FastQCFastQC Report
Thu 26 May 2016
SRR936374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57461
Sequences flagged as poor quality0
Sequence length101
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34546.011033570595709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24934.338594873044325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19003.306590557073493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14492.5217103774734166No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11271.9613302935904353No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8531.4844851290440473No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7431.2930509388976872No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7161.2460625467708533No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.5934459894537164No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2930.5099110701171229No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2860.49772889438053636No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.4751048537268756No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2620.4559614347122396No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2570.4472598806146778No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.40897304258540573No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.3689458937366214No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1830.3184768799707628No Hit
GTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.3167365691512504No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.31499625833173805No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1700.29585283931710205No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.2784497311219784No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.24016289309270636No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1350.23494196063416928No Hit
GGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.22450009571709506No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1180.20535667670245908No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1160.20187605506343434No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.18621325768782304No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.1844729468683107No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.18273263604879833No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1020.17751170359026122No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1000.1740310819512365No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1000.1740310819512365No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC980.17055046031221177No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG960.16706983867318703No Hit
GAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.16358921703416232No Hit
TATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.16358921703416232No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.16010859539513758No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA890.1548876629366005No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC880.15314735211708813No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.14792641965855102No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.14096517638050157No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.13226362228293972No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA750.13052331146342738No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA730.12704268982440264No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG720.12530237900489027No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.12530237900489027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG690.12008144654635318No Hit
GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.12008144654635318No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC690.12008144654635318No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA670.11660082490732844No Hit
GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.1148605140878161No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.10789927080976662No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT620.10789927080976662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC610.10615895999025425No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT600.10441864917074191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG590.10267833835122954No Hit
ACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.10093802753171717No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.10093802753171717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATAA200.002099453270.94492
ACATAAG200.002099453270.94493
CATAAGC200.002099453270.94494
GTACATA353.462952E-667.566581
CTGTCTA250.005082668356.7559249
AGTAAAA1950.031.5310692
GAGTAAA2000.030.7427941
CCACCAG350.00828638427.19243888-89
CAGAGAA906.872142E-926.2758910-11
GTATCAA25350.025.560691
AACGCAG26950.024.218676
TATCAAC26850.024.1327172
TCAACGC27600.023.4769384
ATCAACG27600.023.4769383
CAACGCA27750.023.3500355
GTAAAAA2653.092282E-1123.2021063
AGTACTC2354.645699E-922.1388362
TGGGGAA1503.068999E-522.0717496
ACGCAGA29500.021.8045357
CGCAGAG29700.021.4984578
Produced by FastQC (version 0.11.5)