##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936375_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2733165 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47405114583276 34.0 31.0 34.0 31.0 34.0 2 32.597316298137876 34.0 31.0 34.0 31.0 34.0 3 32.70177834122711 34.0 31.0 34.0 31.0 34.0 4 36.14421595476307 37.0 35.0 37.0 35.0 37.0 5 36.130079962241574 37.0 35.0 37.0 35.0 37.0 6 36.18196413315698 37.0 36.0 37.0 35.0 37.0 7 36.14095307089034 37.0 36.0 37.0 35.0 37.0 8 36.13350309988603 37.0 36.0 37.0 35.0 37.0 9 37.898544361573485 39.0 38.0 39.0 35.0 39.0 10-11 37.84096953531895 39.0 38.0 39.0 35.0 39.0 12-13 37.80317617121542 39.0 38.0 39.0 35.0 39.0 14-15 39.09361985097863 40.0 38.0 41.0 36.0 41.0 16-17 39.03047492558993 40.0 38.0 41.0 35.5 41.0 18-19 39.1271957602267 40.0 38.5 41.0 36.0 41.0 20-21 39.09825129474437 40.0 39.0 41.0 36.0 41.0 22-23 38.99366393905966 40.0 38.5 41.0 35.0 41.0 24-25 39.15096399229465 40.5 39.0 41.0 36.0 41.0 26-27 39.01707580771743 40.5 38.5 41.0 35.5 41.0 28-29 38.91763047602322 40.0 38.0 41.0 35.0 41.0 30-31 38.8695693454292 40.0 38.0 41.0 35.0 41.0 32-33 38.74386544537194 40.0 38.0 41.0 35.0 41.0 34-35 38.62714783044565 40.0 38.0 41.0 35.0 41.0 36-37 38.4718985132621 40.0 38.0 41.0 34.0 41.0 38-39 38.301643332912576 40.0 38.0 41.0 34.0 41.0 40-41 38.159249807457655 40.0 38.0 41.0 33.5 41.0 42-43 38.23905545402491 40.0 38.0 41.0 34.0 41.0 44-45 38.14470805824017 40.0 38.0 41.0 33.5 41.0 46-47 37.94595459842344 40.0 38.0 41.0 33.0 41.0 48-49 37.74520784511729 40.0 38.0 41.0 33.0 41.0 50-51 37.58904859384633 40.0 38.0 41.0 33.0 41.0 52-53 37.323046541280895 40.0 37.0 41.0 32.0 41.0 54-55 37.18884059323166 40.0 37.0 41.0 31.5 41.0 56-57 37.065162915521014 40.0 37.0 41.0 31.5 41.0 58-59 36.885003649615 40.0 37.0 41.0 31.0 41.0 60-61 36.63757749715074 39.5 36.0 41.0 31.0 41.0 62-63 36.35775611790726 39.0 36.0 41.0 30.5 41.0 64-65 35.95481081456846 39.0 35.0 40.5 29.0 41.0 66-67 35.62730241313642 38.0 35.0 40.0 29.0 41.0 68-69 35.30525581148595 37.5 35.0 40.0 29.0 41.0 70-71 34.89341258211634 37.0 34.5 39.0 29.0 41.0 72-73 34.41480426538464 36.5 34.0 39.0 28.0 40.5 74-75 33.96340177047489 36.0 34.0 38.5 28.0 40.0 76-77 33.10383785830713 35.0 33.0 37.0 26.0 39.0 78-79 33.084626431261924 35.0 33.0 37.0 26.0 39.0 80-81 32.929816714322044 35.0 33.0 36.5 26.0 39.0 82-83 32.62729838849832 35.0 33.0 36.0 26.0 37.5 84-85 32.30219324482788 35.0 33.0 36.0 26.0 37.0 86-87 31.864278226890804 35.0 33.0 35.0 25.5 37.0 88-89 31.287571368724535 35.0 32.0 35.0 23.0 36.0 90-91 31.02569365552391 35.0 32.0 35.0 22.0 36.0 92-93 30.829084047249253 34.0 32.0 35.0 20.0 36.0 94-95 30.721370828325405 34.0 32.0 35.0 20.0 35.0 96-97 30.446607504486558 34.0 32.0 35.0 18.5 35.0 98-99 30.030916538152653 34.0 31.0 35.0 3.5 35.0 100-101 26.801950668913143 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14555953753522033 1101 2 0.1377965394032259 1101 3 0.22439780274797272 1101 4 0.21217063232545996 1101 5 0.1875675851880203 1101 6 0.20006332483913525 1101 7 0.17661070067198636 1101 8 0.15052114820732498 1101 9 0.25754452766773994 1101 10-11 0.162635792209322 1101 12-13 0.28099131892854956 1101 14-15 0.3683226122551133 1101 16-17 0.4469264396424393 1101 18-19 0.39769960354348655 1101 20-21 0.2694354666745582 1101 22-23 0.37656282610216607 1101 24-25 0.34038054133900175 1101 26-27 0.23805663532528598 1101 28-29 0.3176825207641514 1101 30-31 0.326091594855221 1101 32-33 0.34551689512347394 1101 34-35 0.41875755076886634 1101 36-37 0.3256607749008751 1101 38-39 0.331426426456801 1101 40-41 0.3834873598506974 1101 42-43 0.34950261841940033 1101 44-45 0.2514122265618184 1101 46-47 0.26240820971293743 1101 48-49 0.22182856817083518 1101 50-51 0.1986346339935423 1101 52-53 0.1861587090963681 1101 54-55 0.25928631510533506 1101 56-57 0.21251292321237258 1101 58-59 0.1288841881975955 1101 60-61 0.30232344393967736 1101 62-63 0.3004201207853896 1101 64-65 0.4034885926971228 1101 66-67 0.36503283033824374 1101 68-69 0.4480582711102983 1101 70-71 0.5404123380122385 1101 72-73 0.48232010703109296 1101 74-75 0.42377084538185983 1101 76-77 0.23274527256341315 1101 78-79 0.21928038926054683 1101 80-81 0.3816654597244806 1101 82-83 0.3653327325682767 1101 84-85 0.4195930429074721 1101 86-87 0.28875082155598975 1101 88-89 0.47567676362659483 1101 90-91 0.694336118704733 1101 92-93 0.7478469832133214 1101 94-95 0.6243214605918688 1101 96-97 0.5854378161885769 1101 98-99 0.651640023631213 1101 100-101 0.9202458381584364 1102 1 0.09213198565241498 1102 2 0.17174902320452645 1102 3 0.21173745905372954 1102 4 0.16094422609013037 1102 5 0.1551354319341982 1102 6 0.16854393334697448 1102 7 0.16665201910564065 1102 8 0.24272041574418068 1102 9 0.19812486384954298 1102 10-11 0.26545108002977 1102 12-13 0.3502203545187399 1102 14-15 0.4229169403610413 1102 16-17 0.3982246836090084 1102 18-19 0.3860318413122883 1102 20-21 0.32469605452318007 1102 22-23 0.36657268618292704 1102 24-25 0.31598764059715023 1102 26-27 0.40526106766635195 1102 28-29 0.4493554477995616 1102 30-31 0.38220531445330863 1102 32-33 0.391455480906167 1102 34-35 0.39003748315688114 1102 36-37 0.36945784504830925 1102 38-39 0.4038919161741461 1102 40-41 0.4263139163348697 1102 42-43 0.5151322550049926 1102 44-45 0.5629532163261146 1102 46-47 0.6233867053463271 1102 48-49 0.631874112353394 1102 50-51 0.5828204730394617 1102 52-53 0.6032110144771536 1102 54-55 0.5843262249560226 1102 56-57 0.7608892965807641 1102 58-59 0.5411482505508687 1102 60-61 0.4423070104717439 1102 62-63 0.5785678341857121 1102 64-65 0.6526439124454555 1102 66-67 0.5314123025891533 1102 68-69 0.46791600423077995 1102 70-71 0.4854494306969954 1102 72-73 0.44792438912330823 1102 74-75 0.5367204181116918 1102 76-77 0.430459611937998 1102 78-79 0.4385897140797752 1102 80-81 0.5529392882529791 1102 82-83 0.5639739195341917 1102 84-85 0.5433548984807643 1102 86-87 0.41513592813876343 1102 88-89 0.6649738807267909 1102 90-91 0.830327291584819 1102 92-93 0.7877591815418015 1102 94-95 0.6445407300068382 1102 96-97 0.6060097008725869 1102 98-99 0.6191487098928334 1102 100-101 0.8675052052351582 1103 1 -0.00703415930588136 1103 2 0.023147822533225337 1103 3 0.10851589094829706 1103 4 0.040655752407403156 1103 5 0.1143325977175138 1103 6 0.06180812555615489 1103 7 0.13203953862995377 1103 8 0.14193835294109647 1103 9 0.053033177957658495 1103 10-11 0.14497597256087857 1103 12-13 0.20888142520060882 1103 14-15 0.2828847201368063 1103 16-17 0.3031504889556018 1103 18-19 0.1750706046226682 1103 20-21 0.16671427993936305 1103 22-23 0.21713799392422573 1103 24-25 0.19338414471190646 1103 26-27 0.19316885203981116 1103 28-29 0.26322613620926916 1103 30-31 0.3463005721923835 1103 32-33 0.33350687152777425 1103 34-35 0.21508357370607456 1103 36-37 0.251731360769071 1103 38-39 0.25629911947503103 1103 40-41 0.28590070513430277 1103 42-43 0.2577430018036395 1103 44-45 0.26326860380020634 1103 46-47 0.34562856419702825 1103 48-49 0.3765381033906081 1103 50-51 0.39163832882261573 1103 52-53 0.3600097452947182 1103 54-55 0.38100806886875915 1103 56-57 0.35861670766848874 1103 58-59 0.33242171266211784 1103 60-61 0.3678758391766408 1103 62-63 0.343393635079984 1103 64-65 0.33834153118370836 1103 66-67 0.4124587965726292 1103 68-69 0.4256558091103386 1103 70-71 0.2437523171602365 1103 72-73 0.3127728150481417 1103 74-75 0.4092658976033263 1103 76-77 0.3775796454484919 1103 78-79 0.2795660068640302 1103 80-81 0.46612531961363857 1103 82-83 0.47794764723967376 1103 84-85 0.3790004069578856 1103 86-87 0.3560703328142445 1103 88-89 0.34861625622778547 1103 90-91 0.572771303720554 1103 92-93 0.7016556752072489 1103 94-95 0.5217604136016938 1103 96-97 0.38137222382699676 1103 98-99 0.35985095813030554 1103 100-101 0.8217664798148938 1104 1 0.009270659502327305 1104 2 0.036972569365772756 1104 3 4.056914378338661E-4 1104 4 0.03556693043108794 1104 5 0.041963901386978364 1104 6 0.04651522373363548 1104 7 0.05022429961533703 1104 8 0.1458174264102965 1104 9 0.02843309087677426 1104 10-11 0.08385347300687584 1104 12-13 0.18427381001757936 1104 14-15 0.2034105458164177 1104 16-17 0.17718888332696991 1104 18-19 0.26767825341953966 1104 20-21 0.17780308685946977 1104 22-23 0.15555855607738067 1104 24-25 0.18319188223534866 1104 26-27 0.2601695738622567 1104 28-29 0.23916264524623188 1104 30-31 0.2779444418399706 1104 32-33 0.18872194677991416 1104 34-35 0.28695961702662487 1104 36-37 0.24135483486215747 1104 38-39 0.2967484876484292 1104 40-41 0.3269685156523181 1104 42-43 0.3425546005431954 1104 44-45 0.3408529618745533 1104 46-47 0.4072970262285196 1104 48-49 0.3846752815182839 1104 50-51 0.4501589061895288 1104 52-53 0.4739111562186267 1104 54-55 0.30413695470438995 1104 56-57 0.4125158181444277 1104 58-59 0.4319998588267069 1104 60-61 0.4021675699415965 1104 62-63 0.2990865522388262 1104 64-65 0.22866102208791972 1104 66-67 0.298362092673365 1104 68-69 0.511062215455226 1104 70-71 0.6064927809368399 1104 72-73 0.42031464284682585 1104 74-75 0.350619530901767 1104 76-77 0.26467745877992144 1104 78-79 0.2931938850719433 1104 80-81 0.3805110150991098 1104 82-83 0.3145905443615078 1104 84-85 0.4427278924614697 1104 86-87 0.33430212518007707 1104 88-89 0.4378893059197395 1104 90-91 0.59957721226532 1104 92-93 0.6192816934487197 1104 94-95 0.576663912961191 1104 96-97 0.5242854522956186 1104 98-99 0.3349416936451455 1104 100-101 0.366750479096396 1105 1 -0.0025237546922483034 1105 2 0.06094078575655004 1105 3 0.0990909181027746 1105 4 0.10050196908819231 1105 5 0.027559129124320236 1105 6 -0.0020577378062043294 1105 7 0.06291187747418547 1105 8 0.11524667847298531 1105 9 0.04983259757120351 1105 10-11 0.0761669034770236 1105 12-13 0.08025000401063664 1105 14-15 0.12611099926095903 1105 16-17 0.21182144351814003 1105 18-19 0.1660052197791302 1105 20-21 0.1729996975250927 1105 22-23 0.06877338208835226 1105 24-25 0.12795787908827805 1105 26-27 0.1983388564513504 1105 28-29 0.051359851466713735 1105 30-31 0.10677089863931855 1105 32-33 0.17610670556670271 1105 34-35 0.1285817351110552 1105 36-37 0.22285300941570796 1105 38-39 0.1406992049783966 1105 40-41 0.25557283656129215 1105 42-43 0.3056797729539653 1105 44-45 0.34948142534977933 1105 46-47 0.28005425297978803 1105 48-49 0.33781757528802103 1105 50-51 0.39404404841113205 1105 52-53 0.31502101619241785 1105 54-55 0.3806942497117731 1105 56-57 0.3702935588264893 1105 58-59 0.42814780598352087 1105 60-61 0.4014700861777172 1105 62-63 0.3419168440607052 1105 64-65 0.290036052354651 1105 66-67 0.30108913362905554 1105 68-69 0.16656366185091542 1105 70-71 0.2182574511120876 1105 72-73 0.21136703818464042 1105 74-75 0.2596527423588171 1105 76-77 0.14649624148737672 1105 78-79 0.04004707236956051 1105 80-81 0.1609960213480477 1105 82-83 0.24185975673852056 1105 84-85 0.2219825855753612 1105 86-87 0.1764384404693793 1105 88-89 0.2512017442481138 1105 90-91 0.3992304179084556 1105 92-93 0.3926782142752643 1105 94-95 0.3178567998914268 1105 96-97 0.2321508737602791 1105 98-99 0.17832771301180728 1105 100-101 0.4139820089709261 1106 1 -0.10692345448743623 1106 2 -0.022124032479219125 1106 3 -0.1183158761552292 1106 4 -0.0643300410868406 1106 5 -0.05938859121146578 1106 6 -0.08378341342795181 1106 7 0.018367443428488173 1106 8 -0.010024143086660331 1106 9 -0.053478023010853803 1106 10-11 -0.013700154193962533 1106 12-13 0.020779971067923952 1106 14-15 0.08370835663470899 1106 16-17 -0.055080847023994295 1106 18-19 0.07315976861583096 1106 20-21 0.05269892628708561 1106 22-23 0.019315701054679835 1106 24-25 0.052920514402508445 1106 26-27 -0.07499444040769987 1106 28-29 -0.02354395297276568 1106 30-31 -0.047924532506037565 1106 32-33 -0.052131341917665 1106 34-35 0.01164459017211783 1106 36-37 -0.08369672069604661 1106 38-39 0.06341368342403086 1106 40-41 0.0055227916979205816 1106 42-43 -0.0820089331916094 1106 44-45 -0.14297773096141242 1106 46-47 -0.04169511590781383 1106 48-49 -0.06532566051307498 1106 50-51 -0.07529792117446021 1106 52-53 -0.2846041461717874 1106 54-55 -0.28180737553941526 1106 56-57 -0.10908876204507578 1106 58-59 -0.09554513804476272 1106 60-61 -0.14350640721893626 1106 62-63 -0.18841211321674933 1106 64-65 -0.16469474811857054 1106 66-67 -0.19695477196862754 1106 68-69 -0.17875925142311644 1106 70-71 -0.2406475093476459 1106 72-73 -0.3293554809096477 1106 74-75 -0.10572979349647937 1106 76-77 -0.12514470222679108 1106 78-79 -0.3254022436088775 1106 80-81 -0.25810782480085237 1106 82-83 -0.1922063771461282 1106 84-85 -0.0690912130582717 1106 86-87 -0.06586205270584955 1106 88-89 -0.002054872937968355 1106 90-91 0.11996929893749453 1106 92-93 0.04468280874773711 1106 94-95 -0.044064594593404394 1106 96-97 -0.02834261686733086 1106 98-99 -0.05528058278519765 1106 100-101 0.1695411726198799 1107 1 0.011055870143003688 1107 2 0.10265981243646394 1107 3 0.07696351400257129 1107 4 0.052014608107867844 1107 5 0.05543482741882144 1107 6 0.09588469265754185 1107 7 0.07103827653487116 1107 8 0.0750473138161496 1107 9 0.13044167606451396 1107 10-11 0.07471934656171442 1107 12-13 0.12474175334532589 1107 14-15 0.10240512400722679 1107 16-17 0.14980593699935696 1107 18-19 0.13720775228778592 1107 20-21 0.15009669474793697 1107 22-23 0.13257762370967896 1107 24-25 0.1565730382752193 1107 26-27 0.2882466812362807 1107 28-29 0.2095870954015311 1107 30-31 0.14962408923067017 1107 32-33 0.06236677897413756 1107 34-35 0.045962833276959714 1107 36-37 0.02644738937656399 1107 38-39 0.13513799200343612 1107 40-41 0.09658644345991974 1107 42-43 -0.05799111683329272 1107 44-45 -0.13323959981779865 1107 46-47 -0.10830496546220303 1107 48-49 -0.16321789156101119 1107 50-51 -0.10920306789594036 1107 52-53 0.05226889548403335 1107 54-55 0.05433579289063317 1107 56-57 0.05024481031887973 1107 58-59 -0.13665695793105925 1107 60-61 0.053614164797174624 1107 62-63 -0.04764548986742767 1107 64-65 -0.063215052745619 1107 66-67 -0.1596909720432791 1107 68-69 0.016316305534402886 1107 70-71 0.14341575847281263 1107 72-73 -0.04566218705534908 1107 74-75 -0.0652818516604512 1107 76-77 -0.11295464146778755 1107 78-79 -0.05047185598830595 1107 80-81 0.07340340122912181 1107 82-83 0.23878062999104088 1107 84-85 0.05485232113541372 1107 86-87 0.089347573033006 1107 88-89 0.14920523393077545 1107 90-91 0.012190739984756505 1107 92-93 0.3108268994394763 1107 94-95 0.30678355201144925 1107 96-97 0.03387537906469262 1107 98-99 0.021188778912804906 1107 100-101 0.19282243269667632 1108 1 -0.05915960402266762 1108 2 0.04860165603227529 1108 3 0.06055247936000541 1108 4 0.059652230127007044 1108 5 0.1122278397108758 1108 6 0.0648406991316719 1108 7 0.13197923536625922 1108 8 -0.023971278265669582 1108 9 0.025409218347235196 1108 10-11 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-0.8035296681008788 2316 96-97 -0.7945595223708573 2316 98-99 -1.143069758372608 2316 100-101 -1.2317735903576867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 15.0 5 42.0 6 67.0 7 151.0 8 371.0 9 669.0 10 1334.0 11 2381.0 12 3948.0 13 6232.0 14 7778.0 15 9798.0 16 12520.0 17 17470.0 18 16739.0 19 10831.0 20 8495.0 21 8165.0 22 8823.0 23 9881.0 24 11432.0 25 13905.0 26 17229.0 27 21461.0 28 27309.0 29 34432.0 30 43264.0 31 54397.0 32 69340.0 33 88908.0 34 119966.0 35 172821.0 36 288282.0 37 572889.0 38 870164.0 39 201463.0 40 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.091343215243015 18.447902778562504 13.352171625598846 23.108582380595628 2 14.404257063267496 21.285169956100226 41.101549154135206 23.20902382649708 3 20.94213119222586 25.61608977138226 29.295889563930462 24.145889472461413 4 11.82230125147951 15.785033102648393 39.64861250601409 32.744053139858 5 12.488817908907805 39.47010151234924 35.175337017706575 12.86574356103638 6 30.614946408284897 36.51045582685275 18.60081626978247 14.273781495079879 7 27.331646644092107 33.43753487257447 21.189756198400023 18.0410622849334 8 23.79033098989633 35.07735537371509 21.08090803153121 20.051405604857372 9 25.66778807719256 17.51196872490318 21.24862567755697 35.57161752034729 10-11 23.64921625345663 25.950879460140193 28.819104229033567 21.58080005736962 12-13 27.119448375582294 23.34111674235428 27.73009596204253 21.8093389200209 14-15 23.249620860796917 25.159915336249366 28.000834197715836 23.58962960523788 16-17 20.886881923905708 28.181015411347023 29.283114327670084 21.648988337077185 18-19 20.037868185784614 29.104024089288426 30.221098982315375 20.637008742611588 20-21 22.180506215599614 27.616057363564234 28.636010981414028 21.56742543942212 22-23 20.824348781183417 27.968707323948973 27.60755073745131 23.599393157416305 24-25 21.230590171480088 27.428636556392092 29.406491633819194 21.934281638308622 26-27 22.440883444839947 26.60593945365632 29.57206231100878 21.381114790494955 28-29 20.82264952574139 27.22316498286932 30.44345485066818 21.510730640721114 30-31 21.158882286576926 28.701067840264667 29.579144231337402 20.56090564182101 32-33 20.595747716894977 28.371586318932206 29.000354905748743 22.032311058424074 34-35 21.55142196880333 27.314156902461367 28.941935054253175 22.19248607448213 36-37 22.122738380403952 27.214513001968783 29.40016749824288 21.26258111938439 38-39 22.69321879455115 26.36767746690695 30.109841540822003 20.829262197719896 40-41 22.053590931193288 26.784617304069407 30.521460776562126 20.64033098817518 42-43 20.547672040742626 28.223137517338632 29.75594379777385 21.473246644144893 44-45 21.414463694407345 28.864473756425973 28.044309471103713 21.67675307806297 46-47 21.091262842196322 27.94649639854976 27.13917069307974 23.82307006617418 48-49 21.822700756730924 27.313048087424296 28.302494033921615 22.56175712192317 50-51 23.050607761491122 28.130813251469096 27.83381144391836 20.98476754312143 52-53 21.52679085002715 28.205311626858613 29.163855294722037 21.1040422283922 54-55 20.65740058617164 27.647536723537986 29.69891793688511 21.996144753405265 56-57 21.80894445035965 29.02136755420372 27.494567462438745 21.675120532997884 58-59 21.40711116526159 28.380211000267273 27.901528086261713 22.31114974820942 60-61 21.491344815039017 27.26677339409882 28.66357826675309 22.578303524109074 62-63 23.522509617970375 26.783600697360022 28.109718952203767 21.58417073246584 64-65 22.606875797503783 27.386035279966702 28.65847400652181 21.3486149160077 66-67 21.006858533395995 27.609717600239573 29.423183273454633 21.960240592909795 68-69 21.248936672319456 28.925019162765516 28.56909480400927 21.256949360905764 70-71 21.33255621102383 28.313357375097436 27.455266531182488 22.89881988269624 72-73 21.179074371589756 27.54818930843682 27.45309676425574 23.819639555717682 74-75 21.90354405972563 27.92526247043263 27.4856988143782 22.685494655463536 76-77 22.533279074968195 26.731886688003403 28.322280024489586 22.412554212538815 78-79 21.53740499954217 26.697317095504076 29.045453713029946 22.719824191923816 80-81 21.18487700575456 28.759966573196504 28.048933532762366 22.00622288828657 82-83 21.410800299286727 28.386668935099053 27.747739342483897 22.454791423130327 84-85 21.87991649189872 27.128132102615737 27.597426118161184 23.39452528732436 86-87 22.129276104004813 27.43157638305686 28.201822309693743 22.237325203244588 88-89 23.406117201738244 26.139142350626887 28.39785496559564 22.056885482039227 90-91 21.87280926160692 27.053871151732885 29.060064495338295 22.013255091321902 92-93 21.143165708001177 28.2396450274132 28.707617147614055 21.909572116971564 94-95 21.118493868672694 28.93613284572984 27.418018766270475 22.527354519326988 96-97 20.908908963114495 27.95019829284115 27.025252471662913 24.11564027238144 98-99 21.877592656927835 27.33903276505495 27.343075823443847 23.440298754573366 100-101 22.558551999114574 27.83303864866422 27.367604565798224 22.24080478642298 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 313.0 1 191.5 2 133.5 3 208.5 4 141.0 5 93.5 6 217.5 7 376.0 8 449.0 9 460.0 10 431.5 11 416.0 12 498.5 13 634.0 14 776.5 15 931.5 16 1110.0 17 1380.0 18 1709.5 19 2022.0 20 2488.0 21 3154.0 22 3975.5 23 5293.5 24 6973.0 25 9294.5 26 12575.5 27 15963.5 28 20046.0 29 24725.0 30 30293.5 31 37522.0 32 44487.5 33 51987.5 34 60633.5 35 69213.0 36 76299.0 37 82038.0 38 86420.0 39 88933.0 40 90617.5 41 92626.5 42 94801.5 43 97194.5 44 101551.5 45 104671.0 46 109142.5 47 125236.5 48 221580.0 49 217184.5 50 119279.0 51 101011.5 52 86591.0 53 76290.0 54 67497.0 55 59406.5 56 51377.0 57 43433.0 58 34864.0 59 26851.0 60 20216.0 61 14763.0 62 10491.0 63 6878.0 64 4143.5 65 2505.0 66 1722.5 67 1573.5 68 1269.5 69 842.5 70 701.5 71 605.5 72 446.0 73 293.0 74 171.5 75 131.0 76 122.0 77 99.5 78 67.5 79 63.0 80 59.5 81 48.5 82 29.5 83 24.5 84 18.0 85 9.0 86 6.0 87 3.0 88 2.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27480960717702735 2 0.021111056229682436 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.0246381027124234E-4 12-13 3.658761911556749E-5 14-15 0.0 16-17 6.036957154068634E-4 18-19 0.0 20-21 5.488142867335122E-5 22-23 5.488142867335122E-5 24-25 0.002853834291014264 26-27 0.0025062519094163726 28-29 0.0020854942895873465 30-31 0.0011525100021403758 32-33 0.0016464428602005368 34-35 0.0019391438131250768 36-37 7.317523823113498E-5 38-39 1.8293809557783744E-5 40-41 5.854019058490798E-4 42-43 0.0038782876262501535 44-45 0.00420757619829026 46-47 0.01379353240656894 48-49 0.015494856695442828 50-51 0.006768709536379984 52-53 0.007939513348078143 54-55 2.3781952425118864E-4 56-57 0.0035124114350944782 58-59 0.0014817985741804832 60-61 0.0012622728594870781 62-63 0.0 64-65 0.0010061595256781058 66-67 1.8293809557783744E-5 68-69 0.0 70-71 1.6464428602005367E-4 72-73 0.0013903295263915643 74-75 0.0 76-77 0.20031721465773197 78-79 0.10665290972187921 80-81 0.0010610409543514569 82-83 0.0 84-85 6.219895249646471E-4 86-87 0.12394055975398484 88-89 0.5958476711065742 90-91 0.3156413901100007 92-93 0.2967072972176945 94-95 0.002561133338089724 96-97 0.0031465352439388034 98-99 0.0031282414343810202 100-101 6.40283334522431E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2733165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.112337470753886 #Duplication Level Percentage of deduplicated Percentage of total 1 59.710213540799316 20.965651682953244 2 15.758495079994814 11.066351945599854 3 7.693160120992372 8.103745031544898 4 4.46865938391113 6.276203053189551 5 2.973230757853319 5.219854087408553 6 2.069892185033361 4.36072517573805 7 1.4796361730423018 3.6367439249257267 8 1.0848891039038133 3.047439386769158 9 0.8162702020932305 2.5795039322896187 >10 3.8570231403162807 21.864336033955315 >50 0.05962563881991184 1.3884059890422205 >100 0.021401240733667445 1.5129620463252915 >500 0.002709572849557414 0.6454844831720152 >1k 0.0031264302110198964 2.2706490155439947 >5k 9.379290633059689E-4 2.00506361991738 >10k+ 7.295003825713091E-4 5.056880591625189 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 36139 1.3222399672174934 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 30835 1.1281792354285234 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 19376 0.7089217079832355 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 14709 0.5381672895708821 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 12797 0.4682117618219171 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 12301 0.4500643027405956 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 12039 0.44047834653231693 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 8001 0.2927375405436554 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6739 0.24656396521980928 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6636 0.24279544045090581 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5989 0.21912325088313364 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5778 0.21140326324974892 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5645 0.20653710990737842 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5515 0.2017807194223547 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5456 0.1996220498945362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5036 0.18425524986599784 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4669 0.17082759365058459 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4655 0.17031536698296662 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3605 0.13189836691162077 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3375 0.12348321451504025 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3095 0.11323868116268138 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2806 0.10266485923828236 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2775 0.10153064304569977 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.317523823113496E-5 0.0 6 7.317523823113496E-5 0.0 0.0 7.317523823113496E-5 0.0 7 1.4635047646226993E-4 0.0 0.0 6.951647631957821E-4 0.0 8 2.9270095292453985E-4 0.0 0.0 6.951647631957821E-4 0.0 9 4.390514293868098E-4 0.0 0.0 0.0011708038116981594 0.0 10-11 4.5734523894459356E-4 0.0 0.0 0.0012622728594870783 0.0 12-13 4.756390485023773E-4 0.0 0.0 0.0013903295263915643 0.0 14-15 4.756390485023773E-4 0.0 0.0 0.0014452109550649155 0.0 16-17 7.317523823113497E-4 0.0 0.0 0.0018293809557783742 0.0 18-19 7.683400014269171E-4 0.0 0.0 0.0022501385756074003 0.0 20-21 8.781028587736196E-4 0.0 0.0 0.002780659052783129 0.0 22-23 0.001207391430813727 0.0 0.0 0.004353926674752531 0.0 24-25 0.001426917145507132 0.0 0.0 0.005799137629817446 0.0 26-27 0.0015000923837382668 0.0 0.0 0.006604065250359931 0.0 28-29 0.0017196180984316717 0.0 0.0 0.008524915253927224 0.0 30-31 0.0023050200042807516 0.0 0.0 0.01456187240799586 0.0 32-33 0.0024330766711852378 0.0 0.0 0.025794271476475075 0.0 34-35 0.0024513704807430214 0.0 0.0 0.04674068342013746 0.0 36-37 0.0027440714336675613 0.0 0.0 0.07816944824040993 0.0 38-39 0.002963597148360966 0.0 0.0 0.11942198879321228 0.0 40-41 0.003311179529958857 0.0 0.0 0.16308931220764206 0.0 42-43 0.0036587619115567484 0.0 0.0 0.20699445514632303 0.0 44-45 0.003841700007134586 0.0 0.0 0.2592598690529112 0.0 46-47 0.003914875245365721 0.0 0.0 0.3212210020251247 0.0 48-49 0.0041526947696169094 0.0 0.0 0.3987501669310122 0.0 50-51 0.004463689532099233 0.0 0.0 0.47000455515857986 0.0 52-53 0.004811271913697124 0.0 0.0 0.5372343052834352 0.0 54-55 0.004994210009274962 0.0 0.0 0.6088362758926007 0.0 56-57 0.0050490914379483125 0.0 0.0 0.6922011660474212 0.0 58-59 0.0050490914379483125 0.0 0.0 0.8041592805410578 0.0 60-61 0.00508567905706388 0.0 0.0 0.9455155469940526 0.0 62-63 0.00537838000998842 0.0 0.0 1.0714684257993938 0.0 64-65 0.005634493343797392 0.0 0.0 1.1836643598172816 0.0 66-67 0.005707668582028528 0.0 0.0 1.3149956186326108 0.0 68-69 0.005780843820259662 0.0 0.0 1.4640169912903174 0.0 70-71 0.005817431439375229 0.0 0.0 1.6318078125543098 0.0 72-73 0.0058540190584907975 0.0 0.0 1.811965249079364 0.0 74-75 0.005908900487164149 0.0 0.0 1.9974095965666177 0.0 76-77 0.005982075725395284 0.0 0.0 2.182579536910505 0.0 78-79 0.006000369534953067 0.0 0.0 2.400897860173096 0.0 80-81 0.006000369534953067 0.0 0.0 2.6397418377595203 0.0 82-83 0.006073544773184203 0.0 0.0 2.8818421134472305 0.0 84-85 0.00611013239229977 0.0 0.0 3.192928344977343 0.0 86-87 0.006165013820973121 0.0 0.0 3.5466208589675343 0.0 88-89 0.006183307630530904 0.0 0.0 3.8748118024341744 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCCCT 4040 0.0 29.707663 4 GTATAGC 2385 0.0 28.527649 1 ATAGCCC 2270 0.0 28.003237 3 GTGTAGC 2605 0.0 26.848988 1 GTAGCCC 2440 0.0 26.246613 3 GTACCGT 140 1.798641E-5 23.71916 6 TACGACT 160 1.882272E-6 23.71916 4 GTACATG 13180 0.0 23.609167 1 TACATGG 13025 0.0 23.496687 2 TATAGCC 2830 0.0 23.137548 2 ACATGGG 13375 0.0 22.096504 3 CCCTGGC 4960 0.0 21.615042 7 CCCTGCG 310 1.0913936E-11 21.423758 9 TATAACG 115 0.0037479433 20.629887 2 GTCCTAC 1275 0.0 20.524416 1 AGCCCTG 5695 0.0 20.24146 5 TGTAGCC 3130 0.0 20.161924 2 CATGGGG 4060 0.0 19.863337 4 TACGATA 120 0.004801097 19.765968 4 TACCGTC 120 0.004801097 19.765968 7 >>END_MODULE