574 21.093632881576163 >50 0.05783331282306363 1.3913673030035156 >100 0.019952605010936573 1.4363651530601464 >500 0.002514477209120709 0.6152583288655147 >1k 0.0031179517393009723 2.3624863968542376 >5k 8.046327069163799E-4 1.7832666174641056 >10k+ 7.040536185518324E-4 4.977865890628099 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 35991 1.3168249995883892 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 30095 1.1011043972830032 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 19250 0.704311667974674 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 14133 0.5170928209603153 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 12629 0.46206504181050173 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 12224 0.4472470560686969 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 11456 0.41914776458794106 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 8160 0.29855497198303066 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6827 0.2497836757019792 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6087 0.2227088375564593 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5863 0.21451321087457217 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5610 0.2052565432383336 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5475 0.20031721465773197 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5322 0.19471930893305014 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5297 0.19380461845516095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4717 0.1725837993681318 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4692 0.17166910889024262 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4200 0.15366800028538344 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3542 0.12959334690734003 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3253 0.11901952498294102 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3026 0.1107141354437072 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2839 0.10387225066909608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0976285734670245E-4 0.0 5 0.0 0.0 0.0 1.0976285734670245E-4 0.0 6 3.658761911556748E-5 0.0 0.0 1.0976285734670245E-4 0.0 7 1.4635047646226993E-4 0.0 0.0 7.683400014269171E-4 0.0 8 1.8293809557783742E-4 0.0 0.0 7.683400014269171E-4 0.0 9 2.9270095292453985E-4 0.0 0.0 0.0013903295263915643 0.0 10-11 4.3905142938680984E-4 0.0 0.0 0.0014635047646226994 0.0 12-13 5.488142867335123E-4 0.0 0.0 0.0016281490506427532 0.0 14-15 5.488142867335123E-4 0.0 0.0 0.0017196180984316717 0.0 16-17 6.768709536379984E-4 0.0 0.0 0.0022501385756074003 0.0 18-19 7.500461918691334E-4 0.0 0.0 0.00283554048145648 0.0 20-21 0.001134216192582592 0.0 0.0 0.003329473339516641 0.0 22-23 0.0016464428602005368 0.0 0.0 0.004902740961486043 0.0 24-25 0.0018293809557783742 0.0 0.0 0.006366245726108742 0.0 26-27 0.002048906670471779 0.0 0.0 0.007207760965766794 0.0 28-29 0.0023781952425118865 0.0 0.0 0.008963966683314034 0.0 30-31 0.0026160147667630747 0.0 0.0 0.014854573360920397 0.0 32-33 0.002762365243225345 0.0 0.0 0.025885740524263994 0.0 34-35 0.0027806590527831286 0.0 0.0 0.046649214372348535 0.0 36-37 0.0030733600057076685 0.0 0.0 0.07778527823969647 0.0 38-39 0.003109947624823236 0.0 0.0 0.11872682403001648 0.0 40-41 0.0033660609586322085 0.0 0.0 0.16221120934886846 0.0 42-43 0.0036587619115567484 0.0 0.0 0.20551265657214254 0.0 44-45 0.003841700007134586 0.0 0.0 0.25697314285818823 0.0 46-47 0.003988050483596856 0.0 0.0 0.31827569868632155 0.0 48-49 0.00420757619829026 0.0 0.0 0.39520116787680215 0.0 50-51 0.004573452389445935 0.0 0.0 0.46566892229338513 0.0 52-53 0.004738096675465989 0.0 0.0 0.5323132705123913 0.0 54-55 0.00479297810413934 0.0 0.0 0.6031834887392455 0.0 56-57 0.00479297810413934 0.0 0.0 0.6855056317492724 0.0 58-59 0.004811271913697124 0.0 0.0 0.7964027052885574 0.0 60-61 0.004829565723254907 0.0 0.0 0.9362588793578142 0.0 62-63 0.00537838000998842 0.0 0.0 1.0611873048279192 0.0 64-65 0.005634493343797392 0.0 0.0 1.1724319607488023 0.0 66-67 0.005707668582028528 0.0 0.0 1.303068054800936 0.0 68-69 0.005707668582028528 0.0 0.0 1.4506442165035773 0.0 70-71 0.005725962391586311 0.0 0.0 1.617227646336756 0.0 72-73 0.005744256201144095 0.0 0.0 1.796744799527288 0.0 74-75 0.005780843820259662 0.0 0.0 1.9805061165352256 0.0 76-77 0.005890606677606365 0.0 0.0 2.1635905625895253 0.0 78-79 0.0059637819158375 0.0 0.0 2.3809759015646694 0.0 80-81 0.0059637819158375 0.0 0.0 2.618466137243818 0.0 82-83 0.006000369534953067 3.658761911556748E-5 0.0 2.8597066038823122 0.0 84-85 0.00625648286876204 3.658761911556748E-5 0.0 3.1694939749338222 0.0 86-87 0.006329658106993175 3.658761911556748E-5 0.0 3.520844881300617 0.0 88-89 0.006366245726108742 3.658761911556748E-5 0.0 3.847096680954132 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCCCT 3795 0.0 28.184895 4 GTATAGC 2395 0.0 27.868452 1 GTGTAGC 2430 0.0 27.856657 1 TCGAGCG 35 0.008335935 27.181694 78-79 GTAGCCC 2200 0.0 26.460754 3 TACATGG 13095 0.0 25.660942 2 GTACATG 13205 0.0 25.59523 1 ACATGGG 13645 0.0 24.175697 3 ATAGCCC 2265 0.0 23.820803 3 TAGCCCG 100 0.0016894863 23.664087 4 TAGGACG 975 0.0 23.300026 4 TGACGCG 75 3.0040637E-5 22.196955 94-95 AGGACGT 1025 0.0 22.16344 5 GACGTGG 670 0.0 21.190945 7 TGTAGCC 2895 0.0 21.08924 2 GGACGTG 1040 0.0 20.932848 6 CCCTGGC 4725 0.0 20.83365 7 TATAGCC 2845 0.0 20.794455 2 GTCTAGG 845 0.0 20.727373 1 TACGTAG 165 6.5056345E-5 20.07862 5 >>END_MODULE