FastQCFastQC Report
Thu 26 May 2016
SRR936375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733165
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA361391.3222399672174934No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA308351.1281792354285234No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT193760.7089217079832355No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG147090.5381672895708821No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA127970.4682117618219171No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT123010.4500643027405956No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC120390.44047834653231693No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG80010.2927375405436554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67390.24656396521980928No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG66360.24279544045090581No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59890.21912325088313364No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT57780.21140326324974892No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG56450.20653710990737842No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG55150.2017807194223547No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA54560.1996220498945362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50360.18425524986599784No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT46690.17082759365058459No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA46550.17031536698296662No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC36050.13189836691162077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33750.12348321451504025No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA30950.11323868116268138No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28060.10266485923828236No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT27750.10153064304569977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCT40400.029.7076634
GTATAGC23850.028.5276491
ATAGCCC22700.028.0032373
GTGTAGC26050.026.8489881
GTAGCCC24400.026.2466133
GTACCGT1401.798641E-523.719166
TACGACT1601.882272E-623.719164
GTACATG131800.023.6091671
TACATGG130250.023.4966872
TATAGCC28300.023.1375482
ACATGGG133750.022.0965043
CCCTGGC49600.021.6150427
CCCTGCG3101.0913936E-1121.4237589
TATAACG1150.003747943320.6298872
GTCCTAC12750.020.5244161
AGCCCTG56950.020.241465
TGTAGCC31300.020.1619242
CATGGGG40600.019.8633374
TACGATA1200.00480109719.7659684
TACCGTC1200.00480109719.7659687
Produced by FastQC (version 0.11.5)